pycom14g06030

Leucine-rich repeat receptor-like tyrosine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010029
Physical Location & Seq
Reverse (-)
5877084 .. 5879840
2757 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g06030.1

Sequence Viewer

Length: 2667 bp
ATGGTGGTTTTGTGCTTGTTCTGTGTTCTTCTTGTTGGGTTTCTATCCGAGTCCCATCTTGTGGCTGCTCAGCTTTATGATCAATCTACATTGCTCGCCATTACCGAAGAGCTCGGAGTACTCGGATGGGGTGGCAACAGCTCAGATTACTGCTCTTGGCCTGGCGTTGGATGCGGTTTGAACCACTCTATGGTCGAAAAGCTTGATCTTTCGCACCACAGCCTCCGAGGTAATGTGACTCTTGTTTCTGAGCTCAAAGCTCTGAAGAGGCTTGACCTCTCTTACAATGATTTCCATGGATCAATTCCTTCAGCTTTTGGAAATTTGTCACAGCTTGAATTTCTTGATTTGTCTTTGAATAAGTTCGGAGGTTCAATTCCGCCGGAGCTGGGTAAACTCAGAAACCTTAGATCATTGAACCTCTCCAATAACTTTCTTGTAGGAAAAATACCTGATGAAATTCAGGGCCTAGAAAAGTTGGAGAATTTTCAAATTTCAAGTAATAAGTTGAGTGGTGTTATCCCTATGTGGGTGGGGAATTTCACCAACTTGAGAGTTTTTACTGCCTATGAGAATGCATTGGAGGGTAAAATCCCAGATAATCTAGGGTTGCTTTCTGAGCTCGAATCATTGAATCTGCATTCTAACCAGCTAGAAGGCCCAATTCCCAAAAGCATTTTTGCTTCAGGGAAGCTGGAGTTTTTGGTTCTGACCCAGAACATACTAGGTGGAGATATTCGTGAAGAAATTGGAAACTGCAGTAGCCTTTCGAGTATTCGAATTGGTAAGAGTGATCTTGTAGGTAGCATTCCTCATGCAATTGGGAATGTTAGTAGCCTTACATACTTTGAGGCTGACAATAACAATCTTTCTGGTGAGATTGCTCCAGAGTTTTCTAAGTGTTCTAATCTCACCCTCTTAAATTTAGCCTCAAATGGATTTACCGGGAGAATTCCTCCGGAGCTTGGCCAGCTCATGAATCTGCAGGAATTAATTCTCTCCGACAACAGTCTGTTTGGAGATATTCCAAAATCAATTCTCGATTGCAAGAATCTTAACAAGCTTGATCTGACCAATAATAGAATCAATGGTACCATACCAAGTGATATCTGCAACATGTCGAGATTGCAATACTTGCTGTTGGGTCAGAATTCGATACGGGGTGAAATACCTCATGAGATTGGAAACTGTGTTAAACTGCTTGAGTTGCAGATGGGAAGAAACTATTTCACTGGCAGTATCCCTCCGGAAATTGGTCATATCAAAAACTTGCAGATTGCCTTAAACTTAAGCTTCAATCATCTCCATGGACCTATTCCTGCAGATTTAGGGAAACTTGATAAGCTGGTTTCTCTTGATGTCTCCAACAATCAGCTGTCTGATGTCATTCCAACTGCACTCAAGGGCATGTTAAGCTTGATAGAGGTTAATTTCTCAAACAATCTGTTCACTGGCCCGATACCAACATTCGTGCCATTCCAAAAGAGTCCGAATGCAAGTTTTCTTGGGAATAAAGGGCTTTGTGGAGAACCACTGAGCTCCTTATGTGCAAATTCTAATGGTAGTGACCGTGCAAGTTACCATCACAGGGTTTCTTACAGGATTGTACTGGCAGTTATCGGTTCTGGTTTGGCAGTTTTTATATCGGTAACCATTGTTGTCCTGCTGTTTATGATCAGAGAGAGACAGGAGAAGGCAGAAAAAGCTGCAGGGACTGAAGACGAGGCGGCCAACAACCTACCGGAGATAGCAGCTGGCAATGTCTTTGTTGAGAATCTCAAACAAGCAATAGATCTTGATTGTGCTGTTAAAGCAACCATGAAGGATTCAAACAAGATCAGTACCGGGACTTTTAGCACAGTTTACAAGGCTGTTATGCCGTCGGGGTTGATTATATCAGTGAAGAGACTGAAATCTATGGACAGGACAATCATTCATCATCAGAACAAGATGATTCGAGAGCTCGAAAGGTTGAGCAAACTCTGTCATGACAACCTAGTGCGTCCTATCGGATTTGTTATTTACGAGGATGTTGCTCTTTTGCTGCATCATTACTTGCCCTATGGGACATTATCTCAGCTTCTTCATGAATCCACCAAGCTGCCCGAATATGAACCTGACTGGCCCAAAAGACTCTCTATTGCCATTGGAGTAGCAGAAGGATTGGCTTTCCTTCATCATGTGGCGATTATCCACCTCGATATTTCTACTGGTAATGTTCTTTTGGATGCTAACTTCAAGCCTTTGGTTGGAGAGATTGAGATCTCGAAGCTCTTGGATCCAACCAGAGGCACAGCCAGTATCAGTGCAGTTGCAGGCTCCTTTGGCTACATTCCTCCGGAATATGCATATACGATGCAAGTTACAGCGCCCGGGAACGTCTATAGCTACGGTGTTGTTTTGCTTGAGATCCTTACAACCAAATTACCAGTAGATGAAGCCTTTGATGAGGGGGTAGATTTGGTGAAGTGGGTGCATAATGCTCCGGCAAGAGGCGAAACCCCAGAGCAGATTCTCGATGCAAGGCTGAGCACAGTGTCCTTCGGTTGGAGGAAAGAAATGCTTGCAGCACTGAAGATTGCATTGCTCTGCACTGACAGCATACCCGCCAAACGTCCGAAAATGAAGAAGGTTGTGGAAATGCTGCAAGAAATAAAGAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

889

Amino Acids

97.11

Weight (kDa)

5.92

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 80 - 215 9.1e-11 Leucine-rich repeat region
LRR_14 PF23598 277 - 405 2.3e-09 Leucine-rich repeat region
LRR_8 PF13855 327 - 386 6.5e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 610 - 880 3.8e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 610 - 814 2.9e-25 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1091
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 2 cut(s) 61, 190
AccIII TCCGGA 3 cut(s) 958, 1246, 2338
AciI CCGC 4 cut(s) 174, 380, 1727, 2607
AclWI GGATC 4 cut(s) 307, 2273, 2286, 2404
AcoI YGGCCR 2 cut(s) 967, 1728
AcuI CTGAAG 5 cut(s) 284, 294, 669, 1737, 2594
AfaI GTAC 4 cut(s) 120, 1093, 1608, 1843
AflII CTTAAG 1 cut(s) 1288
AflIII ACRYGT 1 cut(s) 1116
AjnI CCWGG 1 cut(s) 160
Alw21I GWGCWC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
Alw26I GTCTC 3 cut(s) 1366, 1678, 1900
AlwI GGATC 4 cut(s) 307, 2273, 2286, 2404
AlwNI CAGNNNCTG 1 cut(s) 1715
Ama87I CYCGRG 1 cut(s) 2372
Aor13HI TCCGGA 3 cut(s) 958, 1246, 2338
AoxI GGCC 7 cut(s) 158, 466, 658, 967, 1453, 1728, 2123
ApeKI GCWGC 7 cut(s) 65, 1706, 1751, 2044, 2101, 2567, 2644
AseI ATTAAT 1 cut(s) 992
Asp700I GAANNNNTTC 2 cut(s) 362, 993
Asp718I GGTACC 1 cut(s) 1091
AspLEI GCGC 1 cut(s) 2371
AspS9I GGNCC 5 cut(s) 466, 659, 1310, 1454, 2124
AsuC2I CCSGG 4 cut(s) 946, 1846, 2373, 2374
AsuHPI GGTGA 5 cut(s) 535, 887, 904, 1175, 2476
AsuII TTCGAA 1 cut(s) 778
AvaI CYCGRG 1 cut(s) 2372
AvaII GGWCC 1 cut(s) 1310
BalI TGGCCA 1 cut(s) 969
BamHI GGATCC 1 cut(s) 2278
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 5 cut(s) 114, 255, 624, 1541, 1965
BbsI GAAGAC 1 cut(s) 1725
Bbv12I GWGCWC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
BbvI GCAGC 7 cut(s) 52, 1693, 1763, 2031, 2088, 2579, 2631
BccI CCATC 4 cut(s) 63, 120, 1207, 1590
BceAI ACGGC 1 cut(s) 1864
BcgI CGANNNNNNTGC 2 cut(s) 2015, 2049
BciT130I CCWGG 1 cut(s) 162
BciVI GTATCC 1 cut(s) 1250
BclI TGATCA 2 cut(s) 79, 1674
BcnI CCSGG 4 cut(s) 946, 1846, 2373, 2374
BcoDI GTCTC 3 cut(s) 1366, 1678, 1900
BfaI CTAG 5 cut(s) 470, 605, 653, 725, 1997
BfmI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
BfoI RGCGCY 1 cut(s) 2372
BfrI CTTAAG 1 cut(s) 1288
BfuI GTATCC 1 cut(s) 1250
BglII AGATCT 2 cut(s) 1792, 2262
BisI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
BlpI GCTNAGC 2 cut(s) 69, 2528
BlsI GCNGC 8 cut(s) 67, 1708, 1729, 1753, 2046, 2103, 2569, 2646
BmcAI AGTACT 1 cut(s) 120
Bme1390I CCNGG 5 cut(s) 162, 946, 1846, 2373, 2374
Bme18I GGWCC 1 cut(s) 1310
BmeT110I CYCGRG 1 cut(s) 2372
BmgT120I GGNCC 5 cut(s) 466, 659, 1310, 1454, 2124
BmiI GGNNCC 3 cut(s) 1093, 2280, 2320
BmrFI CCNGG 5 cut(s) 162, 946, 1846, 2373, 2374
BmsI GCATC 5 cut(s) 161, 2056, 2218, 2346, 2509
BoxI GACNNNNGTC 1 cut(s) 1008
BpiI GAAGAC 1 cut(s) 1725
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 2 cut(s) 716, 870
Bpu1102I GCTNAGC 2 cut(s) 69, 2528
Bpu14I TTCGAA 1 cut(s) 778
BpuEI CTTGAG 4 cut(s) 571, 1223, 1385, 2426
BpuMI CCSGG 4 cut(s) 946, 1846, 2373, 2374
BsaBI GATNNNNATC 1 cut(s) 2261
BsaJI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BsaWI WCCGGW 4 cut(s) 958, 1246, 1741, 2338
Bse1I ACTGG 7 cut(s) 1237, 1456, 1614, 2126, 2215, 2298, 2429
Bse3DI GCAATG 3 cut(s) 89, 1765, 2582
Bse8I GATNNNNATC 1 cut(s) 2261
BseAI TCCGGA 3 cut(s) 958, 1246, 2338
BseBI CCWGG 1 cut(s) 162
BseDI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BseGI GGATG 4 cut(s) 131, 176, 2035, 2233
BseJI GATNNNNATC 1 cut(s) 2261
BseMI GCAATG 3 cut(s) 89, 1765, 2582
BseMII CTCAG 8 cut(s) 83, 156, 240, 412, 609, 1526, 2090, 2519
BseNI ACTGG 7 cut(s) 1237, 1456, 1614, 2126, 2215, 2298, 2429
BseXI GCAGC 7 cut(s) 52, 1693, 1763, 2031, 2088, 2579, 2631
BseYI CCCAGC 1 cut(s) 388
BsgI GTGCAG 3 cut(s) 1380, 2328, 2575
BshFI GGCC 7 cut(s) 160, 468, 660, 969, 1455, 1730, 2125
BshNI GGYRCC 1 cut(s) 1091
BsiHKAI GWGCWC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
BsiHKCI CYCGRG 1 cut(s) 2372
BsiSI CCGG 9 cut(s) 383, 945, 959, 1247, 1742, 1845, 2339, 2373, 2486
BslFI GGGAC 4 cut(s) 37, 1726, 1861, 2080
BsmAI GTCTC 3 cut(s) 1366, 1678, 1900
BsmFI GGGAC 4 cut(s) 37, 1726, 1861, 2080
BsmI GAATGC 4 cut(s) 580, 640, 807, 1498
BsnI GGCC 7 cut(s) 160, 468, 660, 969, 1455, 1730, 2125
BsoBI CYCGRG 1 cut(s) 2372
Bsp119I TTCGAA 1 cut(s) 778
Bsp1286I GDGCHC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
Bsp13I TCCGGA 3 cut(s) 958, 1246, 2338
Bsp1720I GCTNAGC 2 cut(s) 69, 2528
Bsp19I CCATGG 2 cut(s) 295, 1306
BspACI CCGC 4 cut(s) 174, 380, 1727, 2607
BspANI GGCC 7 cut(s) 160, 468, 660, 969, 1455, 1730, 2125
BspCNI CTCAG 8 cut(s) 82, 155, 241, 411, 610, 1527, 2089, 2520
BspEI TCCGGA 3 cut(s) 958, 1246, 2338
BspHI TCATGA 4 cut(s) 975, 1174, 1987, 2086
BspLI GGNNCC 3 cut(s) 1093, 2280, 2320
BspMAI CTGCAG 4 cut(s) 761, 987, 1324, 1711
BspPI GGATC 4 cut(s) 307, 2273, 2286, 2404
BspQI GCTCTTC 1 cut(s) 102
BspT104I TTCGAA 1 cut(s) 778
BspT107I GGYRCC 1 cut(s) 1091
BspTI CTTAAG 1 cut(s) 1288
BsrDI GCAATG 3 cut(s) 89, 1765, 2582
BsrI ACTGG 7 cut(s) 1237, 1456, 1614, 2126, 2215, 2298, 2429
BssECI CCNNGG 4 cut(s) 226, 295, 1306, 2372
BssT1I CCWWGG 2 cut(s) 295, 1306
Bst2UI CCWGG 1 cut(s) 162
Bst4CI ACNGT 6 cut(s) 1010, 1190, 1571, 1861, 2393, 2536
Bst6I CTCTTC 3 cut(s) 102, 260, 1898
BstAFI CTTAAG 1 cut(s) 1288
BstAPI GCANNNNNTGC 1 cut(s) 1135
BstBI TTCGAA 1 cut(s) 778
BstC8I GCNNGC 5 cut(s) 96, 971, 1756, 2317, 2565
BstDSI CCRYGG 2 cut(s) 295, 1306
BstEII GGTNACC 1 cut(s) 1648
BstF5I GGATG 4 cut(s) 131, 176, 2035, 2233
BstH2I RGCGCY 1 cut(s) 2372
BstHHI GCGC 1 cut(s) 2371
BstMAI GTCTC 3 cut(s) 1366, 1678, 1900
BstNI CCWGG 1 cut(s) 162
BstNSI RCATGY 2 cut(s) 1120, 1411
BstPAI GACNNNNGTC 1 cut(s) 1008
BstPI GGTNACC 1 cut(s) 1648
BstSCI CCNGG 5 cut(s) 160, 944, 1844, 2371, 2372
BstSFI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
BstV1I GCAGC 7 cut(s) 52, 1693, 1763, 2031, 2088, 2579, 2631
BstV2I GAAGAC 1 cut(s) 1725
BstX2I RGATCY 4 cut(s) 1792, 2262, 2278, 2409
BstYI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
BsuI GTATCC 1 cut(s) 1250
BsuRI GGCC 7 cut(s) 160, 468, 660, 969, 1455, 1730, 2125
BtgI CCRYGG 2 cut(s) 295, 1306
BtsCI GGATG 4 cut(s) 131, 176, 2035, 2233
BtsIMutI CAGTG 9 cut(s) 1230, 1449, 1532, 1905, 2311, 2541, 2570, 2592, 2662
Cac8I GCNNGC 5 cut(s) 96, 971, 1756, 2317, 2565
CaiI CAGNNNCTG 1 cut(s) 1715
CciI TCATGA 4 cut(s) 975, 1174, 1987, 2086
CfoI GCGC 1 cut(s) 2371
Cfr13I GGNCC 5 cut(s) 466, 659, 1310, 1454, 2124
Cfr9I CCCGGG 1 cut(s) 2372
CseI GACGC 1 cut(s) 1991
Csp6I GTAC 4 cut(s) 119, 1092, 1607, 1842
CviQI GTAC 4 cut(s) 119, 1092, 1607, 1842
EaeI YGGCCR 2 cut(s) 967, 1728
Eam1104I CTCTTC 3 cut(s) 102, 260, 1898
EarI CTCTTC 3 cut(s) 102, 260, 1898
EciI GGCGGA 1 cut(s) 369
Ecl136II GAGCTC 5 cut(s) 112, 253, 622, 1539, 1963
Eco130I CCWWGG 2 cut(s) 295, 1306
Eco24I GRGCYC 5 cut(s) 114, 255, 624, 1541, 1965
Eco32I GATATC 1 cut(s) 1108
Eco47I GGWCC 1 cut(s) 1310
Eco53kI GAGCTC 5 cut(s) 112, 253, 622, 1539, 1963
Eco57I CTGAAG 5 cut(s) 284, 294, 669, 1737, 2594
Eco88I CYCGRG 1 cut(s) 2372
Eco91I GGTNACC 1 cut(s) 1648
EcoICRI GAGCTC 5 cut(s) 112, 253, 622, 1539, 1963
EcoO109I RGGNCCY 1 cut(s) 466
EcoO65I GGTNACC 1 cut(s) 1648
EcoRI GAATTC 2 cut(s) 951, 1150
EcoRII CCWGG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 1108
EcoT14I CCWWGG 2 cut(s) 295, 1306
EcoT22I ATGCAT 2 cut(s) 580, 2350
EcoT38I GRGCYC 5 cut(s) 114, 255, 624, 1541, 1965
ErhI CCWWGG 2 cut(s) 295, 1306
FalI AAGNNNNNCTT 2 cut(s) 2547, 2579
FaqI GGGAC 4 cut(s) 37, 1726, 1861, 2080
FauI CCCGC 1 cut(s) 2614
FbaI TGATCA 2 cut(s) 79, 1674
Fnu4HI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
FokI GGATG 4 cut(s) 138, 183, 2042, 2240
FriOI GRGCYC 5 cut(s) 114, 255, 624, 1541, 1965
Fsp4HI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
FspBI CTAG 5 cut(s) 470, 605, 653, 725, 1997
GlaI GCGC 1 cut(s) 2370
GluI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
GsaI CCCAGC 1 cut(s) 392
GsuI CTGGAG 2 cut(s) 716, 870
HaeII RGCGCY 1 cut(s) 2372
HaeIII GGCC 7 cut(s) 160, 468, 660, 969, 1455, 1730, 2125
HapII CCGG 9 cut(s) 383, 945, 959, 1247, 1742, 1845, 2339, 2373, 2486
HgaI GACGC 1 cut(s) 1991
HhaI GCGC 1 cut(s) 2371
Hin6I GCGC 1 cut(s) 2369
HinP1I GCGC 1 cut(s) 2369
HindIII AAGCTT 4 cut(s) 200, 1061, 1291, 1414
HpaII CCGG 9 cut(s) 383, 945, 959, 1247, 1742, 1845, 2339, 2373, 2486
HphI GGTGA 5 cut(s) 535, 887, 904, 1175, 2476
Hpy166II GTNNAC 3 cut(s) 395, 1449, 1864
Hpy8I GTNNAC 3 cut(s) 395, 1449, 1864
Hpy99I CGWCG 1 cut(s) 1885
HpyAV CCTTC 8 cut(s) 318, 650, 1687, 1816, 2153, 2183, 2551, 2623
HpyCH4III ACNGT 6 cut(s) 1010, 1190, 1571, 1861, 2393, 2536
HpyCH4IV ACGT 2 cut(s) 2379, 2614
HpySE526I ACGT 2 cut(s) 2379, 2614
HspAI GCGC 1 cut(s) 2369
Kpn2I TCCGGA 3 cut(s) 958, 1246, 2338
KpnI GGTACC 1 cut(s) 1095
Ksp22I TGATCA 2 cut(s) 79, 1674
LguI GCTCTTC 1 cut(s) 102
LmnI GCTCC 6 cut(s) 385, 889, 961, 1544, 2324, 2488
Lsp1109I GCAGC 7 cut(s) 52, 1693, 1763, 2031, 2088, 2579, 2631
LweI GCATC 5 cut(s) 161, 2056, 2218, 2346, 2509
MaeI CTAG 5 cut(s) 470, 605, 653, 725, 1997
MaeII ACGT 2 cut(s) 2379, 2614
MaeIII GTNAC 6 cut(s) 235, 327, 1565, 1577, 1648, 2362
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
MhlI GDGCHC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
MlsI TGGCCA 1 cut(s) 969
MluNI TGGCCA 1 cut(s) 969
MlyI GAGTC 4 cut(s) 59, 232, 1495, 2127
MmeI TCCRAC 8 cut(s) 148, 459, 1026, 1389, 1415, 2230, 2306, 2528
Mox20I TGGCCA 1 cut(s) 969
Mph1103I ATGCAT 2 cut(s) 580, 2350
MroI TCCGGA 3 cut(s) 958, 1246, 2338
MroXI GAANNNNTTC 2 cut(s) 362, 993
MscI TGGCCA 1 cut(s) 969
MseI TTAA 9 cut(s) 920, 992, 1056, 1194, 1283, 1289, 1412, 1428, 1809
MslI CAYNNNNRTG 1 cut(s) 1305
Msp20I TGGCCA 1 cut(s) 969
MspA1I CMGCKG 2 cut(s) 1375, 1754
MspCI CTTAAG 1 cut(s) 1288
MspI CCGG 9 cut(s) 383, 945, 959, 1247, 1742, 1845, 2339, 2373, 2486
MspR9I CCNGG 5 cut(s) 162, 946, 1846, 2373, 2374
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 4 cut(s) 580, 640, 807, 1498
MvaI CCWGG 1 cut(s) 162
NciI CCSGG 4 cut(s) 946, 1846, 2373, 2374
NcoI CCATGG 2 cut(s) 295, 1306
NlaIV GGNNCC 3 cut(s) 1093, 2280, 2320
NmuCI GTSAC 3 cut(s) 235, 327, 1565
NsiI ATGCAT 2 cut(s) 580, 2350
NspI RCATGY 2 cut(s) 1120, 1411
NspV TTCGAA 1 cut(s) 778
PagI TCATGA 4 cut(s) 975, 1174, 1987, 2086
PciI ACATGT 1 cut(s) 1116
PciSI GCTCTTC 1 cut(s) 102
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 4 cut(s) 580, 640, 807, 1498
PdmI GAANNNNTTC 2 cut(s) 362, 993
PflMI CCANNNNNTGG 2 cut(s) 61, 190
PkrI GCNGC 8 cut(s) 67, 1708, 1729, 1753, 2046, 2103, 2569, 2646
PleI GAGTC 4 cut(s) 58, 232, 1494, 2127
PpsI GAGTC 4 cut(s) 58, 232, 1494, 2127
PscI ACATGT 1 cut(s) 1116
PshAI GACNNNNGTC 1 cut(s) 1008
PshBI ATTAAT 1 cut(s) 992
Psp124BI GAGCTC 5 cut(s) 114, 255, 624, 1541, 1965
Psp6I CCWGG 1 cut(s) 160
PspEI GGTNACC 1 cut(s) 1648
PspFI CCCAGC 1 cut(s) 388
PspGI CCWGG 1 cut(s) 160
PspN4I GGNNCC 3 cut(s) 1093, 2280, 2320
PspPI GGNCC 5 cut(s) 466, 659, 1310, 1454, 2124
PstI CTGCAG 4 cut(s) 761, 987, 1324, 1711
PstNI CAGNNNCTG 1 cut(s) 1715
PsuI RGATCY 4 cut(s) 1792, 2262, 2278, 2409
PvuII CAGCTG 2 cut(s) 1375, 1754
RsaI GTAC 4 cut(s) 120, 1093, 1608, 1843
RsaNI GTAC 4 cut(s) 119, 1092, 1607, 1842
RseI CAYNNNNRTG 1 cut(s) 1305
SacI GAGCTC 5 cut(s) 114, 255, 624, 1541, 1965
SapI GCTCTTC 1 cut(s) 102
SaqAI TTAA 9 cut(s) 920, 992, 1056, 1194, 1283, 1289, 1412, 1428, 1809
SatI GCNGC 8 cut(s) 66, 1707, 1728, 1752, 2045, 2102, 2568, 2645
Sau96I GGNCC 5 cut(s) 466, 659, 1310, 1454, 2124
ScaI AGTACT 1 cut(s) 120
SchI GAGTC 4 cut(s) 59, 232, 1495, 2127
ScrFI CCNGG 5 cut(s) 162, 946, 1846, 2373, 2374
SduI GDGCHC 6 cut(s) 114, 255, 624, 1541, 1965, 2534
SfaNI GCATC 5 cut(s) 161, 2056, 2218, 2346, 2509
SfcI CTRYAG 5 cut(s) 757, 983, 1320, 1707, 2383
SfuI TTCGAA 1 cut(s) 778
SinI GGWCC 1 cut(s) 1310
SmaI CCCGGG 1 cut(s) 2374
SmiMI CAYNNNNRTG 1 cut(s) 1305
SmlI CTYRAG 5 cut(s) 550, 1202, 1288, 1400, 2405
SmoI CTYRAG 5 cut(s) 550, 1202, 1288, 1400, 2405
SsiI CCGC 4 cut(s) 174, 380, 1727, 2607
SspMI CTAG 5 cut(s) 470, 605, 653, 725, 1997
SstI GAGCTC 5 cut(s) 114, 255, 624, 1541, 1965
StyD4I CCNGG 5 cut(s) 160, 944, 1844, 2371, 2372
StyI CCWWGG 2 cut(s) 295, 1306
TaaI ACNGT 6 cut(s) 1010, 1190, 1571, 1861, 2393, 2536
TaiI ACGT 2 cut(s) 2382, 2617
TatI WGTACW 2 cut(s) 118, 1606
TauI GCSGC 1 cut(s) 1730
Tru1I TTAA 9 cut(s) 920, 992, 1056, 1194, 1283, 1289, 1412, 1428, 1809
Tru9I TTAA 9 cut(s) 920, 992, 1056, 1194, 1283, 1289, 1412, 1428, 1809
TscAI CASTG 8 cut(s) 1237, 1456, 1539, 1905, 2311, 2541, 2577, 2599
TseFI GTSAC 3 cut(s) 235, 327, 1565
TseI GCWGC 7 cut(s) 65, 1706, 1751, 2044, 2101, 2567, 2644
Tsp45I GTSAC 3 cut(s) 235, 327, 1565
TspMI CCCGGG 1 cut(s) 2372
TspRI CASTG 8 cut(s) 1237, 1456, 1539, 1905, 2311, 2541, 2577, 2599
Van91I CCANNNNNTGG 2 cut(s) 61, 190
Vha464I CTTAAG 1 cut(s) 1288
VpaK11BI GGWCC 1 cut(s) 1310
VspI ATTAAT 1 cut(s) 992
XceI RCATGY 2 cut(s) 1120, 1411
XmaI CCCGGG 1 cut(s) 2372
XmnI GAANNNNTTC 2 cut(s) 362, 993
XspI CTAG 5 cut(s) 470, 605, 653, 725, 1997
ZrmI AGTACT 1 cut(s) 120
Zsp2I ATGCAT 2 cut(s) 580, 2350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.