Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0005404

Physical Location & Seq

Genomic Coordinates Forward (+)

21196577 .. 21204276

Gene Structure 7700 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000023288

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGGCGACCGATCAAAATATGAGCAGCTGGACCGATCTCCTCCACTCCTCCACCAAGCTTCTCGAACAAGCCGCTCCCTCCGCTCAGTTTCCCCCTCTTCAGAGAAATTTGGACCAGTTGGAAACATTATCTAAGAAGCTCAAGGCGAAAACTCTACGAAATGAGGCTCCTCAACAATCCATTGCTGCCACGAGACTTCTTGCTCGTGAGGGTCTAAACGCAGAGCAGCTTGCACGGGACCTTAAGTCTTTTGAATTGAAGACAACATTTGAGGATGTTTTTCCTGCTGAAGCAACGAGCATTGAAGACTATCTGCAGCAGGTCCATGAAATGGCAATGGTCTCAGCTGTTCAAGAAGCTCAGAAGGATAACCTTAGAAGCTTTAATGATCATATGTTAAGAGTTTTGGAGGAGGATTGGCAGATGGAAAAACGTGATTTCCTTCAAAGCTTAAGCCAGATTTCAACATTACCCAGGACTAATGTGACTCATACTGTCAGCGGTGGTAATCGTCCTGGTCAAGTAGCATCCATTGCTTCCAGCCCTCATGTTTCATCTGGTCCATCTAGCATGGAGCTTGTACCCCTAGATAGCAAGCCCATCCTTGAGAGAAAAGCCTCTGTTTATGCGGAAGTTGTAAAGAATCTCAACAATGCAAGGCAGCGCGGTTTACCTTTTAAGCCCGCTACAGCTTTCAAGGGTGCGTATGAAAGTTTGGGCCTTCATGCATCTGGAGGAAAATCAGTGAACATGCGGAAGATATGGCACCTTCTTCAGACGTTGCTGAGTGAAGATATAACCATACAACGAAGTGTTTCAAAAAAGATGTCATTAGTTATTGGTGCAAGGCGCCACCTGGAATGGGGGCATGAAAAATACATCATGGATACAATTCAAAATCACCCTGCACAGGCTGCTCTTGGAGGGGTTGTTGGAAATTTACAAAGAATTCGAGCCTTCCTTCGGATTCGTTTAAGAGATTATGGAGTTCTAGATTTTGATGCAGTCGATTCGCGGAGGCAGCCTCCTGTTGATACCACTTGGCAGCAGATCTACTTTTGCATGAGAACTGGCTATTATGATGAAGCAAGGTCCGTTGCTTTGTCATCTCGTGCTTCAAACCACTTTGCTCCTCTGCTCACTGAGTGGATTAATACTGGAGGTATGGTGCCTGCCGAGATTGCAACTGCGGCATCTGAGGAATGTGAAAAAATTCTAAGAATTGGTGATCGTGCTGGTCGACCTGCATATGACAAGAAAAAGTTGTTACTGCATGCTCTCATATCTGGTTCTCGTAGGCAAATCGACCGTCTGCTACGTGATCTACCAACTCTTTTCAATACTATAGAGGATTTCTTGTGGTTCAAATTGTCAGCTGTAAGAGACTGCCCGGTTGGAGCTGCATCCATTGTGATGAATGAGAGCTTGGTACCATACACGTTGGAAGATTTGCAGGCTTACTTAAATAAATTTGACCCATCATATTACACAAAAAACGGAAAGGACCCTCTCGTATATCCATACATTCTGCTTTTAAGCATCCAGTTGATACCAGCTGTCGTATACTTGTCTCAAGAATCTGGAGACGAGGGATACAACATTGATGCTGCCCACATATCAATTGTGTTAGCAGACCATGGGGTCCTTTCTGAAGGTGCTGGAGCTGGACAAAAGATGGGAGTGATGGATGCTTATGCAGAGGCATCTAGCATCATCAGGCAGTATGGATCTGCATATTTACGTGTTGGTAATCTATCAATGGCATTAGAATATTATGCACAGGCTGCTGCTGCAGTTGGTGGTGGCCATTTGTCATGGTCTGGAAGAGGTAATGTGGATCAGCAAAGGCAAAGAAATTTGATGCTGAAGCAACTACTTACAGAGCTATTGTTGCGGGATGGTGGGGTGTATCTTTTACTTGGTTCAAGAGGTGCTGGCGAAGAAGGTGAACTGAGACGATTTTTGCTTGATGTAAAAGCAAGACACCAATTTCTGCTTGAAGCCGCTCACCATTGTCAGGAAACTGGGTTGTATGAAAAATCCATAGAAATTCAGAAACGAATAGGGGCATTCTCAATGGCATTGGATACCATTAACAAGTGCCTCTCAGAAGCAATTTGTGCCTTGTCACGTGGCCGATCGGATGGGGAGAGTCGAACTGCTGGTCTCATTCATTCCGGAAATGAGATCTTGGAGATGGACAAATATTCATCTGAAGTCAGTCCTCAAGAGAGGGAGAGTGTTTTAGAGCAGCAGACTGTTTTAAGACAACTTGAGGCCATACTGTCAATACATAAGTTGGCAAGACTAGGTCATTATGTTGATGCTTTGAGGGAGGTTGCCAGACTTCAATTCCTTCCTTTAGATCCTCGAACACCAGATACTACCACTGATGTGTTCAAGAACTTAAGTCCCCATGTCCAAGCTTGTGTTCCGGACCTCTTGAAAGTTGCACTGACTTGTCTGGACAACATGGTGGATTCTGATGGATCACTCCGAGCCTTGAGGGCTAAGATTGCTAGCTTCATTGCAAATAATTCCAGTAGAAATTGGCCACGTGATTTGTACGAAAAACTTGCCCGGAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016973 GO:0017038 GO:0017056 GO:0022607 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0046931 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051292 GO:0051641 GO:0051649 GO:0065003 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nic96

Protein Analysis

864

Amino Acids

96.11

Weight (kDa)

6.91

Isoelectric Point (pI)

48.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nic96	PF04097	250 - 857	2e-101	Nup93/Nic96

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005404)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41620 AT3G57350
fragaria_vesca	FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390
malus_domestica	MD00G1107400.v1.1 MD12G1080600.v1.1 MD14G1076700.v1.1
prunus_persica	Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1
pyrus_communis	pycom12g06710 pycom14g06500
rosa_chinensis	RchiOBHm_Chr3g0483381
rosa_laevigata	RLG00000023288
rosa_multiflora	Rmu_sc0006349.1_g000012
rosa_roxburghii	Rroxscaffold_6G00398400
rosa_rugosa	Rorug03G0205600
rosa_samantha	Rh3AG254000 Rh3CG288100 Rh3DG283900
rosa_wichuraiana	Rw0G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1640
Acc36I	ACCTGC	2 cut(s)	310, 1252
Acc65I	GGTACC	1 cut(s)	1429
AccB1I	GGYRCC	4 cut(s)	765, 849, 1168, 1429
AccB7I	CCANNNNNTGG	1 cut(s)	331
AccBSI	CCGCTC	3 cut(s)	74, 83, 2006
AccI	GTMKAC	2 cut(s)	1240, 1563
AccII	CGCG	2 cut(s)	666, 1015
AccIII	TCCGGA	2 cut(s)	2177, 2434
AclWI	GGATC	4 cut(s)	1735, 1845, 2360, 2497
AcoI	YGGCCR	3 cut(s)	1804, 2134, 2552
AcsI	RAATTY	7 cut(s)	106, 937, 948, 1212, 1469, 1855, 2049
AcuI	CTGAAG	6 cut(s)	83, 309, 758, 1671, 1886, 2235
AcvI	CACGTG	2 cut(s)	2132, 2558
AcyI	GRCGYC	1 cut(s)	850
AdeI	CACNNNGTG	1 cut(s)	1146
AfaI	GTAC	3 cut(s)	582, 1431, 2567
AfiI	CCNNNNNNNGG	5 cut(s)	331, 862, 910, 963, 2017
AflII	CTTAAG	3 cut(s)	242, 451, 2407
AflIII	ACRYGT	2 cut(s)	1437, 1741
AhdI	GACNNNNNGTC	1 cut(s)	244
AjnI	CCWGG	3 cut(s)	473, 514, 855
AjuI	GAANNNNNNNTTGG	4 cut(s)	1409, 1441, 2415, 2447
AleI	CACNNNNGTG	1 cut(s)	2393
Alw26I	GTCTC	7 cut(s)	187, 346, 1377, 1575, 1578, 1948, 2171
AlwI	GGATC	4 cut(s)	1735, 1845, 2360, 2497
Aor13HI	TCCGGA	2 cut(s)	2177, 2434
AoxI	GGCC	5 cut(s)	718, 1804, 2134, 2277, 2552
ApoI	RAATTY	7 cut(s)	106, 937, 948, 1212, 1469, 1855, 2049
ArsI	GACNNNNNNTTYG	6 cut(s)	812, 844, 1294, 1326, 2149, 2181
AseI	ATTAAT	1 cut(s)	1152
Asp700I	GAANNNNTTC	2 cut(s)	814, 1212
Asp718I	GGTACC	1 cut(s)	1429
AspLEI	GCGC	2 cut(s)	666, 852
AsuC2I	CCSGG	2 cut(s)	1391, 2581
AsuHPI	GGTGA	4 cut(s)	893, 1238, 1958, 2000
AsuNHI	GCTAGC	1 cut(s)	2519
AvaII	GGWCC	9 cut(s)	30, 112, 238, 322, 560, 1092, 1504, 1642, 2437
BalI	TGGCCA	2 cut(s)	1806, 2554
BanI	GGYRCC	4 cut(s)	765, 849, 1168, 1429
BauI	CACGAG	3 cut(s)	190, 204, 1110
BbrPI	CACGTG	2 cut(s)	2132, 2558
BbsI	GAAGAC	2 cut(s)	266, 312
BcgI	CGANNNNNNTGC	4 cut(s)	993, 1027, 2558, 2592
BciT130I	CCWGG	3 cut(s)	475, 516, 857
BciVI	GTATCC	3 cut(s)	880, 1586, 2080
BclI	TGATCA	1 cut(s)	388
BcnI	CCSGG	2 cut(s)	1391, 2581
BcoDI	GTCTC	7 cut(s)	187, 346, 1377, 1575, 1578, 1948, 2171
BfaI	CTAG	6 cut(s)	567, 587, 992, 1707, 2309, 2520
BfmI	CTRYAG	4 cut(s)	314, 687, 1344, 1791
BfoI	RGCGCY	1 cut(s)	853
BfrI	CTTAAG	3 cut(s)	242, 451, 2407
BfuAI	ACCTGC	2 cut(s)	310, 1252
BfuI	GTATCC	3 cut(s)	880, 1586, 2080
BglI	GCCNNNNNGGC	1 cut(s)	2507
BglII	AGATCT	2 cut(s)	1050, 2187
Bme1390I	CCNGG	5 cut(s)	475, 516, 857, 1391, 2581
Bme18I	GGWCC	9 cut(s)	30, 112, 238, 322, 560, 1092, 1504, 1642, 2437
BmeRI	GACNNNNNGTC	1 cut(s)	244
BmiI	GGNNCC	8 cut(s)	168, 239, 767, 851, 1170, 1431, 1506, 1643
BmrFI	CCNGG	5 cut(s)	475, 516, 857, 1391, 2581
BmrI	ACTGGG	1 cut(s)	2034
BmtI	GCTAGC	1 cut(s)	2523
BmuI	ACTGGG	1 cut(s)	2034
BpiI	GAAGAC	2 cut(s)	266, 312
BplI	GAGNNNNNCTC	2 cut(s)	1009, 1041
BpmI	CTGGAG	4 cut(s)	753, 1179, 1602, 1680
BpuEI	CTTGAG	6 cut(s)	125, 626, 1557, 2211, 2294, 2524
BpuMI	CCSGG	2 cut(s)	1391, 2581
BsaAI	YACGTR	4 cut(s)	1319, 1742, 2132, 2558
BsaHI	GRCGYC	1 cut(s)	850
BsaI	GGTCTC	2 cut(s)	346, 2171
BsaJI	CCNNGG	2 cut(s)	473, 1636
BsaWI	WCCGGW	2 cut(s)	2177, 2434
BsaXI	ACNNNNNCTCC	4 cut(s)	915, 945, 1152, 1182
Bsc4I	CCNNNNNNNGG	5 cut(s)	331, 862, 910, 963, 2017
Bse1I	ACTGG	6 cut(s)	115, 1075, 1162, 1543, 2029, 2541
Bse3DI	GCAATG	4 cut(s)	180, 342, 531, 2526
BseAI	TCCGGA	2 cut(s)	2177, 2434
BseBI	CCWGG	3 cut(s)	475, 516, 857
BseDI	CCNNGG	2 cut(s)	473, 1636
BseGI	GGATG	8 cut(s)	280, 527, 600, 1403, 1539, 1693, 1903, 2149
BseLI	CCNNNNNNNGG	5 cut(s)	331, 862, 910, 963, 2017
BseMI	GCAATG	4 cut(s)	180, 342, 531, 2526
BseMII	CTCAG	8 cut(s)	98, 357, 374, 776, 1134, 1188, 1943, 2121
BseNI	ACTGG	6 cut(s)	115, 1075, 1162, 1543, 2029, 2541
BseRI	GAGGAG	5 cut(s)	29, 37, 159, 425, 1122
BsgI	GTGCAG	1 cut(s)	891
Bsh1236I	CGCG	2 cut(s)	666, 1015
Bsh1285I	CGRYCG	3 cut(s)	9, 1309, 2141
BshFI	GGCC	5 cut(s)	720, 1806, 2136, 2279, 2554
BshNI	GGYRCC	4 cut(s)	765, 849, 1168, 1429
BsiEI	CGRYCG	3 cut(s)	9, 1309, 2141
BsiSI	CCGG	4 cut(s)	1391, 2178, 2435, 2581
BslFI	GGGAC	2 cut(s)	251, 2397
BslI	CCNNNNNNNGG	5 cut(s)	331, 862, 910, 963, 2017
BsmAI	GTCTC	7 cut(s)	187, 346, 1377, 1575, 1578, 1948, 2171
BsmBI	CGTCTC	2 cut(s)	1578, 1948
BsmFI	GGGAC	2 cut(s)	251, 2397
BsmI	GAATGC	1 cut(s)	2069
BsnI	GGCC	5 cut(s)	720, 1806, 2136, 2279, 2554
Bso31I	GGTCTC	2 cut(s)	346, 2171
Bsp13I	TCCGGA	2 cut(s)	2177, 2434
Bsp19I	CCATGG	1 cut(s)	1636
BspANI	GGCC	5 cut(s)	720, 1806, 2136, 2279, 2554
BspCNI	CTCAG	8 cut(s)	97, 356, 373, 777, 1135, 1189, 1944, 2120
BspEI	TCCGGA	2 cut(s)	2177, 2434
BspFNI	CGCG	2 cut(s)	666, 1015
BspLI	GGNNCC	8 cut(s)	168, 239, 767, 851, 1170, 1431, 1506, 1643
BspMAI	CTGCAG	2 cut(s)	318, 1795
BspMI	ACCTGC	2 cut(s)	310, 1252
BspOI	GCTAGC	1 cut(s)	2523
BspPI	GGATC	4 cut(s)	1735, 1845, 2360, 2497
BspT107I	GGYRCC	4 cut(s)	765, 849, 1168, 1429
BspTI	CTTAAG	3 cut(s)	242, 451, 2407
BspTNI	GGTCTC	2 cut(s)	346, 2171
BsrBI	CCGCTC	3 cut(s)	74, 83, 2006
BsrDI	GCAATG	4 cut(s)	180, 342, 531, 2526
BsrI	ACTGG	6 cut(s)	115, 1075, 1162, 1543, 2029, 2541
BssECI	CCNNGG	2 cut(s)	473, 1636
BssNAI	GTATAC	1 cut(s)	1564
BssNI	GRCGYC	1 cut(s)	850
BssSI	CACGAG	3 cut(s)	190, 204, 1110
BssT1I	CCWWGG	1 cut(s)	1636
Bst1107I	GTATAC	1 cut(s)	1564
Bst2BI	CACGAG	3 cut(s)	190, 204, 1110
Bst2UI	CCWGG	3 cut(s)	475, 516, 857
Bst4CI	ACNGT	4 cut(s)	496, 1310, 2260, 2286
Bst6I	CTCTTC	2 cut(s)	102, 1819
BstACI	GRCGYC	1 cut(s)	850
BstAFI	CTTAAG	3 cut(s)	242, 451, 2407
BstAPI	GCANNNNNTGC	4 cut(s)	533, 914, 1784, 2120
BstBAI	YACGTR	4 cut(s)	1319, 1742, 2132, 2558
BstC8I	GCNNGC	8 cut(s)	231, 596, 684, 1173, 1275, 1455, 1936, 2521
BstDSI	CCRYGG	1 cut(s)	1636
BstF5I	GGATG	8 cut(s)	280, 527, 600, 1403, 1539, 1693, 1903, 2149
BstFNI	CGCG	2 cut(s)	666, 1015
BstH2I	RGCGCY	1 cut(s)	853
BstHHI	GCGC	2 cut(s)	666, 852
BstMAI	GTCTC	7 cut(s)	187, 346, 1377, 1575, 1578, 1948, 2171
BstMCI	CGRYCG	3 cut(s)	9, 1309, 2141
BstNI	CCWGG	3 cut(s)	475, 516, 857
BstNSI	RCATGY	2 cut(s)	754, 1277
BstSCI	CCNGG	5 cut(s)	473, 514, 855, 1389, 2579
BstSFI	CTRYAG	4 cut(s)	314, 687, 1344, 1791
BstUI	CGCG	2 cut(s)	666, 1015
BstV2I	GAAGAC	2 cut(s)	266, 312
BstX2I	RGATCY	4 cut(s)	1050, 1727, 2187, 2365
BstYI	RGATCY	4 cut(s)	1050, 1727, 2187, 2365
BstZ17I	GTATAC	1 cut(s)	1564
BsuI	GTATCC	3 cut(s)	880, 1586, 2080
BsuRI	GGCC	5 cut(s)	720, 1806, 2136, 2279, 2554
BtgI	CCRYGG	1 cut(s)	1636
BtsCI	GGATG	8 cut(s)	280, 527, 600, 1403, 1539, 1693, 1903, 2149
BtsIMutI	CAGTG	4 cut(s)	750, 1140, 2388, 2453
BveI	ACCTGC	2 cut(s)	310, 1252
Cac8I	GCNNGC	8 cut(s)	231, 596, 684, 1173, 1275, 1455, 1936, 2521
CfoI	GCGC	2 cut(s)	666, 852
Csp6I	GTAC	3 cut(s)	581, 1430, 2566
CspCI	CAANNNNNGTGG	2 cut(s)	2544, 2579
CviQI	GTAC	3 cut(s)	581, 1430, 2566
DinI	GGCGCC	1 cut(s)	851
DraIII	CACNNNGTG	1 cut(s)	1146
DrdI	GACNNNNNNGTC	1 cut(s)	1640
DriI	GACNNNNNGTC	1 cut(s)	244
DseDI	GACNNNNNNGTC	1 cut(s)	1640
EaeI	YGGCCR	3 cut(s)	1804, 2134, 2552
Eam1104I	CTCTTC	2 cut(s)	102, 1819
Eam1105I	GACNNNNNGTC	1 cut(s)	244
EarI	CTCTTC	2 cut(s)	102, 1819
Eco130I	CCWWGG	1 cut(s)	1636
Eco31I	GGTCTC	2 cut(s)	346, 2171
Eco47I	GGWCC	9 cut(s)	30, 112, 238, 322, 560, 1092, 1504, 1642, 2437
Eco57I	CTGAAG	6 cut(s)	83, 309, 758, 1671, 1886, 2235
Eco72I	CACGTG	2 cut(s)	2132, 2558
EcoO109I	RGGNCCY	3 cut(s)	238, 1504, 1642
EcoRI	GAATTC	1 cut(s)	948
EcoRII	CCWGG	3 cut(s)	473, 514, 855
EcoT14I	CCWWGG	1 cut(s)	1636
EcoT22I	ATGCAT	1 cut(s)	730
EgeI	GGCGCC	1 cut(s)	851
EheI	GGCGCC	1 cut(s)	851
ErhI	CCWWGG	1 cut(s)	1636
Esp3I	CGTCTC	2 cut(s)	1578, 1948
FaqI	GGGAC	2 cut(s)	251, 2397
FauI	CCCGC	2 cut(s)	691, 1887
FauNDI	CATATG	2 cut(s)	393, 1249
FbaI	TGATCA	1 cut(s)	388
FblI	GTMKAC	2 cut(s)	1240, 1563
FokI	GGATG	8 cut(s)	287, 514, 587, 1390, 1526, 1700, 1910, 2156
FspBI	CTAG	6 cut(s)	567, 587, 992, 1707, 2309, 2520
GlaI	GCGC	2 cut(s)	665, 851
GsuI	CTGGAG	4 cut(s)	753, 1179, 1602, 1680
HaeII	RGCGCY	1 cut(s)	853
HaeIII	GGCC	5 cut(s)	720, 1806, 2136, 2279, 2554
HapII	CCGG	4 cut(s)	1391, 2178, 2435, 2581
HhaI	GCGC	2 cut(s)	666, 852
Hin1I	GRCGYC	1 cut(s)	850
Hin6I	GCGC	2 cut(s)	664, 850
HinP1I	GCGC	2 cut(s)	664, 850
HincII	GTYRAC	1 cut(s)	1241
HindII	GTYRAC	1 cut(s)	1241
HindIII	AAGCTT	4 cut(s)	56, 379, 448, 2424
HinfI	GANTC	7 cut(s)	487, 643, 967, 1010, 1577, 2152, 2480
HpaII	CCGG	4 cut(s)	1391, 2178, 2435, 2581
HphI	GGTGA	4 cut(s)	893, 1238, 1958, 2000
Hpy166II	GTNNAC	5 cut(s)	671, 748, 1241, 1564, 1949
Hpy8I	GTNNAC	5 cut(s)	671, 748, 1241, 1564, 1949
HpyAV	CCTTC	9 cut(s)	358, 452, 731, 779, 967, 971, 1646, 1937, 2366
HpyCH4III	ACNGT	4 cut(s)	496, 1310, 2260, 2286
HpyCH4IV	ACGT	7 cut(s)	433, 779, 1318, 1439, 1741, 2131, 2557
HpySE526I	ACGT	7 cut(s)	433, 779, 1318, 1439, 1741, 2131, 2557
Hsp92I	GRCGYC	1 cut(s)	850
HspAI	GCGC	2 cut(s)	664, 850
KasI	GGCGCC	1 cut(s)	849
Kpn2I	TCCGGA	2 cut(s)	2177, 2434
KpnI	GGTACC	1 cut(s)	1433
Ksp22I	TGATCA	1 cut(s)	388
LmnI	GCTCC	7 cut(s)	79, 172, 574, 1135, 1397, 1661, 2583
MaeI	CTAG	6 cut(s)	567, 587, 992, 1707, 2309, 2520
MaeII	ACGT	7 cut(s)	433, 779, 1318, 1439, 1741, 2131, 2557
MaeIII	GTNAC	3 cut(s)	484, 1266, 2127
MbiI	CCGCTC	3 cut(s)	74, 83, 2006
MboII	GAAGA	9 cut(s)	89, 271, 317, 764, 769, 803, 1457, 1836, 1952
MfeI	CAATTG	1 cut(s)	1620
MflI	RGATCY	4 cut(s)	1050, 1727, 2187, 2365
MlsI	TGGCCA	2 cut(s)	1806, 2554
MluNI	TGGCCA	2 cut(s)	1806, 2554
Mly113I	GGCGCC	1 cut(s)	850
MlyI	GAGTC	2 cut(s)	481, 2161
MmeI	TCCRAC	4 cut(s)	99, 913, 1375, 1422
Mox20I	TGGCCA	2 cut(s)	1806, 2554
Mph1103I	ATGCAT	1 cut(s)	730
MroI	TCCGGA	2 cut(s)	2177, 2434
MroXI	GAANNNNTTC	2 cut(s)	814, 1212
MscI	TGGCCA	2 cut(s)	1806, 2554
MslI	CAYNNNNRTG	2 cut(s)	1412, 2393
Msp20I	TGGCCA	2 cut(s)	1806, 2554
MspA1I	CMGCKG	5 cut(s)	27, 347, 501, 1376, 1556
MspCI	CTTAAG	3 cut(s)	242, 451, 2407
MspI	CCGG	4 cut(s)	1391, 2178, 2435, 2581
MspR9I	CCNGG	5 cut(s)	475, 516, 857, 1391, 2581
MunI	CAATTG	1 cut(s)	1620
Mva1269I	GAATGC	1 cut(s)	2069
MvaI	CCWGG	3 cut(s)	475, 516, 857
MvnI	CGCG	2 cut(s)	666, 1015
NarI	GGCGCC	1 cut(s)	850
NciI	CCSGG	2 cut(s)	1391, 2581
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	2 cut(s)	393, 1249
NheI	GCTAGC	1 cut(s)	2519
NlaIV	GGNNCC	8 cut(s)	168, 239, 767, 851, 1170, 1431, 1506, 1643
NmeAIII	GCCGAG	1 cut(s)	1201
NmuCI	GTSAC	2 cut(s)	484, 2127
NsiI	ATGCAT	1 cut(s)	730
NspI	RCATGY	2 cut(s)	754, 1277
OliI	CACNNNNGTG	1 cut(s)	2393
PaeI	GCATGC	1 cut(s)	1277
PcsI	WCGNNNNNNNCGW	1 cut(s)	1237
PctI	GAATGC	1 cut(s)	2069
PdmI	GAANNNNTTC	2 cut(s)	814, 1212
PfeI	GAWTC	5 cut(s)	643, 967, 1010, 1577, 2480
PflFI	GACNNNGTC	1 cut(s)	2310
PflMI	CCANNNNNTGG	1 cut(s)	331
Ple19I	CGATCG	1 cut(s)	2141
PleI	GAGTC	2 cut(s)	481, 2160
PluTI	GGCGCC	1 cut(s)	853
PmaCI	CACGTG	2 cut(s)	2132, 2558
PmlI	CACGTG	2 cut(s)	2132, 2558
PpsI	GAGTC	2 cut(s)	481, 2160
Ppu21I	YACGTR	4 cut(s)	1319, 1742, 2132, 2558
PpuMI	RGGWCCY	3 cut(s)	238, 1504, 1642
PshBI	ATTAAT	1 cut(s)	1152
Psp5II	RGGWCCY	3 cut(s)	238, 1504, 1642
Psp6I	CCWGG	3 cut(s)	473, 514, 855
PspCI	CACGTG	2 cut(s)	2132, 2558
PspGI	CCWGG	3 cut(s)	473, 514, 855
PspN4I	GGNNCC	8 cut(s)	168, 239, 767, 851, 1170, 1431, 1506, 1643
PspPPI	RGGWCCY	3 cut(s)	238, 1504, 1642
PsrI	GAACNNNNNNTAC	2 cut(s)	2365, 2397
PstI	CTGCAG	2 cut(s)	318, 1795
PsuI	RGATCY	4 cut(s)	1050, 1727, 2187, 2365
PsyI	GACNNNGTC	1 cut(s)	2310
PvuI	CGATCG	1 cut(s)	2141
PvuII	CAGCTG	4 cut(s)	27, 347, 1376, 1556
RsaI	GTAC	3 cut(s)	582, 1431, 2567
RsaNI	GTAC	3 cut(s)	581, 1430, 2566
RseI	CAYNNNNRTG	2 cut(s)	1412, 2393
SalI	GTCGAC	1 cut(s)	1239
SchI	GAGTC	2 cut(s)	481, 2161
ScrFI	CCNGG	5 cut(s)	475, 516, 857, 1391, 2581
SfcI	CTRYAG	4 cut(s)	314, 687, 1344, 1791
SfoI	GGCGCC	1 cut(s)	851
SinI	GGWCC	9 cut(s)	30, 112, 238, 322, 560, 1092, 1504, 1642, 2437
SmiMI	CAYNNNNRTG	2 cut(s)	1412, 2393
SmlI	CTYRAG	9 cut(s)	140, 242, 451, 605, 1572, 2226, 2273, 2407, 2503
SmoI	CTYRAG	9 cut(s)	140, 242, 451, 605, 1572, 2226, 2273, 2407, 2503
SphI	GCATGC	1 cut(s)	1277
SspDI	GGCGCC	1 cut(s)	849
SspI	AATATT	2 cut(s)	1772, 2207
SspMI	CTAG	6 cut(s)	567, 587, 992, 1707, 2309, 2520
StyD4I	CCNGG	5 cut(s)	473, 514, 855, 1389, 2579
StyI	CCWWGG	1 cut(s)	1636
TaaI	ACNGT	4 cut(s)	496, 1310, 2260, 2286
TaiI	ACGT	7 cut(s)	436, 782, 1321, 1442, 1744, 2134, 2560
TaqI	TCGA	7 cut(s)	63, 952, 1008, 1240, 1305, 2155, 2371
TaqII	GACCGA	2 cut(s)	23, 47
TauI	GCSGC	3 cut(s)	74, 1193, 2006
TfiI	GAWTC	5 cut(s)	643, 967, 1010, 1577, 2480
TscAI	CASTG	4 cut(s)	750, 1147, 2395, 2460
TseFI	GTSAC	2 cut(s)	484, 2127
Tsp45I	GTSAC	2 cut(s)	484, 2127
TspGWI	ACGGA	2 cut(s)	1084, 1512
TspRI	CASTG	4 cut(s)	750, 1147, 2395, 2460
Tth111I	GACNNNGTC	1 cut(s)	2310
Van91I	CCANNNNNTGG	1 cut(s)	331
Vha464I	CTTAAG	3 cut(s)	242, 451, 2407
VpaK11BI	GGWCC	9 cut(s)	30, 112, 238, 322, 560, 1092, 1504, 1642, 2437
VspI	ATTAAT	1 cut(s)	1152
XapI	RAATTY	7 cut(s)	106, 937, 948, 1212, 1469, 1855, 2049
XbaI	TCTAGA	1 cut(s)	991
XceI	RCATGY	2 cut(s)	754, 1277
XmiI	GTMKAC	2 cut(s)	1240, 1563
XmnI	GAANNNNTTC	2 cut(s)	814, 1212
XspI	CTAG	6 cut(s)	567, 587, 992, 1707, 2309, 2520
Zsp2I	ATGCAT	1 cut(s)	730

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.