Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0011633

Physical Location & Seq

Genomic Coordinates Forward (+)

26688421 .. 26693953

Gene Structure 5533 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00404200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1776 bp

ATGGGGAACACATGTGTCGGACCAAATCTTGGCAATGGTTTCATGCAATCCGTTACTGATGCCATTTGGCGAACCCGGCCACCGGAGGACCGGCTTCCTCCTCCAAATGAAGAAGGCAGTAGCAAAAATGACTCAAATTCGAATCCTGGTCCGTCATCCAAAGGTGGCTCTCAGGCTGATCCTAAACCTCCTGATGAGGCTCCTCCTCCAGTCCACAACAATCCCCCTGAGCCTGTGAAAATTGCTAATGATCAACCTCCGAAAGCGGAACCAACAGGAGTGAAGGAAGAGAACAGCAAGGCCCAGGAGGCCGAGAAAGCCAAGAAAGCTGCTCATATGAAGAGGGTTTCTTCTGTTGGACTGCAAATAGACTCGGTGTTAGGAAGGAAGACTGGCAATATCAAGGATCTTTATAGTTTGGGGAGGAAGCTTGGACAAGGACAATTTGGGACTACCTTTCTTTGTGTGGAGAAGAGAACCAATAAGGAGTTTGCATGCAAGTCAATTGCAAAGAGGAAGCTGACTACGCAAGAGGATGTGGAAGATGTTAGGAGGGAAATCCAGATAATGCATCACTTGGCAGGACATCCCAACGTGATACAAATCGTTGGTGCTCATGAGGACGCTGTGGCGGTTCATGTTATAATGGAACTTTGCGCAGGTGGCGAGCTTTTCGATAGGATCATACAAAGAGGGCATTATACGGAGAGAAAAGCAGCTAAACTTGCAAAGATCATAGTTGGTGTTGTTGAAGCATGCCATTCCTTGGGTGTCATGCATCGAGACTTGAAGCCGGAGAACTTTTTATTTGTCAATCAAGAAGAGGAAGCGCCACTTAAGACAATAGATTTTGGGCTCTCAGTGTTCTTTAGGCCAGGAGAAACATTCACTGATGTTGTTGGGAGCCCATATTATGTGGCCCCAGAAGTACTACGGAAGAATTATGGTCAAGCATGTGATGTTTGGAGTGCCGGTGTCATCATCTATATATTACTGAGTGGCGTGCCTCCATTCTGGGATGAAACGGAGCAAGGAATATTTGAGCAGGTTTTAAAAGGTGAACTCGATTTCATATCGGAACCTTGGCCCAACATATCTGAAAGTGCCAAGGATCTGGTCAGGAGAATGCTTATGAGGGACCCCAAAAAGCGGATAACAGCCCATGAAGTACTTCGGCACCCTTGGGTTCAGGTAGATGGTGTTGCTCCTGATAAACCTCTTGATTCTGCTGTCTTAACTCGCTTAAAGCAATTCTCGGCCATGAACAAGCTCAAGAAAATTGCCATCAGAGTAATTGCAGAAAGCCTGTCTGAAGAGGAAATAGCAGGGCTGAAGGAAATGTTCAAGATGATAGACACAGATAATAGTGGGCACATCACCCTTGAGGAACTAAAGACTGGTTTGGAAAGAGTGGGTGCTGATCTCAAGGATTCTGAAATCACTTGGTTAATGCAATCGGCCGATGTAGACAACAGTGGTACCATAGACTATGGTGAGTTCATAGCAGCAATGCTCCACCTGAACAAGGTCCAGAAGGAAGATCACCTGTTTGCAGCATTCTCATACTTTGACCAAGATGGAAGTGGGTACATTACTCGAGATGAGCTGCAACAGGCTTGTGAGAAGTTTGGTTTACAAGATGTGCAATTAGATGACATAATACGTGAAGTCGACCAGGATAATGATGGTCGCATTGATTATAGTGAATTCGTGGCCATGATGGAAGACACCGGTTTTGGCAAGGGATTACAAACAAACATGAGTAGTAGGCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 Pkinase

Protein Analysis

591

Amino Acids

65.71

Weight (kDa)

5.32

Isoelectric Point (pI)

44.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	138 - 396	5.2e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	140 - 391	5.8e-27	Protein tyrosine and serine/threonine kinase
EF-hand_1	PF00036	444 - 469	1.6e-07	EF hand domain
EF-hand_7	PF13499	444 - 504	7.9e-13	EF-hand domain pair
EF-hand_6	PF13405	444 - 471	1e-06	EF-hand domain
EF-hand_5	PF13202	444 - 466	9.6e-06	EF hand
EF-hand_7	PF13499	513 - 574	6.8e-16	EF-hand domain pair
EF-hand_1	PF00036	517 - 542	2.7e-06	EF hand domain
EF-hand_6	PF13405	517 - 544	4e-07	EF-hand domain
EF-hand_8	PF13833	531 - 576	5.5e-07	EF-hand domain pair
EF-hand_1	PF00036	550 - 576	6.5e-08	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011633)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38910
fragaria_vesca	FvH4_6g21020
malus_domestica	MD12G1046000.v1.1 MD14G1044700.v1.1
prunus_persica	Prupe.7G089600_v2.0.a1 Prupe.7G089600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0477401
rosa_laevigata	RLG00000023725
rosa_multiflora	Rmu_ssc0000341.1_g000023
rosa_roxburghii	Rroxscaffold_6G00404200 Rroxscaffold_6G00404680 Rroxscaffold_6G00404690
rosa_rugosa	Rorug03G0160200 Rorug03G0160300 Rorug03G0160400
rosa_samantha	Rh3AG210700 Rh3CG237800 Rh3DG237500
rosa_wichuraiana	Rw3G019090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	644
AarI	CACCTGC	1 cut(s)	650
Acc16I	TGCGCA	1 cut(s)	658
Acc36I	ACCTGC	2 cut(s)	650, 1036
Acc65I	GGTACC	1 cut(s)	1478
AccB1I	GGYRCC	2 cut(s)	1176, 1478
AccB7I	CCANNNNNTGG	2 cut(s)	29, 766
AccI	GTMKAC	2 cut(s)	1467, 1671
AciI	CCGC	3 cut(s)	266, 632, 1150
AclWI	GGATC	4 cut(s)	173, 414, 689, 1119
AcoI	YGGCCR	4 cut(s)	77, 1257, 1458, 1713
AcsI	RAATTY	2 cut(s)	136, 1706
AcuI	CTGAAG	2 cut(s)	1332, 1352
AfaI	GTAC	4 cut(s)	930, 1170, 1480, 1589
AfiI	CCNNNNNNNGG	5 cut(s)	29, 82, 766, 1149, 1525
AflII	CTTAAG	1 cut(s)	836
AflIII	ACRYGT	1 cut(s)	11
AgeI	ACCGGT	1 cut(s)	1730
AgsI	TTSAA	3 cut(s)	752, 790, 1345
AjnI	CCWGG	4 cut(s)	145, 303, 874, 1674
AluBI	AGCT	7 cut(s)	329, 430, 520, 670, 719, 1270, 1606
AluI	AGCT	7 cut(s)	329, 430, 520, 670, 719, 1270, 1606
Alw21I	GWGCWC	1 cut(s)	616
Alw26I	GTCTC	1 cut(s)	777
AlwI	GGATC	4 cut(s)	173, 414, 689, 1119
Ama87I	CYCGRG	1 cut(s)	1596
AoxI	GGCC	9 cut(s)	77, 300, 309, 872, 918, 1085, 1257, 1458, 1713
ApeKI	GCWGC	5 cut(s)	329, 716, 1505, 1553, 1606
ApoI	RAATTY	2 cut(s)	136, 1706
ArsI	GACNNNNNNTTYG	2 cut(s)	1718, 1750
AsiGI	ACCGGT	1 cut(s)	1730
Asp700I	GAANNNNTTC	1 cut(s)	1170
Asp718I	GGTACC	1 cut(s)	1478
AspLEI	GCGC	2 cut(s)	659, 832
AspS9I	GGNCC	8 cut(s)	20, 88, 149, 301, 919, 1086, 1138, 1528
AsuC2I	CCSGG	1 cut(s)	76
AsuHPI	GGTGA	4 cut(s)	1070, 1369, 1505, 1535
AsuII	TTCGAA	1 cut(s)	140
AvaI	CYCGRG	1 cut(s)	1596
AvaII	GGWCC	5 cut(s)	20, 88, 149, 1138, 1528
BaeGI	GKGCMC	1 cut(s)	1374
BaeI	ACNNNNGTAYC	2 cut(s)	1462, 1495
BalI	TGGCCA	1 cut(s)	1715
BanI	GGYRCC	2 cut(s)	1176, 1478
BanII	GRGCYC	2 cut(s)	858, 908
BbsI	GAAGAC	2 cut(s)	395, 1731
Bbv12I	GWGCWC	1 cut(s)	616
BbvI	GCAGC	5 cut(s)	316, 728, 1517, 1565, 1593
BccI	CCATC	5 cut(s)	1190, 1292, 1571, 1679, 1714
BciT130I	CCWGG	4 cut(s)	147, 305, 876, 1676
BclI	TGATCA	1 cut(s)	250
BcnI	CCSGG	1 cut(s)	76
BcoDI	GTCTC	1 cut(s)	777
BfoI	RGCGCY	1 cut(s)	833
BfrI	CTTAAG	1 cut(s)	836
BfuAI	ACCTGC	2 cut(s)	650, 1036
BglI	GCCNNNNNGGC	1 cut(s)	308
BisI	GCNGC	5 cut(s)	330, 717, 1506, 1554, 1607
BlsI	GCNGC	5 cut(s)	331, 718, 1507, 1555, 1608
BmcAI	AGTACT	2 cut(s)	930, 1170
Bme1390I	CCNGG	5 cut(s)	76, 147, 305, 876, 1676
Bme18I	GGWCC	5 cut(s)	20, 88, 149, 1138, 1528
BmeT110I	CYCGRG	1 cut(s)	1596
BmgT120I	GGNCC	8 cut(s)	20, 88, 149, 301, 919, 1086, 1138, 1528
BmiI	GGNNCC	9 cut(s)	201, 270, 905, 921, 1080, 1139, 1140, 1178, 1480
BmrFI	CCNGG	5 cut(s)	76, 147, 305, 876, 1676
BmsI	GCATC	3 cut(s)	49, 580, 787
BpiI	GAAGAC	2 cut(s)	395, 1731
BpmI	CTGGAG	1 cut(s)	192
Bpu10I	CCTNAGC	1 cut(s)	228
Bpu14I	TTCGAA	1 cut(s)	140
BpuEI	CTTGAG	3 cut(s)	1256, 1403, 1409
BpuMI	CCSGG	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	1664
BsaBI	GATNNNNATC	1 cut(s)	602
BsaJI	CCNNGG	5 cut(s)	303, 765, 1082, 1107, 1181
BsaWI	WCCGGW	2 cut(s)	82, 1730
BsaXI	ACNNNNNCTCC	2 cut(s)	958, 988
Bsc4I	CCNNNNNNNGG	5 cut(s)	29, 82, 766, 1149, 1525
Bse118I	RCCGGY	3 cut(s)	90, 971, 1730
Bse1I	ACTGG	3 cut(s)	209, 397, 1402
Bse3DI	GCAATG	2 cut(s)	40, 1515
Bse8I	GATNNNNATC	1 cut(s)	602
BseBI	CCWGG	4 cut(s)	147, 305, 876, 1676
BseDI	CCNNGG	5 cut(s)	303, 765, 1082, 1107, 1181
BseGI	GGATG	4 cut(s)	155, 541, 586, 1024
BseJI	GATNNNNATC	1 cut(s)	602
BseLI	CCNNNNNNNGG	5 cut(s)	29, 82, 766, 1149, 1525
BseMI	GCAATG	2 cut(s)	40, 1515
BseMII	CTCAG	4 cut(s)	185, 219, 873, 986
BseNI	ACTGG	3 cut(s)	209, 397, 1402
BseRI	GAGGAG	3 cut(s)	90, 192, 195
BseSI	GKGCMC	1 cut(s)	1374
BseX3I	CGGCCG	1 cut(s)	1458
BseXI	GCAGC	5 cut(s)	316, 728, 1517, 1565, 1593
Bsh1285I	CGRYCG	1 cut(s)	1461
BshFI	GGCC	9 cut(s)	79, 302, 311, 874, 920, 1087, 1259, 1460, 1715
BshNI	GGYRCC	2 cut(s)	1176, 1478
BshTI	ACCGGT	1 cut(s)	1730
BsiEI	CGRYCG	1 cut(s)	1461
BsiHKAI	GWGCWC	1 cut(s)	616
BsiHKCI	CYCGRG	1 cut(s)	1596
BsiSI	CCGG	6 cut(s)	76, 83, 91, 794, 972, 1731
BslFI	GGGAC	2 cut(s)	463, 1151
BslI	CCNNNNNNNGG	5 cut(s)	29, 82, 766, 1149, 1525
BsmAI	GTCTC	1 cut(s)	777
BsmFI	GGGAC	2 cut(s)	463, 1151
BsmI	GAATGC	2 cut(s)	1131, 1556
BsnI	GGCC	9 cut(s)	79, 302, 311, 874, 920, 1087, 1259, 1460, 1715
BsoBI	CYCGRG	1 cut(s)	1596
Bsp119I	TTCGAA	1 cut(s)	140
Bsp1286I	GDGCHC	4 cut(s)	616, 858, 908, 1374
Bsp143I	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
BspACI	CCGC	3 cut(s)	266, 632, 1150
BspANI	GGCC	9 cut(s)	79, 302, 311, 874, 920, 1087, 1259, 1460, 1715
BspCNI	CTCAG	4 cut(s)	184, 220, 872, 987
BspHI	TCATGA	1 cut(s)	616
BspLI	GGNNCC	9 cut(s)	201, 270, 905, 921, 1080, 1139, 1140, 1178, 1480
BspMI	ACCTGC	2 cut(s)	650, 1036
BspPI	GGATC	4 cut(s)	173, 414, 689, 1119
BspT104I	TTCGAA	1 cut(s)	140
BspT107I	GGYRCC	2 cut(s)	1176, 1478
BspTI	CTTAAG	1 cut(s)	836
BsrDI	GCAATG	2 cut(s)	40, 1515
BsrFI	RCCGGY	3 cut(s)	90, 971, 1730
BsrI	ACTGG	3 cut(s)	209, 397, 1402
BssAI	RCCGGY	3 cut(s)	90, 971, 1730
BssECI	CCNNGG	5 cut(s)	303, 765, 1082, 1107, 1181
BssMI	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
BssT1I	CCWWGG	4 cut(s)	765, 1082, 1107, 1181
Bst2UI	CCWGG	4 cut(s)	147, 305, 876, 1676
Bst4CI	ACNGT	1 cut(s)	1475
Bst6I	CTCTTC	5 cut(s)	282, 335, 467, 816, 1308
BstAFI	CTTAAG	1 cut(s)	836
BstBAI	YACGTR	1 cut(s)	1664
BstBI	TTCGAA	1 cut(s)	140
BstC8I	GCNNGC	4 cut(s)	496, 668, 757, 1004
BstDEI	CTNAG	4 cut(s)	171, 228, 859, 995
BstENI	CCTNNNNNAGG	1 cut(s)	1523
BstF5I	GGATG	4 cut(s)	155, 541, 586, 1024
BstH2I	RGCGCY	1 cut(s)	833
BstHHI	GCGC	2 cut(s)	659, 832
BstKTI	GATC	8 cut(s)	181, 253, 409, 684, 735, 1114, 1423, 1543
BstMAI	GTCTC	1 cut(s)	777
BstMBI	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
BstMCI	CGRYCG	1 cut(s)	1461
BstMWI	GCNNNNNNNGC	7 cut(s)	76, 308, 317, 326, 526, 663, 725
BstNI	CCWGG	4 cut(s)	147, 305, 876, 1676
BstNSI	RCATGY	4 cut(s)	15, 498, 759, 957
BstSCI	CCNGG	5 cut(s)	74, 145, 303, 874, 1674
BstSLI	GKGCMC	1 cut(s)	1374
BstV1I	GCAGC	5 cut(s)	316, 728, 1517, 1565, 1593
BstV2I	GAAGAC	2 cut(s)	395, 1731
BstX2I	RGATCY	2 cut(s)	406, 1111
BstXI	CCANNNNNNTGG	1 cut(s)	1114
BstYI	RGATCY	2 cut(s)	406, 1111
BstZI	CGGCCG	1 cut(s)	1458
BsuRI	GGCC	9 cut(s)	79, 302, 311, 874, 920, 1087, 1259, 1460, 1715
BtsCI	GGATG	4 cut(s)	155, 541, 586, 1024
BtsIMutI	CAGTG	3 cut(s)	867, 888, 1480
BveI	ACCTGC	2 cut(s)	650, 1036
Cac8I	GCNNGC	4 cut(s)	496, 668, 757, 1004
CciI	TCATGA	1 cut(s)	616
CfoI	GCGC	2 cut(s)	659, 832
Cfr10I	RCCGGY	3 cut(s)	90, 971, 1730
Cfr13I	GGNCC	8 cut(s)	20, 88, 149, 301, 919, 1086, 1138, 1528
CseI	GACGC	1 cut(s)	632
Csp6I	GTAC	4 cut(s)	929, 1169, 1479, 1588
CspAI	ACCGGT	1 cut(s)	1730
CviQI	GTAC	4 cut(s)	929, 1169, 1479, 1588
DdeI	CTNAG	4 cut(s)	171, 228, 859, 995
DpnI	GATC	8 cut(s)	180, 252, 408, 683, 734, 1113, 1422, 1542
DpnII	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
DraI	TTTAAA	1 cut(s)	1053
EaeI	YGGCCR	4 cut(s)	77, 1257, 1458, 1713
EagI	CGGCCG	1 cut(s)	1458
Eam1104I	CTCTTC	5 cut(s)	282, 335, 467, 816, 1308
EarI	CTCTTC	5 cut(s)	282, 335, 467, 816, 1308
EclXI	CGGCCG	1 cut(s)	1458
Eco130I	CCWWGG	4 cut(s)	765, 1082, 1107, 1181
Eco24I	GRGCYC	2 cut(s)	858, 908
Eco47I	GGWCC	5 cut(s)	20, 88, 149, 1138, 1528
Eco52I	CGGCCG	1 cut(s)	1458
Eco57I	CTGAAG	2 cut(s)	1332, 1352
Eco88I	CYCGRG	1 cut(s)	1596
EcoNI	CCTNNNNNAGG	1 cut(s)	1523
EcoO109I	RGGNCCY	1 cut(s)	1138
EcoRI	GAATTC	1 cut(s)	1706
EcoRII	CCWGG	4 cut(s)	145, 303, 874, 1674
EcoT14I	CCWWGG	4 cut(s)	765, 1082, 1107, 1181
EcoT22I	ATGCAT	2 cut(s)	573, 780
EcoT38I	GRGCYC	2 cut(s)	858, 908
ErhI	CCWWGG	4 cut(s)	765, 1082, 1107, 1181
FalI	AAGNNNNNCTT	2 cut(s)	819, 851
FaqI	GGGAC	2 cut(s)	463, 1151
FauNDI	CATATG	1 cut(s)	336
FbaI	TGATCA	1 cut(s)	250
FblI	GTMKAC	2 cut(s)	1467, 1671
Fnu4HI	GCNGC	5 cut(s)	330, 717, 1506, 1554, 1607
FokI	GGATG	4 cut(s)	142, 548, 573, 1031
FriOI	GRGCYC	2 cut(s)	858, 908
Fsp4HI	GCNGC	5 cut(s)	330, 717, 1506, 1554, 1607
FspI	TGCGCA	1 cut(s)	658
GlaI	GCGC	2 cut(s)	658, 831
GluI	GCNGC	5 cut(s)	330, 717, 1506, 1554, 1607
GsuI	CTGGAG	1 cut(s)	192
HaeII	RGCGCY	1 cut(s)	833
HaeIII	GGCC	9 cut(s)	79, 302, 311, 874, 920, 1087, 1259, 1460, 1715
HapII	CCGG	6 cut(s)	76, 83, 91, 794, 972, 1731
HgaI	GACGC	1 cut(s)	632
HhaI	GCGC	2 cut(s)	659, 832
Hin6I	GCGC	2 cut(s)	657, 830
HinP1I	GCGC	2 cut(s)	657, 830
HincII	GTYRAC	1 cut(s)	1672
HindII	GTYRAC	1 cut(s)	1672
HindIII	AAGCTT	1 cut(s)	428
HinfI	GANTC	5 cut(s)	131, 142, 371, 1223, 1430
HpaII	CCGG	6 cut(s)	76, 83, 91, 794, 972, 1731
HphI	GGTGA	4 cut(s)	1070, 1369, 1505, 1535
Hpy166II	GTNNAC	5 cut(s)	214, 1061, 1468, 1634, 1672
Hpy188I	TCNGA	7 cut(s)	20, 261, 1078, 1099, 1289, 1312, 1435
Hpy8I	GTNNAC	5 cut(s)	214, 1061, 1468, 1634, 1672
HpyAV	CCTTC	5 cut(s)	107, 277, 378, 1327, 1528
HpyCH4III	ACNGT	1 cut(s)	1475
HpyCH4IV	ACGT	2 cut(s)	594, 1663
HpyF10VI	GCNNNNNNNGC	7 cut(s)	76, 308, 317, 326, 526, 663, 725
HpyF3I	CTNAG	4 cut(s)	171, 228, 859, 995
HpySE526I	ACGT	2 cut(s)	594, 1663
HspAI	GCGC	2 cut(s)	657, 830
KflI	GGGWCCC	1 cut(s)	1138
KpnI	GGTACC	1 cut(s)	1482
Ksp22I	TGATCA	1 cut(s)	250
Kzo9I	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
LmnI	GCTCC	5 cut(s)	205, 903, 1027, 1210, 1518
Lsp1109I	GCAGC	5 cut(s)	316, 728, 1517, 1565, 1593
LweI	GCATC	3 cut(s)	49, 580, 787
MaeII	ACGT	2 cut(s)	594, 1663
MaeIII	GTNAC	1 cut(s)	52
MalI	GATC	8 cut(s)	180, 252, 408, 683, 734, 1113, 1422, 1542
MboI	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
MfeI	CAATTG	1 cut(s)	504
MflI	RGATCY	2 cut(s)	406, 1111
MhlI	GDGCHC	4 cut(s)	616, 858, 908, 1374
MlsI	TGGCCA	1 cut(s)	1715
MluNI	TGGCCA	1 cut(s)	1715
MlyI	GAGTC	2 cut(s)	125, 365
MmeI	TCCRAC	1 cut(s)	337
Mox20I	TGGCCA	1 cut(s)	1715
Mph1103I	ATGCAT	2 cut(s)	573, 780
MroXI	GAANNNNTTC	1 cut(s)	1170
MscI	TGGCCA	1 cut(s)	1715
MseI	TTAA	5 cut(s)	837, 1052, 1235, 1244, 1448
Msp20I	TGGCCA	1 cut(s)	1715
MspCI	CTTAAG	1 cut(s)	836
MspI	CCGG	6 cut(s)	76, 83, 91, 794, 972, 1731
MspR9I	CCNGG	5 cut(s)	76, 147, 305, 876, 1676
MunI	CAATTG	1 cut(s)	504
Mva1269I	GAATGC	2 cut(s)	1131, 1556
MvaI	CCWGG	4 cut(s)	147, 305, 876, 1676
MwoI	GCNNNNNNNGC	7 cut(s)	76, 308, 317, 326, 526, 663, 725
NciI	CCSGG	1 cut(s)	76
NdeI	CATATG	1 cut(s)	336
NdeII	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
NlaIV	GGNNCC	9 cut(s)	201, 270, 905, 921, 1080, 1139, 1140, 1178, 1480
NmeAIII	GCCGAG	2 cut(s)	337, 1235
NsbI	TGCGCA	1 cut(s)	658
NsiI	ATGCAT	2 cut(s)	573, 780
NspI	RCATGY	4 cut(s)	15, 498, 759, 957
NspV	TTCGAA	1 cut(s)	140
PaeI	GCATGC	2 cut(s)	498, 759
PaeR7I	CTCGAG	1 cut(s)	1596
PagI	TCATGA	1 cut(s)	616
PaqCI	CACCTGC	1 cut(s)	650
PciI	ACATGT	1 cut(s)	11
PctI	GAATGC	2 cut(s)	1131, 1556
PdmI	GAANNNNTTC	1 cut(s)	1170
PfeI	GAWTC	3 cut(s)	142, 1223, 1430
PflMI	CCANNNNNTGG	2 cut(s)	29, 766
PinAI	ACCGGT	1 cut(s)	1730
PkrI	GCNGC	5 cut(s)	331, 718, 1507, 1555, 1608
PleI	GAGTC	2 cut(s)	125, 365
PpsI	GAGTC	2 cut(s)	125, 365
Ppu21I	YACGTR	1 cut(s)	1664
PpuMI	RGGWCCY	1 cut(s)	1138
PscI	ACATGT	1 cut(s)	11
PsiI	TTATAA	1 cut(s)	644
Psp5II	RGGWCCY	1 cut(s)	1138
Psp6I	CCWGG	4 cut(s)	145, 303, 874, 1674
PspGI	CCWGG	4 cut(s)	145, 303, 874, 1674
PspN4I	GGNNCC	9 cut(s)	201, 270, 905, 921, 1080, 1139, 1140, 1178, 1480
PspPI	GGNCC	8 cut(s)	20, 88, 149, 301, 919, 1086, 1138, 1528
PspPPI	RGGWCCY	1 cut(s)	1138
PsuI	RGATCY	2 cut(s)	406, 1111
RsaI	GTAC	4 cut(s)	930, 1170, 1480, 1589
RsaNI	GTAC	4 cut(s)	929, 1169, 1479, 1588
SalI	GTCGAC	1 cut(s)	1670
SaqAI	TTAA	5 cut(s)	837, 1052, 1235, 1244, 1448
SatI	GCNGC	5 cut(s)	330, 717, 1506, 1554, 1607
Sau3AI	GATC	8 cut(s)	178, 250, 406, 681, 732, 1111, 1420, 1540
Sau96I	GGNCC	8 cut(s)	20, 88, 149, 301, 919, 1086, 1138, 1528
ScaI	AGTACT	2 cut(s)	930, 1170
SchI	GAGTC	2 cut(s)	125, 365
ScrFI	CCNGG	5 cut(s)	76, 147, 305, 876, 1676
SduI	GDGCHC	4 cut(s)	616, 858, 908, 1374
SfaNI	GCATC	3 cut(s)	49, 580, 787
SfiI	GGCCNNNNNGGCC	1 cut(s)	308
Sfr274I	CTCGAG	1 cut(s)	1596
SfuI	TTCGAA	1 cut(s)	140
SinI	GGWCC	5 cut(s)	20, 88, 149, 1138, 1528
SlaI	CTCGAG	1 cut(s)	1596
SmlI	CTYRAG	5 cut(s)	836, 1271, 1382, 1424, 1596
SmoI	CTYRAG	5 cut(s)	836, 1271, 1382, 1424, 1596
SphI	GCATGC	2 cut(s)	498, 759
SsiI	CCGC	3 cut(s)	266, 632, 1150
SspI	AATATT	1 cut(s)	1038
StyD4I	CCNGG	5 cut(s)	74, 145, 303, 874, 1674
StyI	CCWWGG	4 cut(s)	765, 1082, 1107, 1181
TaaI	ACNGT	1 cut(s)	1475
TaiI	ACGT	2 cut(s)	597, 1666
TaqI	TCGA	6 cut(s)	140, 675, 781, 1065, 1597, 1671
TatI	WGTACW	2 cut(s)	928, 1168
TfiI	GAWTC	3 cut(s)	142, 1223, 1430
Tru1I	TTAA	5 cut(s)	837, 1052, 1235, 1244, 1448
Tru9I	TTAA	5 cut(s)	837, 1052, 1235, 1244, 1448
TscAI	CASTG	3 cut(s)	867, 895, 1480
TseI	GCWGC	5 cut(s)	329, 716, 1505, 1553, 1606
TspDTI	ATGAA	9 cut(s)	31, 123, 353, 626, 1035, 1060, 1179, 1277, 1489
TspGWI	ACGGA	5 cut(s)	40, 141, 719, 949, 1040
TspRI	CASTG	3 cut(s)	867, 895, 1480
Van91I	CCANNNNNTGG	2 cut(s)	29, 766
Vha464I	CTTAAG	1 cut(s)	836
VpaK11BI	GGWCC	5 cut(s)	20, 88, 149, 1138, 1528
XagI	CCTNNNNNAGG	1 cut(s)	1523
XapI	RAATTY	2 cut(s)	136, 1706
XceI	RCATGY	4 cut(s)	15, 498, 759, 957
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1580, 1682
XhoI	CTCGAG	1 cut(s)	1596
XmiI	GTMKAC	2 cut(s)	1467, 1671
XmnI	GAANNNNTTC	1 cut(s)	1170
ZrmI	AGTACT	2 cut(s)	930, 1170
Zsp2I	ATGCAT	2 cut(s)	573, 780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.