Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0005404

Physical Location & Seq

Genomic Coordinates Reverse (-)

9823197 .. 9837227

Gene Structure 14031 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1080600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGGCGACGGAGCAAAACATGAGTAGCTGGACCGATTTGCTCCACTCCTCCACCAAGCTTCTCGAACAAGCGGCGCCTTCCGCTCAGTTCCCTCCGCTACAGAGAAATTTAGATCAGTTAGAAACATTATCTAAGAAACTCAAAGCAAAAACCCTACGAAATGAGGCTCCTCAACAATCCGTTGCTGCCACAAGGCTTCTTGCACGTGAGGGACTAAATGCAGAGCAGCTTGCACGCGACCTTAAGTCCTTTGAATTGAAGACAACATTTGAAGATGTTTTTCCGTCTGAAGCAACAACTGTTGAAGAGTATCTGCAGCAGGTCCATCAAATGGCAATGGTCTCGGCTCTCCAAGAAGCTCAGAAGGATAACCTTAGAAGTTTTAATGATTACATGTTAAAGGTTTTGGAGGAGGATTGGCAAAAGGAAAAACGTGATTTCCTTCAAAGCTTAAGCCAGATTTCGACATTACCCAGGACCAACATGACTTATACTAGCAGCAGTGGTAGTCGTTCTGGTCAAATAGCATCTATAACTTCTAGCCGTCAAGTGTCATCTTCTCCATCTAGCATGGAGCTTGTGCCTTTAGCTAGCAAGCCCATCCGTGACAAGAAAGCCTCTGTCTATGCTGAAGTTGTAAAAATTCTGAACAATGCAAGGCAGCATGGATTACCATTTAAACCTGCTACAGCTTTCAAGGGTGCTTATGAGAGTTTAGGCCTTGATGTATCTGGTGGAAAATCAGTTAACATGCAGAAGATATGGCACCTTCTTCAGACATTGATGGGTGAAGATATAACCCTACCGCGTAGTGTTTCAAAAAGGATGTCATTAGTTATTGGGGCAAGGCGGCACCTAGAATGGGGGCATGAAAAATACATCATGGATACAATTCAAAGTCATCCGGGGCAGGCCGCCCTTGGAGGAGTTGTTGGAAATTTACAAAGAATCCGTGCCTTTCTTCGGATTCGTTTAAGAGATTATGGAGTTCTAGATTTTGATGCAGGCGATGCCCGCAGGCAGCCTCCTGTTGATACCACTTGGCAGCAGATCTACTTTTGCTTGAGAACTGGGTATTACGATGAGGCAAGATCTGTTGCTCTGTCATCTCGTGCTTCACACCAATTTGCTCCTCTGCTTACGGAGTGGATTAATACTGGAGGTATGGTGCCTGCGTCGATTGCAGCTTCTGCTTCAGAAGAATGTGAAAAAATGTTAAGAATGGGTGATCGTGTGGGCCGAGCTGCATATGACAAGAAAAAGTTGTTACTATATGCTCTTGTTTCTGGTTCTCGTAGGCAAATCGACCGCCTACTAAGAGATCTACCAACCCTTTTCAACACTATAGAGGATTTCTTGTGGTTCAAATTGTCTGCTGTAAGAGACTGCCCTGGTGGAGCTGCACCCATTGTTATGAATGAGAGCTTGGTACCATACACATTGGATGATTTGCAGATGTACTTAAATAAATTCGATCCATCATATTATACAAAAAATGGGAAGGACCCCCTTGTGTACCCATACGTTTTGCTTTTAAGCATCCAGTTGATACCGGGCATGGTATACTTGTTCAAAGAAACTGGAGATGAGGGATATAACATTGATGCTGCCCACATATCAATTGTGCTAGCAGACCATGGGTTCCTTTCTGAAGGTGCTGGTGCTGGACAGAAAATGGGCTTGATGGATGGTTACGCAGAGGCATCGAGCATAATCAGGCAGTATGGATCTGTATATTTACGTCTTGGTAATCTATCAGTGGCATTAGAATATTATGCACAAGCTGCTGCTGCGGTTGGTGGTGGGGAGTTGTCATGGTCTGGAAGAGGCAACGTGGACCAGCAAAGGCAGAGAAATTTGATGCTGAAGCAGCTCCTTACAGAGCTGTTATTGCGGGATGGTGGGATCTATCTACTACTTGGATCAAGAGGTGCTGGAGAAGAAGGTGAATTGGGGCGATTTTTGACTGATGTAAAAACAAGGCAACAATTTCTTCTTGAAGCTGCACACCAATGTCAGGAGGCTGGGTTGTATGAAAAGTCTATAGAAATTCAGAAACGAATAGGAGCATTCTCGATGGCATTGGATACTATTAACAAGTGCCTCTCTGAAGCAATTTGTGCCCTTTCACGTGGTAGATTGGATGGTGAGAGTCGAACTGCTGGCCTCATTCACTCTGGAAACGAGATCTTGGAAATGCATAAATACTTTCCTGATTTCAGTCCTCAAGAGAGGGAAAGTGTTTCAGAGCAGTACATTGTTTTAAGACAACTCGAGGCCGTACTGTCAATCCATAAGTTGGCAAGAGGAGGCCATTACGCTGATGCCTTAAGGGAGGTTGCCAGGCTTCAATTTCTTCCCTTAGTTCCCGGGACACCAGATGTTACCACTGATGTCTTCCAGAATTTATCTCCACATGTCCAAGCCTGTGTTCCAGACCTCTTGAAGGTCGCCCTCACTTGTCTGGACAACATGGTGGATTCTGATGGGTCTCTCCGTGCCTTGAGGGCTAAGATTGCTAGCTTCATTGCAAACAATTCCAATAGGAACTGGCCTCGTGATTTGTATGAGAAAGTTGCACGGAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016973 GO:0017038 GO:0017056 GO:0022607 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0046931 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051292 GO:0051641 GO:0051649 GO:0065003 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nic96

Protein Analysis

864

Amino Acids

96.1

Weight (kDa)

7.26

Isoelectric Point (pI)

48.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nic96	PF04097	250 - 857	8.2e-98	Nup93/Nic96

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005404)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41620 AT3G57350
fragaria_vesca	FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390
malus_domestica	MD00G1107400.v1.1 MD12G1080600.v1.1 MD14G1076700.v1.1
prunus_persica	Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1
pyrus_communis	pycom12g06710 pycom14g06500
rosa_chinensis	RchiOBHm_Chr3g0483381
rosa_laevigata	RLG00000023288
rosa_multiflora	Rmu_sc0006349.1_g000012
rosa_roxburghii	Rroxscaffold_6G00398400
rosa_rugosa	Rorug03G0205600
rosa_samantha	Rh3AG254000 Rh3CG288100 Rh3DG283900
rosa_wichuraiana	Rw0G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	310, 691
Acc65I	GGTACC	1 cut(s)	1429
AccB1I	GGYRCC	5 cut(s)	73, 765, 852, 1168, 1429
AccB7I	CCANNNNNTGG	2 cut(s)	331, 2299
AccBSI	CCGCTC	1 cut(s)	83
AccI	GTMKAC	1 cut(s)	1563
AccII	CGCG	2 cut(s)	237, 808
AclWI	GGATC	4 cut(s)	1469, 1735, 1913, 1930
AcsI	RAATTY	7 cut(s)	106, 642, 937, 1469, 1855, 2049, 2404
AcuI	CTGAAG	7 cut(s)	309, 651, 758, 1179, 1671, 1886, 2130
AcvI	CACGTG	2 cut(s)	206, 2132
AcyI	GRCGYC	1 cut(s)	74
AfaI	GTAC	5 cut(s)	1431, 1460, 1517, 2255, 2283
AfiI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 2017, 2299, 2446
AflII	CTTAAG	3 cut(s)	242, 451, 2329
AflIII	ACRYGT	2 cut(s)	393, 2416
AhdI	GACNNNNNGTC	1 cut(s)	244
AjnI	CCWGG	3 cut(s)	473, 1390, 2342
AjuI	GAANNNNNNNTTGG	4 cut(s)	1409, 1441, 2415, 2447
Alw26I	GTCTC	3 cut(s)	346, 1377, 2496
AlwI	GGATC	4 cut(s)	1469, 1735, 1913, 1930
AlwNI	CAGNNNCTG	1 cut(s)	1190
Ama87I	CYCGRG	2 cut(s)	2273, 2369
AoxI	GGCC	7 cut(s)	718, 912, 1237, 2164, 2277, 2311, 2552
ApoI	RAATTY	7 cut(s)	106, 642, 937, 1469, 1855, 2049, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	812, 844
AseI	ATTAAT	1 cut(s)	1152
Asp718I	GGTACC	1 cut(s)	1429
AspLEI	GCGC	1 cut(s)	76
AspS9I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1837
AsuC2I	CCSGG	4 cut(s)	906, 1554, 2370, 2371
AsuHPI	GGTGA	4 cut(s)	800, 1238, 1958, 2159
AsuNHI	GCTAGC	3 cut(s)	590, 1627, 2519
AvaI	CYCGRG	2 cut(s)	2273, 2369
AvaII	GGWCC	5 cut(s)	30, 322, 477, 1504, 1837
BaeGI	GKGCMC	1 cut(s)	2125
BanI	GGYRCC	5 cut(s)	73, 765, 852, 1168, 1429
BauI	CACGAG	2 cut(s)	1110, 2556
BbrPI	CACGTG	2 cut(s)	206, 2132
BbsI	GAAGAC	2 cut(s)	266, 2389
BceAI	ACGGC	2 cut(s)	528, 2264
BcgI	CGANNNNNNTGC	4 cut(s)	324, 358, 1686, 1720
BciT130I	CCWGG	3 cut(s)	475, 1392, 2344
BciVI	GTATCC	2 cut(s)	880, 2080
BcnI	CCSGG	4 cut(s)	906, 1554, 2370, 2371
BcoDI	GTCTC	3 cut(s)	346, 1377, 2496
BfaI	CTAG	8 cut(s)	495, 540, 567, 591, 857, 992, 1628, 2520
BfmI	CTRYAG	5 cut(s)	98, 314, 687, 1344, 2043
BfoI	RGCGCY	1 cut(s)	77
BfrI	CTTAAG	3 cut(s)	242, 451, 2329
BfuAI	ACCTGC	2 cut(s)	310, 691
BfuI	GTATCC	2 cut(s)	880, 2080
BglI	GCCNNNNNGGC	1 cut(s)	2507
BglII	AGATCT	4 cut(s)	1050, 1091, 1321, 2187
Bme1390I	CCNGG	7 cut(s)	475, 906, 1392, 1554, 2344, 2370, 2371
Bme18I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1837
BmeRI	GACNNNNNGTC	1 cut(s)	244
BmeT110I	CYCGRG	2 cut(s)	2273, 2369
BmgT120I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1837
BmiI	GGNNCC	8 cut(s)	75, 168, 767, 854, 1170, 1431, 1506, 1643
BmrFI	CCNGG	7 cut(s)	475, 906, 1392, 1554, 2344, 2370, 2371
BmrI	ACTGGG	1 cut(s)	1080
BmsI	GCATC	8 cut(s)	536, 991, 1000, 1548, 1594, 1712, 1851, 2314
BmtI	GCTAGC	3 cut(s)	594, 1631, 2523
BmuI	ACTGGG	1 cut(s)	1080
BpiI	GAAGAC	2 cut(s)	266, 2389
BpmI	CTGGAG	3 cut(s)	1179, 1602, 1956
BpuEI	CTTGAG	3 cut(s)	1084, 2211, 2524
BpuMI	CCSGG	4 cut(s)	906, 1554, 2370, 2371
BsaAI	YACGTR	2 cut(s)	206, 2132
BsaHI	GRCGYC	1 cut(s)	74
BsaI	GGTCTC	2 cut(s)	346, 2496
BsaJI	CCNNGG	6 cut(s)	473, 905, 919, 1390, 1636, 2369
BsaXI	ACNNNNNCTCC	6 cut(s)	915, 945, 1152, 1182, 2012, 2042
Bsc4I	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 2017, 2299, 2446
Bse1I	ACTGG	5 cut(s)	1075, 1162, 1543, 1585, 2555
Bse3DI	GCAATG	2 cut(s)	342, 2526
BseBI	CCWGG	3 cut(s)	475, 1392, 2344
BseDI	CCNNGG	6 cut(s)	473, 905, 919, 1390, 1636, 2369
BseGI	GGATG	8 cut(s)	600, 831, 901, 1450, 1539, 1693, 1903, 2149
BseLI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 2017, 2299, 2446
BseMI	GCAATG	2 cut(s)	342, 2526
BseMII	CTCAG	2 cut(s)	98, 374
BseNI	ACTGG	5 cut(s)	1075, 1162, 1543, 1585, 2555
BseRI	GAGGAG	6 cut(s)	37, 159, 425, 939, 1122, 2322
BseSI	GKGCMC	1 cut(s)	2125
BseYI	CCCAGC	1 cut(s)	2024
BsgI	GTGCAG	2 cut(s)	1386, 1989
Bsh1236I	CGCG	2 cut(s)	237, 808
Bsh1285I	CGRYCG	1 cut(s)	1309
BshFI	GGCC	7 cut(s)	720, 914, 1239, 2166, 2279, 2313, 2554
BshNI	GGYRCC	5 cut(s)	73, 765, 852, 1168, 1429
BsiEI	CGRYCG	1 cut(s)	1309
BsiHKCI	CYCGRG	2 cut(s)	2273, 2369
BsiSI	CCGG	3 cut(s)	905, 1553, 2370
BslFI	GGGAC	2 cut(s)	225, 2386
BslI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 2017, 2299, 2446
BsmAI	GTCTC	3 cut(s)	346, 1377, 2496
BsmFI	GGGAC	2 cut(s)	225, 2386
BsmI	GAATGC	1 cut(s)	2069
BsnI	GGCC	7 cut(s)	720, 914, 1239, 2166, 2279, 2313, 2554
Bso31I	GGTCTC	2 cut(s)	346, 2496
BsoBI	CYCGRG	2 cut(s)	2273, 2369
Bsp1286I	GDGCHC	1 cut(s)	2125
Bsp19I	CCATGG	1 cut(s)	1636
BspANI	GGCC	7 cut(s)	720, 914, 1239, 2166, 2279, 2313, 2554
BspCNI	CTCAG	2 cut(s)	97, 373
BspFNI	CGCG	2 cut(s)	237, 808
BspLI	GGNNCC	8 cut(s)	75, 168, 767, 854, 1170, 1431, 1506, 1643
BspMAI	CTGCAG	1 cut(s)	318
BspMI	ACCTGC	2 cut(s)	310, 691
BspOI	GCTAGC	3 cut(s)	594, 1631, 2523
BspPI	GGATC	4 cut(s)	1469, 1735, 1913, 1930
BspT107I	GGYRCC	5 cut(s)	73, 765, 852, 1168, 1429
BspTI	CTTAAG	3 cut(s)	242, 451, 2329
BspTNI	GGTCTC	2 cut(s)	346, 2496
BsrBI	CCGCTC	1 cut(s)	83
BsrDI	GCAATG	2 cut(s)	342, 2526
BsrI	ACTGG	5 cut(s)	1075, 1162, 1543, 1585, 2555
BssECI	CCNNGG	6 cut(s)	473, 905, 919, 1390, 1636, 2369
BssNAI	GTATAC	1 cut(s)	1564
BssNI	GRCGYC	1 cut(s)	74
BssSI	CACGAG	2 cut(s)	1110, 2556
BssT1I	CCWWGG	2 cut(s)	919, 1636
Bst1107I	GTATAC	1 cut(s)	1564
Bst2BI	CACGAG	2 cut(s)	1110, 2556
Bst2UI	CCWGG	3 cut(s)	475, 1392, 2344
Bst4CI	ACNGT	2 cut(s)	301, 2286
Bst6I	CTCTTC	2 cut(s)	300, 1819
BstACI	GRCGYC	1 cut(s)	74
BstAFI	CTTAAG	3 cut(s)	242, 451, 2329
BstAPI	GCANNNNNTGC	4 cut(s)	1010, 1190, 1784, 2120
BstBAI	YACGTR	2 cut(s)	206, 2132
BstDEI	CTNAG	7 cut(s)	84, 132, 360, 374, 1316, 2362, 2511
BstDSI	CCRYGG	1 cut(s)	1636
BstENI	CCTNNNNNAGG	1 cut(s)	2444
BstF5I	GGATG	8 cut(s)	600, 831, 901, 1450, 1539, 1693, 1903, 2149
BstFNI	CGCG	2 cut(s)	237, 808
BstH2I	RGCGCY	1 cut(s)	77
BstHHI	GCGC	1 cut(s)	76
BstMAI	GTCTC	3 cut(s)	346, 1377, 2496
BstMCI	CGRYCG	1 cut(s)	1309
BstNI	CCWGG	3 cut(s)	475, 1392, 2344
BstNSI	RCATGY	3 cut(s)	397, 754, 2420
BstSCI	CCNGG	7 cut(s)	473, 904, 1390, 1552, 2342, 2368, 2369
BstSFI	CTRYAG	5 cut(s)	98, 314, 687, 1344, 2043
BstSLI	GKGCMC	1 cut(s)	2125
BstUI	CGCG	2 cut(s)	237, 808
BstV2I	GAAGAC	2 cut(s)	266, 2389
BstX2I	RGATCY	6 cut(s)	1050, 1091, 1321, 1727, 1905, 2187
BstYI	RGATCY	6 cut(s)	1050, 1091, 1321, 1727, 1905, 2187
BstZ17I	GTATAC	1 cut(s)	1564
BsuI	GTATCC	2 cut(s)	880, 2080
BsuRI	GGCC	7 cut(s)	720, 914, 1239, 2166, 2279, 2313, 2554
BtgI	CCRYGG	1 cut(s)	1636
BtgZI	GCGATG	1 cut(s)	1023
BtsCI	GGATG	8 cut(s)	600, 831, 901, 1450, 1539, 1693, 1903, 2149
BtsI	GCAGTG	1 cut(s)	508
BtsIMutI	CAGTG	3 cut(s)	508, 1764, 2388
BveI	ACCTGC	2 cut(s)	310, 691
CaiI	CAGNNNCTG	1 cut(s)	1190
CfoI	GCGC	1 cut(s)	76
Cfr13I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1837
Cfr9I	CCCGGG	1 cut(s)	2369
CseI	GACGC	1 cut(s)	1164
Csp6I	GTAC	5 cut(s)	1430, 1459, 1516, 2254, 2282
CviQI	GTAC	5 cut(s)	1430, 1459, 1516, 2254, 2282
DdeI	CTNAG	7 cut(s)	84, 132, 360, 374, 1316, 2362, 2511
DinI	GGCGCC	1 cut(s)	75
DraI	TTTAAA	1 cut(s)	679
DriI	GACNNNNNGTC	1 cut(s)	244
Eam1104I	CTCTTC	2 cut(s)	300, 1819
Eam1105I	GACNNNNNGTC	1 cut(s)	244
EarI	CTCTTC	2 cut(s)	300, 1819
Eco130I	CCWWGG	2 cut(s)	919, 1636
Eco147I	AGGCCT	1 cut(s)	720
Eco31I	GGTCTC	2 cut(s)	346, 2496
Eco47I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1837
Eco57I	CTGAAG	7 cut(s)	309, 651, 758, 1179, 1671, 1886, 2130
Eco72I	CACGTG	2 cut(s)	206, 2132
Eco88I	CYCGRG	2 cut(s)	2273, 2369
EcoNI	CCTNNNNNAGG	1 cut(s)	2444
EcoO109I	RGGNCCY	1 cut(s)	1504
EcoRII	CCWGG	3 cut(s)	473, 1390, 2342
EcoT14I	CCWWGG	2 cut(s)	919, 1636
EcoT22I	ATGCAT	1 cut(s)	2202
EgeI	GGCGCC	1 cut(s)	75
EheI	GGCGCC	1 cut(s)	75
ErhI	CCWWGG	2 cut(s)	919, 1636
FaqI	GGGAC	2 cut(s)	225, 2386
FauI	CCCGC	2 cut(s)	1022, 1887
FauNDI	CATATG	1 cut(s)	1249
FblI	GTMKAC	1 cut(s)	1563
FokI	GGATG	8 cut(s)	587, 838, 888, 1457, 1526, 1700, 1910, 2156
FspBI	CTAG	8 cut(s)	495, 540, 567, 591, 857, 992, 1628, 2520
GlaI	GCGC	1 cut(s)	75
GsaI	CCCAGC	1 cut(s)	2028
GsuI	CTGGAG	3 cut(s)	1179, 1602, 1956
HaeII	RGCGCY	1 cut(s)	77
HaeIII	GGCC	7 cut(s)	720, 914, 1239, 2166, 2279, 2313, 2554
HapII	CCGG	3 cut(s)	905, 1553, 2370
HgaI	GACGC	1 cut(s)	1164
HhaI	GCGC	1 cut(s)	76
Hin1I	GRCGYC	1 cut(s)	74
Hin6I	GCGC	1 cut(s)	74
HinP1I	GCGC	1 cut(s)	74
HincII	GTYRAC	1 cut(s)	748
HindII	GTYRAC	1 cut(s)	748
HindIII	AAGCTT	2 cut(s)	56, 448
HinfI	GANTC	4 cut(s)	948, 967, 2152, 2480
HpaI	GTTAAC	1 cut(s)	748
HpaII	CCGG	3 cut(s)	905, 1553, 2370
HphI	GGTGA	4 cut(s)	800, 1238, 1958, 2159
Hpy166II	GTNNAC	4 cut(s)	748, 1516, 1564, 1837
Hpy8I	GTNNAC	4 cut(s)	748, 1516, 1564, 1837
Hpy99I	CGWCG	2 cut(s)	10, 1180
HpyAV	CCTTC	8 cut(s)	87, 358, 452, 779, 1495, 1646, 1937, 2440
HpyCH4III	ACNGT	2 cut(s)	301, 2286
HpyCH4IV	ACGT	6 cut(s)	205, 433, 1524, 1741, 1833, 2131
HpyF3I	CTNAG	7 cut(s)	84, 132, 360, 374, 1316, 2362, 2511
HpySE526I	ACGT	6 cut(s)	205, 433, 1524, 1741, 1833, 2131
Hsp92I	GRCGYC	1 cut(s)	74
HspAI	GCGC	1 cut(s)	74
KasI	GGCGCC	1 cut(s)	73
KpnI	GGTACC	1 cut(s)	1433
KspAI	GTTAAC	1 cut(s)	748
LmnI	GCTCC	9 cut(s)	10, 45, 172, 574, 1135, 1397, 1878, 2066, 2583
LweI	GCATC	8 cut(s)	536, 991, 1000, 1548, 1594, 1712, 1851, 2314
MaeI	CTAG	8 cut(s)	495, 540, 567, 591, 857, 992, 1628, 2520
MaeII	ACGT	6 cut(s)	205, 433, 1524, 1741, 1833, 2131
MaeIII	GTNAC	4 cut(s)	605, 1266, 1691, 2383
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	1 cut(s)	1620
MflI	RGATCY	6 cut(s)	1050, 1091, 1321, 1727, 1905, 2187
MhlI	GDGCHC	1 cut(s)	2125
Mly113I	GGCGCC	1 cut(s)	74
MlyI	GAGTC	1 cut(s)	2161
MmeI	TCCRAC	1 cut(s)	913
Mph1103I	ATGCAT	1 cut(s)	2202
MslI	CAYNNNNRTG	2 cut(s)	1412, 2011
MspCI	CTTAAG	3 cut(s)	242, 451, 2329
MspI	CCGG	3 cut(s)	905, 1553, 2370
MspR9I	CCNGG	7 cut(s)	475, 906, 1392, 1554, 2344, 2370, 2371
MunI	CAATTG	1 cut(s)	1620
Mva1269I	GAATGC	1 cut(s)	2069
MvaI	CCWGG	3 cut(s)	475, 1392, 2344
MvnI	CGCG	2 cut(s)	237, 808
NarI	GGCGCC	1 cut(s)	74
NciI	CCSGG	4 cut(s)	906, 1554, 2370, 2371
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	1 cut(s)	1249
NheI	GCTAGC	3 cut(s)	590, 1627, 2519
NlaIV	GGNNCC	8 cut(s)	75, 168, 767, 854, 1170, 1431, 1506, 1643
NmeAIII	GCCGAG	2 cut(s)	323, 1265
NmuCI	GTSAC	1 cut(s)	605
NsiI	ATGCAT	1 cut(s)	2202
NspI	RCATGY	3 cut(s)	397, 754, 2420
PaeR7I	CTCGAG	1 cut(s)	2273
PceI	AGGCCT	1 cut(s)	720
PciI	ACATGT	2 cut(s)	393, 2416
PcsI	WCGNNNNNNNCGW	1 cut(s)	1237
PctI	GAATGC	1 cut(s)	2069
PfeI	GAWTC	3 cut(s)	948, 967, 2480
PflMI	CCANNNNNTGG	2 cut(s)	331, 2299
PleI	GAGTC	1 cut(s)	2160
PluTI	GGCGCC	1 cut(s)	77
PmaCI	CACGTG	2 cut(s)	206, 2132
PmlI	CACGTG	2 cut(s)	206, 2132
PpsI	GAGTC	1 cut(s)	2160
Ppu21I	YACGTR	2 cut(s)	206, 2132
PpuMI	RGGWCCY	1 cut(s)	1504
PscI	ACATGT	2 cut(s)	393, 2416
PshBI	ATTAAT	1 cut(s)	1152
Psp5II	RGGWCCY	1 cut(s)	1504
Psp6I	CCWGG	3 cut(s)	473, 1390, 2342
PspCI	CACGTG	2 cut(s)	206, 2132
PspFI	CCCAGC	1 cut(s)	2024
PspGI	CCWGG	3 cut(s)	473, 1390, 2342
PspN4I	GGNNCC	8 cut(s)	75, 168, 767, 854, 1170, 1431, 1506, 1643
PspPI	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1837
PspPPI	RGGWCCY	1 cut(s)	1504
PspXI	VCTCGAGB	1 cut(s)	2273
PstI	CTGCAG	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	1190
PsuI	RGATCY	6 cut(s)	1050, 1091, 1321, 1727, 1905, 2187
RsaI	GTAC	5 cut(s)	1431, 1460, 1517, 2255, 2283
RsaNI	GTAC	5 cut(s)	1430, 1459, 1516, 2254, 2282
RseI	CAYNNNNRTG	2 cut(s)	1412, 2011
Sau96I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1837
SchI	GAGTC	1 cut(s)	2161
ScrFI	CCNGG	7 cut(s)	475, 906, 1392, 1554, 2344, 2370, 2371
SduI	GDGCHC	1 cut(s)	2125
SfaNI	GCATC	8 cut(s)	536, 991, 1000, 1548, 1594, 1712, 1851, 2314
SfcI	CTRYAG	5 cut(s)	98, 314, 687, 1344, 2043
SfoI	GGCGCC	1 cut(s)	75
Sfr274I	CTCGAG	1 cut(s)	2273
SinI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1837
SlaI	CTCGAG	1 cut(s)	2273
SmaI	CCCGGG	1 cut(s)	2371
SmiMI	CAYNNNNRTG	2 cut(s)	1412, 2011
SmlI	CTYRAG	7 cut(s)	242, 451, 1063, 2226, 2273, 2329, 2503
SmoI	CTYRAG	7 cut(s)	242, 451, 1063, 2226, 2273, 2329, 2503
SseBI	AGGCCT	1 cut(s)	720
SspDI	GGCGCC	1 cut(s)	73
SspI	AATATT	1 cut(s)	1772
SspMI	CTAG	8 cut(s)	495, 540, 567, 591, 857, 992, 1628, 2520
StuI	AGGCCT	1 cut(s)	720
StyD4I	CCNGG	7 cut(s)	473, 904, 1390, 1552, 2342, 2368, 2369
StyI	CCWWGG	2 cut(s)	919, 1636
TaaI	ACNGT	2 cut(s)	301, 2286
TaiI	ACGT	6 cut(s)	208, 436, 1527, 1744, 1836, 2134
TaqI	TCGA	9 cut(s)	63, 464, 1178, 1305, 1473, 1706, 2075, 2155, 2274
TaqII	GACCGA	1 cut(s)	47
TatI	WGTACW	2 cut(s)	1458, 2253
TauI	GCSGC	3 cut(s)	74, 853, 917
TfiI	GAWTC	3 cut(s)	948, 967, 2480
TscAI	CASTG	3 cut(s)	508, 1764, 2395
TseFI	GTSAC	1 cut(s)	605
Tsp45I	GTSAC	1 cut(s)	605
TspDTI	ATGAA	4 cut(s)	885, 1430, 2049, 2515
TspGWI	ACGGA	7 cut(s)	23, 169, 273, 593, 941, 1157, 2486
TspMI	CCCGGG	1 cut(s)	2369
TspRI	CASTG	3 cut(s)	508, 1764, 2395
Van91I	CCANNNNNTGG	2 cut(s)	331, 2299
Vha464I	CTTAAG	3 cut(s)	242, 451, 2329
VpaK11BI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1837
VspI	ATTAAT	1 cut(s)	1152
XagI	CCTNNNNNAGG	1 cut(s)	2444
XapI	RAATTY	7 cut(s)	106, 642, 937, 1469, 1855, 2049, 2404
XbaI	TCTAGA	1 cut(s)	991
XceI	RCATGY	3 cut(s)	397, 754, 2420
XhoI	CTCGAG	1 cut(s)	2273
XmaI	CCCGGG	1 cut(s)	2369
XmiI	GTMKAC	1 cut(s)	1563
XspI	CTAG	8 cut(s)	495, 540, 567, 591, 857, 992, 1628, 2520
Zsp2I	ATGCAT	1 cut(s)	2202

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.