Rorug03G0273800

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0002241
Physical Location & Seq
Reverse (-)
27066339 .. 27068198
1860 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0273800.1

Sequence Viewer

Length: 609 bp
ATGGCAGCTGGGAACCCAGTGTATGATAAATCCGTACTTAGAGGAGTTCAAGAGGCTACTGGTTCAAATCATGATCTCAACTATCAGTTTGTGGACCAGGCAACGGATTGCATAGACTGTGCAGCAGATGCGTCGGTGGTGAGTGCGGTAAATGAGGGGATTGATGCAGCATTGGAGATTTTCCCTCTTCCAAGGCCTTGGGTGATGATCAGTGTGAATGATGATGAAGATCTTCACTTTCGAAAAGCTGAATTTGATCCAGAGGATTGTCCACTTGACTGTTCAAGGCCTTGTGAGAGAGTATGTCATGCTGATGCAATATCCTTTGAGGCTGGAAGATCAATTACACAATTCTCTTATGCCACCAAAACACCTGCCTCTTTAAAGGCCGGAGTTCTAACTGAGCGCTGCTATGGCTGTGGCCGCTGCATTCCAGTCTGCCCATACGATAAAATAAGTGTGAGCACATACATAAGAGATGCTACTGCAACATCTGAACTTCTCAAAAGAAATGATGTTGATGCTATAGAGATTCATACAAGTGGAAGATTGCTCCATTTAAAGAACTTTGGGATGGTTTGGGAGATTCAATCAAACATCTCAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

202

Amino Acids

22.21

Weight (kDa)

4.7

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LdpA_Fe-S-bd PF25160 90 - 157 9e-20 LdpA Fe-S cluster-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 382
Acc36I ACCTGC 1 cut(s) 382
AciI CCGC 2 cut(s) 146, 424
AclWI GGATC 1 cut(s) 251
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 1 cut(s) 36
AfeI AGCGCT 1 cut(s) 407
AfiI CCNNNNNNNGG 1 cut(s) 103
AgsI TTSAA 4 cut(s) 50, 66, 285, 590
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 2 cut(s) 8, 248
AluI AGCT 2 cut(s) 8, 248
Alw21I GWGCWC 1 cut(s) 467
AlwI GGATC 1 cut(s) 251
Aor51HI AGCGCT 1 cut(s) 407
AoxI GGCC 4 cut(s) 194, 287, 387, 421
ApeKI GCWGC 5 cut(s) 5, 122, 167, 408, 426
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 231
AspLEI GCGC 1 cut(s) 408
AspS9I GGNCC 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 151, 214
AsuII TTCGAA 1 cut(s) 241
AvaII GGWCC 1 cut(s) 94
BarI GAAGNNNNNNTAC 2 cut(s) 328, 360
Bbv12I GWGCWC 1 cut(s) 467
BbvI GCAGC 5 cut(s) 17, 134, 179, 395, 413
BccI CCATC 1 cut(s) 568
BcgI CGANNNNNNTGC 2 cut(s) 114, 148
BciT130I CCWGG 1 cut(s) 98
BclI TGATCA 1 cut(s) 207
BfaI CTAG 1 cut(s) 607
BfmI CTRYAG 1 cut(s) 525
BfoI RGCGCY 1 cut(s) 409
BfuAI ACCTGC 1 cut(s) 382
BglII AGATCT 1 cut(s) 229
BisI GCNGC 6 cut(s) 6, 123, 168, 409, 424, 427
BlsI GCNGC 6 cut(s) 7, 124, 169, 410, 425, 428
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 1 cut(s) 14
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 1 cut(s) 11
BmsI GCATC 5 cut(s) 118, 154, 304, 469, 511
BmuI ACTGGG 1 cut(s) 11
Bpu14I TTCGAA 1 cut(s) 241
BsaBI GATNNNNATC 1 cut(s) 228
BsaJI CCNNGG 2 cut(s) 191, 197
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 17, 64, 434
Bse8I GATNNNNATC 1 cut(s) 228
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 191, 197
BseGI GGATG 1 cut(s) 579
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 1 cut(s) 103
BseMII CTCAG 1 cut(s) 393
BseNI ACTGG 3 cut(s) 17, 64, 434
BseRI GAGGAG 1 cut(s) 57
BseXI GCAGC 5 cut(s) 17, 134, 179, 395, 413
BseYI CCCAGC 1 cut(s) 8
BsgI GTGCAG 1 cut(s) 141
BshFI GGCC 4 cut(s) 196, 289, 389, 423
BsiHKAI GWGCWC 1 cut(s) 467
BsiSI CCGG 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 103
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 4 cut(s) 196, 289, 389, 423
Bsp119I TTCGAA 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 467
Bsp143I GATC 5 cut(s) 73, 207, 229, 256, 338
BspACI CCGC 2 cut(s) 146, 424
BspANI GGCC 4 cut(s) 196, 289, 389, 423
BspCNI CTCAG 1 cut(s) 394
BspHI TCATGA 1 cut(s) 70
BspLI GGNNCC 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 382
BspPI GGATC 1 cut(s) 251
BspT104I TTCGAA 1 cut(s) 241
BsrI ACTGG 3 cut(s) 17, 64, 434
BssECI CCNNGG 2 cut(s) 191, 197
BssMI GATC 5 cut(s) 73, 207, 229, 256, 338
BssT1I CCWWGG 2 cut(s) 191, 197
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 119, 281
Bst6I CTCTTC 1 cut(s) 192
BstAPI GCANNNNNTGC 1 cut(s) 128
BstBI TTCGAA 1 cut(s) 241
BstDEI CTNAG 3 cut(s) 38, 402, 601
BstF5I GGATG 1 cut(s) 579
BstH2I RGCGCY 1 cut(s) 409
BstHHI GCGC 1 cut(s) 408
BstKTI GATC 5 cut(s) 76, 210, 232, 259, 341
BstMBI GATC 5 cut(s) 73, 207, 229, 256, 338
BstMWI GCNNNNNNNGC 3 cut(s) 128, 414, 423
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 5 cut(s) 17, 134, 179, 395, 413
BstX2I RGATCY 1 cut(s) 229
BstXI CCANNNNNNTGG 1 cut(s) 198
BstYI RGATCY 1 cut(s) 229
BsuRI GGCC 4 cut(s) 196, 289, 389, 423
BtsCI GGATG 1 cut(s) 579
BtsIMutI CAGTG 2 cut(s) 24, 217
BveI ACCTGC 1 cut(s) 382
CciI TCATGA 1 cut(s) 70
CfoI GCGC 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 94
CseI GACGC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 35
CviAII CATG 2 cut(s) 71, 308
CviJI RGCY 9 cut(s) 8, 56, 196, 248, 289, 332, 389, 417, 423
CviKI_1 RGCY 9 cut(s) 8, 56, 196, 248, 289, 332, 389, 417, 423
CviQI GTAC 1 cut(s) 35
DdeI CTNAG 3 cut(s) 38, 402, 601
DpnI GATC 5 cut(s) 75, 209, 231, 258, 340
DpnII GATC 5 cut(s) 73, 207, 229, 256, 338
DraI TTTAAA 2 cut(s) 384, 561
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 191, 197
Eco147I AGGCCT 2 cut(s) 196, 289
Eco47I GGWCC 1 cut(s) 94
Eco47III AGCGCT 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 191, 197
ErhI CCWWGG 2 cut(s) 191, 197
FaeI CATG 2 cut(s) 74, 311
FatI CATG 2 cut(s) 70, 307
FbaI TGATCA 1 cut(s) 207
Fnu4HI GCNGC 6 cut(s) 6, 123, 168, 409, 424, 427
FokI GGATG 1 cut(s) 586
Fsp4HI GCNGC 6 cut(s) 6, 123, 168, 409, 424, 427
FspBI CTAG 1 cut(s) 607
GlaI GCGC 1 cut(s) 407
GluI GCNGC 6 cut(s) 6, 123, 168, 409, 424, 427
GsaI CCCAGC 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 409
HaeIII GGCC 4 cut(s) 196, 289, 389, 423
HapII CCGG 1 cut(s) 390
HgaI GACGC 1 cut(s) 120
HhaI GCGC 1 cut(s) 408
Hin1II CATG 2 cut(s) 74, 311
Hin6I GCGC 1 cut(s) 406
HinP1I GCGC 1 cut(s) 406
HinfI GANTC 2 cut(s) 532, 586
HpaII CCGG 1 cut(s) 390
HphI GGTGA 2 cut(s) 151, 214
Hpy166II GTNNAC 2 cut(s) 94, 272
Hpy188I TCNGA 2 cut(s) 496, 604
Hpy188III TCNNGA 3 cut(s) 50, 71, 260
Hpy8I GTNNAC 2 cut(s) 94, 272
Hpy99I CGWCG 1 cut(s) 136
HpyCH4III ACNGT 2 cut(s) 119, 281
HpyCH4V TGCA 6 cut(s) 111, 122, 167, 317, 429, 488
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 414, 423
HpyF3I CTNAG 3 cut(s) 38, 402, 601
Hsp92II CATG 2 cut(s) 74, 311
HspAI GCGC 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 207
Kzo9I GATC 5 cut(s) 73, 207, 229, 256, 338
LmnI GCTCC 1 cut(s) 558
LpnPI CCDG 9 cut(s) 30, 45, 83, 110, 273, 318, 387, 403, 447
Lsp1109I GCAGC 5 cut(s) 17, 134, 179, 395, 413
LweI GCATC 5 cut(s) 118, 154, 304, 469, 511
MaeI CTAG 1 cut(s) 607
MalI GATC 5 cut(s) 75, 209, 231, 258, 340
MboI GATC 5 cut(s) 73, 207, 229, 256, 338
MboII GAAGA 5 cut(s) 179, 224, 239, 348, 558
MflI RGATCY 1 cut(s) 229
MhlI GDGCHC 1 cut(s) 467
MluCI AATT 3 cut(s) 251, 342, 350
MnlI CCTC 7 cut(s) 35, 46, 148, 195, 256, 322, 388
MroXI GAANNNNTTC 1 cut(s) 231
MseI TTAA 2 cut(s) 383, 560
MslI CAYNNNNRTG 2 cut(s) 312, 540
MspA1I CMGCKG 2 cut(s) 8, 426
MspI CCGG 1 cut(s) 390
MspR9I CCNGG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 429
MvaI CCWGG 1 cut(s) 98
MwoI GCNNNNNNNGC 3 cut(s) 128, 414, 423
NdeII GATC 5 cut(s) 73, 207, 229, 256, 338
NlaIII CATG 2 cut(s) 74, 311
NlaIV GGNNCC 1 cut(s) 14
NspV TTCGAA 1 cut(s) 241
PagI TCATGA 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 382
PceI AGGCCT 2 cut(s) 196, 289
PctI GAATGC 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 231
PfeI GAWTC 2 cut(s) 532, 586
PkrI GCNGC 6 cut(s) 7, 124, 169, 410, 425, 428
Psp6I CCWGG 1 cut(s) 96
PspFI CCCAGC 1 cut(s) 8
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 14
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 36
RsaNI GTAC 1 cut(s) 35
RseI CAYNNNNRTG 2 cut(s) 312, 540
SaqAI TTAA 2 cut(s) 383, 560
SatI GCNGC 6 cut(s) 6, 123, 168, 409, 424, 427
Sau3AI GATC 5 cut(s) 73, 207, 229, 256, 338
Sau96I GGNCC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 1 cut(s) 467
SetI ASST 3 cut(s) 10, 250, 376
SfaNI GCATC 5 cut(s) 118, 154, 304, 469, 511
SfcI CTRYAG 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 241
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 2 cut(s) 312, 540
Sse9I AATT 3 cut(s) 251, 342, 350
SseBI AGGCCT 2 cut(s) 196, 289
SsiI CCGC 2 cut(s) 146, 424
SspMI CTAG 1 cut(s) 607
StuI AGGCCT 2 cut(s) 196, 289
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 2 cut(s) 191, 197
TaaI ACNGT 2 cut(s) 119, 281
TaqI TCGA 1 cut(s) 241
TasI AATT 3 cut(s) 251, 342, 350
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 2 cut(s) 532, 586
Tru1I TTAA 2 cut(s) 383, 560
Tru9I TTAA 2 cut(s) 383, 560
TscAI CASTG 2 cut(s) 24, 217
TseI GCWGC 5 cut(s) 5, 122, 167, 408, 426
TspDTI ATGAA 2 cut(s) 240, 524
TspGWI ACGGA 2 cut(s) 22, 119
TspRI CASTG 2 cut(s) 24, 217
VpaK11BI GGWCC 1 cut(s) 94
XapI RAATTY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 231
XspI CTAG 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.