MD14G1071100.v1.1

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010029
Physical Location & Seq
Reverse (-)
7810684 .. 7814619
3936 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1071100.v1.1.491

Sequence Viewer

Length: 2667 bp
ATGGTGCTTTTGTGCTTGTTCTGTGTTCTTCTTGTTGGGTTTCTATCCGAGTCCCATCTTGTGGCTGCTCAGCTTTATGATCAAGCTACATTGCTCGCCATTACCGAAGAGCTCGGAGTACTCGGATGGGGTGGCAACAGTTCAGATTACTGCTCTTGGCCCGGCATTGGATGCGGTTTAAACCACTCTATGGTCGAAAAGCTTGATCTTTCGCACCACAACCTCCGAGGTAATGTGACTCTTGTTTCTCAGCTCAAGGCTCTGAAGAGGCTTGACCTCTCTTACAATGATTTCCATGGATCAATTCCTTTAGCTTTTGGAAATTTGTCACAGCTTGAATTTCTTGATTTGTCTTTGAATAAGTTCGGAGGTTCAATTCCGCCGGAGCTGGGTAAACTCAGAAACCTTAGATCATTGAACCTCTCCAATAACTTTCTTGTAGGAAAAATACCTGATGAAATTCAGGGCCTAGAAAAGTTAGAGAATTTTCAAATTTCAAGTAATAAGTTGAGTGGTGTTATCCCTATGTGGGTGGGGAATTTCACCAACTTGAGAGTTTTTACTGCCTATGAGAATGCATTGGAGGGTAAAATCCCAGATAATCTAGGGTCGCTTTCTGAGCTCGAATCATTGAATCTGCATTCTAACCAGCTAGAAGGCCCAATTCCCAAAAGCATTTTTGCTTCAGGGAAGCTGGAGTTTTTGGTTCTGACCCAGAACAGACTAAGTGGGGATATTCCTGAAGAAATTGGAAACTGCAGTAGCCTTTCGAGTATTCGAATTGGTAACAATGATCTTGTAGGTAGCATTCCTCATGCAATTGGGAATGTTAGTAGCCTTACATACTTTGAGGCTGACAATAACAATCTTTCTGGTGAGATTGCTCCAGAGTTTTCTAAGTGTTCCAATCTCACCCTCTTAAATTTAGCCTCAAATGGATTTACCGGGAGAATTCCTCCGGAGCTTGGCCAGCTCATGAATCTGCAGGAATTAATTCTCTCCGACAACAGTCTGTTTGGAGATATTCCGAAATCAATTCTCGATTGCAAGAATCTTAACAAGCTTGATCTGACCAATAATAGAATCAATGGTACCATACCAAGTGATATCTGCAACATGTCGAGATTGCAATACTTGCTGTTGGGTCAGAATTCGATACGGGGTGAAATACCTCATGAGATTGCAAACTGTGTTAAACTGCTTGAGTTGCAGATGGGAAGAAACTATTTCACTGGCAGTATCCCTCCGGAAATTGGTCATATCAAAAACTTACAGATTGCCTTAAACTTAAGCTTCAATCATCTCCATGGACCTATTCCTGCAGATTTAGGGAAACTTGATAAGCTGGTTTCTCTTGATGTCTCCAACAATCAGCTGTCTGATGTCATTCCAACTGCACTCAAGGGCATGTTAAGCTTGATAGAGGTTAATTTCTCAAACAATCTGTTCACTGGCCAGATACCAACATTCGTGCCATTCCAGAAGAGTCCGAATACAAGTTTTATTGGGAATAAAGGGCTTTGTGGAGAACCACTGAGCTCCTTATGTGCAAATGCTAATGGTGGTGACCGTGCAAGTTACCATCACAGGGTTTCTTACAGGATTGTACTGGCAGTTATCGGTTCTGGTTTGGCAGTTTTTATATCGGTAACCATTGTTGTCCTGCTGTTTATGCTCAGAGAGAGACAGGAGAAGGCAGAAAAAGCTGCAGGGACTGAAGACGAGGCAGCCAACAACCTACCGGCGATAGCAGCAGGCAATATCTTTGTTGAGAATCTCAAACAAGCAATAGATCTTGATTGTGCTGTTAAAGCAACCATGAAGGATTCAAACAAGATCAGTACTGGGACTTTTAGCACAGTTTACAAGGCTGTTATGCCATCGGGGCTGATTATATCAGTGAAGAGACTGAAATCTATGGACAGGACAATCATTCATCACCAGAACAAGATGATTCGAGAGCTCGAAAGGTTGAGCAAACTCTGTCATGACAACCTAGTGCGTCCTATCGGATTTGTTATTTACGAGGATGTTGCTCTTTTGCTGCATCATTACTTACCCTATGGGACATTATGTCAGCTTCTTCATGAATCCACCAAGCTGCCCGAATATGAACCTGACTGGCCCAAAAGACTCTCTATTGCCATTGGAGTAGCAGAAGGATTGACTTTCCTTCACCATGTGGCTATTATCCACCTCGATATTTCTACTGGTAATGTTCTTTTGGATGCTAACTTCAAGCCTTTGGTCGGAGAGATTGAGATCTCGAAGCTCTTGGATCCAACCAGAGGCACAGCCAGTATCAGTGCAGTTGCAGGCTCCTTTGGCTACATTCCTCCGGAATATGCATATACAATGCAAGTTACAGCTCCTGGGAACGTCTATAGCTACGGTGTTGTTTTGCTTGAGATCCTTACAACCAAATTACCAGTAGATGAAGCCTTTGATGAGGGGGTAGATTTGGTGAAGTGGGTGCATAATGCTCCCGCAAGAGGCGAAACCCCAGAGCAGATTCTCGATGAAAGGCTCAGCACAGTGTCCTTCGGTTGGAGGAAAGAAATGCTTGCAGCACTGAAGATTGCATTGCTCTGCACTGACAGCATACCCGCCAAACGTCCAAAAATGAAGAAGGTAGTGGAAATGCTGCAAGAAATAAAGAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

889

Amino Acids

97.26

Weight (kDa)

5.92

Isoelectric Point (pI)

27.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 80 - 217 1.4e-11 Leucine-rich repeat region
LRR_14 PF23598 277 - 462 1e-11 Leucine-rich repeat region
LRR_8 PF13855 327 - 386 8.9e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 610 - 880 9.4e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 610 - 816 6.8e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 2 cut(s) 61, 190
AccIII TCCGGA 3 cut(s) 958, 1246, 2338
AciI CCGC 4 cut(s) 174, 380, 2487, 2607
AclWI GGATC 4 cut(s) 307, 2273, 2286, 2404
AcoI YGGCCR 2 cut(s) 967, 1453
AcsI RAATTY 9 cut(s) 322, 338, 459, 484, 492, 538, 922, 951, 1150
AcuI CTGAAG 5 cut(s) 284, 669, 762, 1737, 2594
AdeI CACNNNGTG 1 cut(s) 2182
AfaI GTAC 4 cut(s) 120, 1093, 1608, 1843
AflII CTTAAG 1 cut(s) 1288
AflIII ACRYGT 1 cut(s) 1116
AhdI GACNNNNNGTC 1 cut(s) 2073
AjnI CCWGG 1 cut(s) 2371
Alw21I GWGCWC 4 cut(s) 114, 624, 1541, 1965
Alw26I GTCTC 3 cut(s) 1366, 1678, 1900
AlwI GGATC 4 cut(s) 307, 2273, 2286, 2404
AlwNI CAGNNNCTG 2 cut(s) 1715, 2372
Aor13HI TCCGGA 3 cut(s) 958, 1246, 2338
AoxI GGCC 6 cut(s) 158, 466, 658, 967, 1453, 2123
ApeKI GCWGC 8 cut(s) 65, 1706, 1727, 1751, 2044, 2101, 2567, 2644
ApoI RAATTY 9 cut(s) 322, 338, 459, 484, 492, 538, 922, 951, 1150
AseI ATTAAT 1 cut(s) 992
Asp700I GAANNNNTTC 2 cut(s) 362, 993
Asp718I GGTACC 1 cut(s) 1091
AspS9I GGNCC 5 cut(s) 159, 466, 659, 1310, 2124
AsuC2I CCSGG 2 cut(s) 162, 946
AsuHPI GGTGA 8 cut(s) 535, 887, 904, 1175, 1577, 1931, 2168, 2476
AsuII TTCGAA 1 cut(s) 778
AvaII GGWCC 1 cut(s) 1310
BalI TGGCCA 2 cut(s) 969, 1455
BamHI GGATCC 1 cut(s) 2278
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 4 cut(s) 114, 624, 1541, 1965
BbsI GAAGAC 1 cut(s) 1725
Bbv12I GWGCWC 4 cut(s) 114, 624, 1541, 1965
BbvI GCAGC 8 cut(s) 52, 1693, 1739, 1763, 2031, 2088, 2579, 2631
BccI CCATC 5 cut(s) 63, 120, 1207, 1590, 1888
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciT130I CCWGG 1 cut(s) 2373
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 162, 946
BcoDI GTCTC 3 cut(s) 1366, 1678, 1900
BfaI CTAG 4 cut(s) 470, 605, 653, 1997
BfmI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
BfrI CTTAAG 1 cut(s) 1288
BfuI GTATCC 1 cut(s) 1250
BglI GCCNNNNNGGC 1 cut(s) 1885
BglII AGATCT 2 cut(s) 1792, 2262
BisI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
BlpI GCTNAGC 2 cut(s) 69, 2528
BlsI GCNGC 8 cut(s) 67, 1708, 1729, 1753, 2046, 2103, 2569, 2646
BmcAI AGTACT 2 cut(s) 120, 1843
Bme1390I CCNGG 3 cut(s) 162, 946, 2373
Bme18I GGWCC 1 cut(s) 1310
BmeRI GACNNNNNGTC 1 cut(s) 2073
BmgT120I GGNCC 5 cut(s) 159, 466, 659, 1310, 2124
BmiI GGNNCC 3 cut(s) 1093, 2280, 2320
BmrFI CCNGG 3 cut(s) 162, 946, 2373
BmrI ACTGGG 1 cut(s) 1854
BmsI GCATC 3 cut(s) 161, 2056, 2218
BmuI ACTGGG 1 cut(s) 1854
BoxI GACNNNNGTC 1 cut(s) 1008
BpiI GAAGAC 1 cut(s) 1725
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 2 cut(s) 716, 870
Bpu1102I GCTNAGC 2 cut(s) 69, 2528
Bpu14I TTCGAA 1 cut(s) 778
BpuEI CTTGAG 5 cut(s) 239, 571, 1223, 1385, 2426
BpuMI CCSGG 2 cut(s) 162, 946
BsaBI GATNNNNATC 1 cut(s) 2261
BsaJI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BsaWI WCCGGW 3 cut(s) 958, 1246, 2338
Bse118I RCCGGY 1 cut(s) 1741
Bse1I ACTGG 8 cut(s) 1237, 1456, 1614, 1849, 2126, 2215, 2298, 2429
Bse3DI GCAATG 2 cut(s) 89, 2582
Bse8I GATNNNNATC 1 cut(s) 2261
BseAI TCCGGA 3 cut(s) 958, 1246, 2338
BseBI CCWGG 1 cut(s) 2373
BseDI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BseGI GGATG 4 cut(s) 131, 176, 2035, 2233
BseJI GATNNNNATC 1 cut(s) 2261
BseMI GCAATG 2 cut(s) 89, 2582
BseMII CTCAG 7 cut(s) 83, 263, 412, 609, 1526, 1690, 2542
BseNI ACTGG 8 cut(s) 1237, 1456, 1614, 1849, 2126, 2215, 2298, 2429
BseXI GCAGC 8 cut(s) 52, 1693, 1739, 1763, 2031, 2088, 2579, 2631
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 3 cut(s) 1380, 2328, 2575
BshFI GGCC 6 cut(s) 160, 468, 660, 969, 1455, 2125
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 4 cut(s) 114, 624, 1541, 1965
BsiSI CCGG 7 cut(s) 162, 383, 945, 959, 1247, 1742, 2339
BslFI GGGAC 4 cut(s) 37, 1726, 1861, 2080
BsmAI GTCTC 3 cut(s) 1366, 1678, 1900
BsmFI GGGAC 4 cut(s) 37, 1726, 1861, 2080
BsmI GAATGC 3 cut(s) 580, 640, 807
BsnI GGCC 6 cut(s) 160, 468, 660, 969, 1455, 2125
Bsp119I TTCGAA 1 cut(s) 778
Bsp1286I GDGCHC 4 cut(s) 114, 624, 1541, 1965
Bsp13I TCCGGA 3 cut(s) 958, 1246, 2338
Bsp1720I GCTNAGC 2 cut(s) 69, 2528
Bsp19I CCATGG 2 cut(s) 295, 1306
BspACI CCGC 4 cut(s) 174, 380, 2487, 2607
BspANI GGCC 6 cut(s) 160, 468, 660, 969, 1455, 2125
BspCNI CTCAG 7 cut(s) 82, 262, 411, 610, 1527, 1689, 2541
BspEI TCCGGA 3 cut(s) 958, 1246, 2338
BspHI TCATGA 4 cut(s) 975, 1174, 1987, 2086
BspLI GGNNCC 3 cut(s) 1093, 2280, 2320
BspMAI CTGCAG 4 cut(s) 761, 987, 1324, 1711
BspPI GGATC 4 cut(s) 307, 2273, 2286, 2404
BspQI GCTCTTC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 778
BspT107I GGYRCC 1 cut(s) 1091
BspTI CTTAAG 1 cut(s) 1288
BsrDI GCAATG 2 cut(s) 89, 2582
BsrFI RCCGGY 1 cut(s) 1741
BsrI ACTGG 8 cut(s) 1237, 1456, 1614, 1849, 2126, 2215, 2298, 2429
BssAI RCCGGY 1 cut(s) 1741
BssECI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BssT1I CCWWGG 2 cut(s) 295, 1306
Bst2UI CCWGG 1 cut(s) 2373
Bst4CI ACNGT 7 cut(s) 140, 1010, 1190, 1571, 1861, 2393, 2536
Bst6I CTCTTC 4 cut(s) 102, 260, 1478, 1898
BstAFI CTTAAG 1 cut(s) 1288
BstAPI GCANNNNNTGC 2 cut(s) 171, 1135
BstBI TTCGAA 1 cut(s) 778
BstC8I GCNNGC 5 cut(s) 96, 971, 1756, 2317, 2565
BstDSI CCRYGG 2 cut(s) 295, 1306
BstEII GGTNACC 2 cut(s) 1565, 1648
BstF5I GGATG 4 cut(s) 131, 176, 2035, 2233
BstMAI GTCTC 3 cut(s) 1366, 1678, 1900
BstNI CCWGG 1 cut(s) 2373
BstNSI RCATGY 2 cut(s) 1120, 1411
BstPAI GACNNNNGTC 1 cut(s) 1008
BstPI GGTNACC 2 cut(s) 1565, 1648
BstSCI CCNGG 3 cut(s) 160, 944, 2371
BstSFI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
BstV1I GCAGC 8 cut(s) 52, 1693, 1739, 1763, 2031, 2088, 2579, 2631
BstV2I GAAGAC 1 cut(s) 1725
BstX2I RGATCY 4 cut(s) 1792, 2262, 2278, 2409
BstYI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 6 cut(s) 160, 468, 660, 969, 1455, 2125
BtgI CCRYGG 2 cut(s) 295, 1306
BtsCI GGATG 4 cut(s) 131, 176, 2035, 2233
BtsIMutI CAGTG 9 cut(s) 1230, 1449, 1532, 1905, 2311, 2541, 2570, 2592, 2662
Cac8I GCNNGC 5 cut(s) 96, 971, 1756, 2317, 2565
CaiI CAGNNNCTG 2 cut(s) 1715, 2372
CciI TCATGA 4 cut(s) 975, 1174, 1987, 2086
Cfr10I RCCGGY 1 cut(s) 1741
Cfr13I GGNCC 5 cut(s) 159, 466, 659, 1310, 2124
CseI GACGC 1 cut(s) 1991
Csp6I GTAC 4 cut(s) 119, 1092, 1607, 1842
CviQI GTAC 4 cut(s) 119, 1092, 1607, 1842
DraI TTTAAA 1 cut(s) 180
DraIII CACNNNGTG 1 cut(s) 2182
DriI GACNNNNNGTC 1 cut(s) 2073
EaeI YGGCCR 2 cut(s) 967, 1453
Eam1104I CTCTTC 4 cut(s) 102, 260, 1478, 1898
Eam1105I GACNNNNNGTC 1 cut(s) 2073
EarI CTCTTC 4 cut(s) 102, 260, 1478, 1898
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 4 cut(s) 112, 622, 1539, 1963
Eco130I CCWWGG 2 cut(s) 295, 1306
Eco24I GRGCYC 4 cut(s) 114, 624, 1541, 1965
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 1 cut(s) 1310
Eco53kI GAGCTC 4 cut(s) 112, 622, 1539, 1963
Eco57I CTGAAG 5 cut(s) 284, 669, 762, 1737, 2594
Eco91I GGTNACC 2 cut(s) 1565, 1648
EcoICRI GAGCTC 4 cut(s) 112, 622, 1539, 1963
EcoO109I RGGNCCY 1 cut(s) 466
EcoO65I GGTNACC 2 cut(s) 1565, 1648
EcoRI GAATTC 2 cut(s) 951, 1150
EcoRII CCWGG 1 cut(s) 2371
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 2 cut(s) 295, 1306
EcoT22I ATGCAT 2 cut(s) 580, 2350
EcoT38I GRGCYC 4 cut(s) 114, 624, 1541, 1965
ErhI CCWWGG 2 cut(s) 295, 1306
FalI AAGNNNNNCTT 2 cut(s) 2547, 2579
FaqI GGGAC 4 cut(s) 37, 1726, 1861, 2080
FauI CCCGC 2 cut(s) 2494, 2614
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
FokI GGATG 4 cut(s) 138, 183, 2042, 2240
FriOI GRGCYC 4 cut(s) 114, 624, 1541, 1965
Fsp4HI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
FspBI CTAG 4 cut(s) 470, 605, 653, 1997
GluI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 2 cut(s) 716, 870
HaeIII GGCC 6 cut(s) 160, 468, 660, 969, 1455, 2125
HapII CCGG 7 cut(s) 162, 383, 945, 959, 1247, 1742, 2339
HgaI GACGC 1 cut(s) 1991
HindIII AAGCTT 4 cut(s) 200, 1061, 1291, 1414
HpaII CCGG 7 cut(s) 162, 383, 945, 959, 1247, 1742, 2339
HphI GGTGA 8 cut(s) 535, 887, 904, 1175, 1577, 1931, 2168, 2476
Hpy166II GTNNAC 3 cut(s) 395, 1449, 1864
Hpy8I GTNNAC 3 cut(s) 395, 1449, 1864
HpyAV CCTTC 7 cut(s) 650, 1687, 1816, 2153, 2183, 2551, 2623
HpyCH4III ACNGT 7 cut(s) 140, 1010, 1190, 1571, 1861, 2393, 2536
HpyCH4IV ACGT 2 cut(s) 2379, 2614
HpySE526I ACGT 2 cut(s) 2379, 2614
Kpn2I TCCGGA 3 cut(s) 958, 1246, 2338
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 1 cut(s) 79
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 7 cut(s) 385, 889, 961, 1544, 2324, 2374, 2488
Lsp1109I GCAGC 8 cut(s) 52, 1693, 1739, 1763, 2031, 2088, 2579, 2631
LweI GCATC 3 cut(s) 161, 2056, 2218
MaeI CTAG 4 cut(s) 470, 605, 653, 1997
MaeII ACGT 2 cut(s) 2379, 2614
MaeIII GTNAC 7 cut(s) 235, 327, 785, 1565, 1577, 1648, 2362
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
MhlI GDGCHC 4 cut(s) 114, 624, 1541, 1965
MlsI TGGCCA 2 cut(s) 969, 1455
MluNI TGGCCA 2 cut(s) 969, 1455
MlyI GAGTC 4 cut(s) 59, 232, 1495, 2127
MmeI TCCRAC 6 cut(s) 1026, 1389, 1415, 2230, 2306, 2528
Mox20I TGGCCA 2 cut(s) 969, 1455
Mph1103I ATGCAT 2 cut(s) 580, 2350
MroI TCCGGA 3 cut(s) 958, 1246, 2338
MroXI GAANNNNTTC 2 cut(s) 362, 993
MscI TGGCCA 2 cut(s) 969, 1455
MslI CAYNNNNRTG 1 cut(s) 1305
Msp20I TGGCCA 2 cut(s) 969, 1455
MspA1I CMGCKG 1 cut(s) 1375
MspCI CTTAAG 1 cut(s) 1288
MspI CCGG 7 cut(s) 162, 383, 945, 959, 1247, 1742, 2339
MspR9I CCNGG 3 cut(s) 162, 946, 2373
MssI GTTTAAAC 1 cut(s) 180
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 3 cut(s) 580, 640, 807
MvaI CCWGG 1 cut(s) 2373
NciI CCSGG 2 cut(s) 162, 946
NcoI CCATGG 2 cut(s) 295, 1306
NlaIV GGNNCC 3 cut(s) 1093, 2280, 2320
NmuCI GTSAC 3 cut(s) 235, 327, 1565
NsiI ATGCAT 2 cut(s) 580, 2350
NspI RCATGY 2 cut(s) 1120, 1411
NspV TTCGAA 1 cut(s) 778
PagI TCATGA 4 cut(s) 975, 1174, 1987, 2086
PciI ACATGT 1 cut(s) 1116
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 3 cut(s) 580, 640, 807
PdmI GAANNNNTTC 2 cut(s) 362, 993
PflMI CCANNNNNTGG 2 cut(s) 61, 190
PkrI GCNGC 8 cut(s) 67, 1708, 1729, 1753, 2046, 2103, 2569, 2646
PleI GAGTC 4 cut(s) 58, 232, 1494, 2127
PmeI GTTTAAAC 1 cut(s) 180
PpsI GAGTC 4 cut(s) 58, 232, 1494, 2127
PscI ACATGT 1 cut(s) 1116
PshAI GACNNNNGTC 1 cut(s) 1008
PshBI ATTAAT 1 cut(s) 992
Psp124BI GAGCTC 4 cut(s) 114, 624, 1541, 1965
Psp6I CCWGG 1 cut(s) 2371
PspEI GGTNACC 2 cut(s) 1565, 1648
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 1 cut(s) 2371
PspN4I GGNNCC 3 cut(s) 1093, 2280, 2320
PspPI GGNCC 5 cut(s) 159, 466, 659, 1310, 2124
PstI CTGCAG 4 cut(s) 761, 987, 1324, 1711
PstNI CAGNNNCTG 2 cut(s) 1715, 2372
PsuI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
PvuII CAGCTG 1 cut(s) 1375
RsaI GTAC 4 cut(s) 120, 1093, 1608, 1843
RsaNI GTAC 4 cut(s) 119, 1092, 1607, 1842
RseI CAYNNNNRTG 1 cut(s) 1305
SacI GAGCTC 4 cut(s) 114, 624, 1541, 1965
SapI GCTCTTC 1 cut(s) 102
SatI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
Sau96I GGNCC 5 cut(s) 159, 466, 659, 1310, 2124
ScaI AGTACT 2 cut(s) 120, 1843
SchI GAGTC 4 cut(s) 59, 232, 1495, 2127
ScrFI CCNGG 3 cut(s) 162, 946, 2373
SduI GDGCHC 4 cut(s) 114, 624, 1541, 1965
SfaNI GCATC 3 cut(s) 161, 2056, 2218
SfcI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
SfuI TTCGAA 1 cut(s) 778
SinI GGWCC 1 cut(s) 1310
SmiMI CAYNNNNRTG 1 cut(s) 1305
SmlI CTYRAG 6 cut(s) 254, 550, 1202, 1288, 1400, 2405
SmoI CTYRAG 6 cut(s) 254, 550, 1202, 1288, 1400, 2405
SsiI CCGC 4 cut(s) 174, 380, 2487, 2607
SspMI CTAG 4 cut(s) 470, 605, 653, 1997
SstI GAGCTC 4 cut(s) 114, 624, 1541, 1965
StyD4I CCNGG 3 cut(s) 160, 944, 2371
StyI CCWWGG 2 cut(s) 295, 1306
TaaI ACNGT 7 cut(s) 140, 1010, 1190, 1571, 1861, 2393, 2536
TaiI ACGT 2 cut(s) 2382, 2617
TatI WGTACW 3 cut(s) 118, 1606, 1841
TscAI CASTG 8 cut(s) 1237, 1456, 1539, 1905, 2311, 2541, 2577, 2599
TseFI GTSAC 3 cut(s) 235, 327, 1565
TseI GCWGC 8 cut(s) 65, 1706, 1727, 1751, 2044, 2101, 2567, 2644
Tsp45I GTSAC 3 cut(s) 235, 327, 1565
TspRI CASTG 8 cut(s) 1237, 1456, 1539, 1905, 2311, 2541, 2577, 2599
Van91I CCANNNNNTGG 2 cut(s) 61, 190
Vha464I CTTAAG 1 cut(s) 1288
VpaK11BI GGWCC 1 cut(s) 1310
VspI ATTAAT 1 cut(s) 992
XapI RAATTY 9 cut(s) 322, 338, 459, 484, 492, 538, 922, 951, 1150
XceI RCATGY 2 cut(s) 1120, 1411
XmnI GAANNNNTTC 2 cut(s) 362, 993
XspI CTAG 4 cut(s) 470, 605, 653, 1997
ZrmI AGTACT 2 cut(s) 120, 1843
Zsp2I ATGCAT 2 cut(s) 580, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.