RLG00000024103

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007466
Physical Location & Seq
Reverse (-)
31916461 .. 31923663
7203 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024103

Sequence Viewer

Length: 2571 bp
ATGGAAATGCCCGGTAGGAGATCGAACTACACGCTTCTCAGCCAAGTCCCCGACGATCACTTCGCGGCGGCTACTGCGACGTCGTTTTACGAGTCGGAGGGGAAGAACAACAATAATAAGGCGAAGGGGGATAGCAGAGGGTTTGATTGGGAAACGGGAGGCGGCGAGTACCGGGCGGCGCCGGCGAATCGGATGGGAAACGTGTACTCGTCGGTCGGATTGCAGCGGCAGTCGAGCGGGAGCAGCTTCGGAGAGAGCTCGCTGTCCGGCGAGTACTACGCGCCGACTCTGTCGACCACGGCGGCGAATGAGATTGACGGATTCGGGTACGTCAACGACGACGGGTTTAAAACCGGCGGAGGAGGCGGTGAGTTCAGAGGGAAAGGAGGAGGGATGGATGGGGCTGTGGGGCCCCCCGGAGGCTCGTCGTCGGGGAAGAGCTGGGCGCAGCAGACTGAGGAGAGTTATCAGCTGCAGTTGGCTTTGGCGCTTCGGCTCTCGTCGGAAGCCACTTGCGCCGATGATCCTAATTTCTTGGATCCAGTGCCGGATGAGTCTTCGTCGAGGTTGTCCAGCTTTGCCGATGCAGTTTCGCATCGATTTTGGGTGAATGGCTGTCTGTCATACTTTGACAAAGTGCCTGATGGGTTTTACCTTATTCATGGGATAGATTCGTATGTATGGTCTATGTGCACTGATGTGCAAGAGAGTGGTCGTATACCATCAATTGAATCACTAAGATCTGTTGATCCTGGTACTGGATCTTCCATAGAAGTGGTCTTAATCGATCGACGTAGTGATCCCAGCTTAAAGGAGCTTCAAAATAGGGTCCTCAGCATTTCTTGTGCTTGCATAACCACGACAGAGATTGTTGATCAGCTGGCAAAGCTTGTATGCAGCCGTATGGGGGGTTCAGCATCTGTGGGAGAGTCTGAATTTTTCTCCATATGGAGGGAGAGCAGTGATGATCTAAAAGATTGCTTAGGATCTGTAGTTGTTCCAATCGGCAGCCTTTCTATTGGTCTCTGTAGACATCGTGCTTTATTATTCAAGGTTCTAGCTGACACAATTGACCTACCGTGCCGAATTGCCAAAGGCTGTAAATATTGTACAAGAGATGATGCTTCCTCTTGTCTTGTTCGTTTTGGTATTGACAGGGAATTGTTAGTTGATTTGATTGGGAATCCAGGTTGCTTATGTGAGCCTGATTCCTTGCTCAATGGTCCATCATCCATCTCAATTTCTTCACCGCTGCGGTTTCCAAGACTCAGAACAGTTGAACCTACCATTGATTTCAGGTCACTGGCCAAACAGTACTTCTCGGACTGTCAGTTGCTTAACCTTGTATTCAATGAAGCTCCGGCAGGAAGTGCTGGTGATGAGGACAATAAAGGATTCTCTATGTATCCTAAGCAGAAGTTTACAGATGGAAGCAACCTCTTTCTGGTTTCAGGTCTTGGTGATGACACTTCTATGCATGTTGACGATCGGAATCCCCAGTTCCTTAAATCATTTAATCCTTCTCAAAACATTGTACACCAACAAACAGTATTAAAAGATCAAATCCCCCTAAAGCGTATACCGCCTATAGGACATAGAGATATTTCAAGGTTGGATACAAGTAAGGACTCGAGGTTTGGTGAGGGACATCAAGTGGTTCCTAGTAAACCAAATAAAGAGCCGACTCTCGATGTGGATGATTTGGACATTCCATGGAGTGACCTTGTACTGAAGGAGAGAATCGGTGCAGGTTCTTTTGGAACTGTTCATCGTGCTGATTGGCATGGCTCTGATGTTGCTGTGAAGATTCTTATGGAGCAAGAATTTCATGCCGAACGCTTTAAAGAGTTCTTGAGGGAGGTTGCAATTATGAAACGCCTGCGGCATCCAAATATTGTGCTGTTTATGGGTGCAGTTACAAAGCCCCCAAACTTGTCCATAGTCACAGAATATCTATCAAGAGGGAGCTTGTATAGGCTGTTGCATAAACCTGGCCCAATGCTGGATGAGAGGCGCCGGTTGAATATGGCTCATGATGTGGCAAAGGGGATGAATTATCTTCATAGGCGGAATCCTCCCATTGTTCATCGAGATTTAAAATCGCCAAACCTTTTGGTTGACAAAAAGTATACTGTGAAGGTTTGTGATTTTGGTCTTTCCCGATTAAAGGCCAACACCTTTCTTTCATCAAAATCAGCTGCAGGGACTCCTGAATGGATGGCACCAGAAGTCCTGCGTGATGAACCATCAAATGAGAAGTCAGATGTTTACAGTTTTGGTGTAATATTGTGGGAACTTGCGACATTGCAACAGCCCTGGGGTAATTTGAATCCAGCGCAGAATATTGCAGAATGGTGTTTCTCTGAGCCCTGGAAACGACCTTCATTTGCTAGTATCATGGAATCTTTGAGGCCATTGATTAAAGCTCCCACACCTCAACCTAGATTACATGCTGTTCATAGTCTCGGAGTCATTGTACATTACTGTTCTTATCGCCAATTGAGAATTCTGATTCTTTATGCAAATCAAGGACTAAAGTGCCCATGGTGTCTGCATTGCCATCCCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

857

Amino Acids

94.61

Weight (kDa)

6.19

Isoelectric Point (pI)

46.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 194 - 400 4.3e-82 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 576 - 783 4.3e-58 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 577 - 780 4.8e-51 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 83
Acc36I ACCTGC 1 cut(s) 1739
AccB1I GGYRCC 3 cut(s) 178, 2013, 2221
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
AccII CGCG 2 cut(s) 65, 281
AclWI GGATC 7 cut(s) 518, 533, 546, 743, 769, 794, 994
AcoI YGGCCR 1 cut(s) 1305
AcsI RAATTY 3 cut(s) 935, 1823, 2505
AcuI CTGAAG 1 cut(s) 1751
AcyI GRCGYC 3 cut(s) 80, 179, 2014
AfiI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
AflIII ACRYGT 1 cut(s) 201
AgsI TTSAA 8 cut(s) 731, 821, 1051, 1280, 1351, 1608, 2023, 2329
AjnI CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2369
AjuI GAANNNNNNNTTGG 4 cut(s) 1301, 1333, 2490, 2522
AleI CACNNNNGTG 1 cut(s) 698
Alw21I GWGCWC 2 cut(s) 260, 695
Alw26I GTCTC 1 cut(s) 1028
Alw44I GTGCAC 1 cut(s) 691
AlwI GGATC 7 cut(s) 518, 533, 546, 743, 769, 794, 994
AlwNI CAGNNNCTG 1 cut(s) 920
Ama87I CYCGRG 1 cut(s) 1630
AoxI GGCC 5 cut(s) 410, 1305, 1993, 2169, 2411
ApaI GGGCCC 1 cut(s) 414
ApaLI GTGCAC 1 cut(s) 691
ApeKI GCWGC 8 cut(s) 223, 243, 448, 472, 897, 1008, 1252, 2198
ApoI RAATTY 3 cut(s) 935, 1823, 2505
ArsI GACNNNNNNTTYG 6 cut(s) 44, 76, 1619, 1651, 2243, 2275
AspLEI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
AspS9I GGNCC 5 cut(s) 410, 411, 829, 1223, 1994
AsuC2I CCSGG 3 cut(s) 12, 173, 417
AsuHPI GGTGA 6 cut(s) 380, 619, 1239, 1388, 1472, 1652
AvaI CYCGRG 1 cut(s) 1630
AvaII GGWCC 2 cut(s) 829, 1223
BaeGI GKGCMC 3 cut(s) 414, 695, 2543
BalI TGGCCA 1 cut(s) 1307
BamHI GGATCC 1 cut(s) 538
BanI GGYRCC 3 cut(s) 178, 2013, 2221
BanII GRGCYC 3 cut(s) 260, 414, 2370
BarI GAAGNNNNNNTAC 2 cut(s) 748, 780
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 2 cut(s) 260, 695
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 8 cut(s) 235, 255, 459, 460, 909, 1020, 1239, 2185
BceAI ACGGC 2 cut(s) 315, 885
BciT130I CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2371
BciVI GTATCC 2 cut(s) 1416, 1609
BclI TGATCA 1 cut(s) 874
BcnI CCSGG 3 cut(s) 12, 173, 417
BcoDI GTCTC 2 cut(s) 1028, 2468
BfaI CTAG 4 cut(s) 1058, 1662, 2391, 2442
BfmI CTRYAG 5 cut(s) 473, 990, 1027, 1587, 2199
BfoI RGCGCY 3 cut(s) 182, 491, 2017
BfuAI ACCTGC 1 cut(s) 1739
BfuI GTATCC 2 cut(s) 1416, 1609
BglII AGATCT 1 cut(s) 740
BmcAI AGTACT 2 cut(s) 275, 1316
Bme1390I CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2371
Bme18I GGWCC 2 cut(s) 829, 1223
BmeT110I CYCGRG 1 cut(s) 1630
BmgT120I GGNCC 5 cut(s) 410, 411, 829, 1223, 1994
BmiI GGNNCC 9 cut(s) 180, 411, 412, 413, 540, 830, 1659, 2015, 2223
BmrFI CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2371
BmrI ACTGGG 1 cut(s) 1492
BmsI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
BmuI ACTGGG 1 cut(s) 1492
BpiI GAAGAC 1 cut(s) 549
Bpu10I CCTNAGC 3 cut(s) 833, 982, 1410
BpuEI CTTGAG 1 cut(s) 1873
BpuMI CCSGG 3 cut(s) 12, 173, 417
Bsa29I ATCGAT 2 cut(s) 598, 786
BsaBI GATNNNNATC 1 cut(s) 1491
BsaHI GRCGYC 3 cut(s) 80, 179, 2014
BsaI GGTCTC 1 cut(s) 1028
BsaJI CCNNGG 7 cut(s) 297, 415, 1712, 2315, 2316, 2369, 2543
BsaXI ACNNNNNCTCC 2 cut(s) 2460, 2490
Bsc4I CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
Bse118I RCCGGY 3 cut(s) 181, 353, 2016
Bse1I ACTGG 4 cut(s) 542, 763, 1308, 1498
Bse3DI GCAATG 2 cut(s) 2303, 2554
Bse8I GATNNNNATC 1 cut(s) 1491
BseBI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2371
BseCI ATCGAT 2 cut(s) 598, 786
BseDI CCNNGG 7 cut(s) 297, 415, 1712, 2315, 2316, 2369, 2543
BseJI GATNNNNATC 1 cut(s) 1491
BseLI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
BseMI GCAATG 2 cut(s) 2303, 2554
BseMII CTCAG 5 cut(s) 52, 447, 847, 1282, 2355
BseNI ACTGG 4 cut(s) 542, 763, 1308, 1498
BseRI GAGGAG 3 cut(s) 375, 402, 473
BseSI GKGCMC 3 cut(s) 414, 695, 2543
BseXI GCAGC 8 cut(s) 235, 255, 459, 460, 909, 1020, 1239, 2185
BseYI CCCAGC 2 cut(s) 441, 803
BsgI GTGCAG 2 cut(s) 1767, 1932
Bsh1236I CGCG 2 cut(s) 65, 281
Bsh1285I CGRYCG 3 cut(s) 216, 790, 1489
BshFI GGCC 5 cut(s) 412, 1307, 1995, 2171, 2413
BshNI GGYRCC 3 cut(s) 178, 2013, 2221
BshVI ATCGAT 2 cut(s) 598, 786
BsiEI CGRYCG 3 cut(s) 216, 790, 1489
BsiHKAI GWGCWC 2 cut(s) 260, 695
BsiHKCI CYCGRG 1 cut(s) 1630
BsiSI CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
BslFI GGGAC 3 cut(s) 32, 1659, 2218
BslI CCNNNNNNNGG 8 cut(s) 419, 758, 907, 951, 1444, 1589, 2002, 2167
BsmAI GTCTC 2 cut(s) 1028, 2468
BsmFI GGGAC 3 cut(s) 32, 1659, 2218
BsnI GGCC 5 cut(s) 412, 1307, 1995, 2171, 2413
Bso31I GGTCTC 1 cut(s) 1028
BsoBI CYCGRG 1 cut(s) 1630
Bsp120I GGGCCC 1 cut(s) 410
Bsp1286I GDGCHC 5 cut(s) 260, 414, 695, 2370, 2543
Bsp1407I TGTACA 3 cut(s) 1109, 1534, 2476
Bsp19I CCATGG 2 cut(s) 1712, 2543
BspANI GGCC 5 cut(s) 412, 1307, 1995, 2171, 2413
BspCNI CTCAG 5 cut(s) 51, 448, 846, 1281, 2356
BspDI ATCGAT 2 cut(s) 598, 786
BspFNI CGCG 2 cut(s) 65, 281
BspHI TCATGA 1 cut(s) 2032
BspLI GGNNCC 9 cut(s) 180, 411, 412, 413, 540, 830, 1659, 2015, 2223
BspMAI CTGCAG 2 cut(s) 477, 2203
BspMI ACCTGC 1 cut(s) 1739
BspPI GGATC 7 cut(s) 518, 533, 546, 743, 769, 794, 994
BspQI GCTCTTC 1 cut(s) 431
BspT107I GGYRCC 3 cut(s) 178, 2013, 2221
BspTNI GGTCTC 1 cut(s) 1028
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 2 cut(s) 2303, 2554
BsrFI RCCGGY 3 cut(s) 181, 353, 2016
BsrGI TGTACA 3 cut(s) 1109, 1534, 2476
BsrI ACTGG 4 cut(s) 542, 763, 1308, 1498
BssAI RCCGGY 3 cut(s) 181, 353, 2016
BssECI CCNNGG 7 cut(s) 297, 415, 1712, 2315, 2316, 2369, 2543
BssNAI GTATAC 3 cut(s) 719, 1580, 2130
BssNI GRCGYC 3 cut(s) 80, 179, 2014
BssT1I CCWWGG 2 cut(s) 1712, 2543
Bst1107I GTATAC 3 cut(s) 719, 1580, 2130
Bst2UI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2371
Bst4CI ACNGT 9 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273, 2486
Bst6I CTCTTC 1 cut(s) 431
BstACI GRCGYC 3 cut(s) 80, 179, 2014
BstAPI GCANNNNNTGC 1 cut(s) 1370
BstAUI TGTACA 3 cut(s) 1109, 1534, 2476
BstC8I GCNNGC 5 cut(s) 183, 260, 850, 882, 1880
BstDEI CTNAG 8 cut(s) 38, 456, 737, 833, 982, 1268, 1410, 2364
BstDSI CCRYGG 3 cut(s) 297, 1712, 2543
BstFNI CGCG 2 cut(s) 65, 281
BstH2I RGCGCY 3 cut(s) 182, 491, 2017
BstHHI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
BstMAI GTCTC 2 cut(s) 1028, 2468
BstMCI CGRYCG 3 cut(s) 216, 790, 1489
BstMWI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 1582
BstNI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2371
BstNSI RCATGY 2 cut(s) 1481, 2453
BstSCI CCNGG 8 cut(s) 10, 171, 415, 751, 1186, 1990, 2315, 2369
BstSFI CTRYAG 5 cut(s) 473, 990, 1027, 1587, 2199
BstSLI GKGCMC 3 cut(s) 414, 695, 2543
BstUI CGCG 2 cut(s) 65, 281
BstV1I GCAGC 8 cut(s) 235, 255, 459, 460, 909, 1020, 1239, 2185
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 4 cut(s) 538, 740, 761, 986
BstXI CCANNNNNNTGG 1 cut(s) 775
BstYI RGATCY 4 cut(s) 538, 740, 761, 986
BstZ17I GTATAC 3 cut(s) 719, 1580, 2130
Bsu15I ATCGAT 2 cut(s) 598, 786
BsuI GTATCC 2 cut(s) 1416, 1609
BsuRI GGCC 5 cut(s) 412, 1307, 1995, 2171, 2413
BsuTUI ATCGAT 2 cut(s) 598, 786
BtgI CCRYGG 3 cut(s) 297, 1712, 2543
BtsI GCAGTG 1 cut(s) 967
BtsIMutI CAGTG 4 cut(s) 549, 693, 967, 1301
BveI ACCTGC 1 cut(s) 1739
Cac8I GCNNGC 5 cut(s) 183, 260, 850, 882, 1880
CaiI CAGNNNCTG 1 cut(s) 920
CciI TCATGA 1 cut(s) 2032
CfoI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
Cfr10I RCCGGY 3 cut(s) 181, 353, 2016
Cfr13I GGNCC 5 cut(s) 410, 411, 829, 1223, 1994
ClaI ATCGAT 2 cut(s) 598, 786
CviAII CATG 9 cut(s) 662, 1478, 1713, 1784, 1829, 2033, 2398, 2450, 2544
DdeI CTNAG 8 cut(s) 38, 456, 737, 833, 982, 1268, 1410, 2364
DinI GGCGCC 2 cut(s) 180, 2015
DraI TTTAAA 3 cut(s) 349, 1843, 2097
EaeI YGGCCR 1 cut(s) 1305
Eam1104I CTCTTC 1 cut(s) 431
EarI CTCTTC 1 cut(s) 431
EciI GGCGGA 2 cut(s) 372, 2083
Ecl136II GAGCTC 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 1712, 2543
Eco24I GRGCYC 3 cut(s) 260, 414, 2370
Eco31I GGTCTC 1 cut(s) 1028
Eco47I GGWCC 2 cut(s) 829, 1223
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 1751
Eco88I CYCGRG 1 cut(s) 1630
EcoICRI GAGCTC 1 cut(s) 258
EcoO109I RGGNCCY 3 cut(s) 410, 411, 829
EcoRI GAATTC 1 cut(s) 2505
EcoRII CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2369
EcoT14I CCWWGG 2 cut(s) 1712, 2543
EcoT22I ATGCAT 1 cut(s) 1479
EcoT38I GRGCYC 3 cut(s) 260, 414, 2370
EgeI GGCGCC 2 cut(s) 180, 2015
EheI GGCGCC 2 cut(s) 180, 2015
ErhI CCWWGG 2 cut(s) 1712, 2543
FaeI CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2401, 2453, 2547
FaqI GGGAC 3 cut(s) 32, 1659, 2218
FatI CATG 9 cut(s) 661, 1477, 1712, 1783, 1828, 2032, 2397, 2449, 2543
FauI CCCGC 1 cut(s) 230
FauNDI CATATG 1 cut(s) 947
FbaI TGATCA 1 cut(s) 874
FblI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
FriOI GRGCYC 3 cut(s) 260, 414, 2370
FspBI CTAG 4 cut(s) 1058, 1662, 2391, 2442
GlaI GCGC 7 cut(s) 180, 282, 447, 489, 517, 2015, 2337
GsaI CCCAGC 2 cut(s) 445, 807
HaeII RGCGCY 3 cut(s) 182, 491, 2017
HaeIII GGCC 5 cut(s) 412, 1307, 1995, 2171, 2413
HapII CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
HhaI GCGC 7 cut(s) 181, 283, 448, 490, 518, 2016, 2338
Hin1I GRCGYC 3 cut(s) 80, 179, 2014
Hin1II CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2401, 2453, 2547
Hin6I GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
HinP1I GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
HincII GTYRAC 4 cut(s) 294, 334, 1483, 2119
HindII GTYRAC 4 cut(s) 294, 334, 1483, 2119
HindIII AAGCTT 1 cut(s) 887
HpaII CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
HphI GGTGA 6 cut(s) 380, 619, 1239, 1388, 1472, 1652
Hpy188III TCNNGA 7 cut(s) 1688, 1852, 1959, 2033, 2090, 2160, 2210
HpyAV CCTTC 5 cut(s) 118, 1530, 1726, 2131, 2391
HpyCH4III ACNGT 9 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273, 2486
HpyCH4IV ACGT 4 cut(s) 80, 201, 330, 793
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 1582
HpyF3I CTNAG 8 cut(s) 38, 456, 737, 833, 982, 1268, 1410, 2364
HpySE526I ACGT 4 cut(s) 80, 201, 330, 793
Hsp92I GRCGYC 3 cut(s) 80, 179, 2014
Hsp92II CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2401, 2453, 2547
HspAI GCGC 7 cut(s) 179, 281, 446, 488, 516, 2014, 2336
KasI GGCGCC 2 cut(s) 178, 2013
KroI GCCGGC 1 cut(s) 181
KroNI GCCGGC 1 cut(s) 183
Ksp22I TGATCA 1 cut(s) 874
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 6 cut(s) 240, 814, 1363, 1816, 1965, 2431
Lsp1109I GCAGC 8 cut(s) 235, 255, 459, 460, 909, 1020, 1239, 2185
LweI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
MaeI CTAG 4 cut(s) 1058, 1662, 2391, 2442
MaeII ACGT 4 cut(s) 80, 201, 330, 793
MaeIII GTNAC 4 cut(s) 1299, 1718, 1915, 1942
MbiI CCGCTC 1 cut(s) 237
MboII GAAGA 7 cut(s) 115, 448, 549, 756, 1236, 1816, 2051
MfeI CAATTG 3 cut(s) 726, 1068, 2498
MflI RGATCY 4 cut(s) 538, 740, 761, 986
MhlI GDGCHC 5 cut(s) 260, 414, 695, 2370, 2543
MlsI TGGCCA 1 cut(s) 1307
MluNI TGGCCA 1 cut(s) 1307
Mly113I GGCGCC 2 cut(s) 179, 2014
MlyI GAGTC 9 cut(s) 101, 280, 563, 938, 1260, 1622, 1678, 2200, 2478
MmeI TCCRAC 4 cut(s) 75, 196, 483, 1593
Mox20I TGGCCA 1 cut(s) 1307
Mph1103I ATGCAT 1 cut(s) 1479
MreI CGCCGGCG 1 cut(s) 181
MroNI GCCGGC 1 cut(s) 181
MscI TGGCCA 1 cut(s) 1307
MslI CAYNNNNRTG 3 cut(s) 698, 773, 1472
Msp20I TGGCCA 1 cut(s) 1307
MspA1I CMGCKG 5 cut(s) 226, 472, 880, 1252, 2198
MspI CCGG 9 cut(s) 12, 172, 182, 267, 354, 417, 548, 1361, 2017
MspR9I CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2371
MunI CAATTG 3 cut(s) 726, 1068, 2498
MvaI CCWGG 5 cut(s) 753, 1188, 1992, 2317, 2371
MvnI CGCG 2 cut(s) 65, 281
MwoI GCNNNNNNNGC 8 cut(s) 74, 182, 243, 363, 515, 886, 1370, 1582
NaeI GCCGGC 1 cut(s) 183
NarI GGCGCC 2 cut(s) 179, 2014
NciI CCSGG 3 cut(s) 12, 173, 417
NcoI CCATGG 2 cut(s) 1712, 2543
NdeI CATATG 1 cut(s) 947
NgoMIV GCCGGC 1 cut(s) 181
NlaIII CATG 9 cut(s) 665, 1481, 1716, 1787, 1832, 2036, 2401, 2453, 2547
NlaIV GGNNCC 9 cut(s) 180, 411, 412, 413, 540, 830, 1659, 2015, 2223
NmuCI GTSAC 3 cut(s) 1299, 1718, 1942
NsiI ATGCAT 1 cut(s) 1479
NspI RCATGY 2 cut(s) 1481, 2453
OliI CACNNNNGTG 1 cut(s) 698
PaeR7I CTCGAG 1 cut(s) 1630
PagI TCATGA 1 cut(s) 2032
PasI CCCWGGG 1 cut(s) 2316
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdiI GCCGGC 1 cut(s) 183
PflFI GACNNNGTC 1 cut(s) 289
Ple19I CGATCG 2 cut(s) 790, 1489
PleI GAGTC 9 cut(s) 100, 280, 562, 937, 1260, 1622, 1678, 2200, 2477
PluTI GGCGCC 2 cut(s) 182, 2017
PpsI GAGTC 9 cut(s) 100, 280, 562, 937, 1260, 1622, 1678, 2200, 2477
PpuMI RGGWCCY 1 cut(s) 829
Psp124BI GAGCTC 1 cut(s) 260
Psp5II RGGWCCY 1 cut(s) 829
Psp6I CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2369
PspFI CCCAGC 2 cut(s) 441, 803
PspGI CCWGG 5 cut(s) 751, 1186, 1990, 2315, 2369
PspN4I GGNNCC 9 cut(s) 180, 411, 412, 413, 540, 830, 1659, 2015, 2223
PspOMI GGGCCC 1 cut(s) 410
PspPI GGNCC 5 cut(s) 410, 411, 829, 1223, 1994
PspPPI RGGWCCY 1 cut(s) 829
PspXI VCTCGAGB 1 cut(s) 1630
PsrI GAACNNNNNNTAC 2 cut(s) 895, 927
PstI CTGCAG 2 cut(s) 477, 2203
PstNI CAGNNNCTG 1 cut(s) 920
PsuI RGATCY 4 cut(s) 538, 740, 761, 986
PsyI GACNNNGTC 1 cut(s) 289
PvuI CGATCG 2 cut(s) 790, 1489
PvuII CAGCTG 3 cut(s) 472, 880, 2198
RseI CAYNNNNRTG 3 cut(s) 698, 773, 1472
SacI GAGCTC 1 cut(s) 260
SalI GTCGAC 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 431
Sau96I GGNCC 5 cut(s) 410, 411, 829, 1223, 1994
ScaI AGTACT 2 cut(s) 275, 1316
SchI GAGTC 9 cut(s) 101, 280, 563, 938, 1260, 1622, 1678, 2200, 2478
ScrFI CCNGG 8 cut(s) 12, 173, 417, 753, 1188, 1992, 2317, 2371
SduI GDGCHC 5 cut(s) 260, 414, 695, 2370, 2543
SfaNI GCATC 5 cut(s) 574, 604, 926, 1111, 1894
SfcI CTRYAG 5 cut(s) 473, 990, 1027, 1587, 2199
SfoI GGCGCC 2 cut(s) 180, 2015
Sfr274I CTCGAG 1 cut(s) 1630
SgrAI CRCCGGYG 1 cut(s) 181
SinI GGWCC 2 cut(s) 829, 1223
SlaI CTCGAG 1 cut(s) 1630
SmiMI CAYNNNNRTG 3 cut(s) 698, 773, 1472
SmlI CTYRAG 2 cut(s) 1630, 1852
SmoI CTYRAG 2 cut(s) 1630, 1852
SspDI GGCGCC 2 cut(s) 178, 2013
SspI AATATT 4 cut(s) 1106, 1894, 2286, 2344
SspMI CTAG 4 cut(s) 1058, 1662, 2391, 2442
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 8 cut(s) 10, 171, 415, 751, 1186, 1990, 2315, 2369
StyI CCWWGG 2 cut(s) 1712, 2543
TaaI ACNGT 9 cut(s) 1080, 1276, 1314, 1328, 1549, 1765, 2134, 2273, 2486
TaiI ACGT 4 cut(s) 83, 204, 333, 796
TaqII GACCGA 1 cut(s) 202
TatI WGTACW 7 cut(s) 204, 273, 1109, 1314, 1534, 1726, 2476
TauI GCSGC 6 cut(s) 68, 71, 165, 179, 229, 305
TscAI CASTG 4 cut(s) 549, 700, 967, 1308
TseFI GTSAC 3 cut(s) 1299, 1718, 1942
TseI GCWGC 8 cut(s) 223, 243, 448, 472, 897, 1008, 1252, 2198
Tsp45I GTSAC 3 cut(s) 1299, 1718, 1942
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 4 cut(s) 549, 700, 967, 1308
Tth111I GACNNNGTC 1 cut(s) 289
VneI GTGCAC 1 cut(s) 691
VpaK11BI GGWCC 2 cut(s) 829, 1223
XapI RAATTY 3 cut(s) 935, 1823, 2505
XceI RCATGY 2 cut(s) 1481, 2453
XhoI CTCGAG 1 cut(s) 1630
XmiI GTMKAC 5 cut(s) 293, 718, 1030, 1579, 2129
XspI CTAG 4 cut(s) 1058, 1662, 2391, 2442
ZraI GACGTC 1 cut(s) 81
ZrmI AGTACT 2 cut(s) 275, 1316
Zsp2I ATGCAT 1 cut(s) 1479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.