MD12G1041000.v1.1

Zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016059
Physical Location & Seq
Reverse (-)
4555425 .. 4556378
954 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1041000.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGAGAGAGCAGGATGGAAACCAAACGATGATCATCATCACATATCTCCCAATTGTCCCCGGTGTGGTTGTTCCAACACCAAATTTTGTTACTACAACAACTATAGTTTAACTCAGCCAAGGTACTTTTGCAAGGGGTGTAGGAGGTACTGGACCAAAGGAGGGTCCCTCAGGAACGTCCCCGTCGGAGGTGGCTGCCGCAGGAATAGAAGAGGGTCCAAGTCTTTAAGGCTATCCGCCACCTACACCACCAGCACTACAGAAGGTCACGCGCATCACAACACAAATAAGAGTAGGGCCAGTACTTATGAGGGTGCTCATAATTCCTATGGCACCAACGTGTTGGGTGATTCCACAAGTCCCTCAGTGACTCAAGATGGGTCACATATTGATCTTGCTCTTGTTTATGCAAATTTCTTGAAGCAGAAGCCCGATAATTCAGATAGTACTACCGGATCTGATCATCAACTACCAGAATTGCCAAGCGAATTCGACCCGGCAGCTTTAGATTTTTCAAGCATGGCATTAAACATGAACAATGCAAGCTCAGACAATACTAGTACTCAGGTAATGGATGATCATTATCATCAAAATGGTCTTGTTGGGTCGCTTGGATGTCACACTCTATCTCATGATCACTTGTCCACTGAAAATTGTAGCACCAATGACCGTATGTACTTTGGTGAATTAGACCCATTAATTCATCATCCAGAGAAGCATCATCATCAACACACAAGTGAAGTAGTACAATATGCAAGCGATTTTGGGTTGCCCCCATTGCCAGGCCAAGAGGCATTGTGGTCTACTACTTCTCAAATGATGGATTGTGGTAGTCATACAATGCAAGCTGGTACATCGCTACTAGGACATGAACTTCATAATCCAAACTCGTTAATGGGTAGTTGGAGCCCGTTTGATTTGCCATGTAACGAAACTTTCTCGAGGAGTACATGA

Protein Analysis

318

Amino Acids

35.09

Weight (kDa)

6.26

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 18 - 71 1.8e-29 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52440 AT3G52440
fragaria_vesca FvH4_6g20401
malus_domestica MD12G1041000.v1.1 MD14G1040400.v1.1
prunus_persica Prupe.7G093900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0476121
rosa_laevigata RLG00000023789
rosa_multiflora Rmu_sc0000114.1_g000027
rosa_roxburghii Rroxscaffold_6G00405460
rosa_rugosa Rorug03G0152900
rosa_samantha Rh3BG234200 Rh3CG229600 Rh3DG229600
rosa_wichuraiana Rw3G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 803
AccII CGCG 1 cut(s) 272
AciI CCGC 2 cut(s) 199, 237
AclWI GGATC 1 cut(s) 463
AcsI RAATTY 3 cut(s) 83, 412, 488
AfaI GTAC 9 cut(s) 125, 149, 304, 448, 562, 677, 747, 853, 949
AfiI CCNNNNNNNGG 4 cut(s) 65, 162, 188, 782
AflIII ACRYGT 1 cut(s) 339
AgsI TTSAA 2 cut(s) 421, 516
AhlI ACTAGT 1 cut(s) 557
AjnI CCWGG 1 cut(s) 781
AleI CACNNNNGTG 2 cut(s) 338, 735
AluBI AGCT 3 cut(s) 503, 546, 848
AluI AGCT 3 cut(s) 503, 546, 848
Alw21I GWGCWC 1 cut(s) 319
AlwI GGATC 1 cut(s) 463
Ama87I CYCGRG 1 cut(s) 940
AoxI GGCC 2 cut(s) 297, 784
ApeKI GCWGC 2 cut(s) 195, 500
ApoI RAATTY 3 cut(s) 83, 412, 488
AseI ATTAAT 1 cut(s) 698
AspLEI GCGC 1 cut(s) 274
AspS9I GGNCC 4 cut(s) 153, 165, 216, 297
AsuC2I CCSGG 2 cut(s) 61, 497
AsuHPI GGTGA 2 cut(s) 359, 695
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 3 cut(s) 153, 165, 216
AxyI CCTNAGG 1 cut(s) 170
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 911
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 182, 512
BccI CCATC 3 cut(s) 9, 371, 814
BciT130I CCWGG 1 cut(s) 783
BclI TGATCA 4 cut(s) 31, 460, 577, 634
BcnI CCSGG 2 cut(s) 61, 497
BcuI ACTAGT 1 cut(s) 557
BfaI CTAG 2 cut(s) 558, 863
BfmI CTRYAG 2 cut(s) 103, 258
BisI GCNGC 3 cut(s) 196, 199, 501
BlsI GCNGC 3 cut(s) 197, 200, 502
BmcAI AGTACT 3 cut(s) 304, 448, 562
Bme1390I CCNGG 3 cut(s) 61, 497, 783
Bme18I GGWCC 3 cut(s) 153, 165, 216
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 153, 165, 216, 297
BmiI GGNNCC 5 cut(s) 166, 167, 217, 334, 908
BmrFI CCNGG 3 cut(s) 61, 497, 783
BmsI GCATC 2 cut(s) 283, 727
BplI GAGNNNNNCTC 2 cut(s) 153, 185
BpuEI CTTGAG 1 cut(s) 357
BpuMI CCSGG 2 cut(s) 61, 497
BsaBI GATNNNNATC 3 cut(s) 33, 36, 582
BsaJI CCNNGG 2 cut(s) 59, 119
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 162, 188, 782
Bse1I ACTGG 2 cut(s) 155, 300
Bse21I CCTNAGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 776
Bse8I GATNNNNATC 3 cut(s) 33, 36, 582
BseBI CCWGG 1 cut(s) 783
BseDI CCNNGG 2 cut(s) 59, 119
BseGI GGATG 4 cut(s) 20, 580, 620, 706
BseJI GATNNNNATC 3 cut(s) 33, 36, 582
BseLI CCNNNNNNNGG 4 cut(s) 65, 162, 188, 782
BseMI GCAATG 1 cut(s) 776
BseMII CTCAG 5 cut(s) 128, 184, 378, 561, 578
BseNI ACTGG 2 cut(s) 155, 300
BseXI GCAGC 2 cut(s) 182, 512
Bsh1236I CGCG 1 cut(s) 272
BshFI GGCC 2 cut(s) 299, 786
BshNI GGYRCC 1 cut(s) 332
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 3 cut(s) 61, 453, 497
BslFI GGGAC 4 cut(s) 42, 151, 164, 345
BslI CCNNNNNNNGG 4 cut(s) 65, 162, 188, 782
BsmFI GGGAC 4 cut(s) 42, 151, 164, 345
BsnI GGCC 2 cut(s) 299, 786
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 2 cut(s) 319, 911
Bsp143I GATC 6 cut(s) 31, 391, 455, 460, 577, 634
BspACI CCGC 2 cut(s) 199, 237
BspANI GGCC 2 cut(s) 299, 786
BspCNI CTCAG 5 cut(s) 127, 183, 377, 560, 577
BspFNI CGCG 1 cut(s) 272
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 5 cut(s) 166, 167, 217, 334, 908
BspPI GGATC 1 cut(s) 463
BspT107I GGYRCC 1 cut(s) 332
BsrDI GCAATG 1 cut(s) 776
BsrI ACTGG 2 cut(s) 155, 300
BssECI CCNNGG 2 cut(s) 59, 119
BssMI GATC 6 cut(s) 31, 391, 455, 460, 577, 634
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 1 cut(s) 783
Bst4CI ACNGT 1 cut(s) 671
Bst6I CTCTTC 1 cut(s) 205
BstC8I GCNNGC 3 cut(s) 544, 757, 846
BstDEI CTNAG 5 cut(s) 114, 170, 364, 547, 564
BstF5I GGATG 4 cut(s) 20, 580, 620, 706
BstFNI CGCG 1 cut(s) 272
BstHHI GCGC 1 cut(s) 274
BstKTI GATC 6 cut(s) 34, 394, 458, 463, 580, 637
BstMBI GATC 6 cut(s) 31, 391, 455, 460, 577, 634
BstMWI GCNNNNNNNGC 1 cut(s) 778
BstNI CCWGG 1 cut(s) 783
BstSCI CCNGG 3 cut(s) 59, 495, 781
BstSFI CTRYAG 2 cut(s) 103, 258
BstUI CGCG 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 182, 512
BstX2I RGATCY 1 cut(s) 455
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 1 cut(s) 455
Bsu36I CCTNAGG 1 cut(s) 170
BsuRI GGCC 2 cut(s) 299, 786
BtgZI GCGATG 1 cut(s) 840
BtsCI GGATG 4 cut(s) 20, 580, 620, 706
BtsIMutI CAGTG 2 cut(s) 372, 645
Cac8I GCNNGC 3 cut(s) 544, 757, 846
CciI TCATGA 1 cut(s) 631
CfoI GCGC 1 cut(s) 274
Cfr13I GGNCC 4 cut(s) 153, 165, 216, 297
Csp6I GTAC 9 cut(s) 124, 148, 303, 447, 561, 676, 746, 852, 948
CviAII CATG 6 cut(s) 520, 532, 632, 869, 924, 951
CviQI GTAC 9 cut(s) 124, 148, 303, 447, 561, 676, 746, 852, 948
DdeI CTNAG 5 cut(s) 114, 170, 364, 547, 564
DpnI GATC 6 cut(s) 33, 393, 457, 462, 579, 636
DpnII GATC 6 cut(s) 31, 391, 455, 460, 577, 634
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 911
Eco47I GGWCC 3 cut(s) 153, 165, 216
Eco81I CCTNAGG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 940
EcoO109I RGGNCCY 1 cut(s) 165
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 1 cut(s) 781
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 6 cut(s) 523, 535, 635, 872, 927, 954
FaqI GGGAC 4 cut(s) 42, 151, 164, 345
FatI CATG 6 cut(s) 519, 531, 631, 868, 923, 950
FbaI TGATCA 4 cut(s) 31, 460, 577, 634
FblI GTMKAC 1 cut(s) 803
Fnu4HI GCNGC 3 cut(s) 196, 199, 501
FokI GGATG 4 cut(s) 27, 587, 627, 693
FriOI GRGCYC 1 cut(s) 911
Fsp4HI GCNGC 3 cut(s) 196, 199, 501
FspBI CTAG 2 cut(s) 558, 863
GlaI GCGC 1 cut(s) 273
GluI GCNGC 3 cut(s) 196, 199, 501
HaeIII GGCC 2 cut(s) 299, 786
HapII CCGG 3 cut(s) 61, 453, 497
HhaI GCGC 1 cut(s) 274
Hin1II CATG 6 cut(s) 523, 535, 635, 872, 927, 954
Hin6I GCGC 1 cut(s) 272
HinP1I GCGC 1 cut(s) 272
HinfI GANTC 2 cut(s) 350, 370
HpaII CCGG 3 cut(s) 61, 453, 497
HphI GGTGA 2 cut(s) 359, 695
Hpy166II GTNNAC 2 cut(s) 645, 804
Hpy188I TCNGA 4 cut(s) 188, 442, 460, 550
Hpy188III TCNNGA 6 cut(s) 172, 374, 418, 632, 710, 940
Hpy8I GTNNAC 2 cut(s) 645, 804
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 1 cut(s) 671
HpyCH4IV ACGT 2 cut(s) 177, 339
HpyCH4V TGCA 5 cut(s) 132, 410, 542, 755, 844
HpyF10VI GCNNNNNNNGC 1 cut(s) 778
HpyF3I CTNAG 5 cut(s) 114, 170, 364, 547, 564
HpySE526I ACGT 2 cut(s) 177, 339
Hsp92II CATG 6 cut(s) 523, 535, 635, 872, 927, 954
HspAI GCGC 1 cut(s) 272
KflI GGGWCCC 1 cut(s) 165
Ksp22I TGATCA 4 cut(s) 31, 460, 577, 634
Kzo9I GATC 6 cut(s) 31, 391, 455, 460, 577, 634
LmnI GCTCC 1 cut(s) 906
Lsp1109I GCAGC 2 cut(s) 182, 512
LweI GCATC 2 cut(s) 283, 727
MaeI CTAG 2 cut(s) 558, 863
MaeII ACGT 2 cut(s) 177, 339
MaeIII GTNAC 6 cut(s) 89, 266, 367, 381, 617, 926
MalI GATC 6 cut(s) 33, 393, 457, 462, 579, 636
MboI GATC 6 cut(s) 31, 391, 455, 460, 577, 634
MboII GAAGA 1 cut(s) 222
MfeI CAATTG 1 cut(s) 52
MflI RGATCY 1 cut(s) 455
MhlI GDGCHC 2 cut(s) 319, 911
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 3 cut(s) 99, 166, 884
MnlI CCTC 9 cut(s) 138, 155, 179, 182, 206, 304, 373, 784, 936
MseI TTAA 5 cut(s) 110, 227, 527, 698, 893
MslI CAYNNNNRTG 3 cut(s) 338, 591, 735
MspI CCGG 3 cut(s) 61, 453, 497
MspR9I CCNGG 3 cut(s) 61, 497, 783
MunI CAATTG 1 cut(s) 52
MvaI CCWGG 1 cut(s) 783
MvnI CGCG 1 cut(s) 272
MwoI GCNNNNNNNGC 1 cut(s) 778
NciI CCSGG 2 cut(s) 61, 497
NdeII GATC 6 cut(s) 31, 391, 455, 460, 577, 634
NlaIII CATG 6 cut(s) 523, 535, 635, 872, 927, 954
NlaIV GGNNCC 5 cut(s) 166, 167, 217, 334, 908
NmuCI GTSAC 4 cut(s) 266, 367, 381, 617
OliI CACNNNNGTG 2 cut(s) 338, 735
PaeR7I CTCGAG 1 cut(s) 940
PagI TCATGA 1 cut(s) 631
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 3 cut(s) 197, 200, 502
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 165
PshBI ATTAAT 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 165
Psp6I CCWGG 1 cut(s) 781
PspGI CCWGG 1 cut(s) 781
PspN4I GGNNCC 5 cut(s) 166, 167, 217, 334, 908
PspPI GGNCC 4 cut(s) 153, 165, 216, 297
PspPPI RGGWCCY 1 cut(s) 165
PsuI RGATCY 1 cut(s) 455
RsaI GTAC 9 cut(s) 125, 149, 304, 448, 562, 677, 747, 853, 949
RsaNI GTAC 9 cut(s) 124, 148, 303, 447, 561, 676, 746, 852, 948
RseI CAYNNNNRTG 3 cut(s) 338, 591, 735
SaqAI TTAA 5 cut(s) 110, 227, 527, 698, 893
SatI GCNGC 3 cut(s) 196, 199, 501
Sau3AI GATC 6 cut(s) 31, 391, 455, 460, 577, 634
Sau96I GGNCC 4 cut(s) 153, 165, 216, 297
ScaI AGTACT 3 cut(s) 304, 448, 562
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 3 cut(s) 61, 497, 783
SduI GDGCHC 2 cut(s) 319, 911
SfaNI GCATC 2 cut(s) 283, 727
SfcI CTRYAG 2 cut(s) 103, 258
Sfr274I CTCGAG 1 cut(s) 940
SinI GGWCC 3 cut(s) 153, 165, 216
SlaI CTCGAG 1 cut(s) 940
SmiMI CAYNNNNRTG 3 cut(s) 338, 591, 735
SmlI CTYRAG 2 cut(s) 372, 940
SmoI CTYRAG 2 cut(s) 372, 940
SpeI ACTAGT 1 cut(s) 557
SsiI CCGC 2 cut(s) 199, 237
SspMI CTAG 2 cut(s) 558, 863
StyD4I CCNGG 3 cut(s) 59, 495, 781
StyI CCWWGG 1 cut(s) 119
TaaI ACNGT 1 cut(s) 671
TaiI ACGT 2 cut(s) 180, 342
TaqI TCGA 2 cut(s) 492, 941
TatI WGTACW 6 cut(s) 302, 446, 560, 675, 745, 947
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 5 cut(s) 110, 227, 527, 698, 893
Tru9I TTAA 5 cut(s) 110, 227, 527, 698, 893
TscAI CASTG 2 cut(s) 372, 652
TseFI GTSAC 4 cut(s) 266, 367, 381, 617
TseI GCWGC 2 cut(s) 195, 500
Tsp45I GTSAC 4 cut(s) 266, 367, 381, 617
TspDTI ATGAA 4 cut(s) 548, 692, 866, 885
TspRI CASTG 2 cut(s) 372, 652
VpaK11BI GGWCC 3 cut(s) 153, 165, 216
VspI ATTAAT 1 cut(s) 698
XapI RAATTY 3 cut(s) 83, 412, 488
XhoI CTCGAG 1 cut(s) 940
XmiI GTMKAC 1 cut(s) 803
XspI CTAG 2 cut(s) 558, 863
ZrmI AGTACT 3 cut(s) 304, 448, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.