Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0017739

Physical Location & Seq

Genomic Coordinates Forward (+)

815654 .. 817117

Gene Structure 1464 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1007300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 966 bp

ATGCTGGCCATGTCGATCCTCAATTTTGAAACTGAATGCAGAGAGATTCATGGTTCTTGGGGACAGCTAAACCGATTGGCTCGAGCTTTGGCGAATCGAACACGGCATGAGAGGCAACAGATTAGAGAAACTTACAAGTCAATCTATGGGGATGACTTGGTAAACAGACTCAAAGATGCAGCTGCTGCTGGGATTTCACCAAAATTATGTGCAGCCTTGTCTATGTGGATGACTGAGCAGCACGAGCGTGATGCTGTCGTGGTCCGCGAGGCCCTTGATGATCAGAAAGGTGATACAAACTACAACGCGCTTGTAGAGATTTTTGTGGGTCGAAAATCTAGTCATATCCTTCTTATCAAGCAAGCATACTACAGAAGATTTTGGAGGCAGTTAGACCAAGATATCATCAACATCGAGCCTCCCAATCCTTACCAAAAGATACTGGTAGCATTGGTTGCATCACATAAGGCACACCATGCGGACGTTAGCCAACATATCGCGAAGGCGGATGCAAGGAGGCTTTACGAAACTGGGGAGGGGAGTTCCGGTCCCATAGAAGAAGCCGTTGTCTTAGAAATACTAAGTAAACGGAGTATTCCGCAGCTTAAGCTTACATTTTCATGCTATAAACACATCTACGGACATGAGTATACAGAGTCCCTGAAAACCAGAAACTACGGAGAACTTGAAAATGCAATGAAAATGATAGTACAATGCATCTACAATCCACCTGACTATTTCGCTATGATGTTGTATGCAAGCATGAAGGGGTGCATCAGCGACGAAGGTGCAGTGCGACGTGTGGTGGTGAGCAGGGCAGAAGTTGACATGGATGAAATTAAAAGGGAGTTTAAGAAGAAGCATGGGATGGAACTGAGAGATGCAATCTGTGACAACATCCCTTCCGGCGACTATAGAGACTTTCTTGTTGCTTTGACGGTCTCGAAATCAAATGAATCTGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0010035 GO:0042221 GO:0050896 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Annexin

Protein Analysis

322

Amino Acids

36.73

Weight (kDa)

7.17

Isoelectric Point (pI)

45.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Annexin	PF00191	25 - 60	8.7e-06	Annexin
Annexin	PF00191	170 - 235	3.9e-07	Annexin
Annexin	PF00191	246 - 312	3e-12	Annexin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017739)

Species	Orthologous Gene IDs
malus_domestica	MD12G1007300.v1.1
prunus_persica	Prupe.7G008200_v2.0.a1
pyrus_communis	pycom12g00530
rosa_chinensis	RchiOBHm_Chr3g0496581
rosa_laevigata	RLG00000022517
rosa_roxburghii	Rroxscaffold_6G00388900
rosa_rugosa	Rorug03G0284700
rosa_samantha	Rh3AG329900 Rh3BG365700 Rh3CG362700 Rh3DG365900
rosa_wichuraiana	Rw3G028870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	650
AccII	CGCG	3 cut(s)	267, 308, 500
AciI	CCGC	4 cut(s)	265, 479, 506, 599
AclWI	GGATC	1 cut(s)	10
AcoI	YGGCCR	1 cut(s)	6
AfaI	GTAC	1 cut(s)	711
AflII	CTTAAG	1 cut(s)	605
AflIII	ACRYGT	1 cut(s)	799
AgsI	TTSAA	2 cut(s)	29, 689
AjiI	CACGTC	1 cut(s)	800
AleI	CACNNNNGTG	1 cut(s)	246
AluBI	AGCT	5 cut(s)	67, 86, 182, 604, 610
AluI	AGCT	5 cut(s)	67, 86, 182, 604, 610
Alw26I	GTCTC	2 cut(s)	912, 946
AlwI	GGATC	1 cut(s)	10
AlwNI	CAGNNNCTG	1 cut(s)	185
Ama87I	CYCGRG	1 cut(s)	81
AoxI	GGCC	2 cut(s)	6, 270
ApeKI	GCWGC	6 cut(s)	179, 182, 185, 212, 238, 601
ArsI	GACNNNNNNTTYG	2 cut(s)	325, 357
AspLEI	GCGC	1 cut(s)	310
AspS9I	GGNCC	3 cut(s)	262, 271, 548
AsuHPI	GGTGA	3 cut(s)	189, 302, 820
AvaI	CYCGRG	1 cut(s)	81
AvaII	GGWCC	2 cut(s)	262, 548
BalI	TGGCCA	1 cut(s)	8
BauI	CACGAG	1 cut(s)	242
BbvI	GCAGC	6 cut(s)	169, 172, 191, 224, 250, 613
BccI	CCATC	1 cut(s)	862
BceAI	ACGGC	2 cut(s)	119, 548
BcgI	CGANNNNNNTGC	4 cut(s)	233, 267, 770, 804
BclI	TGATCA	1 cut(s)	280
BcoDI	GTCTC	2 cut(s)	912, 946
BfaI	CTAG	1 cut(s)	339
BfmI	CTRYAG	2 cut(s)	370, 913
BfrI	CTTAAG	1 cut(s)	605
BisI	GCNGC	6 cut(s)	180, 183, 186, 213, 239, 602
BlsI	GCNGC	6 cut(s)	181, 184, 187, 214, 240, 603
Bme18I	GGWCC	2 cut(s)	262, 548
BmeT110I	CYCGRG	1 cut(s)	81
BmgBI	CACGTC	1 cut(s)	800
BmgT120I	GGNCC	3 cut(s)	262, 271, 548
BmiI	GGNNCC	1 cut(s)	550
BmrI	ACTGGG	1 cut(s)	540
BmsI	GCATC	7 cut(s)	166, 241, 467, 499, 726, 783, 871
BmuI	ACTGGG	1 cut(s)	540
BsaI	GGTCTC	1 cut(s)	946
BsaWI	WCCGGW	1 cut(s)	545
BsaXI	ACNNNNNCTCC	2 cut(s)	532, 562
Bse1I	ACTGG	2 cut(s)	447, 535
Bse3DI	GCAATG	1 cut(s)	702
BseGI	GGATG	6 cut(s)	157, 234, 514, 838, 873, 897
BseMI	GCAATG	1 cut(s)	702
BseMII	CTCAG	2 cut(s)	225, 866
BseNI	ACTGG	2 cut(s)	447, 535
BseXI	GCAGC	6 cut(s)	169, 172, 191, 224, 250, 613
BseYI	CCCAGC	1 cut(s)	188
BsgI	GTGCAG	2 cut(s)	231, 810
Bsh1236I	CGCG	3 cut(s)	267, 308, 500
BshFI	GGCC	2 cut(s)	8, 272
BsiHKCI	CYCGRG	1 cut(s)	81
BsiSI	CCGG	2 cut(s)	546, 906
BslFI	GGGAC	3 cut(s)	75, 534, 643
BsmAI	GTCTC	2 cut(s)	912, 946
BsmFI	GGGAC	3 cut(s)	75, 534, 643
BsmI	GAATGC	1 cut(s)	41
BsnI	GGCC	2 cut(s)	8, 272
Bso31I	GGTCTC	1 cut(s)	946
BsoBI	CYCGRG	1 cut(s)	81
Bsp143I	GATC	2 cut(s)	15, 280
Bsp68I	TCGCGA	1 cut(s)	500
BspACI	CCGC	4 cut(s)	265, 479, 506, 599
BspANI	GGCC	2 cut(s)	8, 272
BspCNI	CTCAG	2 cut(s)	226, 867
BspFNI	CGCG	3 cut(s)	267, 308, 500
BspLI	GGNNCC	1 cut(s)	550
BspPI	GGATC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	605
BspTNI	GGTCTC	1 cut(s)	946
BsrDI	GCAATG	1 cut(s)	702
BsrI	ACTGG	2 cut(s)	447, 535
BssMI	GATC	2 cut(s)	15, 280
BssNAI	GTATAC	1 cut(s)	651
BssSI	CACGAG	1 cut(s)	242
Bst1107I	GTATAC	1 cut(s)	651
Bst2BI	CACGAG	1 cut(s)	242
Bst4CI	ACNGT	1 cut(s)	940
BstAFI	CTTAAG	1 cut(s)	605
BstAPI	GCANNNNNTGC	3 cut(s)	185, 455, 476
BstC8I	GCNNGC	3 cut(s)	6, 363, 760
BstDEI	CTNAG	4 cut(s)	234, 571, 581, 875
BstF5I	GGATG	6 cut(s)	157, 234, 514, 838, 873, 897
BstFNI	CGCG	3 cut(s)	267, 308, 500
BstHHI	GCGC	1 cut(s)	310
BstKTI	GATC	2 cut(s)	18, 283
BstMAI	GTCTC	2 cut(s)	912, 946
BstMBI	GATC	2 cut(s)	15, 280
BstMWI	GCNNNNNNNGC	6 cut(s)	112, 185, 244, 455, 476, 607
BstSFI	CTRYAG	2 cut(s)	370, 913
BstUI	CGCG	3 cut(s)	267, 308, 500
BstV1I	GCAGC	6 cut(s)	169, 172, 191, 224, 250, 613
BstZ17I	GTATAC	1 cut(s)	651
BsuRI	GGCC	2 cut(s)	8, 272
BtrI	CACGTC	1 cut(s)	800
BtsCI	GGATG	6 cut(s)	157, 234, 514, 838, 873, 897
BtsI	GCAGTG	1 cut(s)	798
BtsIMutI	CAGTG	1 cut(s)	798
BtuMI	TCGCGA	1 cut(s)	500
Cac8I	GCNNGC	3 cut(s)	6, 363, 760
CaiI	CAGNNNCTG	1 cut(s)	185
CfoI	GCGC	1 cut(s)	310
Cfr13I	GGNCC	3 cut(s)	262, 271, 548
Csp6I	GTAC	1 cut(s)	710
CviAII	CATG	9 cut(s)	10, 50, 107, 476, 621, 644, 763, 829, 863
CviQI	GTAC	1 cut(s)	710
DdeI	CTNAG	4 cut(s)	234, 571, 581, 875
DpnI	GATC	2 cut(s)	17, 282
DpnII	GATC	2 cut(s)	15, 280
EaeI	YGGCCR	1 cut(s)	6
EciI	GGCGGA	1 cut(s)	521
Eco31I	GGTCTC	1 cut(s)	946
Eco32I	GATATC	1 cut(s)	403
Eco47I	GGWCC	2 cut(s)	262, 548
Eco88I	CYCGRG	1 cut(s)	81
EcoO109I	RGGNCCY	1 cut(s)	271
EcoRV	GATATC	1 cut(s)	403
EcoT22I	ATGCAT	1 cut(s)	719
FaeI	CATG	9 cut(s)	13, 53, 110, 479, 624, 647, 766, 832, 866
FaqI	GGGAC	3 cut(s)	75, 534, 643
FatI	CATG	9 cut(s)	9, 49, 106, 475, 620, 643, 762, 828, 862
FbaI	TGATCA	1 cut(s)	280
FblI	GTMKAC	1 cut(s)	650
Fnu4HI	GCNGC	6 cut(s)	180, 183, 186, 213, 239, 602
FokI	GGATG	6 cut(s)	164, 241, 521, 845, 880, 884
Fsp4HI	GCNGC	6 cut(s)	180, 183, 186, 213, 239, 602
FspBI	CTAG	1 cut(s)	339
GlaI	GCGC	1 cut(s)	309
GluI	GCNGC	6 cut(s)	180, 183, 186, 213, 239, 602
GsaI	CCCAGC	1 cut(s)	192
HaeIII	GGCC	2 cut(s)	8, 272
HapII	CCGG	2 cut(s)	546, 906
HhaI	GCGC	1 cut(s)	310
Hin1II	CATG	9 cut(s)	13, 53, 110, 479, 624, 647, 766, 832, 866
Hin6I	GCGC	1 cut(s)	308
HinP1I	GCGC	1 cut(s)	308
HincII	GTYRAC	1 cut(s)	826
HindII	GTYRAC	1 cut(s)	826
HindIII	AAGCTT	1 cut(s)	608
HinfI	GANTC	5 cut(s)	46, 94, 168, 656, 956
HpaII	CCGG	2 cut(s)	546, 906
HphI	GGTGA	3 cut(s)	189, 302, 820
Hpy166II	GTNNAC	4 cut(s)	163, 587, 651, 826
Hpy188I	TCNGA	1 cut(s)	285
Hpy188III	TCNNGA	2 cut(s)	499, 943
Hpy8I	GTNNAC	4 cut(s)	163, 587, 651, 826
Hpy99I	CGWCG	2 cut(s)	785, 801
HpyAV	CCTTC	5 cut(s)	359, 496, 760, 779, 912
HpyCH4III	ACNGT	1 cut(s)	940
HpyCH4IV	ACGT	2 cut(s)	483, 799
HpyF10VI	GCNNNNNNNGC	6 cut(s)	112, 185, 244, 455, 476, 607
HpyF3I	CTNAG	4 cut(s)	234, 571, 581, 875
HpySE526I	ACGT	2 cut(s)	483, 799
Hsp92II	CATG	9 cut(s)	13, 53, 110, 479, 624, 647, 766, 832, 866
HspAI	GCGC	1 cut(s)	308
Ksp22I	TGATCA	1 cut(s)	280
Kzo9I	GATC	2 cut(s)	15, 280
LpnPI	CCDG	9 cut(s)	174, 428, 516, 559, 674, 682, 744, 799, 919
Lsp1109I	GCAGC	6 cut(s)	169, 172, 191, 224, 250, 613
LweI	GCATC	7 cut(s)	166, 241, 467, 499, 726, 783, 871
MaeI	CTAG	1 cut(s)	339
MaeII	ACGT	2 cut(s)	483, 799
MaeIII	GTNAC	1 cut(s)	890
MalI	GATC	2 cut(s)	17, 282
MboI	GATC	2 cut(s)	15, 280
MboII	GAAGA	3 cut(s)	387, 569, 868
MlsI	TGGCCA	1 cut(s)	8
MluCI	AATT	3 cut(s)	22, 203, 837
MluNI	TGGCCA	1 cut(s)	8
MlyI	GAGTC	2 cut(s)	162, 665
MnlI	CCTC	7 cut(s)	29, 105, 262, 378, 429, 510, 529
Mox20I	TGGCCA	1 cut(s)	8
Mph1103I	ATGCAT	1 cut(s)	719
MscI	TGGCCA	1 cut(s)	8
MseI	TTAA	3 cut(s)	606, 840, 852
MslI	CAYNNNNRTG	2 cut(s)	246, 619
Msp20I	TGGCCA	1 cut(s)	8
MspA1I	CMGCKG	1 cut(s)	182
MspCI	CTTAAG	1 cut(s)	605
MspI	CCGG	2 cut(s)	546, 906
Mva1269I	GAATGC	1 cut(s)	41
MvnI	CGCG	3 cut(s)	267, 308, 500
MwoI	GCNNNNNNNGC	6 cut(s)	112, 185, 244, 455, 476, 607
NdeII	GATC	2 cut(s)	15, 280
NlaIII	CATG	9 cut(s)	13, 53, 110, 479, 624, 647, 766, 832, 866
NlaIV	GGNNCC	1 cut(s)	550
NmuCI	GTSAC	1 cut(s)	890
NruI	TCGCGA	1 cut(s)	500
NsiI	ATGCAT	1 cut(s)	719
OliI	CACNNNNGTG	1 cut(s)	246
PaeR7I	CTCGAG	1 cut(s)	81
PcsI	WCGNNNNNNNCGW	1 cut(s)	264
PctI	GAATGC	1 cut(s)	41
PfeI	GAWTC	3 cut(s)	46, 94, 956
PkrI	GCNGC	6 cut(s)	181, 184, 187, 214, 240, 603
PleI	GAGTC	2 cut(s)	162, 664
PpsI	GAGTC	2 cut(s)	162, 664
PspFI	CCCAGC	1 cut(s)	188
PspN4I	GGNNCC	1 cut(s)	550
PspPI	GGNCC	3 cut(s)	262, 271, 548
PspXI	VCTCGAGB	1 cut(s)	81
PstNI	CAGNNNCTG	1 cut(s)	185
PvuII	CAGCTG	1 cut(s)	182
RruI	TCGCGA	1 cut(s)	500
RsaI	GTAC	1 cut(s)	711
RsaNI	GTAC	1 cut(s)	710
RseI	CAYNNNNRTG	2 cut(s)	246, 619
SaqAI	TTAA	3 cut(s)	606, 840, 852
SatI	GCNGC	6 cut(s)	180, 183, 186, 213, 239, 602
Sau3AI	GATC	2 cut(s)	15, 280
Sau96I	GGNCC	3 cut(s)	262, 271, 548
SchI	GAGTC	2 cut(s)	162, 665
SfaNI	GCATC	7 cut(s)	166, 241, 467, 499, 726, 783, 871
SfcI	CTRYAG	2 cut(s)	370, 913
Sfr274I	CTCGAG	1 cut(s)	81
SinI	GGWCC	2 cut(s)	262, 548
SlaI	CTCGAG	1 cut(s)	81
SmiMI	CAYNNNNRTG	2 cut(s)	246, 619
SmlI	CTYRAG	2 cut(s)	81, 605
SmoI	CTYRAG	2 cut(s)	81, 605
Sse9I	AATT	3 cut(s)	22, 203, 837
SsiI	CCGC	4 cut(s)	265, 479, 506, 599
SspMI	CTAG	1 cut(s)	339
TaaI	ACNGT	1 cut(s)	940
TaiI	ACGT	2 cut(s)	486, 802
TaqI	TCGA	6 cut(s)	14, 82, 97, 331, 414, 944
TasI	AATT	3 cut(s)	22, 203, 837
TatI	WGTACW	1 cut(s)	709
TfiI	GAWTC	3 cut(s)	46, 94, 956
Tru1I	TTAA	3 cut(s)	606, 840, 852
Tru9I	TTAA	3 cut(s)	606, 840, 852
TscAI	CASTG	1 cut(s)	798
TseFI	GTSAC	1 cut(s)	890
TseI	GCWGC	6 cut(s)	179, 182, 185, 212, 238, 601
Tsp45I	GTSAC	1 cut(s)	890
TspDTI	ATGAA	5 cut(s)	38, 609, 713, 779, 849
TspGWI	ACGGA	3 cut(s)	604, 654, 693
TspRI	CASTG	1 cut(s)	798
Vha464I	CTTAAG	1 cut(s)	605
VpaK11BI	GGWCC	2 cut(s)	262, 548
XhoI	CTCGAG	1 cut(s)	81
XmiI	GTMKAC	1 cut(s)	650
XspI	CTAG	1 cut(s)	339
Zsp2I	ATGCAT	1 cut(s)	719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.