MD12G1006200.v1.1

Glycosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0006924
Physical Location & Seq
Forward (+)
747702 .. 750220
2519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1006200.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGGCCGATTCTTCTTCGGTGGTGCTATACTATCTCTCTCAGCCGCGGTCCTTGAGACATTTTTACTTCATACCCACAACCTTAGCTCTCGCAACCTCTCTCCTCATCCTCTTCTACATTTCCTCCACCTCCAATCTCTTCGCCCACCACCATCATCATGCTCACTCAATCCCACAACTGCTACTCAATATTTCATCTTCCTATACACCCTTTCGAGTCGAAGCTCATCTGAAAGCGGACGGTGCTCAAGTAATCCAGGGTCAACGCTCACCGGAGCAACCTCAATTGGGATCGAATGGAAACTATGTCAATAACAAAGCCGTGTTCCATGATAGACATATCTTTCTTGAAGACTATAAAGAAATGAAAAGGAGCTTTAAGATATATGTTTATCCTCACAGGCAAGATGACTCCTTTGCAAATGCACTTTTGCCTGTGGATTCTGAACCCAGGGGTAATTACGCGAGTGAAAGTTACTTCAAGAAGGTCCTTATGAAAAGTCATTTCATTACCAATGATCCTACTAAGGCGGATCTTTTCTTTCTGCCTTTCTCAATTGCAAGGTTGCGGCATGACCCCAGAATTGGCGTGGGAGGTATCCAAGATTTTATAAGAGATTACATCTTCAATGTTAGTCACAAGTATGAATATTGGAACCGGACAGGTGGAGCAGATCATTTTTACGTTGCTTGTCATTCCATTGGGCGGTCAGCCATGGAAAAAGCAAATGAAGTCAAATTTAATGCCATTCAAGTTGTTTGCTCTTCAAGCTACTTCCTACCGGGCTACATACCACACAAGGATGCATGTCTGCCACAAATTTGGCCCAGAAATGAAAAGCCCCCTGACCTTCGTTCTTCAAATAGGACGAAACTTGCATTCTTCGCCGGAGGAATCAACTCCCCAGTACGAGAAAAGCTTCTTCAAGTCTGGAGAAATGACTCTGAGATTTTTGCGCACTTTGGTCGGCTTACTACACCATATGCTGATGAACTGCTTGGCAGCAGGTTCTGCCTCCATGTCAAAGGCTTTGAAGTAAATACAGCTCGTATTGCAGATTCACTATACTACGGTTGTGTCCCAGTGATAATTGCCAACTACTATGATTTACCATTCGCAGACGTAATAAACTGGAGCAGCTTCTCGGTCATTGTTGCTACTCTAGATATCCCACTGCTTAAGAAAATCCTCAAGGAGATCAGCTCCGATGAATATCTGCGGTTACAGAGTAATGTGTTGAAGGTGCGCAAACATTTCCAGTGGCATCTTTCCCCTATTGATTATGATGCTTTTTATATGGTTATGTATGAGTTGTGGCTCCGACGAGGTTTTTCGACAGTTCCCTTGAGCAATTTTGAAACTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00534 ko01100 map00534 map01100
Pfam Domains
Protein Families
Exostosin

Protein Analysis

456

Amino Acids

52.42

Weight (kDa)

8.83

Isoelectric Point (pI)

51.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exostosin_GT47 PF03016 123 - 399 3.7e-69 Exostosin GT47 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 611
Acc16I TGCGCA 2 cut(s) 957, 1247
Acc36I ACCTGC 1 cut(s) 996
AccII CGCG 2 cut(s) 46, 464
AciI CCGC 7 cut(s) 44, 46, 236, 530, 568, 706, 1219
AclWI GGATC 3 cut(s) 298, 512, 540
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 737, 819
AfaI GTAC 1 cut(s) 909
AfiI CCNNNNNNNGG 3 cut(s) 287, 584, 705
AflII CTTAAG 1 cut(s) 1178
AjnI CCWGG 2 cut(s) 255, 449
AluBI AGCT 8 cut(s) 86, 224, 375, 771, 919, 1046, 1140, 1203
AluI AGCT 8 cut(s) 86, 224, 375, 771, 919, 1046, 1140, 1203
Alw21I GWGCWC 1 cut(s) 247
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 3 cut(s) 298, 512, 540
AlwNI CAGNNNCTG 1 cut(s) 1011
AoxI GGCC 2 cut(s) 3, 824
ApeKI GCWGC 2 cut(s) 1002, 1137
ApoI RAATTY 2 cut(s) 737, 819
Asp700I GAANNNNTTC 1 cut(s) 918
AspLEI GCGC 2 cut(s) 958, 1248
AspS9I GGNCC 3 cut(s) 48, 487, 825
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 1 cut(s) 261
AvaII GGWCC 2 cut(s) 48, 487
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 2 cut(s) 1014, 1149
BccI CCATC 1 cut(s) 159
BceAI ACGGC 1 cut(s) 305
BcgI CGANNNNNNTGC 2 cut(s) 947, 981
BciT130I CCWGG 2 cut(s) 257, 451
BciVI GTATCC 1 cut(s) 608
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 1 cut(s) 1163
BfrI CTTAAG 1 cut(s) 1178
BfuAI ACCTGC 1 cut(s) 996
BfuI GTATCC 1 cut(s) 608
BisI GCNGC 4 cut(s) 44, 569, 1003, 1138
BlsI GCNGC 4 cut(s) 45, 570, 1004, 1139
Bme1390I CCNGG 3 cut(s) 257, 451, 783
Bme18I GGWCC 2 cut(s) 48, 487
BmgT120I GGNCC 3 cut(s) 48, 487, 825
BmiI GGNNCC 2 cut(s) 656, 1319
BmrFI CCNGG 3 cut(s) 257, 451, 783
BmrI ACTGGG 2 cut(s) 899, 1076
BmsI GCATC 3 cut(s) 793, 1273, 1276
BmuI ACTGGG 2 cut(s) 899, 1076
BpiI GAAGAC 1 cut(s) 357
BplI GAGNNNNNCTC 2 cut(s) 1187, 1219
BpmI CTGGAG 2 cut(s) 952, 1153
Bpu10I CCTNAGC 1 cut(s) 82
BpuEI CTTGAG 4 cut(s) 73, 231, 1175, 1366
BpuMI CCSGG 1 cut(s) 783
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 5 cut(s) 44, 256, 449, 450, 714
BsaWI WCCGGW 2 cut(s) 271, 657
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 584, 705
Bse1I ACTGG 4 cut(s) 905, 1082, 1136, 1258
Bse8I GATNNNNATC 1 cut(s) 1212
BseBI CCWGG 2 cut(s) 257, 451
BseDI CCNNGG 5 cut(s) 44, 256, 449, 450, 714
BseGI GGATG 2 cut(s) 105, 808
BseJI GATNNNNATC 1 cut(s) 1212
BseLI CCNNNNNNNGG 3 cut(s) 287, 584, 705
BseMII CTCAG 2 cut(s) 53, 936
BseNI ACTGG 4 cut(s) 905, 1082, 1136, 1258
BseRI GAGGAG 1 cut(s) 92
BseXI GCAGC 2 cut(s) 1014, 1149
Bsh1236I CGCG 2 cut(s) 46, 464
BshFI GGCC 2 cut(s) 5, 826
BsiHKAI GWGCWC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 272, 658, 782, 888
BslFI GGGAC 1 cut(s) 1064
BslI CCNNNNNNNGG 3 cut(s) 287, 584, 705
BsmAI GTCTC 1 cut(s) 49
BsmFI GGGAC 1 cut(s) 1064
BsmI GAATGC 1 cut(s) 878
BsnI GGCC 2 cut(s) 5, 826
Bsp1286I GDGCHC 1 cut(s) 247
Bsp143I GATC 5 cut(s) 290, 517, 532, 673, 1197
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 7 cut(s) 44, 46, 236, 530, 568, 706, 1219
BspANI GGCC 2 cut(s) 5, 826
BspCNI CTCAG 2 cut(s) 52, 937
BspFNI CGCG 2 cut(s) 46, 464
BspLI GGNNCC 2 cut(s) 656, 1319
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 3 cut(s) 298, 512, 540
BspQI GCTCTTC 1 cut(s) 769
BspTI CTTAAG 1 cut(s) 1178
BsrI ACTGG 4 cut(s) 905, 1082, 1136, 1258
BssECI CCNNGG 5 cut(s) 44, 256, 449, 450, 714
BssMI GATC 5 cut(s) 290, 517, 532, 673, 1197
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 257, 451
Bst4CI ACNGT 3 cut(s) 242, 1073, 1339
Bst6I CTCTTC 3 cut(s) 116, 143, 769
BstAFI CTTAAG 1 cut(s) 1178
BstAPI GCANNNNNTGC 1 cut(s) 1011
BstDEI CTNAG 5 cut(s) 39, 82, 525, 945, 1365
BstDSI CCRYGG 2 cut(s) 44, 714
BstF5I GGATG 2 cut(s) 105, 808
BstFNI CGCG 2 cut(s) 46, 464
BstHHI GCGC 2 cut(s) 958, 1248
BstKTI GATC 5 cut(s) 293, 520, 535, 676, 1200
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 5 cut(s) 290, 517, 532, 673, 1197
BstMWI GCNNNNNNNGC 5 cut(s) 242, 768, 884, 1011, 1052
BstNI CCWGG 2 cut(s) 257, 451
BstNSI RCATGY 1 cut(s) 810
BstSCI CCNGG 3 cut(s) 255, 449, 781
BstUI CGCG 2 cut(s) 46, 464
BstV1I GCAGC 2 cut(s) 1014, 1149
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 532
BstXI CCANNNNNNTGG 1 cut(s) 822
BstYI RGATCY 1 cut(s) 532
BsuI GTATCC 1 cut(s) 608
BsuRI GGCC 2 cut(s) 5, 826
BtgI CCRYGG 2 cut(s) 44, 714
BtsCI GGATG 2 cut(s) 105, 808
BtsI GCAGTG 1 cut(s) 1172
BtsIMutI CAGTG 3 cut(s) 1089, 1172, 1265
BveI ACCTGC 1 cut(s) 996
CaiI CAGNNNCTG 1 cut(s) 1011
CfoI GCGC 2 cut(s) 958, 1248
Cfr13I GGNCC 3 cut(s) 48, 487, 825
Cfr42I CCGCGG 1 cut(s) 47
Csp6I GTAC 1 cut(s) 908
CviAII CATG 6 cut(s) 158, 329, 572, 715, 807, 1019
CviQI GTAC 1 cut(s) 908
DdeI CTNAG 5 cut(s) 39, 82, 525, 945, 1365
DpnI GATC 5 cut(s) 292, 519, 534, 675, 1199
DpnII GATC 5 cut(s) 290, 517, 532, 673, 1197
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 116, 143, 769
EarI CTCTTC 3 cut(s) 116, 143, 769
EciI GGCGGA 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 714
Eco32I GATATC 1 cut(s) 1168
Eco47I GGWCC 2 cut(s) 48, 487
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 2 cut(s) 255, 449
EcoRV GATATC 1 cut(s) 1168
EcoT14I CCWWGG 1 cut(s) 714
EcoT22I ATGCAT 1 cut(s) 808
ErhI CCWWGG 1 cut(s) 714
FaeI CATG 6 cut(s) 161, 332, 575, 718, 810, 1022
FaqI GGGAC 1 cut(s) 1064
FatI CATG 6 cut(s) 157, 328, 571, 714, 806, 1018
FauNDI CATATG 1 cut(s) 982
Fnu4HI GCNGC 4 cut(s) 44, 569, 1003, 1138
FokI GGATG 2 cut(s) 92, 815
Fsp4HI GCNGC 4 cut(s) 44, 569, 1003, 1138
FspBI CTAG 1 cut(s) 1163
FspI TGCGCA 2 cut(s) 957, 1247
GlaI GCGC 2 cut(s) 957, 1247
GluI GCNGC 4 cut(s) 44, 569, 1003, 1138
GsuI CTGGAG 2 cut(s) 952, 1153
HaeIII GGCC 2 cut(s) 5, 826
HapII CCGG 4 cut(s) 272, 658, 782, 888
HhaI GCGC 2 cut(s) 958, 1248
Hin1II CATG 6 cut(s) 161, 332, 575, 718, 810, 1022
Hin6I GCGC 2 cut(s) 956, 1246
HinP1I GCGC 2 cut(s) 956, 1246
HincII GTYRAC 1 cut(s) 263
HindII GTYRAC 1 cut(s) 263
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 7 cut(s) 8, 216, 410, 440, 894, 941, 1058
HpaII CCGG 4 cut(s) 272, 658, 782, 888
HphI GGTGA 1 cut(s) 261
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 5 cut(s) 231, 445, 946, 1207, 1322
Hpy188III TCNNGA 4 cut(s) 347, 481, 931, 1163
Hpy8I GTNNAC 1 cut(s) 263
Hpy99I CGWCG 1 cut(s) 1326
HpyAV CCTTC 3 cut(s) 478, 860, 1234
HpyCH4III ACNGT 3 cut(s) 242, 1073, 1339
HpyCH4IV ACGT 2 cut(s) 684, 1122
HpyCH4V TGCA 6 cut(s) 419, 425, 560, 806, 878, 1055
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 768, 884, 1011, 1052
HpyF3I CTNAG 5 cut(s) 39, 82, 525, 945, 1365
HpySE526I ACGT 2 cut(s) 684, 1122
Hsp92II CATG 6 cut(s) 161, 332, 575, 718, 810, 1022
HspAI GCGC 2 cut(s) 956, 1246
KspI CCGCGG 1 cut(s) 47
Kzo9I GATC 5 cut(s) 290, 517, 532, 673, 1197
LguI GCTCTTC 1 cut(s) 769
LmnI GCTCC 6 cut(s) 274, 372, 668, 1134, 1208, 1323
Lsp1109I GCAGC 2 cut(s) 1014, 1149
LweI GCATC 3 cut(s) 793, 1273, 1276
MaeI CTAG 1 cut(s) 1163
MaeII ACGT 2 cut(s) 684, 1122
MaeIII GTNAC 3 cut(s) 473, 635, 1221
MalI GATC 5 cut(s) 292, 519, 534, 675, 1199
MboI GATC 5 cut(s) 290, 517, 532, 673, 1197
MfeI CAATTG 2 cut(s) 284, 555
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 1 cut(s) 247
MluCI AATT 8 cut(s) 284, 457, 555, 582, 737, 819, 1089, 1351
MlyI GAGTC 3 cut(s) 225, 404, 935
MmeI TCCRAC 1 cut(s) 1345
Mph1103I ATGCAT 1 cut(s) 808
MroXI GAANNNNTTC 1 cut(s) 918
MseI TTAA 3 cut(s) 378, 741, 1179
MslI CAYNNNNRTG 3 cut(s) 156, 642, 801
MspA1I CMGCKG 1 cut(s) 46
MspCI CTTAAG 1 cut(s) 1178
MspI CCGG 4 cut(s) 272, 658, 782, 888
MspR9I CCNGG 3 cut(s) 257, 451, 783
MunI CAATTG 2 cut(s) 284, 555
Mva1269I GAATGC 1 cut(s) 878
MvaI CCWGG 2 cut(s) 257, 451
MvnI CGCG 2 cut(s) 46, 464
MwoI GCNNNNNNNGC 5 cut(s) 242, 768, 884, 1011, 1052
NciI CCSGG 1 cut(s) 783
NcoI CCATGG 1 cut(s) 714
NdeI CATATG 1 cut(s) 982
NdeII GATC 5 cut(s) 290, 517, 532, 673, 1197
NlaIII CATG 6 cut(s) 161, 332, 575, 718, 810, 1022
NlaIV GGNNCC 2 cut(s) 656, 1319
NmuCI GTSAC 1 cut(s) 635
NsbI TGCGCA 2 cut(s) 957, 1247
NsiI ATGCAT 1 cut(s) 808
NspI RCATGY 1 cut(s) 810
PasI CCCWGGG 1 cut(s) 450
PciSI GCTCTTC 1 cut(s) 769
PctI GAATGC 1 cut(s) 878
PdmI GAANNNNTTC 1 cut(s) 918
PfeI GAWTC 4 cut(s) 8, 440, 894, 1058
PkrI GCNGC 4 cut(s) 45, 570, 1004, 1139
PleI GAGTC 3 cut(s) 224, 404, 935
PpsI GAGTC 3 cut(s) 224, 404, 935
PpuMI RGGWCCY 1 cut(s) 487
PsiI TTATAA 1 cut(s) 611
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 255, 449
PspGI CCWGG 2 cut(s) 255, 449
PspN4I GGNNCC 2 cut(s) 656, 1319
PspPI GGNCC 3 cut(s) 48, 487, 825
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 1011
PsuI RGATCY 1 cut(s) 532
RsaI GTAC 1 cut(s) 909
RsaNI GTAC 1 cut(s) 908
RseI CAYNNNNRTG 3 cut(s) 156, 642, 801
SacII CCGCGG 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 769
SaqAI TTAA 3 cut(s) 378, 741, 1179
SatI GCNGC 4 cut(s) 44, 569, 1003, 1138
Sau3AI GATC 5 cut(s) 290, 517, 532, 673, 1197
Sau96I GGNCC 3 cut(s) 48, 487, 825
SchI GAGTC 3 cut(s) 225, 404, 935
ScrFI CCNGG 3 cut(s) 257, 451, 783
SduI GDGCHC 1 cut(s) 247
SfaNI GCATC 3 cut(s) 793, 1273, 1276
Sfr303I CCGCGG 1 cut(s) 47
SgrBI CCGCGG 1 cut(s) 47
SinI GGWCC 2 cut(s) 48, 487
SmiMI CAYNNNNRTG 3 cut(s) 156, 642, 801
SmlI CTYRAG 5 cut(s) 52, 246, 1178, 1190, 1345
SmoI CTYRAG 5 cut(s) 52, 246, 1178, 1190, 1345
Sse9I AATT 8 cut(s) 284, 457, 555, 582, 737, 819, 1089, 1351
SsiI CCGC 7 cut(s) 44, 46, 236, 530, 568, 706, 1219
SspI AATATT 2 cut(s) 190, 650
SspMI CTAG 1 cut(s) 1163
StyD4I CCNGG 3 cut(s) 255, 449, 781
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 3 cut(s) 242, 1073, 1339
TaiI ACGT 2 cut(s) 687, 1125
TaqI TCGA 4 cut(s) 214, 219, 293, 1334
TaqII GACCGA 1 cut(s) 1135
TasI AATT 8 cut(s) 284, 457, 555, 582, 737, 819, 1089, 1351
TauI GCSGC 2 cut(s) 46, 571
TfiI GAWTC 4 cut(s) 8, 440, 894, 1058
Tru1I TTAA 3 cut(s) 378, 741, 1179
Tru9I TTAA 3 cut(s) 378, 741, 1179
TscAI CASTG 3 cut(s) 1089, 1179, 1265
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 2 cut(s) 1002, 1137
Tsp45I GTSAC 1 cut(s) 635
TspRI CASTG 3 cut(s) 1089, 1179, 1265
Vha464I CTTAAG 1 cut(s) 1178
VpaK11BI GGWCC 2 cut(s) 48, 487
XapI RAATTY 2 cut(s) 737, 819
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 1 cut(s) 810
XcmI CCANNNNNNNNNTGG 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 918
XspI CTAG 1 cut(s) 1163
Zsp2I ATGCAT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.