Rmu_sc0002532.1_g000019

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002532.1
OG0007466
Physical Location & Seq
Forward (+)
108254 .. 116486
8233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002532.1_g000019.1.cds

Sequence Viewer

Length: 2769 bp
atgcccggtaggagatcgaactacacgcttctcagccaagtccccgacgatcacttcgctgcggcgacggcgacgtcgttttacgagtcggaggggaagaacaacaataataaggcgaagggggatagcagagggtttgattgggaaacgggaggcggcgagtaccgggcggcgccggcgaatcggatcggaaacgtgtactcgtcggtcggattgcagcggcagtcgagcgggagcagcttcggagagagctcgctgtccggcgagtactacgcgccgactctgtcgaccacggcggcgaatgagattgacggattcgggtacgtcaacgacgacgggtttaaaaccggcggaggaggcggtgagttcagagggaaaggaggagggatggatgggggtgtgggcccccccggaggttcgtcgtcggggaagagctgggcgcagcagactgaggagagttatcagctgcagttggctttggcgcttcggctctcgtcggaagccacttgcgccgatgatcctaatttcttggatccagtgccggatgaatcttcgtcgaggtcgtccagctctgccgatgcagtttcgcatcgattttgggtgaatggctgtctgtcatactttgacaaagtgcctgatgggttttaccttattcatgggatagattcgtatgtatggtctatgtgcactgatgtgcaagagagtggtcgtataccatcaattgaatcactaaaatctgttgatcctggtactggatcttccatagaagtggtcttaatcgatcgacgtagtgatcccagcttaaaggagcttcaaaatagggtcctcagcatttcttatgcttgcataaccacgacagagattgttgatcagctggcaaagcttgtatgcagccgtatggggggttcagcatctgtaggagaggctgaatttttctccatatggagggagaccagtgatgatctaaaagattgcttaggatctgtagttgttccaatcggcagcctttctattggcctctgtagacatcgtgctttattattcaaggttctagctgacacaattgacctaccgtgccgaattgccaaaggctgtaaatattgtacaagagatgatgcttccacttgtcttgttcgttttggtattgacagggaattgttagttgatttgattgggaatccaggttgcttatgtgagcctgattccttgttcaatggtccatcatccatctcaatttcttcaccgctacggtttccaagactcagaacagttgaacctaccattgatttcaggtcactggccaaacagtacttctcggactgtcagttgcttaatcttgtattcgatgaagctccggcaggaagtgctggtgatgaggacaataaaggattctctatgtatcctaagcagaagtttacagatggaaacaacctctttctggtttcaggtcttggtgatgacacttctatgcatgttgacgatcggaatccccagttccttaaatcatttaatccttctcagaacattgtacaccaacaaacagtattaaaagatcaaatccccctaaagcgtataccgcctataggacatagagatatttcaaggttggatacaagtaaggactcgaggtttggtgagggacttcaagtggttcctagtaaaccaaataaagagctgactcttgatgtggatgatttggacattccatggagtgaccttgtactgaaggagagaatcggtgcaggttcttttggaactgttcatcgtgctgattggcatggctctgatgttgctgtgaagattcttatggagcaagaatttcatgccgaacgctttaaagagttcttgagggaggttacaattatgaaacgcctgcggcatccaaatattgtgctgtttatgggtgcagttacaaagcccccaaacttgtccatagtcacagaatatctatcaagagggagcttgtataggctgttgcataaacctggcccaatgctggatgagaggcgccggttgtatatggctcatgatgtggcaaaggggatgaattatcttcataggcggaatcctcccattgttcatcgagatttaaaatcgccaaaccttttggttgacaaaaagtatactgtgaaggtttgtgattttggtctttcccgattaaaggccaacacctttctttcatcaaaatcagctgcagggactcccgaatggatggcaccagaagtcctgcgtgatgaaccatcaaatgagaagtcagatgtttacagttttggtgtaatattgtgggaacttgcgacattgcaacagccctggggtaatttgaatccagcgcaggttgtggcagctgttggttttaagaacaaaaggcttgagattccacgtgatttgaatcctcaagtggcttctataattgaggcttgctgggccaatgagccctggaaacgaccttcatttgctagtatcatggaatctttgaggccattgattaaagctcccacacctcaacctagattacatgctgttcatagtctcggagtcattgtacattactgttcttatcgccaattgagaattctgattctttatgcaaatcaaggactaaagtgcccatgcctatgtggagactatgacgttgcgttccagttgaaccgcacagtcatgacgccgggtcgtgaagccgtgaaagatggggagggaagggagggagatctcagaaatcgagatctattcggggagcccaatgaatatgcagagcgagcatatttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

922

Amino Acids

101.81

Weight (kDa)

5.99

Isoelectric Point (pI)

44.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
AatII GACGTC 1 cut(s) 77
Acc36I ACCTGC 2 cut(s) 1733, 2323
AccB1I GGYRCC 3 cut(s) 172, 2007, 2215
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 5 cut(s) 287, 712, 1024, 1573, 2123
AccII CGCG 1 cut(s) 275
AclWI GGATC 8 cut(s) 194, 512, 527, 540, 737, 763, 788, 988
AcoI YGGCCR 1 cut(s) 1299
AcsI RAATTY 3 cut(s) 929, 1817, 2571
AcuI CTGAAG 1 cut(s) 1745
AcvI CACGTG 1 cut(s) 2381
AcyI GRCGYC 4 cut(s) 74, 173, 2008, 2663
AfiI CCNNNNNNNGG 8 cut(s) 413, 752, 901, 945, 1438, 1583, 1996, 2161
AflIII ACRYGT 1 cut(s) 195
AhdI GACNNNNNGTC 1 cut(s) 2667
AjnI CCWGG 5 cut(s) 745, 1180, 1984, 2309, 2435
AjuI GAANNNNNNNTTGG 4 cut(s) 1295, 1327, 2556, 2588
AleI CACNNNNGTG 1 cut(s) 692
Alw21I GWGCWC 2 cut(s) 254, 689
Alw26I GTCTC 3 cut(s) 944, 2534, 2616
Alw44I GTGCAC 1 cut(s) 685
AlwI GGATC 8 cut(s) 194, 512, 527, 540, 737, 763, 788, 988
AlwNI CAGNNNCTG 1 cut(s) 914
Ama87I CYCGRG 1 cut(s) 1624
AoxI GGCC 7 cut(s) 403, 1015, 1299, 1987, 2163, 2424, 2477
ApaI GGGCCC 1 cut(s) 407
ApaLI GTGCAC 1 cut(s) 685
ApeKI GCWGC 9 cut(s) 59, 217, 237, 442, 466, 891, 1002, 2192, 2342
ApoI RAATTY 3 cut(s) 929, 1817, 2571
ArsI GACNNNNNNTTYG 6 cut(s) 38, 70, 1613, 1645, 2237, 2269
AspLEI GCGC 7 cut(s) 175, 277, 442, 484, 512, 2010, 2332
AspS9I GGNCC 6 cut(s) 403, 404, 823, 1217, 1988, 2424
AsuC2I CCSGG 4 cut(s) 6, 167, 411, 2667
AsuHPI GGTGA 6 cut(s) 374, 613, 1233, 1382, 1466, 1646
AvaI CYCGRG 1 cut(s) 1624
AvaII GGWCC 2 cut(s) 823, 1217
BaeGI GKGCMC 3 cut(s) 407, 689, 2609
BalI TGGCCA 1 cut(s) 1301
BamHI GGATCC 1 cut(s) 532
BanI GGYRCC 3 cut(s) 172, 2007, 2215
BanII GRGCYC 4 cut(s) 254, 407, 2436, 2739
BarI GAAGNNNNNNTAC 2 cut(s) 742, 774
BbrPI CACGTG 1 cut(s) 2381
Bbv12I GWGCWC 2 cut(s) 254, 689
BbvCI CCTCAGC 1 cut(s) 827
BbvI GCAGC 9 cut(s) 46, 229, 249, 453, 454, 903, 1014, 2179, 2354
BceAI ACGGC 4 cut(s) 84, 309, 879, 2663
BciT130I CCWGG 5 cut(s) 747, 1182, 1986, 2311, 2437
BciVI GTATCC 2 cut(s) 1410, 1603
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 4 cut(s) 6, 167, 411, 2667
BcoDI GTCTC 3 cut(s) 944, 2534, 2616
BfaI CTAG 4 cut(s) 1052, 1656, 2457, 2508
BfmI CTRYAG 6 cut(s) 467, 915, 984, 1021, 1581, 2193
BfoI RGCGCY 3 cut(s) 176, 485, 2011
BfuAI ACCTGC 2 cut(s) 1733, 2323
BfuI GTATCC 2 cut(s) 1410, 1603
BglII AGATCT 2 cut(s) 2707, 2722
BmcAI AGTACT 2 cut(s) 269, 1310
Bme1390I CCNGG 9 cut(s) 6, 167, 411, 747, 1182, 1986, 2311, 2437, 2667
Bme18I GGWCC 2 cut(s) 823, 1217
BmeRI GACNNNNNGTC 1 cut(s) 2667
BmeT110I CYCGRG 1 cut(s) 1624
BmgT120I GGNCC 6 cut(s) 403, 404, 823, 1217, 1988, 2424
BmiI GGNNCC 9 cut(s) 174, 405, 406, 534, 824, 1653, 2009, 2217, 2736
BmrFI CCNGG 9 cut(s) 6, 167, 411, 747, 1182, 1986, 2311, 2437, 2667
BmrI ACTGGG 1 cut(s) 1486
BmsI GCATC 5 cut(s) 568, 598, 920, 1105, 1888
BmuI ACTGGG 1 cut(s) 1486
Bpu10I CCTNAGC 3 cut(s) 827, 976, 1404
BpuEI CTTGAG 3 cut(s) 1867, 2379, 2390
BpuMI CCSGG 4 cut(s) 6, 167, 411, 2667
Bsa29I ATCGAT 2 cut(s) 592, 780
BsaAI YACGTR 1 cut(s) 2381
BsaBI GATNNNNATC 2 cut(s) 1485, 2388
BsaHI GRCGYC 4 cut(s) 74, 173, 2008, 2663
BsaI GGTCTC 1 cut(s) 944
BsaJI CCNNGG 6 cut(s) 291, 409, 1706, 2309, 2310, 2435
BsaXI ACNNNNNCTCC 2 cut(s) 2526, 2556
Bsc4I CCNNNNNNNGG 8 cut(s) 413, 752, 901, 945, 1438, 1583, 1996, 2161
Bse118I RCCGGY 3 cut(s) 175, 347, 2010
Bse1I ACTGG 6 cut(s) 536, 757, 954, 1302, 1492, 2641
Bse3DI GCAATG 1 cut(s) 2297
Bse8I GATNNNNATC 2 cut(s) 1485, 2388
BseBI CCWGG 5 cut(s) 747, 1182, 1986, 2311, 2437
BseCI ATCGAT 2 cut(s) 592, 780
BseDI CCNNGG 6 cut(s) 291, 409, 1706, 2309, 2310, 2435
BseGI GGATG 9 cut(s) 393, 397, 550, 1223, 1696, 1879, 2005, 2049, 2217
BseJI GATNNNNATC 2 cut(s) 1485, 2388
BseLI CCNNNNNNNGG 8 cut(s) 413, 752, 901, 945, 1438, 1583, 1996, 2161
BseMI GCAATG 1 cut(s) 2297
BseMII CTCAG 6 cut(s) 46, 441, 841, 1276, 1532, 2725
BseNI ACTGG 6 cut(s) 536, 757, 954, 1302, 1492, 2641
BseRI GAGGAG 3 cut(s) 369, 396, 467
BseSI GKGCMC 3 cut(s) 407, 689, 2609
BseXI GCAGC 9 cut(s) 46, 229, 249, 453, 454, 903, 1014, 2179, 2354
BseYI CCCAGC 3 cut(s) 435, 797, 2421
BsgI GTGCAG 2 cut(s) 1761, 1926
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 3 cut(s) 210, 784, 1483
BshFI GGCC 7 cut(s) 405, 1017, 1301, 1989, 2165, 2426, 2479
BshNI GGYRCC 3 cut(s) 172, 2007, 2215
BshVI ATCGAT 2 cut(s) 592, 780
BsiEI CGRYCG 3 cut(s) 210, 784, 1483
BsiHKAI GWGCWC 2 cut(s) 254, 689
BsiHKCI CYCGRG 1 cut(s) 1624
BslFI GGGAC 3 cut(s) 26, 1653, 2212
BslI CCNNNNNNNGG 8 cut(s) 413, 752, 901, 945, 1438, 1583, 1996, 2161
BsmAI GTCTC 3 cut(s) 944, 2534, 2616
BsmFI GGGAC 3 cut(s) 26, 1653, 2212
BsnI GGCC 7 cut(s) 405, 1017, 1301, 1989, 2165, 2426, 2479
Bso31I GGTCTC 1 cut(s) 944
BsoBI CYCGRG 1 cut(s) 1624
Bsp120I GGGCCC 1 cut(s) 403
Bsp1286I GDGCHC 6 cut(s) 254, 407, 689, 2436, 2609, 2739
Bsp1407I TGTACA 3 cut(s) 1103, 1528, 2542
Bsp19I CCATGG 1 cut(s) 1706
BspANI GGCC 7 cut(s) 405, 1017, 1301, 1989, 2165, 2426, 2479
BspCNI CTCAG 6 cut(s) 45, 442, 840, 1275, 1531, 2724
BspDI ATCGAT 2 cut(s) 592, 780
BspFNI CGCG 1 cut(s) 275
BspHI TCATGA 2 cut(s) 2026, 2658
BspLI GGNNCC 9 cut(s) 174, 405, 406, 534, 824, 1653, 2009, 2217, 2736
BspMAI CTGCAG 2 cut(s) 471, 2197
BspMI ACCTGC 2 cut(s) 1733, 2323
BspPI GGATC 8 cut(s) 194, 512, 527, 540, 737, 763, 788, 988
BspQI GCTCTTC 1 cut(s) 425
BspT107I GGYRCC 3 cut(s) 172, 2007, 2215
BspTNI GGTCTC 1 cut(s) 944
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 1 cut(s) 2297
BsrFI RCCGGY 3 cut(s) 175, 347, 2010
BsrGI TGTACA 3 cut(s) 1103, 1528, 2542
BsrI ACTGG 6 cut(s) 536, 757, 954, 1302, 1492, 2641
BssAI RCCGGY 3 cut(s) 175, 347, 2010
BssECI CCNNGG 6 cut(s) 291, 409, 1706, 2309, 2310, 2435
BssNAI GTATAC 3 cut(s) 713, 1574, 2124
BssNI GRCGYC 4 cut(s) 74, 173, 2008, 2663
BssT1I CCWWGG 1 cut(s) 1706
Bst1107I GTATAC 3 cut(s) 713, 1574, 2124
Bst2UI CCWGG 5 cut(s) 747, 1182, 1986, 2311, 2437
Bst6I CTCTTC 1 cut(s) 425
BstACI GRCGYC 4 cut(s) 74, 173, 2008, 2663
BstAPI GCANNNNNTGC 1 cut(s) 1364
BstAUI TGTACA 3 cut(s) 1103, 1528, 2542
BstBAI YACGTR 1 cut(s) 2381
BstC8I GCNNGC 7 cut(s) 177, 254, 844, 876, 1874, 2419, 2757
BstDEI CTNAG 8 cut(s) 32, 450, 827, 976, 1262, 1404, 1518, 2711
BstDSI CCRYGG 2 cut(s) 291, 1706
BstF5I GGATG 9 cut(s) 393, 397, 550, 1223, 1696, 1879, 2005, 2049, 2217
BstFNI CGCG 1 cut(s) 275
BstH2I RGCGCY 3 cut(s) 176, 485, 2011
BstHHI GCGC 7 cut(s) 175, 277, 442, 484, 512, 2010, 2332
BstMAI GTCTC 3 cut(s) 944, 2534, 2616
BstMCI CGRYCG 3 cut(s) 210, 784, 1483
BstNI CCWGG 5 cut(s) 747, 1182, 1986, 2311, 2437
BstNSI RCATGY 2 cut(s) 1475, 2519
BstSCI CCNGG 9 cut(s) 4, 165, 409, 745, 1180, 1984, 2309, 2435, 2665
BstSFI CTRYAG 6 cut(s) 467, 915, 984, 1021, 1581, 2193
BstSLI GKGCMC 3 cut(s) 407, 689, 2609
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 9 cut(s) 46, 229, 249, 453, 454, 903, 1014, 2179, 2354
BstX2I RGATCY 5 cut(s) 532, 755, 980, 2707, 2722
BstXI CCANNNNNNTGG 1 cut(s) 769
BstYI RGATCY 5 cut(s) 532, 755, 980, 2707, 2722
BstZ17I GTATAC 3 cut(s) 713, 1574, 2124
Bsu15I ATCGAT 2 cut(s) 592, 780
BsuI GTATCC 2 cut(s) 1410, 1603
BsuRI GGCC 7 cut(s) 405, 1017, 1301, 1989, 2165, 2426, 2479
BsuTUI ATCGAT 2 cut(s) 592, 780
BtgI CCRYGG 2 cut(s) 291, 1706
BtsCI GGATG 9 cut(s) 393, 397, 550, 1223, 1696, 1879, 2005, 2049, 2217
BtsIMutI CAGTG 4 cut(s) 543, 687, 961, 1295
BveI ACCTGC 2 cut(s) 1733, 2323
Cac8I GCNNGC 7 cut(s) 177, 254, 844, 876, 1874, 2419, 2757
CaiI CAGNNNCTG 1 cut(s) 914
CciI TCATGA 2 cut(s) 2026, 2658
CfoI GCGC 7 cut(s) 175, 277, 442, 484, 512, 2010, 2332
Cfr10I RCCGGY 3 cut(s) 175, 347, 2010
Cfr13I GGNCC 6 cut(s) 403, 404, 823, 1217, 1988, 2424
ClaI ATCGAT 2 cut(s) 592, 780
CseI GACGC 1 cut(s) 2671
CspCI CAANNNNNGTGG 4 cut(s) 1111, 1146, 2367, 2402
DdeI CTNAG 8 cut(s) 32, 450, 827, 976, 1262, 1404, 1518, 2711
DinI GGCGCC 2 cut(s) 174, 2009
DraI TTTAAA 3 cut(s) 343, 1837, 2091
DrdI GACNNNNNNGTC 1 cut(s) 73
DriI GACNNNNNGTC 1 cut(s) 2667
DseDI GACNNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 1299
Eam1104I CTCTTC 1 cut(s) 425
Eam1105I GACNNNNNGTC 1 cut(s) 2667
EarI CTCTTC 1 cut(s) 425
EciI GGCGGA 2 cut(s) 366, 2077
Ecl136II GAGCTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 1706
Eco24I GRGCYC 4 cut(s) 254, 407, 2436, 2739
Eco31I GGTCTC 1 cut(s) 944
Eco47I GGWCC 2 cut(s) 823, 1217
Eco53kI GAGCTC 1 cut(s) 252
Eco57I CTGAAG 1 cut(s) 1745
Eco72I CACGTG 1 cut(s) 2381
Eco88I CYCGRG 1 cut(s) 1624
EcoICRI GAGCTC 1 cut(s) 252
EcoO109I RGGNCCY 2 cut(s) 404, 823
EcoRI GAATTC 1 cut(s) 2571
EcoRII CCWGG 5 cut(s) 745, 1180, 1984, 2309, 2435
EcoT14I CCWWGG 1 cut(s) 1706
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 4 cut(s) 254, 407, 2436, 2739
EgeI GGCGCC 2 cut(s) 174, 2009
EheI GGCGCC 2 cut(s) 174, 2009
ErhI CCWWGG 1 cut(s) 1706
FaqI GGGAC 3 cut(s) 26, 1653, 2212
FauI CCCGC 1 cut(s) 224
FauNDI CATATG 1 cut(s) 941
FbaI TGATCA 1 cut(s) 868
FblI GTMKAC 5 cut(s) 287, 712, 1024, 1573, 2123
FokI GGATG 9 cut(s) 400, 404, 557, 1210, 1703, 1866, 2012, 2056, 2224
FriOI GRGCYC 4 cut(s) 254, 407, 2436, 2739
FspBI CTAG 4 cut(s) 1052, 1656, 2457, 2508
GlaI GCGC 7 cut(s) 174, 276, 441, 483, 511, 2009, 2331
GsaI CCCAGC 3 cut(s) 439, 801, 2425
HaeII RGCGCY 3 cut(s) 176, 485, 2011
HaeIII GGCC 7 cut(s) 405, 1017, 1301, 1989, 2165, 2426, 2479
HgaI GACGC 1 cut(s) 2671
HhaI GCGC 7 cut(s) 175, 277, 442, 484, 512, 2010, 2332
Hin1I GRCGYC 4 cut(s) 74, 173, 2008, 2663
Hin6I GCGC 7 cut(s) 173, 275, 440, 482, 510, 2008, 2330
HinP1I GCGC 7 cut(s) 173, 275, 440, 482, 510, 2008, 2330
HincII GTYRAC 4 cut(s) 288, 328, 1477, 2113
HindII GTYRAC 4 cut(s) 288, 328, 1477, 2113
HindIII AAGCTT 1 cut(s) 881
HphI GGTGA 6 cut(s) 374, 613, 1233, 1382, 1466, 1646
HpyAV CCTTC 6 cut(s) 112, 1524, 1720, 2125, 2457, 2691
HpyCH4IV ACGT 6 cut(s) 74, 195, 324, 787, 2380, 2631
HpyF3I CTNAG 8 cut(s) 32, 450, 827, 976, 1262, 1404, 1518, 2711
HpySE526I ACGT 6 cut(s) 74, 195, 324, 787, 2380, 2631
Hsp92I GRCGYC 4 cut(s) 74, 173, 2008, 2663
HspAI GCGC 7 cut(s) 173, 275, 440, 482, 510, 2008, 2330
KasI GGCGCC 2 cut(s) 172, 2007
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
Ksp22I TGATCA 1 cut(s) 868
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 7 cut(s) 234, 808, 1357, 1810, 1959, 2497, 2734
Lsp1109I GCAGC 9 cut(s) 46, 229, 249, 453, 454, 903, 1014, 2179, 2354
LweI GCATC 5 cut(s) 568, 598, 920, 1105, 1888
MaeI CTAG 4 cut(s) 1052, 1656, 2457, 2508
MaeII ACGT 6 cut(s) 74, 195, 324, 787, 2380, 2631
MaeIII GTNAC 5 cut(s) 1293, 1712, 1855, 1909, 1936
MbiI CCGCTC 1 cut(s) 231
MboII GAAGA 7 cut(s) 109, 442, 543, 750, 1230, 1810, 2045
MfeI CAATTG 3 cut(s) 720, 1062, 2564
MflI RGATCY 5 cut(s) 532, 755, 980, 2707, 2722
MhlI GDGCHC 6 cut(s) 254, 407, 689, 2436, 2609, 2739
MlsI TGGCCA 1 cut(s) 1301
MluNI TGGCCA 1 cut(s) 1301
Mly113I GGCGCC 2 cut(s) 173, 2008
MlyI GAGTC 7 cut(s) 95, 274, 1254, 1616, 1672, 2194, 2544
MmeI TCCRAC 4 cut(s) 69, 190, 477, 1587
Mox20I TGGCCA 1 cut(s) 1301
Mph1103I ATGCAT 1 cut(s) 1473
MreI CGCCGGCG 1 cut(s) 175
MroNI GCCGGC 1 cut(s) 175
MscI TGGCCA 1 cut(s) 1301
MslI CAYNNNNRTG 5 cut(s) 692, 767, 1466, 2614, 2657
Msp20I TGGCCA 1 cut(s) 1301
MspA1I CMGCKG 5 cut(s) 220, 466, 874, 2192, 2345
MspR9I CCNGG 9 cut(s) 6, 167, 411, 747, 1182, 1986, 2311, 2437, 2667
MunI CAATTG 3 cut(s) 720, 1062, 2564
MvaI CCWGG 5 cut(s) 747, 1182, 1986, 2311, 2437
MvnI CGCG 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 177
NarI GGCGCC 2 cut(s) 173, 2008
NciI CCSGG 4 cut(s) 6, 167, 411, 2667
NcoI CCATGG 1 cut(s) 1706
NdeI CATATG 1 cut(s) 941
NgoMIV GCCGGC 1 cut(s) 175
NlaIV GGNNCC 9 cut(s) 174, 405, 406, 534, 824, 1653, 2009, 2217, 2736
NmuCI GTSAC 3 cut(s) 1293, 1712, 1936
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 2 cut(s) 1475, 2519
OliI CACNNNNGTG 1 cut(s) 692
PaeR7I CTCGAG 1 cut(s) 1624
PagI TCATGA 2 cut(s) 2026, 2658
PasI CCCWGGG 1 cut(s) 2310
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 3 cut(s) 74, 330, 560
PdiI GCCGGC 1 cut(s) 177
PflFI GACNNNGTC 1 cut(s) 283
Ple19I CGATCG 2 cut(s) 784, 1483
PleI GAGTC 7 cut(s) 94, 274, 1254, 1616, 1672, 2194, 2543
PluTI GGCGCC 2 cut(s) 176, 2011
PmaCI CACGTG 1 cut(s) 2381
PmlI CACGTG 1 cut(s) 2381
PpsI GAGTC 7 cut(s) 94, 274, 1254, 1616, 1672, 2194, 2543
Ppu21I YACGTR 1 cut(s) 2381
PpuMI RGGWCCY 1 cut(s) 823
Psp124BI GAGCTC 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 5 cut(s) 745, 1180, 1984, 2309, 2435
PspCI CACGTG 1 cut(s) 2381
PspFI CCCAGC 3 cut(s) 435, 797, 2421
PspGI CCWGG 5 cut(s) 745, 1180, 1984, 2309, 2435
PspN4I GGNNCC 9 cut(s) 174, 405, 406, 534, 824, 1653, 2009, 2217, 2736
PspOMI GGGCCC 1 cut(s) 403
PspPI GGNCC 6 cut(s) 403, 404, 823, 1217, 1988, 2424
PspPPI RGGWCCY 1 cut(s) 823
PspXI VCTCGAGB 1 cut(s) 1624
PsrI GAACNNNNNNTAC 2 cut(s) 889, 921
PstI CTGCAG 2 cut(s) 471, 2197
PstNI CAGNNNCTG 1 cut(s) 914
PsuI RGATCY 5 cut(s) 532, 755, 980, 2707, 2722
PsyI GACNNNGTC 1 cut(s) 283
PvuI CGATCG 2 cut(s) 784, 1483
PvuII CAGCTG 4 cut(s) 466, 874, 2192, 2345
RseI CAYNNNNRTG 5 cut(s) 692, 767, 1466, 2614, 2657
SacI GAGCTC 1 cut(s) 254
SalI GTCGAC 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 425
Sau96I GGNCC 6 cut(s) 403, 404, 823, 1217, 1988, 2424
ScaI AGTACT 2 cut(s) 269, 1310
SchI GAGTC 7 cut(s) 95, 274, 1254, 1616, 1672, 2194, 2544
ScrFI CCNGG 9 cut(s) 6, 167, 411, 747, 1182, 1986, 2311, 2437, 2667
SduI GDGCHC 6 cut(s) 254, 407, 689, 2436, 2609, 2739
SfaNI GCATC 5 cut(s) 568, 598, 920, 1105, 1888
SfcI CTRYAG 6 cut(s) 467, 915, 984, 1021, 1581, 2193
SfoI GGCGCC 2 cut(s) 174, 2009
Sfr274I CTCGAG 1 cut(s) 1624
SgrAI CRCCGGYG 1 cut(s) 175
SinI GGWCC 2 cut(s) 823, 1217
SlaI CTCGAG 1 cut(s) 1624
SmiMI CAYNNNNRTG 5 cut(s) 692, 767, 1466, 2614, 2657
SmlI CTYRAG 4 cut(s) 1624, 1846, 2369, 2394
SmoI CTYRAG 4 cut(s) 1624, 1846, 2369, 2394
SspDI GGCGCC 2 cut(s) 172, 2007
SspI AATATT 3 cut(s) 1100, 1888, 2280
SspMI CTAG 4 cut(s) 1052, 1656, 2457, 2508
SstI GAGCTC 1 cut(s) 254
StyD4I CCNGG 9 cut(s) 4, 165, 409, 745, 1180, 1984, 2309, 2435, 2665
StyI CCWWGG 1 cut(s) 1706
TaiI ACGT 6 cut(s) 77, 198, 327, 790, 2383, 2634
TaqII GACCGA 1 cut(s) 196
TatI WGTACW 7 cut(s) 198, 267, 1103, 1308, 1528, 1720, 2542
TauI GCSGC 6 cut(s) 65, 159, 173, 223, 299, 1879
TscAI CASTG 4 cut(s) 543, 694, 961, 1302
TseFI GTSAC 3 cut(s) 1293, 1712, 1936
TseI GCWGC 9 cut(s) 59, 217, 237, 442, 466, 891, 1002, 2192, 2342
Tsp45I GTSAC 3 cut(s) 1293, 1712, 1936
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 4 cut(s) 543, 694, 961, 1302
Tth111I GACNNNGTC 1 cut(s) 283
VneI GTGCAC 1 cut(s) 685
VpaK11BI GGWCC 2 cut(s) 823, 1217
XapI RAATTY 3 cut(s) 929, 1817, 2571
XceI RCATGY 2 cut(s) 1475, 2519
XhoI CTCGAG 1 cut(s) 1624
XmiI GTMKAC 5 cut(s) 287, 712, 1024, 1573, 2123
XspI CTAG 4 cut(s) 1052, 1656, 2457, 2508
ZraI GACGTC 1 cut(s) 75
ZrmI AGTACT 2 cut(s) 269, 1310
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.