MD14G1015800.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0007466
Physical Location & Seq
Forward (+)
1410246 .. 1422637
12392 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1015800.v1.1.491

Sequence Viewer

Length: 2343 bp
ATGGAAATGCCCGGTAGGAGATCCAACTACAAGCTTCTGAGCCAAGTCCCCGACGACCAGACGGCGGCGTTTTACGAGACGGAGAGCAAGAACAAGGGGAAATGCGACAGCGGGCTCGATTGGGTAACCGGAGCCGATTACCGGGCGAATCAGCAAGCGATTCGTGCCGGGAACCCGTACTCGTCGGCCTGGTTGCAGAGGCAGTCGAGTGGGTTCGGGTACGTGCCGGACGATGTGTTCAAGGTTAGTGGTGTAGGGGGTGAATTTCGAATGAAGGGTGTGCATGGAGCTGTTGCGGTCACCAGCGGTGGCGGCGGCTCGTCTTCTTGGAAGAGCTGGGCTCAGCAGACTGAGGAGAGTTATCAGCTGCAGCTGGCTTTGGCTCTTCGGCTCTCATCGGAGGCCACATGCGCCGATGATCCTAACTTCTTGGATCCAGTGCTCGATGAATCAATGTCTAGGACATGGGGCTCCGTTGACGCCATTTCGCATCGGTTTTGGGTGAATGGCTGCCTGTCGTACTTTGACAAAGTGCCTGATGGATTTTACCTAATTCATGGAATAGATCAGTATGTATGGACTGTGTGCACTGATCTGAAAGAAAATGGTCGTATACCATCAATTGATTCATTAAGGTCTGTTGATCCTGGCATTGGTTCAAGTATTGAAGTGGTTTTAATTGATCGACGTAGTGATCCCAGTCTAAAGGAGCTCCAAAATAGGGTTTTAACCATTTCTCGTACCTGCATAACTATGAAAGAGATTGTAGATCAGATGGCAAAGCTTGTATGCAGTCGCATGGGGGGTTCAGCTTCTGTAGGAGAGGATGAAATATTGTCCATATGGAGGGAGAGCAGTGATGATCTGAAAGATTGCGGATCTGTAGTTGTTCCAATAGGCAGCCTTTCTGTTGGCCTATGTAGACATCGTGCTTTATTATTCAAAGTTCTGGCTGATACAATTGGTTTACCTTGTCGAATTGCCAAAGGCTGTAAATGTTGTACAAGGGATAATGCCTCCTCTTGTCTTGTTCGGTTTGAGAGGGACAGGGAGTATTTAGTCGATTTGATTGCAAATCCAGGTTACTTGTGCGAGCCTGATTCCTTGCTCAATGGCCCATCATCCATCTCAATTTCTTCACCGCTGCACTTTCCAAGACTAAAACCAGTTGAACCTACTATTGATTTCAGGTTACTGGCTAATCAGTATTTCTCGGACTGTCAATCGCTTAATCTTGTTTTTGATGAAGCTTCTGCAGGAAGTGCTGTTGATGAGGACAATATGTATCCTAAACAATTTGATAGGAAGAGTACAGAGGGAAAAAACCTTTTTTCAAGTTTCAGTGGCTACACCTCTGCACACGCAGAAATTCCTGAGACAAGTGGGCATAGAGATGGCCCAAAGGTGGATACAATTACAGACTCAAGGTTTGTTGAGGGTGTTCGACTGGTTCCTAGTAAACCAAGTAAAGAGCTCGCACTCAATGTAGGGAATTTGGACATCCCATGGAGTGATCTCGTAATAAAAGAGAAAATTGGTGCAGGTTCTTTTGGAACTGTTCAACGTGCTGATTGGCATGGCTCTGATGTTGCTGTAAAGATTCTTATGGAGCAAGATTTACATGAAGAACACTTTAAAGAATTTTTGAGTGAGGTTACTATAATGAAGGACCTGCGGCATCCAAACATTGTTCTCTTTATGGGTGCAGTTACCAAGCCGCCAAACTTGTCCATAGTCACGGAATATTTATCAAGAGGTAGCTTGTTTACGCTTTTGCAGAAACCTGGTGCAAGGGAGGCATTGGATGAGAACAGCCGCTTGAAAATGGCTCATGATGTGGCAAAGGGAATGAATTATCTTCATAGACACAAACCTCCCATCGTTCATCATGATTTAAAATCTCCAAATCTGTTGGTAGACAAACATTATACAGTGAAGGTTTGCGATTTTGGTCTTTCCCGTTCAAAGGCAAGCACCTTCCTTTCCCCAAAATCAGCTGCTGGGACTGCTGAATGGATGGCACCAGAAGTCATGCGTAATGAACCGTCAAATGAGAAGTCAGATGTTTACAGTTTTGGTGTAATATTGTGGGAACTCGCAACATTGCAACGGCCGTGGGGTAATTTAAATCCACTGCAGGTTGTCTCAGCTGTTGTGGTTTATAACAAAAGGCTGGACATTCCGCATGATGTGAATCCACGTGTGGCGGCTATAATTGAGGCTTGCTGGGCCAACGAACCCTGGAAACGACCTTCATTTGCTAGTATCATGGAATCTTTGACGCCATTGATAAAAGATCCTGTGACTCAACCTAGTCATGCAAATATGCCATTACTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 map04016 map04075
Pfam Domains
Protein Families
EDR1 Pkinase_Tyr

Protein Analysis

781

Amino Acids

86.3

Weight (kDa)

5.87

Isoelectric Point (pI)

38.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EDR1_CTR1_ARMC3_pept PF14381 159 - 364 9.9e-79 EDR1/CTR1/ARMC3, peptidase-like
PK_Tyr_Ser-Thr PF07714 507 - 760 4.9e-67 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 508 - 758 1e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2163
Acc36I ACCTGC 4 cut(s) 752, 1532, 1680, 2128
AccB1I GGYRCC 1 cut(s) 2020
AccI GTMKAC 3 cut(s) 613, 922, 1917
AclWI GGATC 8 cut(s) 15, 413, 428, 441, 638, 689, 886, 2291
AcoI YGGCCR 1 cut(s) 2111
AcsI RAATTY 4 cut(s) 263, 1368, 1492, 1640
AcvI CACGTG 1 cut(s) 2201
AcyI GRCGYC 2 cut(s) 480, 2282
AfaI GTAC 6 cut(s) 179, 221, 521, 742, 1003, 1312
AfiI CCNNNNNNNGG 7 cut(s) 64, 141, 653, 721, 846, 1405, 1966
AflIII ACRYGT 1 cut(s) 2200
AgsI TTSAA 9 cut(s) 241, 660, 668, 943, 1172, 1335, 1562, 1822, 1965
AjnI CCWGG 5 cut(s) 188, 646, 1078, 1783, 2240
AloI GAACNNNNNNTCC 2 cut(s) 221, 253
Alw21I GWGCWC 4 cut(s) 444, 590, 714, 1476
Alw26I GTCTC 3 cut(s) 71, 1370, 2149
Alw44I GTGCAC 1 cut(s) 586
AlwI GGATC 8 cut(s) 15, 413, 428, 441, 638, 689, 886, 2291
AlwNI CAGNNNCTG 2 cut(s) 815, 2000
AoxI GGCC 7 cut(s) 186, 402, 913, 1114, 1396, 2111, 2229
ApaLI GTGCAC 1 cut(s) 586
ApeKI GCWGC 6 cut(s) 367, 370, 510, 900, 1144, 1997
ApoI RAATTY 4 cut(s) 263, 1368, 1492, 1640
ArsI GACNNNNNNTTYG 4 cut(s) 1412, 1444, 2042, 2074
AspLEI GCGC 1 cut(s) 413
AspS9I GGNCC 4 cut(s) 1115, 1397, 1669, 2229
AsuC2I CCSGG 3 cut(s) 12, 143, 169
AsuHPI GGTGA 5 cut(s) 272, 292, 514, 1131, 2329
AsuII TTCGAA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 1669
BaeGI GKGCMC 1 cut(s) 590
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 2020
BanII GRGCYC 5 cut(s) 117, 343, 473, 714, 1476
BbrPI CACGTG 1 cut(s) 2201
BbsI GAAGAC 1 cut(s) 315
Bbv12I GWGCWC 4 cut(s) 444, 590, 714, 1476
BbvI GCAGC 6 cut(s) 354, 382, 497, 912, 1131, 1984
BccI CCATC 8 cut(s) 533, 625, 769, 1126, 1133, 1388, 1886, 2011
BceAI ACGGC 3 cut(s) 78, 2098, 2126
BcgI CGANNNNNNTGC 4 cut(s) 143, 177, 1052, 1086
BciT130I CCWGG 5 cut(s) 190, 648, 1080, 1785, 2242
BciVI GTATCC 2 cut(s) 1296, 1402
BcnI CCSGG 3 cut(s) 12, 143, 169
BcoDI GTCTC 3 cut(s) 71, 1370, 2149
BfaI CTAG 4 cut(s) 459, 1455, 2262, 2313
BfmI CTRYAG 5 cut(s) 368, 816, 882, 1254, 2135
BfuAI ACCTGC 4 cut(s) 752, 1532, 1680, 2128
BfuI GTATCC 2 cut(s) 1296, 1402
BlpI GCTNAGC 1 cut(s) 342
Bme1390I CCNGG 8 cut(s) 12, 143, 169, 190, 648, 1080, 1785, 2242
Bme18I GGWCC 1 cut(s) 1669
BmgT120I GGNCC 4 cut(s) 1115, 1397, 1669, 2229
BmiI GGNNCC 6 cut(s) 133, 173, 435, 472, 1452, 2022
BmrFI CCNGG 8 cut(s) 12, 143, 169, 190, 648, 1080, 1785, 2242
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 2 cut(s) 499, 1687
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 315
BplI GAGNNNNNCTC 2 cut(s) 325, 357
Bpu1102I GCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 1408
BpuMI CCSGG 3 cut(s) 12, 143, 169
BsaAI YACGTR 2 cut(s) 223, 2201
BsaBI GATNNNNATC 1 cut(s) 2193
BsaHI GRCGYC 2 cut(s) 480, 2282
BsaJI CCNNGG 3 cut(s) 1505, 2114, 2240
BsaWI WCCGGW 1 cut(s) 128
Bsc4I CCNNNNNNNGG 7 cut(s) 64, 141, 653, 721, 846, 1405, 1966
Bse1I ACTGG 5 cut(s) 437, 699, 1166, 1200, 1452
Bse3DI GCAATG 1 cut(s) 2102
Bse8I GATNNNNATC 1 cut(s) 2193
BseBI CCWGG 5 cut(s) 190, 648, 1080, 1785, 2242
BseDI CCNNGG 3 cut(s) 1505, 2114, 2240
BseGI GGATG 6 cut(s) 832, 1121, 1500, 1678, 1810, 2022
BseJI GATNNNNATC 1 cut(s) 2193
BseLI CCNNNNNNNGG 7 cut(s) 64, 141, 653, 721, 846, 1405, 1966
BseMI GCAATG 1 cut(s) 2102
BseMII CTCAG 5 cut(s) 29, 342, 356, 1365, 2160
BseNI ACTGG 5 cut(s) 437, 699, 1166, 1200, 1452
BseRI GAGGAG 2 cut(s) 368, 1009
BseSI GKGCMC 1 cut(s) 590
BseX3I CGGCCG 1 cut(s) 2111
BseXI GCAGC 6 cut(s) 354, 382, 497, 912, 1131, 1984
BseYI CCCAGC 3 cut(s) 336, 2000, 2226
BsgI GTGCAG 4 cut(s) 1130, 1341, 1560, 1725
Bsh1285I CGRYCG 1 cut(s) 2114
BshFI GGCC 7 cut(s) 188, 404, 915, 1116, 1398, 2113, 2231
BshNI GGYRCC 1 cut(s) 2020
BsiEI CGRYCG 1 cut(s) 2114
BsiHKAI GWGCWC 4 cut(s) 444, 590, 714, 1476
BsiSI CCGG 5 cut(s) 12, 129, 142, 168, 227
BslFI GGGAC 3 cut(s) 32, 1058, 2017
BslI CCNNNNNNNGG 7 cut(s) 64, 141, 653, 721, 846, 1405, 1966
BsmAI GTCTC 3 cut(s) 71, 1370, 2149
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 3 cut(s) 32, 1058, 2017
BsnI GGCC 7 cut(s) 188, 404, 915, 1116, 1398, 2113, 2231
Bsp119I TTCGAA 1 cut(s) 268
Bsp1286I GDGCHC 7 cut(s) 117, 343, 444, 473, 590, 714, 1476
Bsp1407I TGTACA 1 cut(s) 1001
Bsp1720I GCTNAGC 1 cut(s) 342
Bsp19I CCATGG 1 cut(s) 1505
BspANI GGCC 7 cut(s) 188, 404, 915, 1116, 1398, 2113, 2231
BspCNI CTCAG 5 cut(s) 30, 343, 355, 1366, 2159
BspHI TCATGA 2 cut(s) 1831, 1888
BspLI GGNNCC 6 cut(s) 133, 173, 435, 472, 1452, 2022
BspMAI CTGCAG 3 cut(s) 372, 1258, 2139
BspMI ACCTGC 4 cut(s) 752, 1532, 1680, 2128
BspPI GGATC 8 cut(s) 15, 413, 428, 441, 638, 689, 886, 2291
BspQI GCTCTTC 2 cut(s) 326, 390
BspT104I TTCGAA 1 cut(s) 268
BspT107I GGYRCC 1 cut(s) 2020
BsrDI GCAATG 1 cut(s) 2102
BsrGI TGTACA 1 cut(s) 1001
BsrI ACTGG 5 cut(s) 437, 699, 1166, 1200, 1452
BssECI CCNNGG 3 cut(s) 1505, 2114, 2240
BssNAI GTATAC 1 cut(s) 614
BssNI GRCGYC 2 cut(s) 480, 2282
BssT1I CCWWGG 1 cut(s) 1505
Bst1107I GTATAC 1 cut(s) 614
Bst2UI CCWGG 5 cut(s) 190, 648, 1080, 1785, 2242
Bst4CI ACNGT 6 cut(s) 583, 1220, 1558, 1933, 2046, 2072
Bst6I CTCTTC 3 cut(s) 326, 390, 1301
BstACI GRCGYC 2 cut(s) 480, 2282
BstAPI GCANNNNNTGC 1 cut(s) 1262
BstAUI TGTACA 1 cut(s) 1001
BstBAI YACGTR 2 cut(s) 223, 2201
BstBI TTCGAA 1 cut(s) 268
BstC8I GCNNGC 7 cut(s) 113, 156, 375, 1094, 1476, 1972, 2224
BstDEI CTNAG 5 cut(s) 38, 342, 351, 1374, 2146
BstDSI CCRYGG 2 cut(s) 1505, 2114
BstEII GGTNACC 2 cut(s) 124, 298
BstF5I GGATG 6 cut(s) 832, 1121, 1500, 1678, 1810, 2022
BstHHI GCGC 1 cut(s) 413
BstMAI GTCTC 3 cut(s) 71, 1370, 2149
BstMCI CGRYCG 1 cut(s) 2114
BstMWI GCNNNNNNNGC 7 cut(s) 164, 312, 410, 1262, 1796, 2006, 2228
BstNI CCWGG 5 cut(s) 190, 648, 1080, 1785, 2242
BstNSI RCATGY 1 cut(s) 411
BstPI GGTNACC 2 cut(s) 124, 298
BstSCI CCNGG 8 cut(s) 10, 141, 167, 188, 646, 1078, 1783, 2240
BstSFI CTRYAG 5 cut(s) 368, 816, 882, 1254, 2135
BstSLI GKGCMC 1 cut(s) 590
BstV1I GCAGC 6 cut(s) 354, 382, 497, 912, 1131, 1984
BstV2I GAAGAC 1 cut(s) 315
BstX2I RGATCY 4 cut(s) 20, 433, 878, 2296
BstYI RGATCY 4 cut(s) 20, 433, 878, 2296
BstZ17I GTATAC 1 cut(s) 614
BstZI CGGCCG 1 cut(s) 2111
BsuI GTATCC 2 cut(s) 1296, 1402
BsuRI GGCC 7 cut(s) 188, 404, 915, 1116, 1398, 2113, 2231
BtgI CCRYGG 2 cut(s) 1505, 2114
BtsCI GGATG 6 cut(s) 832, 1121, 1500, 1678, 1810, 2022
BtsI GCAGTG 2 cut(s) 862, 2132
BtsIMutI CAGTG 6 cut(s) 444, 588, 862, 1348, 1938, 2132
BveI ACCTGC 4 cut(s) 752, 1532, 1680, 2128
Cac8I GCNNGC 7 cut(s) 113, 156, 375, 1094, 1476, 1972, 2224
CaiI CAGNNNCTG 2 cut(s) 815, 2000
CciI TCATGA 2 cut(s) 1831, 1888
CfoI GCGC 1 cut(s) 413
Cfr13I GGNCC 4 cut(s) 1115, 1397, 1669, 2229
CseI GACGC 2 cut(s) 488, 2290
CsiI ACCWGGT 1 cut(s) 1783
Csp6I GTAC 6 cut(s) 178, 220, 520, 741, 1002, 1311
CspCI CAANNNNNGTGG 4 cut(s) 229, 264, 2096, 2131
CviQI GTAC 6 cut(s) 178, 220, 520, 741, 1002, 1311
DdeI CTNAG 5 cut(s) 38, 342, 351, 1374, 2146
DraI TTTAAA 3 cut(s) 1636, 1896, 2127
EaeI YGGCCR 1 cut(s) 2111
EagI CGGCCG 1 cut(s) 2111
Eam1104I CTCTTC 3 cut(s) 326, 390, 1301
EarI CTCTTC 3 cut(s) 326, 390, 1301
Ecl136II GAGCTC 2 cut(s) 712, 1474
EclXI CGGCCG 1 cut(s) 2111
Eco130I CCWWGG 1 cut(s) 1505
Eco24I GRGCYC 5 cut(s) 117, 343, 473, 714, 1476
Eco47I GGWCC 1 cut(s) 1669
Eco52I CGGCCG 1 cut(s) 2111
Eco53kI GAGCTC 2 cut(s) 712, 1474
Eco72I CACGTG 1 cut(s) 2201
Eco91I GGTNACC 2 cut(s) 124, 298
EcoICRI GAGCTC 2 cut(s) 712, 1474
EcoO109I RGGNCCY 1 cut(s) 1669
EcoO65I GGTNACC 2 cut(s) 124, 298
EcoRII CCWGG 5 cut(s) 188, 646, 1078, 1783, 2240
EcoT14I CCWWGG 1 cut(s) 1505
EcoT38I GRGCYC 5 cut(s) 117, 343, 473, 714, 1476
ErhI CCWWGG 1 cut(s) 1505
Esp3I CGTCTC 1 cut(s) 71
FaqI GGGAC 3 cut(s) 32, 1058, 2017
FauI CCCGC 1 cut(s) 104
FauNDI CATATG 1 cut(s) 842
FblI GTMKAC 3 cut(s) 613, 922, 1917
FokI GGATG 6 cut(s) 839, 1108, 1487, 1665, 1817, 2029
FriOI GRGCYC 5 cut(s) 117, 343, 473, 714, 1476
FspBI CTAG 4 cut(s) 459, 1455, 2262, 2313
GlaI GCGC 1 cut(s) 412
GsaI CCCAGC 3 cut(s) 340, 2004, 2230
HaeIII GGCC 7 cut(s) 188, 404, 915, 1116, 1398, 2113, 2231
HapII CCGG 5 cut(s) 12, 129, 142, 168, 227
HgaI GACGC 2 cut(s) 488, 2290
HhaI GCGC 1 cut(s) 413
Hin1I GRCGYC 2 cut(s) 480, 2282
Hin6I GCGC 1 cut(s) 411
HinP1I GCGC 1 cut(s) 411
HincII GTYRAC 1 cut(s) 478
HindII GTYRAC 1 cut(s) 478
HindIII AAGCTT 3 cut(s) 32, 782, 1248
HpaII CCGG 5 cut(s) 12, 129, 142, 168, 227
HphI GGTGA 5 cut(s) 272, 292, 514, 1131, 2329
Hpy166II GTNNAC 9 cut(s) 478, 588, 614, 923, 968, 1460, 1767, 1918, 2068
Hpy188I TCNGA 8 cut(s) 39, 400, 597, 774, 867, 1216, 1585, 2062
Hpy188III TCNNGA 4 cut(s) 1373, 1752, 1832, 1889
Hpy8I GTNNAC 9 cut(s) 478, 588, 614, 923, 968, 1460, 1767, 1918, 2068
Hpy99I CGWCG 3 cut(s) 56, 187, 690
HpyAV CCTTC 5 cut(s) 268, 1660, 1930, 1987, 2262
HpyCH4III ACNGT 6 cut(s) 583, 1220, 1558, 1933, 2046, 2072
HpyCH4IV ACGT 4 cut(s) 222, 688, 1564, 2200
HpyF10VI GCNNNNNNNGC 7 cut(s) 164, 312, 410, 1262, 1796, 2006, 2228
HpyF3I CTNAG 5 cut(s) 38, 342, 351, 1374, 2146
HpySE526I ACGT 4 cut(s) 222, 688, 1564, 2200
Hsp92I GRCGYC 2 cut(s) 480, 2282
HspAI GCGC 1 cut(s) 411
LguI GCTCTTC 2 cut(s) 326, 390
LmnI GCTCC 6 cut(s) 131, 287, 476, 709, 717, 1609
Lsp1109I GCAGC 6 cut(s) 354, 382, 497, 912, 1131, 1984
LweI GCATC 2 cut(s) 499, 1687
MabI ACCWGGT 1 cut(s) 1783
MaeI CTAG 4 cut(s) 459, 1455, 2262, 2313
MaeII ACGT 4 cut(s) 222, 688, 1564, 2200
MaeIII GTNAC 8 cut(s) 124, 298, 1082, 1191, 1654, 1708, 1735, 2302
MboII GAAGA 7 cut(s) 315, 343, 377, 1128, 1318, 1637, 1850
MfeI CAATTG 2 cut(s) 621, 960
MflI RGATCY 4 cut(s) 20, 433, 878, 2296
MhlI GDGCHC 7 cut(s) 117, 343, 444, 473, 590, 714, 1476
MlyI GAGTC 2 cut(s) 1415, 2299
MmeI TCCRAC 1 cut(s) 48
MseI TTAA 7 cut(s) 632, 677, 728, 1230, 1635, 1895, 2126
MslI CAYNNNNRTG 2 cut(s) 752, 1392
MspA1I CMGCKG 7 cut(s) 111, 306, 367, 373, 1144, 1997, 2150
MspI CCGG 5 cut(s) 12, 129, 142, 168, 227
MspR9I CCNGG 8 cut(s) 12, 143, 169, 190, 648, 1080, 1785, 2242
MunI CAATTG 2 cut(s) 621, 960
MvaI CCWGG 5 cut(s) 190, 648, 1080, 1785, 2242
MwoI GCNNNNNNNGC 7 cut(s) 164, 312, 410, 1262, 1796, 2006, 2228
NciI CCSGG 3 cut(s) 12, 143, 169
NcoI CCATGG 1 cut(s) 1505
NdeI CATATG 1 cut(s) 842
NlaIV GGNNCC 6 cut(s) 133, 173, 435, 472, 1452, 2022
NmuCI GTSAC 3 cut(s) 298, 1735, 2302
NspI RCATGY 1 cut(s) 411
NspV TTCGAA 1 cut(s) 268
PagI TCATGA 2 cut(s) 1831, 1888
PciSI GCTCTTC 2 cut(s) 326, 390
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 8 cut(s) 148, 160, 449, 626, 1100, 1600, 2194, 2273
PleI GAGTC 2 cut(s) 1415, 2299
PmaCI CACGTG 1 cut(s) 2201
PmlI CACGTG 1 cut(s) 2201
PpsI GAGTC 2 cut(s) 1415, 2299
Ppu21I YACGTR 2 cut(s) 223, 2201
PpuMI RGGWCCY 1 cut(s) 1669
PsiI TTATAA 1 cut(s) 2163
Psp124BI GAGCTC 2 cut(s) 714, 1476
Psp5II RGGWCCY 1 cut(s) 1669
Psp6I CCWGG 5 cut(s) 188, 646, 1078, 1783, 2240
PspCI CACGTG 1 cut(s) 2201
PspEI GGTNACC 2 cut(s) 124, 298
PspFI CCCAGC 3 cut(s) 336, 2000, 2226
PspGI CCWGG 5 cut(s) 188, 646, 1078, 1783, 2240
PspN4I GGNNCC 6 cut(s) 133, 173, 435, 472, 1452, 2022
PspPI GGNCC 4 cut(s) 1115, 1397, 1669, 2229
PspPPI RGGWCCY 1 cut(s) 1669
PstI CTGCAG 3 cut(s) 372, 1258, 2139
PstNI CAGNNNCTG 2 cut(s) 815, 2000
PsuI RGATCY 4 cut(s) 20, 433, 878, 2296
PvuII CAGCTG 4 cut(s) 367, 373, 1997, 2150
RsaI GTAC 6 cut(s) 179, 221, 521, 742, 1003, 1312
RsaNI GTAC 6 cut(s) 178, 220, 520, 741, 1002, 1311
RseI CAYNNNNRTG 2 cut(s) 752, 1392
SacI GAGCTC 2 cut(s) 714, 1476
SapI GCTCTTC 2 cut(s) 326, 390
SaqAI TTAA 7 cut(s) 632, 677, 728, 1230, 1635, 1895, 2126
Sau96I GGNCC 4 cut(s) 1115, 1397, 1669, 2229
SchI GAGTC 2 cut(s) 1415, 2299
ScrFI CCNGG 8 cut(s) 12, 143, 169, 190, 648, 1080, 1785, 2242
SduI GDGCHC 7 cut(s) 117, 343, 444, 473, 590, 714, 1476
SexAI ACCWGGT 1 cut(s) 1783
SfaNI GCATC 2 cut(s) 499, 1687
SfcI CTRYAG 5 cut(s) 368, 816, 882, 1254, 2135
SfuI TTCGAA 1 cut(s) 268
SinI GGWCC 1 cut(s) 1669
SmiI ATTTAAAT 1 cut(s) 2127
SmiMI CAYNNNNRTG 2 cut(s) 752, 1392
SmlI CTYRAG 1 cut(s) 1423
SmoI CTYRAG 1 cut(s) 1423
SspI AATATT 3 cut(s) 834, 1745, 2085
SspMI CTAG 4 cut(s) 459, 1455, 2262, 2313
SstI GAGCTC 2 cut(s) 714, 1476
StyD4I CCNGG 8 cut(s) 10, 141, 167, 188, 646, 1078, 1783, 2240
StyI CCWWGG 1 cut(s) 1505
SwaI ATTTAAAT 1 cut(s) 2127
TaaI ACNGT 6 cut(s) 583, 1220, 1558, 1933, 2046, 2072
TaiI ACGT 4 cut(s) 225, 691, 1567, 2203
TaqI TCGA 8 cut(s) 117, 206, 268, 444, 685, 976, 1062, 1444
TatI WGTACW 2 cut(s) 1001, 1310
TauI GCSGC 7 cut(s) 68, 315, 318, 1678, 1720, 1818, 2210
TfiI GAWTC 8 cut(s) 148, 160, 449, 626, 1100, 1600, 2194, 2273
Tru1I TTAA 7 cut(s) 632, 677, 728, 1230, 1635, 1895, 2126
Tru9I TTAA 7 cut(s) 632, 677, 728, 1230, 1635, 1895, 2126
TscAI CASTG 6 cut(s) 444, 595, 862, 1348, 1938, 2139
TseFI GTSAC 3 cut(s) 298, 1735, 2302
TseI GCWGC 6 cut(s) 367, 370, 510, 900, 1144, 1997
Tsp45I GTSAC 3 cut(s) 298, 1735, 2302
TspGWI ACGGA 3 cut(s) 95, 463, 1754
TspRI CASTG 6 cut(s) 444, 595, 862, 1348, 1938, 2139
VneI GTGCAC 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 1669
XapI RAATTY 4 cut(s) 263, 1368, 1492, 1640
XceI RCATGY 1 cut(s) 411
XmiI GTMKAC 3 cut(s) 613, 922, 1917
XspI CTAG 4 cut(s) 459, 1455, 2262, 2313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.