Rw0G019240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00864
OG0013312
Physical Location & Seq
Forward (+)
78534 .. 79556
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G019240.1

Sequence Viewer

Length: 1023 bp
ATGAAAGCTTCAGTCAAATTCCGGGAGGACCAGAAGCCATTGCTGCGGGCCAAAGTGCCCCTCAGCATCTTGGGCTTGCCGTTCCAATCCGGAATCGTCGCCGGCGAGTCCAAGGAGCTCACTCTCAACCTCGGCACCTTCTTCGAATCCGGGCCGTCGGTTCGGGTCGCCTACCGCCCCAACGACGCGTGGAACCCCTTCTCCGTCGTCGTCAAGACCGGCACCGGCTCCTTCGGCTCTCCGTTCAACAGCTCCATGCTGATGAGCGCCGAATTCAATCTGCTCGGCCGCGGAAGCAACCCTAGCTTCATGCTCCACTTCAAGCCTCAGTTTGGGGACTTCTCCGTCAAGAAGTCGTCGCAGTCCTCGGCCCTGGATAAGATGCTGGGCTCGCAAGACGGCGTCGTTTCCGGGGAGAATCCGTCGATCAAGGTCGTGGAGGCGCCGGCCGTGGACGGATTGTTTCCCGGGAAGAAGATTACGGTTTTGCCCTTCGAGAGAGCTGCCTCCGGTGCCGTCTCCGGTCTGTTCTCCGGGATGGAGGTGTCGGCGAGGACGTCGATGCCGGTGAGGAACCGGGCCGTGGTGAGCTTGCGCTGGGGAGTGAGGGTTCCGGCGGAGCTGAAGAGCGGGGATTATCGGACGGCGGGGATTGCGTTGCAGAAAATCCCGTTCCTGGTGATGAACAAGATCGGGATCGAGCACGTGGACGGTGGGGATTCGAAGGGAAGCAGCAACACAAATGCCGGTGCTGCTGCGGCGTTGACGTTTCCGGGAAATGTGGAGGTGGCGGAGGCTTGTTTCACTGTGAAGCGTCAGTTAGAGGCTTTACAGAGCGAGAATGGACTGCTGAGAAAAGCTGTGGAGGATCTGCGGCAAGAAATCGGCGGAGGGAGTTTCAATTCGGGGAAGTACCGTGGAGTGGAGAGGAGTAGTGGGAATTGGGGCTCCAGCAACAATGACAAGAAATCGCAAGAGGGAGATGTGAGTGAGGAGTTGAAGAAGGCTCTAATGGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

340

Amino Acids

36.13

Weight (kDa)

9.47

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013312)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57990
fragaria_vesca FvH4_6g22460
malus_domestica MD12G1059200.v1.1 MD14G1058800.v1.1
prunus_persica Prupe.7G076000_v2.0.a1
pyrus_communis pycom12g05180 pycom14g04760
rosa_chinensis RchiOBHm_Chr3g0479871
rosa_laevigata RLG00000023529
rosa_multiflora Rmu_co8502759.1_g000001
rosa_roxburghii Rroxscaffold_6G00401940
rosa_rugosa Rorug03G0179100
rosa_samantha Rh3AG230000 Rh3BG262700 Rh3CG260000 Rh3DG256100
rosa_wichuraiana Rw0G015260 Rw0G019240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 560
AccB1I GGYRCC 4 cut(s) 134, 221, 442, 512
AccBSI CCGCTC 1 cut(s) 630
AccII CGCG 2 cut(s) 188, 291
AccIII TCCGGA 1 cut(s) 89
AclWI GGATC 2 cut(s) 704, 876
AcoI YGGCCR 2 cut(s) 286, 447
AcsI RAATTY 2 cut(s) 17, 272
AcuI CTGAAG 1 cut(s) 644
AcvI CACGTG 1 cut(s) 706
AcyI GRCGYC 3 cut(s) 402, 443, 557
AfaI GTAC 1 cut(s) 914
AfiI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 922
AflIII ACRYGT 1 cut(s) 186
AgsI TTSAA 5 cut(s) 247, 277, 322, 901, 1000
AjnI CCWGG 2 cut(s) 372, 675
AloI GAACNNNNNNTCC 4 cut(s) 185, 217, 594, 626
AluBI AGCT 8 cut(s) 8, 118, 252, 306, 503, 591, 622, 860
AluI AGCT 8 cut(s) 8, 118, 252, 306, 503, 591, 622, 860
Alw21I GWGCWC 2 cut(s) 120, 705
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 2 cut(s) 704, 876
Ama87I CYCGRG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 89
AoxI GGCC 6 cut(s) 48, 152, 286, 369, 447, 579
ApeKI GCWGC 5 cut(s) 43, 503, 732, 752, 755
ApoI RAATTY 2 cut(s) 17, 272
Asp700I GAANNNNTTC 1 cut(s) 197
AspLEI GCGC 3 cut(s) 269, 445, 597
AspS9I GGNCC 5 cut(s) 28, 48, 152, 370, 579
AsuC2I CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 774
AsuHPI GGTGA 3 cut(s) 580, 598, 691
AsuII TTCGAA 2 cut(s) 144, 722
AvaI CYCGRG 1 cut(s) 467
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 4 cut(s) 134, 221, 442, 512
BanII GRGCYC 3 cut(s) 120, 392, 950
BbrPI CACGTG 1 cut(s) 706
Bbv12I GWGCWC 2 cut(s) 120, 705
BbvCI CCTCAGC 1 cut(s) 62
BbvI GCAGC 5 cut(s) 30, 490, 739, 742, 744
BccI CCATC 1 cut(s) 532
BceAI ACGGC 7 cut(s) 64, 139, 415, 434, 500, 566, 660
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 485, 519
BciT130I CCWGG 2 cut(s) 374, 677
BcnI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 774
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 1 cut(s) 303
BfoI RGCGCY 2 cut(s) 270, 446
BisI GCNGC 8 cut(s) 44, 289, 504, 733, 753, 756, 759, 875
BlsI GCNGC 8 cut(s) 45, 290, 505, 734, 754, 757, 760, 876
Bme18I GGWCC 1 cut(s) 28
BmeT110I CYCGRG 1 cut(s) 467
BmgT120I GGNCC 5 cut(s) 28, 48, 152, 370, 579
BmiI GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 949
BmsI GCATC 3 cut(s) 75, 372, 552
BpmI CTGGAG 1 cut(s) 934
Bpu10I CCTNAGC 1 cut(s) 62
Bpu14I TTCGAA 2 cut(s) 144, 722
BpuMI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 774
BsaAI YACGTR 1 cut(s) 706
BsaBI GATNNNNATC 1 cut(s) 695
BsaHI GRCGYC 3 cut(s) 402, 443, 557
BsaWI WCCGGW 3 cut(s) 89, 509, 521
BsaXI ACNNNNNCTCC 6 cut(s) 185, 215, 407, 437, 594, 624
Bsc4I CCNNNNNNNGG 4 cut(s) 332, 583, 676, 922
Bse118I RCCGGY 6 cut(s) 101, 218, 224, 445, 565, 746
Bse3DI GCAATG 1 cut(s) 38
Bse8I GATNNNNATC 1 cut(s) 695
BseAI TCCGGA 1 cut(s) 89
BseBI CCWGG 2 cut(s) 374, 677
BseGI GGATG 1 cut(s) 543
BseJI GATNNNNATC 1 cut(s) 695
BseLI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 922
BseMI GCAATG 1 cut(s) 38
BseMII CTCAG 3 cut(s) 76, 341, 842
BseRI GAGGAG 2 cut(s) 943, 1007
BseSI GKGCMC 1 cut(s) 60
BseX3I CGGCCG 2 cut(s) 286, 447
BseXI GCAGC 5 cut(s) 30, 490, 739, 742, 744
BseYI CCCAGC 2 cut(s) 385, 597
Bsh1236I CGCG 2 cut(s) 188, 291
Bsh1285I CGRYCG 2 cut(s) 289, 450
BshFI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BshNI GGYRCC 4 cut(s) 134, 221, 442, 512
BsiEI CGRYCG 2 cut(s) 289, 450
BsiHKAI GWGCWC 2 cut(s) 120, 705
BsiHKCI CYCGRG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 4 cut(s) 332, 583, 676, 922
BsmAI GTCTC 1 cut(s) 523
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BsoBI CYCGRG 1 cut(s) 467
Bsp119I TTCGAA 2 cut(s) 144, 722
Bsp1286I GDGCHC 5 cut(s) 60, 120, 392, 705, 950
Bsp13I TCCGGA 1 cut(s) 89
Bsp143I GATC 4 cut(s) 426, 690, 696, 868
BspANI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BspCNI CTCAG 3 cut(s) 75, 340, 843
BspEI TCCGGA 1 cut(s) 89
BspFNI CGCG 2 cut(s) 188, 291
BspLI GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 949
BspPI GGATC 2 cut(s) 704, 876
BspQI GCTCTTC 1 cut(s) 620
BspT104I TTCGAA 2 cut(s) 144, 722
BspT107I GGYRCC 4 cut(s) 134, 221, 442, 512
BsrBI CCGCTC 1 cut(s) 630
BsrDI GCAATG 1 cut(s) 38
BsrFI RCCGGY 6 cut(s) 101, 218, 224, 445, 565, 746
BssAI RCCGGY 6 cut(s) 101, 218, 224, 445, 565, 746
BssMI GATC 4 cut(s) 426, 690, 696, 868
BssNI GRCGYC 3 cut(s) 402, 443, 557
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 2 cut(s) 374, 677
Bst4CI ACNGT 4 cut(s) 484, 713, 808, 917
Bst6I CTCTTC 1 cut(s) 620
BstACI GRCGYC 3 cut(s) 402, 443, 557
BstBAI YACGTR 1 cut(s) 706
BstBI TTCGAA 2 cut(s) 144, 722
BstC8I GCNNGC 6 cut(s) 48, 77, 103, 392, 447, 593
BstDEI CTNAG 3 cut(s) 62, 327, 851
BstDSI CCRYGG 4 cut(s) 289, 450, 582, 916
BstF5I GGATG 1 cut(s) 543
BstFNI CGCG 2 cut(s) 188, 291
BstH2I RGCGCY 2 cut(s) 270, 446
BstHHI GCGC 3 cut(s) 269, 445, 597
BstKTI GATC 4 cut(s) 429, 693, 699, 871
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 4 cut(s) 426, 690, 696, 868
BstMCI CGRYCG 2 cut(s) 289, 450
BstNI CCWGG 2 cut(s) 374, 677
BstSLI GKGCMC 1 cut(s) 60
BstUI CGCG 2 cut(s) 188, 291
BstV1I GCAGC 5 cut(s) 30, 490, 739, 742, 744
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
BstZI CGGCCG 2 cut(s) 286, 447
BsuRI GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
BtgI CCRYGG 4 cut(s) 289, 450, 582, 916
BtsCI GGATG 1 cut(s) 543
BtsIMutI CAGTG 1 cut(s) 804
Cac8I GCNNGC 6 cut(s) 48, 77, 103, 392, 447, 593
CfoI GCGC 3 cut(s) 269, 445, 597
Cfr10I RCCGGY 6 cut(s) 101, 218, 224, 445, 565, 746
Cfr13I GGNCC 5 cut(s) 28, 48, 152, 370, 579
Cfr42I CCGCGG 1 cut(s) 292
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 3 cut(s) 194, 391, 803
Csp6I GTAC 1 cut(s) 913
CviAII CATG 2 cut(s) 256, 310
CviQI GTAC 1 cut(s) 913
DdeI CTNAG 3 cut(s) 62, 327, 851
DinI GGCGCC 1 cut(s) 444
DpnI GATC 4 cut(s) 428, 692, 698, 870
DpnII GATC 4 cut(s) 426, 690, 696, 868
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 2 cut(s) 286, 447
EagI CGGCCG 2 cut(s) 286, 447
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
EciI GGCGGA 3 cut(s) 632, 806, 903
Ecl136II GAGCTC 1 cut(s) 118
EclXI CGGCCG 2 cut(s) 286, 447
Eco130I CCWWGG 1 cut(s) 111
Eco24I GRGCYC 3 cut(s) 120, 392, 950
Eco47I GGWCC 1 cut(s) 28
Eco52I CGGCCG 2 cut(s) 286, 447
Eco53kI GAGCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 644
Eco72I CACGTG 1 cut(s) 706
Eco88I CYCGRG 1 cut(s) 467
EcoICRI GAGCTC 1 cut(s) 118
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 2 cut(s) 372, 675
EcoT14I CCWWGG 1 cut(s) 111
EcoT38I GRGCYC 3 cut(s) 120, 392, 950
EgeI GGCGCC 1 cut(s) 444
EheI GGCGCC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 111
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 2 cut(s) 259, 313
FaiI YATR 2 cut(s) 257, 311
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 255, 309
FauI CCCGC 3 cut(s) 39, 623, 640
Fnu4HI GCNGC 8 cut(s) 44, 289, 504, 733, 753, 756, 759, 875
FokI GGATG 1 cut(s) 550
FriOI GRGCYC 3 cut(s) 120, 392, 950
Fsp4HI GCNGC 8 cut(s) 44, 289, 504, 733, 753, 756, 759, 875
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 268, 444, 596
GluI GCNGC 8 cut(s) 44, 289, 504, 733, 753, 756, 759, 875
GsaI CCCAGC 2 cut(s) 389, 601
GsuI CTGGAG 1 cut(s) 934
HaeII RGCGCY 2 cut(s) 270, 446
HaeIII GGCC 6 cut(s) 50, 154, 288, 371, 449, 581
HgaI GACGC 3 cut(s) 194, 391, 803
HhaI GCGC 3 cut(s) 269, 445, 597
Hin1I GRCGYC 3 cut(s) 402, 443, 557
Hin1II CATG 2 cut(s) 259, 313
Hin6I GCGC 3 cut(s) 267, 443, 595
HinP1I GCGC 3 cut(s) 267, 443, 595
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 6
HinfI GANTC 5 cut(s) 93, 107, 146, 418, 719
HphI GGTGA 3 cut(s) 580, 598, 691
Hpy166II GTNNAC 3 cut(s) 454, 709, 765
Hpy188I TCNGA 1 cut(s) 642
Hpy188III TCNNGA 5 cut(s) 90, 214, 349, 496, 694
Hpy8I GTNNAC 3 cut(s) 454, 709, 765
Hpy99I CGWCG 9 cut(s) 101, 160, 188, 209, 212, 361, 407, 427, 562
HpyAV CCTTC 6 cut(s) 148, 208, 241, 502, 718, 997
HpyCH4III ACNGT 4 cut(s) 484, 713, 808, 917
HpyCH4IV ACGT 3 cut(s) 557, 705, 767
HpyCH4V TGCA 1 cut(s) 661
HpyF3I CTNAG 3 cut(s) 62, 327, 851
HpySE526I ACGT 3 cut(s) 557, 705, 767
Hsp92I GRCGYC 3 cut(s) 402, 443, 557
Hsp92II CATG 2 cut(s) 259, 313
HspAI GCGC 3 cut(s) 267, 443, 595
KasI GGCGCC 1 cut(s) 442
Kpn2I TCCGGA 1 cut(s) 89
KroI GCCGGC 2 cut(s) 101, 445
KroNI GCCGGC 2 cut(s) 103, 447
KspI CCGCGG 1 cut(s) 292
Kzo9I GATC 4 cut(s) 426, 690, 696, 868
LguI GCTCTTC 1 cut(s) 620
LmnI GCTCC 6 cut(s) 115, 233, 257, 318, 619, 953
Lsp1109I GCAGC 5 cut(s) 30, 490, 739, 742, 744
LweI GCATC 3 cut(s) 75, 372, 552
MaeI CTAG 1 cut(s) 303
MaeII ACGT 3 cut(s) 557, 705, 767
MalI GATC 4 cut(s) 428, 692, 698, 870
MbiI CCGCTC 1 cut(s) 630
MboI GATC 4 cut(s) 426, 690, 696, 868
MboII GAAGA 5 cut(s) 133, 484, 487, 637, 1012
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 5 cut(s) 60, 120, 392, 705, 950
MluCI AATT 4 cut(s) 17, 272, 901, 940
MluI ACGCGT 1 cut(s) 186
Mly113I GGCGCC 1 cut(s) 443
MlyI GAGTC 1 cut(s) 116
MreI CGCCGGCG 1 cut(s) 101
MroI TCCGGA 1 cut(s) 89
MroNI GCCGGC 2 cut(s) 101, 445
MroXI GAANNNNTTC 1 cut(s) 197
MslI CAYNNNNRTG 1 cut(s) 260
MspA1I CMGCKG 1 cut(s) 291
MvaI CCWGG 2 cut(s) 374, 677
MvnI CGCG 2 cut(s) 188, 291
NaeI GCCGGC 2 cut(s) 103, 447
NarI GGCGCC 1 cut(s) 443
NciI CCSGG 8 cut(s) 23, 151, 412, 468, 469, 535, 578, 774
NdeII GATC 4 cut(s) 426, 690, 696, 868
NgoMIV GCCGGC 2 cut(s) 101, 445
NlaIII CATG 2 cut(s) 259, 313
NlaIV GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 949
NmeAIII GCCGAG 3 cut(s) 111, 264, 347
NspV TTCGAA 2 cut(s) 144, 722
PciSI GCTCTTC 1 cut(s) 620
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 554
PdiI GCCGGC 2 cut(s) 103, 447
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 93, 146, 418, 719
PflFI GACNNNGTC 1 cut(s) 401
PfoI TCCNGGA 3 cut(s) 21, 533, 772
PkrI GCNGC 8 cut(s) 45, 290, 505, 734, 754, 757, 760, 876
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 446
PmaCI CACGTG 1 cut(s) 706
PmlI CACGTG 1 cut(s) 706
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 706
Psp124BI GAGCTC 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 372, 675
PspCI CACGTG 1 cut(s) 706
PspFI CCCAGC 2 cut(s) 385, 597
PspGI CCWGG 2 cut(s) 372, 675
PspN4I GGNNCC 9 cut(s) 136, 194, 223, 229, 444, 514, 575, 612, 949
PspPI GGNCC 5 cut(s) 28, 48, 152, 370, 579
PsuI RGATCY 1 cut(s) 868
PsyI GACNNNGTC 1 cut(s) 401
RsaI GTAC 1 cut(s) 914
RsaNI GTAC 1 cut(s) 913
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 120
SacII CCGCGG 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 620
SatI GCNGC 8 cut(s) 44, 289, 504, 733, 753, 756, 759, 875
Sau3AI GATC 4 cut(s) 426, 690, 696, 868
Sau96I GGNCC 5 cut(s) 28, 48, 152, 370, 579
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 5 cut(s) 60, 120, 392, 705, 950
SfaNI GCATC 3 cut(s) 75, 372, 552
SfoI GGCGCC 1 cut(s) 444
Sfr303I CCGCGG 1 cut(s) 292
SfuI TTCGAA 2 cut(s) 144, 722
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 1 cut(s) 292
SinI GGWCC 1 cut(s) 28
SmaI CCCGGG 1 cut(s) 469
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 4 cut(s) 17, 272, 901, 940
SspDI GGCGCC 1 cut(s) 442
SspMI CTAG 1 cut(s) 303
SstI GAGCTC 1 cut(s) 120
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 4 cut(s) 484, 713, 808, 917
TaiI ACGT 3 cut(s) 560, 708, 770
TaqI TCGA 6 cut(s) 144, 425, 495, 560, 699, 722
TasI AATT 4 cut(s) 17, 272, 901, 940
TauI GCSGC 3 cut(s) 291, 761, 877
TfiI GAWTC 4 cut(s) 93, 146, 418, 719
TscAI CASTG 1 cut(s) 811
TseI GCWGC 5 cut(s) 43, 503, 732, 752, 755
TspDTI ATGAA 3 cut(s) 17, 298, 698
TspGWI ACGGA 5 cut(s) 193, 231, 334, 411, 471
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 1 cut(s) 811
Tth111I GACNNNGTC 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 28
XapI RAATTY 2 cut(s) 17, 272
XmaI CCCGGG 1 cut(s) 467
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 1 cut(s) 303
ZraI GACGTC 1 cut(s) 558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.