pycom14g03960

BTB POZ domain-containing protein At3g05675-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003442
Physical Location & Seq
Forward (+)
3433911 .. 3435101
1191 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g03960.1

Sequence Viewer

Length: 1191 bp
ATGGAAAAGAGCAAGTATTTCGCCGATCGCCTCTCTGAAAATTGGCCAACATGTCAGATTCTTGACTCACGCAACTGCGTTGACGTATACTGCCAAGAATCAGAATTTGACCACCATGTCAGTGTTCTCCGCCTTTTTTACATCATAGTAGATGGCTTACTTGATGACTTGTGGCATGGTGTAAAAAACGCCATTGGAATTCTCCAGGTGGCTGTCAAGCTTGGATGCCCACAAATTGTAACTGCTTGTGTGAACTACTTGGAAGCAGTTCCCTGGGAAGAGTCTGAGGAGGAGGAGATTTTAAAGATCATACCACGGCTGGGATCACAAGTGGAGCCAATTCTTGCCCGTCTTCTTCCAGTCAATCACTCATCAATCATGGCGATATTTTTGTCAACCATCCGATTTGCCACATCGTCCCCTCTTCCAATCATGAATGATCTCAAGTCTTCTGCCCAGGAGCAGCTTGAGTACATGCTGACTGAAGACGATGATGCCCCTCTCCTAACAGCCGATGATGAGATTAAATCCGAGGTGAAAGAATGTGTGAAGAGACTATATGGAAGATTTAAGAATCTATTAGAAGCTTTGTTAAGTGAAGTAGAATTATCTTCTGATGGAGGGAAGATGCAATCATTTCAATCGTTTTTATCAGATCTGTTGTGGGCGTGCCAGATTTTAACTAAGTTGGAAATTATGAGGGAACTTGTCTGCAATTGGATGGATGCATCGGATAAAATAGTTAAGGTTGTAAAGCAAGCAAGGCCGACAACTGAACTGATTGAGACAAAGCTTAGGGTTGTTGAGGTTGCAGCAAAGATTCTAGAGGCAATAGGTTACGGCACTGTGATTTTGCCAACTGCAAAACGGCTTCATATGGTAAAGGTGTGGCTTCCTTTTGTGAGGGTTGCGAAACCACTGATCGATTCTGCCACAACTACCGGTGATGATGCTCCTACGCTAAAGATGGATAGTGAACTGTGGCAATCCTTGGAATCCACTTTTGTTTCCATAATACTTGCACTCCCATCTGTTGAGCAGGCGGAGATATTGACCGAATGGTTGGAGAACGAACATATTCAATATCCTGACCTGACTGAGGCATTTGAGGTATGGTGCTACAGATCCAAGGTTTCCAAAAGAAGACTATCGTTTATATCGGTGAACCATGACAAAATCGATACACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

397

Amino Acids

45.03

Weight (kDa)

4.89

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 174 - 377 6.8e-06 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 87
AciI CCGC 2 cut(s) 130, 1043
AclWI GGATC 2 cut(s) 331, 1119
AcoI YGGCCR 1 cut(s) 44
AcsI RAATTY 2 cut(s) 104, 198
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 2 cut(s) 320, 1099
AflIII ACRYGT 1 cut(s) 50
AgeI ACCGGT 1 cut(s) 941
AgsI TTSAA 2 cut(s) 641, 1082
AjnI CCWGG 3 cut(s) 204, 272, 456
AluBI AGCT 4 cut(s) 220, 466, 587, 793
AluI AGCT 4 cut(s) 220, 466, 587, 793
Alw26I GTCTC 2 cut(s) 547, 779
AlwI GGATC 2 cut(s) 331, 1119
AoxI GGCC 2 cut(s) 44, 764
ApeKI GCWGC 2 cut(s) 463, 812
ApoI RAATTY 2 cut(s) 104, 198
AsiGI ACCGGT 1 cut(s) 941
Asp700I GAANNNNTTC 2 cut(s) 267, 1077
AsuHPI GGTGA 3 cut(s) 547, 956, 1174
BalI TGGCCA 1 cut(s) 46
BbsI GAAGAC 4 cut(s) 344, 441, 492, 1150
BbvI GCAGC 2 cut(s) 475, 824
BccI CCATC 6 cut(s) 146, 407, 611, 715, 961, 1036
BceAI ACGGC 3 cut(s) 332, 856, 884
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 3 cut(s) 206, 274, 458
BcoDI GTCTC 2 cut(s) 547, 779
BfaI CTAG 1 cut(s) 824
BfmI CTRYAG 1 cut(s) 1120
BglII AGATCT 1 cut(s) 655
BisI GCNGC 2 cut(s) 464, 813
BlsI GCNGC 2 cut(s) 465, 814
Bme1390I CCNGG 3 cut(s) 206, 274, 458
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 3 cut(s) 206, 274, 458
BmsI GCATC 6 cut(s) 215, 484, 618, 715, 737, 940
BpiI GAAGAC 4 cut(s) 344, 441, 492, 1150
BpmI CTGGAG 1 cut(s) 188
Bpu10I CCTNAGC 1 cut(s) 794
BpuEI CTTGAG 2 cut(s) 428, 488
Bsa29I ATCGAT 2 cut(s) 924, 1179
BsaJI CCNNGG 7 cut(s) 272, 273, 314, 456, 531, 990, 1128
BsaWI WCCGGW 1 cut(s) 941
BsaXI ACNNNNNCTCC 2 cut(s) 1008, 1038
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 1099
Bse118I RCCGGY 1 cut(s) 941
Bse1I ACTGG 1 cut(s) 359
BseBI CCWGG 3 cut(s) 206, 274, 458
BseCI ATCGAT 2 cut(s) 924, 1179
BseDI CCNNGG 7 cut(s) 272, 273, 314, 456, 531, 990, 1128
BseGI GGATG 4 cut(s) 230, 399, 726, 730
BseLI CCNNNNNNNGG 2 cut(s) 320, 1099
BseMII CTCAG 2 cut(s) 276, 1089
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 3 cut(s) 302, 305, 308
BseXI GCAGC 2 cut(s) 475, 824
BseYI CCCAGC 1 cut(s) 319
Bsh1285I CGRYCG 1 cut(s) 28
BshFI GGCC 2 cut(s) 46, 766
BshTI ACCGGT 1 cut(s) 941
BshVI ATCGAT 2 cut(s) 924, 1179
BsiEI CGRYCG 1 cut(s) 28
BsiSI CCGG 1 cut(s) 942
BslFI GGGAC 1 cut(s) 403
BslI CCNNNNNNNGG 2 cut(s) 320, 1099
BsmAI GTCTC 2 cut(s) 547, 779
BsmFI GGGAC 1 cut(s) 403
BsnI GGCC 2 cut(s) 46, 766
Bsp143I GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
BspACI CCGC 2 cut(s) 130, 1043
BspANI GGCC 2 cut(s) 46, 766
BspCNI CTCAG 2 cut(s) 277, 1090
BspDI ATCGAT 2 cut(s) 924, 1179
BspHI TCATGA 1 cut(s) 432
BspLI GGNNCC 1 cut(s) 336
BspPI GGATC 2 cut(s) 331, 1119
BsrFI RCCGGY 1 cut(s) 941
BsrI ACTGG 1 cut(s) 359
BssAI RCCGGY 1 cut(s) 941
BssECI CCNNGG 7 cut(s) 272, 273, 314, 456, 531, 990, 1128
BssMI GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
BssNAI GTATAC 1 cut(s) 88
BssT1I CCWWGG 2 cut(s) 990, 1128
Bst1107I GTATAC 1 cut(s) 88
Bst2UI CCWGG 3 cut(s) 206, 274, 458
Bst4CI ACNGT 2 cut(s) 847, 981
Bst6I CTCTTC 3 cut(s) 273, 429, 545
BstC8I GCNNGC 3 cut(s) 670, 759, 1041
BstDEI CTNAG 4 cut(s) 285, 684, 794, 1098
BstDSI CCRYGG 1 cut(s) 314
BstENI CCTNNNNNAGG 1 cut(s) 1097
BstF5I GGATG 4 cut(s) 230, 399, 726, 730
BstKTI GATC 7 cut(s) 28, 309, 326, 442, 658, 924, 1127
BstMAI GTCTC 2 cut(s) 547, 779
BstMBI GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
BstMCI CGRYCG 1 cut(s) 28
BstMWI GCNNNNNNNGC 1 cut(s) 763
BstNI CCWGG 3 cut(s) 206, 274, 458
BstNSI RCATGY 2 cut(s) 54, 478
BstSCI CCNGG 3 cut(s) 204, 272, 456
BstSFI CTRYAG 1 cut(s) 1120
BstV1I GCAGC 2 cut(s) 475, 824
BstV2I GAAGAC 4 cut(s) 344, 441, 492, 1150
BstX2I RGATCY 2 cut(s) 655, 1124
BstYI RGATCY 2 cut(s) 655, 1124
BstZ17I GTATAC 1 cut(s) 88
Bsu15I ATCGAT 2 cut(s) 924, 1179
BsuRI GGCC 2 cut(s) 46, 766
BsuTUI ATCGAT 2 cut(s) 924, 1179
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 4 cut(s) 230, 399, 726, 730
BtsIMutI CAGTG 3 cut(s) 127, 843, 917
Cac8I GCNNGC 3 cut(s) 670, 759, 1041
CciI TCATGA 1 cut(s) 432
Cfr10I RCCGGY 1 cut(s) 941
ClaI ATCGAT 2 cut(s) 924, 1179
Csp6I GTAC 1 cut(s) 472
CspAI ACCGGT 1 cut(s) 941
CviAII CATG 7 cut(s) 51, 116, 176, 379, 433, 475, 1169
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 4 cut(s) 285, 684, 794, 1098
DpnI GATC 7 cut(s) 27, 308, 325, 441, 657, 923, 1126
DpnII GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
DraI TTTAAA 1 cut(s) 303
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 44
Eam1104I CTCTTC 3 cut(s) 273, 429, 545
EarI CTCTTC 3 cut(s) 273, 429, 545
EciI GGCGGA 2 cut(s) 119, 1058
Eco130I CCWWGG 2 cut(s) 990, 1128
Eco57I CTGAAG 1 cut(s) 504
EcoNI CCTNNNNNAGG 1 cut(s) 1097
EcoRI GAATTC 1 cut(s) 198
EcoRII CCWGG 3 cut(s) 204, 272, 456
EcoT14I CCWWGG 2 cut(s) 990, 1128
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 990, 1128
FaeI CATG 7 cut(s) 54, 119, 179, 382, 436, 478, 1172
FaqI GGGAC 1 cut(s) 403
FatI CATG 7 cut(s) 50, 115, 175, 378, 432, 474, 1168
FauNDI CATATG 1 cut(s) 876
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 464, 813
FokI GGATG 4 cut(s) 237, 386, 733, 737
Fsp4HI GCNGC 2 cut(s) 464, 813
FspBI CTAG 1 cut(s) 824
GluI GCNGC 2 cut(s) 464, 813
GsaI CCCAGC 1 cut(s) 323
GsuI CTGGAG 1 cut(s) 188
HaeIII GGCC 2 cut(s) 46, 766
HapII CCGG 1 cut(s) 942
Hin1II CATG 7 cut(s) 54, 119, 179, 382, 436, 478, 1172
HincII GTYRAC 2 cut(s) 82, 396
HindII GTYRAC 2 cut(s) 82, 396
HindIII AAGCTT 3 cut(s) 218, 585, 791
HinfI GANTC 8 cut(s) 58, 65, 98, 281, 574, 820, 926, 995
HpaII CCGG 1 cut(s) 942
HphI GGTGA 3 cut(s) 547, 956, 1174
Hpy166II GTNNAC 6 cut(s) 82, 88, 253, 396, 977, 1165
Hpy188I TCNGA 9 cut(s) 37, 57, 103, 286, 404, 532, 616, 655, 733
Hpy188III TCNNGA 4 cut(s) 62, 433, 824, 1088
Hpy8I GTNNAC 6 cut(s) 82, 88, 253, 396, 977, 1165
HpyCH4III ACNGT 2 cut(s) 847, 981
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 6 cut(s) 631, 714, 728, 812, 863, 1022
HpyF10VI GCNNNNNNNGC 1 cut(s) 763
HpyF3I CTNAG 4 cut(s) 285, 684, 794, 1098
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 7 cut(s) 54, 119, 179, 382, 436, 478, 1172
Kzo9I GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
LmnI GCTCC 3 cut(s) 334, 460, 958
Lsp1109I GCAGC 2 cut(s) 475, 824
LweI GCATC 6 cut(s) 215, 484, 618, 715, 737, 940
MaeI CTAG 1 cut(s) 824
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 2 cut(s) 238, 836
MalI GATC 7 cut(s) 27, 308, 325, 441, 657, 923, 1126
MboI GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
MfeI CAATTG 1 cut(s) 715
MflI RGATCY 2 cut(s) 655, 1124
MlsI TGGCCA 1 cut(s) 46
MluCI AATT 8 cut(s) 40, 104, 198, 234, 339, 605, 693, 715
MluNI TGGCCA 1 cut(s) 46
MlyI GAGTC 2 cut(s) 59, 290
MmeI TCCRAC 2 cut(s) 669, 1044
Mox20I TGGCCA 1 cut(s) 46
Mph1103I ATGCAT 1 cut(s) 730
MroXI GAANNNNTTC 2 cut(s) 267, 1077
MscI TGGCCA 1 cut(s) 46
MseI TTAA 7 cut(s) 302, 525, 570, 593, 680, 744, 1189
MslI CAYNNNNRTG 1 cut(s) 120
Msp20I TGGCCA 1 cut(s) 46
MspI CCGG 1 cut(s) 942
MspR9I CCNGG 3 cut(s) 206, 274, 458
MunI CAATTG 1 cut(s) 715
MvaI CCWGG 3 cut(s) 206, 274, 458
MwoI GCNNNNNNNGC 1 cut(s) 763
NdeI CATATG 1 cut(s) 876
NdeII GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
NlaIII CATG 7 cut(s) 54, 119, 179, 382, 436, 478, 1172
NlaIV GGNNCC 1 cut(s) 336
NsiI ATGCAT 1 cut(s) 730
NspI RCATGY 2 cut(s) 54, 478
PagI TCATGA 1 cut(s) 432
PasI CCCWGGG 1 cut(s) 273
PciI ACATGT 1 cut(s) 50
PdmI GAANNNNTTC 2 cut(s) 267, 1077
PfeI GAWTC 6 cut(s) 58, 98, 574, 820, 926, 995
PinAI ACCGGT 1 cut(s) 941
PkrI GCNGC 2 cut(s) 465, 814
Ple19I CGATCG 1 cut(s) 28
PleI GAGTC 2 cut(s) 59, 289
PpsI GAGTC 2 cut(s) 59, 289
PscI ACATGT 1 cut(s) 50
Psp6I CCWGG 3 cut(s) 204, 272, 456
PspFI CCCAGC 1 cut(s) 319
PspGI CCWGG 3 cut(s) 204, 272, 456
PspN4I GGNNCC 1 cut(s) 336
PsuI RGATCY 2 cut(s) 655, 1124
PvuI CGATCG 1 cut(s) 28
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 1 cut(s) 120
SaqAI TTAA 7 cut(s) 302, 525, 570, 593, 680, 744, 1189
SatI GCNGC 2 cut(s) 464, 813
Sau3AI GATC 7 cut(s) 25, 306, 323, 439, 655, 921, 1124
SchI GAGTC 2 cut(s) 59, 290
ScrFI CCNGG 3 cut(s) 206, 274, 458
SfaNI GCATC 6 cut(s) 215, 484, 618, 715, 737, 940
SfcI CTRYAG 1 cut(s) 1120
SmiMI CAYNNNNRTG 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 443, 467
SmoI CTYRAG 2 cut(s) 443, 467
Sse9I AATT 8 cut(s) 40, 104, 198, 234, 339, 605, 693, 715
SsiI CCGC 2 cut(s) 130, 1043
SspMI CTAG 1 cut(s) 824
StyD4I CCNGG 3 cut(s) 204, 272, 456
StyI CCWWGG 2 cut(s) 990, 1128
TaaI ACNGT 2 cut(s) 847, 981
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 924, 1179
TaqII GACCGA 1 cut(s) 1070
TasI AATT 8 cut(s) 40, 104, 198, 234, 339, 605, 693, 715
TatI WGTACW 1 cut(s) 471
TfiI GAWTC 6 cut(s) 58, 98, 574, 820, 926, 995
Tru1I TTAA 7 cut(s) 302, 525, 570, 593, 680, 744, 1189
Tru9I TTAA 7 cut(s) 302, 525, 570, 593, 680, 744, 1189
TscAI CASTG 3 cut(s) 127, 850, 924
TseI GCWGC 2 cut(s) 463, 812
TspDTI ATGAA 2 cut(s) 449, 863
TspRI CASTG 3 cut(s) 127, 850, 924
XagI CCTNNNNNAGG 1 cut(s) 1097
XapI RAATTY 2 cut(s) 104, 198
XbaI TCTAGA 1 cut(s) 823
XceI RCATGY 2 cut(s) 54, 478
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 2 cut(s) 267, 1077
XspI CTAG 1 cut(s) 824
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.