Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009156

Physical Location & Seq

Genomic Coordinates Reverse (-)

6535170 .. 6544352

Gene Structure 9183 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1057200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGAAGCCATGGAAATCGATTCGGAGAAGAACCTCCACAGAAAGCATTCCGCCTACACCTTGCTGGACGAGAGGTTTGAGATTCAGAAGGAAATGTACAGGGGTCAACAGTACAGCCAAATATACTTCGTCCGTCTACATATGATGAGGACGCTCCTCTATTCCCTTGTCCCTAACTGGAAACCCCATTTGCCAGTTCGCACAGTTTTGGGATTGGAAAGGGACAAGGAATGCATCATTGTTGGAACCCTCTACAAGCACATGAAACTCAAGCCTTGTGTTCTTGATGAGTACTCTAAGACGAGATCTGTGACACCACTTGTCAAGCCTCATAATTTTGTGCACCCAGATGACAATCTGATTTTGGAAGATGAAAGTGGAAGAGTTAATCTTTCCGGGAATGTGCTTTCACCTACTGTGTATGTAACAGGGACTGTTGTTGGTTTACATGGAAAGGAGACTGATGCCGGTGACTTCTTAGTCCTAGATGTCCTTGAAGCTGGCTTACCACCCCAGATAGAATTGCCACTGAAATCAAGAGAAGGCAAATATGTTGTTTTTGTATCTGGACTTCGTGTTGGAAGCAGCTCTTTAAACCCTCTGCAGTTTCAGCTTCTTGTTGATCATATAACTGGACATTTAGGAGATGAGAAGGAACAAGGTATTGCAGCACAAATAGTTCATGTTGTAATTGCTGGAAATTCTATTGAGGTTCCTCATGGACTTCTTAATGGACAGAATTTAGCGTTAAAGGACCAATCAAGGTTGTCTGAGCCAATTAAGGAGTTGGATATCTTGTTGACTCAGATTGCTGCAGGTTTGCCTTTGGATATCATGCCTGGTCTCGATGACCCTGCTAATTTTGCCTTGCCACAGCAGCCTTTGAACAGATGCCTTTTCCCTGGGTCATCGGCTTATAACACGTTTAGATCATGTACCAATCCTCATTGCTTTGATCTTGACGATGTCAGGTTTCTTGGAACATCGGGGCAAAATATAGATGATCTTGATAAGTATTCAGAAGCGAAGGATAAACTTGATTTTATTGAAAGGACATTGAGGTGGAGGCATCTGGCACCAACAGCACCTAATACCCTTGGGTGTTATCCTTTCACGGACAGGGATCCATTCCTGATCGAGAGTTGTCCTCACGTTTACTTTGTTGGTAATCAGGATAAATATGATAGTCGCTTAGTAAATGGATCTGAAGGGCAGATGGTGAGACTCATTAGCATTCCTAAATTCTGTGAGACGGGAATTGCTGTTGTGTTAAACTTGGGGAATCTTGAATGCCATACACTCAGTTTTGGGACTCAATTCAGCTCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0022616 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_E_B

Protein Analysis

443

Amino Acids

49.54

Weight (kDa)

5.66

Isoelectric Point (pI)

37.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_D_N	PF18018	37 - 164	3.9e-39	DNA polymerase delta subunit OB-fold domain
DNA_pol_E_B	PF04042	185 - 394	1.2e-51	DNA polymerase alpha/epsilon subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42120 AT2G42120
fragaria_vesca	FvH4_6g22330 FvH4_6g22330
malus_domestica	MD12G1057200.v1.1 MD14G1057800.v1.1
prunus_persica	Prupe.7G076900_v2.0.a1 Prupe.7G076900_v2.0.a1 Prupe.7G076900_v2.0.a1
pyrus_communis	pycom14g04690
rosa_chinensis	RchiOBHm_Chr3g0479691
rosa_multiflora	Rmu_sc0003755.1_g000006 Rmu_sc0031395.1_g000006
rosa_roxburghii	Rroxscaffold_6G00402060
rosa_rugosa	Rorug03G0177400 Rorug03G0177500 Rorug03G0177600
rosa_samantha	Rh3AG228200 Rh3BG261400 Rh3CG258500 Rh3DG254700
rosa_wichuraiana	Rw3G020420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	918
AasI	GACNNNNNNGTC	1 cut(s)	479
Acc36I	ACCTGC	1 cut(s)	806
AccB1I	GGYRCC	1 cut(s)	1075
AccI	GTMKAC	1 cut(s)	136
AciI	CCGC	1 cut(s)	51
AclWI	GGATC	3 cut(s)	1118, 1131, 1210
AcsI	RAATTY	3 cut(s)	700, 739, 1241
AcuI	CTGAAG	1 cut(s)	1227
AfaI	GTAC	4 cut(s)	98, 113, 293, 937
AflIII	ACRYGT	1 cut(s)	921
AgsI	TTSAA	4 cut(s)	497, 886, 1049, 1289
AjnI	CCWGG	2 cut(s)	838, 901
AluBI	AGCT	4 cut(s)	500, 588, 613, 1323
AluI	AGCT	4 cut(s)	500, 588, 613, 1323
Alw21I	GWGCWC	1 cut(s)	345
Alw26I	GTCTC	4 cut(s)	452, 848, 1216, 1244
Alw44I	GTGCAC	1 cut(s)	341
AlwI	GGATC	3 cut(s)	1118, 1131, 1210
AlwNI	CAGNNNCTG	1 cut(s)	434
ApaLI	GTGCAC	1 cut(s)	341
ApeKI	GCWGC	4 cut(s)	585, 668, 812, 877
ApoI	RAATTY	3 cut(s)	700, 739, 1241
ArsI	GACNNNNNNTTYG	2 cut(s)	59, 91
Asp700I	GAANNNNTTC	1 cut(s)	46
AspS9I	GGNCC	1 cut(s)	754
AsuC2I	CCSGG	1 cut(s)	397
AsuHPI	GGTGA	3 cut(s)	402, 482, 1231
AvaII	GGWCC	1 cut(s)	754
BaeGI	GKGCMC	1 cut(s)	345
BamHI	GGATCC	1 cut(s)	1123
BanI	GGYRCC	1 cut(s)	1075
BarI	GAAGNNNNNNTAC	6 cut(s)	80, 112, 489, 521, 555, 587
Bbv12I	GWGCWC	1 cut(s)	345
BbvI	GCAGC	4 cut(s)	597, 680, 799, 889
BccI	CCATC	1 cut(s)	1210
BcgI	CGANNNNNNTGC	2 cut(s)	836, 870
BciT130I	CCWGG	2 cut(s)	840, 903
BclI	TGATCA	1 cut(s)	622
BcnI	CCSGG	1 cut(s)	397
BcoDI	GTCTC	4 cut(s)	452, 848, 1216, 1244
BfaI	CTAG	1 cut(s)	485
BfmI	CTRYAG	2 cut(s)	602, 813
BfuAI	ACCTGC	1 cut(s)	806
BglII	AGATCT	1 cut(s)	305
BisI	GCNGC	4 cut(s)	586, 669, 813, 878
BlsI	GCNGC	4 cut(s)	587, 670, 814, 879
BmcAI	AGTACT	1 cut(s)	293
Bme1390I	CCNGG	3 cut(s)	397, 840, 903
Bme18I	GGWCC	1 cut(s)	754
BmgT120I	GGNCC	1 cut(s)	754
BmiI	GGNNCC	4 cut(s)	247, 714, 1077, 1125
BmrFI	CCNGG	3 cut(s)	397, 840, 903
BmsI	GCATC	4 cut(s)	243, 454, 881, 1078
BplI	GAGNNNNNCTC	2 cut(s)	1132, 1164
BpuEI	CTTGAG	1 cut(s)	254
BpuMI	CCSGG	1 cut(s)	397
Bsa29I	ATCGAT	1 cut(s)	18
BsaBI	GATNNNNATC	1 cut(s)	354
BsaI	GGTCTC	1 cut(s)	848
BsaJI	CCNNGG	4 cut(s)	9, 901, 902, 1096
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	3 cut(s)	182, 194, 637
Bse3DI	GCAATG	1 cut(s)	946
Bse8I	GATNNNNATC	1 cut(s)	354
BseBI	CCWGG	2 cut(s)	840, 903
BseCI	ATCGAT	1 cut(s)	18
BseDI	CCNNGG	4 cut(s)	9, 901, 902, 1096
BseJI	GATNNNNATC	1 cut(s)	354
BseMI	GCAATG	1 cut(s)	946
BseMII	CTCAG	3 cut(s)	762, 818, 1315
BseNI	ACTGG	3 cut(s)	182, 194, 637
BseRI	GAGGAG	1 cut(s)	146
BseSI	GKGCMC	1 cut(s)	345
BseXI	GCAGC	4 cut(s)	597, 680, 799, 889
BshNI	GGYRCC	1 cut(s)	1075
BshVI	ATCGAT	1 cut(s)	18
BsiHKAI	GWGCWC	1 cut(s)	345
BsiSI	CCGG	2 cut(s)	396, 468
BslFI	GGGAC	4 cut(s)	155, 236, 445, 1324
BsmAI	GTCTC	4 cut(s)	452, 848, 1216, 1244
BsmBI	CGTCTC	1 cut(s)	1244
BsmFI	GGGAC	4 cut(s)	155, 236, 445, 1324
BsmI	GAATGC	4 cut(s)	46, 236, 1233, 1295
Bso31I	GGTCTC	1 cut(s)	848
Bsp1286I	GDGCHC	1 cut(s)	345
Bsp1407I	TGTACA	1 cut(s)	96
Bsp143I	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
Bsp19I	CCATGG	1 cut(s)	9
BspACI	CCGC	1 cut(s)	51
BspCNI	CTCAG	3 cut(s)	763, 817, 1314
BspDI	ATCGAT	1 cut(s)	18
BspLI	GGNNCC	4 cut(s)	247, 714, 1077, 1125
BspMAI	CTGCAG	2 cut(s)	606, 817
BspMI	ACCTGC	1 cut(s)	806
BspPI	GGATC	3 cut(s)	1118, 1131, 1210
BspT107I	GGYRCC	1 cut(s)	1075
BspTNI	GGTCTC	1 cut(s)	848
BsrDI	GCAATG	1 cut(s)	946
BsrFI	RCCGGY	1 cut(s)	467
BsrGI	TGTACA	1 cut(s)	96
BsrI	ACTGG	3 cut(s)	182, 194, 637
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	4 cut(s)	9, 901, 902, 1096
BssMI	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
BssT1I	CCWWGG	2 cut(s)	9, 1096
Bst2UI	CCWGG	2 cut(s)	840, 903
Bst4CI	ACNGT	4 cut(s)	111, 205, 418, 436
Bst6I	CTCTTC	1 cut(s)	376
BstAUI	TGTACA	1 cut(s)	96
BstC8I	GCNNGC	1 cut(s)	502
BstDEI	CTNAG	6 cut(s)	297, 478, 771, 804, 1192, 1301
BstDSI	CCRYGG	1 cut(s)	9
BstKTI	GATC	8 cut(s)	308, 625, 932, 958, 1006, 1126, 1137, 1205
BstMAI	GTCTC	4 cut(s)	452, 848, 1216, 1244
BstMBI	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
BstMWI	GCNNNNNNNGC	4 cut(s)	610, 863, 877, 1082
BstNI	CCWGG	2 cut(s)	840, 903
BstSCI	CCNGG	3 cut(s)	395, 838, 901
BstSFI	CTRYAG	2 cut(s)	602, 813
BstSLI	GKGCMC	1 cut(s)	345
BstV1I	GCAGC	4 cut(s)	597, 680, 799, 889
BstX2I	RGATCY	3 cut(s)	305, 1123, 1202
BstYI	RGATCY	3 cut(s)	305, 1123, 1202
Bsu15I	ATCGAT	1 cut(s)	18
BsuTUI	ATCGAT	1 cut(s)	18
BtgI	CCRYGG	1 cut(s)	9
BtsIMutI	CAGTG	1 cut(s)	527
BveI	ACCTGC	1 cut(s)	806
Cac8I	GCNNGC	1 cut(s)	502
CaiI	CAGNNNCTG	1 cut(s)	434
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	1 cut(s)	754
ClaI	ATCGAT	1 cut(s)	18
CseI	GACGC	1 cut(s)	160
Csp6I	GTAC	4 cut(s)	97, 112, 292, 936
CviAII	CATG	7 cut(s)	10, 262, 449, 683, 719, 835, 933
CviQI	GTAC	4 cut(s)	97, 112, 292, 936
DdeI	CTNAG	6 cut(s)	297, 478, 771, 804, 1192, 1301
DpnI	GATC	8 cut(s)	307, 624, 931, 957, 1005, 1125, 1136, 1204
DpnII	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
DraI	TTTAAA	1 cut(s)	594
DrdI	GACNNNNNNGTC	1 cut(s)	479
DseDI	GACNNNNNNGTC	1 cut(s)	479
Eam1104I	CTCTTC	1 cut(s)	376
EarI	CTCTTC	1 cut(s)	376
EciI	GGCGGA	1 cut(s)	40
Eco130I	CCWWGG	2 cut(s)	9, 1096
Eco31I	GGTCTC	1 cut(s)	848
Eco32I	GATATC	2 cut(s)	793, 832
Eco47I	GGWCC	1 cut(s)	754
Eco57I	CTGAAG	1 cut(s)	1227
EcoRII	CCWGG	2 cut(s)	838, 901
EcoRV	GATATC	2 cut(s)	793, 832
EcoT14I	CCWWGG	2 cut(s)	9, 1096
EcoT22I	ATGCAT	1 cut(s)	236
ErhI	CCWWGG	2 cut(s)	9, 1096
Esp3I	CGTCTC	1 cut(s)	1244
FaeI	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
FalI	AAGNNNNNCTT	2 cut(s)	574, 606
FaqI	GGGAC	4 cut(s)	155, 236, 445, 1324
FatI	CATG	7 cut(s)	9, 261, 448, 682, 718, 834, 932
FauNDI	CATATG	1 cut(s)	141
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	1 cut(s)	136
Fnu4HI	GCNGC	4 cut(s)	586, 669, 813, 878
Fsp4HI	GCNGC	4 cut(s)	586, 669, 813, 878
FspBI	CTAG	1 cut(s)	485
GluI	GCNGC	4 cut(s)	586, 669, 813, 878
HapII	CCGG	2 cut(s)	396, 468
HgaI	GACGC	1 cut(s)	160
Hin1II	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
HincII	GTYRAC	2 cut(s)	107, 801
HindII	GTYRAC	2 cut(s)	107, 801
HinfI	GANTC	6 cut(s)	20, 82, 802, 1224, 1282, 1312
HpaII	CCGG	2 cut(s)	396, 468
HphI	GGTGA	3 cut(s)	402, 482, 1231
Hpy166II	GTNNAC	6 cut(s)	107, 137, 343, 446, 801, 1156
Hpy188I	TCNGA	7 cut(s)	25, 87, 360, 772, 807, 1021, 1207
Hpy8I	GTNNAC	6 cut(s)	107, 137, 343, 446, 801, 1156
HpyAV	CCTTC	5 cut(s)	82, 536, 646, 1021, 1202
HpyCH4III	ACNGT	4 cut(s)	111, 205, 418, 436
HpyCH4IV	ACGT	2 cut(s)	923, 1152
HpyCH4V	TGCA	5 cut(s)	234, 343, 604, 668, 815
HpyF10VI	GCNNNNNNNGC	4 cut(s)	610, 863, 877, 1082
HpyF3I	CTNAG	6 cut(s)	297, 478, 771, 804, 1192, 1301
HpySE526I	ACGT	2 cut(s)	923, 1152
Hsp92II	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
Ksp22I	TGATCA	1 cut(s)	622
Kzo9I	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
LmnI	GCTCC	1 cut(s)	159
Lsp1109I	GCAGC	4 cut(s)	597, 680, 799, 889
LweI	GCATC	4 cut(s)	243, 454, 881, 1078
MaeI	CTAG	1 cut(s)	485
MaeII	ACGT	2 cut(s)	923, 1152
MaeIII	GTNAC	3 cut(s)	310, 424, 470
MalI	GATC	8 cut(s)	307, 624, 931, 957, 1005, 1125, 1136, 1204
MboI	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
MboII	GAAGA	3 cut(s)	40, 380, 393
MflI	RGATCY	3 cut(s)	305, 1123, 1202
MhlI	GDGCHC	1 cut(s)	345
MlyI	GAGTC	3 cut(s)	796, 1218, 1306
MmeI	TCCRAC	3 cut(s)	223, 559, 768
Mph1103I	ATGCAT	1 cut(s)	236
MroXI	GAANNNNTTC	1 cut(s)	46
MseI	TTAA	6 cut(s)	387, 593, 729, 749, 780, 1271
MslI	CAYNNNNRTG	2 cut(s)	348, 1060
MspI	CCGG	2 cut(s)	396, 468
MspR9I	CCNGG	3 cut(s)	397, 840, 903
Mva1269I	GAATGC	4 cut(s)	46, 236, 1233, 1295
MvaI	CCWGG	2 cut(s)	840, 903
MwoI	GCNNNNNNNGC	4 cut(s)	610, 863, 877, 1082
NciI	CCSGG	1 cut(s)	397
NcoI	CCATGG	1 cut(s)	9
NdeI	CATATG	1 cut(s)	141
NdeII	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
NlaIII	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
NlaIV	GGNNCC	4 cut(s)	247, 714, 1077, 1125
NmuCI	GTSAC	2 cut(s)	310, 470
NsiI	ATGCAT	1 cut(s)	236
PasI	CCCWGGG	1 cut(s)	902
PctI	GAATGC	4 cut(s)	46, 236, 1233, 1295
PdmI	GAANNNNTTC	1 cut(s)	46
PfeI	GAWTC	3 cut(s)	20, 82, 1282
PflFI	GACNNNGTC	1 cut(s)	965
PfoI	TCCNGGA	1 cut(s)	395
PkrI	GCNGC	4 cut(s)	587, 670, 814, 879
PleI	GAGTC	3 cut(s)	796, 1218, 1306
PpsI	GAGTC	3 cut(s)	796, 1218, 1306
PsiI	TTATAA	1 cut(s)	918
Psp6I	CCWGG	2 cut(s)	838, 901
PspGI	CCWGG	2 cut(s)	838, 901
PspN4I	GGNNCC	4 cut(s)	247, 714, 1077, 1125
PspPI	GGNCC	1 cut(s)	754
PstI	CTGCAG	2 cut(s)	606, 817
PstNI	CAGNNNCTG	1 cut(s)	434
PsuI	RGATCY	3 cut(s)	305, 1123, 1202
PsyI	GACNNNGTC	1 cut(s)	965
RsaI	GTAC	4 cut(s)	98, 113, 293, 937
RsaNI	GTAC	4 cut(s)	97, 112, 292, 936
RseI	CAYNNNNRTG	2 cut(s)	348, 1060
SaqAI	TTAA	6 cut(s)	387, 593, 729, 749, 780, 1271
SatI	GCNGC	4 cut(s)	586, 669, 813, 878
Sau3AI	GATC	8 cut(s)	305, 622, 929, 955, 1003, 1123, 1134, 1202
Sau96I	GGNCC	1 cut(s)	754
ScaI	AGTACT	1 cut(s)	293
SchI	GAGTC	3 cut(s)	796, 1218, 1306
ScrFI	CCNGG	3 cut(s)	397, 840, 903
SduI	GDGCHC	1 cut(s)	345
SfaNI	GCATC	4 cut(s)	243, 454, 881, 1078
SfcI	CTRYAG	2 cut(s)	602, 813
SinI	GGWCC	1 cut(s)	754
SmiMI	CAYNNNNRTG	2 cut(s)	348, 1060
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
SsiI	CCGC	1 cut(s)	51
SspMI	CTAG	1 cut(s)	485
StyD4I	CCNGG	3 cut(s)	395, 838, 901
StyI	CCWWGG	2 cut(s)	9, 1096
TaaI	ACNGT	4 cut(s)	111, 205, 418, 436
TaiI	ACGT	2 cut(s)	926, 1155
TaqI	TCGA	3 cut(s)	18, 846, 1137
TatI	WGTACW	3 cut(s)	96, 111, 291
TfiI	GAWTC	3 cut(s)	20, 82, 1282
Tru1I	TTAA	6 cut(s)	387, 593, 729, 749, 780, 1271
Tru9I	TTAA	6 cut(s)	387, 593, 729, 749, 780, 1271
TscAI	CASTG	1 cut(s)	534
TseFI	GTSAC	2 cut(s)	310, 470
TseI	GCWGC	4 cut(s)	585, 668, 812, 877
Tsp45I	GTSAC	2 cut(s)	310, 470
TspDTI	ATGAA	3 cut(s)	278, 387, 671
TspGWI	ACGGA	2 cut(s)	122, 1130
TspRI	CASTG	1 cut(s)	534
Tth111I	GACNNNGTC	1 cut(s)	965
VneI	GTGCAC	1 cut(s)	341
VpaK11BI	GGWCC	1 cut(s)	754
XapI	RAATTY	3 cut(s)	700, 739, 1241
XmiI	GTMKAC	1 cut(s)	136
XmnI	GAANNNNTTC	1 cut(s)	46
XspI	CTAG	1 cut(s)	485
ZrmI	AGTACT	1 cut(s)	293
Zsp2I	ATGCAT	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.