Rh3DG220700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0013091
Physical Location & Seq
Forward (+)
20679490 .. 20683412
3923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG220700.1

Sequence Viewer

Length: 1566 bp
ATGGATCGATCTGGAACAGGTAGTTTGCACAGAAGGTCATCCTCTATTTCACAGAGGCTCAGGCTTACAGATCAACATTCTGGAATATTGGATTCTCATGGAAATGGCAGCCTAATGGAGGTTGAGTCTCGATCAAGAGCCCAGACTCCGACTAGATCGAGAGCTCAGACTCCTAGTGCTTCAACTGTTGGAATCAAGCCACGTTTGAAGGATGTTAGTAATGCTCTGACAACGTCTAAAGAGCTACTGAAAATTATACACCGCATTTGGGGTAATGAAGATCAGCCTTCATCTAGCATGGCCCTTATCTCAGCCTTACATGCTGAGCTCGAGAGGGCTCGTTTGCTGGTGAATCAGCATATCCACGAACAGCGCTCAGAGCAGAATGAGATCAGCTATCTCATGAAGTGTTTTGCTGAAGAAAAGGCAGCTTGGAAAAGCAAGGAGCACAAAGTAGTTGAGGCTGCCATCGAGTCTGTTGCAGAAGAACTTGAGGTGGAGAGAAAACTGAGAAGGAGGCTTGAAAGCTTGAACAAGAAACTCGGGAAAGAACTGGCAGAGACAAAAGCATCTCTCGTTAAGGCAGTCAAAGAACTTGAGAGTGAGAAGAGAACGAGAGAGATCATGGAACAAGTGTGTGATGAATTAGCCAGGGATATTAATGAAGATAAACTTGAAGCAGAGGAACTGAAGTGGGAGTCTGCAAAAGTTCATGAAGAGGTCGAAAAGGAAAGGGAGATGATGCAGTTCTCTGATGTGCTGCGTGAGGGGAGAGCTCAAGAGAAACTCTCAGAGGCAAAATATCAGCTTGAGGAGAAGAATGCTGCTGTTGATATGTTGAGGAGTCAACTTGAAGCCTTTATGGGAAGTAAGAGAACAAAAGAAAAAGGACGCGGTTCTGCTCATCTGAAAGATGAAGATATTGCAGCCTATCTAAGTAGAACTTGCCTTGGTTCCCATCAGGGTGAAGAAAAAGATGAGGATGGAGGAGAAGTTGAAGATGGAGTAGAATGTGAAGAGGATTCTGCTGAAAGTGATTTGCATTCCATAGAATTAAGTATGGGCAACAATAACAAAAGCTATAACTGGAGTCATGCTTCTGTAGCTGCTCGGGATCCAAGATGGGCTCCAAATGATGACGAAGAAATCAAAGGGAGGAAGTCCATGTCAGGGAAGCCACCAAGAAAAAGCACTTCTTTGCAAAGGAGTATATCAGATGGATTGGAATGGGGCATGCAGACTGAAAGGCTCCAAAATTCAGGAGATAGGGTAGATTGGGAGAGCTTTCCCGACCTGGAGAGGCAAATGCAAGGAAAGGGTTGTGGAGACGAAATGCAAGGGTATAAATCATCGAAGGGTCTTAGGGACCAAATGTTATCTGGCTCTAGGTTTGGATCTGCTAGAGTTCATGCTAGTCCAACACGGCAGTGGGGGCAGCCGTGGCCTTCACGAGATCCTACGAGTGCAGTTCACGATAGGCCTCCATCTGTCCATGGCAGTGGTTCAAAGTCAAGACTAGCAGAACTAAGAGGTGAAGGCCAGAATGGCAGAAGGTCTAAACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

521

Amino Acids

58.75

Weight (kDa)

6.3

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013091)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11590 AT3G11590
fragaria_vesca FvH4_6g19640
malus_domestica MD12G1032300.v1.1 MD14G1032400.v1.1
prunus_persica Prupe.7G100300_v2.0.a1
pyrus_communis pycom12g02990 pycom14g02920
rosa_chinensis RchiOBHm_Chr3g0474981
rosa_laevigata RLG00000023874
rosa_multiflora Rmu_sc0003093.1_g000007
rosa_roxburghii Rroxscaffold_6G00406670
rosa_rugosa Rorug03G0145900
rosa_samantha Rh3AG195200 Rh3BG225300 Rh3CG220200 Rh3DG220700
rosa_wichuraiana Rw3G017780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 894
AciI CCGC 2 cut(s) 262, 894
AclWI GGATC 5 cut(s) 12, 1109, 1122, 1402, 1448
AcsI RAATTY 1 cut(s) 1255
AcuI CTGAAG 2 cut(s) 438, 710
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 3 cut(s) 118, 268, 1170
AgsI TTSAA 8 cut(s) 183, 208, 524, 532, 677, 854, 998, 1506
AjnI CCWGG 2 cut(s) 650, 1293
AleI CACNNNNGTG 1 cut(s) 1426
Alw21I GWGCWC 4 cut(s) 166, 330, 450, 778
Alw26I GTCTC 3 cut(s) 132, 554, 1320
AlwI GGATC 5 cut(s) 12, 1109, 1122, 1402, 1448
Ama87I CYCGRG 3 cut(s) 329, 542, 1110
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 4 cut(s) 300, 1442, 1478, 1537
ApeKI GCWGC 8 cut(s) 108, 428, 464, 760, 824, 926, 1106, 1435
ApoI RAATTY 1 cut(s) 1255
AseI ATTAAT 1 cut(s) 660
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 2 cut(s) 301, 1366
AsuHPI GGTGA 3 cut(s) 361, 977, 1544
AvaI CYCGRG 3 cut(s) 329, 542, 1110
AvaII GGWCC 1 cut(s) 1366
BamHI GGATCC 1 cut(s) 1114
BanII GRGCYC 6 cut(s) 142, 166, 330, 340, 778, 1129
BauI CACGAG 1 cut(s) 1449
Bbv12I GWGCWC 4 cut(s) 166, 330, 450, 778
BbvI GCAGC 8 cut(s) 120, 440, 451, 747, 811, 938, 1093, 1447
BccI CCATC 7 cut(s) 476, 966, 977, 995, 1116, 1211, 1492
BceAI ACGGC 2 cut(s) 1423, 1439
BcgI CGANNNNNNTGC 2 cut(s) 461, 495
BciT130I CCWGG 2 cut(s) 652, 1295
BcoDI GTCTC 3 cut(s) 132, 554, 1320
BfaI CTAG 7 cut(s) 153, 174, 294, 1386, 1401, 1413, 1517
BfmI CTRYAG 1 cut(s) 1101
BfoI RGCGCY 1 cut(s) 376
BglI GCCNNNNNGGC 1 cut(s) 1545
BisI GCNGC 8 cut(s) 109, 429, 465, 761, 825, 927, 1107, 1436
BlpI GCTNAGC 1 cut(s) 324
BlsI GCNGC 8 cut(s) 110, 430, 466, 762, 826, 928, 1108, 1437
Bme1390I CCNGG 2 cut(s) 652, 1295
Bme18I GGWCC 1 cut(s) 1366
BmeT110I CYCGRG 3 cut(s) 329, 542, 1110
BmgT120I GGNCC 2 cut(s) 301, 1366
BmiI GGNNCC 5 cut(s) 955, 1116, 1128, 1250, 1367
BmrFI CCNGG 2 cut(s) 652, 1295
BmsI GCATC 2 cut(s) 578, 732
BplI GAGNNNNNCTC 2 cut(s) 773, 805
BpmI CTGGAG 2 cut(s) 1108, 1316
Bpu10I CCTNAGC 1 cut(s) 59
Bpu1102I GCTNAGC 1 cut(s) 324
BpuEI CTTGAG 4 cut(s) 512, 617, 762, 830
Bsa29I ATCGAT 1 cut(s) 7
BsaJI CCNNGG 4 cut(s) 651, 949, 1439, 1492
BsaXI ACNNNNNCTCC 6 cut(s) 110, 140, 978, 1008, 1254, 1284
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 268, 1170
Bse1I ACTGG 2 cut(s) 558, 1091
BseBI CCWGG 2 cut(s) 652, 1295
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 4 cut(s) 651, 949, 1439, 1492
BseGI GGATG 3 cut(s) 38, 217, 988
BseLI CCNNNNNNNGG 3 cut(s) 118, 268, 1170
BseMII CTCAG 7 cut(s) 73, 179, 315, 324, 390, 500, 804
BseNI ACTGG 2 cut(s) 558, 1091
BseRI GAGGAG 3 cut(s) 827, 856, 1002
BseXI GCAGC 8 cut(s) 120, 440, 451, 747, 811, 938, 1093, 1447
BsgI GTGCAG 1 cut(s) 1485
Bsh1236I CGCG 1 cut(s) 894
BshFI GGCC 4 cut(s) 302, 1444, 1480, 1539
BshVI ATCGAT 1 cut(s) 7
BsiHKAI GWGCWC 4 cut(s) 166, 330, 450, 778
BsiHKCI CYCGRG 3 cut(s) 329, 542, 1110
BslFI GGGAC 1 cut(s) 1379
BslI CCNNNNNNNGG 3 cut(s) 118, 268, 1170
BsmAI GTCTC 3 cut(s) 132, 554, 1320
BsmBI CGTCTC 1 cut(s) 1320
BsmFI GGGAC 1 cut(s) 1379
BsmI GAATGC 2 cut(s) 826, 1042
BsnI GGCC 4 cut(s) 302, 1444, 1480, 1539
BsoBI CYCGRG 3 cut(s) 329, 542, 1110
Bsp1286I GDGCHC 7 cut(s) 142, 166, 330, 340, 450, 778, 1129
Bsp1720I GCTNAGC 1 cut(s) 324
Bsp19I CCATGG 1 cut(s) 1492
BspACI CCGC 2 cut(s) 262, 894
BspANI GGCC 4 cut(s) 302, 1444, 1480, 1539
BspCNI CTCAG 7 cut(s) 72, 178, 316, 323, 389, 501, 803
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 1 cut(s) 894
BspHI TCATGA 2 cut(s) 402, 712
BspLI GGNNCC 5 cut(s) 955, 1116, 1128, 1250, 1367
BspPI GGATC 5 cut(s) 12, 1109, 1122, 1402, 1448
BsrI ACTGG 2 cut(s) 558, 1091
BssECI CCNNGG 4 cut(s) 651, 949, 1439, 1492
BssSI CACGAG 1 cut(s) 1449
BssT1I CCWWGG 2 cut(s) 949, 1492
Bst2BI CACGAG 1 cut(s) 1449
Bst2UI CCWGG 2 cut(s) 652, 1295
Bst4CI ACNGT 2 cut(s) 187, 1563
Bst6I CTCTTC 3 cut(s) 602, 711, 1011
BstC8I GCNNGC 1 cut(s) 1235
BstDSI CCRYGG 2 cut(s) 1439, 1492
BstENI CCTNNNNNAGG 1 cut(s) 116
BstF5I GGATG 3 cut(s) 38, 217, 988
BstFNI CGCG 1 cut(s) 894
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstMAI GTCTC 3 cut(s) 132, 554, 1320
BstMWI GCNNNNNNNGC 6 cut(s) 320, 379, 1103, 1432, 1441, 1545
BstNI CCWGG 2 cut(s) 652, 1295
BstNSI RCATGY 2 cut(s) 323, 1237
BstSCI CCNGG 2 cut(s) 650, 1293
BstSFI CTRYAG 1 cut(s) 1101
BstUI CGCG 1 cut(s) 894
BstV1I GCAGC 8 cut(s) 120, 440, 451, 747, 811, 938, 1093, 1447
BstX2I RGATCY 3 cut(s) 1114, 1394, 1453
BstXI CCANNNNNNTGG 1 cut(s) 1499
BstYI RGATCY 3 cut(s) 1114, 1394, 1453
Bsu15I ATCGAT 1 cut(s) 7
BsuRI GGCC 4 cut(s) 302, 1444, 1480, 1539
BsuTUI ATCGAT 1 cut(s) 7
BtgI CCRYGG 2 cut(s) 1439, 1492
BtsCI GGATG 3 cut(s) 38, 217, 988
BtsI GCAGTG 2 cut(s) 1433, 1504
BtsIMutI CAGTG 2 cut(s) 1433, 1504
Cac8I GCNNGC 1 cut(s) 1235
CciI TCATGA 2 cut(s) 402, 712
CfoI GCGC 1 cut(s) 375
Cfr13I GGNCC 2 cut(s) 301, 1366
ClaI ATCGAT 1 cut(s) 7
CseI GACGC 1 cut(s) 900
Eam1104I CTCTTC 3 cut(s) 602, 711, 1011
EarI CTCTTC 3 cut(s) 602, 711, 1011
Ecl136II GAGCTC 3 cut(s) 164, 328, 776
Eco130I CCWWGG 2 cut(s) 949, 1492
Eco147I AGGCCT 1 cut(s) 1480
Eco24I GRGCYC 6 cut(s) 142, 166, 330, 340, 778, 1129
Eco47I GGWCC 1 cut(s) 1366
Eco47III AGCGCT 1 cut(s) 374
Eco53kI GAGCTC 3 cut(s) 164, 328, 776
Eco57I CTGAAG 2 cut(s) 438, 710
Eco88I CYCGRG 3 cut(s) 329, 542, 1110
EcoICRI GAGCTC 3 cut(s) 164, 328, 776
EcoNI CCTNNNNNAGG 1 cut(s) 116
EcoRII CCWGG 2 cut(s) 650, 1293
EcoT14I CCWWGG 2 cut(s) 949, 1492
EcoT38I GRGCYC 6 cut(s) 142, 166, 330, 340, 778, 1129
ErhI CCWWGG 2 cut(s) 949, 1492
Esp3I CGTCTC 1 cut(s) 1320
FalI AAGNNNNNCTT 6 cut(s) 657, 689, 928, 960, 1180, 1212
FaqI GGGAC 1 cut(s) 1379
Fnu4HI GCNGC 8 cut(s) 109, 429, 465, 761, 825, 927, 1107, 1436
FokI GGATG 3 cut(s) 25, 224, 995
FriOI GRGCYC 6 cut(s) 142, 166, 330, 340, 778, 1129
Fsp4HI GCNGC 8 cut(s) 109, 429, 465, 761, 825, 927, 1107, 1436
FspBI CTAG 7 cut(s) 153, 174, 294, 1386, 1401, 1413, 1517
GlaI GCGC 1 cut(s) 374
GluI GCNGC 8 cut(s) 109, 429, 465, 761, 825, 927, 1107, 1436
GsuI CTGGAG 2 cut(s) 1108, 1316
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 4 cut(s) 302, 1444, 1480, 1539
HgaI GACGC 1 cut(s) 900
HhaI GCGC 1 cut(s) 375
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 848
HindII GTYRAC 1 cut(s) 848
HindIII AAGCTT 1 cut(s) 526
HphI GGTGA 3 cut(s) 361, 977, 1544
Hpy166II GTNNAC 2 cut(s) 848, 1471
Hpy188I TCNGA 8 cut(s) 150, 168, 228, 379, 754, 793, 909, 1216
Hpy8I GTNNAC 2 cut(s) 848, 1471
HpyAV CCTTC 8 cut(s) 27, 202, 297, 507, 1348, 1455, 1529, 1545
HpyCH4III ACNGT 2 cut(s) 187, 1563
HpyCH4IV ACGT 2 cut(s) 202, 233
HpyF10VI GCNNNNNNNGC 6 cut(s) 320, 379, 1103, 1432, 1441, 1545
HpySE526I ACGT 2 cut(s) 202, 233
HspAI GCGC 1 cut(s) 373
LmnI GCTCC 3 cut(s) 445, 1132, 1254
Lsp1109I GCAGC 8 cut(s) 120, 440, 451, 747, 811, 938, 1093, 1447
LweI GCATC 2 cut(s) 578, 732
MaeI CTAG 7 cut(s) 153, 174, 294, 1386, 1401, 1413, 1517
MaeII ACGT 2 cut(s) 202, 233
MflI RGATCY 3 cut(s) 1114, 1394, 1453
MhlI GDGCHC 7 cut(s) 142, 166, 330, 340, 450, 778, 1129
MluCI AATT 4 cut(s) 252, 644, 1052, 1255
MlyI GAGTC 7 cut(s) 134, 139, 163, 482, 707, 853, 1099
MmeI TCCRAC 3 cut(s) 169, 173, 1442
MseI TTAA 3 cut(s) 579, 660, 1055
MslI CAYNNNNRTG 4 cut(s) 102, 963, 1426, 1497
MspR9I CCNGG 2 cut(s) 652, 1295
Mva1269I GAATGC 2 cut(s) 826, 1042
MvaI CCWGG 2 cut(s) 652, 1295
MvnI CGCG 1 cut(s) 894
MwoI GCNNNNNNNGC 6 cut(s) 320, 379, 1103, 1432, 1441, 1545
NcoI CCATGG 1 cut(s) 1492
NlaIV GGNNCC 5 cut(s) 955, 1116, 1128, 1250, 1367
NspI RCATGY 2 cut(s) 323, 1237
OliI CACNNNNGTG 1 cut(s) 1426
PaeI GCATGC 1 cut(s) 1237
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 2 cut(s) 402, 712
PceI AGGCCT 1 cut(s) 1480
PctI GAATGC 2 cut(s) 826, 1042
PfeI GAWTC 4 cut(s) 92, 192, 352, 1022
PflFI GACNNNGTC 1 cut(s) 232
PkrI GCNGC 8 cut(s) 110, 430, 466, 762, 826, 928, 1108, 1437
PleI GAGTC 7 cut(s) 133, 139, 163, 481, 706, 852, 1098
PpsI GAGTC 7 cut(s) 133, 139, 163, 481, 706, 852, 1098
PshBI ATTAAT 1 cut(s) 660
Psp124BI GAGCTC 3 cut(s) 166, 330, 778
Psp6I CCWGG 2 cut(s) 650, 1293
PspGI CCWGG 2 cut(s) 650, 1293
PspN4I GGNNCC 5 cut(s) 955, 1116, 1128, 1250, 1367
PspPI GGNCC 2 cut(s) 301, 1366
PsuI RGATCY 3 cut(s) 1114, 1394, 1453
PsyI GACNNNGTC 1 cut(s) 232
RseI CAYNNNNRTG 4 cut(s) 102, 963, 1426, 1497
SacI GAGCTC 3 cut(s) 166, 330, 778
SaqAI TTAA 3 cut(s) 579, 660, 1055
SatI GCNGC 8 cut(s) 109, 429, 465, 761, 825, 927, 1107, 1436
Sau96I GGNCC 2 cut(s) 301, 1366
SchI GAGTC 7 cut(s) 134, 139, 163, 482, 707, 853, 1099
ScrFI CCNGG 2 cut(s) 652, 1295
SduI GDGCHC 7 cut(s) 142, 166, 330, 340, 450, 778, 1129
SfaNI GCATC 2 cut(s) 578, 732
SfcI CTRYAG 1 cut(s) 1101
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 1366
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 4 cut(s) 102, 963, 1426, 1497
SmlI CTYRAG 5 cut(s) 329, 491, 596, 777, 809
SmoI CTYRAG 5 cut(s) 329, 491, 596, 777, 809
SphI GCATGC 1 cut(s) 1237
Sse9I AATT 4 cut(s) 252, 644, 1052, 1255
SseBI AGGCCT 1 cut(s) 1480
SsiI CCGC 2 cut(s) 262, 894
SspI AATATT 1 cut(s) 87
SspMI CTAG 7 cut(s) 153, 174, 294, 1386, 1401, 1413, 1517
SstI GAGCTC 3 cut(s) 166, 330, 778
StuI AGGCCT 1 cut(s) 1480
StyD4I CCNGG 2 cut(s) 650, 1293
StyI CCWWGG 2 cut(s) 949, 1492
TaaI ACNGT 2 cut(s) 187, 1563
TaiI ACGT 2 cut(s) 205, 236
TaqI TCGA 7 cut(s) 7, 130, 158, 330, 471, 723, 1352
TasI AATT 4 cut(s) 252, 644, 1052, 1255
TfiI GAWTC 4 cut(s) 92, 192, 352, 1022
Tru1I TTAA 3 cut(s) 579, 660, 1055
Tru9I TTAA 3 cut(s) 579, 660, 1055
TscAI CASTG 2 cut(s) 1433, 1504
TseI GCWGC 8 cut(s) 108, 428, 464, 760, 824, 926, 1106, 1435
TspDTI ATGAA 9 cut(s) 279, 291, 419, 657, 678, 701, 729, 930, 1397
TspRI CASTG 2 cut(s) 1433, 1504
Tth111I GACNNNGTC 1 cut(s) 232
VpaK11BI GGWCC 1 cut(s) 1366
VspI ATTAAT 1 cut(s) 660
XagI CCTNNNNNAGG 1 cut(s) 116
XapI RAATTY 1 cut(s) 1255
XceI RCATGY 2 cut(s) 323, 1237
XcmI CCANNNNNNNNNTGG 2 cut(s) 1376, 1425
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 7 cut(s) 153, 174, 294, 1386, 1401, 1413, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.