Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005404

Physical Location & Seq

Genomic Coordinates Reverse (-)

8681861 .. 8695666

Gene Structure 13806 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1076700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGGCGACGGAGCAAAACATGAGTAGCTGGACCCATCTGCTCCACTCCTCCACCAAGCTTCTCGAACAAGCGGCGCCTTCCGCTCAGTTCCCTCCGCTACAGAGGAATTTAGATCAGTTAGAAACATTATCAAAGAAACTCAAGGCGAAAACTCTACGAAATGAGGCTCCTCAACAATCCGTTGCTGCCACAAGGCTTCTTGCACGTGAGGGACTAAATGCAGAGCAGCTTGCACGTGACCTTAAGTCCTTTGAATTGAAGACAACATTTGAAGATGTTTTTCCGTCTGAAGCAACAAATGTTGAAGAGTATCTGCAGCAGGTCCATCAAATGGCAATGGTCTCAGCTCTTCAAGAAGCTCAGAAGGATAACCTTAGAAGTTTTAATGATTACATGTTAAAAGTTTTGGAGGAGGATTGGCAAAAGGAAAAACGTGATTTCCTTCAAAGCTTAAGCCAGATTTCGACGTTACCCAGGACCAACATGACTTATACTAGCAGCGGTGGTAGCCATTCTGGTCAAATAGCATCCATAACTTCTAGCCCTCAAGTTTCATCTTATCCATCTAGCATGGAGCTTGTACATTTAGAAAGCAAGCCCATCCGTGACAAGAAAGCCTCTGTCTATGCTGAAGTTGTAAAAAGTCTGAATAATGCAAGGCAGCGCGGATTACCGTATAAGCCCGCCACAGCTTTCAGGGGTGCTTATGAGAGTTTAGGCCTTGATGGATCTGGTGGAAAATCAGTTAACATGCAGAAGATATGGCACCTTCTTCAGACGTTGATGGGTGAAGATATAACCCTACCGCGTAGCGTTTCAAAAAGGATGTCATTAGTTATTGGGGCAAGGCGCCACCTAGAATGGGGGCATGAAAAATACATCATGGATACAATTCAAAGTCATCCGGTGCAGGCTGCCCTGGGAGGAGTTGTTGGAAATTTACAAAGAATCCGTGCCTTTCTTCGGATTCGTTTAAGAGATTATGGAGTTCTAGATTTTGATGCGGGAGATGCTCGTAGGCAGCCTCCTGTTGATACCACTTGGCAGCAGCTCTACTTTTGCTTGAGAACTGGGTATTACGATGAAGCAAGATCTGTTGCTCTGTCATCTCGTGCTTCACACCAGTTTGCTCCTCTGCTTACGGAGTGGATTAATACTGGGGGTATGGTGCCTGCGGCCATTGCAGCTTCTGCGTCAGAAGAATGTGAAAAAATGTTAAGAATGGTTGATCGTGTGGGCCGAGCTGCATATGACAAGAAAAAGTTGTTATTATATGCTCTAGTATCTGGTTCTCGTAAGCAAATCGACCGTCTACTAAGAGATCTACCAACGCTTTTCAACACTATAGAGGATTTCTTGTGGTTCAAATTGTCAGCTGTAAGAGATTGCCCTGGTGGAGGTGCCCCCATTGTTATGAATGAGAGCTTGGTACCATACACATTGGATGATTTGCAGATTTACTTAAATAAATTTGACCCGTCATATTATACAAAAAATGGAAAGGACCCCCTTGTGTACCCATACGTTTTGCTTTTAAGCATCCAATTGATACCAGGTGTGCTATACTTGTTTAAAGAAACTGGAGATGAGGGATATAACATTGATACTGCCCACATATCAATTGTGCTAGCAGATCATGGGGTCCTTTCTGAAGGTGCTGGTGCTGGACAGAAAATGGGCGTGATGGATGCTTACGCAGAGGCATCAAACATAATCAGGCAGTATGGATCTGTGTATTTACGTCTTGGTAATCTATCAATGGCATTAGAATATTATGCACAAGCTGCTGCTGCGGTTGGTGGTGGGGAATTGTCATGGTCTGGAAGAGGTAACGTTGATCAGCAAAGGCAGAGAAATTTGATGCTGAAGCAGCTCCTTACAGAGCTGTTGTTGCGGGATGGTGGGATCTATTTATTACTTGGTTCAAGAGGTGCCGGAGAAGGTGATTTGGGTCGATTTTTGACTGATGCAAAAGCAAGGCAACAATTTCTTCTTGAAGCTGCACACCAATGTCAAGAAGCTGGGTTGTATGAAAAGTCTATAGAAATTCAGAAACGAATAGGAGCATTCTCGATGGCATTGGATACTATTAACAAGTGCCTCTCTGAAGCAATTTGTGCCCTTTCACGTGGTAGATTGGATGGTGATAGTCGAACTGCTGGCCTCATTCACTCTGGAAATGAGATCTTGGAAATGCACAAATATTTTCCTGATATCAGTCCTCAAGAGAGGGAAATTGTTTCTGAGCAGTACATTGTTTTAAGACAACTTGAGGCCGTACTGTCAATCCATAAGTTGGCAAGAGGAGGCTGTTATGCTGATGCTTTAAGGGAGGTTGCCAGGCTTCAATTTCTTCCATTAGTTCCCGGGACACCAGATGCTACCACCGATGTCTTCCAGAATTTATCTCCGCATGTCCAAGCCTGTGTTCCAGACCTCTTGAAGGTTGCTCTCACTTGTCTGGACAACATGGTGGATTCTGATGGATCGCTTCGTGCCTTGAGGGCTAAGATTGCTAGCTTCATTGCAAATAATTCCAATAGGAACTGGCCTCGTGATTTGTATGAGAGAGTTGCCCGGAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016973 GO:0017038 GO:0017056 GO:0022607 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0046931 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051292 GO:0051641 GO:0051649 GO:0065003 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nic96

Protein Analysis

863

Amino Acids

95.97

Weight (kDa)

7.26

Isoelectric Point (pI)

46.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nic96	PF04097	250 - 856	9.4e-96	Nup93/Nic96

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005404)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41620 AT3G57350
fragaria_vesca	FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390
malus_domestica	MD00G1107400.v1.1 MD12G1080600.v1.1 MD14G1076700.v1.1
prunus_persica	Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1
pyrus_communis	pycom12g06710 pycom14g06500
rosa_chinensis	RchiOBHm_Chr3g0483381
rosa_laevigata	RLG00000023288
rosa_multiflora	Rmu_sc0006349.1_g000012
rosa_roxburghii	Rroxscaffold_6G00398400
rosa_rugosa	Rorug03G0205600
rosa_samantha	Rh3AG254000 Rh3CG288100 Rh3DG283900
rosa_wichuraiana	Rw0G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	310
Acc65I	GGTACC	1 cut(s)	1429
AccB1I	GGYRCC	7 cut(s)	73, 765, 849, 1168, 1400, 1429, 1931
AccB7I	CCANNNNNTGG	2 cut(s)	331, 2296
AccBSI	CCGCTC	1 cut(s)	83
AccI	GTMKAC	1 cut(s)	1312
AccII	CGCG	2 cut(s)	666, 808
AclI	AACGTT	1 cut(s)	1833
AclWI	GGATC	4 cut(s)	736, 1735, 1913, 2494
AcoI	YGGCCR	1 cut(s)	1176
AcsI	RAATTY	6 cut(s)	106, 937, 1469, 1855, 2046, 2401
AcuI	CTGAAG	6 cut(s)	309, 651, 758, 1671, 1886, 2127
AcvI	CACGTG	3 cut(s)	206, 236, 2129
AcyI	GRCGYC	2 cut(s)	74, 850
AfaI	GTAC	5 cut(s)	582, 1431, 1517, 2252, 2280
AfiI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 1397, 2296, 2443
AflII	CTTAAG	2 cut(s)	242, 451
AflIII	ACRYGT	1 cut(s)	393
AhdI	GACNNNNNGTC	1 cut(s)	244
AjnI	CCWGG	5 cut(s)	473, 918, 1390, 1552, 2339
AjuI	GAANNNNNNNTTGG	4 cut(s)	1409, 1441, 2412, 2444
Alw26I	GTCTC	1 cut(s)	346
AlwI	GGATC	4 cut(s)	736, 1735, 1913, 2494
AlwNI	CAGNNNCTG	1 cut(s)	1190
Ama87I	CYCGRG	1 cut(s)	2366
AoxI	GGCC	6 cut(s)	718, 1176, 1237, 2161, 2274, 2549
ApoI	RAATTY	6 cut(s)	106, 937, 1469, 1855, 2046, 2401
ArsI	GACNNNNNNTTYG	4 cut(s)	812, 844, 1294, 1326
AseI	ATTAAT	1 cut(s)	1152
Asp718I	GGTACC	1 cut(s)	1429
AspLEI	GCGC	3 cut(s)	76, 666, 852
AspS9I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
AsuC2I	CCSGG	3 cut(s)	2367, 2368, 2578
AsuHPI	GGTGA	3 cut(s)	800, 1955, 2156
AsuNHI	GCTAGC	2 cut(s)	1627, 2516
AvaI	CYCGRG	1 cut(s)	2366
AvaII	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
BaeGI	GKGCMC	2 cut(s)	1405, 2122
BanI	GGYRCC	7 cut(s)	73, 765, 849, 1168, 1400, 1429, 1931
BauI	CACGAG	2 cut(s)	1110, 2553
BbrPI	CACGTG	3 cut(s)	206, 236, 2129
BbsI	GAAGAC	2 cut(s)	266, 2386
BceAI	ACGGC	1 cut(s)	2261
BciT130I	CCWGG	5 cut(s)	475, 920, 1392, 1554, 2341
BciVI	GTATCC	2 cut(s)	880, 2077
BclI	TGATCA	1 cut(s)	1837
BcnI	CCSGG	3 cut(s)	2367, 2368, 2578
BcoDI	GTCTC	1 cut(s)	346
BfaI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2517
BfmI	CTRYAG	4 cut(s)	98, 314, 1344, 2040
BfoI	RGCGCY	2 cut(s)	77, 853
BfrI	CTTAAG	2 cut(s)	242, 451
BfuAI	ACCTGC	1 cut(s)	310
BfuI	GTATCC	2 cut(s)	880, 2077
BglI	GCCNNNNNGGC	1 cut(s)	2504
BglII	AGATCT	3 cut(s)	1091, 1321, 2184
Bme1390I	CCNGG	8 cut(s)	475, 920, 1392, 1554, 2341, 2367, 2368, 2578
Bme18I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
BmeRI	GACNNNNNGTC	1 cut(s)	244
BmeT110I	CYCGRG	1 cut(s)	2366
BmgT120I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
BmrFI	CCNGG	8 cut(s)	475, 920, 1392, 1554, 2341, 2367, 2368, 2578
BmrI	ACTGGG	2 cut(s)	1080, 1167
BmtI	GCTAGC	2 cut(s)	1631, 2520
BmuI	ACTGGG	2 cut(s)	1080, 1167
BpiI	GAAGAC	2 cut(s)	266, 2386
BpmI	CTGGAG	1 cut(s)	1602
BpuEI	CTTGAG	6 cut(s)	125, 531, 1084, 2208, 2291, 2521
BpuMI	CCSGG	3 cut(s)	2367, 2368, 2578
BsaAI	YACGTR	3 cut(s)	206, 236, 2129
BsaHI	GRCGYC	2 cut(s)	74, 850
BsaI	GGTCTC	1 cut(s)	346
BsaJI	CCNNGG	5 cut(s)	473, 918, 919, 1390, 2366
BsaWI	WCCGGW	1 cut(s)	904
BsaXI	ACNNNNNCTCC	2 cut(s)	915, 945
Bsc4I	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 1397, 2296, 2443
Bse1I	ACTGG	5 cut(s)	1075, 1123, 1162, 1585, 2552
Bse3DI	GCAATG	3 cut(s)	342, 1179, 2523
BseBI	CCWGG	5 cut(s)	475, 920, 1392, 1554, 2341
BseDI	CCNNGG	5 cut(s)	473, 918, 919, 1390, 2366
BseGI	GGATG	9 cut(s)	527, 600, 831, 901, 1450, 1539, 1693, 1903, 2146
BseLI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 1397, 2296, 2443
BseMI	GCAATG	3 cut(s)	342, 1179, 2523
BseMII	CTCAG	4 cut(s)	98, 357, 374, 2235
BseNI	ACTGG	5 cut(s)	1075, 1123, 1162, 1585, 2552
BseRI	GAGGAG	6 cut(s)	37, 159, 425, 939, 1122, 2319
BseSI	GKGCMC	2 cut(s)	1405, 2122
BseYI	CCCAGC	1 cut(s)	2021
BsgI	GTGCAG	2 cut(s)	929, 1986
Bsh1236I	CGCG	2 cut(s)	666, 808
Bsh1285I	CGRYCG	1 cut(s)	1309
BshFI	GGCC	6 cut(s)	720, 1178, 1239, 2163, 2276, 2551
BshNI	GGYRCC	7 cut(s)	73, 765, 849, 1168, 1400, 1429, 1931
BsiEI	CGRYCG	1 cut(s)	1309
BsiHKCI	CYCGRG	1 cut(s)	2366
BsiSI	CCGG	4 cut(s)	905, 1935, 2367, 2578
BslFI	GGGAC	2 cut(s)	225, 2383
BslI	CCNNNNNNNGG	6 cut(s)	331, 862, 963, 1397, 2296, 2443
BsmAI	GTCTC	1 cut(s)	346
BsmFI	GGGAC	2 cut(s)	225, 2383
BsmI	GAATGC	1 cut(s)	2066
BsnI	GGCC	6 cut(s)	720, 1178, 1239, 2163, 2276, 2551
Bso31I	GGTCTC	1 cut(s)	346
BsoBI	CYCGRG	1 cut(s)	2366
Bsp1286I	GDGCHC	2 cut(s)	1405, 2122
Bsp1407I	TGTACA	1 cut(s)	580
BspANI	GGCC	6 cut(s)	720, 1178, 1239, 2163, 2276, 2551
BspCNI	CTCAG	4 cut(s)	97, 356, 373, 2236
BspFNI	CGCG	2 cut(s)	666, 808
BspMAI	CTGCAG	1 cut(s)	318
BspMI	ACCTGC	1 cut(s)	310
BspOI	GCTAGC	2 cut(s)	1631, 2520
BspPI	GGATC	4 cut(s)	736, 1735, 1913, 2494
BspQI	GCTCTTC	1 cut(s)	354
BspT107I	GGYRCC	7 cut(s)	73, 765, 849, 1168, 1400, 1429, 1931
BspTI	CTTAAG	2 cut(s)	242, 451
BspTNI	GGTCTC	1 cut(s)	346
BsrBI	CCGCTC	1 cut(s)	83
BsrDI	GCAATG	3 cut(s)	342, 1179, 2523
BsrGI	TGTACA	1 cut(s)	580
BsrI	ACTGG	5 cut(s)	1075, 1123, 1162, 1585, 2552
BssECI	CCNNGG	5 cut(s)	473, 918, 919, 1390, 2366
BssNI	GRCGYC	2 cut(s)	74, 850
BssSI	CACGAG	2 cut(s)	1110, 2553
Bst2BI	CACGAG	2 cut(s)	1110, 2553
Bst2UI	CCWGG	5 cut(s)	475, 920, 1392, 1554, 2341
Bst4CI	ACNGT	3 cut(s)	675, 1310, 2283
Bst6I	CTCTTC	3 cut(s)	300, 354, 1819
BstACI	GRCGYC	2 cut(s)	74, 850
BstAFI	CTTAAG	2 cut(s)	242, 451
BstAPI	GCANNNNNTGC	3 cut(s)	1190, 1784, 2117
BstAUI	TGTACA	1 cut(s)	580
BstBAI	YACGTR	3 cut(s)	206, 236, 2129
BstC8I	GCNNGC	8 cut(s)	231, 596, 684, 912, 1173, 1629, 2161, 2518
BstDEI	CTNAG	7 cut(s)	84, 343, 360, 374, 1316, 2244, 2508
BstENI	CCTNNNNNAGG	2 cut(s)	1395, 2441
BstF5I	GGATG	9 cut(s)	527, 600, 831, 901, 1450, 1539, 1693, 1903, 2146
BstFNI	CGCG	2 cut(s)	666, 808
BstH2I	RGCGCY	2 cut(s)	77, 853
BstHHI	GCGC	3 cut(s)	76, 666, 852
BstMAI	GTCTC	1 cut(s)	346
BstMCI	CGRYCG	1 cut(s)	1309
BstNI	CCWGG	5 cut(s)	475, 920, 1392, 1554, 2341
BstNSI	RCATGY	3 cut(s)	397, 754, 2417
BstSCI	CCNGG	8 cut(s)	473, 918, 1390, 1552, 2339, 2365, 2366, 2576
BstSFI	CTRYAG	4 cut(s)	98, 314, 1344, 2040
BstSLI	GKGCMC	2 cut(s)	1405, 2122
BstUI	CGCG	2 cut(s)	666, 808
BstV2I	GAAGAC	2 cut(s)	266, 2386
BstX2I	RGATCY	6 cut(s)	728, 1091, 1321, 1727, 1905, 2184
BstYI	RGATCY	6 cut(s)	728, 1091, 1321, 1727, 1905, 2184
BsuI	GTATCC	2 cut(s)	880, 2077
BsuRI	GGCC	6 cut(s)	720, 1178, 1239, 2163, 2276, 2551
BtsCI	GGATG	9 cut(s)	527, 600, 831, 901, 1450, 1539, 1693, 1903, 2146
BveI	ACCTGC	1 cut(s)	310
Cac8I	GCNNGC	8 cut(s)	231, 596, 684, 912, 1173, 1629, 2161, 2518
CaiI	CAGNNNCTG	1 cut(s)	1190
CfoI	GCGC	3 cut(s)	76, 666, 852
Cfr13I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
Cfr9I	CCCGGG	1 cut(s)	2366
CseI	GACGC	1 cut(s)	1182
CsiI	ACCWGGT	1 cut(s)	1552
Csp6I	GTAC	5 cut(s)	581, 1430, 1516, 2251, 2279
CviQI	GTAC	5 cut(s)	581, 1430, 1516, 2251, 2279
DdeI	CTNAG	7 cut(s)	84, 343, 360, 374, 1316, 2244, 2508
DinI	GGCGCC	2 cut(s)	75, 851
DraI	TTTAAA	1 cut(s)	1573
DriI	GACNNNNNGTC	1 cut(s)	244
EaeI	YGGCCR	1 cut(s)	1176
Eam1104I	CTCTTC	3 cut(s)	300, 354, 1819
Eam1105I	GACNNNNNGTC	1 cut(s)	244
EarI	CTCTTC	3 cut(s)	300, 354, 1819
Eco147I	AGGCCT	1 cut(s)	720
Eco31I	GGTCTC	1 cut(s)	346
Eco32I	GATATC	1 cut(s)	2215
Eco47I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
Eco57I	CTGAAG	6 cut(s)	309, 651, 758, 1671, 1886, 2127
Eco72I	CACGTG	3 cut(s)	206, 236, 2129
Eco88I	CYCGRG	1 cut(s)	2366
EcoNI	CCTNNNNNAGG	2 cut(s)	1395, 2441
EcoO109I	RGGNCCY	2 cut(s)	1504, 1642
EcoRII	CCWGG	5 cut(s)	473, 918, 1390, 1552, 2339
EcoRV	GATATC	1 cut(s)	2215
EgeI	GGCGCC	2 cut(s)	75, 851
EheI	GGCGCC	2 cut(s)	75, 851
FaqI	GGGAC	2 cut(s)	225, 2383
FauI	CCCGC	3 cut(s)	691, 997, 1887
FauNDI	CATATG	1 cut(s)	1249
FbaI	TGATCA	1 cut(s)	1837
FblI	GTMKAC	1 cut(s)	1312
FokI	GGATG	9 cut(s)	514, 587, 838, 888, 1457, 1526, 1700, 1910, 2153
FspBI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2517
GlaI	GCGC	3 cut(s)	75, 665, 851
GsaI	CCCAGC	1 cut(s)	2025
GsuI	CTGGAG	1 cut(s)	1602
HaeII	RGCGCY	2 cut(s)	77, 853
HaeIII	GGCC	6 cut(s)	720, 1178, 1239, 2163, 2276, 2551
HapII	CCGG	4 cut(s)	905, 1935, 2367, 2578
HgaI	GACGC	1 cut(s)	1182
HhaI	GCGC	3 cut(s)	76, 666, 852
Hin1I	GRCGYC	2 cut(s)	74, 850
Hin6I	GCGC	3 cut(s)	74, 664, 850
HinP1I	GCGC	3 cut(s)	74, 664, 850
HincII	GTYRAC	1 cut(s)	748
HindII	GTYRAC	1 cut(s)	748
HindIII	AAGCTT	2 cut(s)	56, 448
HinfI	GANTC	3 cut(s)	948, 967, 2477
HpaI	GTTAAC	1 cut(s)	748
HpaII	CCGG	4 cut(s)	905, 1935, 2367, 2578
HphI	GGTGA	3 cut(s)	800, 1955, 2156
Hpy166II	GTNNAC	3 cut(s)	748, 1313, 1516
Hpy8I	GTNNAC	3 cut(s)	748, 1313, 1516
Hpy99I	CGWCG	2 cut(s)	10, 469
HpyAV	CCTTC	7 cut(s)	87, 358, 452, 779, 1646, 1934, 2437
HpyCH4III	ACNGT	3 cut(s)	675, 1310, 2283
HpyCH4IV	ACGT	9 cut(s)	205, 235, 433, 467, 779, 1524, 1741, 1833, 2128
HpyF3I	CTNAG	7 cut(s)	84, 343, 360, 374, 1316, 2244, 2508
HpySE526I	ACGT	9 cut(s)	205, 235, 433, 467, 779, 1524, 1741, 1833, 2128
Hsp92I	GRCGYC	2 cut(s)	74, 850
HspAI	GCGC	3 cut(s)	74, 664, 850
KasI	GGCGCC	2 cut(s)	73, 849
KpnI	GGTACC	1 cut(s)	1433
Ksp22I	TGATCA	1 cut(s)	1837
KspAI	GTTAAC	1 cut(s)	748
LguI	GCTCTTC	1 cut(s)	354
LmnI	GCTCC	8 cut(s)	10, 45, 172, 574, 1135, 1878, 2063, 2580
MabI	ACCWGGT	1 cut(s)	1552
MaeI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2517
MaeII	ACGT	9 cut(s)	205, 235, 433, 467, 779, 1524, 1741, 1833, 2128
MaeIII	GTNAC	4 cut(s)	236, 468, 605, 1829
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	2 cut(s)	1544, 1620
MflI	RGATCY	6 cut(s)	728, 1091, 1321, 1727, 1905, 2184
MhlI	GDGCHC	2 cut(s)	1405, 2122
Mly113I	GGCGCC	2 cut(s)	74, 850
MmeI	TCCRAC	1 cut(s)	913
MslI	CAYNNNNRTG	2 cut(s)	1412, 2008
MspA1I	CMGCKG	2 cut(s)	501, 1376
MspCI	CTTAAG	2 cut(s)	242, 451
MspI	CCGG	4 cut(s)	905, 1935, 2367, 2578
MspR9I	CCNGG	8 cut(s)	475, 920, 1392, 1554, 2341, 2367, 2368, 2578
MunI	CAATTG	2 cut(s)	1544, 1620
Mva1269I	GAATGC	1 cut(s)	2066
MvaI	CCWGG	5 cut(s)	475, 920, 1392, 1554, 2341
MvnI	CGCG	2 cut(s)	666, 808
NarI	GGCGCC	2 cut(s)	74, 850
NciI	CCSGG	3 cut(s)	2367, 2368, 2578
NdeI	CATATG	1 cut(s)	1249
NheI	GCTAGC	2 cut(s)	1627, 2516
NmeAIII	GCCGAG	1 cut(s)	1265
NmuCI	GTSAC	2 cut(s)	236, 605
NspI	RCATGY	3 cut(s)	397, 754, 2417
PasI	CCCWGGG	1 cut(s)	919
PceI	AGGCCT	1 cut(s)	720
PciI	ACATGT	1 cut(s)	393
PciSI	GCTCTTC	1 cut(s)	354
PcsI	WCGNNNNNNNCGW	1 cut(s)	1237
PctI	GAATGC	1 cut(s)	2066
PfeI	GAWTC	3 cut(s)	948, 967, 2477
PflMI	CCANNNNNTGG	2 cut(s)	331, 2296
PluTI	GGCGCC	2 cut(s)	77, 853
PmaCI	CACGTG	3 cut(s)	206, 236, 2129
PmlI	CACGTG	3 cut(s)	206, 236, 2129
Ppu21I	YACGTR	3 cut(s)	206, 236, 2129
PpuMI	RGGWCCY	2 cut(s)	1504, 1642
PscI	ACATGT	1 cut(s)	393
PshBI	ATTAAT	1 cut(s)	1152
Psp1406I	AACGTT	1 cut(s)	1833
Psp5II	RGGWCCY	2 cut(s)	1504, 1642
Psp6I	CCWGG	5 cut(s)	473, 918, 1390, 1552, 2339
PspCI	CACGTG	3 cut(s)	206, 236, 2129
PspFI	CCCAGC	1 cut(s)	2021
PspGI	CCWGG	5 cut(s)	473, 918, 1390, 1552, 2339
PspPI	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
PspPPI	RGGWCCY	2 cut(s)	1504, 1642
PstI	CTGCAG	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	1190
PsuI	RGATCY	6 cut(s)	728, 1091, 1321, 1727, 1905, 2184
PvuII	CAGCTG	1 cut(s)	1376
RsaI	GTAC	5 cut(s)	582, 1431, 1517, 2252, 2280
RsaNI	GTAC	5 cut(s)	581, 1430, 1516, 2251, 2279
RseI	CAYNNNNRTG	2 cut(s)	1412, 2008
SapI	GCTCTTC	1 cut(s)	354
Sau96I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
ScrFI	CCNGG	8 cut(s)	475, 920, 1392, 1554, 2341, 2367, 2368, 2578
SduI	GDGCHC	2 cut(s)	1405, 2122
SexAI	ACCWGGT	1 cut(s)	1552
SfcI	CTRYAG	4 cut(s)	98, 314, 1344, 2040
SfoI	GGCGCC	2 cut(s)	75, 851
SinI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
SmaI	CCCGGG	1 cut(s)	2368
SmiMI	CAYNNNNRTG	2 cut(s)	1412, 2008
SmlI	CTYRAG	8 cut(s)	140, 242, 451, 546, 1063, 2223, 2270, 2500
SmoI	CTYRAG	8 cut(s)	140, 242, 451, 546, 1063, 2223, 2270, 2500
SseBI	AGGCCT	1 cut(s)	720
SspDI	GGCGCC	2 cut(s)	73, 849
SspI	AATATT	2 cut(s)	1772, 2204
SspMI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2517
StuI	AGGCCT	1 cut(s)	720
StyD4I	CCNGG	8 cut(s)	473, 918, 1390, 1552, 2339, 2365, 2366, 2576
TaaI	ACNGT	3 cut(s)	675, 1310, 2283
TaiI	ACGT	9 cut(s)	208, 238, 436, 470, 782, 1527, 1744, 1836, 2131
TaqI	TCGA	6 cut(s)	63, 464, 1305, 1954, 2072, 2152
TatI	WGTACW	2 cut(s)	580, 2250
TauI	GCSGC	2 cut(s)	74, 1178
TfiI	GAWTC	3 cut(s)	948, 967, 2477
TseFI	GTSAC	2 cut(s)	236, 605
Tsp45I	GTSAC	2 cut(s)	236, 605
TspDTI	ATGAA	6 cut(s)	543, 885, 1098, 1430, 2046, 2512
TspGWI	ACGGA	6 cut(s)	23, 169, 273, 593, 941, 1157
TspMI	CCCGGG	1 cut(s)	2366
Van91I	CCANNNNNTGG	2 cut(s)	331, 2296
Vha464I	CTTAAG	2 cut(s)	242, 451
VpaK11BI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
VspI	ATTAAT	1 cut(s)	1152
XagI	CCTNNNNNAGG	2 cut(s)	1395, 2441
XapI	RAATTY	6 cut(s)	106, 937, 1469, 1855, 2046, 2401
XbaI	TCTAGA	1 cut(s)	991
XceI	RCATGY	3 cut(s)	397, 754, 2417
XmaI	CCCGGG	1 cut(s)	2366
XmiI	GTMKAC	1 cut(s)	1312
XspI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.