RLG00000023856

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013917
Physical Location & Seq
Reverse (-)
29207937 .. 29211823
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023856

Sequence Viewer

Length: 435 bp
ATGACTCTAGGCTCAGGAGGATCCGGTGTTGTGGTCCCAAGAAATTTCAGATTGCTGGAGGAACTTGAACGTGGGGAGAAAGGTATTGGGGATGGCACTGTGAGCTATGGGATGGATGACGGAGATGATATCTACATGCGTTCTTGGACTGGCACCATCATTGGTCCCCATAATACTGTACATGAAGGTCGGATCTATCAGTTGAAGCTGTTTTGTGATAAAGATTACCCAGAGAAGCCACCAACTGTTCGATTTCATTCACGGATTAACATGACTTGTGTCAACCATGAAACTGGAGTGGTGGATTCGAAGAAGTTTGGGCTTCTTGCAAACTGGCAGAGAGAGTACACCATGGAGGATATTCTTACACAGCTGAAAAAGGAGATGGCAGCTGCACATAACCGGAAGTTGGTCCAGCCCCCGGAAGGATCCTAG

Protein Analysis

145

Amino Acids

16.21

Weight (kDa)

6.2

Isoelectric Point (pI)

22.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 44 - 131 1.6e-14 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013917)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52560 AT3G52560 AT3G52560 AT3G52560
fragaria_vesca FvH4_6g19850
malus_domestica MD12G1034000.v1.1 MD14G1035400.v1.1
prunus_persica Prupe.7G098800_v2.0.a1 Prupe.7G098800_v2.0.a1
pyrus_communis pycom14g03110
rosa_chinensis RchiOBHm_Chr3g0475251
rosa_laevigata RLG00000023856
rosa_multiflora Rmu_co8404831.1_g000001 Rmu_sc0003093.1_g000044
rosa_roxburghii Rroxscaffold_6G00406430
rosa_rugosa Rorug03G0147500
rosa_wichuraiana Rw3G017950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 152
AclWI GGATC 4 cut(s) 15, 28, 200, 423
AcsI RAATTY 1 cut(s) 43
AfaI GTAC 2 cut(s) 180, 347
AfiI CCNNNNNNNGG 3 cut(s) 409, 421, 425
AgsI TTSAA 2 cut(s) 68, 205
AluBI AGCT 4 cut(s) 105, 208, 373, 392
AluI AGCT 4 cut(s) 105, 208, 373, 392
AlwI GGATC 4 cut(s) 15, 28, 200, 423
ApeKI GCWGC 2 cut(s) 389, 392
ApoI RAATTY 1 cut(s) 43
AspS9I GGNCC 3 cut(s) 34, 164, 412
AsuC2I CCSGG 1 cut(s) 422
AsuII TTCGAA 1 cut(s) 308
AvaII GGWCC 3 cut(s) 34, 164, 412
BamHI GGATCC 2 cut(s) 20, 428
BanI GGYRCC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 379, 401
BccI CCATC 4 cut(s) 86, 106, 164, 379
BcnI CCSGG 1 cut(s) 422
BfaI CTAG 2 cut(s) 8, 433
BisI GCNGC 2 cut(s) 390, 393
BlsI GCNGC 2 cut(s) 391, 394
Bme1390I CCNGG 1 cut(s) 422
Bme18I GGWCC 3 cut(s) 34, 164, 412
BmgT120I GGNCC 3 cut(s) 34, 164, 412
BmiI GGNNCC 5 cut(s) 22, 36, 154, 166, 430
BmrFI CCNGG 1 cut(s) 422
BoxI GACNNNNGTC 1 cut(s) 278
BpmI CTGGAG 2 cut(s) 77, 315
Bpu10I CCTNAGC 1 cut(s) 13
Bpu14I TTCGAA 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 422
BsaJI CCNNGG 2 cut(s) 351, 420
BsaWI WCCGGW 2 cut(s) 23, 402
Bsc4I CCNNNNNNNGG 3 cut(s) 409, 421, 425
Bse1I ACTGG 3 cut(s) 154, 298, 338
BseDI CCNNGG 2 cut(s) 351, 420
BseGI GGATG 3 cut(s) 97, 117, 121
BseLI CCNNNNNNNGG 3 cut(s) 409, 421, 425
BseMII CTCAG 1 cut(s) 27
BseNI ACTGG 3 cut(s) 154, 298, 338
BseXI GCAGC 2 cut(s) 379, 401
BsgI GTGCAG 1 cut(s) 378
BshNI GGYRCC 1 cut(s) 152
BsiSI CCGG 3 cut(s) 24, 403, 422
BslFI GGGAC 2 cut(s) 20, 150
BslI CCNNNNNNNGG 3 cut(s) 409, 421, 425
BsmFI GGGAC 2 cut(s) 20, 150
Bsp119I TTCGAA 1 cut(s) 308
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 3 cut(s) 20, 192, 428
Bsp19I CCATGG 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 26
BspLI GGNNCC 5 cut(s) 22, 36, 154, 166, 430
BspPI GGATC 4 cut(s) 15, 28, 200, 423
BspT104I TTCGAA 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 3 cut(s) 154, 298, 338
BssECI CCNNGG 2 cut(s) 351, 420
BssMI GATC 3 cut(s) 20, 192, 428
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 3 cut(s) 100, 178, 247
BstAUI TGTACA 1 cut(s) 178
BstBI TTCGAA 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 13
BstDSI CCRYGG 1 cut(s) 351
BstF5I GGATG 3 cut(s) 97, 117, 121
BstKTI GATC 3 cut(s) 23, 195, 431
BstMBI GATC 3 cut(s) 20, 192, 428
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 139
BstPAI GACNNNNGTC 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 420
BstV1I GCAGC 2 cut(s) 379, 401
BstX2I RGATCY 3 cut(s) 20, 192, 428
BstXI CCANNNNNNTGG 1 cut(s) 293
BstYI RGATCY 3 cut(s) 20, 192, 428
BtgI CCRYGG 1 cut(s) 351
BtsCI GGATG 3 cut(s) 97, 117, 121
BtsIMutI CAGTG 1 cut(s) 96
Cfr13I GGNCC 3 cut(s) 34, 164, 412
Csp6I GTAC 2 cut(s) 179, 346
CviAII CATG 5 cut(s) 136, 182, 271, 287, 352
CviJI RGCY 8 cut(s) 12, 105, 208, 238, 322, 373, 392, 418
CviKI_1 RGCY 8 cut(s) 12, 105, 208, 238, 322, 373, 392, 418
CviQI GTAC 2 cut(s) 179, 346
DdeI CTNAG 1 cut(s) 13
DpnI GATC 3 cut(s) 22, 194, 430
DpnII GATC 3 cut(s) 20, 192, 428
Eco130I CCWWGG 1 cut(s) 351
Eco32I GATATC 1 cut(s) 130
Eco47I GGWCC 3 cut(s) 34, 164, 412
EcoRV GATATC 1 cut(s) 130
EcoT14I CCWWGG 1 cut(s) 351
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 5 cut(s) 139, 185, 274, 290, 355
FaiI YATR 8 cut(s) 108, 137, 171, 183, 272, 288, 353, 399
FaqI GGGAC 2 cut(s) 20, 150
FatI CATG 5 cut(s) 135, 181, 270, 286, 351
Fnu4HI GCNGC 2 cut(s) 390, 393
FokI GGATG 3 cut(s) 104, 124, 128
Fsp4HI GCNGC 2 cut(s) 390, 393
FspBI CTAG 2 cut(s) 8, 433
GluI GCNGC 2 cut(s) 390, 393
GsuI CTGGAG 2 cut(s) 77, 315
HapII CCGG 3 cut(s) 24, 403, 422
Hin1II CATG 5 cut(s) 139, 185, 274, 290, 355
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HinfI GANTC 2 cut(s) 4, 305
HpaII CCGG 3 cut(s) 24, 403, 422
Hpy166II GTNNAC 2 cut(s) 283, 348
Hpy188I TCNGA 2 cut(s) 50, 192
Hpy188III TCNNGA 1 cut(s) 15
Hpy8I GTNNAC 2 cut(s) 283, 348
HpyAV CCTTC 2 cut(s) 179, 419
HpyCH4III ACNGT 3 cut(s) 100, 178, 247
HpyCH4IV ACGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 329, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 1 cut(s) 13
HpySE526I ACGT 1 cut(s) 70
Hsp92II CATG 5 cut(s) 139, 185, 274, 290, 355
Kzo9I GATC 3 cut(s) 20, 192, 428
LpnPI CCDG 8 cut(s) 37, 41, 135, 243, 279, 319, 416, 428
Lsp1109I GCAGC 2 cut(s) 379, 401
MaeI CTAG 2 cut(s) 8, 433
MaeII ACGT 1 cut(s) 70
MalI GATC 3 cut(s) 22, 194, 430
MboI GATC 3 cut(s) 20, 192, 428
MboII GAAGA 1 cut(s) 322
MflI RGATCY 3 cut(s) 20, 192, 428
MluCI AATT 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 170
MnlI CCTC 3 cut(s) 11, 52, 349
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 2 cut(s) 373, 392
MspI CCGG 3 cut(s) 24, 403, 422
MspR9I CCNGG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 102
NciI CCSGG 1 cut(s) 422
NcoI CCATGG 1 cut(s) 351
NdeII GATC 3 cut(s) 20, 192, 428
NlaIII CATG 5 cut(s) 139, 185, 274, 290, 355
NlaIV GGNNCC 5 cut(s) 22, 36, 154, 166, 430
NspI RCATGY 1 cut(s) 139
NspV TTCGAA 1 cut(s) 308
PfeI GAWTC 1 cut(s) 305
PkrI GCNGC 2 cut(s) 391, 394
PshAI GACNNNNGTC 1 cut(s) 278
PspN4I GGNNCC 5 cut(s) 22, 36, 154, 166, 430
PspPI GGNCC 3 cut(s) 34, 164, 412
PsuI RGATCY 3 cut(s) 20, 192, 428
PvuII CAGCTG 2 cut(s) 373, 392
RsaI GTAC 2 cut(s) 180, 347
RsaNI GTAC 2 cut(s) 179, 346
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 390, 393
Sau3AI GATC 3 cut(s) 20, 192, 428
Sau96I GGNCC 3 cut(s) 34, 164, 412
ScrFI CCNGG 1 cut(s) 422
SetI ASST 7 cut(s) 73, 85, 107, 190, 210, 375, 394
SfuI TTCGAA 1 cut(s) 308
SinI GGWCC 3 cut(s) 34, 164, 412
Sse9I AATT 1 cut(s) 43
SspMI CTAG 2 cut(s) 8, 433
StyD4I CCNGG 1 cut(s) 420
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 100, 178, 247
TaiI ACGT 1 cut(s) 73
TaqI TCGA 2 cut(s) 250, 308
TasI AATT 1 cut(s) 43
TatI WGTACW 2 cut(s) 178, 345
TfiI GAWTC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 103
TseI GCWGC 2 cut(s) 389, 392
TspDTI ATGAA 3 cut(s) 198, 245, 303
TspGWI ACGGA 2 cut(s) 135, 277
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 3 cut(s) 34, 164, 412
XapI RAATTY 1 cut(s) 43
XceI RCATGY 1 cut(s) 139
XspI CTAG 2 cut(s) 8, 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.