pycom12g00530

Annexin A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0017739
Physical Location & Seq
Forward (+)
500186 .. 501587
1402 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00530.2

Sequence Viewer

Length: 1041 bp
ATGAATAAATACACTGCTATCCAGCACCACCACTTAACAAAATTGTCTCTGCACTTCAATAACCAATCAAAAATAATGCTGGCCATGTCGACCCTCAGTTTTGAAACTGAATGCAGAGAGATTCATGTTTCTTGGGGACAGCTAAACCGATTGGCTCGAGCTTTGGCAAATCGAACGCGGCATGAGAGGCAACAGATTAGAGAAACTTACAAGTCAATCTATGGAGATGACTTGGTAAACAGACTCAAAGAAGCAGCTGCTGCTGGGATTTCACCAAAATTATGTGCTGCCTTGTCTATGTGGATGACTGAGCAGCACGAGCGTGATGCTGTCGTGGTCCGCGAGGCCCTTGATGATCAGAAAGGCAATACAAACTACAACGCGCTTGTAGAGATTTTTGTGGGTCGAAAATCGAGTCATATCCTTCTGATCAAGCAAGCATACTACAGAAGATTTTGGAGGCAGTTAGACCAAGATATCATCAACATCGAGCCTCCCAATCCTTACCAAAAGATACTGGTAGCATTGGTTGCATCACATAAGGCACACCATGCGGACGTTAGCCAACATATCGCGAAGGTGGACGCAAGGAGGCTTTACGAAACTGGGGAGGGGGGTTCCGGTCCCATAGAAGAAGCCGTTGTCTTAGAAATACTAAGTAAACGGAGTATTCCGCAGCTTAAGCTTACATTTTCGTGCTATAAACACATCTACGGACATGAGTATACAGAGTCCCTGAAAACCAGAAACTACGGAGAACTTGAAAATGCAATGAAAATGGTAGTACAATGCATCTACAATCCACCTGACTATTTCGCTACGATGTTGTATGCAAGCATGAAGGGGTGTATCAGCGACGAAGGTGCAGTGCGACGTGTGATGGTGAGCAGGGCAGAAGTAGACATGGATGAAATTAAAAGGGAGTTTAAGAAGAAGCATGGAATGGAACTGAGAGATGCAATCTGTGACAACATCCCGTCCGGCGACTATAGAGAATTTCTTGTTGCTTTGACGGTCTCGAAATCAAATGAATCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0010035 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Annexin

Protein Analysis

347

Amino Acids

39.73

Weight (kDa)

8.58

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017739)

Species Orthologous Gene IDs
malus_domestica MD12G1007300.v1.1
prunus_persica Prupe.7G008200_v2.0.a1
pyrus_communis pycom12g00530
rosa_chinensis RchiOBHm_Chr3g0496581
rosa_laevigata RLG00000022517
rosa_roxburghii Rroxscaffold_6G00388900
rosa_rugosa Rorug03G0284700
rosa_samantha Rh3AG329900 Rh3BG365700 Rh3CG362700 Rh3DG365900
rosa_wichuraiana Rw3G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 89, 725, 900
AccII CGCG 4 cut(s) 178, 342, 383, 575
AciI CCGC 4 cut(s) 178, 340, 554, 674
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 995
AfaI GTAC 1 cut(s) 786
AflII CTTAAG 1 cut(s) 680
AflIII ACRYGT 1 cut(s) 874
AgsI TTSAA 3 cut(s) 58, 104, 764
AjiI CACGTC 1 cut(s) 875
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 5 cut(s) 142, 161, 257, 679, 685
AluI AGCT 5 cut(s) 142, 161, 257, 679, 685
Alw26I GTCTC 2 cut(s) 51, 1021
AlwNI CAGNNNCTG 1 cut(s) 260
Ama87I CYCGRG 1 cut(s) 156
AoxI GGCC 2 cut(s) 81, 345
ApeKI GCWGC 6 cut(s) 254, 257, 260, 287, 313, 676
ApoI RAATTY 1 cut(s) 995
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 3 cut(s) 337, 346, 623
AsuHPI GGTGA 2 cut(s) 264, 895
AvaI CYCGRG 1 cut(s) 156
AvaII GGWCC 2 cut(s) 337, 623
BalI TGGCCA 1 cut(s) 83
BauI CACGAG 1 cut(s) 317
BbvI GCAGC 6 cut(s) 244, 247, 266, 274, 325, 688
BccI CCATC 1 cut(s) 874
BceAI ACGGC 1 cut(s) 623
BcgI CGANNNNNNTGC 4 cut(s) 308, 342, 845, 879
BclI TGATCA 2 cut(s) 355, 429
BcoDI GTCTC 2 cut(s) 51, 1021
BfmI CTRYAG 2 cut(s) 445, 988
BfrI CTTAAG 1 cut(s) 680
BisI GCNGC 7 cut(s) 179, 255, 258, 261, 288, 314, 677
BlsI GCNGC 7 cut(s) 180, 256, 259, 262, 289, 315, 678
Bme18I GGWCC 2 cut(s) 337, 623
BmeT110I CYCGRG 1 cut(s) 156
BmgBI CACGTC 1 cut(s) 875
BmgT120I GGNCC 3 cut(s) 337, 346, 623
BmiI GGNNCC 2 cut(s) 619, 625
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 4 cut(s) 316, 542, 801, 946
BmuI ACTGGG 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 1021
BsaWI WCCGGW 1 cut(s) 620
Bse1I ACTGG 2 cut(s) 522, 610
Bse3DI GCAATG 1 cut(s) 777
BseGI GGATG 3 cut(s) 309, 913, 972
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 3 cut(s) 109, 300, 941
BseNI ACTGG 2 cut(s) 522, 610
BseXI GCAGC 6 cut(s) 244, 247, 266, 274, 325, 688
BseYI CCCAGC 1 cut(s) 263
BsgI GTGCAG 2 cut(s) 35, 885
Bsh1236I CGCG 4 cut(s) 178, 342, 383, 575
BshFI GGCC 2 cut(s) 83, 347
BsiHKCI CYCGRG 1 cut(s) 156
BsiSI CCGG 2 cut(s) 621, 981
BslFI GGGAC 3 cut(s) 150, 609, 718
BsmAI GTCTC 2 cut(s) 51, 1021
BsmFI GGGAC 3 cut(s) 150, 609, 718
BsmI GAATGC 1 cut(s) 116
BsnI GGCC 2 cut(s) 83, 347
Bso31I GGTCTC 1 cut(s) 1021
BsoBI CYCGRG 1 cut(s) 156
Bsp143I GATC 2 cut(s) 355, 429
Bsp68I TCGCGA 1 cut(s) 575
BspACI CCGC 4 cut(s) 178, 340, 554, 674
BspANI GGCC 2 cut(s) 83, 347
BspCNI CTCAG 3 cut(s) 108, 301, 942
BspFNI CGCG 4 cut(s) 178, 342, 383, 575
BspLI GGNNCC 2 cut(s) 619, 625
BspTI CTTAAG 1 cut(s) 680
BspTNI GGTCTC 1 cut(s) 1021
BsrDI GCAATG 1 cut(s) 777
BsrI ACTGG 2 cut(s) 522, 610
BssMI GATC 2 cut(s) 355, 429
BssNAI GTATAC 1 cut(s) 726
BssSI CACGAG 1 cut(s) 317
Bst1107I GTATAC 1 cut(s) 726
Bst2BI CACGAG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 1015
BstAFI CTTAAG 1 cut(s) 680
BstAPI GCANNNNNTGC 3 cut(s) 260, 530, 551
BstC8I GCNNGC 3 cut(s) 81, 438, 835
BstDEI CTNAG 5 cut(s) 95, 309, 646, 656, 950
BstF5I GGATG 3 cut(s) 309, 913, 972
BstFNI CGCG 4 cut(s) 178, 342, 383, 575
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 2 cut(s) 358, 432
BstMAI GTCTC 2 cut(s) 51, 1021
BstMBI GATC 2 cut(s) 355, 429
BstMWI GCNNNNNNNGC 6 cut(s) 187, 260, 319, 530, 551, 682
BstSFI CTRYAG 2 cut(s) 445, 988
BstUI CGCG 4 cut(s) 178, 342, 383, 575
BstV1I GCAGC 6 cut(s) 244, 247, 266, 274, 325, 688
BstZ17I GTATAC 1 cut(s) 726
BsuRI GGCC 2 cut(s) 83, 347
BtrI CACGTC 1 cut(s) 875
BtsCI GGATG 3 cut(s) 309, 913, 972
BtsI GCAGTG 2 cut(s) 12, 873
BtsIMutI CAGTG 2 cut(s) 12, 873
BtuMI TCGCGA 1 cut(s) 575
Cac8I GCNNGC 3 cut(s) 81, 438, 835
CaiI CAGNNNCTG 1 cut(s) 260
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 337, 346, 623
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 785
CviAII CATG 8 cut(s) 85, 125, 182, 551, 719, 838, 904, 938
CviQI GTAC 1 cut(s) 785
DdeI CTNAG 5 cut(s) 95, 309, 646, 656, 950
DpnI GATC 2 cut(s) 357, 431
DpnII GATC 2 cut(s) 355, 429
EaeI YGGCCR 1 cut(s) 81
Eco31I GGTCTC 1 cut(s) 1021
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 2 cut(s) 337, 623
Eco88I CYCGRG 1 cut(s) 156
EcoO109I RGGNCCY 1 cut(s) 346
EcoRV GATATC 1 cut(s) 478
EcoT22I ATGCAT 1 cut(s) 794
FaeI CATG 8 cut(s) 88, 128, 185, 554, 722, 841, 907, 941
FaqI GGGAC 3 cut(s) 150, 609, 718
FatI CATG 8 cut(s) 84, 124, 181, 550, 718, 837, 903, 937
FbaI TGATCA 2 cut(s) 355, 429
FblI GTMKAC 3 cut(s) 89, 725, 900
Fnu4HI GCNGC 7 cut(s) 179, 255, 258, 261, 288, 314, 677
FokI GGATG 3 cut(s) 316, 920, 959
Fsp4HI GCNGC 7 cut(s) 179, 255, 258, 261, 288, 314, 677
GlaI GCGC 1 cut(s) 384
GluI GCNGC 7 cut(s) 179, 255, 258, 261, 288, 314, 677
GsaI CCCAGC 1 cut(s) 267
HaeIII GGCC 2 cut(s) 83, 347
HapII CCGG 2 cut(s) 621, 981
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 385
Hin1II CATG 8 cut(s) 88, 128, 185, 554, 722, 841, 907, 941
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 5 cut(s) 121, 243, 415, 731, 1031
HpaII CCGG 2 cut(s) 621, 981
HphI GGTGA 2 cut(s) 264, 895
Hpy166II GTNNAC 6 cut(s) 90, 238, 583, 662, 726, 901
Hpy188I TCNGA 2 cut(s) 360, 429
Hpy188III TCNNGA 2 cut(s) 574, 1018
Hpy8I GTNNAC 6 cut(s) 90, 238, 583, 662, 726, 901
Hpy99I CGWCG 2 cut(s) 860, 876
HpyAV CCTTC 4 cut(s) 434, 571, 835, 854
HpyCH4III ACNGT 1 cut(s) 1015
HpyCH4IV ACGT 2 cut(s) 558, 874
HpyCH4V TGCA 8 cut(s) 52, 114, 533, 770, 792, 833, 866, 959
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 260, 319, 530, 551, 682
HpyF3I CTNAG 5 cut(s) 95, 309, 646, 656, 950
HpySE526I ACGT 2 cut(s) 558, 874
Hsp92II CATG 8 cut(s) 88, 128, 185, 554, 722, 841, 907, 941
HspAI GCGC 1 cut(s) 383
Ksp22I TGATCA 2 cut(s) 355, 429
Kzo9I GATC 2 cut(s) 355, 429
Lsp1109I GCAGC 6 cut(s) 244, 247, 266, 274, 325, 688
LweI GCATC 4 cut(s) 316, 542, 801, 946
MaeII ACGT 2 cut(s) 558, 874
MaeIII GTNAC 1 cut(s) 965
MalI GATC 2 cut(s) 357, 431
MboI GATC 2 cut(s) 355, 429
MboII GAAGA 3 cut(s) 462, 644, 943
MlsI TGGCCA 1 cut(s) 83
MluCI AATT 4 cut(s) 41, 278, 912, 995
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 3 cut(s) 237, 424, 740
MnlI CCTC 7 cut(s) 104, 180, 337, 453, 504, 585, 604
Mox20I TGGCCA 1 cut(s) 83
Mph1103I ATGCAT 1 cut(s) 794
MscI TGGCCA 1 cut(s) 83
MseI TTAA 4 cut(s) 35, 681, 915, 927
MslI CAYNNNNRTG 2 cut(s) 321, 694
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 257
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 2 cut(s) 621, 981
Mva1269I GAATGC 1 cut(s) 116
MvnI CGCG 4 cut(s) 178, 342, 383, 575
MwoI GCNNNNNNNGC 6 cut(s) 187, 260, 319, 530, 551, 682
NdeII GATC 2 cut(s) 355, 429
NlaIII CATG 8 cut(s) 88, 128, 185, 554, 722, 841, 907, 941
NlaIV GGNNCC 2 cut(s) 619, 625
NmuCI GTSAC 1 cut(s) 965
NruI TCGCGA 1 cut(s) 575
NsiI ATGCAT 1 cut(s) 794
OliI CACNNNNGTG 1 cut(s) 321
PaeR7I CTCGAG 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PctI GAATGC 1 cut(s) 116
PfeI GAWTC 2 cut(s) 121, 1031
PkrI GCNGC 7 cut(s) 180, 256, 259, 262, 289, 315, 678
PleI GAGTC 3 cut(s) 237, 423, 739
PpsI GAGTC 3 cut(s) 237, 423, 739
PspFI CCCAGC 1 cut(s) 263
PspN4I GGNNCC 2 cut(s) 619, 625
PspPI GGNCC 3 cut(s) 337, 346, 623
PspXI VCTCGAGB 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 260
PvuII CAGCTG 1 cut(s) 257
RruI TCGCGA 1 cut(s) 575
RsaI GTAC 1 cut(s) 786
RsaNI GTAC 1 cut(s) 785
RseI CAYNNNNRTG 2 cut(s) 321, 694
SalI GTCGAC 1 cut(s) 88
SaqAI TTAA 4 cut(s) 35, 681, 915, 927
SatI GCNGC 7 cut(s) 179, 255, 258, 261, 288, 314, 677
Sau3AI GATC 2 cut(s) 355, 429
Sau96I GGNCC 3 cut(s) 337, 346, 623
SchI GAGTC 3 cut(s) 237, 424, 740
SfaNI GCATC 4 cut(s) 316, 542, 801, 946
SfcI CTRYAG 2 cut(s) 445, 988
Sfr274I CTCGAG 1 cut(s) 156
SinI GGWCC 2 cut(s) 337, 623
SlaI CTCGAG 1 cut(s) 156
SmiMI CAYNNNNRTG 2 cut(s) 321, 694
SmlI CTYRAG 2 cut(s) 156, 680
SmoI CTYRAG 2 cut(s) 156, 680
Sse9I AATT 4 cut(s) 41, 278, 912, 995
SsiI CCGC 4 cut(s) 178, 340, 554, 674
TaaI ACNGT 1 cut(s) 1015
TaiI ACGT 2 cut(s) 561, 877
TaqI TCGA 7 cut(s) 89, 157, 172, 406, 413, 489, 1019
TasI AATT 4 cut(s) 41, 278, 912, 995
TatI WGTACW 1 cut(s) 784
TauI GCSGC 1 cut(s) 181
TfiI GAWTC 2 cut(s) 121, 1031
Tru1I TTAA 4 cut(s) 35, 681, 915, 927
Tru9I TTAA 4 cut(s) 35, 681, 915, 927
TscAI CASTG 2 cut(s) 19, 873
TseFI GTSAC 1 cut(s) 965
TseI GCWGC 6 cut(s) 254, 257, 260, 287, 313, 676
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 5 cut(s) 17, 113, 788, 854, 924
TspGWI ACGGA 3 cut(s) 679, 729, 768
TspRI CASTG 2 cut(s) 19, 873
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 2 cut(s) 337, 623
XapI RAATTY 1 cut(s) 995
XhoI CTCGAG 1 cut(s) 156
XmiI GTMKAC 3 cut(s) 89, 725, 900
Zsp2I ATGCAT 1 cut(s) 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.