MD12G1015100.v1.1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010748
Physical Location & Seq
Reverse (-)
1549659 .. 1551812
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1015100.v1.1.491

Sequence Viewer

Length: 927 bp
ATGATGGGTCCTATTGCGGATAGGCTGCCAGCCATTTGTTCAGAAGATTCAATATCAAGGTACCTAAGAGCGCGGAACTGGAATACGAAGAAGGCAGGCAAGATGCTCAAAGAAACTATAAAATGGAGGTCGGAGTTCAAGCCCGAGAAGGTCCAATGGGAAGACATTGCTCAAGAAGCGGAGACAGGAAAAATGTACAGAGCCAATTACTATGACAAGTTTGGAAGGACAGTTCTTATCCTGAGGCCTGGTTTTCAGAACACGAATGCAGTAAACAAGCAGATTATGTATTTGGTATACTGCATGGAGAATGCCATTCTGAATATGAGTTCAGATCAGGAACAGATGGCGTGGCTAATCGATTTTCAACAGTGGAACACATCAAGCATATCGTTGAAGGCGACTAGGGAAACAGCAAAGATCCTGCAAAATCATTACCCTGAGAGATTGGGAATTGGCATCCTCTATAATCCACCAAAAGTATTTGAGTCCTTTTGGACGATGGTAAAACCATTTCTTGAGCCGAAAACACACAAGAAGGTGAAGTTTGTGTACTCGGACAACCCGCAGAGCAAGAAGGTAATGGAAGATCTTTTCAACATGGACAAGTTGGAATCTGCATTTGGTGGAAGAAGTTCAGGTGGTTTTGACTATGAAGCTTACGGACAAAGAATGCGAGAAGATGACAAGAAAATGTCTAGTTTCGTTGACTCTGGCTGTTCGTCAGCTTACTCACCATCAATCATGTCTGAGTTGCAGCGGTCGGAGTTGTCTTCTGACCAATGTTCCGAGGCCTCTGATGAAGGCGGTTGCTCGTCGTGCGACAAAGCAGCTTCGTCGAACGTGGAGGTTGCCAATGAGAAGGTACAAGGACAACCGCACGACAGCAAAGATGTTGCAAATGGCAACACAAAAGTAGGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

309

Amino Acids

34.97

Weight (kDa)

6.85

Isoelectric Point (pI)

53.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 62 - 210 1e-32 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 60
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 73
AciI CCGC 7 cut(s) 17, 73, 179, 566, 760, 807, 878
AclWI GGATC 1 cut(s) 415
AfaI GTAC 4 cut(s) 62, 197, 554, 867
AgsI TTSAA 5 cut(s) 51, 139, 368, 397, 598
AjnI CCWGG 1 cut(s) 247
AjuI GAANNNNNNNTTGG 2 cut(s) 606, 638
AloI GAACNNNNNNTCC 2 cut(s) 216, 248
AluBI AGCT 3 cut(s) 659, 728, 833
AluI AGCT 3 cut(s) 659, 728, 833
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 143
AoxI GGCC 2 cut(s) 245, 792
ApeKI GCWGC 3 cut(s) 25, 757, 830
Asp700I GAANNNNTTC 1 cut(s) 634
Asp718I GGTACC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 73
AspS9I GGNCC 2 cut(s) 8, 151
AsuHPI GGTGA 2 cut(s) 553, 726
AvaI CYCGRG 1 cut(s) 143
AvaII GGWCC 2 cut(s) 8, 151
AxyI CCTNAGG 1 cut(s) 242
BanI GGYRCC 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 536, 568
BbsI GAAGAC 2 cut(s) 168, 765
BbvI GCAGC 3 cut(s) 12, 769, 842
BccI CCATC 3 cut(s) 340, 496, 745
BcgI CGANNNNNNTGC 2 cut(s) 819, 853
BciT130I CCWGG 1 cut(s) 249
BcoDI GTCTC 1 cut(s) 176
BfaI CTAG 2 cut(s) 405, 699
BglII AGATCT 1 cut(s) 589
BisI GCNGC 3 cut(s) 26, 758, 831
BlsI GCNGC 3 cut(s) 27, 759, 832
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 2 cut(s) 8, 151
BmeT110I CYCGRG 1 cut(s) 143
BmgT120I GGNCC 2 cut(s) 8, 151
BmiI GGNNCC 2 cut(s) 9, 62
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 2 cut(s) 93, 468
BpiI GAAGAC 2 cut(s) 168, 765
BpuEI CTTGAG 2 cut(s) 156, 539
Bsa29I ATCGAT 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 789
Bse1I ACTGG 1 cut(s) 83
Bse21I CCTNAGG 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 165
BseBI CCWGG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 789
BseGI GGATG 1 cut(s) 459
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 3 cut(s) 233, 432, 741
BseNI ACTGG 1 cut(s) 83
BseXI GCAGC 3 cut(s) 12, 769, 842
Bsh1236I CGCG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 2 cut(s) 247, 794
BshNI GGYRCC 1 cut(s) 60
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 764
BsiHKCI CYCGRG 1 cut(s) 143
BsmAI GTCTC 1 cut(s) 176
BsmI GAATGC 3 cut(s) 271, 316, 678
BsnI GGCC 2 cut(s) 247, 794
BsoBI CYCGRG 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 3 cut(s) 334, 420, 589
BspACI CCGC 7 cut(s) 17, 73, 179, 566, 760, 807, 878
BspANI GGCC 2 cut(s) 247, 794
BspCNI CTCAG 3 cut(s) 234, 433, 742
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 9, 62
BspPI GGATC 1 cut(s) 415
BspT107I GGYRCC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 165
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 789
BssMI GATC 3 cut(s) 334, 420, 589
BssNAI GTATAC 1 cut(s) 298
Bst1107I GTATAC 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 232, 372
BstAUI TGTACA 1 cut(s) 195
BstC8I GCNNGC 2 cut(s) 30, 97
BstDEI CTNAG 4 cut(s) 65, 242, 441, 750
BstF5I GGATG 1 cut(s) 459
BstFNI CGCG 1 cut(s) 73
BstHHI GCGC 1 cut(s) 73
BstKTI GATC 3 cut(s) 337, 423, 592
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 3 cut(s) 334, 420, 589
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 2 cut(s) 176, 819
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 3 cut(s) 12, 769, 842
BstV2I GAAGAC 2 cut(s) 168, 765
BstX2I RGATCY 2 cut(s) 420, 589
BstYI RGATCY 2 cut(s) 420, 589
BstZ17I GTATAC 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 360
Bsu36I CCTNAGG 1 cut(s) 242
BsuRI GGCC 2 cut(s) 247, 794
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 377
Cac8I GCNNGC 2 cut(s) 30, 97
CfoI GCGC 1 cut(s) 73
Cfr13I GGNCC 2 cut(s) 8, 151
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 4 cut(s) 61, 196, 553, 866
CviAII CATG 3 cut(s) 304, 601, 745
CviQI GTAC 4 cut(s) 61, 196, 553, 866
DdeI CTNAG 4 cut(s) 65, 242, 441, 750
DpnI GATC 3 cut(s) 336, 422, 591
DpnII GATC 3 cut(s) 334, 420, 589
Eco147I AGGCCT 2 cut(s) 247, 794
Eco47I GGWCC 2 cut(s) 8, 151
Eco81I CCTNAGG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 8
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 3 cut(s) 307, 604, 748
FatI CATG 3 cut(s) 303, 600, 744
FauI CCCGC 1 cut(s) 573
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 3 cut(s) 26, 758, 831
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 3 cut(s) 26, 758, 831
FspBI CTAG 2 cut(s) 405, 699
GlaI GCGC 1 cut(s) 72
GluI GCNGC 3 cut(s) 26, 758, 831
HaeIII GGCC 2 cut(s) 247, 794
HhaI GCGC 1 cut(s) 73
Hin1II CATG 3 cut(s) 307, 604, 748
Hin6I GCGC 1 cut(s) 71
HinP1I GCGC 1 cut(s) 71
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 657
HinfI GANTC 4 cut(s) 47, 488, 614, 710
HphI GGTGA 2 cut(s) 553, 726
Hpy166II GTNNAC 4 cut(s) 274, 298, 553, 709
Hpy188III TCNNGA 4 cut(s) 173, 241, 338, 518
Hpy8I GTNNAC 4 cut(s) 274, 298, 553, 709
Hpy99I CGWCG 2 cut(s) 820, 841
HpyAV CCTTC 8 cut(s) 85, 142, 219, 391, 532, 571, 797, 856
HpyCH4III ACNGT 2 cut(s) 232, 372
HpyCH4IV ACGT 1 cut(s) 843
HpyCH4V TGCA 6 cut(s) 269, 303, 427, 620, 757, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 176, 819
HpyF3I CTNAG 4 cut(s) 65, 242, 441, 750
HpySE526I ACGT 1 cut(s) 843
Hsp92II CATG 3 cut(s) 307, 604, 748
HspAI GCGC 1 cut(s) 71
KpnI GGTACC 1 cut(s) 64
Kzo9I GATC 3 cut(s) 334, 420, 589
Lsp1109I GCAGC 3 cut(s) 12, 769, 842
LweI GCATC 2 cut(s) 93, 468
MaeI CTAG 2 cut(s) 405, 699
MaeII ACGT 1 cut(s) 843
MalI GATC 3 cut(s) 336, 422, 591
MboI GATC 3 cut(s) 334, 420, 589
MboII GAAGA 7 cut(s) 56, 100, 173, 599, 642, 692, 765
MflI RGATCY 2 cut(s) 420, 589
MluCI AATT 2 cut(s) 205, 453
MlyI GAGTC 2 cut(s) 497, 704
MmeI TCCRAC 3 cut(s) 111, 591, 744
MnlI CCTC 6 cut(s) 120, 237, 473, 784, 805, 841
MroXI GAANNNNTTC 1 cut(s) 634
MspA1I CMGCKG 1 cut(s) 760
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 3 cut(s) 271, 316, 678
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 176, 819
NdeII GATC 3 cut(s) 334, 420, 589
NlaIII CATG 3 cut(s) 307, 604, 748
NlaIV GGNNCC 2 cut(s) 9, 62
PceI AGGCCT 2 cut(s) 247, 794
PcsI WCGNNNNNNNCGW 1 cut(s) 398
PctI GAATGC 3 cut(s) 271, 316, 678
PdmI GAANNNNTTC 1 cut(s) 634
PfeI GAWTC 2 cut(s) 47, 614
PkrI GCNGC 3 cut(s) 27, 759, 832
PleI GAGTC 2 cut(s) 496, 704
PpsI GAGTC 2 cut(s) 496, 704
PpuMI RGGWCCY 1 cut(s) 8
Psp5II RGGWCCY 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 2 cut(s) 9, 62
PspPI GGNCC 2 cut(s) 8, 151
PspPPI RGGWCCY 1 cut(s) 8
PsuI RGATCY 2 cut(s) 420, 589
RsaI GTAC 4 cut(s) 62, 197, 554, 867
RsaNI GTAC 4 cut(s) 61, 196, 553, 866
SatI GCNGC 3 cut(s) 26, 758, 831
Sau3AI GATC 3 cut(s) 334, 420, 589
Sau96I GGNCC 2 cut(s) 8, 151
SchI GAGTC 2 cut(s) 497, 704
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 2 cut(s) 93, 468
SinI GGWCC 2 cut(s) 8, 151
SmlI CTYRAG 2 cut(s) 171, 518
SmoI CTYRAG 2 cut(s) 171, 518
Sse9I AATT 2 cut(s) 205, 453
SseBI AGGCCT 2 cut(s) 247, 794
SsiI CCGC 7 cut(s) 17, 73, 179, 566, 760, 807, 878
SspMI CTAG 2 cut(s) 405, 699
StuI AGGCCT 2 cut(s) 247, 794
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 232, 372
TaiI ACGT 1 cut(s) 846
TaqI TCGA 2 cut(s) 360, 839
TasI AATT 2 cut(s) 205, 453
TatI WGTACW 2 cut(s) 195, 552
TfiI GAWTC 2 cut(s) 47, 614
TscAI CASTG 1 cut(s) 377
TseI GCWGC 3 cut(s) 25, 757, 830
TspDTI ATGAA 2 cut(s) 669, 816
TspGWI ACGGA 1 cut(s) 678
TspRI CASTG 1 cut(s) 377
VpaK11BI GGWCC 2 cut(s) 8, 151
XmiI GTMKAC 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 634
XspI CTAG 2 cut(s) 405, 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.