MD12G1032300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013091
Physical Location & Seq
Forward (+)
3562193 .. 3565381
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1032300.v1.1.491

Sequence Viewer

Length: 2040 bp
ATGCCGAGGCAGAACAGCAATTTGGCGATGGACCTGATGATCCCCGGAAAAATCCGGAAGCGGGGGTGTTCGTCGTCGGCGTCTTCGTCGTCTTCGATCATCCAGAACTACAGATTCAAGCGGGCGATACTCGTTGGGAAGAGGGGCGGATCCAGTACGCCCGTGCCCACGTGGAAGCTCATGAGCTCCAGGTCCCCGACGGCTTCCGCATTGCGCGCCATGGACTCCCCCAACTACTCCCAGAGCGGCGGCGCCAGGTCCAAGCAGCAGCAGCAGCAACCCGTGTCGGCGAGGAAACTCGCTGCCACGCTCTGGGAAATGAACGACATGTCTCCCAGAGTGAAGGAAGGGTCGGACGAGAGGCGGCTGAGGAAGATGGAGGCGAGTAATCGTAAAGCCAGAGAGAGGGAGAGGATCCCGAGATCGGCGCACTCCGGTTCTCTGCCGCCCCATCTCTCCGATCCGTCTCATAGTCCCGTTTCCGAGAGGATGGATCGGTCTGGAACGGGTAGTTTTCATAGAAGGGCGTCGCCCATTTCGCAGAGGCGGCTCAGGATCACAGACCACCATTCTGGAATGTTGGATTCTCATGGCAATGGCAGTTTGATGGAGGTTGAGACTAGATCTCGAGCCCAGACGCCTACTGCTTCTACTGCTGGAGCCAAGACACGTTTGAAGGATGTCAGTAATGCTTTGACCACGTCTAAAGAGCTACTGAAAATTATCAACCGTGTTTGGGGTAATGAAGATCGACCTTCAACAAGCGTGGCCCTTATCTCAGCCTTGCATGCTGAGCTGGAGAGGGCTCGTTTACACGTCAATCAGCTTATCCAAGAACAACGCTCAGACCAGAATGAGATCAACTACCTCATGAAGTGTTTTGCTGAAGAAAAGGCAGCTTGGAAAAGCAAGGAGCATAAAGTTGTTGAGGCTGCCATCGAGGCCGTTGCTGGAGAACTTGAGGTAGAGAGGAAACTGAGGAGGAGGTTCGAAAGCCTGAACAAGAAACTCGGGAAAGAACTGGCGGAGACAAAAGCCTCTCTTGTTAATAAGGCAAAAGAACTCGAAAGTGAGAAGAGAACAAGAGAGATCATGGAACAAGTATGTGATGAATTAGCCAGGGATATGACTGAAGATAAAGATGAAGCAGAGGAAATGAAGAGAGAATCTGCAAAAGTTCGTGATGAGGTTGAGAAGGAAAGGGAGATGATGCAGTTTGCTGACGTGTTGCGTGAGGAGAGAGCTCAAGTGAAACTCTCTGAGGCAAAACATCATCTTGAAGAGAAGAATGCAGCTGTTGATATGTTGAGGAGTCAACTTGAAGCCTTCATGGGAAGTAAGACAACCAAAGAAAAAGGACGCACTTCTACTCATCTGAATGATGAAGAAATTTCTGCATATCTAAGTAGAGCTCATCTTGGTTCCGGTCAGGATGAAGAAAAAGATGAGGATGAAGGAGAAGTTGAAGACGGGGTAGAATGCGAAGAGGGTTCTGCTGAAAGTGATCTTCATTCCATAGAGTTAAGTATGGGCAACAACAGCAAAAGCTACAACTTGAATCACAATTCTGCAGCCACTCGTGATCCAAGGTGGGCAGCACCTGGTGTCGAAGAAATCAAAGGGAGGAAGTCTACTTCTGGGAAGCCACCAAGAAGAAGCACTTCTTTGCAAAGAAGCATATCAGATGGAGTGGAATGGGGCATGCAAACTGAAAGGCTCCAAAATTCAGGAGACGGGATAGATTGGGAGAGGTTTCCTGAACTGGAGAGGCAAAGGCAACGGCAAGGAAAAGGATGTGCAGAGGAAATGCAAGGATATAAATCATCAAAGGGTCTTAGGGACCAAATGTTGTCTGGTTCAAGGCTTGGACCTGCTAGAGTTCACGCTAGTCCCACACGGCAATGGGGGCAGCCGTGGCCTTCACGGGATCCTACCAACACATTCCAAGATAGGCCTCCCAGCGCGCAAGGAAGTGGTTCAAAGTCAAACTTGGCGGAAGTGAGAGGTGAAGGCCAGAATGGCAGAAGGTATAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

680

Amino Acids

76.26

Weight (kDa)

9.33

Isoelectric Point (pI)

54.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11590 AT3G11590
fragaria_vesca FvH4_6g19640
malus_domestica MD12G1032300.v1.1 MD14G1032400.v1.1
prunus_persica Prupe.7G100300_v2.0.a1
pyrus_communis pycom12g02990 pycom14g02920
rosa_chinensis RchiOBHm_Chr3g0474981
rosa_laevigata RLG00000023874
rosa_multiflora Rmu_sc0003093.1_g000007
rosa_roxburghii Rroxscaffold_6G00406670
rosa_rugosa Rorug03G0145900
rosa_samantha Rh3AG195200 Rh3BG225300 Rh3CG220200 Rh3DG220700
rosa_wichuraiana Rw3G017780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1879
AccB1I GGYRCC 1 cut(s) 251
AccB7I CCANNNNNTGG 1 cut(s) 312
AccBSI CCGCTC 1 cut(s) 246
AccI GTMKAC 1 cut(s) 1631
AccII CGCG 2 cut(s) 216, 1964
AccIII TCCGGA 1 cut(s) 54
AcsI RAATTY 2 cut(s) 1389, 1723
AcuI CTGAAG 2 cut(s) 906, 1152
AcvI CACGTG 1 cut(s) 171
AcyI GRCGYC 4 cut(s) 80, 252, 527, 638
AdeI CACNNNGTG 1 cut(s) 1604
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 5 cut(s) 61, 312, 405, 424, 736
AflIII ACRYGT 4 cut(s) 327, 668, 814, 1224
AgsI TTSAA 9 cut(s) 118, 676, 759, 1280, 1322, 1466, 1558, 1860, 1980
AjiI CACGTC 3 cut(s) 702, 817, 1225
AjnI CCWGG 4 cut(s) 188, 254, 1118, 1600
AjuI GAANNNNNNNTTGG 1 cut(s) 37
Alw21I GWGCWC 3 cut(s) 188, 1246, 1414
Alw26I GTCTC 5 cut(s) 336, 471, 611, 1022, 1725
AlwNI CAGNNNCTG 1 cut(s) 1601
Ama87I CYCGRG 3 cut(s) 418, 627, 1010
Aor13HI TCCGGA 1 cut(s) 54
AoxI GGCC 5 cut(s) 768, 942, 1916, 1952, 2011
ApoI RAATTY 2 cut(s) 1389, 1723
Asp700I GAANNNNTTC 2 cut(s) 1660, 1975
AspLEI GCGC 6 cut(s) 216, 218, 254, 430, 1964, 1966
AspS9I GGNCC 6 cut(s) 31, 192, 258, 769, 1840, 1868
AsuC2I CCSGG 1 cut(s) 45
AsuHPI GGTGA 1 cut(s) 2018
AsuII TTCGAA 1 cut(s) 990
AvaI CYCGRG 3 cut(s) 418, 627, 1010
AvaII GGWCC 5 cut(s) 31, 192, 258, 1840, 1868
BaeGI GKGCMC 1 cut(s) 168
BamHI GGATCC 3 cut(s) 149, 414, 1927
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 5 cut(s) 188, 634, 808, 1246, 1414
BauI CACGAG 1 cut(s) 1578
BbrPI CACGTG 1 cut(s) 171
BbsI GAAGAC 3 cut(s) 75, 84, 1473
Bbv12I GWGCWC 3 cut(s) 188, 1246, 1414
BbvCI CCTCAGC 1 cut(s) 368
BccI CCATC 7 cut(s) 22, 370, 459, 484, 601, 944, 1679
BceAI ACGGC 5 cut(s) 216, 929, 1796, 1897, 1913
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 4 cut(s) 190, 256, 1120, 1602
BcnI CCSGG 1 cut(s) 45
BcoDI GTCTC 5 cut(s) 336, 471, 611, 1022, 1725
BfaI CTAG 3 cut(s) 621, 1875, 1887
BfmI CTRYAG 2 cut(s) 109, 1569
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 1879
BglI GCCNNNNNGGC 2 cut(s) 941, 2019
BglII AGATCT 1 cut(s) 623
BlpI GCTNAGC 1 cut(s) 792
Bme1390I CCNGG 5 cut(s) 45, 190, 256, 1120, 1602
Bme18I GGWCC 5 cut(s) 31, 192, 258, 1840, 1868
BmeT110I CYCGRG 3 cut(s) 418, 627, 1010
BmgBI CACGTC 3 cut(s) 702, 817, 1225
BmgT120I GGNCC 6 cut(s) 31, 192, 258, 769, 1840, 1868
BmiI GGNNCC 9 cut(s) 151, 194, 253, 416, 661, 1423, 1718, 1841, 1929
BmrFI CCNGG 5 cut(s) 45, 190, 256, 1120, 1602
BmsI GCATC 1 cut(s) 1200
BpiI GAAGAC 3 cut(s) 75, 84, 1473
BpmI CTGGAG 5 cut(s) 172, 678, 818, 972, 1784
Bpu10I CCTNAGC 2 cut(s) 368, 551
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 990
BpuEI CTTGAG 2 cut(s) 980, 1230
BpuMI CCSGG 1 cut(s) 45
BsaAI YACGTR 1 cut(s) 171
BsaBI GATNNNNATC 1 cut(s) 1819
BsaHI GRCGYC 4 cut(s) 80, 252, 527, 638
BsaJI CCNNGG 6 cut(s) 5, 43, 219, 1119, 1586, 1913
BsaWI WCCGGW 3 cut(s) 54, 434, 1424
Bsc4I CCNNNNNNNGG 5 cut(s) 61, 312, 405, 424, 736
Bse1I ACTGG 3 cut(s) 153, 1026, 1767
Bse3DI GCAATG 3 cut(s) 209, 601, 1907
Bse8I GATNNNNATC 1 cut(s) 1819
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 4 cut(s) 190, 256, 1120, 1602
BseDI CCNNGG 6 cut(s) 5, 43, 219, 1119, 1586, 1913
BseGI GGATG 6 cut(s) 99, 495, 685, 1438, 1456, 1799
BseJI GATNNNNATC 1 cut(s) 1819
BseLI CCNNNNNNNGG 5 cut(s) 61, 312, 405, 424, 736
BseMI GCAATG 3 cut(s) 209, 601, 1907
BseMII CTCAG 7 cut(s) 359, 565, 783, 792, 858, 968, 1251
BseNI ACTGG 3 cut(s) 153, 1026, 1767
BsePI GCGCGC 2 cut(s) 214, 1962
BseRI GAGGAG 4 cut(s) 994, 997, 1250, 1324
BseSI GKGCMC 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 1958
BsgI GTGCAG 1 cut(s) 1818
Bsh1236I CGCG 2 cut(s) 216, 1964
BshFI GGCC 5 cut(s) 770, 944, 1918, 1954, 2013
BshNI GGYRCC 1 cut(s) 251
BsiHKAI GWGCWC 3 cut(s) 188, 1246, 1414
BsiHKCI CYCGRG 3 cut(s) 418, 627, 1010
BsiSI CCGG 4 cut(s) 45, 55, 435, 1425
BslFI GGGAC 4 cut(s) 178, 459, 1853, 1875
BslI CCNNNNNNNGG 5 cut(s) 61, 312, 405, 424, 736
BsmAI GTCTC 5 cut(s) 336, 471, 611, 1022, 1725
BsmBI CGTCTC 2 cut(s) 471, 1725
BsmFI GGGAC 4 cut(s) 178, 459, 1853, 1875
BsmI GAATGC 2 cut(s) 1294, 1484
BsnI GGCC 5 cut(s) 770, 944, 1918, 1954, 2013
BsoBI CYCGRG 3 cut(s) 418, 627, 1010
Bsp119I TTCGAA 1 cut(s) 990
Bsp1286I GDGCHC 6 cut(s) 168, 188, 634, 808, 1246, 1414
Bsp13I TCCGGA 1 cut(s) 54
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp19I CCATGG 1 cut(s) 219
BspANI GGCC 5 cut(s) 770, 944, 1918, 1954, 2013
BspCNI CTCAG 7 cut(s) 360, 564, 784, 791, 857, 969, 1252
BspEI TCCGGA 1 cut(s) 54
BspFNI CGCG 2 cut(s) 216, 1964
BspHI TCATGA 2 cut(s) 180, 870
BspLI GGNNCC 9 cut(s) 151, 194, 253, 416, 661, 1423, 1718, 1841, 1929
BspMAI CTGCAG 1 cut(s) 1573
BspMI ACCTGC 1 cut(s) 1879
BspT104I TTCGAA 1 cut(s) 990
BspT107I GGYRCC 1 cut(s) 251
BsrBI CCGCTC 1 cut(s) 246
BsrDI GCAATG 3 cut(s) 209, 601, 1907
BsrI ACTGG 3 cut(s) 153, 1026, 1767
BssECI CCNNGG 6 cut(s) 5, 43, 219, 1119, 1586, 1913
BssHII GCGCGC 2 cut(s) 214, 1962
BssNI GRCGYC 4 cut(s) 80, 252, 527, 638
BssSI CACGAG 1 cut(s) 1578
BssT1I CCWWGG 2 cut(s) 219, 1586
Bst2BI CACGAG 1 cut(s) 1578
Bst2UI CCWGG 4 cut(s) 190, 256, 1120, 1602
Bst4CI ACNGT 2 cut(s) 731, 2037
Bst6I CTCTTC 5 cut(s) 134, 1070, 1154, 1275, 1479
BstACI GRCGYC 4 cut(s) 80, 252, 527, 638
BstBAI YACGTR 1 cut(s) 171
BstBI TTCGAA 1 cut(s) 990
BstC8I GCNNGC 5 cut(s) 123, 216, 789, 1703, 1964
BstDEI CTNAG 9 cut(s) 368, 551, 778, 792, 844, 977, 1260, 1403, 1835
BstDSI CCRYGG 2 cut(s) 219, 1913
BstF5I GGATG 6 cut(s) 99, 495, 685, 1438, 1456, 1799
BstFNI CGCG 2 cut(s) 216, 1964
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 6 cut(s) 216, 218, 254, 430, 1964, 1966
BstMAI GTCTC 5 cut(s) 336, 471, 611, 1022, 1725
BstNI CCWGG 4 cut(s) 190, 256, 1120, 1602
BstNSI RCATGY 3 cut(s) 331, 791, 1705
BstSCI CCNGG 5 cut(s) 43, 188, 254, 1118, 1600
BstSFI CTRYAG 2 cut(s) 109, 1569
BstSLI GKGCMC 1 cut(s) 168
BstUI CGCG 2 cut(s) 216, 1964
BstV2I GAAGAC 3 cut(s) 75, 84, 1473
BstX2I RGATCY 4 cut(s) 149, 414, 623, 1927
BstXI CCANNNNNNTGG 1 cut(s) 572
BstYI RGATCY 4 cut(s) 149, 414, 623, 1927
BsuRI GGCC 5 cut(s) 770, 944, 1918, 1954, 2013
BtgI CCRYGG 2 cut(s) 219, 1913
BtgZI GCGATG 1 cut(s) 41
BtrI CACGTC 3 cut(s) 702, 817, 1225
BtsCI GGATG 6 cut(s) 99, 495, 685, 1438, 1456, 1799
BveI ACCTGC 1 cut(s) 1879
Cac8I GCNNGC 5 cut(s) 123, 216, 789, 1703, 1964
CaiI CAGNNNCTG 1 cut(s) 1601
CciI TCATGA 2 cut(s) 180, 870
CfoI GCGC 6 cut(s) 216, 218, 254, 430, 1964, 1966
Cfr13I GGNCC 6 cut(s) 31, 192, 258, 769, 1840, 1868
CseI GACGC 4 cut(s) 69, 516, 646, 1368
CsiI ACCWGGT 1 cut(s) 1600
Csp6I GTAC 1 cut(s) 156
CspCI CAANNNNNGTGG 2 cut(s) 747, 782
CviAII CATG 9 cut(s) 181, 220, 328, 590, 788, 871, 1093, 1330, 1702
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 9 cut(s) 368, 551, 778, 792, 844, 977, 1260, 1403, 1835
DinI GGCGCC 1 cut(s) 253
DraIII CACNNNGTG 1 cut(s) 1604
Eam1104I CTCTTC 5 cut(s) 134, 1070, 1154, 1275, 1479
EarI CTCTTC 5 cut(s) 134, 1070, 1154, 1275, 1479
EciI GGCGGA 3 cut(s) 162, 1040, 2009
Ecl136II GAGCTC 3 cut(s) 186, 1244, 1412
Eco130I CCWWGG 2 cut(s) 219, 1586
Eco147I AGGCCT 1 cut(s) 1954
Eco24I GRGCYC 5 cut(s) 188, 634, 808, 1246, 1414
Eco47I GGWCC 5 cut(s) 31, 192, 258, 1840, 1868
Eco53kI GAGCTC 3 cut(s) 186, 1244, 1412
Eco57I CTGAAG 2 cut(s) 906, 1152
Eco72I CACGTG 1 cut(s) 171
Eco88I CYCGRG 3 cut(s) 418, 627, 1010
EcoICRI GAGCTC 3 cut(s) 186, 1244, 1412
EcoO109I RGGNCCY 1 cut(s) 192
EcoRII CCWGG 4 cut(s) 188, 254, 1118, 1600
EcoT14I CCWWGG 2 cut(s) 219, 1586
EcoT38I GRGCYC 5 cut(s) 188, 634, 808, 1246, 1414
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 2 cut(s) 219, 1586
Esp3I CGTCTC 2 cut(s) 471, 1725
FaeI CATG 9 cut(s) 184, 223, 331, 593, 791, 874, 1096, 1333, 1705
FalI AAGNNNNNCTT 8 cut(s) 1026, 1058, 1645, 1677, 1648, 1680, 1973, 2005
FaqI GGGAC 4 cut(s) 178, 459, 1853, 1875
FatI CATG 9 cut(s) 180, 219, 327, 589, 787, 870, 1092, 1329, 1701
FauI CCCGC 2 cut(s) 54, 114
FblI GTMKAC 1 cut(s) 1631
FokI GGATG 6 cut(s) 86, 502, 692, 1445, 1463, 1806
FriOI GRGCYC 5 cut(s) 188, 634, 808, 1246, 1414
FspBI CTAG 3 cut(s) 621, 1875, 1887
GlaI GCGC 6 cut(s) 215, 217, 253, 429, 1963, 1965
GsaI CCCAGC 1 cut(s) 1962
GsuI CTGGAG 5 cut(s) 172, 678, 818, 972, 1784
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 5 cut(s) 770, 944, 1918, 1954, 2013
HapII CCGG 4 cut(s) 45, 55, 435, 1425
HgaI GACGC 4 cut(s) 69, 516, 646, 1368
HhaI GCGC 6 cut(s) 216, 218, 254, 430, 1964, 1966
Hin1I GRCGYC 4 cut(s) 80, 252, 527, 638
Hin1II CATG 9 cut(s) 184, 223, 331, 593, 791, 874, 1096, 1333, 1705
Hin6I GCGC 6 cut(s) 214, 216, 252, 428, 1962, 1964
HinP1I GCGC 6 cut(s) 214, 216, 252, 428, 1962, 1964
HincII GTYRAC 1 cut(s) 1316
HindII GTYRAC 1 cut(s) 1316
HinfI GANTC 6 cut(s) 114, 224, 584, 1166, 1312, 1558
HpaII CCGG 4 cut(s) 45, 55, 435, 1425
HphI GGTGA 1 cut(s) 2018
Hpy166II GTNNAC 4 cut(s) 812, 1316, 1632, 1882
Hpy188I TCNGA 7 cut(s) 355, 460, 484, 847, 1261, 1377, 1684
Hpy8I GTNNAC 4 cut(s) 812, 1316, 1632, 1882
Hpy99I CGWCG 5 cut(s) 76, 79, 91, 202, 532
HpyCH4III ACNGT 2 cut(s) 731, 2037
HpyCH4IV ACGT 5 cut(s) 170, 670, 701, 816, 1224
HpyF3I CTNAG 9 cut(s) 368, 551, 778, 792, 844, 977, 1260, 1403, 1835
HpySE526I ACGT 5 cut(s) 170, 670, 701, 816, 1224
Hsp92I GRCGYC 4 cut(s) 80, 252, 527, 638
Hsp92II CATG 9 cut(s) 184, 223, 331, 593, 791, 874, 1096, 1333, 1705
HspAI GCGC 6 cut(s) 214, 216, 252, 428, 1962, 1964
KasI GGCGCC 1 cut(s) 251
Kpn2I TCCGGA 1 cut(s) 54
LmnI GCTCC 4 cut(s) 191, 659, 913, 1722
LweI GCATC 1 cut(s) 1200
MabI ACCWGGT 1 cut(s) 1600
MaeI CTAG 3 cut(s) 621, 1875, 1887
MaeII ACGT 5 cut(s) 170, 670, 701, 816, 1224
MbiI CCGCTC 1 cut(s) 246
MflI RGATCY 4 cut(s) 149, 414, 623, 1927
MhlI GDGCHC 6 cut(s) 168, 188, 634, 808, 1246, 1414
MluCI AATT 6 cut(s) 19, 720, 1112, 1389, 1564, 1723
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 218, 1321
MmeI TCCRAC 2 cut(s) 333, 561
MroI TCCGGA 1 cut(s) 54
MroXI GAANNNNTTC 2 cut(s) 1660, 1975
MseI TTAA 2 cut(s) 1047, 1523
MslI CAYNNNNRTG 3 cut(s) 594, 1377, 1900
MspA1I CMGCKG 1 cut(s) 1295
MspI CCGG 4 cut(s) 45, 55, 435, 1425
MspR9I CCNGG 5 cut(s) 45, 190, 256, 1120, 1602
Mva1269I GAATGC 2 cut(s) 1294, 1484
MvaI CCWGG 4 cut(s) 190, 256, 1120, 1602
MvnI CGCG 2 cut(s) 216, 1964
NarI GGCGCC 1 cut(s) 252
NciI CCSGG 1 cut(s) 45
NcoI CCATGG 1 cut(s) 219
NlaIII CATG 9 cut(s) 184, 223, 331, 593, 791, 874, 1096, 1333, 1705
NlaIV GGNNCC 9 cut(s) 151, 194, 253, 416, 661, 1423, 1718, 1841, 1929
NmeAIII GCCGAG 1 cut(s) 30
NspI RCATGY 3 cut(s) 331, 791, 1705
NspV TTCGAA 1 cut(s) 990
PaeI GCATGC 2 cut(s) 791, 1705
PaeR7I CTCGAG 1 cut(s) 627
PagI TCATGA 2 cut(s) 180, 870
PauI GCGCGC 2 cut(s) 214, 1962
PceI AGGCCT 1 cut(s) 1954
PciI ACATGT 1 cut(s) 327
PcsI WCGNNNNNNNCGW 3 cut(s) 77, 83, 92
PctI GAATGC 2 cut(s) 1294, 1484
PdmI GAANNNNTTC 2 cut(s) 1660, 1975
PfeI GAWTC 4 cut(s) 114, 584, 1166, 1558
PflFI GACNNNGTC 1 cut(s) 700
PflMI CCANNNNNTGG 1 cut(s) 312
PleI GAGTC 2 cut(s) 218, 1320
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 171
PmlI CACGTG 1 cut(s) 171
PpsI GAGTC 2 cut(s) 218, 1320
Ppu21I YACGTR 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 192
PscI ACATGT 1 cut(s) 327
Psp124BI GAGCTC 3 cut(s) 188, 1246, 1414
Psp5II RGGWCCY 1 cut(s) 192
Psp6I CCWGG 4 cut(s) 188, 254, 1118, 1600
PspCI CACGTG 1 cut(s) 171
PspFI CCCAGC 1 cut(s) 1958
PspGI CCWGG 4 cut(s) 188, 254, 1118, 1600
PspN4I GGNNCC 9 cut(s) 151, 194, 253, 416, 661, 1423, 1718, 1841, 1929
PspPI GGNCC 6 cut(s) 31, 192, 258, 769, 1840, 1868
PspPPI RGGWCCY 1 cut(s) 192
PstI CTGCAG 1 cut(s) 1573
PstNI CAGNNNCTG 1 cut(s) 1601
PsuI RGATCY 4 cut(s) 149, 414, 623, 1927
PsyI GACNNNGTC 1 cut(s) 700
PteI GCGCGC 2 cut(s) 214, 1962
PvuII CAGCTG 1 cut(s) 1295
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
RseI CAYNNNNRTG 3 cut(s) 594, 1377, 1900
SacI GAGCTC 3 cut(s) 188, 1246, 1414
SaqAI TTAA 2 cut(s) 1047, 1523
Sau96I GGNCC 6 cut(s) 31, 192, 258, 769, 1840, 1868
SchI GAGTC 2 cut(s) 218, 1321
ScrFI CCNGG 5 cut(s) 45, 190, 256, 1120, 1602
SduI GDGCHC 6 cut(s) 168, 188, 634, 808, 1246, 1414
SexAI ACCWGGT 1 cut(s) 1600
SfaNI GCATC 1 cut(s) 1200
SfcI CTRYAG 2 cut(s) 109, 1569
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 627
SfuI TTCGAA 1 cut(s) 990
SinI GGWCC 5 cut(s) 31, 192, 258, 1840, 1868
SlaI CTCGAG 1 cut(s) 627
SmiMI CAYNNNNRTG 3 cut(s) 594, 1377, 1900
SmlI CTYRAG 3 cut(s) 627, 959, 1245
SmoI CTYRAG 3 cut(s) 627, 959, 1245
SphI GCATGC 2 cut(s) 791, 1705
Sse9I AATT 6 cut(s) 19, 720, 1112, 1389, 1564, 1723
SseBI AGGCCT 1 cut(s) 1954
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 3 cut(s) 621, 1875, 1887
SstI GAGCTC 3 cut(s) 188, 1246, 1414
StuI AGGCCT 1 cut(s) 1954
StyD4I CCNGG 5 cut(s) 43, 188, 254, 1118, 1600
StyI CCWWGG 2 cut(s) 219, 1586
TaaI ACNGT 2 cut(s) 731, 2037
TaiI ACGT 5 cut(s) 173, 673, 704, 819, 1227
TaqI TCGA 7 cut(s) 95, 628, 751, 939, 990, 1065, 1608
TaqII GACCGA 1 cut(s) 486
TasI AATT 6 cut(s) 19, 720, 1112, 1389, 1564, 1723
TauI GCSGC 5 cut(s) 249, 252, 367, 448, 550
TfiI GAWTC 4 cut(s) 114, 584, 1166, 1558
Tru1I TTAA 2 cut(s) 1047, 1523
Tru9I TTAA 2 cut(s) 1047, 1523
TspGWI ACGGA 1 cut(s) 453
Tth111I GACNNNGTC 1 cut(s) 700
Van91I CCANNNNNTGG 1 cut(s) 312
VpaK11BI GGWCC 5 cut(s) 31, 192, 258, 1840, 1868
XapI RAATTY 2 cut(s) 1389, 1723
XceI RCATGY 3 cut(s) 331, 791, 1705
XcmI CCANNNNNNNNNTGG 1 cut(s) 1850
XhoI CTCGAG 1 cut(s) 627
XmiI GTMKAC 1 cut(s) 1631
XmnI GAANNNNTTC 2 cut(s) 1660, 1975
XspI CTAG 3 cut(s) 621, 1875, 1887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.