RchiOBHm_Chr3g0472101

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0007466
Physical Location & Seq
Forward (+)
17921174 .. 17929579
8406 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43783

Sequence Viewer

Length: 2544 bp
ATGGAAATGCCCGGTAGGAGATCGAACTACACGCTTCTCAGCCAAGTCCCCGACGATCACTTCGCTGCGGCGACGGCGACGTCGTTTTACGAGTCGGAGGGGAAGAACAACAATAATAAGGCGAAGGGGGATAGCAGAGGGTTTGATTGGGAAACGGGAGGCGGCGAGTACCGGGCGGCGCCGGCGAATCGGATCGGAAACGTGTACTCGTCGGTCGGATTGCAGCGGCAGTCGAGCGGGAGCAGCTTCGGAGAGAGCTCGCTGTCCGGCGAGTACTACGCGCCGACTCTGTCGACCACGGCGGCGAATGAGATTGACGGATTCGGGTACGTCAACGACGACGGGTTTAAAACCGGCGGAGGAGGCGGTGAGTTCAGAGGGAAAGGAGGAGGGATGGATGGGGGTGTGGGCCCCCCCGGAGGTTCGTCGTCGGGGAAGAGCTGGGCGCAGCAGACTGAGGAGAGTTATCAGCTGCAGTTGGCTTTGGCGCTTCGGCTCTCGTCGGAAGCCACTTGCGCCGATGATCCTAATTTCTTGGATCCAGTGCCGGATGAATCTTCGTCGAGGTTGTCCAGCTCTGCCGATGCAGTTTCGCATCGATTTTGGGTGAATGGCTGTCTGTCATACTTTGACAAAGTGCCTGATGGGTTTTACCTTATTCATGGGATAGATTCGTATGTATGGTCTATGTGCACTGATGTGCAAGAGAGTGGTCGTATACCATCAATTGAATCACTAAAATCTGTTGATCCTGGTACTGGATCTTCCATAGAAGTGGTCTTAATCGATCGACGTAGTGATCCCAGCTTAAAGGAGCTTCAAAATAGGGTCCTCAGCATTTCTTATGCTTGCATAACCACGACAGAGATTGTTGATCAGCTGGCAAAGCTTGTATGCAGCCGTATGGGGGGTTCAGCATCTGTAGGAGAGGCTGAATTTTTCTCCATATGGAGGGAGAGCAGTGATGATCTAAAAGATTGCTTAGGATCTGTAGTTGTTCCAATCGGCAGCCTTTCTATTGGCCTCTGTAGACATCGTGCTTTATTATTCAAGGTTCTAGCTGACACAATTGACCTACCGTGCCGAATTGCCAAAGGCTGTAAATATTGTACAAGAGATGATGCTTCCTCTTGTCTTGTTCGTTTTGGTATTGACAGGGAATTGTTAGTTGATTTGATTGGGAATCCAGGTTGCTTATGTGAGCCTGATTCCTTGCTCAATGGTCCATCATCCATCTCAATTTCTTCACCGCTGCGATTTCCAAGACTCAGAACAGTTGAACCTACCATTGATTTCAGGTCACTGGCCAAACAGTACTTCTCGGACTGTCAGTTGCTTAATCTTGTATTCGATGAAGCTCCGGCAGGAAGTGCTGGTGATGAGGACAATAAAGGATTCTCTATGTATCCTAAGCAGAAGTTTACAGATGGAAACAACCTCTTTCTGGTTTCAGGTCTTGGTGATGACACTTCTATGCATGTTGACGATCGGAATCCCCAGTTCCTTAAATCATTTAATCCTTCTCAGAACATTGTACACCAACAAACAGTATTAAAAGATCAAATCCCCCTAAAGCGTATACCACCTATAGGACATAGAGATATTTCAAGGTTGGATACAAGTAAGGACTCGAGGTTTGGTGAGGGACTTCAAGTGGTTCCTAGTAAACCAAATAAAGAGCTGACTCTCGATGTGGATGATTTGGACATTCCATGGAGTGACCTTGTACTGAAGGAGAGAATCGGTGCAGGTTCTTTTGGAACTGTTCATCGTGCTGATTGGCATGGCTCTGATGTTGCTGTGAAGATTCTTATGGAGCAAGAATTTCATGCTGAACGCTTTAATGAGTTCTTGAGGGAGGTTGCAATTATGAAACGCCTGCGGCATCCAAATATTGTGCTGTTTATGGGTGCAGTTACAAAGCCCCCAAACTTGTCCATAGTCACAGAATATCTATCAAGAGGGAGCTTGTATAGGCTGTTGCATAAACCTGGCCCAATACTGGATGAGAGGCGCCGGTTGTATATGGCTCATGATGTGGCAAAGGGGATGAATTATCTTCATAGGCGGAATCCTCCCATTGTTCATCGAGATTTAAAATCACCAAACCTTTTGGTTGACAAAAAGTATACTGTGAAGGTTTGTGATTTTGGTCTTTCCCGATTAAAGGCCAACACCTTTCTTTCATCAAAATCAGCTGCAGGGACTCCTGAATGGATGGCACCAGAAGTCCTGCGTGATGAACCATCAAATGAGAAGTCAGATGTTTACAGTTTTGGTGTAATATTGTGGGAACTTGCAACATTGCAACAGCCCTGGGGTAATTTGAATCCAGCGCAGGTTGTGGCAGCTGTTGGTTTTAAGAACAAAAGGCTTGAGATTCCACGTGATTTGAATCCTCAAGTGGCTTCTATAATTGAGGCTTGCTGGGCCAATGAGCCCTGGAAACGACCTTCATTTGCTAGTATCATGGAATCTTTGAGGCCATTGATTAAAGCTCCCACACCTCAACCTAGTCATGCAGACATGCCGATACTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

847

Amino Acids

93.13

Weight (kDa)

5.71

Isoelectric Point (pI)

47.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 194 - 400 8.7e-82 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 576 - 827 7e-70 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 577 - 825 8.7e-55 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 79
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 2 cut(s) 1739, 2329
AccB1I GGYRCC 3 cut(s) 178, 2013, 2221
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
AccII CGCG 1 cut(s) 281
AclWI GGATC 8 cut(s) 200, 518, 533, 546, 743, 769, 794, 994
AcoI YGGCCR 1 cut(s) 1305
AcsI RAATTY 2 cut(s) 935, 1823
AcuI CTGAAG 1 cut(s) 1751
AcvI CACGTG 1 cut(s) 2387
AcyI GRCGYC 3 cut(s) 80, 179, 2014
AfaI GTAC 9 cut(s) 170, 206, 275, 329, 757, 1111, 1316, 1536, 1728
AfiI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 8 cut(s) 731, 821, 1051, 1280, 1608, 1652, 2329, 2395
AjnI CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2441
AjuI GAANNNNNNNTTGG 2 cut(s) 1301, 1333
AleI CACNNNNGTG 1 cut(s) 698
Alw21I GWGCWC 2 cut(s) 260, 695
Alw44I GTGCAC 1 cut(s) 691
AlwI GGATC 8 cut(s) 200, 518, 533, 546, 743, 769, 794, 994
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 1630
AoxI GGCC 7 cut(s) 409, 1021, 1305, 1993, 2169, 2430, 2483
ApaI GGGCCC 1 cut(s) 413
ApaLI GTGCAC 1 cut(s) 691
ApoI RAATTY 2 cut(s) 935, 1823
ArsI GACNNNNNNTTYG 6 cut(s) 44, 76, 1619, 1651, 2243, 2275
AspLEI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
AspS9I GGNCC 6 cut(s) 409, 410, 829, 1223, 1994, 2430
AsuC2I CCSGG 3 cut(s) 12, 173, 417
AsuHPI GGTGA 8 cut(s) 380, 619, 1239, 1388, 1472, 1652, 2094, 2530
AvaI CYCGRG 1 cut(s) 1630
AvaII GGWCC 2 cut(s) 829, 1223
BaeGI GKGCMC 2 cut(s) 413, 695
BalI TGGCCA 1 cut(s) 1307
BamHI GGATCC 1 cut(s) 538
BanI GGYRCC 3 cut(s) 178, 2013, 2221
BanII GRGCYC 3 cut(s) 260, 413, 2442
BarI GAAGNNNNNNTAC 2 cut(s) 748, 780
BbrPI CACGTG 1 cut(s) 2387
Bbv12I GWGCWC 2 cut(s) 260, 695
BbvCI CCTCAGC 1 cut(s) 833
BccI CCATC 9 cut(s) 388, 392, 638, 730, 1234, 1241, 1421, 2212, 2254
BceAI ACGGC 3 cut(s) 90, 315, 885
BciT130I CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2443
BciVI GTATCC 2 cut(s) 1416, 1609
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 3 cut(s) 12, 173, 417
BfaI CTAG 4 cut(s) 1058, 1662, 2463, 2514
BfmI CTRYAG 6 cut(s) 473, 921, 990, 1027, 1587, 2199
BfoI RGCGCY 3 cut(s) 182, 491, 2017
BfuAI ACCTGC 2 cut(s) 1739, 2329
BfuI GTATCC 2 cut(s) 1416, 1609
BmcAI AGTACT 2 cut(s) 275, 1316
Bme1390I CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2443
Bme18I GGWCC 2 cut(s) 829, 1223
BmeT110I CYCGRG 1 cut(s) 1630
BmgT120I GGNCC 6 cut(s) 409, 410, 829, 1223, 1994, 2430
BmiI GGNNCC 8 cut(s) 180, 411, 412, 540, 830, 1659, 2015, 2223
BmrFI CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2443
BmrI ACTGGG 1 cut(s) 1492
BmsI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
BmuI ACTGGG 1 cut(s) 1492
Bpu10I CCTNAGC 3 cut(s) 833, 982, 1410
BpuEI CTTGAG 3 cut(s) 1873, 2385, 2396
BpuMI CCSGG 3 cut(s) 12, 173, 417
Bsa29I ATCGAT 2 cut(s) 598, 786
BsaAI YACGTR 1 cut(s) 2387
BsaBI GATNNNNATC 2 cut(s) 1491, 2394
BsaHI GRCGYC 3 cut(s) 80, 179, 2014
BsaJI CCNNGG 6 cut(s) 297, 415, 1712, 2315, 2316, 2441
Bsc4I CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
Bse118I RCCGGY 3 cut(s) 181, 353, 2016
Bse1I ACTGG 5 cut(s) 542, 763, 1308, 1498, 2007
Bse3DI GCAATG 1 cut(s) 2303
Bse8I GATNNNNATC 2 cut(s) 1491, 2394
BseBI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2443
BseCI ATCGAT 2 cut(s) 598, 786
BseDI CCNNGG 6 cut(s) 297, 415, 1712, 2315, 2316, 2441
BseGI GGATG 9 cut(s) 399, 403, 556, 1229, 1702, 1885, 2011, 2055, 2223
BseJI GATNNNNATC 2 cut(s) 1491, 2394
BseLI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
BseMI GCAATG 1 cut(s) 2303
BseMII CTCAG 5 cut(s) 52, 447, 847, 1282, 1538
BseNI ACTGG 5 cut(s) 542, 763, 1308, 1498, 2007
BseRI GAGGAG 3 cut(s) 375, 402, 473
BseSI GKGCMC 2 cut(s) 413, 695
BseYI CCCAGC 3 cut(s) 441, 803, 2427
BsgI GTGCAG 2 cut(s) 1767, 1932
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 3 cut(s) 216, 790, 1489
BshFI GGCC 7 cut(s) 411, 1023, 1307, 1995, 2171, 2432, 2485
BshNI GGYRCC 3 cut(s) 178, 2013, 2221
BshVI ATCGAT 2 cut(s) 598, 786
BsiEI CGRYCG 3 cut(s) 216, 790, 1489
BsiHKAI GWGCWC 2 cut(s) 260, 695
BsiHKCI CYCGRG 1 cut(s) 1630
BsiSI CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
BslFI GGGAC 3 cut(s) 32, 1659, 2218
BslI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
BsmFI GGGAC 3 cut(s) 32, 1659, 2218
BsnI GGCC 7 cut(s) 411, 1023, 1307, 1995, 2171, 2432, 2485
BsoBI CYCGRG 1 cut(s) 1630
Bsp120I GGGCCC 1 cut(s) 409
Bsp1286I GDGCHC 4 cut(s) 260, 413, 695, 2442
Bsp1407I TGTACA 2 cut(s) 1109, 1534
Bsp19I CCATGG 1 cut(s) 1712
BspANI GGCC 7 cut(s) 411, 1023, 1307, 1995, 2171, 2432, 2485
BspCNI CTCAG 5 cut(s) 51, 448, 846, 1281, 1537
BspDI ATCGAT 2 cut(s) 598, 786
BspFNI CGCG 1 cut(s) 281
BspHI TCATGA 1 cut(s) 2032
BspLI GGNNCC 8 cut(s) 180, 411, 412, 540, 830, 1659, 2015, 2223
BspMAI CTGCAG 2 cut(s) 477, 2203
BspMI ACCTGC 2 cut(s) 1739, 2329
BspPI GGATC 8 cut(s) 200, 518, 533, 546, 743, 769, 794, 994
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 3 cut(s) 178, 2013, 2221
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 2303
BsrFI RCCGGY 3 cut(s) 181, 353, 2016
BsrGI TGTACA 2 cut(s) 1109, 1534
BsrI ACTGG 5 cut(s) 542, 763, 1308, 1498, 2007
BssAI RCCGGY 3 cut(s) 181, 353, 2016
BssECI CCNNGG 6 cut(s) 297, 415, 1712, 2315, 2316, 2441
BssNAI GTATAC 3 cut(s) 719, 1580, 2130
BssNI GRCGYC 3 cut(s) 80, 179, 2014
BssT1I CCWWGG 1 cut(s) 1712
Bst1107I GTATAC 3 cut(s) 719, 1580, 2130
Bst2UI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2443
Bst4CI ACNGT 8 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273
Bst6I CTCTTC 1 cut(s) 431
BstACI GRCGYC 3 cut(s) 80, 179, 2014
BstAPI GCANNNNNTGC 1 cut(s) 1370
BstAUI TGTACA 2 cut(s) 1109, 1534
BstBAI YACGTR 1 cut(s) 2387
BstC8I GCNNGC 6 cut(s) 183, 260, 850, 882, 1880, 2425
BstDEI CTNAG 7 cut(s) 38, 456, 833, 982, 1268, 1410, 1524
BstDSI CCRYGG 2 cut(s) 297, 1712
BstF5I GGATG 9 cut(s) 399, 403, 556, 1229, 1702, 1885, 2011, 2055, 2223
BstFNI CGCG 1 cut(s) 281
BstH2I RGCGCY 3 cut(s) 182, 491, 2017
BstHHI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
BstMCI CGRYCG 3 cut(s) 216, 790, 1489
BstMWI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 2429
BstNI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2443
BstNSI RCATGY 2 cut(s) 1481, 2530
BstSCI CCNGG 8 cut(s) 10, 171, 415, 751, 1186, 1990, 2315, 2441
BstSFI CTRYAG 6 cut(s) 473, 921, 990, 1027, 1587, 2199
BstSLI GKGCMC 2 cut(s) 413, 695
BstUI CGCG 1 cut(s) 281
BstX2I RGATCY 3 cut(s) 538, 761, 986
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 3 cut(s) 538, 761, 986
BstZ17I GTATAC 3 cut(s) 719, 1580, 2130
Bsu15I ATCGAT 2 cut(s) 598, 786
BsuI GTATCC 2 cut(s) 1416, 1609
BsuRI GGCC 7 cut(s) 411, 1023, 1307, 1995, 2171, 2432, 2485
BsuTUI ATCGAT 2 cut(s) 598, 786
BtgI CCRYGG 2 cut(s) 297, 1712
BtsCI GGATG 9 cut(s) 399, 403, 556, 1229, 1702, 1885, 2011, 2055, 2223
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 4 cut(s) 549, 693, 967, 1301
BveI ACCTGC 2 cut(s) 1739, 2329
Cac8I GCNNGC 6 cut(s) 183, 260, 850, 882, 1880, 2425
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 2032
CfoI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
Cfr10I RCCGGY 3 cut(s) 181, 353, 2016
Cfr13I GGNCC 6 cut(s) 409, 410, 829, 1223, 1994, 2430
ClaI ATCGAT 2 cut(s) 598, 786
Csp6I GTAC 9 cut(s) 169, 205, 274, 328, 756, 1110, 1315, 1535, 1727
CspCI CAANNNNNGTGG 2 cut(s) 2373, 2408
CviAII CATG 9 cut(s) 662, 1478, 1713, 1784, 1829, 2033, 2470, 2519, 2527
CviQI GTAC 9 cut(s) 169, 205, 274, 328, 756, 1110, 1315, 1535, 1727
DdeI CTNAG 7 cut(s) 38, 456, 833, 982, 1268, 1410, 1524
DinI GGCGCC 2 cut(s) 180, 2015
DraI TTTAAA 2 cut(s) 349, 2097
DrdI GACNNNNNNGTC 1 cut(s) 79
DseDI GACNNNNNNGTC 1 cut(s) 79
EaeI YGGCCR 1 cut(s) 1305
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
EciI GGCGGA 2 cut(s) 372, 2083
Ecl136II GAGCTC 1 cut(s) 258
Eco130I CCWWGG 1 cut(s) 1712
Eco24I GRGCYC 3 cut(s) 260, 413, 2442
Eco47I GGWCC 2 cut(s) 829, 1223
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 1751
Eco72I CACGTG 1 cut(s) 2387
Eco88I CYCGRG 1 cut(s) 1630
EcoICRI GAGCTC 1 cut(s) 258
EcoO109I RGGNCCY 2 cut(s) 410, 829
EcoRII CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2441
EcoT14I CCWWGG 1 cut(s) 1712
EcoT22I ATGCAT 1 cut(s) 1479
EcoT38I GRGCYC 3 cut(s) 260, 413, 2442
EgeI GGCGCC 2 cut(s) 180, 2015
EheI GGCGCC 2 cut(s) 180, 2015
ErhI CCWWGG 1 cut(s) 1712
FaeI CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2473, 2522, 2530
FaqI GGGAC 3 cut(s) 32, 1659, 2218
FatI CATG 9 cut(s) 661, 1477, 1712, 1783, 1828, 2032, 2469, 2518, 2526
FauI CCCGC 1 cut(s) 230
FauNDI CATATG 1 cut(s) 947
FbaI TGATCA 1 cut(s) 874
FblI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
FokI GGATG 9 cut(s) 406, 410, 563, 1216, 1709, 1872, 2018, 2062, 2230
FriOI GRGCYC 3 cut(s) 260, 413, 2442
FspBI CTAG 4 cut(s) 1058, 1662, 2463, 2514
GlaI GCGC 7 cut(s) 180, 282, 447, 489, 517, 2015, 2337
GsaI CCCAGC 3 cut(s) 445, 807, 2431
HaeII RGCGCY 3 cut(s) 182, 491, 2017
HaeIII GGCC 7 cut(s) 411, 1023, 1307, 1995, 2171, 2432, 2485
HapII CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
HhaI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
Hin1I GRCGYC 3 cut(s) 80, 179, 2014
Hin1II CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2473, 2522, 2530
Hin6I GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
HinP1I GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
HincII GTYRAC 4 cut(s) 294, 334, 1483, 2119
HindII GTYRAC 4 cut(s) 294, 334, 1483, 2119
HindIII AAGCTT 1 cut(s) 887
HpaII CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
HphI GGTGA 8 cut(s) 380, 619, 1239, 1388, 1472, 1652, 2094, 2530
Hpy188III TCNNGA 7 cut(s) 1688, 1852, 1959, 2033, 2090, 2160, 2210
HpyAV CCTTC 5 cut(s) 118, 1530, 1726, 2131, 2463
HpyCH4III ACNGT 8 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273
HpyCH4IV ACGT 5 cut(s) 80, 201, 330, 793, 2386
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 2429
HpyF3I CTNAG 7 cut(s) 38, 456, 833, 982, 1268, 1410, 1524
HpySE526I ACGT 5 cut(s) 80, 201, 330, 793, 2386
Hsp92I GRCGYC 3 cut(s) 80, 179, 2014
Hsp92II CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2473, 2522, 2530
HspAI GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
KasI GGCGCC 2 cut(s) 178, 2013
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 874
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 6 cut(s) 240, 814, 1363, 1816, 1965, 2503
LweI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
MaeI CTAG 4 cut(s) 1058, 1662, 2463, 2514
MaeII ACGT 5 cut(s) 80, 201, 330, 793, 2386
MaeIII GTNAC 4 cut(s) 1299, 1718, 1915, 1942
MbiI CCGCTC 1 cut(s) 237
MboII GAAGA 7 cut(s) 115, 448, 549, 756, 1236, 1816, 2051
MfeI CAATTG 2 cut(s) 726, 1068
MflI RGATCY 3 cut(s) 538, 761, 986
MhlI GDGCHC 4 cut(s) 260, 413, 695, 2442
MlsI TGGCCA 1 cut(s) 1307
MluNI TGGCCA 1 cut(s) 1307
Mly113I GGCGCC 2 cut(s) 179, 2014
MlyI GAGTC 6 cut(s) 101, 280, 1260, 1622, 1678, 2200
MmeI TCCRAC 4 cut(s) 75, 196, 483, 1593
Mox20I TGGCCA 1 cut(s) 1307
Mph1103I ATGCAT 1 cut(s) 1479
MreI CGCCGGCG 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 181
MscI TGGCCA 1 cut(s) 1307
MslI CAYNNNNRTG 3 cut(s) 698, 773, 1472
Msp20I TGGCCA 1 cut(s) 1307
MspA1I CMGCKG 6 cut(s) 226, 472, 880, 1252, 2198, 2351
MspI CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
MspR9I CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2443
MunI CAATTG 2 cut(s) 726, 1068
MvaI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2443
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 2429
NaeI GCCGGC 1 cut(s) 183
NarI GGCGCC 2 cut(s) 179, 2014
NciI CCSGG 3 cut(s) 12, 173, 417
NcoI CCATGG 1 cut(s) 1712
NdeI CATATG 1 cut(s) 947
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2473, 2522, 2530
NlaIV GGNNCC 8 cut(s) 180, 411, 412, 540, 830, 1659, 2015, 2223
NmuCI GTSAC 3 cut(s) 1299, 1718, 1942
NsiI ATGCAT 1 cut(s) 1479
NspI RCATGY 2 cut(s) 1481, 2530
OliI CACNNNNGTG 1 cut(s) 698
PaeR7I CTCGAG 1 cut(s) 1630
PagI TCATGA 1 cut(s) 2032
PasI CCCWGGG 1 cut(s) 2316
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 336
PdiI GCCGGC 1 cut(s) 183
PflFI GACNNNGTC 1 cut(s) 289
Ple19I CGATCG 2 cut(s) 790, 1489
PleI GAGTC 6 cut(s) 100, 280, 1260, 1622, 1678, 2200
PluTI GGCGCC 2 cut(s) 182, 2017
PmaCI CACGTG 1 cut(s) 2387
PmlI CACGTG 1 cut(s) 2387
PpsI GAGTC 6 cut(s) 100, 280, 1260, 1622, 1678, 2200
Ppu21I YACGTR 1 cut(s) 2387
PpuMI RGGWCCY 1 cut(s) 829
Psp124BI GAGCTC 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2441
PspCI CACGTG 1 cut(s) 2387
PspFI CCCAGC 3 cut(s) 441, 803, 2427
PspGI CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2441
PspN4I GGNNCC 8 cut(s) 180, 411, 412, 540, 830, 1659, 2015, 2223
PspOMI GGGCCC 1 cut(s) 409
PspPI GGNCC 6 cut(s) 409, 410, 829, 1223, 1994, 2430
PspPPI RGGWCCY 1 cut(s) 829
PspXI VCTCGAGB 1 cut(s) 1630
PsrI GAACNNNNNNTAC 2 cut(s) 895, 927
PstI CTGCAG 2 cut(s) 477, 2203
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 3 cut(s) 538, 761, 986
PsyI GACNNNGTC 1 cut(s) 289
PvuI CGATCG 2 cut(s) 790, 1489
PvuII CAGCTG 4 cut(s) 472, 880, 2198, 2351
RsaI GTAC 9 cut(s) 170, 206, 275, 329, 757, 1111, 1316, 1536, 1728
RsaNI GTAC 9 cut(s) 169, 205, 274, 328, 756, 1110, 1315, 1535, 1727
RseI CAYNNNNRTG 3 cut(s) 698, 773, 1472
SacI GAGCTC 1 cut(s) 260
SalI GTCGAC 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 431
Sau96I GGNCC 6 cut(s) 409, 410, 829, 1223, 1994, 2430
ScaI AGTACT 2 cut(s) 275, 1316
SchI GAGTC 6 cut(s) 101, 280, 1260, 1622, 1678, 2200
ScrFI CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2443
SduI GDGCHC 4 cut(s) 260, 413, 695, 2442
SfaNI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
SfcI CTRYAG 6 cut(s) 473, 921, 990, 1027, 1587, 2199
SfoI GGCGCC 2 cut(s) 180, 2015
Sfr274I CTCGAG 1 cut(s) 1630
SgrAI CRCCGGYG 1 cut(s) 181
SinI GGWCC 2 cut(s) 829, 1223
SlaI CTCGAG 1 cut(s) 1630
SmiMI CAYNNNNRTG 3 cut(s) 698, 773, 1472
SmlI CTYRAG 4 cut(s) 1630, 1852, 2375, 2400
SmoI CTYRAG 4 cut(s) 1630, 1852, 2375, 2400
SspDI GGCGCC 2 cut(s) 178, 2013
SspI AATATT 3 cut(s) 1106, 1894, 2286
SspMI CTAG 4 cut(s) 1058, 1662, 2463, 2514
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 8 cut(s) 10, 171, 415, 751, 1186, 1990, 2315, 2441
StyI CCWWGG 1 cut(s) 1712
TaaI ACNGT 8 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273
TaiI ACGT 5 cut(s) 83, 204, 333, 796, 2389
TaqII GACCGA 1 cut(s) 202
TatI WGTACW 6 cut(s) 204, 273, 1109, 1314, 1534, 1726
TauI GCSGC 6 cut(s) 71, 165, 179, 229, 305, 1885
TscAI CASTG 4 cut(s) 549, 700, 967, 1308
TseFI GTSAC 3 cut(s) 1299, 1718, 1942
Tsp45I GTSAC 3 cut(s) 1299, 1718, 1942
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 4 cut(s) 549, 700, 967, 1308
Tth111I GACNNNGTC 1 cut(s) 289
VneI GTGCAC 1 cut(s) 691
VpaK11BI GGWCC 2 cut(s) 829, 1223
XapI RAATTY 2 cut(s) 935, 1823
XceI RCATGY 2 cut(s) 1481, 2530
XhoI CTCGAG 1 cut(s) 1630
XmiI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
XspI CTAG 4 cut(s) 1058, 1662, 2463, 2514
ZraI GACGTC 1 cut(s) 81
ZrmI AGTACT 2 cut(s) 275, 1316
Zsp2I ATGCAT 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.