Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011633

Physical Location & Seq

Genomic Coordinates Reverse (-)

4223192 .. 4228824

Gene Structure 5633 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1044700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGGGAACACATGTGTAGGACCAAATCTTGGTAAGGGTTTCTTTCAATCTTTTACTGCCGCTATTTGGCGAAACCAGCCGCCGGAAAACAGGCTTCCTCCTCCCAATGAAGGAGATAGTATCAAAAGTGGCCCAAGTTCAAATCATGATTCATCTAAAGGAGGTTCTGAAGGGAACAAAGCACTTGATCATCAGGTTCCTGAAGCTATTGAGATTCCTCCTAATCCGGTCCAAAGCACACCGCCTCCAGCTGTGAAAATCGGTAATGATCCACATAAAAAAGCAGAACCTGAAAAACCTAATAAGCCGGAGGTTAAGGAGGAGGTGAGTAAGCCTCCTCAGGATGCTGACAAATCGAAGAAACCTGCTCATATGAAGAGGATTTCGAGCGTTGGACTGCAAGTAGAATCGGTGTTAGGAAGAAAGACTGGCAATATAAAGGATGTTTATAGCTTGGGCAGGAAGCTTGGACAAGGGCAATTTGGGACGACATTTCTCTGTGTGGAGAAGGAAACGAATAAAGAGTTTGCTTGCAAGTCCATTGCAAAGAGGAAATTGACAACACAAGAAGACGTGGAGGATGTTAGGAGGGAAATTCAGATAATGCATCACTTGGCAGGTCACCCCAATGTGATACAAATCATCAGCGCTTATGAGGATGCTGTGGCAGTTCATGTTGTAATGGAACTGTGTGCAGGTGGGGAGCTTTTCGATAGGATTATACAAAGGGGGCATTACACAGAGAGAAAGGCAGCTAAGCTTGCAAGGGTTATCGCTGGTGTCCTCGAAGCATGCCATTCTTTGGGTGTCATGCATCGGGACTTAAAACCAGAGAATTTTCTATTTGTCAACCAGAAAGAGGATGCACCACTTAAGACAATAGATTTTGGGCTCTCTGTGTTCTTTAGGCCAGGAGAAACATTCACTGATGTTGTTGGGAGCCCTTACTATGTGGCGCCAGAAGTACTACGGAAGCACTATGGTCTACAATGTGATGTTTGGAGTGCTGGTGTGATCATTTATATCTTACTCAGTGGAGTGCCCCCTTTCTGGGATGAAACGGAGCACGGTATATTTGAGCAGGTATTAAAAGGCGAACTCGATTTCATATCAGAACCGTGGCCCAGCATATCTGAAAGCGCTAAGGATTTGGTTCGGAGAATGCTTGTGAGGGACCCAAAAAAGCGGCTAACGGCCCATGAAGTTCTTTGCCACCCATGGGTTCAGATAGATGGCGCTGCTCCTGATAAACCTCTTGATTCTGCTGTCCTAACTCGCTTGAAGCAATTCTCGGCCATGAATAAACTCAAGAAAATTGCCATCAGGGTCATTGCTGAAAGCCTGTCTGAAGAGGAAATTGCAGGCCTGAAAGAAATGTTCAGGATGATAGACGCAGATAATAGTGGGCATATCACCCTTGAGGAACTGAAGAATGGTTTGGAAAGAGTGGGTGCTGACGTCAAGGATTCTGAAATCAGTTGGTTAATGCAAGCAGCGGATGTGGACAACAGTGGTACCATAGACTACGGTGAATTCATAGCAGCAATGCTCCACCTGAACAAGGTCCAGAAGGAGGATCACCTGTATGCAGCATTCTCGTACTTTGACACAGATGGAAGTGGGCACATTACTCGAGATGAGCTGCAACAGGCCTGTGAGAAGTTTGGCCTAGAAGATGTTCACTTGGATGATATAATACGTGAAGTTGACCAGGATAATGACGGGCGAATTGATTATAGCGAGTTTGTTGCTATGATGCAAGATACAGGTTTCGGCAAGGGGTTACAAAGTAACATGAGTAGAAAGGTAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

605

Amino Acids

67.12

Weight (kDa)

5.62

Isoelectric Point (pI)

44.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	150 - 408	4.8e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	152 - 402	1.4e-26	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	456 - 482	2.9e-07	EF hand domain
EF-hand_7	PF13499	456 - 516	2.2e-12	EF-hand domain pair
EF-hand_6	PF13405	456 - 484	1.8e-06	EF-hand domain
EF-hand_7	PF13499	525 - 587	5.8e-16	EF-hand domain pair
EF-hand_6	PF13405	528 - 556	3.2e-07	EF-hand domain
EF-hand_1	PF00036	529 - 554	5.4e-06	EF hand domain
EF-hand_8	PF13833	543 - 588	1e-06	EF-hand domain pair
EF-hand_1	PF00036	562 - 588	3.6e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38910
fragaria_vesca	FvH4_6g21020
malus_domestica	MD12G1046000.v1.1 MD14G1044700.v1.1
prunus_persica	Prupe.7G089600_v2.0.a1 Prupe.7G089600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0477401
rosa_laevigata	RLG00000023725
rosa_multiflora	Rmu_ssc0000341.1_g000023
rosa_roxburghii	Rroxscaffold_6G00404200 Rroxscaffold_6G00404680 Rroxscaffold_6G00404690
rosa_rugosa	Rorug03G0160200 Rorug03G0160300 Rorug03G0160400
rosa_samantha	Rh3AG210700 Rh3CG237800 Rh3DG237500
rosa_wichuraiana	Rw3G019090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	686
AatII	GACGTC	1 cut(s)	1461
Acc36I	ACCTGC	4 cut(s)	373, 608, 686, 1072
Acc65I	GGTACC	1 cut(s)	1514
AccB1I	GGYRCC	2 cut(s)	955, 1514
AccB7I	CCANNNNNTGG	2 cut(s)	29, 802
AccI	GTMKAC	1 cut(s)	985
AciI	CCGC	5 cut(s)	60, 80, 242, 1186, 1496
AclWI	GGATC	2 cut(s)	263, 1584
AcoI	YGGCCR	1 cut(s)	1293
AcsI	RAATTY	3 cut(s)	594, 835, 1532
AcuI	CTGAAG	4 cut(s)	189, 222, 1368, 1448
AcyI	GRCGYC	2 cut(s)	956, 1458
AfaI	GTAC	3 cut(s)	966, 1516, 1601
AfeI	AGCGCT	2 cut(s)	649, 1141
AflII	CTTAAG	1 cut(s)	872
AflIII	ACRYGT	1 cut(s)	11
AgsI	TTSAA	3 cut(s)	47, 141, 1282
AjiI	CACGTC	1 cut(s)	574
AjnI	CCWGG	2 cut(s)	910, 1710
AjuI	GAANNNNNNNTTGG	2 cut(s)	1421, 1453
AluBI	AGCT	8 cut(s)	206, 251, 453, 466, 706, 755, 760, 1642
AluI	AGCT	8 cut(s)	206, 251, 453, 466, 706, 755, 760, 1642
Alw21I	GWGCWC	1 cut(s)	1068
AlwI	GGATC	2 cut(s)	263, 1584
AlwNI	CAGNNNCTG	1 cut(s)	290
Ama87I	CYCGRG	1 cut(s)	1632
Aor51HI	AGCGCT	2 cut(s)	649, 1141
AoxI	GGCC	8 cut(s)	130, 908, 1121, 1194, 1293, 1363, 1650, 1666
ApeKI	GCWGC	6 cut(s)	752, 1238, 1493, 1541, 1589, 1642
ApoI	RAATTY	3 cut(s)	594, 835, 1532
Asp700I	GAANNNNTTC	2 cut(s)	1286, 1677
Asp718I	GGTACC	1 cut(s)	1514
AspLEI	GCGC	4 cut(s)	650, 958, 1142, 1238
AspS9I	GGNCC	7 cut(s)	20, 131, 229, 1122, 1174, 1195, 1564
AsuHPI	GGTGA	5 cut(s)	337, 614, 1405, 1541, 1571
AvaI	CYCGRG	1 cut(s)	1632
AvaII	GGWCC	4 cut(s)	20, 229, 1174, 1564
AxyI	CCTNAGG	1 cut(s)	339
BaeGI	GKGCMC	2 cut(s)	1044, 1626
BaeI	ACNNNNGTAYC	2 cut(s)	1498, 1531
BanI	GGYRCC	2 cut(s)	955, 1514
BanII	GRGCYC	2 cut(s)	894, 944
BbsI	GAAGAC	1 cut(s)	576
Bbv12I	GWGCWC	1 cut(s)	1068
BbvI	GCAGC	6 cut(s)	764, 1225, 1505, 1553, 1601, 1629
BccI	CCATC	3 cut(s)	1226, 1328, 1607
BceAI	ACGGC	1 cut(s)	1209
BcgI	CGANNNNNNTGC	6 cut(s)	1578, 1612, 1613, 1647, 1730, 1764
BciT130I	CCWGG	2 cut(s)	912, 1712
BclI	TGATCA	2 cut(s)	187, 1014
BfaI	CTAG	1 cut(s)	1670
BfoI	RGCGCY	4 cut(s)	651, 959, 1143, 1239
BfrI	CTTAAG	1 cut(s)	872
BfuAI	ACCTGC	4 cut(s)	373, 608, 686, 1072
BisI	GCNGC	9 cut(s)	60, 80, 753, 1187, 1239, 1494, 1542, 1590, 1643
BlpI	GCTNAGC	1 cut(s)	756
BlsI	GCNGC	9 cut(s)	61, 81, 754, 1188, 1240, 1495, 1543, 1591, 1644
BmcAI	AGTACT	1 cut(s)	966
Bme1390I	CCNGG	2 cut(s)	912, 1712
Bme18I	GGWCC	4 cut(s)	20, 229, 1174, 1564
BmeT110I	CYCGRG	1 cut(s)	1632
BmgBI	CACGTC	1 cut(s)	574
BmgT120I	GGNCC	7 cut(s)	20, 131, 229, 1122, 1174, 1195, 1564
BmiI	GGNNCC	6 cut(s)	198, 941, 957, 1175, 1176, 1516
BmrFI	CCNGG	2 cut(s)	912, 1712
BmsI	GCATC	6 cut(s)	334, 616, 649, 823, 853, 1746
BpiI	GAAGAC	1 cut(s)	576
BplI	GAGNNNNNCTC	2 cut(s)	319, 351
BpmI	CTGGAG	1 cut(s)	231
Bpu10I	CCTNAGC	1 cut(s)	1143
Bpu1102I	GCTNAGC	1 cut(s)	756
BpuEI	CTTGAG	2 cut(s)	1292, 1439
BsaAI	YACGTR	1 cut(s)	1700
BsaBI	GATNNNNATC	1 cut(s)	638
BsaHI	GRCGYC	2 cut(s)	956, 1458
BsaJI	CCNNGG	2 cut(s)	1118, 1217
BsaWI	WCCGGW	1 cut(s)	226
BsaXI	ACNNNNNCTCC	4 cut(s)	229, 259, 994, 1024
Bse1I	ACTGG	1 cut(s)	433
Bse21I	CCTNAGG	1 cut(s)	339
Bse3DI	GCAATG	3 cut(s)	540, 1329, 1551
Bse8I	GATNNNNATC	1 cut(s)	638
BseBI	CCWGG	2 cut(s)	912, 1712
BseDI	CCNNGG	2 cut(s)	1118, 1217
BseGI	GGATG	9 cut(s)	349, 448, 586, 664, 868, 1060, 1389, 1504, 1693
BseJI	GATNNNNATC	1 cut(s)	638
BseMI	GCAATG	3 cut(s)	540, 1329, 1551
BseMII	CTCAG	2 cut(s)	353, 1045
BseNI	ACTGG	1 cut(s)	433
BseRI	GAGGAG	3 cut(s)	90, 327, 335
BseSI	GKGCMC	2 cut(s)	1044, 1626
BseXI	GCAGC	6 cut(s)	764, 1225, 1505, 1553, 1601, 1629
BseYI	CCCAGC	1 cut(s)	1124
BsgI	GTGCAG	1 cut(s)	714
BshFI	GGCC	8 cut(s)	132, 910, 1123, 1196, 1295, 1365, 1652, 1668
BshNI	GGYRCC	2 cut(s)	955, 1514
BsiHKAI	GWGCWC	1 cut(s)	1068
BsiHKCI	CYCGRG	1 cut(s)	1632
BsiSI	CCGG	3 cut(s)	83, 227, 308
BslFI	GGGAC	3 cut(s)	499, 833, 1187
BsmFI	GGGAC	3 cut(s)	499, 833, 1187
BsmI	GAATGC	2 cut(s)	1167, 1592
BsnI	GGCC	8 cut(s)	132, 910, 1123, 1196, 1295, 1365, 1652, 1668
BsoBI	CYCGRG	1 cut(s)	1632
Bsp1286I	GDGCHC	5 cut(s)	894, 944, 1044, 1068, 1626
Bsp143I	GATC	4 cut(s)	187, 268, 1014, 1576
Bsp1720I	GCTNAGC	1 cut(s)	756
Bsp19I	CCATGG	1 cut(s)	1217
BspACI	CCGC	5 cut(s)	60, 80, 242, 1186, 1496
BspANI	GGCC	8 cut(s)	132, 910, 1123, 1196, 1295, 1365, 1652, 1668
BspCNI	CTCAG	2 cut(s)	352, 1044
BspHI	TCATGA	1 cut(s)	145
BspLI	GGNNCC	6 cut(s)	198, 941, 957, 1175, 1176, 1516
BspMI	ACCTGC	4 cut(s)	373, 608, 686, 1072
BspPI	GGATC	2 cut(s)	263, 1584
BspT107I	GGYRCC	2 cut(s)	955, 1514
BspTI	CTTAAG	1 cut(s)	872
BsrDI	GCAATG	3 cut(s)	540, 1329, 1551
BsrI	ACTGG	1 cut(s)	433
BssECI	CCNNGG	2 cut(s)	1118, 1217
BssMI	GATC	4 cut(s)	187, 268, 1014, 1576
BssNI	GRCGYC	2 cut(s)	956, 1458
BssT1I	CCWWGG	1 cut(s)	1217
Bst2UI	CCWGG	2 cut(s)	912, 1712
Bst4CI	ACNGT	5 cut(s)	690, 1070, 1119, 1511, 1529
Bst6I	CTCTTC	2 cut(s)	371, 1344
BstACI	GRCGYC	2 cut(s)	956, 1458
BstAFI	CTTAAG	1 cut(s)	872
BstBAI	YACGTR	1 cut(s)	1700
BstC8I	GCNNGC	5 cut(s)	532, 762, 793, 1363, 1491
BstDEI	CTNAG	4 cut(s)	339, 756, 1031, 1143
BstDSI	CCRYGG	2 cut(s)	1118, 1217
BstEII	GGTNACC	1 cut(s)	620
BstENI	CCTNNNNNAGG	1 cut(s)	1559
BstF5I	GGATG	9 cut(s)	349, 448, 586, 664, 868, 1060, 1389, 1504, 1693
BstH2I	RGCGCY	4 cut(s)	651, 959, 1143, 1239
BstHHI	GCGC	4 cut(s)	650, 958, 1142, 1238
BstKTI	GATC	4 cut(s)	190, 271, 1017, 1579
BstMBI	GATC	4 cut(s)	187, 268, 1014, 1576
BstMWI	GCNNNNNNNGC	2 cut(s)	76, 761
BstNI	CCWGG	2 cut(s)	912, 1712
BstNSI	RCATGY	2 cut(s)	15, 795
BstPI	GGTNACC	1 cut(s)	620
BstSCI	CCNGG	2 cut(s)	910, 1710
BstSLI	GKGCMC	2 cut(s)	1044, 1626
BstV1I	GCAGC	6 cut(s)	764, 1225, 1505, 1553, 1601, 1629
BstV2I	GAAGAC	1 cut(s)	576
Bsu36I	CCTNAGG	1 cut(s)	339
BsuRI	GGCC	8 cut(s)	132, 910, 1123, 1196, 1295, 1365, 1652, 1668
BtgI	CCRYGG	2 cut(s)	1118, 1217
BtrI	CACGTC	1 cut(s)	574
BtsCI	GGATG	9 cut(s)	349, 448, 586, 664, 868, 1060, 1389, 1504, 1693
BtsIMutI	CAGTG	3 cut(s)	924, 1039, 1516
BveI	ACCTGC	4 cut(s)	373, 608, 686, 1072
Cac8I	GCNNGC	5 cut(s)	532, 762, 793, 1363, 1491
CaiI	CAGNNNCTG	1 cut(s)	290
CciI	TCATGA	1 cut(s)	145
CfoI	GCGC	4 cut(s)	650, 958, 1142, 1238
Cfr13I	GGNCC	7 cut(s)	20, 131, 229, 1122, 1174, 1195, 1564
CseI	GACGC	1 cut(s)	1400
Csp6I	GTAC	3 cut(s)	965, 1515, 1600
CviQI	GTAC	3 cut(s)	965, 1515, 1600
DdeI	CTNAG	4 cut(s)	339, 756, 1031, 1143
DinI	GGCGCC	1 cut(s)	957
DpnI	GATC	4 cut(s)	189, 270, 1016, 1578
DpnII	GATC	4 cut(s)	187, 268, 1014, 1576
EaeI	YGGCCR	1 cut(s)	1293
Eam1104I	CTCTTC	2 cut(s)	371, 1344
EarI	CTCTTC	2 cut(s)	371, 1344
Eco130I	CCWWGG	1 cut(s)	1217
Eco147I	AGGCCT	2 cut(s)	1365, 1652
Eco24I	GRGCYC	2 cut(s)	894, 944
Eco47I	GGWCC	4 cut(s)	20, 229, 1174, 1564
Eco47III	AGCGCT	2 cut(s)	649, 1141
Eco57I	CTGAAG	4 cut(s)	189, 222, 1368, 1448
Eco81I	CCTNAGG	1 cut(s)	339
Eco88I	CYCGRG	1 cut(s)	1632
Eco91I	GGTNACC	1 cut(s)	620
EcoNI	CCTNNNNNAGG	1 cut(s)	1559
EcoO109I	RGGNCCY	1 cut(s)	1174
EcoO65I	GGTNACC	1 cut(s)	620
EcoRI	GAATTC	1 cut(s)	1532
EcoRII	CCWGG	2 cut(s)	910, 1710
EcoT14I	CCWWGG	1 cut(s)	1217
EcoT22I	ATGCAT	2 cut(s)	609, 816
EcoT38I	GRGCYC	2 cut(s)	894, 944
EgeI	GGCGCC	1 cut(s)	957
EheI	GGCGCC	1 cut(s)	957
ErhI	CCWWGG	1 cut(s)	1217
FalI	AAGNNNNNCTT	2 cut(s)	26, 58
FaqI	GGGAC	3 cut(s)	499, 833, 1187
FauNDI	CATATG	1 cut(s)	372
FbaI	TGATCA	2 cut(s)	187, 1014
FblI	GTMKAC	1 cut(s)	985
Fnu4HI	GCNGC	9 cut(s)	60, 80, 753, 1187, 1239, 1494, 1542, 1590, 1643
FokI	GGATG	9 cut(s)	356, 455, 593, 671, 875, 1067, 1396, 1511, 1700
FriOI	GRGCYC	2 cut(s)	894, 944
Fsp4HI	GCNGC	9 cut(s)	60, 80, 753, 1187, 1239, 1494, 1542, 1590, 1643
FspBI	CTAG	1 cut(s)	1670
GlaI	GCGC	4 cut(s)	649, 957, 1141, 1237
GluI	GCNGC	9 cut(s)	60, 80, 753, 1187, 1239, 1494, 1542, 1590, 1643
GsaI	CCCAGC	1 cut(s)	1128
GsuI	CTGGAG	1 cut(s)	231
HaeII	RGCGCY	4 cut(s)	651, 959, 1143, 1239
HaeIII	GGCC	8 cut(s)	132, 910, 1123, 1196, 1295, 1365, 1652, 1668
HapII	CCGG	3 cut(s)	83, 227, 308
HgaI	GACGC	1 cut(s)	1400
HhaI	GCGC	4 cut(s)	650, 958, 1142, 1238
Hin1I	GRCGYC	2 cut(s)	956, 1458
Hin6I	GCGC	4 cut(s)	648, 956, 1140, 1236
HinP1I	GCGC	4 cut(s)	648, 956, 1140, 1236
HincII	GTYRAC	2 cut(s)	850, 1708
HindII	GTYRAC	2 cut(s)	850, 1708
HindIII	AAGCTT	2 cut(s)	464, 758
HinfI	GANTC	5 cut(s)	149, 214, 407, 1259, 1466
HpaII	CCGG	3 cut(s)	83, 227, 308
HphI	GGTGA	5 cut(s)	337, 614, 1405, 1541, 1571
Hpy166II	GTNNAC	5 cut(s)	850, 986, 1504, 1681, 1708
Hpy188I	TCNGA	8 cut(s)	169, 600, 1114, 1135, 1158, 1227, 1348, 1471
Hpy8I	GTNNAC	5 cut(s)	850, 986, 1504, 1681, 1708
HpyAV	CCTTC	4 cut(s)	104, 164, 502, 1564
HpyCH4III	ACNGT	5 cut(s)	690, 1070, 1119, 1511, 1529
HpyCH4IV	ACGT	3 cut(s)	573, 1458, 1699
HpyF10VI	GCNNNNNNNGC	2 cut(s)	76, 761
HpyF3I	CTNAG	4 cut(s)	339, 756, 1031, 1143
HpySE526I	ACGT	3 cut(s)	573, 1458, 1699
Hsp92I	GRCGYC	2 cut(s)	956, 1458
HspAI	GCGC	4 cut(s)	648, 956, 1140, 1236
KasI	GGCGCC	1 cut(s)	955
KflI	GGGWCCC	1 cut(s)	1174
KpnI	GGTACC	1 cut(s)	1518
Ksp22I	TGATCA	2 cut(s)	187, 1014
Kzo9I	GATC	4 cut(s)	187, 268, 1014, 1576
LmnI	GCTCC	5 cut(s)	703, 939, 1063, 1246, 1554
Lsp1109I	GCAGC	6 cut(s)	764, 1225, 1505, 1553, 1601, 1629
LweI	GCATC	6 cut(s)	334, 616, 649, 823, 853, 1746
MaeI	CTAG	1 cut(s)	1670
MaeII	ACGT	3 cut(s)	573, 1458, 1699
MaeIII	GTNAC	3 cut(s)	620, 1782, 1790
MalI	GATC	4 cut(s)	189, 270, 1016, 1578
MboI	GATC	4 cut(s)	187, 268, 1014, 1576
MboII	GAAGA	7 cut(s)	370, 388, 432, 581, 1361, 1441, 1685
MhlI	GDGCHC	5 cut(s)	894, 944, 1044, 1068, 1626
MluCI	AATT	9 cut(s)	479, 554, 594, 835, 1286, 1314, 1356, 1532, 1728
Mly113I	GGCGCC	1 cut(s)	956
MmeI	TCCRAC	1 cut(s)	373
Mph1103I	ATGCAT	2 cut(s)	609, 816
MroXI	GAANNNNTTC	2 cut(s)	1286, 1677
MseI	TTAA	5 cut(s)	315, 824, 873, 1088, 1484
MslI	CAYNNNNRTG	3 cut(s)	627, 1584, 1686
MspA1I	CMGCKG	2 cut(s)	251, 1496
MspCI	CTTAAG	1 cut(s)	872
MspI	CCGG	3 cut(s)	83, 227, 308
MspR9I	CCNGG	2 cut(s)	912, 1712
Mva1269I	GAATGC	2 cut(s)	1167, 1592
MvaI	CCWGG	2 cut(s)	912, 1712
MwoI	GCNNNNNNNGC	2 cut(s)	76, 761
NarI	GGCGCC	1 cut(s)	956
NcoI	CCATGG	1 cut(s)	1217
NdeI	CATATG	1 cut(s)	372
NdeII	GATC	4 cut(s)	187, 268, 1014, 1576
NlaIV	GGNNCC	6 cut(s)	198, 941, 957, 1175, 1176, 1516
NmeAIII	GCCGAG	1 cut(s)	1271
NmuCI	GTSAC	1 cut(s)	620
NsiI	ATGCAT	2 cut(s)	609, 816
NspI	RCATGY	2 cut(s)	15, 795
PaeI	GCATGC	1 cut(s)	795
PaeR7I	CTCGAG	1 cut(s)	1632
PagI	TCATGA	1 cut(s)	145
PaqCI	CACCTGC	1 cut(s)	686
PceI	AGGCCT	2 cut(s)	1365, 1652
PciI	ACATGT	1 cut(s)	11
PctI	GAATGC	2 cut(s)	1167, 1592
PdmI	GAANNNNTTC	2 cut(s)	1286, 1677
PfeI	GAWTC	5 cut(s)	149, 214, 407, 1259, 1466
PflMI	CCANNNNNTGG	2 cut(s)	29, 802
PkrI	GCNGC	9 cut(s)	61, 81, 754, 1188, 1240, 1495, 1543, 1591, 1644
PluTI	GGCGCC	1 cut(s)	959
Ppu21I	YACGTR	1 cut(s)	1700
PpuMI	RGGWCCY	1 cut(s)	1174
PscI	ACATGT	1 cut(s)	11
Psp5II	RGGWCCY	1 cut(s)	1174
Psp6I	CCWGG	2 cut(s)	910, 1710
PspEI	GGTNACC	1 cut(s)	620
PspFI	CCCAGC	1 cut(s)	1124
PspGI	CCWGG	2 cut(s)	910, 1710
PspN4I	GGNNCC	6 cut(s)	198, 941, 957, 1175, 1176, 1516
PspPI	GGNCC	7 cut(s)	20, 131, 229, 1122, 1174, 1195, 1564
PspPPI	RGGWCCY	1 cut(s)	1174
PstNI	CAGNNNCTG	1 cut(s)	290
PvuII	CAGCTG	1 cut(s)	251
RsaI	GTAC	3 cut(s)	966, 1516, 1601
RsaNI	GTAC	3 cut(s)	965, 1515, 1600
RseI	CAYNNNNRTG	3 cut(s)	627, 1584, 1686
SaqAI	TTAA	5 cut(s)	315, 824, 873, 1088, 1484
SatI	GCNGC	9 cut(s)	60, 80, 753, 1187, 1239, 1494, 1542, 1590, 1643
Sau3AI	GATC	4 cut(s)	187, 268, 1014, 1576
Sau96I	GGNCC	7 cut(s)	20, 131, 229, 1122, 1174, 1195, 1564
ScaI	AGTACT	1 cut(s)	966
ScrFI	CCNGG	2 cut(s)	912, 1712
SduI	GDGCHC	5 cut(s)	894, 944, 1044, 1068, 1626
SfaNI	GCATC	6 cut(s)	334, 616, 649, 823, 853, 1746
SfoI	GGCGCC	1 cut(s)	957
Sfr274I	CTCGAG	1 cut(s)	1632
SinI	GGWCC	4 cut(s)	20, 229, 1174, 1564
SlaI	CTCGAG	1 cut(s)	1632
SmiMI	CAYNNNNRTG	3 cut(s)	627, 1584, 1686
SmlI	CTYRAG	4 cut(s)	872, 1307, 1418, 1632
SmoI	CTYRAG	4 cut(s)	872, 1307, 1418, 1632
SphI	GCATGC	1 cut(s)	795
Sse9I	AATT	9 cut(s)	479, 554, 594, 835, 1286, 1314, 1356, 1532, 1728
SseBI	AGGCCT	2 cut(s)	1365, 1652
SsiI	CCGC	5 cut(s)	60, 80, 242, 1186, 1496
SspDI	GGCGCC	1 cut(s)	955
SspMI	CTAG	1 cut(s)	1670
StuI	AGGCCT	2 cut(s)	1365, 1652
StyD4I	CCNGG	2 cut(s)	910, 1710
StyI	CCWWGG	1 cut(s)	1217
TaaI	ACNGT	5 cut(s)	690, 1070, 1119, 1511, 1529
TaiI	ACGT	3 cut(s)	576, 1461, 1702
TaqI	TCGA	6 cut(s)	356, 386, 711, 786, 1101, 1633
TasI	AATT	9 cut(s)	479, 554, 594, 835, 1286, 1314, 1356, 1532, 1728
TatI	WGTACW	1 cut(s)	964
TauI	GCSGC	3 cut(s)	62, 82, 1189
TfiI	GAWTC	5 cut(s)	149, 214, 407, 1259, 1466
Tru1I	TTAA	5 cut(s)	315, 824, 873, 1088, 1484
Tru9I	TTAA	5 cut(s)	315, 824, 873, 1088, 1484
TscAI	CASTG	3 cut(s)	931, 1039, 1516
TseFI	GTSAC	1 cut(s)	620
TseI	GCWGC	6 cut(s)	752, 1238, 1493, 1541, 1589, 1642
Tsp45I	GTSAC	1 cut(s)	620
TspDTI	ATGAA	9 cut(s)	123, 141, 389, 662, 1071, 1096, 1215, 1313, 1525
TspGWI	ACGGA	2 cut(s)	985, 1076
TspRI	CASTG	3 cut(s)	931, 1039, 1516
Van91I	CCANNNNNTGG	2 cut(s)	29, 802
Vha464I	CTTAAG	1 cut(s)	872
VpaK11BI	GGWCC	4 cut(s)	20, 229, 1174, 1564
XagI	CCTNNNNNAGG	1 cut(s)	1559
XapI	RAATTY	3 cut(s)	594, 835, 1532
XceI	RCATGY	2 cut(s)	15, 795
XhoI	CTCGAG	1 cut(s)	1632
XmiI	GTMKAC	1 cut(s)	985
XmnI	GAANNNNTTC	2 cut(s)	1286, 1677
XspI	CTAG	1 cut(s)	1670
ZraI	GACGTC	1 cut(s)	1459
ZrmI	AGTACT	1 cut(s)	966
Zsp2I	ATGCAT	2 cut(s)	609, 816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.