MD14G1040400.v1.1

Zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0016059
Physical Location & Seq
Reverse (-)
3791701 .. 3792657
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1040400.v1.1.491

Sequence Viewer

Length: 957 bp
ATGGAGAGAGCAGGATGGAAACTAAACGATGATCATCATCACATATCTCCCAATTGTCCCAGGTGTGGTTGTTCCAACACCAAATTTTGTTACCACAACAACTATAGTTTAACTCAGCCAAGGTACTTTTGCAAGGGCTGTAGGAGGTACTGGACCAAAGGTGGATCCCTCAGGAACGTCCCTGTCGGAGGCGGCTGCCGCAAGAATAGAAGAGGGTCCAAGTCTTTAAGGCTATCCACCACCACCACCACCACCACACAAGGTCACGCACAGCACAACGCTAATAAGAGTAGTGCCAATACTTATGAGGGTGCTCATAATTCCTATGGCACCAACGTGTTGGGTGATTCCACAAGTTCCTCGATGACTCAAGATGGGTCACATATTGATCTTGCACTTGTTTATGCAAATTTCTTGAACCAGAAGCCAGATAATTCAGATAGTACTACTGGATCTGATCATCAACTACCAGATTTGCCTAGCGAATTCAGCCCGGCGGCTTTAGATTTTTCAAGCATGGCGTTGAACATGAACAATGCAAGCTCAGGCAACACTAGTACTCAGTTAATGGGTGATCATCATCATCAAAATGGTCTTGTTGGGTCACTTGGATGTCACACTACTCTATCTCAAGATCACTTATCCACTGAAAATTGTAGGATCAATGACCTCATGTACTTTGGTGGATTAGACCCATTAATTCATCATCCAGAGAAGCATCGTCATCATCAAGATACAAGTGAAGTAGTACAATATGCAAGCGATTTCGGGTTGCCGCCATTGCCAGGCCAAGAGGCATTGTGGTCTACTACTTATCAAATGATGGATAGTGGTAGTCATACAATGCAGGCTCCTACATCGCTATTAGGACCAGAACCTCATGATCCAAACTTGTTAATGGGTGGTTGGAGCCCGTTTGAATTGCCATGTAATGAAACTTTCGCCAGGAGTACATGA

Protein Analysis

319

Amino Acids

34.92

Weight (kDa)

6.38

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_Dof PF02701 18 - 71 6.1e-29 Dof domain, zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52440 AT3G52440
fragaria_vesca FvH4_6g20401
malus_domestica MD12G1041000.v1.1 MD14G1040400.v1.1
prunus_persica Prupe.7G093900_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0476121
rosa_laevigata RLG00000023789
rosa_multiflora Rmu_sc0000114.1_g000027
rosa_roxburghii Rroxscaffold_6G00405460
rosa_rugosa Rorug03G0152900
rosa_samantha Rh3BG234200 Rh3CG229600 Rh3DG229600
rosa_wichuraiana Rw3G018540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 806
AciI CCGC 4 cut(s) 192, 199, 497, 776
AclWI GGATC 5 cut(s) 159, 172, 460, 668, 878
AcsI RAATTY 3 cut(s) 83, 409, 485
AfaI GTAC 7 cut(s) 125, 149, 445, 559, 677, 750, 952
AfiI CCNNNNNNNGG 3 cut(s) 65, 188, 785
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 4 cut(s) 418, 513, 526, 920
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 3 cut(s) 59, 784, 944
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 1 cut(s) 543
AluI AGCT 1 cut(s) 543
Alw21I GWGCWC 1 cut(s) 316
AlwI GGATC 5 cut(s) 159, 172, 460, 668, 878
AoxI GGCC 1 cut(s) 787
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 3 cut(s) 83, 409, 485
AseI ATTAAT 1 cut(s) 698
AspS9I GGNCC 3 cut(s) 153, 216, 869
AsuC2I CCSGG 1 cut(s) 494
AsuHPI GGTGA 2 cut(s) 356, 584
AvaII GGWCC 3 cut(s) 153, 216, 869
AxyI CCTNAGG 1 cut(s) 170
BamHI GGATCC 1 cut(s) 164
BanI GGYRCC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 914
Bbv12I GWGCWC 1 cut(s) 316
BbvI GCAGC 1 cut(s) 182
BccI CCATC 3 cut(s) 9, 368, 817
BciT130I CCWGG 3 cut(s) 61, 786, 946
BclI TGATCA 3 cut(s) 31, 457, 574
BcnI CCSGG 1 cut(s) 494
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 2 cut(s) 480, 555
BfmI CTRYAG 2 cut(s) 103, 139
BisI GCNGC 5 cut(s) 193, 196, 199, 498, 776
BlsI GCNGC 5 cut(s) 194, 197, 200, 499, 777
BmcAI AGTACT 2 cut(s) 445, 559
Bme1390I CCNGG 4 cut(s) 61, 494, 786, 946
Bme18I GGWCC 3 cut(s) 153, 216, 869
BmgT120I GGNCC 3 cut(s) 153, 216, 869
BmiI GGNNCC 5 cut(s) 166, 217, 331, 852, 911
BmrFI CCNGG 4 cut(s) 61, 494, 786, 946
BmsI GCATC 1 cut(s) 727
Bpu10I CCTNAGC 1 cut(s) 544
BpuEI CTTGAG 2 cut(s) 354, 615
BpuMI CCSGG 1 cut(s) 494
BsaBI GATNNNNATC 3 cut(s) 33, 36, 579
BsaJI CCNNGG 2 cut(s) 59, 119
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 188, 785
Bse1I ACTGG 2 cut(s) 155, 454
Bse21I CCTNAGG 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 779
Bse8I GATNNNNATC 3 cut(s) 33, 36, 579
BseBI CCWGG 3 cut(s) 61, 786, 946
BseDI CCNNGG 2 cut(s) 59, 119
BseGI GGATG 3 cut(s) 20, 617, 706
BseJI GATNNNNATC 3 cut(s) 33, 36, 579
BseLI CCNNNNNNNGG 3 cut(s) 65, 188, 785
BseMI GCAATG 1 cut(s) 779
BseMII CTCAG 4 cut(s) 128, 184, 558, 575
BseNI ACTGG 2 cut(s) 155, 454
BseXI GCAGC 1 cut(s) 182
BshFI GGCC 1 cut(s) 789
BshNI GGYRCC 1 cut(s) 329
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 1 cut(s) 494
BslFI GGGAC 2 cut(s) 42, 164
BslI CCNNNNNNNGG 3 cut(s) 65, 188, 785
BsmFI GGGAC 2 cut(s) 42, 164
BsnI GGCC 1 cut(s) 789
Bsp1286I GDGCHC 2 cut(s) 316, 914
Bsp143I GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
BspACI CCGC 4 cut(s) 192, 199, 497, 776
BspANI GGCC 1 cut(s) 789
BspCNI CTCAG 4 cut(s) 127, 183, 557, 574
BspHI TCATGA 1 cut(s) 880
BspLI GGNNCC 5 cut(s) 166, 217, 331, 852, 911
BspPI GGATC 5 cut(s) 159, 172, 460, 668, 878
BspT107I GGYRCC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 779
BsrI ACTGG 2 cut(s) 155, 454
BssECI CCNNGG 2 cut(s) 59, 119
BssMI GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
BssT1I CCWWGG 1 cut(s) 119
Bst2UI CCWGG 3 cut(s) 61, 786, 946
Bst6I CTCTTC 1 cut(s) 205
BstC8I GCNNGC 3 cut(s) 541, 760, 849
BstDEI CTNAG 4 cut(s) 114, 170, 544, 561
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 3 cut(s) 20, 617, 706
BstKTI GATC 9 cut(s) 34, 167, 391, 455, 460, 577, 637, 663, 886
BstMBI GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
BstMWI GCNNNNNNNGC 3 cut(s) 198, 489, 781
BstNI CCWGG 3 cut(s) 61, 786, 946
BstSCI CCNGG 4 cut(s) 59, 492, 784, 944
BstSFI CTRYAG 2 cut(s) 103, 139
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 164, 452
BstXI CCANNNNNNTGG 1 cut(s) 340
BstYI RGATCY 2 cut(s) 164, 452
Bsu36I CCTNAGG 1 cut(s) 170
BsuRI GGCC 1 cut(s) 789
BtgZI GCGATG 1 cut(s) 843
BtsCI GGATG 3 cut(s) 20, 617, 706
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 3 cut(s) 541, 760, 849
CciI TCATGA 1 cut(s) 880
Cfr13I GGNCC 3 cut(s) 153, 216, 869
Csp6I GTAC 7 cut(s) 124, 148, 444, 558, 676, 749, 951
CviAII CATG 6 cut(s) 517, 529, 673, 881, 927, 954
CviQI GTAC 7 cut(s) 124, 148, 444, 558, 676, 749, 951
DdeI CTNAG 4 cut(s) 114, 170, 544, 561
DpnI GATC 9 cut(s) 33, 166, 390, 454, 459, 576, 636, 662, 885
DpnII GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 914
Eco47I GGWCC 3 cut(s) 153, 216, 869
Eco81I CCTNAGG 1 cut(s) 170
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRI GAATTC 1 cut(s) 485
EcoRII CCWGG 3 cut(s) 59, 784, 944
EcoT14I CCWWGG 1 cut(s) 119
EcoT38I GRGCYC 1 cut(s) 914
ErhI CCWWGG 1 cut(s) 119
FaeI CATG 6 cut(s) 520, 532, 676, 884, 930, 957
FaqI GGGAC 2 cut(s) 42, 164
FatI CATG 6 cut(s) 516, 528, 672, 880, 926, 953
FbaI TGATCA 3 cut(s) 31, 457, 574
FblI GTMKAC 1 cut(s) 806
Fnu4HI GCNGC 5 cut(s) 193, 196, 199, 498, 776
FokI GGATG 3 cut(s) 27, 624, 693
FriOI GRGCYC 1 cut(s) 914
Fsp4HI GCNGC 5 cut(s) 193, 196, 199, 498, 776
FspBI CTAG 2 cut(s) 480, 555
GluI GCNGC 5 cut(s) 193, 196, 199, 498, 776
HaeIII GGCC 1 cut(s) 789
HapII CCGG 1 cut(s) 494
Hin1II CATG 6 cut(s) 520, 532, 676, 884, 930, 957
HinfI GANTC 2 cut(s) 347, 367
HpaII CCGG 1 cut(s) 494
HphI GGTGA 2 cut(s) 356, 584
Hpy166II GTNNAC 1 cut(s) 807
Hpy188I TCNGA 3 cut(s) 188, 439, 457
Hpy188III TCNNGA 7 cut(s) 172, 371, 415, 632, 710, 731, 881
Hpy8I GTNNAC 1 cut(s) 807
HpyCH4IV ACGT 2 cut(s) 177, 336
HpyCH4V TGCA 6 cut(s) 132, 395, 407, 539, 758, 847
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 489, 781
HpyF3I CTNAG 4 cut(s) 114, 170, 544, 561
HpySE526I ACGT 2 cut(s) 177, 336
Hsp92II CATG 6 cut(s) 520, 532, 676, 884, 930, 957
Ksp22I TGATCA 3 cut(s) 31, 457, 574
Kzo9I GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
LmnI GCTCC 2 cut(s) 856, 909
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 1 cut(s) 727
MaeI CTAG 2 cut(s) 480, 555
MaeII ACGT 2 cut(s) 177, 336
MaeIII GTNAC 5 cut(s) 89, 263, 378, 603, 614
MalI GATC 9 cut(s) 33, 166, 390, 454, 459, 576, 636, 662, 885
MboI GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
MboII GAAGA 1 cut(s) 222
MfeI CAATTG 1 cut(s) 52
MflI RGATCY 2 cut(s) 164, 452
MhlI GDGCHC 2 cut(s) 316, 914
MluCI AATT 9 cut(s) 52, 83, 319, 409, 433, 485, 652, 699, 920
MlyI GAGTC 1 cut(s) 361
MmeI TCCRAC 3 cut(s) 99, 166, 887
MnlI CCTC 9 cut(s) 138, 179, 182, 206, 301, 370, 680, 787, 888
MseI TTAA 5 cut(s) 110, 227, 566, 698, 896
MslI CAYNNNNRTG 3 cut(s) 335, 588, 610
MspI CCGG 1 cut(s) 494
MspR9I CCNGG 4 cut(s) 61, 494, 786, 946
MunI CAATTG 1 cut(s) 52
MvaI CCWGG 3 cut(s) 61, 786, 946
MwoI GCNNNNNNNGC 3 cut(s) 198, 489, 781
NciI CCSGG 1 cut(s) 494
NdeII GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
NlaIII CATG 6 cut(s) 520, 532, 676, 884, 930, 957
NlaIV GGNNCC 5 cut(s) 166, 217, 331, 852, 911
NmuCI GTSAC 4 cut(s) 263, 378, 603, 614
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 880
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 5 cut(s) 194, 197, 200, 499, 777
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 698
Psp6I CCWGG 3 cut(s) 59, 784, 944
PspGI CCWGG 3 cut(s) 59, 784, 944
PspN4I GGNNCC 5 cut(s) 166, 217, 331, 852, 911
PspPI GGNCC 3 cut(s) 153, 216, 869
PsuI RGATCY 2 cut(s) 164, 452
RsaI GTAC 7 cut(s) 125, 149, 445, 559, 677, 750, 952
RsaNI GTAC 7 cut(s) 124, 148, 444, 558, 676, 749, 951
RseI CAYNNNNRTG 3 cut(s) 335, 588, 610
SaqAI TTAA 5 cut(s) 110, 227, 566, 698, 896
SatI GCNGC 5 cut(s) 193, 196, 199, 498, 776
Sau3AI GATC 9 cut(s) 31, 164, 388, 452, 457, 574, 634, 660, 883
Sau96I GGNCC 3 cut(s) 153, 216, 869
ScaI AGTACT 2 cut(s) 445, 559
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 4 cut(s) 61, 494, 786, 946
SduI GDGCHC 2 cut(s) 316, 914
SfaNI GCATC 1 cut(s) 727
SfcI CTRYAG 2 cut(s) 103, 139
SinI GGWCC 3 cut(s) 153, 216, 869
SmiMI CAYNNNNRTG 3 cut(s) 335, 588, 610
SmlI CTYRAG 2 cut(s) 369, 630
SmoI CTYRAG 2 cut(s) 369, 630
SpeI ACTAGT 1 cut(s) 554
Sse9I AATT 9 cut(s) 52, 83, 319, 409, 433, 485, 652, 699, 920
SsiI CCGC 4 cut(s) 192, 199, 497, 776
SspMI CTAG 2 cut(s) 480, 555
StyD4I CCNGG 4 cut(s) 59, 492, 784, 944
StyI CCWWGG 1 cut(s) 119
TaiI ACGT 2 cut(s) 180, 339
TaqI TCGA 1 cut(s) 362
TasI AATT 9 cut(s) 52, 83, 319, 409, 433, 485, 652, 699, 920
TatI WGTACW 5 cut(s) 443, 557, 675, 748, 950
TauI GCSGC 4 cut(s) 195, 201, 500, 778
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 5 cut(s) 110, 227, 566, 698, 896
Tru9I TTAA 5 cut(s) 110, 227, 566, 698, 896
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 4 cut(s) 263, 378, 603, 614
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 4 cut(s) 263, 378, 603, 614
TspDTI ATGAA 3 cut(s) 545, 692, 948
TspRI CASTG 1 cut(s) 652
VpaK11BI GGWCC 3 cut(s) 153, 216, 869
VspI ATTAAT 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 186
XapI RAATTY 3 cut(s) 83, 409, 485
XmiI GTMKAC 1 cut(s) 806
XspI CTAG 2 cut(s) 480, 555
ZrmI AGTACT 2 cut(s) 445, 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.