MD12G1049200.v1.1

BTB POZ domain-containing protein At3g05675-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003442
Physical Location & Seq
Forward (+)
5626127 .. 5629123
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1049200.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGAGTATCTTGGTTCTGAAAAAAGTACTTCTAGCCAGATTGGGGACCGATCAACCAGTGATGTTGTTGTGAGGATACGCACAGAGGATGGGCGGGATGATCGGCTATATTGTCATTCCCAGATCCTCTTGGAAAAGAGCAAGTATTTCGCTGATCGCCTCTCTGAAAATTGGCCAACATGTCAGATTCTTGACTCACGCAACTGCGTTGACGTATACTGCCAAGAATCAGAATTTGACCACCATGTCAGTGTTCTCCGCCTTTTTTACGTCGCAGTAGATGGCTTAGTTGATAACTTGTGGCATGGTGTAAAAAACGCCATTGGAATTCTCCAGGTGGCTGTCAAGCTTGGATGCCCACAAATCATAACTGCTTGTGTGAACTACTTAGAAGCAGTTCCCTGGGAAGAGTCCGAGGAGGAGGAGATTTTAAAGATCGTACCACGCCTGGGATCACAAGTGGAGCCAATTCTTGCCCGTCTTCTTCCAGTCAATCACTCATCAATTATGGGGATATTTTTGTCAACCATCCGATTTGCCACATCGTCCCCTCCTCCAATCATGAATGATCTCAAGTCTTCTGCCCAGGAGCAGCTTGAGTACATGCTTACTGAAGATGATGATGCCCCGCTCTTAACAGCCGATGATGAAGTCAAATCCGAGGTGAAAGAATGTGTGAATAGACTATTTGGAAGATTTAGTAATCTATTAGAAGCTTTGTTATGTGAAGTAGAATTATCTTCTGATGGAGGGAAGATGCAGTCGTTTCAAACGCTTTTATCAGATCTGTCGTGGGCATGTCAGATATTAACTAAGTTGGAAACTATGAGGGAACTTGTCTGCAATTGGATGGATGCATCGGATAAAATAGTTAACGTTGTAAAGCAAGCAAGGCCGACAGCTGAACTAATTGAGACAAAGCTTAGGATTGTTGAGGTTGCAGCAAAGATTCTAGAGGCAATAGGTTACGGCACTGTCATTTTGCCAACTGCAAAACGGCTTCATATGGTTAAGGTGTGGCTTCCTTTTGTGAGGGTTGCGAGACCAGTGATCGATTCTGCCACAACTACCGGTGATGACGCTCCTACGCTAAAGATGGATAGTGAACTGTGGCAATCCTTGGAATCCACATTTGTTTCCATAATTCTTACACTTCCATCCGTCGAGCAGGCGGAGATATTGACTGAATGGTTGGAGAATGAACATATTCAATATCCTGACCTGACTGAGGCATTTGAGGTATGGTGTTACAGATCCAAGGTTTCCAAAAGAAGACTATCATTTATATCGGTGAACCATGAGAAAATCGATACACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

440

Amino Acids

49.7

Weight (kDa)

4.83

Isoelectric Point (pI)

51.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 220 - 420 6.1e-06 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AccBSI CCGCTC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 216
AciI CCGC 4 cut(s) 94, 259, 629, 1172
AclI AACGTT 1 cut(s) 876
AclWI GGATC 3 cut(s) 118, 460, 1248
AcoI YGGCCR 1 cut(s) 173
AcsI RAATTY 2 cut(s) 233, 327
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 3 cut(s) 28, 441, 602
AfiI CCNNNNNNNGG 3 cut(s) 43, 449, 1228
AflIII ACRYGT 1 cut(s) 179
AgeI ACCGGT 1 cut(s) 1070
AgsI TTSAA 2 cut(s) 770, 1211
AjnI CCWGG 4 cut(s) 333, 401, 447, 585
AluBI AGCT 5 cut(s) 349, 595, 716, 902, 922
AluI AGCT 5 cut(s) 349, 595, 716, 902, 922
Alw26I GTCTC 2 cut(s) 908, 1036
AlwI GGATC 3 cut(s) 118, 460, 1248
AoxI GGCC 2 cut(s) 173, 893
ApeKI GCWGC 2 cut(s) 592, 941
ApoI RAATTY 2 cut(s) 233, 327
AsiGI ACCGGT 1 cut(s) 1070
Asp700I GAANNNNTTC 2 cut(s) 396, 1206
AspS9I GGNCC 1 cut(s) 46
AsuHPI GGTGA 3 cut(s) 676, 1085, 1303
AvaII GGWCC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 175
BbsI GAAGAC 3 cut(s) 473, 570, 1279
BbvI GCAGC 2 cut(s) 604, 953
BccI CCATC 7 cut(s) 83, 275, 536, 740, 844, 1090, 1165
BceAI ACGGC 2 cut(s) 985, 1013
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 4 cut(s) 335, 403, 449, 587
BciVI GTATCC 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 908, 1036
BfaI CTAG 2 cut(s) 33, 953
BfuI GTATCC 1 cut(s) 69
BglII AGATCT 1 cut(s) 784
BisI GCNGC 2 cut(s) 593, 942
BlsI GCNGC 2 cut(s) 594, 943
BmcAI AGTACT 1 cut(s) 28
Bme1390I CCNGG 4 cut(s) 335, 403, 449, 587
Bme18I GGWCC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 46
BmiI GGNNCC 2 cut(s) 47, 465
BmrFI CCNGG 4 cut(s) 335, 403, 449, 587
BmsI GCATC 5 cut(s) 344, 613, 747, 844, 866
BpiI GAAGAC 3 cut(s) 473, 570, 1279
BpmI CTGGAG 1 cut(s) 317
Bpu10I CCTNAGC 1 cut(s) 923
BpuEI CTTGAG 2 cut(s) 557, 617
Bsa29I ATCGAT 2 cut(s) 1053, 1308
BsaI GGTCTC 1 cut(s) 1036
BsaJI CCNNGG 8 cut(s) 401, 402, 414, 448, 585, 660, 1119, 1257
BsaWI WCCGGW 1 cut(s) 1070
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 449, 1228
Bse118I RCCGGY 1 cut(s) 1070
Bse1I ACTGG 3 cut(s) 57, 488, 1046
BseBI CCWGG 4 cut(s) 335, 403, 449, 587
BseCI ATCGAT 2 cut(s) 1053, 1308
BseDI CCNNGG 8 cut(s) 401, 402, 414, 448, 585, 660, 1119, 1257
BseGI GGATG 7 cut(s) 94, 103, 359, 528, 855, 859, 1157
BseLI CCNNNNNNNGG 3 cut(s) 43, 449, 1228
BseMII CTCAG 1 cut(s) 1218
BseNI ACTGG 3 cut(s) 57, 488, 1046
BseRI GAGGAG 4 cut(s) 431, 434, 437, 543
BseXI GCAGC 2 cut(s) 604, 953
BshFI GGCC 2 cut(s) 175, 895
BshTI ACCGGT 1 cut(s) 1070
BshVI ATCGAT 2 cut(s) 1053, 1308
BsiSI CCGG 1 cut(s) 1071
BslFI GGGAC 2 cut(s) 59, 532
BslI CCNNNNNNNGG 3 cut(s) 43, 449, 1228
BsmAI GTCTC 2 cut(s) 908, 1036
BsmFI GGGAC 2 cut(s) 59, 532
BsnI GGCC 2 cut(s) 175, 895
Bso31I GGTCTC 1 cut(s) 1036
BspACI CCGC 4 cut(s) 94, 259, 629, 1172
BspANI GGCC 2 cut(s) 175, 895
BspCNI CTCAG 1 cut(s) 1219
BspDI ATCGAT 2 cut(s) 1053, 1308
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 2 cut(s) 47, 465
BspPI GGATC 3 cut(s) 118, 460, 1248
BspTNI GGTCTC 1 cut(s) 1036
BsrBI CCGCTC 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 1070
BsrI ACTGG 3 cut(s) 57, 488, 1046
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 8 cut(s) 401, 402, 414, 448, 585, 660, 1119, 1257
BssNAI GTATAC 1 cut(s) 217
BssT1I CCWWGG 2 cut(s) 1119, 1257
Bst1107I GTATAC 1 cut(s) 217
Bst2UI CCWGG 4 cut(s) 335, 403, 449, 587
Bst4CI ACNGT 2 cut(s) 976, 1110
Bst6I CTCTTC 1 cut(s) 402
BstC8I GCNNGC 2 cut(s) 888, 1170
BstDEI CTNAG 5 cut(s) 286, 388, 813, 923, 1227
BstENI CCTNNNNNAGG 1 cut(s) 1226
BstF5I GGATG 7 cut(s) 94, 103, 359, 528, 855, 859, 1157
BstMAI GTCTC 2 cut(s) 908, 1036
BstMWI GCNNNNNNNGC 1 cut(s) 892
BstNI CCWGG 4 cut(s) 335, 403, 449, 587
BstNSI RCATGY 3 cut(s) 183, 607, 801
BstSCI CCNGG 4 cut(s) 333, 401, 447, 585
BstV1I GCAGC 2 cut(s) 604, 953
BstV2I GAAGAC 3 cut(s) 473, 570, 1279
BstX2I RGATCY 3 cut(s) 123, 784, 1253
BstYI RGATCY 3 cut(s) 123, 784, 1253
BstZ17I GTATAC 1 cut(s) 217
Bsu15I ATCGAT 2 cut(s) 1053, 1308
BsuI GTATCC 1 cut(s) 69
BsuRI GGCC 2 cut(s) 175, 895
BsuTUI ATCGAT 2 cut(s) 1053, 1308
BtsCI GGATG 7 cut(s) 94, 103, 359, 528, 855, 859, 1157
BtsIMutI CAGTG 4 cut(s) 64, 256, 972, 1053
Cac8I GCNNGC 2 cut(s) 888, 1170
CciI TCATGA 1 cut(s) 561
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 1 cut(s) 46
ClaI ATCGAT 2 cut(s) 1053, 1308
CseI GACGC 1 cut(s) 1088
Csp6I GTAC 3 cut(s) 27, 440, 601
CspAI ACCGGT 1 cut(s) 1070
CviAII CATG 7 cut(s) 180, 245, 305, 562, 604, 798, 1298
CviQI GTAC 3 cut(s) 27, 440, 601
DdeI CTNAG 5 cut(s) 286, 388, 813, 923, 1227
DraI TTTAAA 1 cut(s) 432
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 173
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 2 cut(s) 248, 1187
Eco130I CCWWGG 2 cut(s) 1119, 1257
Eco31I GGTCTC 1 cut(s) 1036
Eco47I GGWCC 1 cut(s) 46
Eco57I CTGAAG 1 cut(s) 633
EcoNI CCTNNNNNAGG 1 cut(s) 1226
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 4 cut(s) 333, 401, 447, 585
EcoT14I CCWWGG 2 cut(s) 1119, 1257
EcoT22I ATGCAT 1 cut(s) 859
ErhI CCWWGG 2 cut(s) 1119, 1257
FaeI CATG 7 cut(s) 183, 248, 308, 565, 607, 801, 1301
FaqI GGGAC 2 cut(s) 59, 532
FatI CATG 7 cut(s) 179, 244, 304, 561, 603, 797, 1297
FauI CCCGC 2 cut(s) 87, 636
FauNDI CATATG 1 cut(s) 1005
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 593, 942
FokI GGATG 7 cut(s) 101, 110, 366, 515, 862, 866, 1144
Fsp4HI GCNGC 2 cut(s) 593, 942
FspBI CTAG 2 cut(s) 33, 953
GluI GCNGC 2 cut(s) 593, 942
GsuI CTGGAG 1 cut(s) 317
HaeIII GGCC 2 cut(s) 175, 895
HapII CCGG 1 cut(s) 1071
HgaI GACGC 1 cut(s) 1088
Hin1II CATG 7 cut(s) 183, 248, 308, 565, 607, 801, 1301
HincII GTYRAC 3 cut(s) 211, 525, 874
HindII GTYRAC 3 cut(s) 211, 525, 874
HindIII AAGCTT 3 cut(s) 347, 714, 920
HinfI GANTC 7 cut(s) 187, 194, 227, 410, 949, 1055, 1124
HpaI GTTAAC 1 cut(s) 874
HpaII CCGG 1 cut(s) 1071
HphI GGTGA 3 cut(s) 676, 1085, 1303
Hpy166II GTNNAC 7 cut(s) 211, 217, 382, 525, 874, 1106, 1294
Hpy188III TCNNGA 4 cut(s) 191, 562, 953, 1217
Hpy8I GTNNAC 7 cut(s) 211, 217, 382, 525, 874, 1106, 1294
Hpy99I CGWCG 2 cut(s) 275, 1166
HpyCH4III ACNGT 2 cut(s) 976, 1110
HpyCH4IV ACGT 3 cut(s) 213, 270, 876
HpyCH4V TGCA 5 cut(s) 760, 843, 857, 941, 992
HpyF10VI GCNNNNNNNGC 1 cut(s) 892
HpyF3I CTNAG 5 cut(s) 286, 388, 813, 923, 1227
HpySE526I ACGT 3 cut(s) 213, 270, 876
Hsp92II CATG 7 cut(s) 183, 248, 308, 565, 607, 801, 1301
KspAI GTTAAC 1 cut(s) 874
LmnI GCTCC 3 cut(s) 463, 589, 1087
Lsp1109I GCAGC 2 cut(s) 604, 953
LweI GCATC 5 cut(s) 344, 613, 747, 844, 866
MaeI CTAG 2 cut(s) 33, 953
MaeII ACGT 3 cut(s) 213, 270, 876
MaeIII GTNAC 2 cut(s) 965, 1247
MbiI CCGCTC 1 cut(s) 631
MboII GAAGA 9 cut(s) 419, 473, 476, 570, 626, 705, 732, 766, 1284
MfeI CAATTG 1 cut(s) 844
MflI RGATCY 3 cut(s) 123, 784, 1253
MlsI TGGCCA 1 cut(s) 175
MluCI AATT 9 cut(s) 169, 233, 327, 468, 504, 734, 844, 909, 1143
MluNI TGGCCA 1 cut(s) 175
MlyI GAGTC 2 cut(s) 188, 419
MmeI TCCRAC 2 cut(s) 798, 1173
Mox20I TGGCCA 1 cut(s) 175
Mph1103I ATGCAT 1 cut(s) 859
MroXI GAANNNNTTC 2 cut(s) 396, 1206
MscI TGGCCA 1 cut(s) 175
MseI TTAA 6 cut(s) 431, 635, 809, 873, 1011, 1318
MslI CAYNNNNRTG 1 cut(s) 249
Msp20I TGGCCA 1 cut(s) 175
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 1 cut(s) 1071
MspR9I CCNGG 4 cut(s) 335, 403, 449, 587
MunI CAATTG 1 cut(s) 844
MvaI CCWGG 4 cut(s) 335, 403, 449, 587
MwoI GCNNNNNNNGC 1 cut(s) 892
NdeI CATATG 1 cut(s) 1005
NlaIII CATG 7 cut(s) 183, 248, 308, 565, 607, 801, 1301
NlaIV GGNNCC 2 cut(s) 47, 465
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 3 cut(s) 183, 607, 801
PagI TCATGA 1 cut(s) 561
PasI CCCWGGG 1 cut(s) 402
PciI ACATGT 1 cut(s) 179
PdmI GAANNNNTTC 2 cut(s) 396, 1206
PfeI GAWTC 5 cut(s) 187, 227, 949, 1055, 1124
PinAI ACCGGT 1 cut(s) 1070
PkrI GCNGC 2 cut(s) 594, 943
PleI GAGTC 2 cut(s) 188, 418
PpsI GAGTC 2 cut(s) 188, 418
PscI ACATGT 1 cut(s) 179
Psp1406I AACGTT 1 cut(s) 876
Psp6I CCWGG 4 cut(s) 333, 401, 447, 585
PspGI CCWGG 4 cut(s) 333, 401, 447, 585
PspN4I GGNNCC 2 cut(s) 47, 465
PspPI GGNCC 1 cut(s) 46
PsuI RGATCY 3 cut(s) 123, 784, 1253
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 3 cut(s) 28, 441, 602
RsaNI GTAC 3 cut(s) 27, 440, 601
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 6 cut(s) 431, 635, 809, 873, 1011, 1318
SatI GCNGC 2 cut(s) 593, 942
Sau96I GGNCC 1 cut(s) 46
ScaI AGTACT 1 cut(s) 28
SchI GAGTC 2 cut(s) 188, 419
ScrFI CCNGG 4 cut(s) 335, 403, 449, 587
SfaNI GCATC 5 cut(s) 344, 613, 747, 844, 866
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 572, 596
SmoI CTYRAG 2 cut(s) 572, 596
Sse9I AATT 9 cut(s) 169, 233, 327, 468, 504, 734, 844, 909, 1143
SsiI CCGC 4 cut(s) 94, 259, 629, 1172
SspMI CTAG 2 cut(s) 33, 953
StyD4I CCNGG 4 cut(s) 333, 401, 447, 585
StyI CCWWGG 2 cut(s) 1119, 1257
TaaI ACNGT 2 cut(s) 976, 1110
TaiI ACGT 3 cut(s) 216, 273, 879
TaqI TCGA 3 cut(s) 1053, 1164, 1308
TaqII GACCGA 1 cut(s) 63
TasI AATT 9 cut(s) 169, 233, 327, 468, 504, 734, 844, 909, 1143
TatI WGTACW 2 cut(s) 26, 600
TfiI GAWTC 5 cut(s) 187, 227, 949, 1055, 1124
Tru1I TTAA 6 cut(s) 431, 635, 809, 873, 1011, 1318
Tru9I TTAA 6 cut(s) 431, 635, 809, 873, 1011, 1318
TscAI CASTG 4 cut(s) 64, 256, 979, 1053
TseI GCWGC 2 cut(s) 592, 941
TspDTI ATGAA 4 cut(s) 578, 663, 992, 1215
TspGWI ACGGA 1 cut(s) 1150
TspRI CASTG 4 cut(s) 64, 256, 979, 1053
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 1226
XapI RAATTY 2 cut(s) 233, 327
XbaI TCTAGA 1 cut(s) 952
XceI RCATGY 3 cut(s) 183, 607, 801
XmiI GTMKAC 1 cut(s) 216
XmnI GAANNNNTTC 2 cut(s) 396, 1206
XspI CTAG 2 cut(s) 33, 953
ZrmI AGTACT 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.