MD12G1076900.v1.1

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0010029
Physical Location & Seq
Forward (+)
9377022 .. 9378361
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1076900.v1.1.491

Sequence Viewer

Length: 849 bp
ATGAAGGCTTCAAACAAGATCAGTACCGGGACCTTTAGCACGGTTTACAAGGCTGTTATGCCGTCTGGGCTAATCATATCTGTGAAGAGACTGAAATCTATGGACAGGACAATCATTCATCACCAGAATAAGATGATCCGAGAGCTCGAAAGGCTGAGCAAACTCTGTCATGATAACCTAGTGCGTCCTATCGGATTTGTTATTTACGAGGACGTTGCTCTCTTGCTGCATCATTACTTGCCCTATGGGACATTATGTCAGCTTCTTCATGAATCCACAAAGCTGCCCGAATATGAACCTGACTGGCCAAAAAGACTCTCTATTGCCATAGGAGTAGCAGAAGGGTTGGCTTTCCTTCACCATGTAGCGATTATCCATCTCGATATTTCTTCTGGTAATGTTCTTCTGGATGCTAACTCCAATCCTTTGGTTGGAGAGATTGAGATATCGAAGCTCTTGGATCCATCCAGAGGTACAGCCAGTATCAGCGCGGTTGCAGGCTCCTTTGGCTACATTCCTCCAGAATATGCATATACAATGCAAGTTACAGCGCCTGGAAATGTCTATAGCTATGGTGTTGTTTTGCTTGAGATCCTTACAACCAAATTACCAGTAGATGAAGCCTTTGACGAGGGGGTAGATTTGGTGACGTGGGTGCATAATGCTCCGGCAAGAGGCGAAACCCCGGAGCAGATTCTTGATGCAAGGCTCAGCACTGTGTCCTTCGGTTGGAGGAAAGAAATGCTTGCAGCACTGAAGATTGCTTTGCTCTGCACTGACAGCATACCTGCCAAACGTCCGAAAATGAAGAAGGTTGTGGAAATGCTGGAAGAAATCAAGAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

283

Amino Acids

31.28

Weight (kDa)

6.8

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 2 - 275 2.5e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 4 - 208 5.9e-27 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 796
AccII CGCG 1 cut(s) 491
AciI CCGC 1 cut(s) 491
AclWI GGATC 4 cut(s) 130, 455, 468, 586
AcoI YGGCCR 1 cut(s) 305
AcuI CTGAAG 1 cut(s) 776
AfaI GTAC 2 cut(s) 25, 475
AfiI CCNNNNNNNGG 4 cut(s) 431, 470, 674, 729
AgsI TTSAA 1 cut(s) 12
AhdI GACNNNNNGTC 1 cut(s) 255
AjiI CACGTC 1 cut(s) 651
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 5 cut(s) 145, 262, 283, 454, 570
AluI AGCT 5 cut(s) 145, 262, 283, 454, 570
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 4 cut(s) 130, 455, 468, 586
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 226, 283, 749
AspLEI GCGC 2 cut(s) 491, 553
AspS9I GGNCC 1 cut(s) 30
AsuC2I CCSGG 2 cut(s) 28, 686
AsuHPI GGTGA 3 cut(s) 113, 350, 658
AvaII GGWCC 1 cut(s) 30
BalI TGGCCA 1 cut(s) 307
BamHI GGATCC 1 cut(s) 460
BanII GRGCYC 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 3 cut(s) 213, 270, 761
BccI CCATC 2 cut(s) 384, 472
BceAI ACGGC 1 cut(s) 46
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 2 cut(s) 28, 686
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 1 cut(s) 179
BfmI CTRYAG 1 cut(s) 565
BfoI RGCGCY 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 796
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 3 cut(s) 227, 284, 750
BlpI GCTNAGC 2 cut(s) 155, 710
BlsI GCNGC 3 cut(s) 228, 285, 751
Bme1390I CCNGG 3 cut(s) 28, 555, 686
Bme18I GGWCC 1 cut(s) 30
BmeRI GACNNNNNGTC 1 cut(s) 255
BmgBI CACGTC 1 cut(s) 651
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 3 cut(s) 31, 462, 502
BmrFI CCNGG 3 cut(s) 28, 555, 686
BmsI GCATC 3 cut(s) 238, 400, 691
BpmI CTGGAG 1 cut(s) 504
Bpu1102I GCTNAGC 2 cut(s) 155, 710
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 2 cut(s) 28, 686
BsaJI CCNNGG 1 cut(s) 684
Bsc4I CCNNNNNNNGG 4 cut(s) 431, 470, 674, 729
Bse1I ACTGG 3 cut(s) 308, 480, 611
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 2 cut(s) 415, 464
BseLI CCNNNNNNNGG 4 cut(s) 431, 470, 674, 729
BseMII CTCAG 2 cut(s) 146, 724
BseNI ACTGG 3 cut(s) 308, 480, 611
BseXI GCAGC 3 cut(s) 213, 270, 761
BsgI GTGCAG 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 491
BshFI GGCC 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 3 cut(s) 27, 668, 686
BslFI GGGAC 2 cut(s) 43, 262
BslI CCNNNNNNNGG 4 cut(s) 431, 470, 674, 729
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 2 cut(s) 43, 262
BsnI GGCC 1 cut(s) 307
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 4 cut(s) 18, 135, 460, 591
Bsp1720I GCTNAGC 2 cut(s) 155, 710
BspACI CCGC 1 cut(s) 491
BspANI GGCC 1 cut(s) 307
BspCNI CTCAG 2 cut(s) 147, 723
BspFNI CGCG 1 cut(s) 491
BspHI TCATGA 2 cut(s) 169, 268
BspLI GGNNCC 3 cut(s) 31, 462, 502
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 4 cut(s) 130, 455, 468, 586
BsrI ACTGG 3 cut(s) 308, 480, 611
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 4 cut(s) 18, 135, 460, 591
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 43, 718
Bst6I CTCTTC 1 cut(s) 80
BstC8I GCNNGC 2 cut(s) 499, 747
BstDEI CTNAG 2 cut(s) 155, 710
BstF5I GGATG 2 cut(s) 415, 464
BstFNI CGCG 1 cut(s) 491
BstH2I RGCGCY 1 cut(s) 554
BstHHI GCGC 2 cut(s) 491, 553
BstKTI GATC 4 cut(s) 21, 138, 463, 594
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 4 cut(s) 18, 135, 460, 591
BstMWI GCNNNNNNNGC 4 cut(s) 67, 151, 507, 780
BstNI CCWGG 1 cut(s) 555
BstSCI CCNGG 3 cut(s) 26, 553, 684
BstSFI CTRYAG 1 cut(s) 565
BstUI CGCG 1 cut(s) 491
BstV1I GCAGC 3 cut(s) 213, 270, 761
BstX2I RGATCY 2 cut(s) 460, 591
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 2 cut(s) 460, 591
BsuRI GGCC 1 cut(s) 307
BtrI CACGTC 1 cut(s) 651
BtsCI GGATG 2 cut(s) 415, 464
BtsIMutI CAGTG 3 cut(s) 714, 752, 774
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 2 cut(s) 499, 747
CaiI CAGNNNCTG 1 cut(s) 554
CciI TCATGA 2 cut(s) 169, 268
CfoI GCGC 2 cut(s) 491, 553
Cfr13I GGNCC 1 cut(s) 30
CseI GACGC 1 cut(s) 173
Csp6I GTAC 2 cut(s) 24, 474
CviAII CATG 3 cut(s) 170, 269, 362
CviQI GTAC 2 cut(s) 24, 474
DdeI CTNAG 2 cut(s) 155, 710
DpnI GATC 4 cut(s) 20, 137, 462, 593
DpnII GATC 4 cut(s) 18, 135, 460, 591
DriI GACNNNNNGTC 1 cut(s) 255
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 1 cut(s) 80
Eam1105I GACNNNNNGTC 1 cut(s) 255
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 447
Eco47I GGWCC 1 cut(s) 30
Eco53kI GAGCTC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 776
EcoICRI GAGCTC 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 30
EcoRII CCWGG 1 cut(s) 553
EcoRV GATATC 1 cut(s) 447
EcoT22I ATGCAT 1 cut(s) 532
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 3 cut(s) 173, 272, 365
FalI AAGNNNNNCTT 2 cut(s) 729, 761
FaqI GGGAC 2 cut(s) 43, 262
FatI CATG 3 cut(s) 169, 268, 361
Fnu4HI GCNGC 3 cut(s) 227, 284, 750
FokI GGATG 2 cut(s) 422, 451
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 3 cut(s) 227, 284, 750
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 490, 552
GluI GCNGC 3 cut(s) 227, 284, 750
GsuI CTGGAG 1 cut(s) 504
HaeII RGCGCY 1 cut(s) 554
HaeIII GGCC 1 cut(s) 307
HapII CCGG 3 cut(s) 27, 668, 686
HgaI GACGC 1 cut(s) 173
HhaI GCGC 2 cut(s) 491, 553
Hin1II CATG 3 cut(s) 173, 272, 365
Hin6I GCGC 2 cut(s) 489, 551
HinP1I GCGC 2 cut(s) 489, 551
HinfI GANTC 3 cut(s) 272, 315, 694
HpaII CCGG 3 cut(s) 27, 668, 686
HphI GGTGA 3 cut(s) 113, 350, 658
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 140, 194, 801
Hpy188III TCNNGA 8 cut(s) 170, 269, 380, 407, 468, 521, 698, 838
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 335, 365, 733, 805
HpyCH4III ACNGT 2 cut(s) 43, 718
HpyCH4IV ACGT 3 cut(s) 213, 650, 796
HpyCH4V TGCA 8 cut(s) 229, 497, 530, 541, 658, 704, 749, 774
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 151, 507, 780
HpyF3I CTNAG 2 cut(s) 155, 710
HpySE526I ACGT 3 cut(s) 213, 650, 796
Hsp92II CATG 3 cut(s) 173, 272, 365
HspAI GCGC 2 cut(s) 489, 551
Kzo9I GATC 4 cut(s) 18, 135, 460, 591
LmnI GCTCC 3 cut(s) 506, 670, 688
Lsp1109I GCAGC 3 cut(s) 213, 270, 761
LweI GCATC 3 cut(s) 238, 400, 691
MaeI CTAG 1 cut(s) 179
MaeII ACGT 3 cut(s) 213, 650, 796
MaeIII GTNAC 2 cut(s) 544, 646
MalI GATC 4 cut(s) 20, 137, 462, 593
MboI GATC 4 cut(s) 18, 135, 460, 591
MboII GAAGA 7 cut(s) 97, 257, 381, 395, 769, 820, 842
MflI RGATCY 2 cut(s) 460, 591
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 307
MluCI AATT 1 cut(s) 605
MluNI TGGCCA 1 cut(s) 307
MlyI GAGTC 1 cut(s) 309
MmeI TCCRAC 2 cut(s) 412, 710
MnlI CCTC 6 cut(s) 202, 464, 528, 625, 668, 726
Mox20I TGGCCA 1 cut(s) 307
Mph1103I ATGCAT 1 cut(s) 532
MscI TGGCCA 1 cut(s) 307
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 1 cut(s) 307
MspI CCGG 3 cut(s) 27, 668, 686
MspR9I CCNGG 3 cut(s) 28, 555, 686
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 491
MwoI GCNNNNNNNGC 4 cut(s) 67, 151, 507, 780
NciI CCSGG 2 cut(s) 28, 686
NdeII GATC 4 cut(s) 18, 135, 460, 591
NlaIII CATG 3 cut(s) 173, 272, 365
NlaIV GGNNCC 3 cut(s) 31, 462, 502
NmuCI GTSAC 1 cut(s) 646
NsiI ATGCAT 1 cut(s) 532
PagI TCATGA 2 cut(s) 169, 268
PfeI GAWTC 2 cut(s) 272, 694
PkrI GCNGC 3 cut(s) 228, 285, 751
PleI GAGTC 1 cut(s) 309
PpsI GAGTC 1 cut(s) 309
PpuMI RGGWCCY 1 cut(s) 30
Psp124BI GAGCTC 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 3 cut(s) 31, 462, 502
PspPI GGNCC 1 cut(s) 30
PspPPI RGGWCCY 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 460, 591
RsaI GTAC 2 cut(s) 25, 475
RsaNI GTAC 2 cut(s) 24, 474
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 147
SatI GCNGC 3 cut(s) 227, 284, 750
Sau3AI GATC 4 cut(s) 18, 135, 460, 591
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 3 cut(s) 28, 555, 686
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 3 cut(s) 238, 400, 691
SfcI CTRYAG 1 cut(s) 565
SinI GGWCC 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 1 cut(s) 605
SsiI CCGC 1 cut(s) 491
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 147
StyD4I CCNGG 3 cut(s) 26, 553, 684
TaaI ACNGT 2 cut(s) 43, 718
TaiI ACGT 3 cut(s) 216, 653, 799
TaqI TCGA 3 cut(s) 147, 381, 449
TasI AATT 1 cut(s) 605
TfiI GAWTC 2 cut(s) 272, 694
TscAI CASTG 3 cut(s) 721, 759, 781
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 3 cut(s) 226, 283, 749
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 7 cut(s) 17, 107, 257, 285, 309, 633, 821
TspRI CASTG 3 cut(s) 721, 759, 781
VpaK11BI GGWCC 1 cut(s) 30
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.