MD12G1017800.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007466
Physical Location & Seq
Forward (+)
1713631 .. 1721491
7861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1017800.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGGAAATGCCCGGTAGGAGATCCAACTACACGCTTCTGAGCCAAGTTCCCGACGACCAAGCGGCGGCGTTTTATGAGACGGAGAGCAAGAACAACAAGGGGAAAGGCGACAGAGGGTTCGATTGGGAAACCGGAGCCGATTTCCGGGCGAATCAACAACCGAATCGGGCCGGGAACCCGTACTCGTCAGCCGGGTTGCAGAGGCAGTCGAGCGGCAGCAGCTTTGCCGAGAGCTCGATTTCCGGCGAGTACTACCCTCCGACCCTATCCACCGCCGCGGTCAGTGAGATCGACGGGTTCGGGTACGTGCCGGACGATGTGTTCAAGGTTAGTGGTGGAGGGGGTGAATTTCGAATGAAAGGTGTGGATGGAGCTGTTACGGCCACTGGTGGCGGCGGCGGTTCTTCTTCAGGGAAGAGCTGGGCTCAGCAGACTGAGGAGAGTTATCAGCTGCAGCTGGCTTTGGCTCTTCGGCTTTCATCGGAGGCTACATGCGCCGATGATCCTAACTTCTTGGATCCAGTGCCGGATGAATCGGCGTCCAGGACGTCCGGCTCTGTTGATGCCGTTTCGCATCGGTTTTGGGTGAATGGCTGTCTGTCATACTTTGACAAAGTGCCTGATGGATTTTACCTAATTCATGGGATAGATCAGTATGTATGGACTGTGTGCACTGATCTGCAAGAAAATGGTCGTATACCATCAATTGAATCATTAAGGTCTGTTGATCCTGGCATTGGTTCAAGCATTGAAGTGGTTTTAATTGATCGACGTAGTGATCCCAGTCTAAAGGAGCTCCAAAATAGGGTTTTAACCATTTCCTGTACTTGCATAAATACAAAAGAGATTGTAGATCATCTGGCAAAGCTTGTGTGCAGTCGCATGGGGGGTTCATCTTCCGTAGGAGAGGATGAAATTTTGTCCATATGGAGGGAGAGCAGCGATGATCTGAAAGATTGCTTAGGATCTGTAGTTGTTCCAATAGGCAGCCTTTCTGTTGGCTTATGTAGACATCGTGCCTTATTATTCAAAGTTCTAGCTGACACAATTGATTTACCTTGTCGAATTGCCAAAGGCTGTCAATATTGTACAAGGGATGACGCCTCCTCTTGTCTTGTCCGGTTTGGGTTGGACAGGGAGTATTTAGTCGATTTGATTGCAAATCCAGGCTACTTGCGCGAGCCTGATTCCTTGCTCAATGGCCCATCATCCATCTCAATTTCTTCACCTCTGCGCTTTCCAAGACTAAAACCAGTTGAACCTACCATTGATTTCAGGTTACTGGCTAAACAGTATTTCTCAGACTGTCAATCGCTTAATCTTGTGTTTGATGAAGCTCCTGCAGGAAGTGCTGTTGATGAGGACAATATACATCCTAAAAAATTTGATAGGAAGAGTACAGAGGGAAAAAACCTAATTTCAAATCTCAGCGGTGACACCTCTGCACATGCCAAAATTCCCCGGACAAGTGGTGATGATAGAAATCCCCAGTTGTTTAATCCTCTTCAAAATATTTTGCACACACCACCCATGGTAAATGATCCAATCCCTCTAAAGTGTATGCCACCTGTAGGGCATAGAGATGGTCCAAGGGTGGATACAATTACAGACTCAAGGTTTGTTGAGGGCGTTCAACTGGTTCCTAGTAAACCAAGTAGAGAGCTCGGGTTCGATATAGAGGATTTGGACATCCCATGGAGTGAGCTCATTATAAAAGAGAGAATCGGTGCAGGTTCTTTTGGAACTGTTCATCGCGCTGATTGGCATGGCTCTGATGTTGCTGTGAAGATTCTAATGGAACAAGATTTTCATGCCGAACGCTTTAAAGAATTTTTGAGTGAGGTTACTATAATGAAGCGCCTGCGACATCCAAATATTGTTCTCTTTATGGGTGCAGTTACCAAGCCTCCAAACTTGTCCATAGTCACGGAATATTTATCAAGAGGTAGCTTGTATAGGCTTTTGCATAAAGCTGGTGCAAGGGAGGCATTGGATGAGAGGCGTCGCTTGAGTATGGCATATGATGTGGCAAAGGGAATGAATTATCTTCATAGACGCAAACCTCCCATTGTTCATCGTGATTTAAAATCTCCAAATCTGTTGGTGGACAAAAAATATACAGTGAAGGTTTGCGACTTTGGTCTTTCCCGTTTGAAGGCAAACACCTTCCTCTCCTCAAAATCAGCTGCCGGGACTCCTGAATGGATGGCACCAGAAGTCCTGCGTGATGAACCATCAAATGAGAAGTCAGATATTTACAGTTTTGGTGTAATATTGTGGGAACTCGCAACAATGCAACAGCCGTGGGGTAATTTAAACCCAGCGCAGGTTGTCGCAGCTGTTGGGTTTAAAAACAAAAGGCTTGAGATTCCACGTGATTTGAATCCTAATGTGGCAGCTATAATTGAGGCTTGTTGGGCCAACGAACCCTGGAAACGACCTTCATTTGCTAGTATCATGGATTCTTTGACGCCGTTGATCAAAGCTCCCGTCGCTCAACCTAGTCGTGCAGATATGCCATTACTTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

844

Amino Acids

92.85

Weight (kDa)

5.74

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 187 - 392 3.8e-82 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 571 - 823 5.5e-69 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 571 - 821 9.8e-55 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1712
AatII GACGTC 1 cut(s) 551
Acc36I ACCTGC 2 cut(s) 1721, 2317
AccB1I GGYRCC 1 cut(s) 2209
AccBSI CCGCTC 1 cut(s) 213
AccI GTMKAC 2 cut(s) 697, 1009
AccII CGCG 3 cut(s) 278, 1179, 1755
AclWI GGATC 8 cut(s) 15, 497, 512, 525, 722, 773, 973, 1535
AcoI YGGCCR 1 cut(s) 381
AcsI RAATTY 5 cut(s) 347, 915, 1382, 1455, 1829
AcuI CTGAAG 1 cut(s) 393
AcvI CACGTG 1 cut(s) 2375
AcyI GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
AfaI GTAC 6 cut(s) 182, 251, 305, 826, 1090, 1399
AfiI CCNNNNNNNGG 8 cut(s) 64, 144, 737, 805, 930, 1571, 2155, 2326
AjnI CCWGG 4 cut(s) 542, 730, 1165, 2429
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
Alw21I GWGCWC 5 cut(s) 236, 674, 798, 1665, 1707
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 1 cut(s) 670
AlwI GGATC 8 cut(s) 15, 497, 512, 525, 722, 773, 973, 1535
Ama87I CYCGRG 1 cut(s) 1664
AoxI GGCC 4 cut(s) 168, 381, 1201, 2418
ApaLI GTGCAC 1 cut(s) 670
ApeKI GCWGC 9 cut(s) 216, 219, 451, 454, 939, 987, 2186, 2336, 2396
ApoI RAATTY 5 cut(s) 347, 915, 1382, 1455, 1829
ArsI GACNNNNNNTTYG 8 cut(s) 101, 133, 1601, 1633, 2231, 2263, 2475, 2507
AspLEI GCGC 6 cut(s) 497, 1179, 1236, 1757, 1860, 2326
AspS9I GGNCC 4 cut(s) 168, 1202, 1586, 2418
AsuC2I CCSGG 6 cut(s) 12, 146, 172, 193, 1462, 2191
AsuHPI GGTGA 5 cut(s) 356, 598, 1218, 1445, 1484
AsuII TTCGAA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 1664
AvaII GGWCC 1 cut(s) 1586
BaeGI GKGCMC 1 cut(s) 674
BamHI GGATCC 1 cut(s) 517
BanI GGYRCC 1 cut(s) 2209
BanII GRGCYC 5 cut(s) 236, 427, 798, 1665, 1707
BbrPI CACGTG 1 cut(s) 2375
Bbv12I GWGCWC 5 cut(s) 236, 674, 798, 1665, 1707
BbvI GCAGC 9 cut(s) 228, 231, 438, 466, 951, 999, 2173, 2348, 2408
BccI CCATC 8 cut(s) 362, 617, 709, 1213, 1220, 1577, 2200, 2242
BceAI ACGGC 4 cut(s) 396, 551, 2287, 2458
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 4 cut(s) 544, 732, 1167, 2431
BciVI GTATCC 1 cut(s) 1591
BclI TGATCA 1 cut(s) 2478
BcnI CCSGG 6 cut(s) 12, 146, 172, 193, 1462, 2191
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 4 cut(s) 1037, 1644, 2451, 2502
BfmI CTRYAG 4 cut(s) 452, 969, 1341, 1569
BfoI RGCGCY 1 cut(s) 1861
BfuAI ACCTGC 2 cut(s) 1721, 2317
BfuI GTATCC 1 cut(s) 1591
BlpI GCTNAGC 1 cut(s) 426
BmcAI AGTACT 1 cut(s) 251
Bme18I GGWCC 1 cut(s) 1586
BmeT110I CYCGRG 1 cut(s) 1664
BmgT120I GGNCC 4 cut(s) 168, 1202, 1586, 2418
BmiI GGNNCC 5 cut(s) 136, 176, 519, 1641, 2211
BmrI ACTGGG 2 cut(s) 777, 1483
BmsI GCATC 2 cut(s) 553, 583
BmuI ACTGGG 2 cut(s) 777, 1483
BoxI GACNNNNGTC 1 cut(s) 2139
BplI GAGNNNNNCTC 2 cut(s) 409, 441
Bpu10I CCTNAGC 1 cut(s) 961
Bpu1102I GCTNAGC 1 cut(s) 426
Bpu14I TTCGAA 1 cut(s) 352
BpuEI CTTGAG 3 cut(s) 1597, 2029, 2384
BpuMI CCSGG 6 cut(s) 12, 146, 172, 193, 1462, 2191
BsaAI YACGTR 2 cut(s) 307, 2375
BsaBI GATNNNNATC 2 cut(s) 1482, 2382
BsaHI GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
BsaJI CCNNGG 7 cut(s) 276, 1460, 1530, 1589, 1694, 2303, 2429
BsaWI WCCGGW 2 cut(s) 131, 1119
BsaXI ACNNNNNCTCC 2 cut(s) 1891, 1921
Bsc4I CCNNNNNNNGG 8 cut(s) 64, 144, 737, 805, 930, 1571, 2155, 2326
Bse1I ACTGG 7 cut(s) 391, 521, 783, 1253, 1287, 1489, 1641
Bse8I GATNNNNATC 2 cut(s) 1482, 2382
BseBI CCWGG 4 cut(s) 544, 732, 1167, 2431
BseDI CCNNGG 7 cut(s) 276, 1460, 1530, 1589, 1694, 2303, 2429
BseJI GATNNNNATC 2 cut(s) 1482, 2382
BseLI CCNNNNNNNGG 8 cut(s) 64, 144, 737, 805, 930, 1571, 2155, 2326
BseMII CTCAG 5 cut(s) 29, 426, 440, 1314, 1441
BseNI ACTGG 7 cut(s) 391, 521, 783, 1253, 1287, 1489, 1641
BseRI GAGGAG 3 cut(s) 452, 1096, 2164
BseSI GKGCMC 1 cut(s) 674
BseXI GCAGC 9 cut(s) 228, 231, 438, 466, 951, 999, 2173, 2348, 2408
BseYI CCCAGC 2 cut(s) 420, 2320
BsgI GTGCAG 5 cut(s) 895, 1428, 1749, 1914, 2529
Bsh1236I CGCG 3 cut(s) 278, 1179, 1755
BshFI GGCC 4 cut(s) 170, 383, 1203, 2420
BshNI GGYRCC 1 cut(s) 2209
BsiHKAI GWGCWC 5 cut(s) 236, 674, 798, 1665, 1707
BsiHKCI CYCGRG 1 cut(s) 1664
BslFI GGGAC 1 cut(s) 2206
BslI CCNNNNNNNGG 8 cut(s) 64, 144, 737, 805, 930, 1571, 2155, 2326
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 2206
BsnI GGCC 4 cut(s) 170, 383, 1203, 2420
BsoBI CYCGRG 1 cut(s) 1664
Bsp119I TTCGAA 1 cut(s) 352
Bsp1286I GDGCHC 6 cut(s) 236, 427, 674, 798, 1665, 1707
Bsp1407I TGTACA 1 cut(s) 1088
Bsp1720I GCTNAGC 1 cut(s) 426
Bsp19I CCATGG 2 cut(s) 1530, 1694
BspANI GGCC 4 cut(s) 170, 383, 1203, 2420
BspCNI CTCAG 5 cut(s) 30, 427, 439, 1313, 1440
BspFNI CGCG 3 cut(s) 278, 1179, 1755
BspLI GGNNCC 5 cut(s) 136, 176, 519, 1641, 2211
BspMAI CTGCAG 2 cut(s) 456, 1345
BspMI ACCTGC 2 cut(s) 1721, 2317
BspPI GGATC 8 cut(s) 15, 497, 512, 525, 722, 773, 973, 1535
BspQI GCTCTTC 2 cut(s) 410, 474
BspT104I TTCGAA 1 cut(s) 352
BspT107I GGYRCC 1 cut(s) 2209
BsrBI CCGCTC 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 1088
BsrI ACTGG 7 cut(s) 391, 521, 783, 1253, 1287, 1489, 1641
BssECI CCNNGG 7 cut(s) 276, 1460, 1530, 1589, 1694, 2303, 2429
BssNAI GTATAC 1 cut(s) 698
BssNI GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
BssT1I CCWWGG 3 cut(s) 1530, 1589, 1694
Bst1107I GTATAC 1 cut(s) 698
Bst2UI CCWGG 4 cut(s) 544, 732, 1167, 2431
Bst4CI ACNGT 6 cut(s) 667, 1293, 1307, 1747, 2122, 2261
Bst6I CTCTTC 4 cut(s) 410, 474, 1388, 1509
BstACI GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
BstAPI GCANNNNNTGC 1 cut(s) 1349
BstAUI TGTACA 1 cut(s) 1088
BstBAI YACGTR 2 cut(s) 307, 2375
BstBI TTCGAA 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 459, 1181, 1862
BstDEI CTNAG 6 cut(s) 38, 426, 435, 961, 1300, 1427
BstDSI CCRYGG 4 cut(s) 276, 1530, 1694, 2303
BstFNI CGCG 3 cut(s) 278, 1179, 1755
BstH2I RGCGCY 1 cut(s) 1861
BstHHI GCGC 6 cut(s) 497, 1179, 1236, 1757, 1860, 2326
BstMAI GTCTC 1 cut(s) 71
BstMWI GCNNNNNNNGC 8 cut(s) 219, 380, 494, 1176, 1349, 1985, 2417, 2492
BstNI CCWGG 4 cut(s) 544, 732, 1167, 2431
BstNSI RCATGY 2 cut(s) 495, 1451
BstPAI GACNNNNGTC 1 cut(s) 2139
BstSFI CTRYAG 4 cut(s) 452, 969, 1341, 1569
BstSLI GKGCMC 1 cut(s) 674
BstUI CGCG 3 cut(s) 278, 1179, 1755
BstV1I GCAGC 9 cut(s) 228, 231, 438, 466, 951, 999, 2173, 2348, 2408
BstX2I RGATCY 3 cut(s) 20, 517, 965
BstYI RGATCY 3 cut(s) 20, 517, 965
BstZ17I GTATAC 1 cut(s) 698
BsuI GTATCC 1 cut(s) 1591
BsuRI GGCC 4 cut(s) 170, 383, 1203, 2420
BtgI CCRYGG 4 cut(s) 276, 1530, 1694, 2303
BtgZI GCGATG 2 cut(s) 957, 1736
BtsIMutI CAGTG 5 cut(s) 289, 384, 528, 672, 2127
BveI ACCTGC 2 cut(s) 1721, 2317
Cac8I GCNNGC 3 cut(s) 459, 1181, 1862
CfoI GCGC 6 cut(s) 497, 1179, 1236, 1757, 1860, 2326
Cfr13I GGNCC 4 cut(s) 168, 1202, 1586, 2418
Cfr42I CCGCGG 1 cut(s) 279
CseI GACGC 5 cut(s) 528, 1109, 1991, 2064, 2479
Csp6I GTAC 6 cut(s) 181, 250, 304, 825, 1089, 1398
CspCI CAANNNNNGTGG 6 cut(s) 313, 348, 2285, 2320, 2361, 2396
CviAII CATG 9 cut(s) 492, 641, 883, 1448, 1531, 1695, 1766, 1811, 2458
CviQI GTAC 6 cut(s) 181, 250, 304, 825, 1089, 1398
DdeI CTNAG 6 cut(s) 38, 426, 435, 961, 1300, 1427
DraI TTTAAA 4 cut(s) 1825, 2085, 2316, 2350
EaeI YGGCCR 1 cut(s) 381
Eam1104I CTCTTC 4 cut(s) 410, 474, 1388, 1509
EarI CTCTTC 4 cut(s) 410, 474, 1388, 1509
Ecl136II GAGCTC 4 cut(s) 234, 796, 1663, 1705
Eco130I CCWWGG 3 cut(s) 1530, 1589, 1694
Eco24I GRGCYC 5 cut(s) 236, 427, 798, 1665, 1707
Eco47I GGWCC 1 cut(s) 1586
Eco53kI GAGCTC 4 cut(s) 234, 796, 1663, 1705
Eco57I CTGAAG 1 cut(s) 393
Eco72I CACGTG 1 cut(s) 2375
Eco88I CYCGRG 1 cut(s) 1664
EcoICRI GAGCTC 4 cut(s) 234, 796, 1663, 1705
EcoRII CCWGG 4 cut(s) 542, 730, 1165, 2429
EcoT14I CCWWGG 3 cut(s) 1530, 1589, 1694
EcoT38I GRGCYC 5 cut(s) 236, 427, 798, 1665, 1707
ErhI CCWWGG 3 cut(s) 1530, 1589, 1694
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 9 cut(s) 495, 644, 886, 1451, 1534, 1698, 1769, 1814, 2461
FaqI GGGAC 1 cut(s) 2206
FatI CATG 9 cut(s) 491, 640, 882, 1447, 1530, 1694, 1765, 1810, 2457
FauNDI CATATG 2 cut(s) 926, 2020
FbaI TGATCA 1 cut(s) 2478
FblI GTMKAC 2 cut(s) 697, 1009
FriOI GRGCYC 5 cut(s) 236, 427, 798, 1665, 1707
FspBI CTAG 4 cut(s) 1037, 1644, 2451, 2502
GlaI GCGC 6 cut(s) 496, 1178, 1235, 1756, 1859, 2325
GsaI CCCAGC 2 cut(s) 424, 2324
HaeII RGCGCY 1 cut(s) 1861
HaeIII GGCC 4 cut(s) 170, 383, 1203, 2420
HgaI GACGC 5 cut(s) 528, 1109, 1991, 2064, 2479
HhaI GCGC 6 cut(s) 497, 1179, 1236, 1757, 1860, 2326
Hin1I GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
Hin1II CATG 9 cut(s) 495, 644, 886, 1451, 1534, 1698, 1769, 1814, 2461
Hin6I GCGC 6 cut(s) 495, 1177, 1234, 1755, 1858, 2324
HinP1I GCGC 6 cut(s) 495, 1177, 1234, 1755, 1858, 2324
HindIII AAGCTT 1 cut(s) 866
HphI GGTGA 5 cut(s) 356, 598, 1218, 1445, 1484
Hpy166II GTNNAC 5 cut(s) 672, 698, 1010, 1649, 2107
Hpy188I TCNGA 7 cut(s) 39, 261, 484, 951, 1303, 1774, 2251
Hpy188III TCNNGA 4 cut(s) 50, 1941, 2078, 2198
Hpy8I GTNNAC 5 cut(s) 672, 698, 1010, 1649, 2107
Hpy99I CGWCG 5 cut(s) 56, 296, 774, 2007, 2495
HpyAV CCTTC 4 cut(s) 2119, 2149, 2176, 2451
HpyCH4III ACNGT 6 cut(s) 667, 1293, 1307, 1747, 2122, 2261
HpyCH4IV ACGT 4 cut(s) 306, 548, 772, 2374
HpyF10VI GCNNNNNNNGC 8 cut(s) 219, 380, 494, 1176, 1349, 1985, 2417, 2492
HpyF3I CTNAG 6 cut(s) 38, 426, 435, 961, 1300, 1427
HpySE526I ACGT 4 cut(s) 306, 548, 772, 2374
Hsp92I GRCGYC 5 cut(s) 539, 548, 1101, 2002, 2471
Hsp92II CATG 9 cut(s) 495, 644, 886, 1451, 1534, 1698, 1769, 1814, 2461
HspAI GCGC 6 cut(s) 495, 1177, 1234, 1755, 1858, 2324
Ksp22I TGATCA 1 cut(s) 2478
KspI CCGCGG 1 cut(s) 279
LguI GCTCTTC 2 cut(s) 410, 474
LmnI GCTCC 6 cut(s) 134, 371, 793, 801, 1342, 2491
Lsp1109I GCAGC 9 cut(s) 228, 231, 438, 466, 951, 999, 2173, 2348, 2408
LweI GCATC 2 cut(s) 553, 583
MaeI CTAG 4 cut(s) 1037, 1644, 2451, 2502
MaeII ACGT 4 cut(s) 306, 548, 772, 2374
MaeIII GTNAC 6 cut(s) 376, 1278, 1433, 1843, 1897, 1924
MbiI CCGCTC 1 cut(s) 213
MfeI CAATTG 2 cut(s) 705, 1047
MflI RGATCY 3 cut(s) 20, 517, 965
MhlI GDGCHC 6 cut(s) 236, 427, 674, 798, 1665, 1707
MlyI GAGTC 2 cut(s) 1604, 2188
MmeI TCCRAC 3 cut(s) 48, 284, 1110
MseI TTAA 9 cut(s) 716, 761, 812, 1317, 1497, 1824, 2084, 2315, 2349
MslI CAYNNNNRTG 2 cut(s) 752, 1581
MspA1I CMGCKG 6 cut(s) 278, 451, 457, 1431, 2186, 2339
MunI CAATTG 2 cut(s) 705, 1047
MvaI CCWGG 4 cut(s) 544, 732, 1167, 2431
MvnI CGCG 3 cut(s) 278, 1179, 1755
MwoI GCNNNNNNNGC 8 cut(s) 219, 380, 494, 1176, 1349, 1985, 2417, 2492
NciI CCSGG 6 cut(s) 12, 146, 172, 193, 1462, 2191
NcoI CCATGG 2 cut(s) 1530, 1694
NdeI CATATG 2 cut(s) 926, 2020
NlaIII CATG 9 cut(s) 495, 644, 886, 1451, 1534, 1698, 1769, 1814, 2461
NlaIV GGNNCC 5 cut(s) 136, 176, 519, 1641, 2211
NmeAIII GCCGAG 1 cut(s) 253
NmuCI GTSAC 2 cut(s) 1433, 1924
NspI RCATGY 2 cut(s) 495, 1451
NspV TTCGAA 1 cut(s) 352
PciSI GCTCTTC 2 cut(s) 410, 474
PcsI WCGNNNNNNNCGW 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 542
PleI GAGTC 2 cut(s) 1604, 2188
PmaCI CACGTG 1 cut(s) 2375
PmlI CACGTG 1 cut(s) 2375
PpsI GAGTC 2 cut(s) 1604, 2188
Ppu21I YACGTR 2 cut(s) 307, 2375
PshAI GACNNNNGTC 1 cut(s) 2139
PsiI TTATAA 1 cut(s) 1712
Psp124BI GAGCTC 4 cut(s) 236, 798, 1665, 1707
Psp6I CCWGG 4 cut(s) 542, 730, 1165, 2429
PspCI CACGTG 1 cut(s) 2375
PspFI CCCAGC 2 cut(s) 420, 2320
PspGI CCWGG 4 cut(s) 542, 730, 1165, 2429
PspN4I GGNNCC 5 cut(s) 136, 176, 519, 1641, 2211
PspPI GGNCC 4 cut(s) 168, 1202, 1586, 2418
PstI CTGCAG 2 cut(s) 456, 1345
PsuI RGATCY 3 cut(s) 20, 517, 965
PvuII CAGCTG 4 cut(s) 451, 457, 2186, 2339
RsaI GTAC 6 cut(s) 182, 251, 305, 826, 1090, 1399
RsaNI GTAC 6 cut(s) 181, 250, 304, 825, 1089, 1398
RseI CAYNNNNRTG 2 cut(s) 752, 1581
SacI GAGCTC 4 cut(s) 236, 798, 1665, 1707
SacII CCGCGG 1 cut(s) 279
SapI GCTCTTC 2 cut(s) 410, 474
SaqAI TTAA 9 cut(s) 716, 761, 812, 1317, 1497, 1824, 2084, 2315, 2349
Sau96I GGNCC 4 cut(s) 168, 1202, 1586, 2418
SbfI CCTGCAGG 1 cut(s) 1345
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 2 cut(s) 1604, 2188
SdaI CCTGCAGG 1 cut(s) 1345
SduI GDGCHC 6 cut(s) 236, 427, 674, 798, 1665, 1707
SfaNI GCATC 2 cut(s) 553, 583
SfcI CTRYAG 4 cut(s) 452, 969, 1341, 1569
Sfr303I CCGCGG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 352
SgrBI CCGCGG 1 cut(s) 279
SinI GGWCC 1 cut(s) 1586
SmiMI CAYNNNNRTG 2 cut(s) 752, 1581
SmlI CTYRAG 3 cut(s) 1612, 2008, 2363
SmoI CTYRAG 3 cut(s) 1612, 2008, 2363
Sse8387I CCTGCAGG 1 cut(s) 1345
SspI AATATT 5 cut(s) 1085, 1513, 1876, 1934, 2274
SspMI CTAG 4 cut(s) 1037, 1644, 2451, 2502
SstI GAGCTC 4 cut(s) 236, 798, 1665, 1707
StyI CCWWGG 3 cut(s) 1530, 1589, 1694
TaaI ACNGT 6 cut(s) 667, 1293, 1307, 1747, 2122, 2261
TaiI ACGT 4 cut(s) 309, 551, 775, 2377
TaqI TCGA 9 cut(s) 120, 209, 236, 291, 352, 769, 1063, 1149, 1671
TatI WGTACW 4 cut(s) 249, 824, 1088, 1397
TauI GCSGC 6 cut(s) 65, 68, 216, 278, 396, 399
Tru1I TTAA 9 cut(s) 716, 761, 812, 1317, 1497, 1824, 2084, 2315, 2349
Tru9I TTAA 9 cut(s) 716, 761, 812, 1317, 1497, 1824, 2084, 2315, 2349
TscAI CASTG 5 cut(s) 289, 391, 528, 679, 2127
TseFI GTSAC 2 cut(s) 1433, 1924
TseI GCWGC 9 cut(s) 216, 219, 451, 454, 939, 987, 2186, 2336, 2396
Tsp45I GTSAC 2 cut(s) 1433, 1924
TspGWI ACGGA 3 cut(s) 95, 889, 1943
TspRI CASTG 5 cut(s) 289, 391, 528, 679, 2127
VneI GTGCAC 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 1586
XapI RAATTY 5 cut(s) 347, 915, 1382, 1455, 1829
XceI RCATGY 2 cut(s) 495, 1451
XmiI GTMKAC 2 cut(s) 697, 1009
XspI CTAG 4 cut(s) 1037, 1644, 2451, 2502
ZraI GACGTC 1 cut(s) 549
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.