Rh6DG135200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002241
Physical Location & Seq
Forward (+)
19328892 .. 19340180
11289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG135200.1

Sequence Viewer

Length: 7044 bp
ATGCTGGCTTCAGTTCACCATCATCCTGCCTTTGCAAGCTCCTTCCTGTTCCCCTGCTCACATGGGTATCCTAGTCCAAGAAGGCAATCTCCACCAACTGGGTTGCACTTGAACAGAGCTCTGATCAAACTGAGAAGACCAAGAGGTCCAAGGCCAATGACTTTTGGGAGCAATGGAGGTGAACCTGAAGTTGGGGAGCGGCTTTATCTGGGACTGGACTTTGGGACTTCTGGAGCGAGGTTTGCCCTTATCGATAAACGAGGGATAATTCATGCTGAGGGAAAGAGAGTATACCCTCCTTTTATGAGTGAAGAAAAAATGGATTGGGCCCATTCATGGAAGGCCACACTCTTCTCACTTCTTGGAGACATTCCATCTCATCTTCGTAAACTGATTGCTTCCATTTCCATTGATGGAACTTCTGCAACTACTCTCATTGTTGACAGCAACACAGGAGAGCCGTTATGGAGACCTTTCCTCTACAATGAGAGCTGCCCTGATGCTTTACCGACGGTTAAATCCATTGCTCCTCCATACCATACAGTCTGCTCTGGTTCCTCTACTCTGTGCAAGCTTGTCTCCTGGTGGGAGAATGATGATCCAAATAAAAAATCTGCACTACTGTTGCACCAAGCAGATTGGCTTTTGTGGCTTCTTCATGGAAAACTCGGAGTGTCTGATTATAATAATGCTCTGAAGGTTGGTTATGATCCTGAGCTTGAATCCTATCCACCCTGGCTACTCTCTCAGCCCTATTCACAACTTTTACCTTCTGTTAGAGCTCCTGGAACTTCAATCGGTCATTTGAAAGAGGACATAAGATCGGATTTTGGTTTTCCAAGTGATTGTGCTGTATGCACAGGAACCACTGATAGTATAGCAGCTTTTTTAGCAGCTCGTGCAACACAACCTGGGAAAGCTGTAACTTCTTTGGGTTCAACACTTGCCATAAAACTGCTGAGCACTACTAGGATTGAGGATGCACGGTTTGGGGTGTATAGTCATCGCCTTGATGATAAGTGGCTTGTTGGAGGTGCTTCAAATACTGGTGGAGCAGTTCTTAGACAAATTTTCACTGACGAGCAACTGGAGAAATTGAGTGAACAGATTGATCCCATGGAAGCTTCTCCTCTAGACTACTACCCACTGCAATCAGTTGGTGAGAGATTTCCAGTGGCAGATCCGAATATGGCTCCAAGATTACACCCACGTCCAGAAAGTGAGGTGGAGTTTTTGCATGGGATTCTTGAATCCATTGCGCGTATAGAGGCAAAAGCTTACAACTTACTCAAGGAACTAGGCGCAACCCAAGTTGATGAAGTGTTCACAGCCGGAGGTGGTGCGAGAAATGAGAAATGGACAAAGATTAGAGAGAGAGTACTTGGTTTGCCTGAACACATTGCTTTTTCTTTCTCTCTTCTGGTCTTGGATTCATCACGATCACCATCTGAAATTTTAGAATTTTATTTTAATTCAACTTTTGTGATTGGAGCTGAAATCAATATCTGTATCCATTATCCAGAGGACATGGGGTTTGTGTTAGAAATTGGAGTTCAAATCAGGAAATCTGTGACCACATCACTCAGAACATGTTACAGATCACTTTACCATCATCCATTTCTCGTGGCATTTCTGCTTTTCTTGACATTTCTGCAGAGATTCTTTCCCTTTGCCTTTTCTATTTTGGTGACTGCATCCCCTGTTTTGGTCTGCTCTGTTGTTCTTCTTGGGACCCTTTTGATTTTCGGCCAGCTAGAATTACCTGAAACCAAAAGGCAAAAGAAGATTACCCATGATTTTGCTTCTCTTAGAACCCGGGTTTCAGCGGATGAGGCTGTTACTTTGGAGAGGAATGGGAGGTTTTCTATACATAAGTTCTCAGGAAAGAGTTTAGATGAAGTGAGTTGCAGTAGATACAGTAGTGATCGTGAAGGTTCAATTGGTTATGTGCCTCTTATTGATGAGACATTTCATTACGCCGAGAGTGAGAAGAGAGAATTAAATAGTAAGGAGCTGGAGAACCAGAGAGTCATTCATAAGCAGAAATTCGGGATTGATGCCATGACAAGGAATGTGGAAGCTATTGAGAAGCCAGCTTCCCTCAAAGGTGATCACTCGGAGTCTTCACAGGGTGGCGGTGGTGGTGATGATGAGGCTTCAGATTCTGGCTCAGACCAAGCAGAGAGTTCGTCACCAGATGCTTCAATAGCAGACATCCTTCCAATTCTTGATGAGCTACACCCACTTTTAGACTTAGATGCTCCACTACCTGCTCATATGTCCCCTGATGATTCTGGGGTTGGGTCTGATAGGAGTAATGATGGCAGTAATGAGTCAGATGATGAGGATACTGAAATCCGGGATCGAGGAGTAGAAGAGTATGGGGTTGATGATAATGATGGCGATGAGGAAGAAGCACATGATGGTCAAGAGGATGAAAGTAAATCTGCAATCAAGTGGACAGAGGATGACCAAAAGAATCTCAGGGACTTGGGGAATTTGGAGCTTGAAAGGAACCGATGCTTGGAGAACCTTATTACAAGGAGAAGTGCATCGAAAAGACAGAACCCTGAGAAGAATCTGATAGATTTAGATACTTCTGAAAATCCCTTTAATATAACACAAATTTCGACAACAAGACACAACCCTTTTGATCTCTCACATGATTCCTATGATGATATGGGGTTGCCACCCATTCCTGGTTCTGCTCCATCTACTTACTTACCAGGAAGAAACCCCTTTGATCTTCTGTATGAGCCAAATGAAGAAAATCCTGAGCTCAAGGGAGATCATGTTGAGCAAGAATTTGCGACAGTTGTCCCCAATAATACATTCTTTTGCAGGCATGCAAGCATTAGACCATCTAGGTTAGGGGATGCCAGGCAAGAGAGGCAAGATCTTAAATGGAGACCTGTTTTTGTACCAGAACAGTTGGCTTCAGAAGAAACAAGCTATTGCTCATTCCAAAGATTACCAAGAGAAGCTAGTGATTCCAAGTTGAGTTCCATTCCTGATATTGAGTCAGTGAGTTCCGGAGAAGATCTGGATGAAAGGAAGTTCAATGAGCGCGTTAAAGAAGCAGAAGTGATCTCCAATATATACCAAGCTTCTGAGCTTGTTGACCATAGAAGGCATTCTTCTGAAGATCTAGATCATCTGGAAATGGAACGGCCTGGGAAAAAAGATGTCCAGCATGATGAGCCGGAAATAAAATTGGGCCAGGTGGAGAATGGGAATGCAAGCTTGTCTGGTACAGGAGGATTTGTTATTCCTGTGGAACCTATTACTAATGCAACTTATTTGAAACCAGAAGCATTTGAAGATAAAAACAGCAGTAGGTCAAGCTTGTCATCGTCATCAGAAGCAGATGAAAAATCTTCAGATTTGAGGAAAGATGGATCAAAATTTTTTGAGCCATTTGAGTCAGAAGTGCAAACTACGAGTGGAATGCTGGATGACAATCAACATAAGGAGCCAGTCTATGACACAAGCCCCCCAGCATCTGCAAAGGTCCTTTCCTTTAATTCCATTTCTTCTGATATTCAAGCAGAAATGTCTGAAGTGGTTACACTTCCATCATTGGCTGAAATGCGTGTTCCCTCTGTAGACCAAGCCACAGATTCACACTTTCATGGTAAAATCATAGAAGAAGGTACTTCAGGTAATGAACAGATTAGTGCCACCTCACATGTACGTGCATCAGTTGAAAATGGAAGAGATCCTGGGGAATGCAACCAGCTCTTCTTAGCAGCTTCTCATTCAGATGTAAATGATGATCTCCCTAAGAATTTGAATGTGAAAGCAGCTTTTACAGACTCTAGTCCTCAGTATGTTTCTTCGAAAGATAAAATGCCAGCAGAGGAGAAAAACTTATTCTTGTCCGATATGCCATCTTTTCATGATCATTTTGCATTTCCTGAACCACGTATCATCTTATCAGAGTCAAAAGAAGATTCAAGCATCAAGGACTTAAATGCAGTTGGAGTCCCAGACCCCCCTGTGGGTGTAAAATCTACCTCAAGCAAGGTGATAGAATCAAGCAACGAAGCATCTGGTGTTCAACAAACTATCAGTACTCATATTGCTATGGAGCAAGTCTCAGAAGCTAATGAGCTGCCAAAGCCATCAAATTCAAAAGATGGGTCAGTGGAAGTAGGAACTACTGCAGTGGATTCTACAAAGGAGATTGCATCAAGCAATGCAGGAACAAGTATTCAAGGAACTGTCACGCCTCATGTTTCTCCAAAGCAATTCTCAGAAGCTAATGTTGGTGAGCAGACACAACCATCAAATTCAAAAGATAAATCGTCAAAAGTGGAAACTAGTACAAAGGGTTCTACCAAGGAGATTGCATCAAGCAACACAGAAACTGGTGTTAAAGAACCTATTGCTACTCCTAATTCTCCAAAGGAAGTCTCAAAAACTGGTGTCAAAGAACCGGTCACTACTCCTAATTCTCCAAAGAAAGTCTCAGAAAATAATGATCCTGTGCTGCCTATTTCATCAAAATTCAAGGATGGGTCAACAGAATTAGGAACCAAAACTTCTCCGAAGAAAGTCTCAGAAGCTAATGATCGCGAGGTGCCTAAACCATCAAATTCAAAGGATGGGTCGACAGAATTTGGAACCAATGCAATGGGCACTACCAAGGAGATTACATCAATTAATATAGGAACTGCTGTTCAGGAAACAGTTACCACTGATCATGCTCCTAAGGAGGTTTCAGAAGATAATGTTGGTGAGCTGCCAAAACTATCAAATCCAAAAGATGGATCTACAGACGTTGGAAATAATACAGTGGGTTCTACCAAGGACATTGCACTAAGCAGTACAGGATCTAGTGTTCAAGAAACTGCTGAAACTCCTCTGAAGCAAGTCTCAGAAAGTGATGTCAGTAAGCTGCCAAAATTGTCAAAGGACATTGCACTAAGCAGTACAGGATCTGGTGTTCAAGAAACTGCTGAAACTCCTCTGAAGCAAGTCTCAGAAAGTGATGTCTGTAAGCTGCCAAAATTGTCAAAGGACATTGCATTAAGCAATACTGGATCTGGTGTTCAAGAAACTGCCACTACTCCTGTGAAAGAAGTCTCAAAAACTGATGTCAGTGAGTTGCCAAAATCGTCAAAGGATGAACTAGCAAAAGTAGCAACTAATGATGCAGTTGGTTCTACCACGAGTCTTCAAGAAACTGCCCCTACTCTTCCGAAGCAAATCTCCAAAGGTGATCTCAGTGAGTTGCCAAAATCATCAAATTCAAAGGATGGATCGAATAAAATAGAAAGTAATGCAGTGGGTTCTGCCAAGGAGATTGCATCAAATACTATAGGAACTGGTGTTCAAGAAACTGTCTCTACTCAAACTCCTGTAAAGGAATTATTAGAAGTTAATGTTGGAGACCAGCCAAAACCATCAGATTCAAAAGATGAACATAAAGAAATAGAAATTAATGCAGTGAGTTTTACAAAGGAGACTGCATCAAAAAATGCCAGATCTGGTGTAAAAGAAACAGTTACTACTGACCAAAGGAAAGTCTCAGAAGGTAATTTAATTGAGCTGCCAAAAACGTTGAATTCAAAGGATGAATCATTGGAAGTAGCAACTAGTAATGCAGTGGGTTCTACTAAGAATGTACAAGAGATTGCCAGTAATCCTTTGGAAGAAGTTTCAGAAGGTAATGTCTTTGAGCTGCCAAAACCTTCAAACTCAAAGGATGGATCACCAGGAGTAACTAATGCAGTGAATTCTACCAACAGTGTTCAAGATACTAACGTCGGTGAGCTTCCAAAACCTTCAAATGCAAAGGATGGATTGACAGAAGTAAGAACTAATGCAGTGGATTCTACCAAGAAGCTTGCATCAAGCAACACAGGAACTTGTGTGCAAGAAGGTAACACCGCTCATACTGCTAAACAAGTGTCAGAAGGTAATCTCTCTGAGCTGCCAACACCATCACATTTAAATGACGGATCAGCAGAAGTAGGAACTAATGCAGTGGGTTCTACCAAGAACGTTGAAGAAACTGCTACTCCGAAGCAAGTTTCAGAAGGTAATATCAACGAGCTGCTAAAACCTTCAGACTTAAAGGATGGATCACCAGAAGTAACTGGTGCAGTGGATTCTACCAAGAGTGTTCAAGATACTAACGTCGGTGAGCTCCCAAAACCTTCAAATGCAAAGGATGGATCGACAGAAGTAATAACTAATGCAGTTGATTCTACCAAGAAGCTTGCATCAACCAACATGGAAAGTAATTTGCAAGAAGGTATCACTGCTCATACTGCTCAACAAGTGTCAAAAGGTAGTCTCTCTGAGCTGCCGACACCATCACATTTAAATGACAGATCAGCAGAACAAGGAACTAATGCAGTGGGTTCTACCAAGAGTGTTGGAGAAACCGTAACTACTCTGAAGCAAGTTTCAGAAGGTAATATCAACAAGCTGCCAAAACCTTTAGACTTAAAGGATGGATCGACCAAAGTGCCTAATACAGTCAGTTCTACCAAGAGTGTTCAAGAAACTGTCACTATAAAAAAAGTTTCAGAAGGTAATGACGGTGAGCTTCAAAAGCCTTCAAAGGATGGATCAACAGAAGTAGGAACTAATGTAGTTGATTCTACCAAGAAACTTGGAACTAGTGTACAAGAAAGTATCACTGCTCATACTGAAAATAAAGGTGCAGAAGGTGGTGTTGGTGAGCTTCCCAAATCATCAAATTTAAAGGATGAATTACCAAAAGTAGGAGTTGATGCAGTCGGTTCCTCCAAGGACATTCCATCGAGCAACACAGAATCTGGTGTTCAAGAAAGTATCACTACTTCAGAAGGTGATGTTGCTGAGTTGCCAAGACCATCGAATTCGGATGGTCATGTTGGTGAGGTTCTAAAACCATCTATTTCAGAAGAAAGTAATGCGGGTGAATTGACAAAACCAACAAATCCAAAGGAAAGTAATGCGGGTGAGTCGCAAAAACCACCAGATCTAAAGGAAAGTAATGCTGGTGAGTCGCAAAAAACACCAGAATCAAAAGGATGGTTATGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2347

Amino Acids

253.07

Weight (kDa)

4.92

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FGGY_N PF00370 68 - 297 1.2e-07 FGGY family of carbohydrate kinases, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 684
AasI GACNNNNNNGTC 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 2278
AccB1I GGYRCC 1 cut(s) 4582
AccB7I CCANNNNNTGG 2 cut(s) 98, 4769
AccBSI CCGCTC 2 cut(s) 199, 5897
AccI GTMKAC 3 cut(s) 291, 3606, 4613
AccII CGCG 3 cut(s) 1261, 3069, 4578
AccIII TCCGGA 1 cut(s) 3032
AciI CCGC 6 cut(s) 199, 1826, 2136, 5895, 6911, 6953
AclI AACGTT 2 cut(s) 5564, 6009
AcoI YGGCCR 1 cut(s) 1747
AdeI CACNNNGTG 1 cut(s) 2132
AflIII ACRYGT 2 cut(s) 1589, 3688
AgeI ACCGGT 1 cut(s) 4438
AhlI ACTAGT 3 cut(s) 4322, 5600, 6632
AjiI CACGTC 1 cut(s) 1211
Alw21I GWGCWC 5 cut(s) 121, 784, 965, 2781, 6156
AlwNI CAGNNNCTG 5 cut(s) 2165, 3503, 4370, 4943, 6791
Ama87I CYCGRG 1 cut(s) 1815
Aor13HI TCCGGA 1 cut(s) 3032
AoxI GGCC 6 cut(s) 152, 327, 342, 1747, 3170, 3217
ApaI GGGCCC 1 cut(s) 331
ArsI GACNNNNNNTTYG 8 cut(s) 1517, 1549, 4127, 4159, 4425, 4457, 4595, 4627
AseI ATTAAT 2 cut(s) 4665, 5445
AsiGI ACCGGT 1 cut(s) 4438
Asp700I GAANNNNTTC 1 cut(s) 3133
AspLEI GCGC 3 cut(s) 1261, 1304, 3069
AspS9I GGNCC 6 cut(s) 146, 327, 328, 1731, 3217, 3511
AsuC2I CCSGG 3 cut(s) 1816, 1817, 2360
AsuII TTCGAA 1 cut(s) 3839
AvaI CYCGRG 1 cut(s) 1815
AvaII GGWCC 3 cut(s) 146, 1731, 3511
AxyI CCTNAGG 1 cut(s) 4713
BaeGI GKGCMC 2 cut(s) 331, 4643
BaeI ACNNNNGTAYC 8 cut(s) 50, 83, 1906, 1939, 5754, 5787, 6129, 6162
BanI GGYRCC 1 cut(s) 4582
BanII GRGCYC 5 cut(s) 121, 331, 784, 2781, 6156
BarI GAAGNNNNNNTAC 2 cut(s) 6799, 6831
BauI CACGAG 3 cut(s) 897, 1622, 5173
BbsI GAAGAC 3 cut(s) 142, 2115, 5171
Bbv12I GWGCWC 5 cut(s) 121, 784, 965, 2781, 6156
BbvCI CCTCAGC 1 cut(s) 276
BceAI ACGGC 2 cut(s) 445, 3185
BcgI CGANNNNNNTGC 6 cut(s) 2169, 2203, 3430, 3464, 6460, 6494
BciVI GTATCC 3 cut(s) 78, 1520, 2341
BclI TGATCA 4 cut(s) 123, 2110, 3901, 4702
BcnI CCSGG 3 cut(s) 1816, 1817, 2360
BcuI ACTAGT 3 cut(s) 4322, 5600, 6632
BfmI CTRYAG 5 cut(s) 1652, 3603, 4164, 4776, 5322
BfuAI ACCTGC 1 cut(s) 2278
BfuI GTATCC 3 cut(s) 78, 1520, 2341
BglII AGATCT 5 cut(s) 2896, 3040, 3145, 5489, 6976
BlpI GCTNAGC 1 cut(s) 959
BmcAI AGTACT 2 cut(s) 1380, 4075
Bme18I GGWCC 3 cut(s) 146, 1731, 3511
BmeT110I CYCGRG 1 cut(s) 1815
BmgBI CACGTC 1 cut(s) 1211
BmgT120I GGNCC 6 cut(s) 146, 327, 328, 1731, 3217, 3511
BmrI ACTGGG 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 108
BoxI GACNNNNGTC 2 cut(s) 2816, 3819
BpiI GAAGAC 3 cut(s) 142, 2115, 5171
BplI GAGNNNNNCTC 2 cut(s) 4082, 4114
BpmI CTGGAG 3 cut(s) 252, 1109, 2036
Bpu10I CCTNAGC 3 cut(s) 276, 714, 2775
Bpu1102I GCTNAGC 1 cut(s) 959
Bpu14I TTCGAA 1 cut(s) 3839
BpuEI CTTGAG 3 cut(s) 1274, 2765, 4003
BpuMI CCSGG 3 cut(s) 1816, 1817, 2360
Bsa29I ATCGAT 1 cut(s) 252
BsaAI YACGTR 2 cut(s) 3695, 3926
BsaBI GATNNNNATC 3 cut(s) 1444, 3150, 3459
BsaI GGTCTC 3 cut(s) 463, 2902, 5388
BsaWI WCCGGW 2 cut(s) 3032, 4438
Bse118I RCCGGY 1 cut(s) 4438
Bse21I CCTNAGG 1 cut(s) 4713
Bse3DI GCAATG 9 cut(s) 178, 522, 1254, 1398, 4204, 4641, 4815, 4920, 5025
Bse8I GATNNNNATC 3 cut(s) 1444, 3150, 3459
BseAI TCCGGA 1 cut(s) 3032
BseCI ATCGAT 1 cut(s) 252
BseJI GATNNNNATC 3 cut(s) 1444, 3150, 3459
BseMI GCAATG 9 cut(s) 178, 522, 1254, 1398, 4204, 4641, 4815, 4920, 5025
BseRI GAGGAG 6 cut(s) 519, 1119, 2382, 3875, 4854, 4959
BseSI GKGCMC 2 cut(s) 331, 4643
BseYI CCCAGC 1 cut(s) 3496
BsgI GTGCAG 3 cut(s) 600, 6129, 6696
Bsh1236I CGCG 3 cut(s) 1261, 3069, 4578
BshFI GGCC 6 cut(s) 154, 329, 344, 1749, 3172, 3219
BshNI GGYRCC 1 cut(s) 4582
BshTI ACCGGT 1 cut(s) 4438
BshVI ATCGAT 1 cut(s) 252
BsiHKAI GWGCWC 5 cut(s) 121, 784, 965, 2781, 6156
BsiHKCI CYCGRG 1 cut(s) 1815
BsiSI CCGG 6 cut(s) 1332, 1816, 2359, 3033, 3203, 4439
BslFI GGGAC 7 cut(s) 225, 238, 1744, 2266, 2501, 2804, 3971
BsmFI GGGAC 7 cut(s) 225, 238, 1744, 2266, 2501, 2804, 3971
BsmI GAATGC 4 cut(s) 3133, 3241, 3453, 3734
BsnI GGCC 6 cut(s) 154, 329, 344, 1749, 3172, 3219
Bso31I GGTCTC 3 cut(s) 463, 2902, 5388
BsoBI CYCGRG 1 cut(s) 1815
Bsp119I TTCGAA 1 cut(s) 3839
Bsp120I GGGCCC 1 cut(s) 327
Bsp1286I GDGCHC 7 cut(s) 121, 331, 784, 965, 2781, 4643, 6156
Bsp13I TCCGGA 1 cut(s) 3032
Bsp1407I TGTACA 2 cut(s) 5629, 6637
Bsp1720I GCTNAGC 1 cut(s) 959
Bsp19I CCATGG 1 cut(s) 1116
Bsp68I TCGCGA 1 cut(s) 4578
BspACI CCGC 6 cut(s) 199, 1826, 2136, 5895, 6911, 6953
BspANI GGCC 6 cut(s) 154, 329, 344, 1749, 3172, 3219
BspDI ATCGAT 1 cut(s) 252
BspEI TCCGGA 1 cut(s) 3032
BspFNI CGCG 3 cut(s) 1261, 3069, 4578
BspHI TCATGA 1 cut(s) 3898
BspMAI CTGCAG 2 cut(s) 1656, 4168
BspMI ACCTGC 1 cut(s) 2278
BspQI GCTCTTC 1 cut(s) 3746
BspT104I TTCGAA 1 cut(s) 3839
BspT107I GGYRCC 1 cut(s) 4582
BspTNI GGTCTC 3 cut(s) 463, 2902, 5388
BsrBI CCGCTC 2 cut(s) 199, 5897
BsrDI GCAATG 9 cut(s) 178, 522, 1254, 1398, 4204, 4641, 4815, 4920, 5025
BsrFI RCCGGY 1 cut(s) 4438
BsrGI TGTACA 2 cut(s) 5629, 6637
BssAI RCCGGY 1 cut(s) 4438
BssNAI GTATAC 1 cut(s) 292
BssSI CACGAG 3 cut(s) 897, 1622, 5173
BssT1I CCWWGG 7 cut(s) 149, 1116, 4341, 4647, 4809, 5301, 6762
Bst1107I GTATAC 1 cut(s) 292
Bst2BI CACGAG 3 cut(s) 897, 1622, 5173
Bst6I CTCTTC 7 cut(s) 356, 1422, 1985, 2370, 3709, 3746, 5205
BstAPI GCANNNNNTGC 1 cut(s) 899
BstAUI TGTACA 2 cut(s) 5629, 6637
BstBAI YACGTR 2 cut(s) 3695, 3926
BstBI TTCGAA 1 cut(s) 3839
BstDSI CCRYGG 1 cut(s) 1116
BstFNI CGCG 3 cut(s) 1261, 3069, 4578
BstHHI GCGC 3 cut(s) 1261, 1304, 3069
BstNSI RCATGY 3 cut(s) 1593, 2849, 3692
BstPAI GACNNNNGTC 2 cut(s) 2816, 3819
BstSFI CTRYAG 5 cut(s) 1652, 3603, 4164, 4776, 5322
BstSLI GKGCMC 2 cut(s) 331, 4643
BstUI CGCG 3 cut(s) 1261, 3069, 4578
BstV2I GAAGAC 3 cut(s) 142, 2115, 5171
BstXI CCANNNNNNTGG 1 cut(s) 4636
BstZ17I GTATAC 1 cut(s) 292
Bsu15I ATCGAT 1 cut(s) 252
Bsu36I CCTNAGG 1 cut(s) 4713
BsuI GTATCC 3 cut(s) 78, 1520, 2341
BsuRI GGCC 6 cut(s) 154, 329, 344, 1749, 3172, 3219
BsuTUI ATCGAT 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 1116
BtgZI GCGATG 2 cut(s) 989, 2418
BtrI CACGTC 1 cut(s) 1211
BtuMI TCGCGA 1 cut(s) 4578
BveI ACCTGC 1 cut(s) 2278
CaiI CAGNNNCTG 5 cut(s) 2165, 3503, 4370, 4943, 6791
CciI TCATGA 1 cut(s) 3898
CfoI GCGC 3 cut(s) 1261, 1304, 3069
Cfr10I RCCGGY 1 cut(s) 4438
Cfr13I GGNCC 6 cut(s) 146, 327, 328, 1731, 3217, 3511
Cfr9I CCCGGG 1 cut(s) 1815
ClaI ATCGAT 1 cut(s) 252
CspAI ACCGGT 1 cut(s) 4438
CspCI CAANNNNNGTGG 2 cut(s) 2055, 2090
DraI TTTAAA 3 cut(s) 5958, 6333, 6717
DraIII CACNNNGTG 1 cut(s) 2132
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 1747
Eam1104I CTCTTC 7 cut(s) 356, 1422, 1985, 2370, 3709, 3746, 5205
EarI CTCTTC 7 cut(s) 356, 1422, 1985, 2370, 3709, 3746, 5205
Ecl136II GAGCTC 4 cut(s) 119, 782, 2779, 6154
Eco130I CCWWGG 7 cut(s) 149, 1116, 4341, 4647, 4809, 5301, 6762
Eco24I GRGCYC 5 cut(s) 121, 331, 784, 2781, 6156
Eco31I GGTCTC 3 cut(s) 463, 2902, 5388
Eco47I GGWCC 3 cut(s) 146, 1731, 3511
Eco53kI GAGCTC 4 cut(s) 119, 782, 2779, 6154
Eco81I CCTNAGG 1 cut(s) 4713
Eco88I CYCGRG 1 cut(s) 1815
EcoICRI GAGCTC 4 cut(s) 119, 782, 2779, 6154
EcoO109I RGGNCCY 2 cut(s) 1731, 3511
EcoRI GAATTC 3 cut(s) 5569, 5740, 6853
EcoT14I CCWWGG 7 cut(s) 149, 1116, 4341, 4647, 4809, 5301, 6762
EcoT38I GRGCYC 5 cut(s) 121, 331, 784, 2781, 6156
ErhI CCWWGG 7 cut(s) 149, 1116, 4341, 4647, 4809, 5301, 6762
FalI AAGNNNNNCTT 2 cut(s) 3121, 3153
FaqI GGGAC 7 cut(s) 225, 238, 1744, 2266, 2501, 2804, 3971
FauI CCCGC 2 cut(s) 6904, 6946
FauNDI CATATG 1 cut(s) 2277
FbaI TGATCA 4 cut(s) 123, 2110, 3901, 4702
FblI GTMKAC 3 cut(s) 291, 3606, 4613
FriOI GRGCYC 5 cut(s) 121, 331, 784, 2781, 6156
GlaI GCGC 3 cut(s) 1260, 1303, 3068
GsaI CCCAGC 1 cut(s) 3500
GsuI CTGGAG 3 cut(s) 252, 1109, 2036
HaeIII GGCC 6 cut(s) 154, 329, 344, 1749, 3172, 3219
HapII CCGG 6 cut(s) 1332, 1816, 2359, 3033, 3203, 4439
HhaI GCGC 3 cut(s) 1261, 1304, 3069
Hin6I GCGC 3 cut(s) 1259, 1302, 3067
HinP1I GCGC 3 cut(s) 1259, 1302, 3067
HincII GTYRAC 4 cut(s) 442, 3121, 4524, 4614
HindII GTYRAC 4 cut(s) 442, 3121, 4524, 4614
HindIII AAGCTT 8 cut(s) 572, 1122, 1275, 3105, 3241, 3343, 5849, 6224
HpaII CCGG 6 cut(s) 1332, 1816, 2359, 3033, 3203, 4439
Hpy99I CGWCG 3 cut(s) 514, 5774, 6149
HpyCH4IV ACGT 8 cut(s) 1210, 3694, 3925, 4782, 5564, 5769, 6009, 6144
HpySE526I ACGT 8 cut(s) 1210, 3694, 3925, 4782, 5564, 5769, 6009, 6144
HspAI GCGC 3 cut(s) 1259, 1302, 3067
KflI GGGWCCC 1 cut(s) 1731
Kpn2I TCCGGA 1 cut(s) 3032
Ksp22I TGATCA 4 cut(s) 123, 2110, 3901, 4702
LguI GCTCTTC 1 cut(s) 3746
MaeII ACGT 8 cut(s) 1210, 3694, 3925, 4782, 5564, 5769, 6009, 6144
MbiI CCGCTC 2 cut(s) 199, 5897
MfeI CAATTG 1 cut(s) 1938
MhlI GDGCHC 7 cut(s) 121, 331, 784, 965, 2781, 4643, 6156
MmeI TCCRAC 5 cut(s) 1009, 3961, 4765, 5371, 6367
MroI TCCGGA 1 cut(s) 3032
MroXI GAANNNNTTC 1 cut(s) 3133
MslI CAYNNNNRTG 6 cut(s) 3630, 3693, 3762, 5958, 6333, 6870
MspA1I CMGCKG 1 cut(s) 1826
MspI CCGG 6 cut(s) 1332, 1816, 2359, 3033, 3203, 4439
MunI CAATTG 1 cut(s) 1938
Mva1269I GAATGC 4 cut(s) 3133, 3241, 3453, 3734
MvnI CGCG 3 cut(s) 1261, 3069, 4578
NciI CCSGG 3 cut(s) 1816, 1817, 2360
NcoI CCATGG 1 cut(s) 1116
NdeI CATATG 1 cut(s) 2277
NmeAIII GCCGAG 1 cut(s) 2005
NmuCI GTSAC 6 cut(s) 1570, 1687, 2190, 4225, 4441, 6520
NruI TCGCGA 1 cut(s) 4578
NspI RCATGY 3 cut(s) 1593, 2849, 3692
NspV TTCGAA 1 cut(s) 3839
PaeI GCATGC 1 cut(s) 2849
PagI TCATGA 1 cut(s) 3898
PciI ACATGT 2 cut(s) 1589, 3688
PciSI GCTCTTC 1 cut(s) 3746
PctI GAATGC 4 cut(s) 3133, 3241, 3453, 3734
PdmI GAANNNNTTC 1 cut(s) 3133
PflMI CCANNNNNTGG 2 cut(s) 98, 4769
PfoI TCCNGGA 2 cut(s) 784, 2358
PinAI ACCGGT 1 cut(s) 4438
Ppu21I YACGTR 2 cut(s) 3695, 3926
PpuMI RGGWCCY 2 cut(s) 1731, 3511
PscI ACATGT 2 cut(s) 1589, 3688
PshAI GACNNNNGTC 2 cut(s) 2816, 3819
PshBI ATTAAT 2 cut(s) 4665, 5445
PsiI TTATAA 1 cut(s) 684
Psp124BI GAGCTC 4 cut(s) 121, 784, 2781, 6156
Psp1406I AACGTT 2 cut(s) 5564, 6009
Psp5II RGGWCCY 2 cut(s) 1731, 3511
PspFI CCCAGC 1 cut(s) 3496
PspOMI GGGCCC 1 cut(s) 327
PspPI GGNCC 6 cut(s) 146, 327, 328, 1731, 3217, 3511
PspPPI RGGWCCY 2 cut(s) 1731, 3511
PsrI GAACNNNNNNTAC 2 cut(s) 4318, 4350
PstI CTGCAG 2 cut(s) 1656, 4168
PstNI CAGNNNCTG 5 cut(s) 2165, 3503, 4370, 4943, 6791
RruI TCGCGA 1 cut(s) 4578
RseI CAYNNNNRTG 6 cut(s) 3630, 3693, 3762, 5958, 6333, 6870
SacI GAGCTC 4 cut(s) 121, 784, 2781, 6156
SalI GTCGAC 1 cut(s) 4612
SapI GCTCTTC 1 cut(s) 3746
Sau96I GGNCC 6 cut(s) 146, 327, 328, 1731, 3217, 3511
ScaI AGTACT 2 cut(s) 1380, 4075
SduI GDGCHC 7 cut(s) 121, 331, 784, 965, 2781, 4643, 6156
SfcI CTRYAG 5 cut(s) 1652, 3603, 4164, 4776, 5322
SfuI TTCGAA 1 cut(s) 3839
SinI GGWCC 3 cut(s) 146, 1731, 3511
SmaI CCCGGG 1 cut(s) 1817
SmiI ATTTAAAT 2 cut(s) 5958, 6333
SmiMI CAYNNNNRTG 6 cut(s) 3630, 3693, 3762, 5958, 6333, 6870
SmlI CTYRAG 3 cut(s) 1289, 2780, 4018
SmoI CTYRAG 3 cut(s) 1289, 2780, 4018
SpeI ACTAGT 3 cut(s) 4322, 5600, 6632
SphI GCATGC 1 cut(s) 2849
SsiI CCGC 6 cut(s) 199, 1826, 2136, 5895, 6911, 6953
SstI GAGCTC 4 cut(s) 121, 784, 2781, 6156
StyI CCWWGG 7 cut(s) 149, 1116, 4341, 4647, 4809, 5301, 6762
SwaI ATTTAAAT 2 cut(s) 5958, 6333
TaiI ACGT 8 cut(s) 1213, 3697, 3928, 4785, 5567, 5772, 6012, 6147
TaqII GACCGA 1 cut(s) 788
TatI WGTACW 7 cut(s) 1378, 4073, 4325, 4829, 4934, 5629, 6637
TauI GCSGC 1 cut(s) 202
TseFI GTSAC 6 cut(s) 1570, 1687, 2190, 4225, 4441, 6520
Tsp45I GTSAC 6 cut(s) 1570, 1687, 2190, 4225, 4441, 6520
TspGWI ACGGA 1 cut(s) 5979
TspMI CCCGGG 1 cut(s) 1815
Van91I CCANNNNNTGG 2 cut(s) 98, 4769
VpaK11BI GGWCC 3 cut(s) 146, 1731, 3511
VspI ATTAAT 2 cut(s) 4665, 5445
XbaI TCTAGA 2 cut(s) 1132, 3148
XceI RCATGY 3 cut(s) 1593, 2849, 3692
XcmI CCANNNNNNNNNTGG 2 cut(s) 3227, 5650
XmaI CCCGGG 1 cut(s) 1815
XmiI GTMKAC 3 cut(s) 291, 3606, 4613
XmnI GAANNNNTTC 1 cut(s) 3133
ZrmI AGTACT 2 cut(s) 1380, 4075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.