Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0006924

Physical Location & Seq

Genomic Coordinates Forward (+)

442094 .. 444310

Gene Structure 2217 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g00450.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGCCGATTCTTCTTCGGCGGTGCTATACTATCTGTCTCAGCCGCGGTCCTTGAGACATTTTTACTTCGTACCCACAATCTTAGCTCTCGTAACGTCTCTCCTCATCCTCTTCTACATTTCCTCTACCTCCAATCTCTTCGCCCACCACCATCATCATGCTCACTCAATCCCACAACTGCTACTCAATATTTCATCTTCCTATACACCCCTTCGAGTCGAAGAAGCTCATCTGAAAGCGGACGGTGCTCAAGTAATCGAGGGTCAACGCTCACCAGAGCAACCTCAATTGGGATCGAATGGAAATTATGGCAATAACAACGCCGTGTTCCATGATAGACATATCTTTCTTGAAGACTATAAAGAAATGAAAAGGAGCTTTAAGATATATGTTTATCCTCACAGGCAAGATGACTCCTTTGCAAATGCACTTTTGCCTGTGGATTCTGAACCCGGGGGTAATTACGCGAGTGAAAGTTACTTCAAGAAGGTCCTTATGAAAAGTCATTTCATTACCAATGATCCTACTAAGGCGGATCTTTTCTTTCTGCCTTTCTCAATTGCAAGGTTGCGGCATGACCCCAGAATTGGCGTGGGAGGTATCCAAGATTTTATAAGAGATTACATCTTCAATGTTAGTCACAAGTACGAATATTGGAACCGGACAGGTGGAGCAGATCATTTTTACGTTGCTTGTCATTCCATTGGGCGGTCAGCCATGGAAAAAGCAAATGAAGTCAAATTTAATGCCATTCAAGTTGTTTGCTCTTCAAGCTACTTCCTACCGGGCTACATACCACACAAGGATGCATGTCTGCCACAAATTTGGCCCAGAAATGAAAAGCCCCCTGACCTTCGTTCTTCAAACAGGACGAAACTTGCATTCTTCGCCGGAGGAATCAACTCCCCAGTACGAGAAAAGCTTCTTCAAGTCTGGAGAAATGACTCCGAGATTTTTGCGCACTTTGGTCGGCTTACTACACCATATGCTGATGAACTGCTTGGCAGCAGGTTCTGCCTCCATGTCAAAGGCTTTGAAGTAAATACAGCTCGTATTGCAGATTCACTATACTACGGTTGTGTCCCAGTGATAATTGCCAACTACTATGATTTACCATTCGCAGACATAATAAACTGGAGCAGCTTCTCAGTCATTGTTGCTACTCTAGATATCCCACTGCTTAAGAAAATCCTTAAGGAGATCAGCTCCGATGAATATCTGCGGTTACAGAGTAATGTGTTGAGGGTGCGCAAACATTTCCAGTGGCATCTTTCCCCTATTGATTATGATGCTTTTTACATGGTTATGTATGAGTTGTGGCTCCGACGAGGTTTTTCGACAGTTCCCTTGAGCAATTTTGAAACTTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00534 ko01100 map00534 map01100

Pfam Domains

Protein Families

Exostosin

Protein Analysis

457

Amino Acids

52.4

Weight (kDa)

8.13

Isoelectric Point (pI)

54.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006924)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g27020
malus_domestica	MD12G1006200.v1.1
prunus_persica	Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1 Prupe.7G006300_v2.0.a1
pyrus_communis	pycom12g00450
rosa_chinensis	RchiOBHm_Chr3g0496811
rosa_laevigata	RLG00000022501
rosa_multiflora	Rmu_sc0004684.1_g000007 Rmu_sc0011141.1_g000003
rosa_roxburghii	Rroxscaffold_6G00388690
rosa_rugosa	Rorug03G0288200
rosa_samantha	Rh3AG331700 Rh3BG368200 Rh3CG364500 Rh3DG367700
rosa_wichuraiana	Rw3G029020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	614
Acc16I	TGCGCA	2 cut(s)	960, 1250
Acc36I	ACCTGC	1 cut(s)	999
AccII	CGCG	2 cut(s)	46, 467
AciI	CCGC	8 cut(s)	20, 44, 46, 239, 533, 571, 709, 1222
AclWI	GGATC	3 cut(s)	301, 515, 543
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	2 cut(s)	740, 822
AfaI	GTAC	3 cut(s)	72, 647, 912
AfiI	CCNNNNNNNGG	3 cut(s)	290, 587, 708
AflII	CTTAAG	2 cut(s)	1181, 1193
AgsI	TTSAA	9 cut(s)	353, 484, 631, 755, 771, 864, 929, 1037, 1361
AluBI	AGCT	8 cut(s)	86, 227, 378, 774, 922, 1049, 1143, 1206
AluI	AGCT	8 cut(s)	86, 227, 378, 774, 922, 1049, 1143, 1206
Alw21I	GWGCWC	1 cut(s)	250
Alw26I	GTCTC	3 cut(s)	42, 49, 102
AlwI	GGATC	3 cut(s)	301, 515, 543
AlwNI	CAGNNNCTG	1 cut(s)	1014
Ama87I	CYCGRG	1 cut(s)	452
AoxI	GGCC	2 cut(s)	3, 827
ApeKI	GCWGC	2 cut(s)	1005, 1140
ApoI	RAATTY	2 cut(s)	740, 822
Asp700I	GAANNNNTTC	1 cut(s)	921
AspLEI	GCGC	2 cut(s)	961, 1251
AspS9I	GGNCC	3 cut(s)	48, 490, 828
AsuC2I	CCSGG	3 cut(s)	453, 454, 786
AsuHPI	GGTGA	1 cut(s)	264
AvaI	CYCGRG	1 cut(s)	452
AvaII	GGWCC	2 cut(s)	48, 490
BbsI	GAAGAC	1 cut(s)	360
Bbv12I	GWGCWC	1 cut(s)	250
BbvI	GCAGC	2 cut(s)	1017, 1152
BccI	CCATC	1 cut(s)	159
BceAI	ACGGC	1 cut(s)	308
BcgI	CGANNNNNNTGC	4 cut(s)	938, 950, 972, 984
BciVI	GTATCC	1 cut(s)	611
BcnI	CCSGG	3 cut(s)	453, 454, 786
BcoDI	GTCTC	3 cut(s)	42, 49, 102
BfaI	CTAG	1 cut(s)	1166
BfrI	CTTAAG	2 cut(s)	1181, 1193
BfuAI	ACCTGC	1 cut(s)	999
BfuI	GTATCC	1 cut(s)	611
BisI	GCNGC	4 cut(s)	44, 572, 1006, 1141
BlsI	GCNGC	4 cut(s)	45, 573, 1007, 1142
Bme1390I	CCNGG	3 cut(s)	453, 454, 786
Bme18I	GGWCC	2 cut(s)	48, 490
BmeT110I	CYCGRG	1 cut(s)	452
BmgT120I	GGNCC	3 cut(s)	48, 490, 828
BmiI	GGNNCC	2 cut(s)	659, 1322
BmrFI	CCNGG	3 cut(s)	453, 454, 786
BmrI	ACTGGG	2 cut(s)	902, 1079
BmsI	GCATC	3 cut(s)	796, 1276, 1279
BmuI	ACTGGG	2 cut(s)	902, 1079
BpiI	GAAGAC	1 cut(s)	360
BplI	GAGNNNNNCTC	2 cut(s)	1190, 1222
BpmI	CTGGAG	2 cut(s)	955, 1156
BpuEI	CTTGAG	3 cut(s)	73, 234, 1369
BpuMI	CCSGG	3 cut(s)	453, 454, 786
BsaBI	GATNNNNATC	1 cut(s)	1215
BsaJI	CCNNGG	4 cut(s)	44, 452, 453, 717
BsaWI	WCCGGW	1 cut(s)	660
Bsc4I	CCNNNNNNNGG	3 cut(s)	290, 587, 708
Bse1I	ACTGG	4 cut(s)	908, 1085, 1139, 1261
Bse8I	GATNNNNATC	1 cut(s)	1215
BseDI	CCNNGG	4 cut(s)	44, 452, 453, 717
BseGI	GGATG	2 cut(s)	105, 811
BseJI	GATNNNNATC	1 cut(s)	1215
BseLI	CCNNNNNNNGG	3 cut(s)	290, 587, 708
BseMII	CTCAG	2 cut(s)	53, 1161
BseNI	ACTGG	4 cut(s)	908, 1085, 1139, 1261
BseRI	GAGGAG	1 cut(s)	92
BseXI	GCAGC	2 cut(s)	1017, 1152
Bsh1236I	CGCG	2 cut(s)	46, 467
BshFI	GGCC	2 cut(s)	5, 829
BsiHKAI	GWGCWC	1 cut(s)	250
BsiHKCI	CYCGRG	1 cut(s)	452
BsiSI	CCGG	4 cut(s)	453, 661, 785, 891
BslFI	GGGAC	1 cut(s)	1067
BslI	CCNNNNNNNGG	3 cut(s)	290, 587, 708
BsmAI	GTCTC	3 cut(s)	42, 49, 102
BsmBI	CGTCTC	1 cut(s)	102
BsmFI	GGGAC	1 cut(s)	1067
BsmI	GAATGC	1 cut(s)	881
BsnI	GGCC	2 cut(s)	5, 829
BsoBI	CYCGRG	1 cut(s)	452
Bsp1286I	GDGCHC	1 cut(s)	250
Bsp143I	GATC	5 cut(s)	293, 520, 535, 676, 1200
Bsp19I	CCATGG	1 cut(s)	717
BspACI	CCGC	8 cut(s)	20, 44, 46, 239, 533, 571, 709, 1222
BspANI	GGCC	2 cut(s)	5, 829
BspCNI	CTCAG	2 cut(s)	52, 1160
BspFNI	CGCG	2 cut(s)	46, 467
BspLI	GGNNCC	2 cut(s)	659, 1322
BspMI	ACCTGC	1 cut(s)	999
BspPI	GGATC	3 cut(s)	301, 515, 543
BspQI	GCTCTTC	1 cut(s)	772
BspTI	CTTAAG	2 cut(s)	1181, 1193
BsrI	ACTGG	4 cut(s)	908, 1085, 1139, 1261
BssECI	CCNNGG	4 cut(s)	44, 452, 453, 717
BssMI	GATC	5 cut(s)	293, 520, 535, 676, 1200
BssT1I	CCWWGG	1 cut(s)	717
Bst4CI	ACNGT	3 cut(s)	245, 1076, 1342
Bst6I	CTCTTC	3 cut(s)	116, 143, 772
BstAFI	CTTAAG	2 cut(s)	1181, 1193
BstAPI	GCANNNNNTGC	1 cut(s)	1014
BstDEI	CTNAG	5 cut(s)	39, 82, 528, 1147, 1368
BstDSI	CCRYGG	2 cut(s)	44, 717
BstF5I	GGATG	2 cut(s)	105, 811
BstFNI	CGCG	2 cut(s)	46, 467
BstHHI	GCGC	2 cut(s)	961, 1251
BstKTI	GATC	5 cut(s)	296, 523, 538, 679, 1203
BstMAI	GTCTC	3 cut(s)	42, 49, 102
BstMBI	GATC	5 cut(s)	293, 520, 535, 676, 1200
BstMWI	GCNNNNNNNGC	5 cut(s)	245, 771, 887, 1014, 1055
BstNSI	RCATGY	1 cut(s)	813
BstSCI	CCNGG	3 cut(s)	451, 452, 784
BstUI	CGCG	2 cut(s)	46, 467
BstV1I	GCAGC	2 cut(s)	1017, 1152
BstV2I	GAAGAC	1 cut(s)	360
BstX2I	RGATCY	1 cut(s)	535
BstXI	CCANNNNNNTGG	1 cut(s)	825
BstYI	RGATCY	1 cut(s)	535
BsuI	GTATCC	1 cut(s)	611
BsuRI	GGCC	2 cut(s)	5, 829
BtgI	CCRYGG	2 cut(s)	44, 717
BtsCI	GGATG	2 cut(s)	105, 811
BtsI	GCAGTG	1 cut(s)	1175
BtsIMutI	CAGTG	3 cut(s)	1092, 1175, 1268
BveI	ACCTGC	1 cut(s)	999
CaiI	CAGNNNCTG	1 cut(s)	1014
CfoI	GCGC	2 cut(s)	961, 1251
Cfr13I	GGNCC	3 cut(s)	48, 490, 828
Cfr42I	CCGCGG	1 cut(s)	47
Cfr9I	CCCGGG	1 cut(s)	452
Csp6I	GTAC	3 cut(s)	71, 646, 911
CviAII	CATG	7 cut(s)	158, 332, 575, 718, 810, 1022, 1300
CviQI	GTAC	3 cut(s)	71, 646, 911
DdeI	CTNAG	5 cut(s)	39, 82, 528, 1147, 1368
DpnI	GATC	5 cut(s)	295, 522, 537, 678, 1202
DpnII	GATC	5 cut(s)	293, 520, 535, 676, 1200
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	3 cut(s)	116, 143, 772
EarI	CTCTTC	3 cut(s)	116, 143, 772
EciI	GGCGGA	1 cut(s)	548
Eco130I	CCWWGG	1 cut(s)	717
Eco32I	GATATC	1 cut(s)	1171
Eco47I	GGWCC	2 cut(s)	48, 490
Eco88I	CYCGRG	1 cut(s)	452
EcoO109I	RGGNCCY	1 cut(s)	490
EcoRV	GATATC	1 cut(s)	1171
EcoT14I	CCWWGG	1 cut(s)	717
EcoT22I	ATGCAT	1 cut(s)	811
ErhI	CCWWGG	1 cut(s)	717
Esp3I	CGTCTC	1 cut(s)	102
FaeI	CATG	7 cut(s)	161, 335, 578, 721, 813, 1025, 1303
FaqI	GGGAC	1 cut(s)	1067
FatI	CATG	7 cut(s)	157, 331, 574, 717, 809, 1021, 1299
FauNDI	CATATG	1 cut(s)	985
Fnu4HI	GCNGC	4 cut(s)	44, 572, 1006, 1141
FokI	GGATG	2 cut(s)	92, 818
Fsp4HI	GCNGC	4 cut(s)	44, 572, 1006, 1141
FspBI	CTAG	1 cut(s)	1166
FspI	TGCGCA	2 cut(s)	960, 1250
GlaI	GCGC	2 cut(s)	960, 1250
GluI	GCNGC	4 cut(s)	44, 572, 1006, 1141
GsuI	CTGGAG	2 cut(s)	955, 1156
HaeIII	GGCC	2 cut(s)	5, 829
HapII	CCGG	4 cut(s)	453, 661, 785, 891
HhaI	GCGC	2 cut(s)	961, 1251
Hin1II	CATG	7 cut(s)	161, 335, 578, 721, 813, 1025, 1303
Hin6I	GCGC	2 cut(s)	959, 1249
HinP1I	GCGC	2 cut(s)	959, 1249
HincII	GTYRAC	1 cut(s)	266
HindII	GTYRAC	1 cut(s)	266
HindIII	AAGCTT	1 cut(s)	920
HinfI	GANTC	7 cut(s)	8, 216, 413, 443, 897, 944, 1061
HpaII	CCGG	4 cut(s)	453, 661, 785, 891
HphI	GGTGA	1 cut(s)	264
Hpy166II	GTNNAC	1 cut(s)	266
Hpy188I	TCNGA	5 cut(s)	234, 448, 949, 1210, 1325
Hpy188III	TCNNGA	4 cut(s)	350, 484, 934, 1166
Hpy8I	GTNNAC	1 cut(s)	266
Hpy99I	CGWCG	1 cut(s)	1329
HpyAV	CCTTC	3 cut(s)	221, 481, 863
HpyCH4III	ACNGT	3 cut(s)	245, 1076, 1342
HpyCH4IV	ACGT	2 cut(s)	95, 687
HpyCH4V	TGCA	6 cut(s)	422, 428, 563, 809, 881, 1058
HpyF10VI	GCNNNNNNNGC	5 cut(s)	245, 771, 887, 1014, 1055
HpyF3I	CTNAG	5 cut(s)	39, 82, 528, 1147, 1368
HpySE526I	ACGT	2 cut(s)	95, 687
Hsp92II	CATG	7 cut(s)	161, 335, 578, 721, 813, 1025, 1303
HspAI	GCGC	2 cut(s)	959, 1249
KspI	CCGCGG	1 cut(s)	47
Kzo9I	GATC	5 cut(s)	293, 520, 535, 676, 1200
LguI	GCTCTTC	1 cut(s)	772
LmnI	GCTCC	5 cut(s)	375, 671, 1137, 1211, 1326
Lsp1109I	GCAGC	2 cut(s)	1017, 1152
LweI	GCATC	3 cut(s)	796, 1276, 1279
MaeI	CTAG	1 cut(s)	1166
MaeII	ACGT	2 cut(s)	95, 687
MaeIII	GTNAC	4 cut(s)	91, 476, 638, 1224
MalI	GATC	5 cut(s)	295, 522, 537, 678, 1202
MboI	GATC	5 cut(s)	293, 520, 535, 676, 1200
MfeI	CAATTG	2 cut(s)	287, 558
MflI	RGATCY	1 cut(s)	535
MhlI	GDGCHC	1 cut(s)	250
MluCI	AATT	9 cut(s)	287, 304, 460, 558, 585, 740, 822, 1092, 1354
MlyI	GAGTC	3 cut(s)	225, 407, 938
MmeI	TCCRAC	1 cut(s)	1348
Mph1103I	ATGCAT	1 cut(s)	811
MroXI	GAANNNNTTC	1 cut(s)	921
MseI	TTAA	4 cut(s)	381, 744, 1182, 1194
MslI	CAYNNNNRTG	3 cut(s)	156, 804, 1304
MspA1I	CMGCKG	1 cut(s)	46
MspCI	CTTAAG	2 cut(s)	1181, 1193
MspI	CCGG	4 cut(s)	453, 661, 785, 891
MspR9I	CCNGG	3 cut(s)	453, 454, 786
MunI	CAATTG	2 cut(s)	287, 558
Mva1269I	GAATGC	1 cut(s)	881
MvnI	CGCG	2 cut(s)	46, 467
MwoI	GCNNNNNNNGC	5 cut(s)	245, 771, 887, 1014, 1055
NciI	CCSGG	3 cut(s)	453, 454, 786
NcoI	CCATGG	1 cut(s)	717
NdeI	CATATG	1 cut(s)	985
NdeII	GATC	5 cut(s)	293, 520, 535, 676, 1200
NlaIII	CATG	7 cut(s)	161, 335, 578, 721, 813, 1025, 1303
NlaIV	GGNNCC	2 cut(s)	659, 1322
NmuCI	GTSAC	1 cut(s)	638
NsbI	TGCGCA	2 cut(s)	960, 1250
NsiI	ATGCAT	1 cut(s)	811
NspI	RCATGY	1 cut(s)	813
PciSI	GCTCTTC	1 cut(s)	772
PctI	GAATGC	1 cut(s)	881
PdmI	GAANNNNTTC	1 cut(s)	921
PfeI	GAWTC	4 cut(s)	8, 443, 897, 1061
PkrI	GCNGC	4 cut(s)	45, 573, 1007, 1142
PleI	GAGTC	3 cut(s)	224, 407, 938
PpsI	GAGTC	3 cut(s)	224, 407, 938
PpuMI	RGGWCCY	1 cut(s)	490
PsiI	TTATAA	1 cut(s)	614
Psp5II	RGGWCCY	1 cut(s)	490
PspN4I	GGNNCC	2 cut(s)	659, 1322
PspPI	GGNCC	3 cut(s)	48, 490, 828
PspPPI	RGGWCCY	1 cut(s)	490
PstNI	CAGNNNCTG	1 cut(s)	1014
PsuI	RGATCY	1 cut(s)	535
RsaI	GTAC	3 cut(s)	72, 647, 912
RsaNI	GTAC	3 cut(s)	71, 646, 911
RseI	CAYNNNNRTG	3 cut(s)	156, 804, 1304
SacII	CCGCGG	1 cut(s)	47
SapI	GCTCTTC	1 cut(s)	772
SaqAI	TTAA	4 cut(s)	381, 744, 1182, 1194
SatI	GCNGC	4 cut(s)	44, 572, 1006, 1141
Sau3AI	GATC	5 cut(s)	293, 520, 535, 676, 1200
Sau96I	GGNCC	3 cut(s)	48, 490, 828
SchI	GAGTC	3 cut(s)	225, 407, 938
ScrFI	CCNGG	3 cut(s)	453, 454, 786
SduI	GDGCHC	1 cut(s)	250
SfaNI	GCATC	3 cut(s)	796, 1276, 1279
Sfr303I	CCGCGG	1 cut(s)	47
SgrBI	CCGCGG	1 cut(s)	47
SinI	GGWCC	2 cut(s)	48, 490
SmaI	CCCGGG	1 cut(s)	454
SmiMI	CAYNNNNRTG	3 cut(s)	156, 804, 1304
SmlI	CTYRAG	5 cut(s)	52, 249, 1181, 1193, 1348
SmoI	CTYRAG	5 cut(s)	52, 249, 1181, 1193, 1348
Sse9I	AATT	9 cut(s)	287, 304, 460, 558, 585, 740, 822, 1092, 1354
SsiI	CCGC	8 cut(s)	20, 44, 46, 239, 533, 571, 709, 1222
SspI	AATATT	2 cut(s)	190, 653
SspMI	CTAG	1 cut(s)	1166
StyD4I	CCNGG	3 cut(s)	451, 452, 784
StyI	CCWWGG	1 cut(s)	717
TaaI	ACNGT	3 cut(s)	245, 1076, 1342
TaiI	ACGT	2 cut(s)	98, 690
TaqI	TCGA	5 cut(s)	214, 219, 258, 296, 1337
TasI	AATT	9 cut(s)	287, 304, 460, 558, 585, 740, 822, 1092, 1354
TauI	GCSGC	2 cut(s)	46, 574
TfiI	GAWTC	4 cut(s)	8, 443, 897, 1061
Tru1I	TTAA	4 cut(s)	381, 744, 1182, 1194
Tru9I	TTAA	4 cut(s)	381, 744, 1182, 1194
TscAI	CASTG	3 cut(s)	1092, 1182, 1268
TseFI	GTSAC	1 cut(s)	638
TseI	GCWGC	2 cut(s)	1005, 1140
Tsp45I	GTSAC	1 cut(s)	638
TspDTI	ATGAA	8 cut(s)	183, 383, 499, 512, 747, 852, 1008, 1227
TspMI	CCCGGG	1 cut(s)	452
TspRI	CASTG	3 cut(s)	1092, 1182, 1268
Vha464I	CTTAAG	2 cut(s)	1181, 1193
VpaK11BI	GGWCC	2 cut(s)	48, 490
XapI	RAATTY	2 cut(s)	740, 822
XbaI	TCTAGA	1 cut(s)	1165
XceI	RCATGY	1 cut(s)	813
XcmI	CCANNNNNNNNNTGG	1 cut(s)	589
XmaI	CCCGGG	1 cut(s)	452
XmnI	GAANNNNTTC	1 cut(s)	921
XspI	CTAG	1 cut(s)	1166
Zsp2I	ATGCAT	1 cut(s)	811

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.