pycom12g06280

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010029
Physical Location & Seq
Reverse (-)
6371576 .. 6374340
2765 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06280.1

Sequence Viewer

Length: 2667 bp
ATGGAGGTTTTGTATTTGTTTTGTGTTCTTCTTGTTGGGTTTCTATCCAAGTCTCATCTTGTGGCTGCTCAGCTTCATGATCAAGCTACATTGCTCGCCATTACCGAAGAGCTCGGAGTACTCGGATGGGGTGGCAACGGCTCAGATTACTGCTCTTGGCCCGGCATTGGCTGCGGTTTGAATCATTCCATGGTGGAAAAGCTTGATCTTTCGCACCAGAACCTCCGAGGTAATGTGACTCTTGTTTCCGAGCTCAAGGGTTTGAAGAGGCTTGACTTGTCGTACAATGATTTCCATGGACCAATTCCTTCAGCTTTTGGGAATTTGTCACAGCTTGAGTTTCTTGATTTGGCTTTGAATAAGTTTGGAGGTTCAATTCCGCCCGACCTCGGTAATCTCAGAAACCTTAGATCATTGAACCTCTCCAATAACTTCCTAGTAGAAGAAATACCTGATGAAATTCAGGGGCTAGAGAAGTTAGAGGATTTTCAAATTTCTAGTAATAAGTTGAGTGGTGTCGTCCCACTTTGGGTGGGGAATTTCACCAACCTGAGAGTTTTTACTGCCTATGAGAATGCATTTGAGGGTAAAATTCCAGATACTCTAGGCTCGGTTTCTAAGCTCGAATTGTTGAATCTGCACTCTAACCAGCTAGAAGGCCCAATTCCCAAAAGCATTTTTGCTTCAGGGAATCTGGAGTTTTTGGTTCTGACCCAGAACAGACTAGGTGGAGATATTCCTGAAGAAATTGGAAGCTGCAGTAGCCTTTCGAGTATTCGAATTGGTAACAATAATCTTATAGGTAGCATTCCTCATGCAATTGGAAATGTTAGTGGCCTTACATACTTTGAGGCAGACAATAACAATCTTTCTGGTGAGATTACTCCCGAGTTTTCCAAGTGCTCCAATATCACCCTCCTAAATTTAGCCTCCAATGGATTTACCGGGAAAATTCCTCCGGAGCTTGGCCAGCTCATGAATCTGCAGGAATTGATTCTCGCCGGCAACAGTCTGTTTGGAGATATTCCGAAATCAATTCTTGATTGCAAGAATCTTAACAAGCTTGATCTGAGCAATAACAGAATCAATGGTTCCATACCAAGTGATATCTGCAACATGTCGAGATTGCAATACTTGCTATTGGGTCAGAATTCGATTCGGGGGGAGATACCTCACGAGATTGGAAACTGTGTTAAACTGCTTGAGTTGCAGATGGGAAGAAACTATTTGACTGGCAGTATCCCTCCTGAAATTGGTCATATCAAAAACTTGCAGATTGCCTTAAACTTAAGCTTCAATCATCTCCGCGGACCTATTCCCGCGGATCTAGGGAAACTTGACAAGCTGGTTTCTCTCGATGTCTCCAACAATCAGCTCTCTGATGCTATTCCGACTGCACTTAAGGGCATGTTAAGCTTGATAGAGGTTAATTTCTCCAACAATCTGTTCACTGGCCCAGTACCAACATTCGTGCCATTCCAGAAGAGTTCGAATTCAAGTTTTCTTGGGAATAAAGGGCTTTGTGGAGAACCACTGAGCTCCTTATGTGAAAATTCTAACGGTGGTGACCGTGCAAATTATCATCATAGGGGTTCTTACAGGTTCATACTGGCAGTTATCGGCTCTGGTTTAGCAGTTTTTATATCGGTAACCATTGTTGTTTTGCTGTTTATGATAAGAGAGAGACAGGAGAAGGCAGAAAATGCTACAGGGACTGAAGACGAGGCAGCCAACAACCTACCGGTGATAGCAGCAGGCACTGTCTTTGTTGAGAATCTCAAACAAGCAATAGATCTTGATAGTGCTGTCAAAGCAACCATGAAGGCTTCAAACAAGATCAGCACCGGGACCTTTAGCACGGTTTACAAGGCTGTTATGCCGTCTGGGCTAATCATATCTGTGAAGAGACTGAAATCTATGGACAGGACAATCATTCATCACCAGAACAAGATGATCCGAGAGCTCGAAAGGCTGAGCAAACTCTGTCATGATAACCTAGTGCGTCCTATCGGATTTGTTATTTACGAGGACGTTGCTCTCTTGCTGCATCATTACTTGTCCTATGGGACATTATGTCAGCTTCTTCATGAATCCACAAAGCTGGCCGAATATGAACCTGACTGGCCAAAAAGACTCTCTATTGCCATAGGAGTAGCAGAAGGGTTGGCTTTCCTTCACCATGTAGCGATTATCCATCTCGATATATCTTCTGGTAATGTTCTTCTGGATGCTAACTCCAATCCTTTGGTTGGAGAGATCGAGATATCGAAGCTCTTGGATCCATCCAGAGGTACAGCCAGTATCAGCGCGGTTGCAGGCTCCTTTGGCTACATTCCTCCAGAATATGCATATACAATGCAAGTTACAGCGCCCGGAAATGTCTATAGCTATGGTGTTGTTTTGCTTGAGATCCTTACAACCAAATTACCAGTAGATGAAGCCTTTGACGAGGGGGTAGATTTGGTGACGTGGGTGCATAATGCTCCGGCAAGAGGCGAAACCCCGGAGCAGATTCTCGATGCAAGGCTCAGCACTGTGTCCTTCGGTTGGAGGAAAGAAATGCTTGCAGCACTGAAGATTGCTTTGCTCTGCACTGACAGCATACCCGCCAAACGTCCGAAAATGAAGAAGGTTGTGGAAATGCTGCAAGAAATCAAGAAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

889

Amino Acids

96.74

Weight (kDa)

5.7

Isoelectric Point (pI)

28.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 82 - 217 4.2e-09 Leucine-rich repeat region
LRR_14 PF23598 293 - 462 4.7e-11 Leucine-rich repeat region
LRR_8 PF13855 327 - 386 5.3e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 608 - 880 6.1e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 610 - 814 1.4e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 1308, 1322, 2309
AccIII TCCGGA 1 cut(s) 958
AciI CCGC 8 cut(s) 174, 380, 1306, 1308, 1320, 1322, 2309, 2607
AclWI GGATC 5 cut(s) 1332, 1948, 2273, 2286, 2404
AcoI YGGCCR 3 cut(s) 967, 2103, 2123
AcuI CTGAAG 5 cut(s) 294, 669, 762, 1737, 2594
AfaI GTAC 4 cut(s) 120, 284, 1461, 2293
AfiI CCNNNNNNNGG 9 cut(s) 167, 389, 529, 965, 1253, 2249, 2288, 2492, 2547
AflII CTTAAG 2 cut(s) 1288, 1400
AflIII ACRYGT 1 cut(s) 1116
AgeI ACCGGT 1 cut(s) 1741
AhdI GACNNNNNGTC 1 cut(s) 2073
AjiI CACGTC 1 cut(s) 2469
AjuI GAANNNNNNNTTGG 2 cut(s) 1430, 1462
Alw21I GWGCWC 5 cut(s) 114, 255, 905, 1541, 1965
Alw26I GTCTC 4 cut(s) 57, 1366, 1678, 1900
AlwI GGATC 5 cut(s) 1332, 1948, 2273, 2286, 2404
AlwNI CAGNNNCTG 2 cut(s) 1715, 1760
Ama87I CYCGRG 1 cut(s) 887
Aor13HI TCCGGA 1 cut(s) 958
AoxI GGCC 7 cut(s) 158, 658, 835, 967, 1453, 2103, 2123
ApeKI GCWGC 8 cut(s) 65, 171, 756, 1727, 1751, 2044, 2567, 2644
AsiGI ACCGGT 1 cut(s) 1741
Asp700I GAANNNNTTC 1 cut(s) 993
AspLEI GCGC 2 cut(s) 2309, 2371
AspS9I GGNCC 6 cut(s) 159, 299, 659, 1310, 1454, 1848
AsuC2I CCSGG 5 cut(s) 162, 946, 1846, 2373, 2504
AsuHPI GGTGA 8 cut(s) 535, 887, 904, 1577, 1756, 1931, 2168, 2476
AsuII TTCGAA 2 cut(s) 778, 1490
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 3 cut(s) 299, 1310, 1848
BalI TGGCCA 2 cut(s) 969, 2125
BamHI GGATCC 1 cut(s) 2278
BanII GRGCYC 4 cut(s) 114, 255, 1541, 1965
BauI CACGAG 1 cut(s) 1175
BbsI GAAGAC 1 cut(s) 1725
Bbv12I GWGCWC 5 cut(s) 114, 255, 905, 1541, 1965
BbvI GCAGC 8 cut(s) 52, 158, 743, 1739, 1763, 2031, 2579, 2631
BccI CCATC 4 cut(s) 120, 1207, 2202, 2290
BceAI ACGGC 2 cut(s) 154, 1864
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 5 cut(s) 162, 946, 1846, 2373, 2504
BcoDI GTCTC 4 cut(s) 57, 1366, 1678, 1900
BfaI CTAG 8 cut(s) 437, 470, 498, 605, 653, 725, 1328, 1997
BfmI CTRYAG 4 cut(s) 757, 983, 1707, 2383
BfoI RGCGCY 1 cut(s) 2372
BfrI CTTAAG 2 cut(s) 1288, 1400
BfuI GTATCC 1 cut(s) 1250
BglI GCCNNNNNGGC 1 cut(s) 1885
BglII AGATCT 1 cut(s) 1792
BisI GCNGC 8 cut(s) 66, 172, 757, 1728, 1752, 2045, 2568, 2645
BlpI GCTNAGC 3 cut(s) 69, 1973, 2528
BlsI GCNGC 8 cut(s) 67, 173, 758, 1729, 1753, 2046, 2569, 2646
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 5 cut(s) 162, 946, 1846, 2373, 2504
Bme18I GGWCC 3 cut(s) 299, 1310, 1848
BmeRI GACNNNNNGTC 1 cut(s) 2073
BmeT110I CYCGRG 1 cut(s) 887
BmgBI CACGTC 1 cut(s) 2469
BmgT120I GGNCC 6 cut(s) 159, 299, 659, 1310, 1454, 1848
BmiI GGNNCC 4 cut(s) 1093, 1849, 2280, 2320
BmrFI CCNGG 5 cut(s) 162, 946, 1846, 2373, 2504
BmrI ACTGGG 1 cut(s) 1451
BmsI GCATC 4 cut(s) 1372, 2056, 2218, 2509
BmuI ACTGGG 1 cut(s) 1451
BpiI GAAGAC 1 cut(s) 1725
BpmI CTGGAG 2 cut(s) 716, 2322
Bpu1102I GCTNAGC 3 cut(s) 69, 1973, 2528
Bpu14I TTCGAA 2 cut(s) 778, 1490
BpuEI CTTGAG 4 cut(s) 239, 356, 1223, 2426
BpuMI CCSGG 5 cut(s) 162, 946, 1846, 2373, 2504
BsaJI CCNNGG 7 cut(s) 189, 226, 295, 388, 1306, 1320, 2502
BsaWI WCCGGW 2 cut(s) 958, 1741
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 9 cut(s) 167, 389, 529, 965, 1253, 2249, 2288, 2492, 2547
Bse118I RCCGGY 2 cut(s) 1001, 1741
Bse1I ACTGG 7 cut(s) 1237, 1456, 1457, 1614, 2126, 2298, 2429
Bse3DI GCAATG 1 cut(s) 89
BseAI TCCGGA 1 cut(s) 958
BseDI CCNNGG 7 cut(s) 189, 226, 295, 388, 1306, 1320, 2502
BseGI GGATG 3 cut(s) 131, 2233, 2282
BseLI CCNNNNNNNGG 9 cut(s) 167, 389, 529, 965, 1253, 2249, 2288, 2492, 2547
BseMI GCAATG 1 cut(s) 89
BseMII CTCAG 8 cut(s) 83, 156, 412, 542, 1061, 1526, 1964, 2542
BseNI ACTGG 7 cut(s) 1237, 1456, 1457, 1614, 2126, 2298, 2429
BseXI GCAGC 8 cut(s) 52, 158, 743, 1739, 1763, 2031, 2579, 2631
BsgI GTGCAG 3 cut(s) 623, 1380, 2575
Bsh1236I CGCG 3 cut(s) 1308, 1322, 2309
BshFI GGCC 7 cut(s) 160, 660, 837, 969, 1455, 2105, 2125
BshTI ACCGGT 1 cut(s) 1741
BsiHKAI GWGCWC 5 cut(s) 114, 255, 905, 1541, 1965
BsiHKCI CYCGRG 1 cut(s) 887
BsiSI CCGG 9 cut(s) 162, 945, 959, 1002, 1742, 1845, 2373, 2486, 2504
BslFI GGGAC 4 cut(s) 506, 1726, 1861, 2080
BslI CCNNNNNNNGG 9 cut(s) 167, 389, 529, 965, 1253, 2249, 2288, 2492, 2547
BsmAI GTCTC 4 cut(s) 57, 1366, 1678, 1900
BsmFI GGGAC 4 cut(s) 506, 1726, 1861, 2080
BsmI GAATGC 2 cut(s) 580, 807
BsnI GGCC 7 cut(s) 160, 660, 837, 969, 1455, 2105, 2125
BsoBI CYCGRG 1 cut(s) 887
Bsp119I TTCGAA 2 cut(s) 778, 1490
Bsp1286I GDGCHC 5 cut(s) 114, 255, 905, 1541, 1965
Bsp13I TCCGGA 1 cut(s) 958
Bsp1720I GCTNAGC 3 cut(s) 69, 1973, 2528
Bsp19I CCATGG 2 cut(s) 189, 295
BspACI CCGC 8 cut(s) 174, 380, 1306, 1308, 1320, 1322, 2309, 2607
BspANI GGCC 7 cut(s) 160, 660, 837, 969, 1455, 2105, 2125
BspCNI CTCAG 8 cut(s) 82, 155, 411, 543, 1062, 1527, 1965, 2541
BspEI TCCGGA 1 cut(s) 958
BspFNI CGCG 3 cut(s) 1308, 1322, 2309
BspHI TCATGA 4 cut(s) 76, 975, 1987, 2086
BspLI GGNNCC 4 cut(s) 1093, 1849, 2280, 2320
BspMAI CTGCAG 2 cut(s) 761, 987
BspPI GGATC 5 cut(s) 1332, 1948, 2273, 2286, 2404
BspQI GCTCTTC 1 cut(s) 102
BspT104I TTCGAA 2 cut(s) 778, 1490
BspTI CTTAAG 2 cut(s) 1288, 1400
BsrDI GCAATG 1 cut(s) 89
BsrFI RCCGGY 2 cut(s) 1001, 1741
BsrI ACTGG 7 cut(s) 1237, 1456, 1457, 1614, 2126, 2298, 2429
BssAI RCCGGY 2 cut(s) 1001, 1741
BssECI CCNNGG 7 cut(s) 189, 226, 295, 388, 1306, 1320, 2502
BssSI CACGAG 1 cut(s) 1175
BssT1I CCWWGG 2 cut(s) 189, 295
Bst2BI CACGAG 1 cut(s) 1175
Bst4CI ACNGT 7 cut(s) 1010, 1190, 1562, 1571, 1762, 1861, 2536
Bst6I CTCTTC 4 cut(s) 102, 260, 1478, 1898
BstAFI CTTAAG 2 cut(s) 1288, 1400
BstAPI GCANNNNNTGC 3 cut(s) 171, 1135, 1703
BstBI TTCGAA 2 cut(s) 778, 1490
BstC8I GCNNGC 7 cut(s) 96, 971, 1003, 1756, 2103, 2317, 2565
BstDSI CCRYGG 4 cut(s) 189, 295, 1306, 1320
BstEII GGTNACC 2 cut(s) 1565, 1648
BstF5I GGATG 3 cut(s) 131, 2233, 2282
BstFNI CGCG 3 cut(s) 1308, 1322, 2309
BstH2I RGCGCY 1 cut(s) 2372
BstHHI GCGC 2 cut(s) 2309, 2371
BstMAI GTCTC 4 cut(s) 57, 1366, 1678, 1900
BstNSI RCATGY 2 cut(s) 1120, 1411
BstPI GGTNACC 2 cut(s) 1565, 1648
BstSCI CCNGG 5 cut(s) 160, 944, 1844, 2371, 2502
BstSFI CTRYAG 4 cut(s) 757, 983, 1707, 2383
BstUI CGCG 3 cut(s) 1308, 1322, 2309
BstV1I GCAGC 8 cut(s) 52, 158, 743, 1739, 1763, 2031, 2579, 2631
BstV2I GAAGAC 1 cut(s) 1725
BstX2I RGATCY 4 cut(s) 1324, 1792, 2278, 2409
BstXI CCANNNNNNTGG 2 cut(s) 2101, 2245
BstYI RGATCY 4 cut(s) 1324, 1792, 2278, 2409
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 7 cut(s) 160, 660, 837, 969, 1455, 2105, 2125
BtgI CCRYGG 4 cut(s) 189, 295, 1306, 1320
BtrI CACGTC 1 cut(s) 2469
BtsCI GGATG 3 cut(s) 131, 2233, 2282
BtsIMutI CAGTG 6 cut(s) 1449, 1532, 1758, 2532, 2570, 2592
Cac8I GCNNGC 7 cut(s) 96, 971, 1003, 1756, 2103, 2317, 2565
CaiI CAGNNNCTG 2 cut(s) 1715, 1760
CciI TCATGA 4 cut(s) 76, 975, 1987, 2086
CfoI GCGC 2 cut(s) 2309, 2371
Cfr10I RCCGGY 2 cut(s) 1001, 1741
Cfr13I GGNCC 6 cut(s) 159, 299, 659, 1310, 1454, 1848
Cfr42I CCGCGG 2 cut(s) 1309, 1323
CseI GACGC 1 cut(s) 1991
Csp6I GTAC 4 cut(s) 119, 283, 1460, 2292
CspAI ACCGGT 1 cut(s) 1741
CviQI GTAC 4 cut(s) 119, 283, 1460, 2292
DriI GACNNNNNGTC 1 cut(s) 2073
EaeI YGGCCR 3 cut(s) 967, 2103, 2123
Eam1104I CTCTTC 4 cut(s) 102, 260, 1478, 1898
Eam1105I GACNNNNNGTC 1 cut(s) 2073
EarI CTCTTC 4 cut(s) 102, 260, 1478, 1898
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 4 cut(s) 112, 253, 1539, 1963
Eco130I CCWWGG 2 cut(s) 189, 295
Eco24I GRGCYC 4 cut(s) 114, 255, 1541, 1965
Eco32I GATATC 2 cut(s) 1108, 2265
Eco47I GGWCC 3 cut(s) 299, 1310, 1848
Eco53kI GAGCTC 4 cut(s) 112, 253, 1539, 1963
Eco57I CTGAAG 5 cut(s) 294, 669, 762, 1737, 2594
Eco88I CYCGRG 1 cut(s) 887
Eco91I GGTNACC 2 cut(s) 1565, 1648
EcoICRI GAGCTC 4 cut(s) 112, 253, 1539, 1963
EcoO109I RGGNCCY 1 cut(s) 1848
EcoO65I GGTNACC 2 cut(s) 1565, 1648
EcoRI GAATTC 2 cut(s) 1150, 1492
EcoRV GATATC 2 cut(s) 1108, 2265
EcoT14I CCWWGG 2 cut(s) 189, 295
EcoT22I ATGCAT 2 cut(s) 580, 2350
EcoT38I GRGCYC 4 cut(s) 114, 255, 1541, 1965
ErhI CCWWGG 2 cut(s) 189, 295
FalI AAGNNNNNCTT 2 cut(s) 2547, 2579
FaqI GGGAC 4 cut(s) 506, 1726, 1861, 2080
FauI CCCGC 2 cut(s) 1327, 2614
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 8 cut(s) 66, 172, 757, 1728, 1752, 2045, 2568, 2645
FokI GGATG 3 cut(s) 138, 2240, 2269
FriOI GRGCYC 4 cut(s) 114, 255, 1541, 1965
Fsp4HI GCNGC 8 cut(s) 66, 172, 757, 1728, 1752, 2045, 2568, 2645
FspBI CTAG 8 cut(s) 437, 470, 498, 605, 653, 725, 1328, 1997
GlaI GCGC 2 cut(s) 2308, 2370
GluI GCNGC 8 cut(s) 66, 172, 757, 1728, 1752, 2045, 2568, 2645
GsuI CTGGAG 2 cut(s) 716, 2322
HaeII RGCGCY 1 cut(s) 2372
HaeIII GGCC 7 cut(s) 160, 660, 837, 969, 1455, 2105, 2125
HapII CCGG 9 cut(s) 162, 945, 959, 1002, 1742, 1845, 2373, 2486, 2504
HgaI GACGC 1 cut(s) 1991
HhaI GCGC 2 cut(s) 2309, 2371
Hin6I GCGC 2 cut(s) 2307, 2369
HinP1I GCGC 2 cut(s) 2307, 2369
HindIII AAGCTT 4 cut(s) 200, 1061, 1291, 1414
HpaII CCGG 9 cut(s) 162, 945, 959, 1002, 1742, 1845, 2373, 2486, 2504
HphI GGTGA 8 cut(s) 535, 887, 904, 1577, 1756, 1931, 2168, 2476
Hpy166II GTNNAC 2 cut(s) 1449, 1864
Hpy8I GTNNAC 2 cut(s) 1449, 1864
HpyAV CCTTC 8 cut(s) 318, 650, 1687, 1816, 2153, 2183, 2551, 2623
HpyCH4III ACNGT 7 cut(s) 1010, 1190, 1562, 1571, 1762, 1861, 2536
HpyCH4IV ACGT 3 cut(s) 2031, 2468, 2614
HpySE526I ACGT 3 cut(s) 2031, 2468, 2614
HspAI GCGC 2 cut(s) 2307, 2369
Kpn2I TCCGGA 1 cut(s) 958
KroI GCCGGC 1 cut(s) 1001
KroNI GCCGGC 1 cut(s) 1003
Ksp22I TGATCA 1 cut(s) 79
KspI CCGCGG 2 cut(s) 1309, 1323
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 6 cut(s) 908, 961, 1544, 2324, 2488, 2506
Lsp1109I GCAGC 8 cut(s) 52, 158, 743, 1739, 1763, 2031, 2579, 2631
LweI GCATC 4 cut(s) 1372, 2056, 2218, 2509
MaeI CTAG 8 cut(s) 437, 470, 498, 605, 653, 725, 1328, 1997
MaeII ACGT 3 cut(s) 2031, 2468, 2614
MaeIII GTNAC 7 cut(s) 235, 327, 785, 1565, 1648, 2362, 2464
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 4 cut(s) 1324, 1792, 2278, 2409
MhlI GDGCHC 5 cut(s) 114, 255, 905, 1541, 1965
MlsI TGGCCA 2 cut(s) 969, 2125
MluNI TGGCCA 2 cut(s) 969, 2125
MlyI GAGTC 2 cut(s) 232, 2127
MmeI TCCRAC 5 cut(s) 1389, 1415, 1461, 2230, 2528
Mox20I TGGCCA 2 cut(s) 969, 2125
Mph1103I ATGCAT 2 cut(s) 580, 2350
MroI TCCGGA 1 cut(s) 958
MroNI GCCGGC 1 cut(s) 1001
MroXI GAANNNNTTC 1 cut(s) 993
MscI TGGCCA 2 cut(s) 969, 2125
MseI TTAA 7 cut(s) 1056, 1194, 1283, 1289, 1401, 1412, 1428
MslI CAYNNNNRTG 1 cut(s) 1898
Msp20I TGGCCA 2 cut(s) 969, 2125
MspA1I CMGCKG 2 cut(s) 1308, 1322
MspCI CTTAAG 2 cut(s) 1288, 1400
MspI CCGG 9 cut(s) 162, 945, 959, 1002, 1742, 1845, 2373, 2486, 2504
MspR9I CCNGG 5 cut(s) 162, 946, 1846, 2373, 2504
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 2 cut(s) 580, 807
MvnI CGCG 3 cut(s) 1308, 1322, 2309
NaeI GCCGGC 1 cut(s) 1003
NciI CCSGG 5 cut(s) 162, 946, 1846, 2373, 2504
NcoI CCATGG 2 cut(s) 189, 295
NgoMIV GCCGGC 1 cut(s) 1001
NlaIV GGNNCC 4 cut(s) 1093, 1849, 2280, 2320
NmuCI GTSAC 4 cut(s) 235, 327, 1565, 2464
NsiI ATGCAT 2 cut(s) 580, 2350
NspI RCATGY 2 cut(s) 1120, 1411
NspV TTCGAA 2 cut(s) 778, 1490
PagI TCATGA 4 cut(s) 76, 975, 1987, 2086
PciI ACATGT 1 cut(s) 1116
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 2 cut(s) 580, 807
PdiI GCCGGC 1 cut(s) 1003
PdmI GAANNNNTTC 1 cut(s) 993
PinAI ACCGGT 1 cut(s) 1741
PkrI GCNGC 8 cut(s) 67, 173, 758, 1729, 1753, 2046, 2569, 2646
PleI GAGTC 2 cut(s) 232, 2127
PpsI GAGTC 2 cut(s) 232, 2127
PpuMI RGGWCCY 1 cut(s) 1848
PscI ACATGT 1 cut(s) 1116
Psp124BI GAGCTC 4 cut(s) 114, 255, 1541, 1965
Psp5II RGGWCCY 1 cut(s) 1848
PspEI GGTNACC 2 cut(s) 1565, 1648
PspN4I GGNNCC 4 cut(s) 1093, 1849, 2280, 2320
PspPI GGNCC 6 cut(s) 159, 299, 659, 1310, 1454, 1848
PspPPI RGGWCCY 1 cut(s) 1848
PstI CTGCAG 2 cut(s) 761, 987
PstNI CAGNNNCTG 2 cut(s) 1715, 1760
PsuI RGATCY 4 cut(s) 1324, 1792, 2278, 2409
RsaI GTAC 4 cut(s) 120, 284, 1461, 2293
RsaNI GTAC 4 cut(s) 119, 283, 1460, 2292
RseI CAYNNNNRTG 1 cut(s) 1898
SacI GAGCTC 4 cut(s) 114, 255, 1541, 1965
SacII CCGCGG 2 cut(s) 1309, 1323
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 7 cut(s) 1056, 1194, 1283, 1289, 1401, 1412, 1428
SatI GCNGC 8 cut(s) 66, 172, 757, 1728, 1752, 2045, 2568, 2645
Sau96I GGNCC 6 cut(s) 159, 299, 659, 1310, 1454, 1848
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 2 cut(s) 232, 2127
ScrFI CCNGG 5 cut(s) 162, 946, 1846, 2373, 2504
SduI GDGCHC 5 cut(s) 114, 255, 905, 1541, 1965
SfaNI GCATC 4 cut(s) 1372, 2056, 2218, 2509
SfcI CTRYAG 4 cut(s) 757, 983, 1707, 2383
Sfr303I CCGCGG 2 cut(s) 1309, 1323
SfuI TTCGAA 2 cut(s) 778, 1490
SgrBI CCGCGG 2 cut(s) 1309, 1323
SinI GGWCC 3 cut(s) 299, 1310, 1848
SmiMI CAYNNNNRTG 1 cut(s) 1898
SmlI CTYRAG 6 cut(s) 254, 335, 1202, 1288, 1400, 2405
SmoI CTYRAG 6 cut(s) 254, 335, 1202, 1288, 1400, 2405
SsiI CCGC 8 cut(s) 174, 380, 1306, 1308, 1320, 1322, 2309, 2607
SspMI CTAG 8 cut(s) 437, 470, 498, 605, 653, 725, 1328, 1997
SstI GAGCTC 4 cut(s) 114, 255, 1541, 1965
StyD4I CCNGG 5 cut(s) 160, 944, 1844, 2371, 2502
StyI CCWWGG 2 cut(s) 189, 295
TaaI ACNGT 7 cut(s) 1010, 1190, 1562, 1571, 1762, 1861, 2536
TaiI ACGT 3 cut(s) 2034, 2471, 2617
TatI WGTACW 1 cut(s) 118
Tru1I TTAA 7 cut(s) 1056, 1194, 1283, 1289, 1401, 1412, 1428
Tru9I TTAA 7 cut(s) 1056, 1194, 1283, 1289, 1401, 1412, 1428
TscAI CASTG 6 cut(s) 1456, 1539, 1765, 2539, 2577, 2599
TseFI GTSAC 4 cut(s) 235, 327, 1565, 2464
TseI GCWGC 8 cut(s) 65, 171, 756, 1727, 1751, 2044, 2567, 2644
Tsp45I GTSAC 4 cut(s) 235, 327, 1565, 2464
TspRI CASTG 6 cut(s) 1456, 1539, 1765, 2539, 2577, 2599
Vha464I CTTAAG 2 cut(s) 1288, 1400
VpaK11BI GGWCC 3 cut(s) 299, 1310, 1848
XceI RCATGY 2 cut(s) 1120, 1411
XmnI GAANNNNTTC 1 cut(s) 993
XspI CTAG 8 cut(s) 437, 470, 498, 605, 653, 725, 1328, 1997
ZrmI AGTACT 1 cut(s) 120
Zsp2I ATGCAT 2 cut(s) 580, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.