MD12G1059200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013312
Physical Location & Seq
Reverse (-)
6901052 .. 6902071
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1059200.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGAAAGCGTCGGTCAAATTCCGGGAGGACCAGAAGCCGTTGCTGAGGGCCAAGGTGCCCCTCAGCATCCTGGGCCTGCCCTTCCAGTCCGGAATCGTCGCCGGTGAGTCCAAGGAGCTCACTCTCAACCTCGCCACCTTCTTCGAATCCGGGCCGTCCATCAAGATCGCGTATCGTCCCAACGACGCGTGGAACCCCTTCTCCCTCGTCGTCAAGACCGGGACGGGATCCTTCGGATCGCCGATTTCGAGCTCCATGCTGATGAGCGCCGAGTTCAATCTCCTTGACCGCGGGAGAAGCCCTAGCTTCATGCTCCACTTTAAGCCCCAGTTCGGCGATTTCTCGATCAAGAAGACGCAGTCCTCGGTCTTCGAGAAGATCGTGGGTTCTGAGAAGGACGCCGTTGCGGCGGAGACGCCGGTAGTGGAGGCGGAGTTTTCGGGGAAGAGAAAAGTTTTGTCATCGGCAAGTCTGGCGGCTGGAGTTCTTGCCCGTTTGTTCACCGGGATGGAGGTGGCGGCGAGGACGTCGATGCCGGTGAGGAACCGGGCCGTGGTGAACTTGAGGTGGGGGGTTAAGGTTCCGACGGAGATAAAGAGTGCTGGGGTGAATCCGACTGCGGGGATTGCGTTTCAGAAAATCCCGTTCCTGGTGATGAACAAGATCGGGATCGAACACGTGGACGGTGGCGATTCGAAGGCCAAAACCACAAAGGCGGCGGCGGATAATGCGTTGACATTTCCGGGAAATGCTGAGGTTGCGGAGGCTTGTTTTAGGGTGAAGCATCAGCTGGAGGCTCTGCGGACGGAGAACGGGTTGCTGAGAAAATCCGTGGAGGATCTGCGCCACGAAATCGCCGGCGCCGCGAAGTCGATTTCGATTTCGAATGCGTTTCCTGAGAGTGAGATAAATGGGGGAAGTAGGAATTCGAGCGGGAAAGTGGATCGGCGATCAAACGGGAAGAATAAGTCGCCGGAGGGCGATGTGGCGGAGGAACTGAAGAAGGCCCTCAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

36.38

Weight (kDa)

9.7

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57990
fragaria_vesca FvH4_6g22460
malus_domestica MD12G1059200.v1.1 MD14G1058800.v1.1
prunus_persica Prupe.7G076000_v2.0.a1
pyrus_communis pycom12g05180 pycom14g04760
rosa_chinensis RchiOBHm_Chr3g0479871
rosa_laevigata RLG00000023529
rosa_multiflora Rmu_co8502759.1_g000001
rosa_roxburghii Rroxscaffold_6G00401940
rosa_rugosa Rorug03G0179100
rosa_samantha Rh3AG230000 Rh3BG262700 Rh3CG260000 Rh3DG256100
rosa_wichuraiana Rw0G015260 Rw0G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 530
AccB1I GGYRCC 2 cut(s) 55, 860
AccBSI CCGCTC 1 cut(s) 933
AccII CGCG 4 cut(s) 170, 188, 291, 866
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 6 cut(s) 222, 235, 244, 677, 846, 951
AcsI RAATTY 2 cut(s) 17, 925
AcuI CTGAAG 1 cut(s) 1019
AcvI CACGTG 1 cut(s) 679
AcyI GRCGYC 4 cut(s) 399, 416, 527, 861
AfiI CCNNNNNNNGG 4 cut(s) 332, 553, 620, 649
AflIII ACRYGT 2 cut(s) 186, 676
AgsI TTSAA 1 cut(s) 277
AjnI CCWGG 2 cut(s) 69, 648
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 118, 252, 306, 790
AluI AGCT 4 cut(s) 118, 252, 306, 790
Alw21I GWGCWC 2 cut(s) 120, 254
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 6 cut(s) 222, 235, 244, 677, 846, 951
AlwNI CAGNNNCTG 1 cut(s) 1017
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 6 cut(s) 48, 73, 152, 549, 699, 1005
ApoI RAATTY 2 cut(s) 17, 925
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 269, 846, 863
AspS9I GGNCC 6 cut(s) 28, 48, 73, 152, 549, 1006
AsuC2I CCSGG 6 cut(s) 23, 151, 220, 505, 548, 744
AsuHPI GGTGA 7 cut(s) 116, 493, 550, 568, 619, 664, 790
AsuII TTCGAA 3 cut(s) 144, 695, 884
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 60
BamHI GGATCC 1 cut(s) 227
BanI GGYRCC 2 cut(s) 55, 860
BanII GRGCYC 2 cut(s) 120, 254
BbrPI CACGTG 1 cut(s) 679
BbsI GAAGAC 2 cut(s) 359, 361
Bbv12I GWGCWC 2 cut(s) 120, 254
BbvCI CCTCAGC 3 cut(s) 44, 62, 753
BccI CCATC 2 cut(s) 167, 502
BceAI ACGGC 4 cut(s) 22, 139, 386, 536
BcgI CGANNNNNNTGC 2 cut(s) 238, 272
BciT130I CCWGG 2 cut(s) 71, 650
BcnI CCSGG 6 cut(s) 23, 151, 220, 505, 548, 744
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 270, 864
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 6 cut(s) 408, 477, 519, 717, 720, 864
BlsI GCNGC 6 cut(s) 409, 478, 520, 718, 721, 865
Bme1390I CCNGG 8 cut(s) 23, 71, 151, 220, 505, 548, 650, 744
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 6 cut(s) 28, 48, 73, 152, 549, 1006
BmiI GGNNCC 6 cut(s) 57, 194, 229, 545, 582, 862
BmrFI CCNGG 8 cut(s) 23, 71, 151, 220, 505, 548, 650, 744
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 3 cut(s) 75, 522, 793
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 359, 361
BpmI CTGGAG 2 cut(s) 501, 812
Bpu10I CCTNAGC 3 cut(s) 44, 62, 753
Bpu14I TTCGAA 3 cut(s) 144, 695, 884
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 6 cut(s) 23, 151, 220, 505, 548, 744
BsaAI YACGTR 1 cut(s) 679
BsaBI GATNNNNATC 1 cut(s) 668
BsaHI GRCGYC 4 cut(s) 399, 416, 527, 861
BsaJI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 552, 831
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 419, 449, 800, 830
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 553, 620, 649
Bse118I RCCGGY 4 cut(s) 101, 418, 535, 857
Bse1I ACTGG 2 cut(s) 85, 328
Bse8I GATNNNNATC 1 cut(s) 668
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 2 cut(s) 71, 650
BseDI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 552, 831
BseGI GGATG 2 cut(s) 66, 513
BseJI GATNNNNATC 1 cut(s) 668
BseLI CCNNNNNNNGG 4 cut(s) 332, 553, 620, 649
BseMII CTCAG 6 cut(s) 35, 76, 381, 744, 812, 888
BseNI ACTGG 2 cut(s) 85, 328
BseSI GKGCMC 1 cut(s) 60
BseYI CCCAGC 1 cut(s) 602
Bsh1236I CGCG 4 cut(s) 170, 188, 291, 866
BshFI GGCC 6 cut(s) 50, 75, 154, 551, 701, 1007
BshNI GGYRCC 2 cut(s) 55, 860
BsiHKAI GWGCWC 2 cut(s) 120, 254
BslFI GGGAC 2 cut(s) 162, 235
BslI CCNNNNNNNGG 4 cut(s) 332, 553, 620, 649
BsmAI GTCTC 1 cut(s) 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 162, 235
BsmI GAATGC 1 cut(s) 892
BsnI GGCC 6 cut(s) 50, 75, 154, 551, 701, 1007
Bsp119I TTCGAA 3 cut(s) 144, 695, 884
Bsp1286I GDGCHC 3 cut(s) 60, 120, 254
Bsp13I TCCGGA 1 cut(s) 89
BspANI GGCC 6 cut(s) 50, 75, 154, 551, 701, 1007
BspCNI CTCAG 6 cut(s) 36, 75, 382, 745, 813, 889
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 4 cut(s) 170, 188, 291, 866
BspLI GGNNCC 6 cut(s) 57, 194, 229, 545, 582, 862
BspPI GGATC 6 cut(s) 222, 235, 244, 677, 846, 951
BspT104I TTCGAA 3 cut(s) 144, 695, 884
BspT107I GGYRCC 2 cut(s) 55, 860
BsrBI CCGCTC 1 cut(s) 933
BsrFI RCCGGY 4 cut(s) 101, 418, 535, 857
BsrI ACTGG 2 cut(s) 85, 328
BssAI RCCGGY 4 cut(s) 101, 418, 535, 857
BssECI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 552, 831
BssNI GRCGYC 4 cut(s) 399, 416, 527, 861
BssT1I CCWWGG 2 cut(s) 51, 111
Bst2UI CCWGG 2 cut(s) 71, 650
Bst4CI ACNGT 1 cut(s) 686
Bst6I CTCTTC 1 cut(s) 440
BstACI GRCGYC 4 cut(s) 399, 416, 527, 861
BstBAI YACGTR 1 cut(s) 679
BstBI TTCGAA 3 cut(s) 144, 695, 884
BstC8I GCNNGC 2 cut(s) 77, 859
BstDEI CTNAG 7 cut(s) 44, 62, 390, 753, 821, 897, 1010
BstDSI CCRYGG 3 cut(s) 289, 552, 831
BstF5I GGATG 2 cut(s) 66, 513
BstFNI CGCG 4 cut(s) 170, 188, 291, 866
BstH2I RGCGCY 2 cut(s) 270, 864
BstHHI GCGC 3 cut(s) 269, 846, 863
BstMAI GTCTC 1 cut(s) 407
BstMWI GCNNNNNNNGC 8 cut(s) 72, 297, 407, 473, 626, 728, 758, 863
BstNI CCWGG 2 cut(s) 71, 650
BstSCI CCNGG 8 cut(s) 21, 69, 149, 218, 503, 546, 648, 742
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 4 cut(s) 170, 188, 291, 866
BstV2I GAAGAC 2 cut(s) 359, 361
BstX2I RGATCY 2 cut(s) 227, 838
BstYI RGATCY 2 cut(s) 227, 838
BsuRI GGCC 6 cut(s) 50, 75, 154, 551, 701, 1007
BtgI CCRYGG 3 cut(s) 289, 552, 831
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 2 cut(s) 66, 513
BtsIMutI CAGTG 1 cut(s) 1015
Cac8I GCNNGC 2 cut(s) 77, 859
CaiI CAGNNNCTG 1 cut(s) 1017
CfoI GCGC 3 cut(s) 269, 846, 863
Cfr10I RCCGGY 4 cut(s) 101, 418, 535, 857
Cfr13I GGNCC 6 cut(s) 28, 48, 73, 152, 549, 1006
Cfr42I CCGCGG 1 cut(s) 292
CseI GACGC 4 cut(s) 194, 364, 407, 424
CviAII CATG 2 cut(s) 256, 310
DdeI CTNAG 7 cut(s) 44, 62, 390, 753, 821, 897, 1010
DinI GGCGCC 1 cut(s) 862
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
EciI GGCGGA 4 cut(s) 425, 446, 737, 1004
Ecl136II GAGCTC 2 cut(s) 118, 252
Eco130I CCWWGG 2 cut(s) 51, 111
Eco24I GRGCYC 2 cut(s) 120, 254
Eco47I GGWCC 1 cut(s) 28
Eco53kI GAGCTC 2 cut(s) 118, 252
Eco57I CTGAAG 1 cut(s) 1019
Eco72I CACGTG 1 cut(s) 679
EcoICRI GAGCTC 2 cut(s) 118, 252
EcoO109I RGGNCCY 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 925
EcoRII CCWGG 2 cut(s) 69, 648
EcoT14I CCWWGG 2 cut(s) 51, 111
EcoT38I GRGCYC 2 cut(s) 120, 254
EgeI GGCGCC 1 cut(s) 862
EheI GGCGCC 1 cut(s) 862
ErhI CCWWGG 2 cut(s) 51, 111
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 259, 313
FaiI YATR 2 cut(s) 257, 311
FaqI GGGAC 2 cut(s) 162, 235
FatI CATG 2 cut(s) 255, 309
FauI CCCGC 3 cut(s) 284, 613, 926
Fnu4HI GCNGC 6 cut(s) 408, 477, 519, 717, 720, 864
FokI GGATG 2 cut(s) 53, 520
FriOI GRGCYC 2 cut(s) 120, 254
Fsp4HI GCNGC 6 cut(s) 408, 477, 519, 717, 720, 864
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 268, 845, 862
GluI GCNGC 6 cut(s) 408, 477, 519, 717, 720, 864
GsaI CCCAGC 1 cut(s) 606
GsuI CTGGAG 2 cut(s) 501, 812
HaeII RGCGCY 2 cut(s) 270, 864
HaeIII GGCC 6 cut(s) 50, 75, 154, 551, 701, 1007
HgaI GACGC 4 cut(s) 194, 364, 407, 424
HhaI GCGC 3 cut(s) 269, 846, 863
Hin1I GRCGYC 4 cut(s) 399, 416, 527, 861
Hin1II CATG 2 cut(s) 259, 313
Hin6I GCGC 3 cut(s) 267, 844, 861
HinP1I GCGC 3 cut(s) 267, 844, 861
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 5 cut(s) 93, 107, 146, 610, 692
HphI GGTGA 7 cut(s) 116, 493, 550, 568, 619, 664, 790
Hpy166II GTNNAC 4 cut(s) 501, 559, 682, 735
Hpy188I TCNGA 5 cut(s) 236, 391, 585, 615, 636
Hpy188III TCNNGA 8 cut(s) 90, 163, 214, 343, 349, 373, 667, 896
Hpy8I GTNNAC 4 cut(s) 501, 559, 682, 735
Hpy99I CGWCG 6 cut(s) 13, 101, 188, 212, 532, 589
HpyAV CCTTC 7 cut(s) 91, 148, 208, 241, 388, 691, 997
HpyCH4III ACNGT 1 cut(s) 686
HpyCH4IV ACGT 2 cut(s) 527, 678
HpyF10VI GCNNNNNNNGC 8 cut(s) 72, 297, 407, 473, 626, 728, 758, 863
HpyF3I CTNAG 7 cut(s) 44, 62, 390, 753, 821, 897, 1010
HpySE526I ACGT 2 cut(s) 527, 678
Hsp92I GRCGYC 4 cut(s) 399, 416, 527, 861
Hsp92II CATG 2 cut(s) 259, 313
HspAI GCGC 3 cut(s) 267, 844, 861
KasI GGCGCC 1 cut(s) 860
Kpn2I TCCGGA 1 cut(s) 89
KroI GCCGGC 1 cut(s) 857
KroNI GCCGGC 1 cut(s) 859
KspI CCGCGG 1 cut(s) 292
LmnI GCTCC 3 cut(s) 115, 257, 318
LweI GCATC 3 cut(s) 75, 522, 793
MaeI CTAG 1 cut(s) 303
MaeII ACGT 2 cut(s) 527, 678
MaeIII GTNAC 1 cut(s) 1013
MbiI CCGCTC 1 cut(s) 933
MboII GAAGA 7 cut(s) 133, 361, 364, 388, 457, 973, 1012
MflI RGATCY 2 cut(s) 227, 838
MhlI GDGCHC 3 cut(s) 60, 120, 254
MluCI AATT 2 cut(s) 17, 925
MluI ACGCGT 1 cut(s) 186
Mly113I GGCGCC 1 cut(s) 861
MlyI GAGTC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 608, 638
MreI CGCCGGCG 1 cut(s) 857
MroI TCCGGA 1 cut(s) 89
MroNI GCCGGC 1 cut(s) 857
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 2 cut(s) 321, 576
MslI CAYNNNNRTG 2 cut(s) 260, 506
MspA1I CMGCKG 2 cut(s) 291, 790
MspR9I CCNGG 8 cut(s) 23, 71, 151, 220, 505, 548, 650, 744
Mva1269I GAATGC 1 cut(s) 892
MvaI CCWGG 2 cut(s) 71, 650
MvnI CGCG 4 cut(s) 170, 188, 291, 866
MwoI GCNNNNNNNGC 8 cut(s) 72, 297, 407, 473, 626, 728, 758, 863
NaeI GCCGGC 1 cut(s) 859
NarI GGCGCC 1 cut(s) 861
NciI CCSGG 6 cut(s) 23, 151, 220, 505, 548, 744
NgoMIV GCCGGC 1 cut(s) 857
NlaIII CATG 2 cut(s) 259, 313
NlaIV GGNNCC 6 cut(s) 57, 194, 229, 545, 582, 862
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 1013
NspV TTCGAA 3 cut(s) 144, 695, 884
PcsI WCGNNNNNNNCGW 2 cut(s) 245, 378
PctI GAATGC 1 cut(s) 892
PdiI GCCGGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 93, 146, 610, 692
PflFI GACNNNGTC 1 cut(s) 358
PfoI TCCNGGA 2 cut(s) 21, 742
PkrI GCNGC 6 cut(s) 409, 478, 520, 718, 721, 865
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 864
PmaCI CACGTG 1 cut(s) 679
PmlI CACGTG 1 cut(s) 679
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 679
Psp124BI GAGCTC 2 cut(s) 120, 254
Psp6I CCWGG 2 cut(s) 69, 648
PspCI CACGTG 1 cut(s) 679
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 2 cut(s) 69, 648
PspN4I GGNNCC 6 cut(s) 57, 194, 229, 545, 582, 862
PspPI GGNCC 6 cut(s) 28, 48, 73, 152, 549, 1006
PstNI CAGNNNCTG 1 cut(s) 1017
PsuI RGATCY 2 cut(s) 227, 838
PsyI GACNNNGTC 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 790
RseI CAYNNNNRTG 2 cut(s) 260, 506
SacI GAGCTC 2 cut(s) 120, 254
SacII CCGCGG 1 cut(s) 292
SaqAI TTAA 2 cut(s) 321, 576
SatI GCNGC 6 cut(s) 408, 477, 519, 717, 720, 864
Sau96I GGNCC 6 cut(s) 28, 48, 73, 152, 549, 1006
SchI GAGTC 1 cut(s) 116
ScrFI CCNGG 8 cut(s) 23, 71, 151, 220, 505, 548, 650, 744
SduI GDGCHC 3 cut(s) 60, 120, 254
SfaNI GCATC 3 cut(s) 75, 522, 793
SfoI GGCGCC 1 cut(s) 862
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 3 cut(s) 144, 695, 884
SgrAI CRCCGGYG 2 cut(s) 101, 857
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 260, 506
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 2 cut(s) 17, 925
SspDI GGCGCC 1 cut(s) 860
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 2 cut(s) 120, 254
StyD4I CCNGG 8 cut(s) 21, 69, 149, 218, 503, 546, 648, 742
StyI CCWWGG 2 cut(s) 51, 111
TaaI ACNGT 1 cut(s) 686
TaiI ACGT 2 cut(s) 530, 681
TaqII GACCGA 1 cut(s) 355
TasI AATT 2 cut(s) 17, 925
TauI GCSGC 6 cut(s) 410, 479, 521, 719, 722, 866
TfiI GAWTC 4 cut(s) 93, 146, 610, 692
Tru1I TTAA 2 cut(s) 321, 576
Tru9I TTAA 2 cut(s) 321, 576
TseFI GTSAC 1 cut(s) 1013
Tsp45I GTSAC 1 cut(s) 1013
TspDTI ATGAA 3 cut(s) 17, 298, 671
TspGWI ACGGA 3 cut(s) 602, 820, 821
Tth111I GACNNNGTC 1 cut(s) 358
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 2 cut(s) 17, 925
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.