pycom12g05180

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013312
Physical Location & Seq
Forward (+)
5045956 .. 5046978
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g05180.1

Sequence Viewer

Length: 1023 bp
ATGAAGGCGTCGGTCAAATTCCGGGAGGATCAGAAGCCGTTGCTGAGGGCCAAGGTGCCCCTCAGCATCCTGGGTCTGCCCTTCCAGTCCGGAATCGTCGCCGGTGAGTCCAAGGAGCTCACTCTCAACCTCGCCACCTTCTTCGTATCCGGGCCGTCCATCAAGATCGCGTATCGTCCCAACGACGCGTGGAACCCCTTCTCCCTCGTCATCAAGACCGGGACGGGATCCTTCGGATCGCCGATTTCGAGCTCCATGCTGATGAGCGCCGAGTTCAATCTCCTTGCCCGCGGGAGAAACCCTAGCTTCATGCTCCACTTCAAGCCCCAGTTCGGTGATTTTTCGATCAAGAAGACGCAGTCCTCGGTCTTCGAGAAGATCGTGGGTTCTGAGAAGGACGCCGTTGCGGCGGAGACGCCATTAGTCGAGGCGGAGTTTTCGGGGAAGAAAATTACGGTTTTGCCATCGGAGAGTCCGGCGGCTGGACTTCTTGCCGGTTTGTTCACCGGGATGGAGGTGGCGGCAAGGACGTCGATGCCGGTGAGGAACCGGGCCGTGGTGAACTTGAGGTGGGGGGTTAAGGTTCCGGCGGAGATAAAGAGTGCTGAGGTGAATCCGACGGCGGGAATTGCGTTTCAGAAAATCCCGTTCCTGGTGATGAACAAGATCGGGATCGAACACGTGGACGGTGGCGATTCGAAGGCCAAAACCACAAAGGCGGCGGCGGATAATGCGTTGACATTTCCGGGAAGTGCTGAGGTTGCGGAGGCTTGTTTTAGGGTGAAGCATCAGCTGGAGGCTCTGCGGACGGAGAACGGGTTGCTGAGAAAGGCCGTGGAGGATCTGCGCCACGAAATCGCCGGCGCCGCGAAGTCGATTTCGATTTCGAATGCGTTTCCTGAGAGTGAGATAAATGGGGGAAGTAGGAATTCGAGCGGGAAAGTGGATTGGCGTTCAAACGGGAAGAATAAGTCGCCGGAGGGTGATGTGGCGGAGGAACTGAAGAAGGCCCTCAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

341

Amino Acids

36.41

Weight (kDa)

9.5

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57990
fragaria_vesca FvH4_6g22460
malus_domestica MD12G1059200.v1.1 MD14G1058800.v1.1
prunus_persica Prupe.7G076000_v2.0.a1
pyrus_communis pycom12g05180 pycom14g04760
rosa_chinensis RchiOBHm_Chr3g0479871
rosa_laevigata RLG00000023529
rosa_multiflora Rmu_co8502759.1_g000001
rosa_roxburghii Rroxscaffold_6G00401940
rosa_rugosa Rorug03G0179100
rosa_samantha Rh3AG230000 Rh3BG262700 Rh3CG260000 Rh3DG256100
rosa_wichuraiana Rw0G015260 Rw0G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 533
AccB1I GGYRCC 2 cut(s) 55, 863
AccBSI CCGCTC 1 cut(s) 936
AccII CGCG 4 cut(s) 170, 188, 291, 869
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 6 cut(s) 36, 222, 235, 244, 680, 849
AcsI RAATTY 2 cut(s) 17, 928
AcuI CTGAAG 1 cut(s) 1022
AcvI CACGTG 1 cut(s) 682
AcyI GRCGYC 5 cut(s) 8, 399, 416, 530, 864
AfiI CCNNNNNNNGG 5 cut(s) 332, 482, 556, 623, 652
AflIII ACRYGT 2 cut(s) 186, 679
AgsI TTSAA 3 cut(s) 277, 322, 957
AjnI CCWGG 2 cut(s) 69, 651
AloI GAACNNNNNNTCC 2 cut(s) 185, 217
AluBI AGCT 4 cut(s) 118, 252, 306, 793
AluI AGCT 4 cut(s) 118, 252, 306, 793
Alw21I GWGCWC 2 cut(s) 120, 254
Alw26I GTCTC 1 cut(s) 407
AlwI GGATC 6 cut(s) 36, 222, 235, 244, 680, 849
AlwNI CAGNNNCTG 1 cut(s) 1020
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 6 cut(s) 48, 152, 552, 702, 831, 1008
ApoI RAATTY 2 cut(s) 17, 928
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 269, 849, 866
AspS9I GGNCC 4 cut(s) 48, 152, 552, 1009
AsuC2I CCSGG 6 cut(s) 23, 151, 220, 508, 551, 747
AsuHPI GGTGA 9 cut(s) 116, 347, 496, 553, 571, 622, 667, 793, 995
AsuII TTCGAA 2 cut(s) 698, 887
BaeGI GKGCMC 1 cut(s) 60
BamHI GGATCC 1 cut(s) 227
BanI GGYRCC 2 cut(s) 55, 863
BanII GRGCYC 2 cut(s) 120, 254
BbrPI CACGTG 1 cut(s) 682
BbsI GAAGAC 2 cut(s) 359, 361
Bbv12I GWGCWC 2 cut(s) 120, 254
BbvCI CCTCAGC 4 cut(s) 44, 62, 606, 756
BccI CCATC 3 cut(s) 167, 472, 505
BceAI ACGGC 6 cut(s) 22, 139, 386, 539, 636, 818
BcgI CGANNNNNNTGC 4 cut(s) 238, 272, 513, 547
BciT130I CCWGG 2 cut(s) 71, 653
BciVI GTATCC 1 cut(s) 157
BcnI CCSGG 6 cut(s) 23, 151, 220, 508, 551, 747
BcoDI GTCTC 1 cut(s) 407
BfaI CTAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 270, 867
BfuI GTATCC 1 cut(s) 157
BglI GCCNNNNNGGC 1 cut(s) 407
BisI GCNGC 6 cut(s) 408, 480, 522, 720, 723, 867
BlsI GCNGC 6 cut(s) 409, 481, 523, 721, 724, 868
Bme1390I CCNGG 8 cut(s) 23, 71, 151, 220, 508, 551, 653, 747
BmgT120I GGNCC 4 cut(s) 48, 152, 552, 1009
BmiI GGNNCC 6 cut(s) 57, 194, 229, 548, 585, 865
BmrFI CCNGG 8 cut(s) 23, 71, 151, 220, 508, 551, 653, 747
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 3 cut(s) 75, 525, 796
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 2 cut(s) 359, 361
BpmI CTGGAG 1 cut(s) 815
Bpu10I CCTNAGC 4 cut(s) 44, 62, 606, 756
Bpu14I TTCGAA 2 cut(s) 698, 887
BpuEI CTTGAG 1 cut(s) 586
BpuMI CCSGG 6 cut(s) 23, 151, 220, 508, 551, 747
BsaAI YACGTR 1 cut(s) 682
BsaBI GATNNNNATC 1 cut(s) 671
BsaHI GRCGYC 5 cut(s) 8, 399, 416, 530, 864
BsaJI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 555, 834
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 803, 833
Bsc4I CCNNNNNNNGG 5 cut(s) 332, 482, 556, 623, 652
Bse118I RCCGGY 4 cut(s) 101, 494, 538, 860
Bse1I ACTGG 2 cut(s) 85, 328
Bse8I GATNNNNATC 1 cut(s) 671
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 2 cut(s) 71, 653
BseDI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 555, 834
BseGI GGATG 2 cut(s) 66, 516
BseJI GATNNNNATC 1 cut(s) 671
BseLI CCNNNNNNNGG 5 cut(s) 332, 482, 556, 623, 652
BseMII CTCAG 7 cut(s) 35, 76, 381, 597, 747, 815, 891
BseNI ACTGG 2 cut(s) 85, 328
BseSI GKGCMC 1 cut(s) 60
Bsh1236I CGCG 4 cut(s) 170, 188, 291, 869
BshFI GGCC 6 cut(s) 50, 154, 554, 704, 833, 1010
BshNI GGYRCC 2 cut(s) 55, 863
BsiHKAI GWGCWC 2 cut(s) 120, 254
BslFI GGGAC 2 cut(s) 162, 235
BslI CCNNNNNNNGG 5 cut(s) 332, 482, 556, 623, 652
BsmAI GTCTC 1 cut(s) 407
BsmBI CGTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 162, 235
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 6 cut(s) 50, 154, 554, 704, 833, 1010
Bsp119I TTCGAA 2 cut(s) 698, 887
Bsp1286I GDGCHC 3 cut(s) 60, 120, 254
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
BspANI GGCC 6 cut(s) 50, 154, 554, 704, 833, 1010
BspCNI CTCAG 7 cut(s) 36, 75, 382, 598, 748, 816, 892
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 4 cut(s) 170, 188, 291, 869
BspLI GGNNCC 6 cut(s) 57, 194, 229, 548, 585, 865
BspPI GGATC 6 cut(s) 36, 222, 235, 244, 680, 849
BspT104I TTCGAA 2 cut(s) 698, 887
BspT107I GGYRCC 2 cut(s) 55, 863
BsrBI CCGCTC 1 cut(s) 936
BsrFI RCCGGY 4 cut(s) 101, 494, 538, 860
BsrI ACTGG 2 cut(s) 85, 328
BssAI RCCGGY 4 cut(s) 101, 494, 538, 860
BssECI CCNNGG 7 cut(s) 51, 70, 111, 289, 363, 555, 834
BssMI GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
BssNI GRCGYC 5 cut(s) 8, 399, 416, 530, 864
BssT1I CCWWGG 2 cut(s) 51, 111
Bst2UI CCWGG 2 cut(s) 71, 653
Bst4CI ACNGT 2 cut(s) 457, 689
BstACI GRCGYC 5 cut(s) 8, 399, 416, 530, 864
BstBAI YACGTR 1 cut(s) 682
BstBI TTCGAA 2 cut(s) 698, 887
BstC8I GCNNGC 2 cut(s) 289, 862
BstDEI CTNAG 8 cut(s) 44, 62, 390, 606, 756, 824, 900, 1013
BstDSI CCRYGG 3 cut(s) 289, 555, 834
BstF5I GGATG 2 cut(s) 66, 516
BstFNI CGCG 4 cut(s) 170, 188, 291, 869
BstH2I RGCGCY 2 cut(s) 270, 867
BstHHI GCGC 3 cut(s) 269, 849, 866
BstKTI GATC 9 cut(s) 31, 168, 230, 239, 348, 381, 669, 675, 844
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
BstMWI GCNNNNNNNGC 5 cut(s) 407, 629, 731, 761, 866
BstNI CCWGG 2 cut(s) 71, 653
BstSCI CCNGG 8 cut(s) 21, 69, 149, 218, 506, 549, 651, 745
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 4 cut(s) 170, 188, 291, 869
BstV2I GAAGAC 2 cut(s) 359, 361
BstX2I RGATCY 2 cut(s) 227, 841
BstYI RGATCY 2 cut(s) 227, 841
BsuI GTATCC 1 cut(s) 157
BsuRI GGCC 6 cut(s) 50, 154, 554, 704, 833, 1010
BtgI CCRYGG 3 cut(s) 289, 555, 834
BtsCI GGATG 2 cut(s) 66, 516
BtsIMutI CAGTG 1 cut(s) 1018
Cac8I GCNNGC 2 cut(s) 289, 862
CaiI CAGNNNCTG 1 cut(s) 1020
CfoI GCGC 3 cut(s) 269, 849, 866
Cfr10I RCCGGY 4 cut(s) 101, 494, 538, 860
Cfr13I GGNCC 4 cut(s) 48, 152, 552, 1009
Cfr42I CCGCGG 1 cut(s) 292
CseI GACGC 4 cut(s) 194, 364, 407, 424
CviAII CATG 2 cut(s) 256, 310
DdeI CTNAG 8 cut(s) 44, 62, 390, 606, 756, 824, 900, 1013
DinI GGCGCC 1 cut(s) 865
DpnI GATC 9 cut(s) 30, 167, 229, 238, 347, 380, 668, 674, 843
DpnII GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
EciI GGCGGA 5 cut(s) 425, 446, 605, 740, 1007
Ecl136II GAGCTC 2 cut(s) 118, 252
Eco130I CCWWGG 2 cut(s) 51, 111
Eco24I GRGCYC 2 cut(s) 120, 254
Eco53kI GAGCTC 2 cut(s) 118, 252
Eco57I CTGAAG 1 cut(s) 1022
Eco72I CACGTG 1 cut(s) 682
EcoICRI GAGCTC 2 cut(s) 118, 252
EcoO109I RGGNCCY 1 cut(s) 1009
EcoRI GAATTC 1 cut(s) 928
EcoRII CCWGG 2 cut(s) 69, 651
EcoT14I CCWWGG 2 cut(s) 51, 111
EcoT38I GRGCYC 2 cut(s) 120, 254
EgeI GGCGCC 1 cut(s) 865
EheI GGCGCC 1 cut(s) 865
ErhI CCWWGG 2 cut(s) 51, 111
Esp3I CGTCTC 1 cut(s) 407
FaeI CATG 2 cut(s) 259, 313
FaiI YATR 2 cut(s) 257, 311
FaqI GGGAC 2 cut(s) 162, 235
FatI CATG 2 cut(s) 255, 309
FauI CCCGC 4 cut(s) 284, 296, 616, 929
Fnu4HI GCNGC 6 cut(s) 408, 480, 522, 720, 723, 867
FokI GGATG 2 cut(s) 53, 523
FriOI GRGCYC 2 cut(s) 120, 254
Fsp4HI GCNGC 6 cut(s) 408, 480, 522, 720, 723, 867
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 268, 848, 865
GluI GCNGC 6 cut(s) 408, 480, 522, 720, 723, 867
GsuI CTGGAG 1 cut(s) 815
HaeII RGCGCY 2 cut(s) 270, 867
HaeIII GGCC 6 cut(s) 50, 154, 554, 704, 833, 1010
HgaI GACGC 4 cut(s) 194, 364, 407, 424
HhaI GCGC 3 cut(s) 269, 849, 866
Hin1I GRCGYC 5 cut(s) 8, 399, 416, 530, 864
Hin1II CATG 2 cut(s) 259, 313
Hin6I GCGC 3 cut(s) 267, 847, 864
HinP1I GCGC 3 cut(s) 267, 847, 864
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HinfI GANTC 5 cut(s) 93, 107, 472, 613, 695
HphI GGTGA 9 cut(s) 116, 347, 496, 553, 571, 622, 667, 793, 995
Hpy166II GTNNAC 4 cut(s) 504, 562, 685, 738
Hpy188I TCNGA 6 cut(s) 33, 236, 391, 469, 618, 639
Hpy188III TCNNGA 7 cut(s) 90, 163, 214, 349, 373, 670, 899
Hpy8I GTNNAC 4 cut(s) 504, 562, 685, 738
Hpy99I CGWCG 5 cut(s) 13, 101, 188, 535, 622
HpyAV CCTTC 7 cut(s) 91, 148, 208, 241, 388, 694, 1000
HpyCH4III ACNGT 2 cut(s) 457, 689
HpyCH4IV ACGT 2 cut(s) 530, 681
HpyF10VI GCNNNNNNNGC 5 cut(s) 407, 629, 731, 761, 866
HpyF3I CTNAG 8 cut(s) 44, 62, 390, 606, 756, 824, 900, 1013
HpySE526I ACGT 2 cut(s) 530, 681
Hsp92I GRCGYC 5 cut(s) 8, 399, 416, 530, 864
Hsp92II CATG 2 cut(s) 259, 313
HspAI GCGC 3 cut(s) 267, 847, 864
KasI GGCGCC 1 cut(s) 863
Kpn2I TCCGGA 1 cut(s) 89
KroI GCCGGC 1 cut(s) 860
KroNI GCCGGC 1 cut(s) 862
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
LmnI GCTCC 3 cut(s) 115, 257, 318
LweI GCATC 3 cut(s) 75, 525, 796
MaeI CTAG 1 cut(s) 303
MaeII ACGT 2 cut(s) 530, 681
MaeIII GTNAC 1 cut(s) 1016
MalI GATC 9 cut(s) 30, 167, 229, 238, 347, 380, 668, 674, 843
MbiI CCGCTC 1 cut(s) 936
MboI GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
MboII GAAGA 7 cut(s) 133, 361, 364, 388, 457, 976, 1015
MflI RGATCY 2 cut(s) 227, 841
MhlI GDGCHC 3 cut(s) 60, 120, 254
MluCI AATT 4 cut(s) 17, 450, 627, 928
MluI ACGCGT 1 cut(s) 186
Mly113I GGCGCC 1 cut(s) 864
MlyI GAGTC 2 cut(s) 116, 481
MmeI TCCRAC 1 cut(s) 641
MreI CGCCGGCG 1 cut(s) 860
MroI TCCGGA 1 cut(s) 89
MroNI GCCGGC 1 cut(s) 860
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 579
MslI CAYNNNNRTG 2 cut(s) 260, 509
MspA1I CMGCKG 2 cut(s) 291, 793
MspR9I CCNGG 8 cut(s) 23, 71, 151, 220, 508, 551, 653, 747
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 2 cut(s) 71, 653
MvnI CGCG 4 cut(s) 170, 188, 291, 869
MwoI GCNNNNNNNGC 5 cut(s) 407, 629, 731, 761, 866
NaeI GCCGGC 1 cut(s) 862
NarI GGCGCC 1 cut(s) 864
NciI CCSGG 6 cut(s) 23, 151, 220, 508, 551, 747
NdeII GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
NgoMIV GCCGGC 1 cut(s) 860
NlaIII CATG 2 cut(s) 259, 313
NlaIV GGNNCC 6 cut(s) 57, 194, 229, 548, 585, 865
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 1 cut(s) 1016
NspV TTCGAA 2 cut(s) 698, 887
PcsI WCGNNNNNNNCGW 2 cut(s) 245, 378
PctI GAATGC 1 cut(s) 895
PdiI GCCGGC 1 cut(s) 862
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 3 cut(s) 93, 613, 695
PflFI GACNNNGTC 1 cut(s) 358
PfoI TCCNGGA 2 cut(s) 21, 745
PkrI GCNGC 6 cut(s) 409, 481, 523, 721, 724, 868
PleI GAGTC 2 cut(s) 115, 480
PluTI GGCGCC 1 cut(s) 867
PmaCI CACGTG 1 cut(s) 682
PmlI CACGTG 1 cut(s) 682
PpsI GAGTC 2 cut(s) 115, 480
Ppu21I YACGTR 1 cut(s) 682
Psp124BI GAGCTC 2 cut(s) 120, 254
Psp6I CCWGG 2 cut(s) 69, 651
PspCI CACGTG 1 cut(s) 682
PspGI CCWGG 2 cut(s) 69, 651
PspN4I GGNNCC 6 cut(s) 57, 194, 229, 548, 585, 865
PspPI GGNCC 4 cut(s) 48, 152, 552, 1009
PstNI CAGNNNCTG 1 cut(s) 1020
PsuI RGATCY 2 cut(s) 227, 841
PsyI GACNNNGTC 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 793
RseI CAYNNNNRTG 2 cut(s) 260, 509
SacI GAGCTC 2 cut(s) 120, 254
SacII CCGCGG 1 cut(s) 292
SaqAI TTAA 1 cut(s) 579
SatI GCNGC 6 cut(s) 408, 480, 522, 720, 723, 867
Sau3AI GATC 9 cut(s) 28, 165, 227, 236, 345, 378, 666, 672, 841
Sau96I GGNCC 4 cut(s) 48, 152, 552, 1009
SchI GAGTC 2 cut(s) 116, 481
ScrFI CCNGG 8 cut(s) 23, 71, 151, 220, 508, 551, 653, 747
SduI GDGCHC 3 cut(s) 60, 120, 254
SfaNI GCATC 3 cut(s) 75, 525, 796
SfoI GGCGCC 1 cut(s) 865
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 2 cut(s) 698, 887
SgrAI CRCCGGYG 2 cut(s) 101, 860
SgrBI CCGCGG 1 cut(s) 292
SmiMI CAYNNNNRTG 2 cut(s) 260, 509
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 4 cut(s) 17, 450, 627, 928
SspDI GGCGCC 1 cut(s) 863
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 2 cut(s) 120, 254
StyD4I CCNGG 8 cut(s) 21, 69, 149, 218, 506, 549, 651, 745
StyI CCWWGG 2 cut(s) 51, 111
TaaI ACNGT 2 cut(s) 457, 689
TaiI ACGT 2 cut(s) 533, 684
TaqII GACCGA 1 cut(s) 355
TasI AATT 4 cut(s) 17, 450, 627, 928
TauI GCSGC 6 cut(s) 410, 482, 524, 722, 725, 869
TfiI GAWTC 3 cut(s) 93, 613, 695
Tru1I TTAA 1 cut(s) 579
Tru9I TTAA 1 cut(s) 579
TseFI GTSAC 1 cut(s) 1016
Tsp45I GTSAC 1 cut(s) 1016
TspDTI ATGAA 3 cut(s) 17, 298, 674
TspGWI ACGGA 1 cut(s) 824
Tth111I GACNNNGTC 1 cut(s) 358
XapI RAATTY 2 cut(s) 17, 928
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.