Rw3G028870

Annexin A13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017739
Physical Location & Seq
Forward (+)
43770711 .. 43772424
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G028870.1

Sequence Viewer

Length: 948 bp
ATGCCGACCCTGGAATTTGAAATCGAATGCAAAGAGATACATGATTCCTGGATACGACCACCTAAGCAGCTTAATCAATTGGTTCGATCTTTGGCACGACGAAAACGGCAAGAAAGGCAACAGATTCGAGAAACTTACAAGGCAATCTATGGAGAGGACTTGGTAAGCAGACTGCAGCACAAGGCAGATGGGGATGATGCTGATCAAATAACCACTGGGATTTCACCAAATTTGTGTGCAGCTTTGTCACTTTGGATGATTAATCAGCATGAACGTGATGCAATTTTCATCCAGGAGGCCCTTCACAATGACCAGCAAGGTCGTCATGGTAAAAACTATAATGCTCTTGTAGAGATTTTTGCTAGTCGAAAATCGAGTCAAATCCTTCTCATCAAACAAGCATATCACACAAAGTTTAGAAGGCAGTTGGACCAAGATATCATCAATATTGAGCTTCCCCATCCTCACCAAAAGATATCTGATGCACGGAGGCTTTTTGAAACAGGCCGGGAACGAAGCTCTTCAGGCATACAAGAAGCTGTCGTATTAGAATTATTTAGCAAACGCAGTATTCCACAGCTTAAGCTTACTTTTTCTTGCTATAAACACATCTATGGACATGACTATAAAAAGTACCTGAAGTCTGTAAGCTCTGGTGAATTTGAAAATGCATTGAAAATGGTCGTACAATGCATTCACAACCCACCTAATTATTTTGCAAAGATATTGCATGCAAGCATTAAAGGGTTGACAAGTAGTAGCACTAGTGATGATAAAGGGGCTTTGGTTCGTGTGATGGTGAGCAGAGCAGAAGTAGACATGGATGAGATTCAAAGAATGTTTAAGAAGAAGCATGGAGTTGAACTCAGAGACGCAATAATATCATCTGTAACGAGGGACAACTGCTACACAGACTTTCTTGTTGCCTTGGCTTCAAAATCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0010035 GO:0042221 GO:0050896 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Annexin

Protein Analysis

315

Amino Acids

36.31

Weight (kDa)

9.21

Isoelectric Point (pI)

52.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Annexin PF00191 25 - 65 1.8e-06 Annexin
Annexin PF00191 107 - 147 3.8e-07 Annexin
Annexin PF00191 175 - 226 1.2e-07 Annexin
Annexin PF00191 238 - 297 2.5e-07 Annexin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017739)

Species Orthologous Gene IDs
malus_domestica MD12G1007300.v1.1
prunus_persica Prupe.7G008200_v2.0.a1
pyrus_communis pycom12g00530
rosa_chinensis RchiOBHm_Chr3g0496581
rosa_laevigata RLG00000022517
rosa_roxburghii Rroxscaffold_6G00388900
rosa_rugosa Rorug03G0284700
rosa_samantha Rh3AG329900 Rh3BG365700 Rh3CG362700 Rh3DG365900
rosa_wichuraiana Rw3G028870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 816
AcsI RAATTY 3 cut(s) 14, 229, 659
AcuI CTGAAG 2 cut(s) 507, 659
AfaI GTAC 2 cut(s) 635, 687
AflII CTTAAG 1 cut(s) 581
AgsI TTSAA 7 cut(s) 20, 500, 665, 676, 833, 863, 936
AhlI ACTAGT 1 cut(s) 764
AjnI CCWGG 3 cut(s) 9, 47, 291
AluBI AGCT 8 cut(s) 70, 242, 454, 519, 539, 580, 586, 651
AluI AGCT 8 cut(s) 70, 242, 454, 519, 539, 580, 586, 651
Alw26I GTCTC 1 cut(s) 864
AoxI GGCC 2 cut(s) 297, 505
ApeKI GCWGC 3 cut(s) 67, 175, 239
ApoI RAATTY 3 cut(s) 14, 229, 659
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
AseI ATTAAT 1 cut(s) 261
Asp700I GAANNNNTTC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 298, 430
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 4 cut(s) 216, 458, 668, 811
AvaII GGWCC 1 cut(s) 430
BbvI GCAGC 3 cut(s) 79, 187, 251
BccI CCATC 3 cut(s) 182, 468, 790
BceAI ACGGC 1 cut(s) 122
BcgI CGANNNNNNTGC 2 cut(s) 107, 141
BciT130I CCWGG 3 cut(s) 11, 49, 293
BciVI GTATCC 1 cut(s) 45
BclI TGATCA 1 cut(s) 202
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 1 cut(s) 864
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 2 cut(s) 363, 765
BfmI CTRYAG 1 cut(s) 173
BfrI CTTAAG 1 cut(s) 581
BfuI GTATCC 1 cut(s) 45
BisI GCNGC 3 cut(s) 68, 176, 240
BlsI GCNGC 3 cut(s) 69, 177, 241
Bme1390I CCNGG 4 cut(s) 11, 49, 293, 509
Bme18I GGWCC 1 cut(s) 430
BmgT120I GGNCC 2 cut(s) 298, 430
BmrFI CCNGG 4 cut(s) 11, 49, 293, 509
BmrI ACTGGG 1 cut(s) 225
BmsI GCATC 3 cut(s) 187, 268, 472
BmuI ACTGGG 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 849, 881
Bpu10I CCTNAGC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 9, 927
Bse1I ACTGG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 3 cut(s) 11, 49, 293
BseDI CCNNGG 2 cut(s) 9, 927
BseGI GGATG 5 cut(s) 199, 261, 288, 460, 829
BseJI GATNNNNATC 1 cut(s) 201
BseMII CTCAG 1 cut(s) 880
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 3 cut(s) 79, 187, 251
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 2 cut(s) 299, 507
BsiSI CCGG 1 cut(s) 508
BslFI GGGAC 1 cut(s) 911
BsmAI GTCTC 1 cut(s) 864
BsmBI CGTCTC 1 cut(s) 864
BsmFI GGGAC 1 cut(s) 911
BsmI GAATGC 2 cut(s) 32, 693
BsnI GGCC 2 cut(s) 299, 507
Bsp143I GATC 2 cut(s) 86, 202
BspANI GGCC 2 cut(s) 299, 507
BspCNI CTCAG 1 cut(s) 879
BspMAI CTGCAG 1 cut(s) 177
BspQI GCTCTTC 1 cut(s) 526
BspTI CTTAAG 1 cut(s) 581
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 2 cut(s) 9, 927
BssMI GATC 2 cut(s) 86, 202
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 3 cut(s) 11, 49, 293
Bst6I CTCTTC 1 cut(s) 526
BstAFI CTTAAG 1 cut(s) 581
BstC8I GCNNGC 2 cut(s) 732, 736
BstDEI CTNAG 2 cut(s) 63, 866
BstF5I GGATG 5 cut(s) 199, 261, 288, 460, 829
BstKTI GATC 2 cut(s) 89, 205
BstMAI GTCTC 1 cut(s) 864
BstMBI GATC 2 cut(s) 86, 202
BstMWI GCNNNNNNNGC 2 cut(s) 115, 525
BstNI CCWGG 3 cut(s) 11, 49, 293
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 4 cut(s) 9, 47, 291, 507
BstSFI CTRYAG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 79, 187, 251
BsuI GTATCC 1 cut(s) 45
BsuRI GGCC 2 cut(s) 299, 507
BtsCI GGATG 5 cut(s) 199, 261, 288, 460, 829
BtsIMutI CAGTG 1 cut(s) 213
Cac8I GCNNGC 2 cut(s) 732, 736
Cfr13I GGNCC 2 cut(s) 298, 430
CseI GACGC 1 cut(s) 881
Csp6I GTAC 2 cut(s) 634, 686
CviAII CATG 7 cut(s) 41, 269, 326, 620, 731, 820, 854
CviQI GTAC 2 cut(s) 634, 686
DdeI CTNAG 2 cut(s) 63, 866
DpnI GATC 2 cut(s) 88, 204
DpnII GATC 2 cut(s) 86, 202
Eam1104I CTCTTC 1 cut(s) 526
EarI CTCTTC 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 927
Eco32I GATATC 2 cut(s) 439, 477
Eco47I GGWCC 1 cut(s) 430
Eco57I CTGAAG 2 cut(s) 507, 659
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 3 cut(s) 9, 47, 291
EcoRV GATATC 2 cut(s) 439, 477
EcoT14I CCWWGG 1 cut(s) 927
EcoT22I ATGCAT 2 cut(s) 673, 695
ErhI CCWWGG 1 cut(s) 927
Esp3I CGTCTC 1 cut(s) 864
FaeI CATG 7 cut(s) 44, 272, 329, 623, 734, 823, 857
FaqI GGGAC 1 cut(s) 911
FatI CATG 7 cut(s) 40, 268, 325, 619, 730, 819, 853
FbaI TGATCA 1 cut(s) 202
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 3 cut(s) 68, 176, 240
FokI GGATG 5 cut(s) 206, 268, 275, 447, 836
Fsp4HI GCNGC 3 cut(s) 68, 176, 240
FspBI CTAG 2 cut(s) 363, 765
GluI GCNGC 3 cut(s) 68, 176, 240
HaeIII GGCC 2 cut(s) 299, 507
HapII CCGG 1 cut(s) 508
HgaI GACGC 1 cut(s) 881
Hin1II CATG 7 cut(s) 44, 272, 329, 623, 734, 823, 857
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HindIII AAGCTT 1 cut(s) 584
HinfI GANTC 4 cut(s) 44, 124, 376, 829
HpaII CCGG 1 cut(s) 508
HphI GGTGA 4 cut(s) 216, 458, 668, 811
Hpy166II GTNNAC 2 cut(s) 750, 817
Hpy188I TCNGA 2 cut(s) 481, 869
Hpy188III TCNNGA 1 cut(s) 128
Hpy8I GTNNAC 2 cut(s) 750, 817
Hpy99I CGWCG 1 cut(s) 102
HpyAV CCTTC 3 cut(s) 311, 395, 414
HpyCH4IV ACGT 1 cut(s) 274
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 525
HpyF3I CTNAG 2 cut(s) 63, 866
HpySE526I ACGT 1 cut(s) 274
Hsp92II CATG 7 cut(s) 44, 272, 329, 623, 734, 823, 857
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 86, 202
LguI GCTCTTC 1 cut(s) 526
Lsp1109I GCAGC 3 cut(s) 79, 187, 251
LweI GCATC 3 cut(s) 187, 268, 472
MaeI CTAG 2 cut(s) 363, 765
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 2 cut(s) 246, 889
MalI GATC 2 cut(s) 88, 204
MboI GATC 2 cut(s) 86, 202
MboII GAAGA 2 cut(s) 513, 859
MfeI CAATTG 1 cut(s) 77
MluCI AATT 7 cut(s) 14, 77, 229, 282, 551, 659, 709
MlyI GAGTC 1 cut(s) 385
MmeI TCCRAC 1 cut(s) 408
MnlI CCTC 5 cut(s) 148, 289, 474, 483, 888
Mph1103I ATGCAT 2 cut(s) 673, 695
MroXI GAANNNNTTC 1 cut(s) 520
MseI TTAA 5 cut(s) 72, 261, 582, 741, 843
MslI CAYNNNNRTG 2 cut(s) 273, 612
MspCI CTTAAG 1 cut(s) 581
MspI CCGG 1 cut(s) 508
MspR9I CCNGG 4 cut(s) 11, 49, 293, 509
MunI CAATTG 1 cut(s) 77
Mva1269I GAATGC 2 cut(s) 32, 693
MvaI CCWGG 3 cut(s) 11, 49, 293
MwoI GCNNNNNNNGC 2 cut(s) 115, 525
NciI CCSGG 1 cut(s) 509
NdeII GATC 2 cut(s) 86, 202
NlaIII CATG 7 cut(s) 44, 272, 329, 623, 734, 823, 857
NmuCI GTSAC 1 cut(s) 246
NsiI ATGCAT 2 cut(s) 673, 695
NspI RCATGY 1 cut(s) 734
PaeI GCATGC 1 cut(s) 734
PciSI GCTCTTC 1 cut(s) 526
PctI GAATGC 2 cut(s) 32, 693
PdmI GAANNNNTTC 1 cut(s) 520
PfeI GAWTC 3 cut(s) 44, 124, 829
PfoI TCCNGGA 2 cut(s) 47, 291
PkrI GCNGC 3 cut(s) 69, 177, 241
PleI GAGTC 1 cut(s) 384
PpsI GAGTC 1 cut(s) 384
PshBI ATTAAT 1 cut(s) 261
Psp6I CCWGG 3 cut(s) 9, 47, 291
PspGI CCWGG 3 cut(s) 9, 47, 291
PspPI GGNCC 2 cut(s) 298, 430
PstI CTGCAG 1 cut(s) 177
RsaI GTAC 2 cut(s) 635, 687
RsaNI GTAC 2 cut(s) 634, 686
RseI CAYNNNNRTG 2 cut(s) 273, 612
SapI GCTCTTC 1 cut(s) 526
SaqAI TTAA 5 cut(s) 72, 261, 582, 741, 843
SatI GCNGC 3 cut(s) 68, 176, 240
Sau3AI GATC 2 cut(s) 86, 202
Sau96I GGNCC 2 cut(s) 298, 430
SchI GAGTC 1 cut(s) 385
ScrFI CCNGG 4 cut(s) 11, 49, 293, 509
SfaNI GCATC 3 cut(s) 187, 268, 472
SfcI CTRYAG 1 cut(s) 173
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 2 cut(s) 273, 612
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
SpeI ACTAGT 1 cut(s) 764
SphI GCATGC 1 cut(s) 734
Sse9I AATT 7 cut(s) 14, 77, 229, 282, 551, 659, 709
SspI AATATT 1 cut(s) 448
SspMI CTAG 2 cut(s) 363, 765
StyD4I CCNGG 4 cut(s) 9, 47, 291, 507
StyI CCWWGG 1 cut(s) 927
TaiI ACGT 1 cut(s) 277
TaqI TCGA 5 cut(s) 24, 85, 127, 367, 374
TasI AATT 7 cut(s) 14, 77, 229, 282, 551, 659, 709
TfiI GAWTC 3 cut(s) 44, 124, 829
Tru1I TTAA 5 cut(s) 72, 261, 582, 741, 843
Tru9I TTAA 5 cut(s) 72, 261, 582, 741, 843
TscAI CASTG 1 cut(s) 220
TseFI GTSAC 1 cut(s) 246
TseI GCWGC 3 cut(s) 67, 175, 239
Tsp45I GTSAC 1 cut(s) 246
TspDTI ATGAA 2 cut(s) 277, 285
TspGWI ACGGA 1 cut(s) 502
TspRI CASTG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 581
VpaK11BI GGWCC 1 cut(s) 430
VspI ATTAAT 1 cut(s) 261
XapI RAATTY 3 cut(s) 14, 229, 659
XceI RCATGY 1 cut(s) 734
XmiI GTMKAC 1 cut(s) 816
XmnI GAANNNNTTC 1 cut(s) 520
XspI CTAG 2 cut(s) 363, 765
Zsp2I ATGCAT 2 cut(s) 673, 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.