Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011633

Physical Location & Seq

Genomic Coordinates Reverse (-)

5134743 .. 5145685

Gene Structure 10943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1046000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1800 bp

ATGGGGAACACATGTGTAGGACCAAATCTTGGCAAGGGTTTCTTTCAATCTTTTACCGCTGCCGTTTGGAAAAACCGGCCGCCGGAAAACAGGCTTCCTCCTCCCAATGAAGGAAATAGTAGCAAAAGTGGCCCAAGTTCAAATCATGATTCATCTAAAGGAGGTAATGAGGGGAATAAAGCTCCTAAAGCTATTGAGATTCCTCCTAATCCGGTTCAAAGCACACCGCCTCCAGCTGTGAAAATCGGTAATGATCCACATCAAAAGGCAGAACCTGAAAAACCTAATACGCCGGAGGTTAAGGAGGAGGTGAGTAAGCCTCCTCAGGATGCTGAGAAATCGAAGAAACCTACTCATATGAAGAGGATTTCAAGCGTTGGACTGCAAGTAGAATCGGTGTTAGGAAGGAAGACTGGGAATATAAAGGATGTTTATAGCTTGGGCAGGAAGCTTGGACAAGGGCAATTTGGGACGACGTTTCTTTGCGTGGAGAAGGAAACGAATAAAGAGTTTGCTTGCAAGTCGATTGCAAAGAGGAAATTGACAACACAAGAAGACGTCGAGGATGTTAGGAGGGAAATTCAGATAATGCATCATTTGGCAGGTCACCCCAATGTGATACAAATTGTCAGCGCTTATGAGGATGCTGTGGCAGTTCATGTTGTAATGGAACTGTGTGCAGGTGGGGAGCTTTTCGATAGAATTATACAAAGGGGGCATTACACAGAGAGAAAGGCAGCTAAGCTTGCAAGGGTTATCATTGGTGTCCTCGAAGCATGCCATTCTTTGGGTGTCATGCATCGGGACTTAAAGCCAGAGAATTTTCTATTTGTCAACCAGGAAGAGGACGCACCCCTTAAGACAATAGATTTTGGGCTCTCAGTGTTCTTTAGGCCAGGAGAAACATTCACTGATGTTGTTGGGAGCCCTTACTATGTGGCACCAGAAGTACTACGGAAGCACTATGGTCAAGAATGTGATGTTTGGAGTGCTGGTGTGATCATTTATATCTTACTCAGTGGAGTGCCCCCTTTCTGGGATGAAACGGAGCAAGGTATATTTGAGCAGGTATTAAAGGGCGAACTTGATTTCATATCAGAACCATGGCCCAACATATCTGAAAGTGCTAAGGATTTGGTTCGGAGAATGCTTGTGAGGGACCCAAAAAAGCGGCTAACAGCCCATGAAGTTCTTTGCCACCCATGGGTTCAGGTAGATGGTGTTGCTCCTGATAAACCTCTTGATTCTGCTGTTCTAACTCGGTTGAAGCAATTCTCGGCCATGAACAAACTCAAGAAAATTGCCATCAGGGTCATTGCAGAAAGTCTGTCTGAAGAGGAAATTGCAGGCCTCAAAGAAATGTTCAGGATGATAGACGCAGATAATAGTGGGCATATCTCCCTTGAGGAACTGAAGAATGGTTTGGAAAGAGTGGGTGCTGACCTCAAGGATTCTGAAATCAGTTGGTTAATGCAAGCAGCAGATGTAGACAACAGTGGTACCATAGACTATGGTGAATTCATAGCAGCAATGCTCCACCTGAACAAGGTCCAGAAGGAGGATCACCTGTTCGCAGCATTCTCGTACTTCGACAGAGATGGAAGTGGGTACATTACTCGAGATGAGCTGCAACAGGCCTGTGAGAAGTTTGGCTTAGAAGATGTTCACTTAGACGATATAATACGTGAAGTTGACCAGGATAATGATGGGCGAATTGATTATAGTGAGTTTGTTGCTATGATGCAAGACACAGGTTTCGGCAAGGGATTACAAAGTAACATGAGTAGAAAGGTAGCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

600

Amino Acids

66.67

Weight (kDa)

5.57

Isoelectric Point (pI)

44.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	145 - 403	5.7e-79	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	147 - 397	7.4e-26	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	451 - 477	2.7e-07	EF hand domain
EF-hand_7	PF13499	451 - 511	1.1e-12	EF-hand domain pair
EF-hand_6	PF13405	451 - 479	2e-06	EF-hand domain
EF-hand_5	PF13202	451 - 473	5e-06	EF hand
EF-hand_7	PF13499	520 - 582	4.2e-16	EF-hand domain pair
EF-hand_1	PF00036	524 - 549	2.5e-06	EF hand domain
EF-hand_6	PF13405	524 - 551	2.4e-07	EF-hand domain
EF-hand_8	PF13833	536 - 583	1.3e-07	EF-hand domain pair
EF-hand_1	PF00036	557 - 583	3.6e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38910
fragaria_vesca	FvH4_6g21020
malus_domestica	MD12G1046000.v1.1 MD14G1044700.v1.1
prunus_persica	Prupe.7G089600_v2.0.a1 Prupe.7G089600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0477401
rosa_laevigata	RLG00000023725
rosa_multiflora	Rmu_ssc0000341.1_g000023
rosa_roxburghii	Rroxscaffold_6G00404200 Rroxscaffold_6G00404680 Rroxscaffold_6G00404690
rosa_rugosa	Rorug03G0160200 Rorug03G0160300 Rorug03G0160400
rosa_samantha	Rh3AG210700 Rh3CG237800 Rh3DG237500
rosa_wichuraiana	Rw3G019090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	671
AatII	GACGTC	1 cut(s)	561
Acc36I	ACCTGC	3 cut(s)	593, 671, 1057
Acc65I	GGTACC	1 cut(s)	1499
AccB1I	GGYRCC	2 cut(s)	940, 1499
AccB7I	CCANNNNNTGG	2 cut(s)	29, 787
AccI	GTMKAC	1 cut(s)	1488
AciI	CCGC	4 cut(s)	57, 80, 227, 1171
AclWI	GGATC	2 cut(s)	248, 1569
AcoI	YGGCCR	2 cut(s)	77, 1278
AcsI	RAATTY	3 cut(s)	579, 820, 1517
AcuI	CTGAAG	2 cut(s)	1353, 1433
AcyI	GRCGYC	1 cut(s)	558
AfaI	GTAC	4 cut(s)	951, 1501, 1586, 1610
AfeI	AGCGCT	1 cut(s)	634
AflII	CTTAAG	1 cut(s)	857
AflIII	ACRYGT	1 cut(s)	11
AgsI	TTSAA	5 cut(s)	47, 141, 218, 372, 1267
AjnI	CCWGG	3 cut(s)	837, 895, 1695
AjuI	GAANNNNNNNTTGG	2 cut(s)	1406, 1438
AloI	GAACNNNNNNTCC	2 cut(s)	1553, 1585
AluBI	AGCT	9 cut(s)	182, 191, 236, 438, 451, 691, 740, 745, 1627
AluI	AGCT	9 cut(s)	182, 191, 236, 438, 451, 691, 740, 745, 1627
AlwI	GGATC	2 cut(s)	248, 1569
AlwNI	CAGNNNCTG	1 cut(s)	275
Ama87I	CYCGRG	1 cut(s)	1617
Aor51HI	AGCGCT	1 cut(s)	634
AoxI	GGCC	7 cut(s)	77, 130, 893, 1106, 1278, 1348, 1635
ApeKI	GCWGC	6 cut(s)	59, 737, 1478, 1526, 1574, 1627
ApoI	RAATTY	3 cut(s)	579, 820, 1517
ArsI	GACNNNNNNTTYG	2 cut(s)	1739, 1771
Asp700I	GAANNNNTTC	2 cut(s)	1271, 1662
Asp718I	GGTACC	1 cut(s)	1499
AspLEI	GCGC	1 cut(s)	635
AspS9I	GGNCC	5 cut(s)	20, 131, 1107, 1159, 1549
AsuHPI	GGTGA	4 cut(s)	322, 599, 1526, 1556
AvaI	CYCGRG	1 cut(s)	1617
AvaII	GGWCC	3 cut(s)	20, 1159, 1549
AxyI	CCTNAGG	1 cut(s)	324
BaeGI	GKGCMC	1 cut(s)	1029
BaeI	ACNNNNGTAYC	2 cut(s)	1483, 1516
BanI	GGYRCC	2 cut(s)	940, 1499
BanII	GRGCYC	2 cut(s)	879, 929
BbsI	GAAGAC	2 cut(s)	416, 561
BbvI	GCAGC	6 cut(s)	46, 749, 1490, 1538, 1586, 1614
BccI	CCATC	4 cut(s)	1211, 1313, 1592, 1700
BceAI	ACGGC	1 cut(s)	47
BcgI	CGANNNNNNTGC	2 cut(s)	1563, 1597
BciT130I	CCWGG	3 cut(s)	839, 897, 1697
BclI	TGATCA	1 cut(s)	999
BfoI	RGCGCY	1 cut(s)	636
BfrI	CTTAAG	1 cut(s)	857
BfuAI	ACCTGC	3 cut(s)	593, 671, 1057
BisI	GCNGC	8 cut(s)	60, 80, 738, 1172, 1479, 1527, 1575, 1628
BlpI	GCTNAGC	1 cut(s)	741
BlsI	GCNGC	8 cut(s)	61, 81, 739, 1173, 1480, 1528, 1576, 1629
BmcAI	AGTACT	1 cut(s)	951
Bme1390I	CCNGG	3 cut(s)	839, 897, 1697
Bme18I	GGWCC	3 cut(s)	20, 1159, 1549
BmeT110I	CYCGRG	1 cut(s)	1617
BmgT120I	GGNCC	5 cut(s)	20, 131, 1107, 1159, 1549
BmiI	GGNNCC	5 cut(s)	926, 942, 1160, 1161, 1501
BmrFI	CCNGG	3 cut(s)	839, 897, 1697
BmrI	ACTGGG	1 cut(s)	423
BmsI	GCATC	5 cut(s)	319, 601, 634, 808, 1731
BmuI	ACTGGG	1 cut(s)	423
BpiI	GAAGAC	2 cut(s)	416, 561
BplI	GAGNNNNNCTC	2 cut(s)	304, 336
BpmI	CTGGAG	1 cut(s)	216
Bpu10I	CCTNAGC	1 cut(s)	1128
Bpu1102I	GCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	3 cut(s)	1277, 1424, 1430
BsaAI	YACGTR	1 cut(s)	1685
BsaBI	GATNNNNATC	1 cut(s)	258
BsaHI	GRCGYC	1 cut(s)	558
BsaJI	CCNNGG	2 cut(s)	1103, 1202
BsaWI	WCCGGW	1 cut(s)	211
BsaXI	ACNNNNNCTCC	4 cut(s)	214, 244, 979, 1009
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	1 cut(s)	418
Bse21I	CCTNAGG	1 cut(s)	324
Bse3DI	GCAATG	2 cut(s)	1314, 1536
Bse8I	GATNNNNATC	1 cut(s)	258
BseBI	CCWGG	3 cut(s)	839, 897, 1697
BseDI	CCNNGG	2 cut(s)	1103, 1202
BseGI	GGATG	6 cut(s)	334, 433, 571, 649, 1045, 1374
BseJI	GATNNNNATC	1 cut(s)	258
BseMI	GCAATG	2 cut(s)	1314, 1536
BseMII	CTCAG	4 cut(s)	324, 338, 894, 1030
BseNI	ACTGG	1 cut(s)	418
BseRI	GAGGAG	3 cut(s)	90, 312, 320
BseSI	GKGCMC	1 cut(s)	1029
BseX3I	CGGCCG	1 cut(s)	77
BseXI	GCAGC	6 cut(s)	46, 749, 1490, 1538, 1586, 1614
BsgI	GTGCAG	1 cut(s)	699
Bsh1285I	CGRYCG	1 cut(s)	80
BshFI	GGCC	7 cut(s)	79, 132, 895, 1108, 1280, 1350, 1637
BshNI	GGYRCC	2 cut(s)	940, 1499
BsiEI	CGRYCG	1 cut(s)	80
BsiHKCI	CYCGRG	1 cut(s)	1617
BsiSI	CCGG	4 cut(s)	76, 83, 212, 293
BslFI	GGGAC	3 cut(s)	484, 818, 1172
BsmFI	GGGAC	3 cut(s)	484, 818, 1172
BsmI	GAATGC	2 cut(s)	1152, 1577
BsnI	GGCC	7 cut(s)	79, 132, 895, 1108, 1280, 1350, 1637
BsoBI	CYCGRG	1 cut(s)	1617
Bsp1286I	GDGCHC	3 cut(s)	879, 929, 1029
Bsp143I	GATC	3 cut(s)	253, 999, 1561
Bsp1720I	GCTNAGC	1 cut(s)	741
Bsp19I	CCATGG	2 cut(s)	1103, 1202
BspACI	CCGC	4 cut(s)	57, 80, 227, 1171
BspANI	GGCC	7 cut(s)	79, 132, 895, 1108, 1280, 1350, 1637
BspCNI	CTCAG	4 cut(s)	325, 337, 893, 1029
BspHI	TCATGA	1 cut(s)	145
BspLI	GGNNCC	5 cut(s)	926, 942, 1160, 1161, 1501
BspMI	ACCTGC	3 cut(s)	593, 671, 1057
BspPI	GGATC	2 cut(s)	248, 1569
BspT107I	GGYRCC	2 cut(s)	940, 1499
BspTI	CTTAAG	1 cut(s)	857
BsrDI	GCAATG	2 cut(s)	1314, 1536
BsrFI	RCCGGY	1 cut(s)	75
BsrI	ACTGG	1 cut(s)	418
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	2 cut(s)	1103, 1202
BssMI	GATC	3 cut(s)	253, 999, 1561
BssNI	GRCGYC	1 cut(s)	558
BssT1I	CCWWGG	2 cut(s)	1103, 1202
Bst2UI	CCWGG	3 cut(s)	839, 897, 1697
Bst4CI	ACNGT	2 cut(s)	675, 1496
Bst6I	CTCTTC	3 cut(s)	356, 837, 1329
BstACI	GRCGYC	1 cut(s)	558
BstAFI	CTTAAG	1 cut(s)	857
BstBAI	YACGTR	1 cut(s)	1685
BstC8I	GCNNGC	5 cut(s)	517, 747, 778, 1348, 1476
BstDEI	CTNAG	8 cut(s)	324, 333, 741, 880, 1016, 1128, 1654, 1669
BstDSI	CCRYGG	2 cut(s)	1103, 1202
BstEII	GGTNACC	1 cut(s)	605
BstENI	CCTNNNNNAGG	1 cut(s)	1544
BstF5I	GGATG	6 cut(s)	334, 433, 571, 649, 1045, 1374
BstH2I	RGCGCY	1 cut(s)	636
BstHHI	GCGC	1 cut(s)	635
BstKTI	GATC	3 cut(s)	256, 1002, 1564
BstMBI	GATC	3 cut(s)	253, 999, 1561
BstMCI	CGRYCG	1 cut(s)	80
BstMWI	GCNNNNNNNGC	3 cut(s)	129, 188, 746
BstNI	CCWGG	3 cut(s)	839, 897, 1697
BstNSI	RCATGY	2 cut(s)	15, 780
BstPI	GGTNACC	1 cut(s)	605
BstSCI	CCNGG	3 cut(s)	837, 895, 1695
BstSLI	GKGCMC	1 cut(s)	1029
BstV1I	GCAGC	6 cut(s)	46, 749, 1490, 1538, 1586, 1614
BstV2I	GAAGAC	2 cut(s)	416, 561
BstZI	CGGCCG	1 cut(s)	77
Bsu36I	CCTNAGG	1 cut(s)	324
BsuRI	GGCC	7 cut(s)	79, 132, 895, 1108, 1280, 1350, 1637
BtgI	CCRYGG	2 cut(s)	1103, 1202
BtsCI	GGATG	6 cut(s)	334, 433, 571, 649, 1045, 1374
BtsIMutI	CAGTG	4 cut(s)	888, 909, 1024, 1501
BveI	ACCTGC	3 cut(s)	593, 671, 1057
Cac8I	GCNNGC	5 cut(s)	517, 747, 778, 1348, 1476
CaiI	CAGNNNCTG	1 cut(s)	275
CciI	TCATGA	1 cut(s)	145
CfoI	GCGC	1 cut(s)	635
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	5 cut(s)	20, 131, 1107, 1159, 1549
CseI	GACGC	2 cut(s)	857, 1385
Csp6I	GTAC	4 cut(s)	950, 1500, 1585, 1609
CviQI	GTAC	4 cut(s)	950, 1500, 1585, 1609
DdeI	CTNAG	8 cut(s)	324, 333, 741, 880, 1016, 1128, 1654, 1669
DpnI	GATC	3 cut(s)	255, 1001, 1563
DpnII	GATC	3 cut(s)	253, 999, 1561
EaeI	YGGCCR	2 cut(s)	77, 1278
EagI	CGGCCG	1 cut(s)	77
Eam1104I	CTCTTC	3 cut(s)	356, 837, 1329
EarI	CTCTTC	3 cut(s)	356, 837, 1329
EclXI	CGGCCG	1 cut(s)	77
Eco130I	CCWWGG	2 cut(s)	1103, 1202
Eco147I	AGGCCT	2 cut(s)	1350, 1637
Eco24I	GRGCYC	2 cut(s)	879, 929
Eco47I	GGWCC	3 cut(s)	20, 1159, 1549
Eco47III	AGCGCT	1 cut(s)	634
Eco52I	CGGCCG	1 cut(s)	77
Eco57I	CTGAAG	2 cut(s)	1353, 1433
Eco81I	CCTNAGG	1 cut(s)	324
Eco88I	CYCGRG	1 cut(s)	1617
Eco91I	GGTNACC	1 cut(s)	605
EcoNI	CCTNNNNNAGG	1 cut(s)	1544
EcoO109I	RGGNCCY	1 cut(s)	1159
EcoO65I	GGTNACC	1 cut(s)	605
EcoRI	GAATTC	1 cut(s)	1517
EcoRII	CCWGG	3 cut(s)	837, 895, 1695
EcoT14I	CCWWGG	2 cut(s)	1103, 1202
EcoT22I	ATGCAT	2 cut(s)	594, 801
EcoT38I	GRGCYC	2 cut(s)	879, 929
ErhI	CCWWGG	2 cut(s)	1103, 1202
FalI	AAGNNNNNCTT	4 cut(s)	26, 58, 1637, 1669
FaqI	GGGAC	3 cut(s)	484, 818, 1172
FauNDI	CATATG	1 cut(s)	357
FbaI	TGATCA	1 cut(s)	999
FblI	GTMKAC	1 cut(s)	1488
Fnu4HI	GCNGC	8 cut(s)	60, 80, 738, 1172, 1479, 1527, 1575, 1628
FokI	GGATG	6 cut(s)	341, 440, 578, 656, 1052, 1381
FriOI	GRGCYC	2 cut(s)	879, 929
Fsp4HI	GCNGC	8 cut(s)	60, 80, 738, 1172, 1479, 1527, 1575, 1628
GlaI	GCGC	1 cut(s)	634
GluI	GCNGC	8 cut(s)	60, 80, 738, 1172, 1479, 1527, 1575, 1628
GsuI	CTGGAG	1 cut(s)	216
HaeII	RGCGCY	1 cut(s)	636
HaeIII	GGCC	7 cut(s)	79, 132, 895, 1108, 1280, 1350, 1637
HapII	CCGG	4 cut(s)	76, 83, 212, 293
HgaI	GACGC	2 cut(s)	857, 1385
HhaI	GCGC	1 cut(s)	635
Hin1I	GRCGYC	1 cut(s)	558
Hin6I	GCGC	1 cut(s)	633
HinP1I	GCGC	1 cut(s)	633
HincII	GTYRAC	2 cut(s)	835, 1693
HindII	GTYRAC	2 cut(s)	835, 1693
HindIII	AAGCTT	2 cut(s)	449, 743
HinfI	GANTC	5 cut(s)	149, 199, 392, 1244, 1451
HpaII	CCGG	4 cut(s)	76, 83, 212, 293
HphI	GGTGA	4 cut(s)	322, 599, 1526, 1556
Hpy166II	GTNNAC	4 cut(s)	835, 1489, 1666, 1693
Hpy188I	TCNGA	6 cut(s)	585, 1099, 1120, 1143, 1333, 1456
Hpy8I	GTNNAC	4 cut(s)	835, 1489, 1666, 1693
Hpy99I	CGWCG	2 cut(s)	478, 563
HpyAV	CCTTC	4 cut(s)	104, 399, 487, 1549
HpyCH4III	ACNGT	2 cut(s)	675, 1496
HpyCH4IV	ACGT	3 cut(s)	476, 558, 1684
HpyF10VI	GCNNNNNNNGC	3 cut(s)	129, 188, 746
HpyF3I	CTNAG	8 cut(s)	324, 333, 741, 880, 1016, 1128, 1654, 1669
HpySE526I	ACGT	3 cut(s)	476, 558, 1684
Hsp92I	GRCGYC	1 cut(s)	558
HspAI	GCGC	1 cut(s)	633
KflI	GGGWCCC	1 cut(s)	1159
KpnI	GGTACC	1 cut(s)	1503
Ksp22I	TGATCA	1 cut(s)	999
Kzo9I	GATC	3 cut(s)	253, 999, 1561
LmnI	GCTCC	6 cut(s)	187, 688, 924, 1048, 1231, 1539
Lsp1109I	GCAGC	6 cut(s)	46, 749, 1490, 1538, 1586, 1614
LweI	GCATC	5 cut(s)	319, 601, 634, 808, 1731
MaeII	ACGT	3 cut(s)	476, 558, 1684
MaeIII	GTNAC	2 cut(s)	605, 1775
MalI	GATC	3 cut(s)	255, 1001, 1563
MboI	GATC	3 cut(s)	253, 999, 1561
MboII	GAAGA	8 cut(s)	355, 373, 421, 566, 854, 1346, 1426, 1670
MhlI	GDGCHC	3 cut(s)	879, 929, 1029
MmeI	TCCRAC	1 cut(s)	358
Mph1103I	ATGCAT	2 cut(s)	594, 801
MroXI	GAANNNNTTC	2 cut(s)	1271, 1662
MseI	TTAA	5 cut(s)	300, 809, 858, 1073, 1469
MslI	CAYNNNNRTG	1 cut(s)	612
MspA1I	CMGCKG	2 cut(s)	59, 236
MspCI	CTTAAG	1 cut(s)	857
MspI	CCGG	4 cut(s)	76, 83, 212, 293
MspR9I	CCNGG	3 cut(s)	839, 897, 1697
Mva1269I	GAATGC	2 cut(s)	1152, 1577
MvaI	CCWGG	3 cut(s)	839, 897, 1697
MwoI	GCNNNNNNNGC	3 cut(s)	129, 188, 746
NcoI	CCATGG	2 cut(s)	1103, 1202
NdeI	CATATG	1 cut(s)	357
NdeII	GATC	3 cut(s)	253, 999, 1561
NlaIV	GGNNCC	5 cut(s)	926, 942, 1160, 1161, 1501
NmeAIII	GCCGAG	1 cut(s)	1256
NmuCI	GTSAC	1 cut(s)	605
NsiI	ATGCAT	2 cut(s)	594, 801
NspI	RCATGY	2 cut(s)	15, 780
PaeI	GCATGC	1 cut(s)	780
PaeR7I	CTCGAG	1 cut(s)	1617
PagI	TCATGA	1 cut(s)	145
PaqCI	CACCTGC	1 cut(s)	671
PceI	AGGCCT	2 cut(s)	1350, 1637
PciI	ACATGT	1 cut(s)	11
PctI	GAATGC	2 cut(s)	1152, 1577
PdmI	GAANNNNTTC	2 cut(s)	1271, 1662
PfeI	GAWTC	5 cut(s)	149, 199, 392, 1244, 1451
PflMI	CCANNNNNTGG	2 cut(s)	29, 787
PkrI	GCNGC	8 cut(s)	61, 81, 739, 1173, 1480, 1528, 1576, 1629
Ppu21I	YACGTR	1 cut(s)	1685
PpuMI	RGGWCCY	1 cut(s)	1159
PscI	ACATGT	1 cut(s)	11
Psp5II	RGGWCCY	1 cut(s)	1159
Psp6I	CCWGG	3 cut(s)	837, 895, 1695
PspEI	GGTNACC	1 cut(s)	605
PspGI	CCWGG	3 cut(s)	837, 895, 1695
PspN4I	GGNNCC	5 cut(s)	926, 942, 1160, 1161, 1501
PspPI	GGNCC	5 cut(s)	20, 131, 1107, 1159, 1549
PspPPI	RGGWCCY	1 cut(s)	1159
PstNI	CAGNNNCTG	1 cut(s)	275
PvuII	CAGCTG	1 cut(s)	236
RsaI	GTAC	4 cut(s)	951, 1501, 1586, 1610
RsaNI	GTAC	4 cut(s)	950, 1500, 1585, 1609
RseI	CAYNNNNRTG	1 cut(s)	612
SaqAI	TTAA	5 cut(s)	300, 809, 858, 1073, 1469
SatI	GCNGC	8 cut(s)	60, 80, 738, 1172, 1479, 1527, 1575, 1628
Sau3AI	GATC	3 cut(s)	253, 999, 1561
Sau96I	GGNCC	5 cut(s)	20, 131, 1107, 1159, 1549
ScaI	AGTACT	1 cut(s)	951
ScrFI	CCNGG	3 cut(s)	839, 897, 1697
SduI	GDGCHC	3 cut(s)	879, 929, 1029
SfaNI	GCATC	5 cut(s)	319, 601, 634, 808, 1731
Sfr274I	CTCGAG	1 cut(s)	1617
SinI	GGWCC	3 cut(s)	20, 1159, 1549
SlaI	CTCGAG	1 cut(s)	1617
SmiMI	CAYNNNNRTG	1 cut(s)	612
SmlI	CTYRAG	5 cut(s)	857, 1292, 1403, 1445, 1617
SmoI	CTYRAG	5 cut(s)	857, 1292, 1403, 1445, 1617
SphI	GCATGC	1 cut(s)	780
SseBI	AGGCCT	2 cut(s)	1350, 1637
SsiI	CCGC	4 cut(s)	57, 80, 227, 1171
StuI	AGGCCT	2 cut(s)	1350, 1637
StyD4I	CCNGG	3 cut(s)	837, 895, 1695
StyI	CCWWGG	2 cut(s)	1103, 1202
TaaI	ACNGT	2 cut(s)	675, 1496
TaiI	ACGT	3 cut(s)	479, 561, 1687
TaqI	TCGA	7 cut(s)	341, 524, 561, 696, 771, 1590, 1618
TatI	WGTACW	1 cut(s)	949
TauI	GCSGC	2 cut(s)	82, 1174
TfiI	GAWTC	5 cut(s)	149, 199, 392, 1244, 1451
Tru1I	TTAA	5 cut(s)	300, 809, 858, 1073, 1469
Tru9I	TTAA	5 cut(s)	300, 809, 858, 1073, 1469
TscAI	CASTG	4 cut(s)	888, 916, 1024, 1501
TseFI	GTSAC	1 cut(s)	605
TseI	GCWGC	6 cut(s)	59, 737, 1478, 1526, 1574, 1627
Tsp45I	GTSAC	1 cut(s)	605
TspDTI	ATGAA	9 cut(s)	123, 141, 374, 647, 1056, 1081, 1200, 1298, 1510
TspGWI	ACGGA	2 cut(s)	970, 1061
TspRI	CASTG	4 cut(s)	888, 916, 1024, 1501
Van91I	CCANNNNNTGG	2 cut(s)	29, 787
Vha464I	CTTAAG	1 cut(s)	857
VpaK11BI	GGWCC	3 cut(s)	20, 1159, 1549
XagI	CCTNNNNNAGG	1 cut(s)	1544
XapI	RAATTY	3 cut(s)	579, 820, 1517
XceI	RCATGY	2 cut(s)	15, 780
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1703
XhoI	CTCGAG	1 cut(s)	1617
XmiI	GTMKAC	1 cut(s)	1488
XmnI	GAANNNNTTC	2 cut(s)	1271, 1662
ZraI	GACGTC	1 cut(s)	559
ZrmI	AGTACT	1 cut(s)	951
Zsp2I	ATGCAT	2 cut(s)	594, 801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.