RLG00000023529

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013312
Physical Location & Seq
Reverse (-)
24950809 .. 24951825
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023529

Sequence Viewer

Length: 1017 bp
ATGAAAGCTTCAGTCAAATTCCGGGAGGACCAGAAGCCATTGCTGCGGGCCAAAGTGCCCCTCAGCATCTTGGGCTTGCCGTTCCAATCCGGAATCGTCGCCGGCGAATCCAAGGAGCTCACTCTCAACCTCGGCACCTTCTTCGAATCCGGGCCGTCGGTTCGGGTCGCCTACCGCCCCAACGACACGTGGAACCCCTTCTCCGTCGTCGTCAAGACCGGCACCGGCTCCTTCGGCTCTCCGTTCAACAGCTCCATGCTGATGAGCGCCGAATTCAATCTGCTCGGCCGCGGAAGCAACCCTAGCTTCATGCTCCACTTCAAGCCTCAGTTTGGGGACTTCTCCGTCAAGAAGTCGTCGCAGTCTTCGGCCCTGGATAAGATGCTGGGCTCGCAAGACGGCGTCGTTTCCGGGGAGAATCCGTCGATTAAGGTCGTGGAGGCGCCGGCCGTGGACGGATTGTTTCCCGGGAAGAAGATTACGGTTTTGCCCTTCGAGAAAGCTGCCTCCGGTGCCGTGACCGGTCTGTTCTCCGGGATGGAGGTGTCGGCGAGGACGTCGATGCCGGTGAGGAACCGGGCCGTGGTGAGCTTGCGGTGGGGAGTGAGGGTTCCGGCGGAGCTGAAGAGCGGGGATTATCGGACGGCGGGGATTGCGTTTCAGAAAATCCCGTTCCTGGTGATGAACAAGATCGGGATCGAGCACGTGGACGGTGGGGATTCGAAGGGGAGCAGCAACACAAATGCCGGTGCGGCATTGACGTTTCCGGGAAATGTGGAGGTGGCAGAGGCTTGTTTCACTGTGAAGCGTCAGTTGGAGGCTTTACAGAGCGAGAATGGGTTGCTGAGAAAAGCTGTGGAGGATCTGCGGCAAGAAATCGCCGGAGGGAGTTTCAATTCGGGGAAGTACCGTGGAATGGAGAGGAGTAGTGCGAGTTGGGGCGCCAGCAACAATGAGAAGAAATCGCAGGAGGGGGATGTGAGTGAGGAGTTGAAGAAGGCACTGATGGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

339

Amino Acids

36.07

Weight (kDa)

9.46

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57990
fragaria_vesca FvH4_6g22460
malus_domestica MD12G1059200.v1.1 MD14G1058800.v1.1
prunus_persica Prupe.7G076000_v2.0.a1
pyrus_communis pycom12g05180 pycom14g04760
rosa_chinensis RchiOBHm_Chr3g0479871
rosa_laevigata RLG00000023529
rosa_multiflora Rmu_co8502759.1_g000001
rosa_roxburghii Rroxscaffold_6G00401940
rosa_rugosa Rorug03G0179100
rosa_samantha Rh3AG230000 Rh3BG262700 Rh3CG260000 Rh3DG256100
rosa_wichuraiana Rw0G015260 Rw0G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 560
AccB1I GGYRCC 5 cut(s) 134, 221, 442, 512, 941
AccBSI CCGCTC 1 cut(s) 630
AccII CGCG 1 cut(s) 291
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 2 cut(s) 704, 870
AcoI YGGCCR 2 cut(s) 286, 447
AcsI RAATTY 2 cut(s) 17, 272
AcuI CTGAAG 1 cut(s) 644
AcvI CACGTG 2 cut(s) 189, 706
AcyI GRCGYC 4 cut(s) 402, 443, 557, 942
AfaI GTAC 1 cut(s) 908
AfiI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 916
AflIII ACRYGT 1 cut(s) 186
AgeI ACCGGT 1 cut(s) 521
AgsI TTSAA 5 cut(s) 247, 277, 322, 895, 994
AjnI CCWGG 2 cut(s) 372, 675
AjuI GAANNNNNNNTTGG 2 cut(s) 797, 829
AloI GAACNNNNNNTCC 4 cut(s) 185, 217, 594, 626
AluBI AGCT 8 cut(s) 8, 118, 252, 306, 503, 591, 622, 854
AluI AGCT 8 cut(s) 8, 118, 252, 306, 503, 591, 622, 854
Alw21I GWGCWC 2 cut(s) 120, 705
AlwI GGATC 2 cut(s) 704, 870
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 6 cut(s) 48, 152, 286, 369, 447, 579
ApeKI GCWGC 3 cut(s) 43, 503, 732
ApoI RAATTY 2 cut(s) 17, 272
AsiGI ACCGGT 1 cut(s) 521
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 269, 445, 944
AspS9I GGNCC 5 cut(s) 28, 48, 152, 370, 579
AsuC2I CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 768
AsuHPI GGTGA 3 cut(s) 580, 598, 691
AsuII TTCGAA 2 cut(s) 144, 722
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 5 cut(s) 134, 221, 442, 512, 941
BanII GRGCYC 2 cut(s) 120, 392
BbrPI CACGTG 2 cut(s) 189, 706
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 2 cut(s) 120, 705
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 3 cut(s) 30, 490, 744
BccI CCATC 2 cut(s) 532, 1000
BceAI ACGGC 7 cut(s) 64, 139, 415, 434, 500, 566, 660
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 485, 519
BciT130I CCWGG 2 cut(s) 374, 677
BcnI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 768
BfaI CTAG 1 cut(s) 303
BfoI RGCGCY 3 cut(s) 270, 446, 945
BglI GCCNNNNNGGC 1 cut(s) 752
BisI GCNGC 6 cut(s) 44, 289, 504, 733, 753, 869
BlsI GCNGC 6 cut(s) 45, 290, 505, 734, 754, 870
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 5 cut(s) 28, 48, 152, 370, 579
BmiI GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 943
BmsI GCATC 3 cut(s) 75, 372, 552
BpiI GAAGAC 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 2 cut(s) 144, 722
BpuMI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 768
BsaAI YACGTR 2 cut(s) 189, 706
BsaBI GATNNNNATC 1 cut(s) 695
BsaHI GRCGYC 4 cut(s) 402, 443, 557, 942
BsaJI CCNNGG 9 cut(s) 111, 130, 289, 372, 411, 450, 467, 582, 910
BsaWI WCCGGW 3 cut(s) 89, 509, 521
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 407, 437, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 583, 676, 916
Bse118I RCCGGY 7 cut(s) 101, 218, 224, 445, 521, 565, 746
Bse3DI GCAATG 1 cut(s) 38
Bse8I GATNNNNATC 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 2 cut(s) 374, 677
BseDI CCNNGG 9 cut(s) 111, 130, 289, 372, 411, 450, 467, 582, 910
BseGI GGATG 2 cut(s) 543, 982
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 916
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 76, 341, 836
BseRI GAGGAG 2 cut(s) 937, 1001
BseSI GKGCMC 1 cut(s) 60
BseX3I CGGCCG 2 cut(s) 286, 447
BseXI GCAGC 3 cut(s) 30, 490, 744
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 291
Bsh1285I CGRYCG 2 cut(s) 289, 450
BshFI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BshNI GGYRCC 5 cut(s) 134, 221, 442, 512, 941
BshTI ACCGGT 1 cut(s) 521
BsiEI CGRYCG 2 cut(s) 289, 450
BsiHKAI GWGCWC 2 cut(s) 120, 705
BsiHKCI CYCGRG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 916
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 2 cut(s) 144, 722
Bsp1286I GDGCHC 4 cut(s) 60, 120, 392, 705
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 3 cut(s) 690, 696, 862
BspANI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BspCNI CTCAG 3 cut(s) 75, 340, 837
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 943
BspPI GGATC 2 cut(s) 704, 870
BspQI GCTCTTC 1 cut(s) 620
BspT104I TTCGAA 2 cut(s) 144, 722
BspT107I GGYRCC 5 cut(s) 134, 221, 442, 512, 941
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 38
BsrFI RCCGGY 7 cut(s) 101, 218, 224, 445, 521, 565, 746
BssAI RCCGGY 7 cut(s) 101, 218, 224, 445, 521, 565, 746
BssECI CCNNGG 9 cut(s) 111, 130, 289, 372, 411, 450, 467, 582, 910
BssMI GATC 3 cut(s) 690, 696, 862
BssNI GRCGYC 4 cut(s) 402, 443, 557, 942
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 374, 677
Bst4CI ACNGT 4 cut(s) 484, 713, 802, 911
Bst6I CTCTTC 1 cut(s) 620
BstACI GRCGYC 4 cut(s) 402, 443, 557, 942
BstBAI YACGTR 2 cut(s) 189, 706
BstBI TTCGAA 2 cut(s) 144, 722
BstC8I GCNNGC 7 cut(s) 48, 77, 103, 392, 447, 593, 946
BstDEI CTNAG 3 cut(s) 62, 327, 845
BstDSI CCRYGG 4 cut(s) 289, 450, 582, 910
BstF5I GGATG 2 cut(s) 543, 982
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 3 cut(s) 270, 446, 945
BstHHI GCGC 3 cut(s) 269, 445, 944
BstKTI GATC 3 cut(s) 693, 699, 865
BstMBI GATC 3 cut(s) 690, 696, 862
BstMCI CGRYCG 2 cut(s) 289, 450
BstMWI GCNNNNNNNGC 9 cut(s) 43, 72, 234, 294, 303, 391, 512, 653, 752
BstNI CCWGG 2 cut(s) 374, 677
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 3 cut(s) 30, 490, 744
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 1 cut(s) 862
BstYI RGATCY 1 cut(s) 862
BstZI CGGCCG 2 cut(s) 286, 447
BsuRI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BtgI CCRYGG 4 cut(s) 289, 450, 582, 910
BtsCI GGATG 2 cut(s) 543, 982
BtsIMutI CAGTG 2 cut(s) 798, 1001
Cac8I GCNNGC 7 cut(s) 48, 77, 103, 392, 447, 593, 946
CfoI GCGC 3 cut(s) 269, 445, 944
Cfr10I RCCGGY 7 cut(s) 101, 218, 224, 445, 521, 565, 746
Cfr13I GGNCC 5 cut(s) 28, 48, 152, 370, 579
Cfr42I CCGCGG 1 cut(s) 292
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 2 cut(s) 391, 797
Csp6I GTAC 1 cut(s) 907
CspAI ACCGGT 1 cut(s) 521
CviAII CATG 2 cut(s) 256, 310
CviQI GTAC 1 cut(s) 907
DdeI CTNAG 3 cut(s) 62, 327, 845
DinI GGCGCC 2 cut(s) 444, 943
DpnI GATC 3 cut(s) 692, 698, 864
DpnII GATC 3 cut(s) 690, 696, 862
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 2 cut(s) 286, 447
EagI CGGCCG 2 cut(s) 286, 447
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 2 cut(s) 286, 447
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 2 cut(s) 120, 392
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 2 cut(s) 286, 447
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 644
Eco72I CACGTG 2 cut(s) 189, 706
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 372, 675
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 2 cut(s) 120, 392
EgeI GGCGCC 2 cut(s) 444, 943
EheI GGCGCC 2 cut(s) 444, 943
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 2 cut(s) 259, 313
FaiI YATR 2 cut(s) 257, 311
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 255, 309
FauI CCCGC 3 cut(s) 39, 623, 640
Fnu4HI GCNGC 6 cut(s) 44, 289, 504, 733, 753, 869
FokI GGATG 2 cut(s) 550, 989
FriOI GRGCYC 2 cut(s) 120, 392
Fsp4HI GCNGC 6 cut(s) 44, 289, 504, 733, 753, 869
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 268, 444, 943
GluI GCNGC 6 cut(s) 44, 289, 504, 733, 753, 869
GsaI CCCAGC 1 cut(s) 389
HaeII RGCGCY 3 cut(s) 270, 446, 945
HaeIII GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
HgaI GACGC 2 cut(s) 391, 797
HhaI GCGC 3 cut(s) 269, 445, 944
Hin1I GRCGYC 4 cut(s) 402, 443, 557, 942
Hin1II CATG 2 cut(s) 259, 313
Hin6I GCGC 3 cut(s) 267, 443, 942
HinP1I GCGC 3 cut(s) 267, 443, 942
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 5 cut(s) 93, 107, 146, 418, 719
HphI GGTGA 3 cut(s) 580, 598, 691
Hpy166II GTNNAC 2 cut(s) 454, 709
Hpy188I TCNGA 2 cut(s) 642, 663
Hpy188III TCNNGA 5 cut(s) 90, 214, 349, 496, 694
Hpy8I GTNNAC 2 cut(s) 454, 709
Hpy99I CGWCG 8 cut(s) 101, 160, 209, 212, 361, 407, 427, 562
HpyAV CCTTC 6 cut(s) 148, 208, 241, 502, 718, 991
HpyCH4III ACNGT 4 cut(s) 484, 713, 802, 911
HpyCH4IV ACGT 4 cut(s) 188, 557, 705, 761
HpyF10VI GCNNNNNNNGC 9 cut(s) 43, 72, 234, 294, 303, 391, 512, 653, 752
HpyF3I CTNAG 3 cut(s) 62, 327, 845
HpySE526I ACGT 4 cut(s) 188, 557, 705, 761
Hsp92I GRCGYC 4 cut(s) 402, 443, 557, 942
Hsp92II CATG 2 cut(s) 259, 313
HspAI GCGC 3 cut(s) 267, 443, 942
KasI GGCGCC 2 cut(s) 442, 941
Kpn2I TCCGGA 1 cut(s) 89
KroI GCCGGC 2 cut(s) 101, 445
KroNI GCCGGC 2 cut(s) 103, 447
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 3 cut(s) 690, 696, 862
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 6 cut(s) 115, 233, 257, 318, 619, 729
Lsp1109I GCAGC 3 cut(s) 30, 490, 744
LweI GCATC 3 cut(s) 75, 372, 552
MaeI CTAG 1 cut(s) 303
MaeII ACGT 4 cut(s) 188, 557, 705, 761
MaeIII GTNAC 1 cut(s) 517
MalI GATC 3 cut(s) 692, 698, 864
MbiI CCGCTC 1 cut(s) 630
MboI GATC 3 cut(s) 690, 696, 862
MboII GAAGA 7 cut(s) 133, 357, 484, 487, 637, 970, 1006
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 4 cut(s) 60, 120, 392, 705
MluCI AATT 3 cut(s) 17, 272, 895
Mly113I GGCGCC 2 cut(s) 443, 942
MmeI TCCRAC 1 cut(s) 795
MreI CGCCGGCG 1 cut(s) 101
MroI TCCGGA 1 cut(s) 89
MroNI GCCGGC 2 cut(s) 101, 445
MroXI GAANNNNTTC 1 cut(s) 197
MseI TTAA 1 cut(s) 429
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 291
MvaI CCWGG 2 cut(s) 374, 677
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 9 cut(s) 43, 72, 234, 294, 303, 391, 512, 653, 752
NaeI GCCGGC 2 cut(s) 103, 447
NarI GGCGCC 2 cut(s) 443, 942
NciI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 768
NdeII GATC 3 cut(s) 690, 696, 862
NgoMIV GCCGGC 2 cut(s) 101, 445
NlaIII CATG 2 cut(s) 259, 313
NlaIV GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 943
NmeAIII GCCGAG 2 cut(s) 111, 264
NmuCI GTSAC 1 cut(s) 517
NspV TTCGAA 2 cut(s) 144, 722
PciSI GCTCTTC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 554
PdiI GCCGGC 2 cut(s) 103, 447
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 5 cut(s) 93, 107, 146, 418, 719
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 3 cut(s) 21, 533, 766
PinAI ACCGGT 1 cut(s) 521
PkrI GCNGC 6 cut(s) 45, 290, 505, 734, 754, 870
PluTI GGCGCC 2 cut(s) 446, 945
PmaCI CACGTG 2 cut(s) 189, 706
PmlI CACGTG 2 cut(s) 189, 706
Ppu21I YACGTR 2 cut(s) 189, 706
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 372, 675
PspCI CACGTG 2 cut(s) 189, 706
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 2 cut(s) 372, 675
PspN4I GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 943
PspPI GGNCC 5 cut(s) 28, 48, 152, 370, 579
PsuI RGATCY 1 cut(s) 862
PsyI GACNNNGTC 1 cut(s) 401
RsaI GTAC 1 cut(s) 908
RsaNI GTAC 1 cut(s) 907
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 120
SacII CCGCGG 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 620
SaqAI TTAA 1 cut(s) 429
SatI GCNGC 6 cut(s) 44, 289, 504, 733, 753, 869
Sau3AI GATC 3 cut(s) 690, 696, 862
Sau96I GGNCC 5 cut(s) 28, 48, 152, 370, 579
SduI GDGCHC 4 cut(s) 60, 120, 392, 705
SfaNI GCATC 3 cut(s) 75, 372, 552
SfoI GGCGCC 2 cut(s) 444, 943
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 2 cut(s) 144, 722
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 28
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 3 cut(s) 17, 272, 895
SspDI GGCGCC 2 cut(s) 442, 941
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 120
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 4 cut(s) 484, 713, 802, 911
TaiI ACGT 4 cut(s) 191, 560, 708, 764
TaqI TCGA 6 cut(s) 144, 425, 495, 560, 699, 722
TasI AATT 3 cut(s) 17, 272, 895
TauI GCSGC 3 cut(s) 291, 755, 871
TfiI GAWTC 5 cut(s) 93, 107, 146, 418, 719
Tru1I TTAA 1 cut(s) 429
Tru9I TTAA 1 cut(s) 429
TscAI CASTG 2 cut(s) 805, 1008
TseFI GTSAC 1 cut(s) 517
TseI GCWGC 3 cut(s) 43, 503, 732
Tsp45I GTSAC 1 cut(s) 517
TspDTI ATGAA 3 cut(s) 17, 298, 698
TspGWI ACGGA 5 cut(s) 193, 231, 334, 411, 471
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 2 cut(s) 805, 1008
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 2 cut(s) 17, 272
XmaI CCCGGG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.