pycom12g01220

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002241
Physical Location & Seq
Reverse (-)
1050186 .. 1052194
2009 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g01220.2

Sequence Viewer

Length: 1794 bp
ATGCTACTGATCGATCTTGTCGAGCACGGAAGTCAATCCTCTGAAGACGTAGATTCTCTGGAAATGGAACAGGCCGGAAAAAGAGATGTTCAACATAATGAGCTTGAAATAAAATTGGTTGAATCGCAAGACCTGGAACCAAGCTTGTCTGGTGCAACTGGTTTGGCTACTCATGTGGAACATGTTAACGAAGATCATTTCAAACCAGAACCGATTGAAGAGGATAACAGCAGTACGTCAAGCTTGTCATCATTGACAGAAGTGGATGAAAAGATTGCAGATGTAGTGAAACGTGGATCAACCAGTTTGGAGGCAAGAGGTGATATCACCAAGGGGTTTGTAATTTCGCCACAACCTTCACTGAAGGAGTCAGAAGTCCAGTTTATGAGCAGGACACTGAATGAAGATGAACACGATGAGCCAGTCTATGACTCAACCGCCCTACCAACTGAAAAGATCCTTTCCTTTAACTCTATTTCTTCTGATATACAAGCAGATGTATCCGAAATGGTTACACCTCCAGCATTAGCTGAAGTGCAAGTTCCGTTTGTAGACCGGCGTTGTGAAGTGTATGGTGAGAGCACAGACAAAGATACTTCAGGTTTTGTAGAGACAAGCGGTGCCACCTCAAAAGTACATGCATCAGATGAAATTGAAAGAAGCTTGGGGACAGGCAACCAAGTCGGCTTAGCGGTTTCACATTCAGAGGACGATATCTCTCTCCCTAAGATTTTTGACATGAAGACAGCTGATTGTAGTTATCAATCTGTTCTTTCTGAAGAACAGCCGTCATCAGAGCTGGATAAAGTCCTTTCGTGGTCAGATAAATCCATGGTTGAACCATATCTTGATGATCATGTGGAAGCATTTATTATTAAAGAAGAAGTAGATGACATAAAGGAGATTGATGCAGGGTTGCTGTCGGAATTGGATGCAGTTGGGGACTTCAGTACGAATGAAGTTGGTGAGGCACTCCATACAGAGGAGCTGATTCCAGAGGAAGCAAATGTTTTAAGCACTGAATTTGGTGATTTAAACCTGTCAGAATTGAACCAGGAGCTACCAGTTGTTGAAGCAAGATCAATTGAAAATATTGACACAGATTTTAAGCAAATGCATAAGGGACTAGCTGTTGAGGAAGTCATTCTTCCCAGTGTGGTTGACGATCAGCTAGTGGTGGAAGCATCCAAAAATACTGTCCAGACCAGTTCAGAGTTGCCAATTGTTGAAGCAAGATACTTGGAAGATTCAGAGTTGCCTACTGTTGAAGAGAGGTACTTGGAAGCATCTGAAAATACTGTCCAAACCAGTTCAGAGTTGCCAATTGTTGAAGCAAGCTACTTGGAAGATAATGTCACCGATTTAAAGCAAGATTCAGAAGGTAATGTTAATAAGCTGACGCAAGTTAATGTTGGTGAGCTTTCAAATGGGTCGGAAGAAGTAGGAACCACCGGTGTGGATTCTACGGAGGTGGTTGCATCAAGCAATACAGTATCAAGTGTTCAAGAAGATATCAATGCAGATACTGCTTTGGAGCAAGTCTTAGAAAGTCATGTTGATGAGTTGCCAAAGGTGACGTCAATTTCGAATGATGGGTCTGAAGAAGTACGATGTAATGCCATGGGTTCTTTGGATGAGATGGCATCAAGCAACATAATATCGGGCTGTCAACAAGAAAATATCACTATTCCGAAGCAAGTCTCAGACGGTCATGTTAATGATGGATCGGAAGTAGGAACTGGTGCAATAGGTTCTACGGAAGATATTGCTTCAAGCAATACATCATGTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

598

Amino Acids

64.69

Weight (kDa)

4.05

Isoelectric Point (pI)

46.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1580
AccB1I GGYRCC 1 cut(s) 620
AccI GTMKAC 1 cut(s) 552
AciI CCGC 3 cut(s) 438, 618, 692
AclWI GGATC 3 cut(s) 304, 451, 1732
AcsI RAATTY 1 cut(s) 1022
AcuI CTGAAG 7 cut(s) 63, 383, 552, 582, 798, 931, 1620
AcyI GRCGYC 1 cut(s) 1577
AfaI GTAC 5 cut(s) 235, 636, 952, 1277, 1608
AfiI CCNNNNNNNGG 1 cut(s) 982
AflIII ACRYGT 1 cut(s) 181
AgeI ACCGGT 1 cut(s) 1451
AjnI CCWGG 2 cut(s) 132, 1053
AleI CACNNNNGTG 1 cut(s) 1454
Alw21I GWGCWC 2 cut(s) 27, 584
Alw26I GTCTC 2 cut(s) 605, 1705
AlwI GGATC 3 cut(s) 304, 451, 1732
AlwNI CAGNNNCTG 1 cut(s) 1526
AoxI GGCC 1 cut(s) 72
ApoI RAATTY 1 cut(s) 1022
ArsI GACNNNNNNTTYG 8 cut(s) 1182, 1214, 1562, 1567, 1580, 1594, 1599, 1612
AsiGI ACCGGT 1 cut(s) 1451
Asp700I GAANNNNTTC 1 cut(s) 1143
AsuHPI GGTGA 8 cut(s) 319, 332, 587, 977, 1040, 1348, 1427, 1585
AsuII TTCGAA 1 cut(s) 1586
BanI GGYRCC 1 cut(s) 620
BbsI GAAGAC 2 cut(s) 51, 749
Bbv12I GWGCWC 2 cut(s) 27, 584
BccI CCATC 3 cut(s) 1586, 1633, 1715
BceAI ACGGC 1 cut(s) 772
BcgI CGANNNNNNTGC 2 cut(s) 664, 698
BciT130I CCWGG 2 cut(s) 134, 1055
BciVI GTATCC 1 cut(s) 511
BclI TGATCA 1 cut(s) 853
BcoDI GTCTC 2 cut(s) 605, 1705
BfaI CTAG 2 cut(s) 1127, 1172
BfuI GTATCC 1 cut(s) 511
BlpI GCTNAGC 1 cut(s) 688
Bme1390I CCNGG 2 cut(s) 134, 1055
BmiI GGNNCC 3 cut(s) 138, 622, 1447
BmrFI CCNGG 2 cut(s) 134, 1055
BmrI ACTGGG 1 cut(s) 1146
BmsI GCATC 7 cut(s) 650, 898, 922, 1193, 1295, 1487, 1652
BmuI ACTGGG 1 cut(s) 1146
BpiI GAAGAC 2 cut(s) 51, 749
BpmI CTGGAG 1 cut(s) 504
Bpu1102I GCTNAGC 1 cut(s) 688
Bpu14I TTCGAA 1 cut(s) 1586
Bsa29I ATCGAT 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 1577
BsaJI CCNNGG 3 cut(s) 330, 831, 1620
BsaWI WCCGGW 1 cut(s) 1451
Bsc4I CCNNNNNNNGG 1 cut(s) 982
Bse118I RCCGGY 2 cut(s) 555, 1451
Bse1I ACTGG 9 cut(s) 163, 303, 379, 422, 1064, 1152, 1206, 1308, 1744
BseBI CCWGG 2 cut(s) 134, 1055
BseCI ATCGAT 1 cut(s) 12
BseDI CCNNGG 3 cut(s) 330, 831, 1620
BseGI GGATG 4 cut(s) 271, 937, 1184, 1639
BseLI CCNNNNNNNGG 1 cut(s) 982
BseMII CTCAG 1 cut(s) 1716
BseNI ACTGG 9 cut(s) 163, 303, 379, 422, 1064, 1152, 1206, 1308, 1744
BseRI GAGGAG 1 cut(s) 998
BshFI GGCC 1 cut(s) 74
BshNI GGYRCC 1 cut(s) 620
BshTI ACCGGT 1 cut(s) 1451
BshVI ATCGAT 1 cut(s) 12
BsiHKAI GWGCWC 2 cut(s) 27, 584
BsiSI CCGG 3 cut(s) 75, 556, 1452
BslFI GGGAC 3 cut(s) 682, 956, 1137
BslI CCNNNNNNNGG 1 cut(s) 982
BsmAI GTCTC 2 cut(s) 605, 1705
BsmFI GGGAC 3 cut(s) 682, 956, 1137
BsnI GGCC 1 cut(s) 74
Bsp119I TTCGAA 1 cut(s) 1586
Bsp1286I GDGCHC 2 cut(s) 27, 584
Bsp143I GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
Bsp1720I GCTNAGC 1 cut(s) 688
Bsp19I CCATGG 2 cut(s) 831, 1620
BspACI CCGC 3 cut(s) 438, 618, 692
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 1 cut(s) 1715
BspDI ATCGAT 1 cut(s) 12
BspLI GGNNCC 3 cut(s) 138, 622, 1447
BspPI GGATC 3 cut(s) 304, 451, 1732
BspT104I TTCGAA 1 cut(s) 1586
BspT107I GGYRCC 1 cut(s) 620
BsrFI RCCGGY 2 cut(s) 555, 1451
BsrI ACTGG 9 cut(s) 163, 303, 379, 422, 1064, 1152, 1206, 1308, 1744
BssAI RCCGGY 2 cut(s) 555, 1451
BssECI CCNNGG 3 cut(s) 330, 831, 1620
BssMI GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
BssNI GRCGYC 1 cut(s) 1577
BssT1I CCWWGG 3 cut(s) 330, 831, 1620
Bst2UI CCWGG 2 cut(s) 134, 1055
Bst4CI ACNGT 5 cut(s) 1198, 1264, 1300, 1492, 1709
Bst6I CTCTTC 2 cut(s) 213, 1263
BstACI GRCGYC 1 cut(s) 1577
BstAPI GCANNNNNTGC 1 cut(s) 1526
BstBI TTCGAA 1 cut(s) 1586
BstC8I GCNNGC 1 cut(s) 1336
BstDEI CTNAG 4 cut(s) 688, 726, 1543, 1702
BstDSI CCRYGG 2 cut(s) 831, 1620
BstF5I GGATG 4 cut(s) 271, 937, 1184, 1639
BstKTI GATC 9 cut(s) 12, 16, 196, 299, 459, 856, 1082, 1168, 1727
BstMAI GTCTC 2 cut(s) 605, 1705
BstMBI GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
BstMWI GCNNNNNNNGC 1 cut(s) 1526
BstNI CCWGG 2 cut(s) 134, 1055
BstNSI RCATGY 2 cut(s) 185, 641
BstSCI CCNGG 2 cut(s) 132, 1053
BstV2I GAAGAC 2 cut(s) 51, 749
BstX2I RGATCY 1 cut(s) 456
BstXI CCANNNNNNTGG 1 cut(s) 1456
BstYI RGATCY 1 cut(s) 456
Bsu15I ATCGAT 1 cut(s) 12
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 1 cut(s) 74
BsuTUI ATCGAT 1 cut(s) 12
BtgI CCRYGG 2 cut(s) 831, 1620
BtsCI GGATG 4 cut(s) 271, 937, 1184, 1639
BtsIMutI CAGTG 4 cut(s) 359, 395, 1017, 1159
Cac8I GCNNGC 1 cut(s) 1336
CaiI CAGNNNCTG 1 cut(s) 1526
Cfr10I RCCGGY 2 cut(s) 555, 1451
ClaI ATCGAT 1 cut(s) 12
CseI GACGC 1 cut(s) 1408
Csp6I GTAC 5 cut(s) 234, 635, 951, 1276, 1607
CspAI ACCGGT 1 cut(s) 1451
CviQI GTAC 5 cut(s) 234, 635, 951, 1276, 1607
DdeI CTNAG 4 cut(s) 688, 726, 1543, 1702
DpnI GATC 9 cut(s) 11, 15, 195, 298, 458, 855, 1081, 1167, 1726
DpnII GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
DraI TTTAAA 2 cut(s) 1035, 1365
Eam1104I CTCTTC 2 cut(s) 213, 1263
EarI CTCTTC 2 cut(s) 213, 1263
Eco130I CCWWGG 3 cut(s) 330, 831, 1620
Eco32I GATATC 3 cut(s) 325, 715, 1513
Eco57I CTGAAG 7 cut(s) 63, 383, 552, 582, 798, 931, 1620
EcoRII CCWGG 2 cut(s) 132, 1053
EcoRV GATATC 3 cut(s) 325, 715, 1513
EcoT14I CCWWGG 3 cut(s) 330, 831, 1620
EcoT22I ATGCAT 2 cut(s) 643, 1119
ErhI CCWWGG 3 cut(s) 330, 831, 1620
FalI AAGNNNNNCTT 2 cut(s) 1131, 1163
FaqI GGGAC 3 cut(s) 682, 956, 1137
FbaI TGATCA 1 cut(s) 853
FblI GTMKAC 1 cut(s) 552
FokI GGATG 4 cut(s) 278, 944, 1171, 1646
FspBI CTAG 2 cut(s) 1127, 1172
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 74
HapII CCGG 3 cut(s) 75, 556, 1452
HgaI GACGC 1 cut(s) 1408
Hin1I GRCGYC 1 cut(s) 1577
HincII GTYRAC 3 cut(s) 187, 1162, 1670
HindII GTYRAC 3 cut(s) 187, 1162, 1670
HindIII AAGCTT 3 cut(s) 142, 241, 661
HinfI GANTC 8 cut(s) 53, 122, 368, 431, 991, 1247, 1373, 1460
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 3 cut(s) 75, 556, 1452
HphI GGTGA 8 cut(s) 319, 332, 587, 977, 1040, 1348, 1427, 1585
Hpy166II GTNNAC 4 cut(s) 187, 553, 1162, 1670
Hpy188III TCNNGA 5 cut(s) 59, 848, 995, 1201, 1505
Hpy8I GTNNAC 4 cut(s) 187, 553, 1162, 1670
HpyAV CCTTC 3 cut(s) 358, 366, 1373
HpyCH4III ACNGT 5 cut(s) 1198, 1264, 1300, 1492, 1709
HpyCH4IV ACGT 4 cut(s) 48, 236, 292, 1577
HpyF10VI GCNNNNNNNGC 1 cut(s) 1526
HpyF3I CTNAG 4 cut(s) 688, 726, 1543, 1702
HpySE526I ACGT 4 cut(s) 48, 236, 292, 1577
Hsp92I GRCGYC 1 cut(s) 1577
Ksp22I TGATCA 1 cut(s) 853
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
LmnI GCTCC 3 cut(s) 985, 1057, 1534
LweI GCATC 7 cut(s) 650, 898, 922, 1193, 1295, 1487, 1652
MaeI CTAG 2 cut(s) 1127, 1172
MaeII ACGT 4 cut(s) 48, 236, 292, 1577
MaeIII GTNAC 4 cut(s) 511, 1354, 1573, 1787
MalI GATC 9 cut(s) 11, 15, 195, 298, 458, 855, 1081, 1167, 1726
MboI GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
MfeI CAATTG 3 cut(s) 1083, 1221, 1323
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 2 cut(s) 27, 584
MlyI GAGTC 2 cut(s) 377, 425
MmeI TCCRAC 2 cut(s) 903, 1413
Mph1103I ATGCAT 2 cut(s) 643, 1119
MroXI GAANNNNTTC 1 cut(s) 1143
MslI CAYNNNNRTG 3 cut(s) 1454, 1557, 1716
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 3 cut(s) 75, 556, 1452
MspR9I CCNGG 2 cut(s) 134, 1055
MunI CAATTG 3 cut(s) 1083, 1221, 1323
MvaI CCWGG 2 cut(s) 134, 1055
MwoI GCNNNNNNNGC 1 cut(s) 1526
NcoI CCATGG 2 cut(s) 831, 1620
NdeII GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
NlaIV GGNNCC 3 cut(s) 138, 622, 1447
NmuCI GTSAC 2 cut(s) 1354, 1573
NsiI ATGCAT 2 cut(s) 643, 1119
NspI RCATGY 2 cut(s) 185, 641
NspV TTCGAA 1 cut(s) 1586
OliI CACNNNNGTG 1 cut(s) 1454
PciI ACATGT 1 cut(s) 181
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 1583
PdmI GAANNNNTTC 1 cut(s) 1143
PfeI GAWTC 6 cut(s) 53, 122, 991, 1247, 1373, 1460
PinAI ACCGGT 1 cut(s) 1451
PleI GAGTC 2 cut(s) 376, 425
PpsI GAGTC 2 cut(s) 376, 425
PscI ACATGT 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 132, 1053
PspGI CCWGG 2 cut(s) 132, 1053
PspN4I GGNNCC 3 cut(s) 138, 622, 1447
PsrI GAACNNNNNNTAC 2 cut(s) 1485, 1517
PstNI CAGNNNCTG 1 cut(s) 1526
PsuI RGATCY 1 cut(s) 456
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 5 cut(s) 235, 636, 952, 1277, 1608
RsaNI GTAC 5 cut(s) 234, 635, 951, 1276, 1607
RseI CAYNNNNRTG 3 cut(s) 1454, 1557, 1716
Sau3AI GATC 9 cut(s) 9, 13, 193, 296, 456, 853, 1079, 1165, 1724
SchI GAGTC 2 cut(s) 377, 425
ScrFI CCNGG 2 cut(s) 134, 1055
SduI GDGCHC 2 cut(s) 27, 584
SfaNI GCATC 7 cut(s) 650, 898, 922, 1193, 1295, 1487, 1652
SfuI TTCGAA 1 cut(s) 1586
SgrAI CRCCGGYG 1 cut(s) 1451
SmiMI CAYNNNNRTG 3 cut(s) 1454, 1557, 1716
SsiI CCGC 3 cut(s) 438, 618, 692
SspI AATATT 1 cut(s) 1093
SspMI CTAG 2 cut(s) 1127, 1172
StyD4I CCNGG 2 cut(s) 132, 1053
StyI CCWWGG 3 cut(s) 330, 831, 1620
TaaI ACNGT 5 cut(s) 1198, 1264, 1300, 1492, 1709
TaiI ACGT 4 cut(s) 51, 239, 295, 1580
TaqI TCGA 3 cut(s) 12, 21, 1586
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 6 cut(s) 53, 122, 991, 1247, 1373, 1460
TscAI CASTG 4 cut(s) 366, 402, 1024, 1159
TseFI GTSAC 2 cut(s) 1354, 1573
Tsp45I GTSAC 2 cut(s) 1354, 1573
TspDTI ATGAA 6 cut(s) 282, 417, 423, 663, 755, 972
TspGWI ACGGA 4 cut(s) 42, 534, 1481, 1772
TspRI CASTG 4 cut(s) 366, 402, 1024, 1159
XapI RAATTY 1 cut(s) 1022
XceI RCATGY 2 cut(s) 185, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 1627
XmiI GTMKAC 1 cut(s) 552
XmnI GAANNNNTTC 1 cut(s) 1143
XspI CTAG 2 cut(s) 1127, 1172
ZraI GACGTC 1 cut(s) 1578
Zsp2I ATGCAT 2 cut(s) 643, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.