MD12G1034000.v1.1

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013917
Physical Location & Seq
Forward (+)
3730706 .. 3734423
3718 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1034000.v1.1.491

Sequence Viewer

Length: 441 bp
ATGACGCTGGGCTCAGGAGGATCGAGTGTCGTGGTCCCACGAAACTTCAGATTGCTGGAGGAACTTGAACGTGGAGAAAAAGGTATTGGGGATGGCACTGTCAGCTATGGGATGGATGACGGAGATGACATCTACATGCGTTCTTGGACTGGCACCATCATTGGTCCCCACAATACTGTACATGAAGGTCGGATCTATCAGTTGAAATTGTTCTGTGATAAAGATTACCCTGAGAAGCCACCAAGTGTTCGTTTTCATTCTCGGGTTAACATGACTTGTGTTAACCATGAAACTGGAGTGGTGGAACCAAAGAAGTTTGGACTTCTTGCAAACTGGCAGCGAGAGTACACCATGGAAGATATTCTGACACAGCTGAAAAAGGAGATGGGAGCCCCACATAACCGGAAGTTGGTCCAGCCTCCAGAAGGTACCTACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

147

Amino Acids

16.57

Weight (kDa)

6.2

Isoelectric Point (pI)

26.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 44 - 136 4.9e-14 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52560 AT3G52560 AT3G52560 AT3G52560
fragaria_vesca FvH4_6g19850
malus_domestica MD12G1034000.v1.1 MD14G1035400.v1.1
prunus_persica Prupe.7G098800_v2.0.a1 Prupe.7G098800_v2.0.a1
pyrus_communis pycom14g03110
rosa_chinensis RchiOBHm_Chr3g0475251
rosa_laevigata RLG00000023856
rosa_multiflora Rmu_co8404831.1_g000001 Rmu_sc0003093.1_g000044
rosa_roxburghii Rroxscaffold_6G00406430
rosa_rugosa Rorug03G0147500
rosa_wichuraiana Rw3G017950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 428
AccB1I GGYRCC 2 cut(s) 152, 428
AclWI GGATC 2 cut(s) 28, 200
AcuI CTGAAG 1 cut(s) 31
AdeI CACNNNGTG 1 cut(s) 245
AfaI GTAC 3 cut(s) 180, 347, 430
AfiI CCNNNNNNNGG 2 cut(s) 409, 425
AgsI TTSAA 2 cut(s) 68, 205
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 2 cut(s) 105, 373
AluI AGCT 2 cut(s) 105, 373
AlwI GGATC 2 cut(s) 28, 200
Ama87I CYCGRG 1 cut(s) 261
ApeKI GCWGC 1 cut(s) 337
Asp700I GAANNNNTTC 2 cut(s) 209, 360
Asp718I GGTACC 1 cut(s) 428
AspS9I GGNCC 3 cut(s) 34, 164, 412
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 3 cut(s) 34, 164, 412
BanI GGYRCC 2 cut(s) 152, 428
BanII GRGCYC 2 cut(s) 14, 394
BbvI GCAGC 1 cut(s) 349
BccI CCATC 4 cut(s) 86, 106, 164, 379
BfaI CTAG 1 cut(s) 439
BisI GCNGC 1 cut(s) 338
BlsI GCNGC 1 cut(s) 339
Bme18I GGWCC 3 cut(s) 34, 164, 412
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 3 cut(s) 34, 164, 412
BmiI GGNNCC 6 cut(s) 36, 154, 166, 306, 391, 430
BpmI CTGGAG 3 cut(s) 77, 315, 405
Bpu10I CCTNAGC 1 cut(s) 13
BsaJI CCNNGG 1 cut(s) 351
BsaWI WCCGGW 1 cut(s) 402
Bsc4I CCNNNNNNNGG 2 cut(s) 409, 425
Bse1I ACTGG 3 cut(s) 154, 298, 338
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 3 cut(s) 97, 117, 121
BseLI CCNNNNNNNGG 2 cut(s) 409, 425
BseMII CTCAG 2 cut(s) 27, 222
BseNI ACTGG 3 cut(s) 154, 298, 338
BseXI GCAGC 1 cut(s) 349
BseYI CCCAGC 1 cut(s) 7
BshNI GGYRCC 2 cut(s) 152, 428
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 1 cut(s) 403
BslFI GGGAC 2 cut(s) 20, 150
BslI CCNNNNNNNGG 2 cut(s) 409, 425
BsmFI GGGAC 2 cut(s) 20, 150
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 2 cut(s) 14, 394
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 2 cut(s) 20, 192
Bsp19I CCATGG 1 cut(s) 351
BspCNI CTCAG 2 cut(s) 26, 223
BspLI GGNNCC 6 cut(s) 36, 154, 166, 306, 391, 430
BspPI GGATC 2 cut(s) 28, 200
BspT107I GGYRCC 2 cut(s) 152, 428
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 3 cut(s) 154, 298, 338
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 2 cut(s) 20, 192
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 2 cut(s) 100, 178
BstAUI TGTACA 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 13, 231
BstDSI CCRYGG 1 cut(s) 351
BstENI CCTNNNNNAGG 1 cut(s) 423
BstF5I GGATG 3 cut(s) 97, 117, 121
BstKTI GATC 2 cut(s) 23, 195
BstMBI GATC 2 cut(s) 20, 192
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 139
BstV1I GCAGC 1 cut(s) 349
BstX2I RGATCY 1 cut(s) 192
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 97, 117, 121
BtsIMutI CAGTG 1 cut(s) 96
Cfr13I GGNCC 3 cut(s) 34, 164, 412
CseI GACGC 1 cut(s) 13
Csp6I GTAC 3 cut(s) 179, 346, 429
CviAII CATG 5 cut(s) 136, 182, 271, 287, 352
CviJI RGCY 6 cut(s) 12, 105, 238, 373, 392, 418
CviKI_1 RGCY 6 cut(s) 12, 105, 238, 373, 392, 418
CviQI GTAC 3 cut(s) 179, 346, 429
DdeI CTNAG 2 cut(s) 13, 231
DpnI GATC 2 cut(s) 22, 194
DpnII GATC 2 cut(s) 20, 192
DraIII CACNNNGTG 1 cut(s) 245
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 2 cut(s) 14, 394
Eco47I GGWCC 3 cut(s) 34, 164, 412
Eco57I CTGAAG 1 cut(s) 31
Eco88I CYCGRG 1 cut(s) 261
EcoNI CCTNNNNNAGG 1 cut(s) 423
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 2 cut(s) 14, 394
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 5 cut(s) 139, 185, 274, 290, 355
FaiI YATR 7 cut(s) 108, 137, 183, 272, 288, 353, 399
FaqI GGGAC 2 cut(s) 20, 150
FatI CATG 5 cut(s) 135, 181, 270, 286, 351
Fnu4HI GCNGC 1 cut(s) 338
FokI GGATG 3 cut(s) 104, 124, 128
FriOI GRGCYC 2 cut(s) 14, 394
Fsp4HI GCNGC 1 cut(s) 338
FspBI CTAG 1 cut(s) 439
GluI GCNGC 1 cut(s) 338
GsaI CCCAGC 1 cut(s) 11
GsuI CTGGAG 3 cut(s) 77, 315, 405
HapII CCGG 1 cut(s) 403
HgaI GACGC 1 cut(s) 13
Hin1II CATG 5 cut(s) 139, 185, 274, 290, 355
HincII GTYRAC 2 cut(s) 268, 283
HindII GTYRAC 2 cut(s) 268, 283
HpaI GTTAAC 2 cut(s) 268, 283
HpaII CCGG 1 cut(s) 403
Hpy166II GTNNAC 3 cut(s) 268, 283, 348
Hpy188I TCNGA 3 cut(s) 50, 192, 366
Hpy188III TCNNGA 2 cut(s) 15, 422
Hpy8I GTNNAC 3 cut(s) 268, 283, 348
HpyAV CCTTC 2 cut(s) 179, 419
HpyCH4III ACNGT 2 cut(s) 100, 178
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 1 cut(s) 329
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 2 cut(s) 13, 231
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 139, 185, 274, 290, 355
KpnI GGTACC 1 cut(s) 432
KspAI GTTAAC 2 cut(s) 268, 283
Kzo9I GATC 2 cut(s) 20, 192
LmnI GCTCC 1 cut(s) 389
LpnPI CCDG 8 cut(s) 41, 135, 243, 279, 319, 416, 428, 435
Lsp1109I GCAGC 1 cut(s) 349
MaeI CTAG 1 cut(s) 439
MaeII ACGT 1 cut(s) 70
MalI GATC 2 cut(s) 22, 194
MboI GATC 2 cut(s) 20, 192
MboII GAAGA 1 cut(s) 368
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 14, 394
MluCI AATT 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 3 cut(s) 11, 52, 429
MroXI GAANNNNTTC 2 cut(s) 209, 360
MseI TTAA 2 cut(s) 267, 282
MslI CAYNNNNRTG 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 1 cut(s) 403
MwoI GCNNNNNNNGC 1 cut(s) 102
NcoI CCATGG 1 cut(s) 351
NdeII GATC 2 cut(s) 20, 192
NlaIII CATG 5 cut(s) 139, 185, 274, 290, 355
NlaIV GGNNCC 6 cut(s) 36, 154, 166, 306, 391, 430
NspI RCATGY 1 cut(s) 139
PdmI GAANNNNTTC 2 cut(s) 209, 360
PkrI GCNGC 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 7
PspN4I GGNNCC 6 cut(s) 36, 154, 166, 306, 391, 430
PspPI GGNCC 3 cut(s) 34, 164, 412
PsuI RGATCY 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 3 cut(s) 180, 347, 430
RsaNI GTAC 3 cut(s) 179, 346, 429
RseI CAYNNNNRTG 1 cut(s) 134
SaqAI TTAA 2 cut(s) 267, 282
SatI GCNGC 1 cut(s) 338
Sau3AI GATC 2 cut(s) 20, 192
Sau96I GGNCC 3 cut(s) 34, 164, 412
SduI GDGCHC 2 cut(s) 14, 394
SetI ASST 7 cut(s) 73, 85, 107, 190, 375, 430, 434
SinI GGWCC 3 cut(s) 34, 164, 412
SmiMI CAYNNNNRTG 1 cut(s) 134
Sse9I AATT 1 cut(s) 206
SspMI CTAG 1 cut(s) 439
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 2 cut(s) 100, 178
TaiI ACGT 1 cut(s) 73
TaqI TCGA 1 cut(s) 23
TasI AATT 1 cut(s) 206
TatI WGTACW 2 cut(s) 178, 345
Tru1I TTAA 2 cut(s) 267, 282
Tru9I TTAA 2 cut(s) 267, 282
TscAI CASTG 1 cut(s) 103
TseI GCWGC 1 cut(s) 337
TspDTI ATGAA 3 cut(s) 198, 245, 303
TspGWI ACGGA 1 cut(s) 135
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 3 cut(s) 34, 164, 412
XagI CCTNNNNNAGG 1 cut(s) 423
XceI RCATGY 1 cut(s) 139
XmnI GAANNNNTTC 2 cut(s) 209, 360
XspI CTAG 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.