MD14G1058800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013312
Physical Location & Seq
Forward (+)
6069013 .. 6070020
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1058800.v1.1.491

Sequence Viewer

Length: 1008 bp
ATGAAAGCGTCGGTCAAATTCCGTGAGGACCAGAAGCCATTGATGCGAGCCAAGGTCCCACTCAGCATCTTGGGCCTCCCCTTCCAGTCCGGCATCGTCGCCGGCGAGTCCAAGGAGCTCACTCTCAACCTCGGCACCTTCTTCGAATCCGGGCCGTCCATCAAGATCGCGTATCGTCCCAACGACGCGTGGAACCCTTTCTCCCTCGTCGTTAAGACCGGGACGGGATCCTTCGGATCGCCGATTTCTAGCTCCATGCTGATGAGCGCCGAGTTCAATCTCCTCGGCCGCGGGAGAAACCCTAGCTTCATGCTCCACTTCAAGCCGCAGTTCGGCGACTTCTCGATCAAGAAGTCGCAGTCATCGGTCTTCGAGAAGATCGTCGGTTCTCAAAACGACTCCGCTGTGGTGAAGACGCCCGTAGTGGAGGCGGAGTTTTCGGGAAAGAAAATCACGGTTTTGCCATCGGAGAATCCGGCGGATGGAGTTCTCACGGGTGTATTCTCCGGGATGGAGGTGGCGGCGAGGACGTCGATGCCGGTGAGGAACCGGGCTGTGGTGAGTTTGAGGTGGGGGGTTCGGGTTCCCGCGGAGGTGAAGAGATCTGGGGCGAATCCGACGGCGGGGATTGCTTTTCAGAAAATCCCATTCTTGGTGATGAACAAGATTGGGATCGAACACGTGGAAGGCGGGGATTCGAATGGCAAAACCACAAAGGCAGCGGCGGAGGATAAGGGGGTTACATTTCCGGGAAATGGTGAGGTGGCGGAGGCTTGTTTTAGGGTGAAGCATCAGCTGGAGGCTTTGCGGACGGAGAACGGGTTGCTGAGGAAAGCCGTGGAGGATCTGCGCCACGAAATCGCCGGCACCGCGAAGTCGATTGCTGAGAGTGAGATAAATGGGGGAAGTAGAAATTCGAGCGGGAAAGTGGATTGGCGGTCGAACGGGAATAAGAAGTCGCCGGAGGGCGATGTGGCGGAGGAACTGAAGAAGGCACTCGGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

336

Amino Acids

35.92

Weight (kDa)

9.59

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57990
fragaria_vesca FvH4_6g22460
malus_domestica MD12G1059200.v1.1 MD14G1058800.v1.1
prunus_persica Prupe.7G076000_v2.0.a1
pyrus_communis pycom12g05180 pycom14g04760
rosa_chinensis RchiOBHm_Chr3g0479871
rosa_laevigata RLG00000023529
rosa_multiflora Rmu_co8502759.1_g000001
rosa_roxburghii Rroxscaffold_6G00401940
rosa_rugosa Rorug03G0179100
rosa_samantha Rh3AG230000 Rh3BG262700 Rh3CG260000 Rh3DG256100
rosa_wichuraiana Rw0G015260 Rw0G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 533
AccB1I GGYRCC 2 cut(s) 134, 866
AccBSI CCGCTC 1 cut(s) 921
AccII CGCG 5 cut(s) 170, 188, 291, 590, 872
AclWI GGATC 5 cut(s) 222, 235, 244, 680, 852
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 2 cut(s) 17, 913
AcuI CTGAAG 1 cut(s) 1007
AcvI CACGTG 1 cut(s) 682
AcyI GRCGYC 2 cut(s) 416, 530
AfiI CCNNNNNNNGG 6 cut(s) 332, 482, 556, 623, 652, 755
AflIII ACRYGT 2 cut(s) 186, 679
AgsI TTSAA 2 cut(s) 277, 322
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 118, 252, 306, 796
AluI AGCT 4 cut(s) 118, 252, 306, 796
Alw21I GWGCWC 1 cut(s) 120
AlwI GGATC 5 cut(s) 222, 235, 244, 680, 852
AoxI GGCC 3 cut(s) 73, 152, 286
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 2 cut(s) 17, 913
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 2 cut(s) 269, 852
AspS9I GGNCC 4 cut(s) 28, 55, 73, 152
AsuC2I CCSGG 5 cut(s) 151, 220, 508, 551, 750
AsuHPI GGTGA 7 cut(s) 421, 553, 571, 607, 667, 770, 796
AsuII TTCGAA 2 cut(s) 144, 698
AvaII GGWCC 2 cut(s) 28, 55
BamHI GGATCC 1 cut(s) 227
BanI GGYRCC 2 cut(s) 134, 866
BanII GRGCYC 1 cut(s) 120
BbrPI CACGTG 1 cut(s) 682
BbsI GAAGAC 2 cut(s) 361, 419
Bbv12I GWGCWC 1 cut(s) 120
BbvCI CCTCAGC 1 cut(s) 827
BbvI GCAGC 1 cut(s) 731
BccI CCATC 4 cut(s) 167, 472, 476, 505
BceAI ACGGC 3 cut(s) 139, 636, 821
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BcnI CCSGG 5 cut(s) 151, 220, 508, 551, 750
BfaI CTAG 2 cut(s) 249, 303
BfoI RGCGCY 1 cut(s) 270
BglII AGATCT 1 cut(s) 602
BisI GCNGC 5 cut(s) 289, 326, 522, 720, 723
BlsI GCNGC 5 cut(s) 290, 327, 523, 721, 724
Bme1390I CCNGG 5 cut(s) 151, 220, 508, 551, 750
Bme18I GGWCC 2 cut(s) 28, 55
BmgT120I GGNCC 4 cut(s) 28, 55, 73, 152
BmiI GGNNCC 7 cut(s) 57, 136, 194, 229, 548, 585, 868
BmrFI CCNGG 5 cut(s) 151, 220, 508, 551, 750
BmsI GCATC 5 cut(s) 33, 75, 102, 525, 799
BpiI GAAGAC 2 cut(s) 361, 419
BpmI CTGGAG 1 cut(s) 818
Bpu10I CCTNAGC 1 cut(s) 827
Bpu14I TTCGAA 2 cut(s) 144, 698
BpuMI CCSGG 5 cut(s) 151, 220, 508, 551, 750
BsaAI YACGTR 1 cut(s) 682
BsaBI GATNNNNATC 1 cut(s) 671
BsaHI GRCGYC 2 cut(s) 416, 530
BsaJI CCNNGG 7 cut(s) 51, 111, 130, 283, 289, 588, 837
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 419, 449, 806, 836
Bsc4I CCNNNNNNNGG 6 cut(s) 332, 482, 556, 623, 652, 755
Bse118I RCCGGY 3 cut(s) 101, 538, 863
Bse1I ACTGG 1 cut(s) 85
Bse8I GATNNNNATC 1 cut(s) 671
BseDI CCNNGG 7 cut(s) 51, 111, 130, 283, 289, 588, 837
BseGI GGATG 2 cut(s) 487, 516
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 6 cut(s) 332, 482, 556, 623, 652, 755
BseMII CTCAG 3 cut(s) 76, 818, 876
BseNI ACTGG 1 cut(s) 85
BseRI GAGGAG 1 cut(s) 272
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 1 cut(s) 731
Bsh1236I CGCG 5 cut(s) 170, 188, 291, 590, 872
Bsh1285I CGRYCG 2 cut(s) 289, 941
BshFI GGCC 3 cut(s) 75, 154, 288
BshNI GGYRCC 2 cut(s) 134, 866
BsiEI CGRYCG 2 cut(s) 289, 941
BsiHKAI GWGCWC 1 cut(s) 120
BslFI GGGAC 3 cut(s) 41, 162, 235
BslI CCNNNNNNNGG 6 cut(s) 332, 482, 556, 623, 652, 755
BsmFI GGGAC 3 cut(s) 41, 162, 235
BsnI GGCC 3 cut(s) 75, 154, 288
Bsp119I TTCGAA 2 cut(s) 144, 698
Bsp1286I GDGCHC 1 cut(s) 120
Bsp143I GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
BspANI GGCC 3 cut(s) 75, 154, 288
BspCNI CTCAG 3 cut(s) 75, 819, 877
BspFNI CGCG 5 cut(s) 170, 188, 291, 590, 872
BspLI GGNNCC 7 cut(s) 57, 136, 194, 229, 548, 585, 868
BspPI GGATC 5 cut(s) 222, 235, 244, 680, 852
BspT104I TTCGAA 2 cut(s) 144, 698
BspT107I GGYRCC 2 cut(s) 134, 866
BsrBI CCGCTC 1 cut(s) 921
BsrFI RCCGGY 3 cut(s) 101, 538, 863
BsrI ACTGG 1 cut(s) 85
BssAI RCCGGY 3 cut(s) 101, 538, 863
BssECI CCNNGG 7 cut(s) 51, 111, 130, 283, 289, 588, 837
BssMI GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
BssNI GRCGYC 2 cut(s) 416, 530
BssT1I CCWWGG 2 cut(s) 51, 111
Bst4CI ACNGT 1 cut(s) 457
Bst6I CTCTTC 1 cut(s) 593
BstACI GRCGYC 2 cut(s) 416, 530
BstBAI YACGTR 1 cut(s) 682
BstBI TTCGAA 2 cut(s) 144, 698
BstC8I GCNNGC 3 cut(s) 48, 103, 865
BstDEI CTNAG 3 cut(s) 62, 827, 885
BstDSI CCRYGG 3 cut(s) 289, 588, 837
BstF5I GGATG 2 cut(s) 487, 516
BstFNI CGCG 5 cut(s) 170, 188, 291, 590, 872
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 2 cut(s) 269, 852
BstKTI GATC 8 cut(s) 168, 230, 239, 348, 381, 605, 675, 847
BstMBI GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
BstMCI CGRYCG 2 cut(s) 289, 941
BstMWI GCNNNNNNNGC 4 cut(s) 43, 72, 629, 869
BstSCI CCNGG 5 cut(s) 149, 218, 506, 549, 748
BstUI CGCG 5 cut(s) 170, 188, 291, 590, 872
BstV1I GCAGC 1 cut(s) 731
BstV2I GAAGAC 2 cut(s) 361, 419
BstX2I RGATCY 3 cut(s) 227, 602, 844
BstYI RGATCY 3 cut(s) 227, 602, 844
BstZI CGGCCG 1 cut(s) 286
BsuRI GGCC 3 cut(s) 75, 154, 288
BtgI CCRYGG 3 cut(s) 289, 588, 837
BtgZI GCGATG 1 cut(s) 984
BtsCI GGATG 2 cut(s) 487, 516
BtsIMutI CAGTG 1 cut(s) 1003
Cac8I GCNNGC 3 cut(s) 48, 103, 865
CfoI GCGC 2 cut(s) 269, 852
Cfr10I RCCGGY 3 cut(s) 101, 538, 863
Cfr13I GGNCC 4 cut(s) 28, 55, 73, 152
Cfr42I CCGCGG 2 cut(s) 292, 591
CseI GACGC 2 cut(s) 194, 424
CviAII CATG 2 cut(s) 256, 310
DdeI CTNAG 3 cut(s) 62, 827, 885
DpnI GATC 8 cut(s) 167, 229, 238, 347, 380, 604, 674, 846
DpnII GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
EaeI YGGCCR 1 cut(s) 286
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 593
EarI CTCTTC 1 cut(s) 593
EciI GGCGGA 5 cut(s) 446, 494, 740, 782, 992
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 1 cut(s) 286
Eco130I CCWWGG 2 cut(s) 51, 111
Eco24I GRGCYC 1 cut(s) 120
Eco47I GGWCC 2 cut(s) 28, 55
Eco52I CGGCCG 1 cut(s) 286
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 1007
Eco72I CACGTG 1 cut(s) 682
EcoICRI GAGCTC 1 cut(s) 118
EcoO109I RGGNCCY 1 cut(s) 55
EcoT14I CCWWGG 2 cut(s) 51, 111
EcoT38I GRGCYC 1 cut(s) 120
ErhI CCWWGG 2 cut(s) 51, 111
FaeI CATG 2 cut(s) 259, 313
FaiI YATR 2 cut(s) 257, 311
FaqI GGGAC 3 cut(s) 41, 162, 235
FatI CATG 2 cut(s) 255, 309
FauI CCCGC 5 cut(s) 284, 595, 616, 683, 914
Fnu4HI GCNGC 5 cut(s) 289, 326, 522, 720, 723
FokI GGATG 2 cut(s) 494, 523
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 5 cut(s) 289, 326, 522, 720, 723
FspBI CTAG 2 cut(s) 249, 303
GlaI GCGC 2 cut(s) 268, 851
GluI GCNGC 5 cut(s) 289, 326, 522, 720, 723
GsuI CTGGAG 1 cut(s) 818
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 3 cut(s) 75, 154, 288
HgaI GACGC 2 cut(s) 194, 424
HhaI GCGC 2 cut(s) 269, 852
Hin1I GRCGYC 2 cut(s) 416, 530
Hin1II CATG 2 cut(s) 259, 313
Hin6I GCGC 2 cut(s) 267, 850
HinP1I GCGC 2 cut(s) 267, 850
HinfI GANTC 6 cut(s) 107, 146, 398, 472, 613, 695
HphI GGTGA 7 cut(s) 421, 553, 571, 607, 667, 770, 796
Hpy188I TCNGA 4 cut(s) 236, 469, 618, 639
Hpy188III TCNNGA 5 cut(s) 163, 343, 349, 373, 441
Hpy99I CGWCG 7 cut(s) 13, 101, 188, 212, 386, 535, 622
HpyAV CCTTC 5 cut(s) 91, 148, 241, 680, 985
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 2 cut(s) 530, 681
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 72, 629, 869
HpyF3I CTNAG 3 cut(s) 62, 827, 885
HpySE526I ACGT 2 cut(s) 530, 681
Hsp92I GRCGYC 2 cut(s) 416, 530
Hsp92II CATG 2 cut(s) 259, 313
HspAI GCGC 2 cut(s) 267, 850
KroI GCCGGC 2 cut(s) 101, 863
KroNI GCCGGC 2 cut(s) 103, 865
KspI CCGCGG 2 cut(s) 292, 591
Kzo9I GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
LmnI GCTCC 3 cut(s) 115, 257, 318
Lsp1109I GCAGC 1 cut(s) 731
LweI GCATC 5 cut(s) 33, 75, 102, 525, 799
MaeI CTAG 2 cut(s) 249, 303
MaeII ACGT 2 cut(s) 530, 681
MaeIII GTNAC 2 cut(s) 739, 1001
MalI GATC 8 cut(s) 167, 229, 238, 347, 380, 604, 674, 846
MbiI CCGCTC 1 cut(s) 921
MboI GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
MboII GAAGA 6 cut(s) 133, 361, 388, 424, 610, 1000
MflI RGATCY 3 cut(s) 227, 602, 844
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 2 cut(s) 17, 913
MluI ACGCGT 1 cut(s) 186
MlyI GAGTC 2 cut(s) 116, 392
MmeI TCCRAC 1 cut(s) 641
MreI CGCCGGCG 1 cut(s) 101
MroNI GCCGGC 2 cut(s) 101, 863
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 5 cut(s) 291, 404, 590, 722, 796
MspR9I CCNGG 5 cut(s) 151, 220, 508, 551, 750
MvnI CGCG 5 cut(s) 170, 188, 291, 590, 872
MwoI GCNNNNNNNGC 4 cut(s) 43, 72, 629, 869
NaeI GCCGGC 2 cut(s) 103, 865
NciI CCSGG 5 cut(s) 151, 220, 508, 551, 750
NdeII GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
NgoMIV GCCGGC 2 cut(s) 101, 863
NlaIII CATG 2 cut(s) 259, 313
NlaIV GGNNCC 7 cut(s) 57, 136, 194, 229, 548, 585, 868
NmeAIII GCCGAG 3 cut(s) 111, 264, 295
NmuCI GTSAC 1 cut(s) 1001
NspV TTCGAA 2 cut(s) 144, 698
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 378
PdiI GCCGGC 2 cut(s) 103, 865
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 146, 472, 613, 695
PfoI TCCNGGA 2 cut(s) 506, 748
PkrI GCNGC 5 cut(s) 290, 327, 523, 721, 724
PleI GAGTC 2 cut(s) 115, 392
PmaCI CACGTG 1 cut(s) 682
PmlI CACGTG 1 cut(s) 682
PpsI GAGTC 2 cut(s) 115, 392
Ppu21I YACGTR 1 cut(s) 682
PpuMI RGGWCCY 1 cut(s) 55
Psp124BI GAGCTC 1 cut(s) 120
Psp5II RGGWCCY 1 cut(s) 55
PspCI CACGTG 1 cut(s) 682
PspN4I GGNNCC 7 cut(s) 57, 136, 194, 229, 548, 585, 868
PspPI GGNCC 4 cut(s) 28, 55, 73, 152
PspPPI RGGWCCY 1 cut(s) 55
PsuI RGATCY 3 cut(s) 227, 602, 844
PvuII CAGCTG 1 cut(s) 796
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 120
SacII CCGCGG 2 cut(s) 292, 591
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 5 cut(s) 289, 326, 522, 720, 723
Sau3AI GATC 8 cut(s) 165, 227, 236, 345, 378, 602, 672, 844
Sau96I GGNCC 4 cut(s) 28, 55, 73, 152
SchI GAGTC 2 cut(s) 116, 392
ScrFI CCNGG 5 cut(s) 151, 220, 508, 551, 750
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 5 cut(s) 33, 75, 102, 525, 799
Sfr303I CCGCGG 2 cut(s) 292, 591
SfuI TTCGAA 2 cut(s) 144, 698
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 2 cut(s) 292, 591
SinI GGWCC 2 cut(s) 28, 55
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 2 cut(s) 17, 913
SspMI CTAG 2 cut(s) 249, 303
SstI GAGCTC 1 cut(s) 120
StyD4I CCNGG 5 cut(s) 149, 218, 506, 549, 748
StyI CCWWGG 2 cut(s) 51, 111
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 2 cut(s) 533, 684
TaqI TCGA 9 cut(s) 144, 344, 372, 533, 675, 698, 878, 917, 941
TaqII GACCGA 2 cut(s) 355, 989
TasI AATT 2 cut(s) 17, 913
TauI GCSGC 4 cut(s) 291, 328, 524, 725
TfiI GAWTC 4 cut(s) 146, 472, 613, 695
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 1 cut(s) 719
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 3 cut(s) 17, 298, 674
TspGWI ACGGA 2 cut(s) 11, 827
VpaK11BI GGWCC 2 cut(s) 28, 55
XapI RAATTY 2 cut(s) 17, 913
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 2 cut(s) 249, 303
ZraI GACGTC 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.