MD14G1013100.v1.1

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010748
Physical Location & Seq
Reverse (-)
1183665 .. 1186491
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1013100.v1.1.491

Sequence Viewer

Length: 1029 bp
ATGTCATTCAAAAAAGGAGAAAGCACAACAGATAAAGCATTGTCACATGGAGAGCAGCTGGCCAAGATTTATGAAGTAAGGAAGATGATGGGTCCTATTGCGGATAGGCTACCAGCCATTTGTTCAGAAGCTTCAATCTCAAGGTACCTAAGAGCGCGGAACTGGAATACAAAGAAGGCGGGCAAGATGCTGAAAGAAACCATAAAATGGAGGTTGGAGTTCAAGCCCGAGAAGATCCGATGGGAAAACATTACTCAAGAAGCGAAGACAGGAAAAATTTACAGAGCCAATTACCATGACAAGTGCGGAAGGACAGTTCTTATCCTGAGGCCTGGTTTTCAGAACACAAATGCGGTAAACGAGCAGATGATGTATTTGGTATACTGCCTGGAGAATGCCATACTGAATATGAATCCAGATCAGGAACAGATGGTGTGGCTAATTGATTTTCAACGGTGGAACACGTCGAGCATATCGTTGAAGGCGACTAGGGAAACAGCTAAGATCCTGCAAAATCATTACCCTGAGAGATTGGGAATTGGCATCCTCTATAATCCACCAAAAATATTTGAGTCCTTTTGGACGTTGGTAAAACCATTTCTTGAGCCAAAAACACACAAGAAGATGAAGTTTGTGTACTCGGACAACTCGCAGAGCCAGAAGATAATGGAAGATCTTTTCAACATGGACAAGTTGGAATCCACATTTGGCGGGAGAAGTTCAGTTGGTTTCGACTATGAAGCTTACGGACAAAGAATGCGAGAAGATGACAAGAAAATGTCTAGTTTTATCGATTCAGGTTGTTCGCCAGCCTATTCGCCAGCCTATTCGCCAGCTTATTCGCCATCAATCATGTCCGAGTTGCAGCAATTGACTTCTGACCATTGTTCTGAGGTCTCCGATGAAGGCGGCTGCTCGTCGTGCGATGAAGCAGCTTCGTCGAACTTGGAGGTCGTCGATGAGAAGGTACAAGGACAACCGCACGACAGCAAAGATGTTGCAAATGACTGCACAAAAGTGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

343

Amino Acids

39.04

Weight (kDa)

7.54

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 89 - 238 2.9e-34 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 381
AccII CGCG 1 cut(s) 157
AciI CCGC 8 cut(s) 101, 157, 179, 306, 353, 711, 909, 980
AclWI GGATC 2 cut(s) 229, 499
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 1 cut(s) 276
AfaI GTAC 3 cut(s) 146, 638, 969
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 462
AgsI TTSAA 6 cut(s) 10, 135, 223, 452, 481, 682
AjiI CACGTC 1 cut(s) 465
AjnI CCWGG 2 cut(s) 331, 387
AjuI GAANNNNNNNTTGG 2 cut(s) 690, 722
AleI CACNNNNGTG 1 cut(s) 1016
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 6 cut(s) 58, 131, 500, 743, 836, 935
AluI AGCT 6 cut(s) 58, 131, 500, 743, 836, 935
Alw26I GTCTC 1 cut(s) 901
AlwI GGATC 2 cut(s) 229, 499
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 2 cut(s) 60, 329
ApeKI GCWGC 5 cut(s) 55, 865, 912, 932, 1022
ApoI RAATTY 1 cut(s) 276
Asp718I GGTACC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 326
BalI TGGCCA 1 cut(s) 62
BanI GGYRCC 1 cut(s) 144
BarI GAAGNNNNNNTAC 2 cut(s) 620, 652
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 5 cut(s) 67, 877, 899, 944, 1009
BccI CCATC 4 cut(s) 82, 234, 424, 853
BcgI CGANNNNNNTGC 2 cut(s) 921, 955
BciT130I CCWGG 2 cut(s) 333, 389
BcoDI GTCTC 1 cut(s) 901
BfaI CTAG 2 cut(s) 489, 783
BglII AGATCT 1 cut(s) 673
BisI GCNGC 6 cut(s) 56, 866, 910, 913, 933, 1023
BlsI GCNGC 6 cut(s) 57, 867, 911, 914, 934, 1024
Bme1390I CCNGG 2 cut(s) 333, 389
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 93, 146
BmrFI CCNGG 2 cut(s) 333, 389
BmsI GCATC 2 cut(s) 177, 552
BpiI GAAGAC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 410
BpuEI CTTGAG 3 cut(s) 124, 240, 623
Bsa29I ATCGAT 1 cut(s) 792
BsaI GGTCTC 1 cut(s) 901
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 326
BseBI CCWGG 2 cut(s) 333, 389
BseCI ATCGAT 1 cut(s) 792
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 317, 516, 882
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 5 cut(s) 67, 877, 899, 944, 1009
BsgI GTGCAG 1 cut(s) 994
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 62, 331
BshNI GGYRCC 1 cut(s) 144
BshVI ATCGAT 1 cut(s) 792
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 901
BsmI GAATGC 2 cut(s) 400, 762
BsnI GGCC 2 cut(s) 62, 331
Bso31I GGTCTC 1 cut(s) 901
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 4 cut(s) 234, 418, 504, 673
BspACI CCGC 8 cut(s) 101, 157, 179, 306, 353, 711, 909, 980
BspANI GGCC 2 cut(s) 62, 331
BspCNI CTCAG 3 cut(s) 318, 517, 883
BspDI ATCGAT 1 cut(s) 792
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 93, 146
BspPI GGATC 2 cut(s) 229, 499
BspT107I GGYRCC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 901
BsrI ACTGG 1 cut(s) 167
BssMI GATC 4 cut(s) 234, 418, 504, 673
BssNAI GTATAC 1 cut(s) 382
Bst1107I GTATAC 1 cut(s) 382
Bst2UI CCWGG 2 cut(s) 333, 389
Bst4CI ACNGT 2 cut(s) 316, 456
BstC8I GCNNGC 5 cut(s) 60, 181, 810, 822, 834
BstDEI CTNAG 5 cut(s) 149, 326, 501, 525, 891
BstF5I GGATG 1 cut(s) 543
BstFNI CGCG 1 cut(s) 157
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 4 cut(s) 237, 421, 507, 676
BstMAI GTCTC 1 cut(s) 901
BstMBI GATC 4 cut(s) 234, 418, 504, 673
BstMWI GCNNNNNNNGC 1 cut(s) 921
BstNI CCWGG 2 cut(s) 333, 389
BstSCI CCNGG 2 cut(s) 331, 387
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 5 cut(s) 67, 877, 899, 944, 1009
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 3 cut(s) 234, 504, 673
BstYI RGATCY 3 cut(s) 234, 504, 673
BstZ17I GTATAC 1 cut(s) 382
Bsu15I ATCGAT 1 cut(s) 792
Bsu36I CCTNAGG 1 cut(s) 326
BsuRI GGCC 2 cut(s) 62, 331
BsuTUI ATCGAT 1 cut(s) 792
BtgZI GCGATG 1 cut(s) 939
BtrI CACGTC 1 cut(s) 465
BtsCI GGATG 1 cut(s) 543
Cac8I GCNNGC 5 cut(s) 60, 181, 810, 822, 834
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 92
ClaI ATCGAT 1 cut(s) 792
Csp6I GTAC 3 cut(s) 145, 637, 968
CviAII CATG 5 cut(s) 47, 296, 685, 853, 1026
CviQI GTAC 3 cut(s) 145, 637, 968
DdeI CTNAG 5 cut(s) 149, 326, 501, 525, 891
DpnI GATC 4 cut(s) 236, 420, 506, 675
DpnII GATC 4 cut(s) 234, 418, 504, 673
EaeI YGGCCR 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 331
Eco31I GGTCTC 1 cut(s) 901
Eco47I GGWCC 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 2 cut(s) 331, 387
FaeI CATG 5 cut(s) 50, 299, 688, 856, 1029
FatI CATG 5 cut(s) 46, 295, 684, 852, 1025
FauI CCCGC 2 cut(s) 172, 704
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 6 cut(s) 56, 866, 910, 913, 933, 1023
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 6 cut(s) 56, 866, 910, 913, 933, 1023
FspBI CTAG 2 cut(s) 489, 783
GlaI GCGC 1 cut(s) 156
GluI GCNGC 6 cut(s) 56, 866, 910, 913, 933, 1023
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 2 cut(s) 62, 331
HhaI GCGC 1 cut(s) 157
Hin1II CATG 5 cut(s) 50, 299, 688, 856, 1029
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HindIII AAGCTT 2 cut(s) 129, 741
HinfI GANTC 4 cut(s) 412, 572, 698, 794
Hpy166II GTNNAC 3 cut(s) 358, 382, 637
Hpy188I TCNGA 8 cut(s) 127, 239, 342, 643, 859, 880, 892, 901
Hpy188III TCNNGA 5 cut(s) 257, 325, 416, 422, 602
Hpy8I GTNNAC 3 cut(s) 358, 382, 637
Hpy99I CGWCG 4 cut(s) 469, 922, 943, 959
HpyAV CCTTC 5 cut(s) 169, 303, 475, 899, 958
HpyCH4III ACNGT 2 cut(s) 316, 456
HpyCH4IV ACGT 2 cut(s) 464, 584
HpyCH4V TGCA 5 cut(s) 511, 865, 1001, 1011, 1025
HpyF10VI GCNNNNNNNGC 1 cut(s) 921
HpyF3I CTNAG 5 cut(s) 149, 326, 501, 525, 891
HpySE526I ACGT 2 cut(s) 464, 584
Hsp92II CATG 5 cut(s) 50, 299, 688, 856, 1029
HspAI GCGC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 148
Kzo9I GATC 4 cut(s) 234, 418, 504, 673
Lsp1109I GCAGC 5 cut(s) 67, 877, 899, 944, 1009
LweI GCATC 2 cut(s) 177, 552
MaeI CTAG 2 cut(s) 489, 783
MaeII ACGT 2 cut(s) 464, 584
MaeIII GTNAC 1 cut(s) 42
MalI GATC 4 cut(s) 236, 420, 506, 675
MboI GATC 4 cut(s) 234, 418, 504, 673
MboII GAAGA 7 cut(s) 94, 244, 277, 634, 673, 683, 776
MfeI CAATTG 1 cut(s) 869
MflI RGATCY 3 cut(s) 234, 504, 673
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 5 cut(s) 276, 289, 441, 537, 869
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 2 cut(s) 195, 675
MnlI CCTC 5 cut(s) 204, 321, 557, 886, 943
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MslI CAYNNNNRTG 1 cut(s) 1016
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 58
MspR9I CCNGG 2 cut(s) 333, 389
MunI CAATTG 1 cut(s) 869
Mva1269I GAATGC 2 cut(s) 400, 762
MvaI CCWGG 2 cut(s) 333, 389
MvnI CGCG 1 cut(s) 157
MwoI GCNNNNNNNGC 1 cut(s) 921
NdeII GATC 4 cut(s) 234, 418, 504, 673
NlaIII CATG 5 cut(s) 50, 299, 688, 856, 1029
NlaIV GGNNCC 2 cut(s) 93, 146
NmuCI GTSAC 1 cut(s) 42
OliI CACNNNNGTG 1 cut(s) 1016
PceI AGGCCT 1 cut(s) 331
PcsI WCGNNNNNNNCGW 2 cut(s) 473, 482
PctI GAATGC 2 cut(s) 400, 762
PfeI GAWTC 3 cut(s) 412, 698, 794
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 6 cut(s) 57, 867, 911, 914, 934, 1024
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 2 cut(s) 331, 387
PspGI CCWGG 2 cut(s) 331, 387
PspN4I GGNNCC 2 cut(s) 93, 146
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PsuI RGATCY 3 cut(s) 234, 504, 673
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 3 cut(s) 146, 638, 969
RsaNI GTAC 3 cut(s) 145, 637, 968
RseI CAYNNNNRTG 1 cut(s) 1016
SatI GCNGC 6 cut(s) 56, 866, 910, 913, 933, 1023
Sau3AI GATC 4 cut(s) 234, 418, 504, 673
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 2 cut(s) 333, 389
SfaNI GCATC 2 cut(s) 177, 552
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 1016
SmlI CTYRAG 3 cut(s) 139, 255, 602
SmoI CTYRAG 3 cut(s) 139, 255, 602
Sse9I AATT 5 cut(s) 276, 289, 441, 537, 869
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 8 cut(s) 101, 157, 179, 306, 353, 711, 909, 980
SspI AATATT 1 cut(s) 567
SspMI CTAG 2 cut(s) 489, 783
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 2 cut(s) 331, 387
TaaI ACNGT 2 cut(s) 316, 456
TaiI ACGT 2 cut(s) 467, 587
TaqI TCGA 5 cut(s) 467, 732, 792, 941, 957
TasI AATT 5 cut(s) 276, 289, 441, 537, 869
TatI WGTACW 1 cut(s) 636
TauI GCSGC 1 cut(s) 912
TfiI GAWTC 3 cut(s) 412, 698, 794
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 5 cut(s) 55, 865, 912, 932, 1022
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 6 cut(s) 87, 425, 641, 753, 918, 942
TspGWI ACGGA 1 cut(s) 762
Van91I CCANNNNNTGG 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 381
XspI CTAG 2 cut(s) 489, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.