Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009156

Physical Location & Seq

Genomic Coordinates Reverse (-)

5958469 .. 5963962

Gene Structure 5494 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1057800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGAAGCCATGGAAATCGATTCGGAGAAGAACCTCCACAGAAAGCATTCCACCTACAAATTGCTGGACGAGAGGTTTGGGATTCAGAAGGAAATGTACAGGGGTCAACAGTACAGCCAAATATACTTCGCCCGCCTCCATATGATGAGGACCCTTCTCTATTCCCTTGTCCCTAACTGGAAACCCCATTTGCCAGTTTGCAGAGTTTTGGGATCGGAAAGGGACAAGGAATGCATCATTGTTGGAACCCTCTACAAGCACATGAAACTCAAGCCTTGTGTTCTTGATGAATACTCTAAGACGAGAACTGTGACACCACTTGTCAAGCCTCATAATTTTGTGCACCCAGATGACCATCTGATTTTGGAAGATGAAAGTGGAAGAGTTAAGCTTTCTGGGAATGTGCTTTCACCTTCTGTGTATGTAACAGGGACTATTGTTGGTTTACATGGAAAGGAGACTGATGCCGGTGACTTCTTTGTCCTAGATGTTCTTGAAGCTGGATTACCACCCCAGATAGAGTTGCCACTTAAATCAAGAGAAGGCAAATATGTTGTTTTTGTATCTGGACTTCGTGTCGGAAGCAGCTCTTTAAACCCTCTCCAGTTTCAGCTTCTCGTTGATCATATAACAGGACATTTAGGAGATGAGAAGGAACAAGGTATTGCAGCACAGATAGTTCATGTTGTAATTGCTGGAAATTCTATTGAGGTTCCTCATGGACTTCTTAATGGACAGAATTTAGCGTCAAAGGATCAATCAAGGCTGTCTGAGCCAATTAAGGAGCTGGATATTTTGTTGACTCAGATTGCTGCAGGTTTGTCTTTGGATATCATGCCTGGTATCTATGACCCTGCTAATTTTGCCTTGCCACAGCAGCCTTTGAACAGATGCCTTTTCCCTGGTTCATCAGCTTATAACACGTTCAGATCATGTACCAATCCCCATTGCTTTGATCTTGATGATGTCAGGTTTCTTGGAACATCGGGGCAAAATATAGATGATCTTGATAAGTATTCAGAAGCAACGGATAAACTTGACTTTATTGAAAGGACATTGAGGTGGAGGCATCTGGCACCAACAGCACCTAATACTCTTGGGTGCTATCCTTTCACGGATAGGGATCCGTTCCTGATCGAGAGTTGTCCTCACGTTTACTTTGTTGGTAATCAGGATAAATATGATAGTCGCTTAGTAAAGGGATCTGATGGTCAGATGGTGAGACTCATTAGCATACCTAAATTCTGTGAGACGGGAGTTGCTGTTGTGCTAAACTTGGGGAATCTTGAATGCCATACACTCAGTTTCGGGACTCAGCTTAGGCTTCTGAAGACATCCTCTAACAGCATTTGTGGAGATCCATTGAAAAGAAATCCGAATGGATGGAATCTGCGGAGGCAGTATATTTGTTTCTTATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006271 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0022616 GO:0030894 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0033554 GO:0034641 GO:0034645 GO:0042575 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0043625 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0061695 GO:0070013 GO:0071704 GO:0090304 GO:1901360 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 ko03410 ko03420 ko03430 ko03440 ko05166 map00230 map00240 map01100 map03030 map03410 map03420 map03430 map03440 map05166

Pfam Domains

Protein Families

DNA_pol_E_B

Protein Analysis

474

Amino Acids

53.18

Weight (kDa)

6.5

Isoelectric Point (pI)

37.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_D_N	PF18018	37 - 164	2.9e-39	DNA polymerase delta subunit OB-fold domain
DNA_pol_E_B	PF04042	185 - 394	2e-52	DNA polymerase alpha/epsilon subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G42120 AT2G42120
fragaria_vesca	FvH4_6g22330 FvH4_6g22330
malus_domestica	MD12G1057200.v1.1 MD14G1057800.v1.1
prunus_persica	Prupe.7G076900_v2.0.a1 Prupe.7G076900_v2.0.a1 Prupe.7G076900_v2.0.a1
pyrus_communis	pycom14g04690
rosa_chinensis	RchiOBHm_Chr3g0479691
rosa_multiflora	Rmu_sc0003755.1_g000006 Rmu_sc0031395.1_g000006
rosa_roxburghii	Rroxscaffold_6G00402060
rosa_rugosa	Rorug03G0177400 Rorug03G0177500 Rorug03G0177600
rosa_samantha	Rh3AG228200 Rh3BG261400 Rh3CG258500 Rh3DG254700
rosa_wichuraiana	Rw3G020420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	918
AasI	GACNNNNNNGTC	1 cut(s)	479
Acc36I	ACCTGC	1 cut(s)	806
AccB1I	GGYRCC	1 cut(s)	1075
AciI	CCGC	2 cut(s)	133, 1393
AclWI	GGATC	6 cut(s)	220, 762, 1118, 1131, 1210, 1352
AcsI	RAATTY	3 cut(s)	700, 739, 1241
AcuI	CTGAAG	1 cut(s)	1349
AfaI	GTAC	3 cut(s)	98, 113, 937
AflIII	ACRYGT	1 cut(s)	921
AgsI	TTSAA	5 cut(s)	497, 886, 1049, 1289, 1366
AhdI	GACNNNNNGTC	1 cut(s)	575
AjnI	CCWGG	2 cut(s)	838, 901
AluBI	AGCT	7 cut(s)	391, 500, 588, 613, 787, 914, 1318
AluI	AGCT	7 cut(s)	391, 500, 588, 613, 787, 914, 1318
Alw21I	GWGCWC	1 cut(s)	345
Alw26I	GTCTC	3 cut(s)	452, 1216, 1244
Alw44I	GTGCAC	1 cut(s)	341
AlwI	GGATC	6 cut(s)	220, 762, 1118, 1131, 1210, 1352
ApaLI	GTGCAC	1 cut(s)	341
ApeKI	GCWGC	4 cut(s)	585, 668, 812, 877
ApoI	RAATTY	3 cut(s)	700, 739, 1241
ArsI	GACNNNNNNTTYG	2 cut(s)	59, 91
Asp700I	GAANNNNTTC	1 cut(s)	46
AspS9I	GGNCC	1 cut(s)	150
AsuHPI	GGTGA	3 cut(s)	402, 482, 1231
AvaII	GGWCC	1 cut(s)	150
BaeGI	GKGCMC	1 cut(s)	345
BamHI	GGATCC	1 cut(s)	1123
BanI	GGYRCC	1 cut(s)	1075
BarI	GAAGNNNNNNTAC	6 cut(s)	80, 112, 489, 521, 555, 587
BbsI	GAAGAC	1 cut(s)	1337
Bbv12I	GWGCWC	1 cut(s)	345
BbvI	GCAGC	4 cut(s)	597, 680, 799, 889
BccI	CCATC	4 cut(s)	363, 1202, 1210, 1377
BciT130I	CCWGG	2 cut(s)	840, 903
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	3 cut(s)	452, 1216, 1244
BfaI	CTAG	1 cut(s)	485
BfmI	CTRYAG	1 cut(s)	813
BfuAI	ACCTGC	1 cut(s)	806
BisI	GCNGC	4 cut(s)	586, 669, 813, 878
BlsI	GCNGC	4 cut(s)	587, 670, 814, 879
Bme1390I	CCNGG	2 cut(s)	840, 903
Bme18I	GGWCC	1 cut(s)	150
BmeRI	GACNNNNNGTC	1 cut(s)	575
BmgT120I	GGNCC	1 cut(s)	150
BmiI	GGNNCC	5 cut(s)	152, 247, 714, 1077, 1125
BmrFI	CCNGG	2 cut(s)	840, 903
BmsI	GCATC	4 cut(s)	243, 454, 881, 1078
BpiI	GAAGAC	1 cut(s)	1337
BplI	GAGNNNNNCTC	2 cut(s)	1132, 1164
BpmI	CTGGAG	1 cut(s)	587
Bpu10I	CCTNAGC	1 cut(s)	1319
BpuEI	CTTGAG	1 cut(s)	254
Bsa29I	ATCGAT	1 cut(s)	18
BsaBI	GATNNNNATC	2 cut(s)	354, 1122
BsaJI	CCNNGG	2 cut(s)	9, 901
BsaXI	ACNNNNNCTCC	2 cut(s)	1248, 1278
Bse118I	RCCGGY	1 cut(s)	467
Bse1I	ACTGG	3 cut(s)	182, 194, 604
Bse3DI	GCAATG	1 cut(s)	946
Bse8I	GATNNNNATC	2 cut(s)	354, 1122
BseBI	CCWGG	2 cut(s)	840, 903
BseCI	ATCGAT	1 cut(s)	18
BseDI	CCNNGG	2 cut(s)	9, 901
BseGI	GGATG	2 cut(s)	1334, 1388
BseJI	GATNNNNATC	2 cut(s)	354, 1122
BseMI	GCAATG	1 cut(s)	946
BseMII	CTCAG	4 cut(s)	762, 818, 1315, 1328
BseNI	ACTGG	3 cut(s)	182, 194, 604
BseSI	GKGCMC	1 cut(s)	345
BseXI	GCAGC	4 cut(s)	597, 680, 799, 889
BshNI	GGYRCC	1 cut(s)	1075
BshVI	ATCGAT	1 cut(s)	18
BsiHKAI	GWGCWC	1 cut(s)	345
BsiSI	CCGG	1 cut(s)	468
BslFI	GGGAC	4 cut(s)	155, 236, 445, 1324
BsmAI	GTCTC	3 cut(s)	452, 1216, 1244
BsmBI	CGTCTC	1 cut(s)	1244
BsmFI	GGGAC	4 cut(s)	155, 236, 445, 1324
BsmI	GAATGC	3 cut(s)	46, 236, 1295
Bsp1286I	GDGCHC	1 cut(s)	345
Bsp1407I	TGTACA	1 cut(s)	96
Bsp19I	CCATGG	1 cut(s)	9
BspACI	CCGC	2 cut(s)	133, 1393
BspCNI	CTCAG	4 cut(s)	763, 817, 1314, 1327
BspDI	ATCGAT	1 cut(s)	18
BspLI	GGNNCC	5 cut(s)	152, 247, 714, 1077, 1125
BspMAI	CTGCAG	1 cut(s)	817
BspMI	ACCTGC	1 cut(s)	806
BspPI	GGATC	6 cut(s)	220, 762, 1118, 1131, 1210, 1352
BspT107I	GGYRCC	1 cut(s)	1075
BsrDI	GCAATG	1 cut(s)	946
BsrFI	RCCGGY	1 cut(s)	467
BsrGI	TGTACA	1 cut(s)	96
BsrI	ACTGG	3 cut(s)	182, 194, 604
BssAI	RCCGGY	1 cut(s)	467
BssECI	CCNNGG	2 cut(s)	9, 901
BssT1I	CCWWGG	1 cut(s)	9
Bst2UI	CCWGG	2 cut(s)	840, 903
Bst4CI	ACNGT	2 cut(s)	111, 310
Bst6I	CTCTTC	1 cut(s)	376
BstAUI	TGTACA	1 cut(s)	96
BstC8I	GCNNGC	1 cut(s)	133
BstDEI	CTNAG	7 cut(s)	297, 771, 804, 1192, 1301, 1314, 1319
BstDSI	CCRYGG	1 cut(s)	9
BstF5I	GGATG	2 cut(s)	1334, 1388
BstMAI	GTCTC	3 cut(s)	452, 1216, 1244
BstMWI	GCNNNNNNNGC	4 cut(s)	772, 863, 877, 1082
BstNI	CCWGG	2 cut(s)	840, 903
BstSCI	CCNGG	2 cut(s)	838, 901
BstSFI	CTRYAG	1 cut(s)	813
BstSLI	GKGCMC	1 cut(s)	345
BstV1I	GCAGC	4 cut(s)	597, 680, 799, 889
BstV2I	GAAGAC	1 cut(s)	1337
BstX2I	RGATCY	3 cut(s)	1123, 1202, 1357
BstYI	RGATCY	3 cut(s)	1123, 1202, 1357
Bsu15I	ATCGAT	1 cut(s)	18
BsuTUI	ATCGAT	1 cut(s)	18
BtgI	CCRYGG	1 cut(s)	9
BtsCI	GGATG	2 cut(s)	1334, 1388
BveI	ACCTGC	1 cut(s)	806
Cac8I	GCNNGC	1 cut(s)	133
Cfr10I	RCCGGY	1 cut(s)	467
Cfr13I	GGNCC	1 cut(s)	150
ClaI	ATCGAT	1 cut(s)	18
CseI	GACGC	1 cut(s)	735
Csp6I	GTAC	3 cut(s)	97, 112, 936
CviAII	CATG	7 cut(s)	10, 262, 449, 683, 719, 835, 933
CviQI	GTAC	3 cut(s)	97, 112, 936
DdeI	CTNAG	7 cut(s)	297, 771, 804, 1192, 1301, 1314, 1319
DraI	TTTAAA	1 cut(s)	594
DrdI	GACNNNNNNGTC	1 cut(s)	479
DriI	GACNNNNNGTC	1 cut(s)	575
DseDI	GACNNNNNNGTC	1 cut(s)	479
Eam1104I	CTCTTC	1 cut(s)	376
Eam1105I	GACNNNNNGTC	1 cut(s)	575
EarI	CTCTTC	1 cut(s)	376
Eco130I	CCWWGG	1 cut(s)	9
Eco32I	GATATC	1 cut(s)	832
Eco47I	GGWCC	1 cut(s)	150
Eco57I	CTGAAG	1 cut(s)	1349
EcoO109I	RGGNCCY	1 cut(s)	150
EcoRII	CCWGG	2 cut(s)	838, 901
EcoRV	GATATC	1 cut(s)	832
EcoT14I	CCWWGG	1 cut(s)	9
EcoT22I	ATGCAT	1 cut(s)	236
ErhI	CCWWGG	1 cut(s)	9
Esp3I	CGTCTC	1 cut(s)	1244
FaeI	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
FalI	AAGNNNNNCTT	2 cut(s)	574, 606
FaqI	GGGAC	4 cut(s)	155, 236, 445, 1324
FatI	CATG	7 cut(s)	9, 261, 448, 682, 718, 834, 932
FauI	CCCGC	1 cut(s)	140
FauNDI	CATATG	1 cut(s)	141
FbaI	TGATCA	1 cut(s)	622
Fnu4HI	GCNGC	4 cut(s)	586, 669, 813, 878
FokI	GGATG	2 cut(s)	1321, 1395
Fsp4HI	GCNGC	4 cut(s)	586, 669, 813, 878
FspBI	CTAG	1 cut(s)	485
GluI	GCNGC	4 cut(s)	586, 669, 813, 878
GsuI	CTGGAG	1 cut(s)	587
HapII	CCGG	1 cut(s)	468
HgaI	GACGC	1 cut(s)	735
Hin1II	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
HincII	GTYRAC	2 cut(s)	107, 801
HindII	GTYRAC	2 cut(s)	107, 801
HindIII	AAGCTT	1 cut(s)	389
HinfI	GANTC	7 cut(s)	20, 82, 802, 1224, 1282, 1312, 1387
HpaII	CCGG	1 cut(s)	468
HphI	GGTGA	3 cut(s)	402, 482, 1231
Hpy166II	GTNNAC	5 cut(s)	107, 343, 446, 801, 1156
Hpy8I	GTNNAC	5 cut(s)	107, 343, 446, 801, 1156
HpyAV	CCTTC	5 cut(s)	82, 164, 423, 536, 646
HpyCH4III	ACNGT	2 cut(s)	111, 310
HpyCH4IV	ACGT	2 cut(s)	923, 1152
HpyCH4V	TGCA	5 cut(s)	201, 234, 343, 668, 815
HpyF10VI	GCNNNNNNNGC	4 cut(s)	772, 863, 877, 1082
HpyF3I	CTNAG	7 cut(s)	297, 771, 804, 1192, 1301, 1314, 1319
HpySE526I	ACGT	2 cut(s)	923, 1152
Hsp92II	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
Ksp22I	TGATCA	1 cut(s)	622
LmnI	GCTCC	1 cut(s)	784
Lsp1109I	GCAGC	4 cut(s)	597, 680, 799, 889
LweI	GCATC	4 cut(s)	243, 454, 881, 1078
MaeI	CTAG	1 cut(s)	485
MaeII	ACGT	2 cut(s)	923, 1152
MaeIII	GTNAC	3 cut(s)	310, 424, 470
MboII	GAAGA	4 cut(s)	40, 380, 393, 1342
MflI	RGATCY	3 cut(s)	1123, 1202, 1357
MhlI	GDGCHC	1 cut(s)	345
MluCI	AATT	8 cut(s)	59, 334, 690, 700, 739, 777, 859, 1241
MlyI	GAGTC	3 cut(s)	796, 1218, 1306
MmeI	TCCRAC	2 cut(s)	223, 559
Mph1103I	ATGCAT	1 cut(s)	236
MroXI	GAANNNNTTC	1 cut(s)	46
MseI	TTAA	5 cut(s)	387, 531, 593, 729, 780
MslI	CAYNNNNRTG	2 cut(s)	348, 1060
MspI	CCGG	1 cut(s)	468
MspR9I	CCNGG	2 cut(s)	840, 903
Mva1269I	GAATGC	3 cut(s)	46, 236, 1295
MvaI	CCWGG	2 cut(s)	840, 903
MwoI	GCNNNNNNNGC	4 cut(s)	772, 863, 877, 1082
NcoI	CCATGG	1 cut(s)	9
NdeI	CATATG	1 cut(s)	141
NlaIII	CATG	7 cut(s)	13, 265, 452, 686, 722, 838, 936
NlaIV	GGNNCC	5 cut(s)	152, 247, 714, 1077, 1125
NmuCI	GTSAC	2 cut(s)	310, 470
NsiI	ATGCAT	1 cut(s)	236
PctI	GAATGC	3 cut(s)	46, 236, 1295
PdmI	GAANNNNTTC	1 cut(s)	46
PfeI	GAWTC	4 cut(s)	20, 82, 1282, 1387
PkrI	GCNGC	4 cut(s)	587, 670, 814, 879
PleI	GAGTC	3 cut(s)	796, 1218, 1306
PpsI	GAGTC	3 cut(s)	796, 1218, 1306
PpuMI	RGGWCCY	1 cut(s)	150
PsiI	TTATAA	1 cut(s)	918
Psp5II	RGGWCCY	1 cut(s)	150
Psp6I	CCWGG	2 cut(s)	838, 901
PspGI	CCWGG	2 cut(s)	838, 901
PspN4I	GGNNCC	5 cut(s)	152, 247, 714, 1077, 1125
PspPI	GGNCC	1 cut(s)	150
PspPPI	RGGWCCY	1 cut(s)	150
PstI	CTGCAG	1 cut(s)	817
PsuI	RGATCY	3 cut(s)	1123, 1202, 1357
RsaI	GTAC	3 cut(s)	98, 113, 937
RsaNI	GTAC	3 cut(s)	97, 112, 936
RseI	CAYNNNNRTG	2 cut(s)	348, 1060
SaqAI	TTAA	5 cut(s)	387, 531, 593, 729, 780
SatI	GCNGC	4 cut(s)	586, 669, 813, 878
Sau96I	GGNCC	1 cut(s)	150
SchI	GAGTC	3 cut(s)	796, 1218, 1306
ScrFI	CCNGG	2 cut(s)	840, 903
SduI	GDGCHC	1 cut(s)	345
SfaNI	GCATC	4 cut(s)	243, 454, 881, 1078
SfcI	CTRYAG	1 cut(s)	813
SinI	GGWCC	1 cut(s)	150
SmiMI	CAYNNNNRTG	2 cut(s)	348, 1060
SmlI	CTYRAG	1 cut(s)	269
SmoI	CTYRAG	1 cut(s)	269
Sse9I	AATT	8 cut(s)	59, 334, 690, 700, 739, 777, 859, 1241
SsiI	CCGC	2 cut(s)	133, 1393
SspMI	CTAG	1 cut(s)	485
StyD4I	CCNGG	2 cut(s)	838, 901
StyI	CCWWGG	1 cut(s)	9
TaaI	ACNGT	2 cut(s)	111, 310
TaiI	ACGT	2 cut(s)	926, 1155
TaqI	TCGA	2 cut(s)	18, 1137
TasI	AATT	8 cut(s)	59, 334, 690, 700, 739, 777, 859, 1241
TatI	WGTACW	2 cut(s)	96, 111
TfiI	GAWTC	4 cut(s)	20, 82, 1282, 1387
Tru1I	TTAA	5 cut(s)	387, 531, 593, 729, 780
Tru9I	TTAA	5 cut(s)	387, 531, 593, 729, 780
TseFI	GTSAC	2 cut(s)	310, 470
TseI	GCWGC	4 cut(s)	585, 668, 812, 877
Tsp45I	GTSAC	2 cut(s)	310, 470
TspDTI	ATGAA	5 cut(s)	278, 303, 387, 671, 897
TspGWI	ACGGA	3 cut(s)	1043, 1116, 1130
VneI	GTGCAC	1 cut(s)	341
VpaK11BI	GGWCC	1 cut(s)	150
XapI	RAATTY	3 cut(s)	700, 739, 1241
XmnI	GAANNNNTTC	1 cut(s)	46
XspI	CTAG	1 cut(s)	485
Zsp2I	ATGCAT	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.