Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008265

Physical Location & Seq

Genomic Coordinates Reverse (-)

10015353 .. 10019456

Gene Structure 4104 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1082400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1428 bp

ATGGTGGGGAGCATTGAGGCTGTGAATCGTAATTTATACTCGAACGGATCTGTTCAGAACTTTAATGCATTGGAGGAGAAGCTTGACGAGCTCCGAATCCGCCTTGGAAAGGCTAATGGTGATCCTTTGAGAATTGTTGGTGTCGGTGCGGGTGCATGGGGCAGTGTTTTTGCTGCTGTGTTGCAGGATAGCTATGGTCAATTTCGAGACAAGGTTCAGATAAGGATATGGAGAAGACCGGGAAGAGCTGTTGATAGAGCCACAGCTGAACATCTTTTTGAAGTGATCAACTCGAGGGAGGATGTGTTGAGGAGGTTGATCCGGCGTTGTGCGTATTTGAAATATGTGGAGGCAAGGCTGGGGGATCGAACTCTTTATGCCGATGAGATTTTAAAAGATGGATTTTGTTTGAACATGATCGATACCCCTCTGTGTCCTTTGAAGGTTGTAACCAGTTTACAGGAGGCTGTTTGGGATGCTGATATTGTGGTGAATGGCTTGCCATCAACGGAAACAAAAGAGGTGTTTGAAGAGATAAGTAATTATTGGAAGGAAAGAATCACAGATCCTATTATCATCTCTTTGGCGAAAGGTATTGAGGCTGCACTGGAACCTCTCCCGCATATCATAACTCCCACGCGAATTATTCATCGGGCGACTGGTGTACCTATAGAGAACATACTTTATCTGGGTGGCCCAAACATTGCCTCAGAAATCTACAACAAGGAATATGCTAATGCTCGAATATGCGGAGCTGAGAAGTGGAGAAAACCTCTTGCAAAATTCTTAAGGCAACCCCATTTTATTGTCTGGGACAATAGTGATCTTGTTACACATGAAGTCATGGGTGGCTTAAAGAATGTCTATGCCATCGGAGCAGGGATGGTAGCAGCTCTCACAAAGGAAAGTGCTACCAGCAAATCTGTATACTTTGCACATTGTACCTCGGAGATGATATTTATCACTCATTTGCTGGCGGAAGAGCCCGAGAAACTTGCAGGGCCTTTGCTGGCTGACACATATGTAACCTTGTTGAAAGGTCGCAATGCGTGGTATGGGCAAATGTTAGCCAAAGGAGAACTAAACCGGGATATGGGTGAAAACATCAGTGGCAAGGGAATGATTCAGGGTGTCTCTGCAGTCAAAGCATTTTATGAACTTCTCAGTCAGTCGAGCCTAAATGTGTTGCATCCTGAGGAAAACAAGCCTGTTGCTCCCGTAGAGCTCTGCCCCATCTTGAAGACACTATATAAAATATTGATAACAAGGGAGCAATCATCACAAGCTATTCTTCAAGCGTTGAGGGATGAAAACTTGAACGATCCCAGGGAACGCATTGAGATTGCACAGAGCCATGTCTTCTATAGGCCTTCCCTTCTCGGACAGCCTTTGACTAGTTTCCGGTTGCAAGATAAAGTGGTAATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0036094 GO:0044424 GO:0044444 GO:0044464 GO:0048037 GO:0050662 GO:0051287 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00564 ko01110 ko04011 map00564 map01110 map04011

Pfam Domains

Protein Families

NAD_Gly3P_dh_C NAD_Gly3P_dh_N

Protein Analysis

476

Amino Acids

53.19

Weight (kDa)

6.78

Isoelectric Point (pI)

42.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_Gly3P_dh_N	PF01210	147 - 247	2.3e-10	NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus
NAD_Gly3P_dh_C	PF07479	275 - 429	9e-20	NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008265)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41540 AT2G41540 AT2G41540 AT2G41540
malus_domestica	MD12G1082400.v1.1 MD14G1077200.v1.1
prunus_persica	Prupe.7G053500_v2.0.a1 Prupe.7G053500_v2.0.a1 Prupe.7G053500_v2.0.a1 Prupe.7G053500_v2.0.a1
pyrus_communis	pycom12g06820 pycom14g06600
rosa_chinensis	RchiOBHm_Chr3g0483571
rosa_laevigata	RLG00000023266
rosa_multiflora	Rmu_sc0000555.1_g000014
rosa_roxburghii	Rroxscaffold_6G00398210
rosa_rugosa	Rorug03G0207100 Rorug03G0207200
rosa_samantha	Rh3AG255300 Rh3BG292500 Rh3CG290100 Rh3DG286000
rosa_wichuraiana	Rw3G023090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	927
AccII	CGCG	1 cut(s)	640
AciI	CCGC	5 cut(s)	100, 149, 620, 750, 977
AclWI	GGATC	6 cut(s)	55, 116, 313, 372, 560, 1316
AcsI	RAATTY	1 cut(s)	782
AfaI	GTAC	2 cut(s)	666, 943
AfiI	CCNNNNNNNGG	2 cut(s)	109, 1093
AflII	CTTAAG	1 cut(s)	787
AgsI	TTSAA	9 cut(s)	281, 340, 412, 442, 530, 1036, 1240, 1295, 1318
AhlI	ACTAGT	1 cut(s)	1394
AjnI	CCWGG	1 cut(s)	1325
AluBI	AGCT	9 cut(s)	82, 91, 192, 248, 266, 755, 893, 1225, 1286
AluI	AGCT	9 cut(s)	82, 91, 192, 248, 266, 755, 893, 1225, 1286
Alw21I	GWGCWC	2 cut(s)	93, 1227
Alw26I	GTCTC	2 cut(s)	201, 1138
AlwI	GGATC	6 cut(s)	55, 116, 313, 372, 560, 1316
Ama87I	CYCGRG	2 cut(s)	292, 986
AoxI	GGCC	3 cut(s)	694, 1001, 1367
ApeKI	GCWGC	3 cut(s)	173, 602, 890
ApoI	RAATTY	1 cut(s)	782
AspS9I	GGNCC	2 cut(s)	695, 1001
AsuC2I	CCSGG	2 cut(s)	240, 1088
AsuHPI	GGTGA	3 cut(s)	131, 502, 1109
AvaI	CYCGRG	2 cut(s)	292, 986
AxyI	CCTNAGG	1 cut(s)	1194
BaeI	ACNNNNGTAYC	2 cut(s)	648, 681
BanII	GRGCYC	3 cut(s)	93, 987, 1227
BbsI	GAAGAC	3 cut(s)	241, 1247, 1351
Bbv12I	GWGCWC	2 cut(s)	93, 1227
BbvI	GCAGC	3 cut(s)	160, 589, 902
BccI	CCATC	5 cut(s)	392, 511, 877, 878, 1241
BcgI	CGANNNNNNTGC	2 cut(s)	977, 1011
BciT130I	CCWGG	1 cut(s)	1327
BclI	TGATCA	1 cut(s)	285
BcnI	CCSGG	2 cut(s)	240, 1088
BcoDI	GTCTC	2 cut(s)	201, 1138
BcuI	ACTAGT	1 cut(s)	1394
BfaI	CTAG	1 cut(s)	1395
BfmI	CTRYAG	3 cut(s)	669, 1137, 1363
BfrI	CTTAAG	1 cut(s)	787
BisI	GCNGC	3 cut(s)	174, 603, 891
BlsI	GCNGC	3 cut(s)	175, 604, 892
Bme1390I	CCNGG	3 cut(s)	240, 1088, 1327
BmeT110I	CYCGRG	2 cut(s)	292, 986
BmgT120I	GGNCC	2 cut(s)	695, 1001
BmiI	GGNNCC	1 cut(s)	612
BmrFI	CCNGG	3 cut(s)	240, 1088, 1327
BmsI	GCATC	2 cut(s)	466, 1198
BpiI	GAAGAC	3 cut(s)	241, 1247, 1351
BplI	GAGNNNNNCTC	2 cut(s)	757, 789
BpuMI	CCSGG	2 cut(s)	240, 1088
Bsa29I	ATCGAT	1 cut(s)	420
BsaBI	GATNNNNATC	1 cut(s)	959
BsaJI	CCNNGG	4 cut(s)	103, 945, 1325, 1326
BsaWI	WCCGGW	1 cut(s)	1401
BsaXI	ACNNNNNCTCC	2 cut(s)	290, 320
Bsc4I	CCNNNNNNNGG	2 cut(s)	109, 1093
Bse1I	ACTGG	3 cut(s)	453, 612, 664
Bse21I	CCTNAGG	1 cut(s)	1194
Bse3DI	GCAATG	2 cut(s)	702, 1051
Bse8I	GATNNNNATC	1 cut(s)	959
BseBI	CCWGG	1 cut(s)	1327
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	4 cut(s)	103, 945, 1325, 1326
BseGI	GGATG	5 cut(s)	307, 481, 888, 1189, 1312
BseJI	GATNNNNATC	1 cut(s)	959
BseLI	CCNNNNNNNGG	2 cut(s)	109, 1093
BseMI	GCAATG	2 cut(s)	702, 1051
BseMII	CTCAG	4 cut(s)	723, 747, 1177, 1185
BseNI	ACTGG	3 cut(s)	453, 612, 664
BseRI	GAGGAG	2 cut(s)	89, 325
BseXI	GCAGC	3 cut(s)	160, 589, 902
BseYI	CCCAGC	1 cut(s)	358
BsgI	GTGCAG	1 cut(s)	588
Bsh1236I	CGCG	1 cut(s)	640
BshFI	GGCC	3 cut(s)	696, 1003, 1369
BshVI	ATCGAT	1 cut(s)	420
BsiHKAI	GWGCWC	2 cut(s)	93, 1227
BsiHKCI	CYCGRG	2 cut(s)	292, 986
BsiSI	CCGG	4 cut(s)	239, 322, 1087, 1402
BslFI	GGGAC	1 cut(s)	827
BslI	CCNNNNNNNGG	2 cut(s)	109, 1093
BsmAI	GTCTC	2 cut(s)	201, 1138
BsmFI	GGGAC	1 cut(s)	827
BsnI	GGCC	3 cut(s)	696, 1003, 1369
BsoBI	CYCGRG	2 cut(s)	292, 986
Bsp1286I	GDGCHC	3 cut(s)	93, 987, 1227
Bsp143I	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
BspACI	CCGC	5 cut(s)	100, 149, 620, 750, 977
BspANI	GGCC	3 cut(s)	696, 1003, 1369
BspCNI	CTCAG	4 cut(s)	722, 748, 1176, 1186
BspDI	ATCGAT	1 cut(s)	420
BspFNI	CGCG	1 cut(s)	640
BspLI	GGNNCC	1 cut(s)	612
BspMAI	CTGCAG	1 cut(s)	1141
BspPI	GGATC	6 cut(s)	55, 116, 313, 372, 560, 1316
BspQI	GCTCTTC	2 cut(s)	238, 975
BspTI	CTTAAG	1 cut(s)	787
BsrDI	GCAATG	2 cut(s)	702, 1051
BsrI	ACTGG	3 cut(s)	453, 612, 664
BssECI	CCNNGG	4 cut(s)	103, 945, 1325, 1326
BssMI	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
BssNAI	GTATAC	1 cut(s)	928
BssT1I	CCWWGG	1 cut(s)	103
Bst1107I	GTATAC	1 cut(s)	928
Bst2UI	CCWGG	1 cut(s)	1327
Bst6I	CTCTTC	3 cut(s)	238, 525, 975
BstAFI	CTTAAG	1 cut(s)	787
BstC8I	GCNNGC	3 cut(s)	500, 975, 1011
BstDEI	CTNAG	4 cut(s)	709, 756, 1163, 1194
BstENI	CCTNNNNNAGG	1 cut(s)	107
BstF5I	GGATG	5 cut(s)	307, 481, 888, 1189, 1312
BstFNI	CGCG	1 cut(s)	640
BstKTI	GATC	9 cut(s)	50, 124, 288, 321, 367, 420, 568, 826, 1324
BstMAI	GTCTC	2 cut(s)	201, 1138
BstMBI	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
BstMWI	GCNNNNNNNGC	3 cut(s)	88, 875, 1145
BstNI	CCWGG	1 cut(s)	1327
BstSCI	CCNGG	3 cut(s)	238, 1086, 1325
BstSFI	CTRYAG	3 cut(s)	669, 1137, 1363
BstUI	CGCG	1 cut(s)	640
BstV1I	GCAGC	3 cut(s)	160, 589, 902
BstV2I	GAAGAC	3 cut(s)	241, 1247, 1351
BstX2I	RGATCY	2 cut(s)	47, 565
BstYI	RGATCY	2 cut(s)	47, 565
BstZ17I	GTATAC	1 cut(s)	928
Bsu15I	ATCGAT	1 cut(s)	420
Bsu36I	CCTNAGG	1 cut(s)	1194
BsuRI	GGCC	3 cut(s)	696, 1003, 1369
BsuTUI	ATCGAT	1 cut(s)	420
BtsCI	GGATG	5 cut(s)	307, 481, 888, 1189, 1312
BtsI	GCAGTG	1 cut(s)	169
BtsIMutI	CAGTG	3 cut(s)	169, 605, 1114
Cac8I	GCNNGC	3 cut(s)	500, 975, 1011
Cfr13I	GGNCC	2 cut(s)	695, 1001
ClaI	ATCGAT	1 cut(s)	420
Csp6I	GTAC	2 cut(s)	665, 942
CviAII	CATG	5 cut(s)	156, 415, 836, 844, 1355
CviQI	GTAC	2 cut(s)	665, 942
DdeI	CTNAG	4 cut(s)	709, 756, 1163, 1194
DpnI	GATC	9 cut(s)	49, 123, 287, 320, 366, 419, 567, 825, 1323
DpnII	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
DraI	TTTAAA	1 cut(s)	393
Eam1104I	CTCTTC	3 cut(s)	238, 525, 975
EarI	CTCTTC	3 cut(s)	238, 525, 975
EciI	GGCGGA	2 cut(s)	89, 992
Ecl136II	GAGCTC	2 cut(s)	91, 1225
Eco130I	CCWWGG	1 cut(s)	103
Eco147I	AGGCCT	1 cut(s)	1369
Eco24I	GRGCYC	3 cut(s)	93, 987, 1227
Eco53kI	GAGCTC	2 cut(s)	91, 1225
Eco81I	CCTNAGG	1 cut(s)	1194
Eco88I	CYCGRG	2 cut(s)	292, 986
EcoICRI	GAGCTC	2 cut(s)	91, 1225
EcoNI	CCTNNNNNAGG	1 cut(s)	107
EcoO109I	RGGNCCY	1 cut(s)	1001
EcoRII	CCWGG	1 cut(s)	1325
EcoT14I	CCWWGG	1 cut(s)	103
EcoT22I	ATGCAT	1 cut(s)	70
EcoT38I	GRGCYC	3 cut(s)	93, 987, 1227
ErhI	CCWWGG	1 cut(s)	103
FaeI	CATG	5 cut(s)	159, 418, 839, 847, 1358
FalI	AAGNNNNNCTT	2 cut(s)	1275, 1307
FaqI	GGGAC	1 cut(s)	827
FatI	CATG	5 cut(s)	155, 414, 835, 843, 1354
FauI	CCCGC	2 cut(s)	142, 627
FauNDI	CATATG	1 cut(s)	1021
FbaI	TGATCA	1 cut(s)	285
FblI	GTMKAC	1 cut(s)	927
Fnu4HI	GCNGC	3 cut(s)	174, 603, 891
FokI	GGATG	5 cut(s)	314, 488, 895, 1176, 1319
FriOI	GRGCYC	3 cut(s)	93, 987, 1227
Fsp4HI	GCNGC	3 cut(s)	174, 603, 891
FspBI	CTAG	1 cut(s)	1395
GluI	GCNGC	3 cut(s)	174, 603, 891
GsaI	CCCAGC	1 cut(s)	362
HaeIII	GGCC	3 cut(s)	696, 1003, 1369
HapII	CCGG	4 cut(s)	239, 322, 1087, 1402
Hin1II	CATG	5 cut(s)	159, 418, 839, 847, 1358
HindIII	AAGCTT	1 cut(s)	80
HinfI	GANTC	4 cut(s)	25, 96, 558, 1123
HpaII	CCGG	4 cut(s)	239, 322, 1087, 1402
HphI	GGTGA	3 cut(s)	131, 502, 1109
Hpy166II	GTNNAC	3 cut(s)	458, 665, 928
Hpy188I	TCNGA	7 cut(s)	57, 95, 219, 712, 875, 949, 1382
Hpy188III	TCNNGA	3 cut(s)	206, 1193, 1237
Hpy8I	GTNNAC	3 cut(s)	458, 665, 928
HpyAV	CCTTC	4 cut(s)	436, 544, 1380, 1385
HpyF10VI	GCNNNNNNNGC	3 cut(s)	88, 875, 1145
HpyF3I	CTNAG	4 cut(s)	709, 756, 1163, 1194
Hsp92II	CATG	5 cut(s)	159, 418, 839, 847, 1358
Ksp22I	TGATCA	1 cut(s)	285
Kzo9I	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
LguI	GCTCTTC	2 cut(s)	238, 975
LmnI	GCTCC	6 cut(s)	9, 96, 752, 875, 1219, 1270
Lsp1109I	GCAGC	3 cut(s)	160, 589, 902
LweI	GCATC	2 cut(s)	466, 1198
MaeI	CTAG	1 cut(s)	1395
MaeIII	GTNAC	3 cut(s)	448, 829, 1024
MalI	GATC	9 cut(s)	49, 123, 287, 320, 366, 419, 567, 825, 1323
MboI	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
MboII	GAAGA	7 cut(s)	246, 255, 542, 992, 1252, 1283, 1351
MflI	RGATCY	2 cut(s)	47, 565
MhlI	GDGCHC	3 cut(s)	93, 987, 1227
MluCI	AATT	6 cut(s)	31, 132, 200, 541, 642, 782
Mph1103I	ATGCAT	1 cut(s)	70
MseI	TTAA	4 cut(s)	63, 392, 788, 854
MspA1I	CMGCKG	1 cut(s)	266
MspCI	CTTAAG	1 cut(s)	787
MspI	CCGG	4 cut(s)	239, 322, 1087, 1402
MspR9I	CCNGG	3 cut(s)	240, 1088, 1327
MvaI	CCWGG	1 cut(s)	1327
MvnI	CGCG	1 cut(s)	640
MwoI	GCNNNNNNNGC	3 cut(s)	88, 875, 1145
NciI	CCSGG	2 cut(s)	240, 1088
NdeI	CATATG	1 cut(s)	1021
NdeII	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
NlaIII	CATG	5 cut(s)	159, 418, 839, 847, 1358
NlaIV	GGNNCC	1 cut(s)	612
NsiI	ATGCAT	1 cut(s)	70
PaeR7I	CTCGAG	1 cut(s)	292
PasI	CCCWGGG	1 cut(s)	1326
PceI	AGGCCT	1 cut(s)	1369
PciSI	GCTCTTC	2 cut(s)	238, 975
PfeI	GAWTC	4 cut(s)	25, 96, 558, 1123
PkrI	GCNGC	3 cut(s)	175, 604, 892
Psp124BI	GAGCTC	2 cut(s)	93, 1227
Psp6I	CCWGG	1 cut(s)	1325
PspFI	CCCAGC	1 cut(s)	358
PspGI	CCWGG	1 cut(s)	1325
PspN4I	GGNNCC	1 cut(s)	612
PspPI	GGNCC	2 cut(s)	695, 1001
PspXI	VCTCGAGB	1 cut(s)	292
PstI	CTGCAG	1 cut(s)	1141
PsuI	RGATCY	2 cut(s)	47, 565
PvuII	CAGCTG	1 cut(s)	266
RsaI	GTAC	2 cut(s)	666, 943
RsaNI	GTAC	2 cut(s)	665, 942
SacI	GAGCTC	2 cut(s)	93, 1227
SapI	GCTCTTC	2 cut(s)	238, 975
SaqAI	TTAA	4 cut(s)	63, 392, 788, 854
SatI	GCNGC	3 cut(s)	174, 603, 891
Sau3AI	GATC	9 cut(s)	47, 121, 285, 318, 364, 417, 565, 823, 1321
Sau96I	GGNCC	2 cut(s)	695, 1001
ScrFI	CCNGG	3 cut(s)	240, 1088, 1327
SduI	GDGCHC	3 cut(s)	93, 987, 1227
SfaNI	GCATC	2 cut(s)	466, 1198
SfcI	CTRYAG	3 cut(s)	669, 1137, 1363
Sfr274I	CTCGAG	1 cut(s)	292
SlaI	CTCGAG	1 cut(s)	292
SmlI	CTYRAG	2 cut(s)	292, 787
SmoI	CTYRAG	2 cut(s)	292, 787
SpeI	ACTAGT	1 cut(s)	1394
Sse9I	AATT	6 cut(s)	31, 132, 200, 541, 642, 782
SseBI	AGGCCT	1 cut(s)	1369
SsiI	CCGC	5 cut(s)	100, 149, 620, 750, 977
SspI	AATATT	1 cut(s)	1257
SspMI	CTAG	1 cut(s)	1395
SstI	GAGCTC	2 cut(s)	93, 1227
StuI	AGGCCT	1 cut(s)	1369
StyD4I	CCNGG	3 cut(s)	238, 1086, 1325
StyI	CCWWGG	1 cut(s)	103
TaqI	TCGA	7 cut(s)	41, 205, 293, 367, 420, 742, 1172
TasI	AATT	6 cut(s)	31, 132, 200, 541, 642, 782
TfiI	GAWTC	4 cut(s)	25, 96, 558, 1123
Tru1I	TTAA	4 cut(s)	63, 392, 788, 854
Tru9I	TTAA	4 cut(s)	63, 392, 788, 854
TscAI	CASTG	3 cut(s)	169, 612, 1114
TseI	GCWGC	3 cut(s)	173, 602, 890
TspDTI	ATGAA	4 cut(s)	638, 852, 1170, 1323
TspGWI	ACGGA	2 cut(s)	60, 524
TspRI	CASTG	3 cut(s)	169, 612, 1114
Vha464I	CTTAAG	1 cut(s)	787
XagI	CCTNNNNNAGG	1 cut(s)	107
XapI	RAATTY	1 cut(s)	782
XhoI	CTCGAG	1 cut(s)	292
XmiI	GTMKAC	1 cut(s)	927
XspI	CTAG	1 cut(s)	1395
Zsp2I	ATGCAT	1 cut(s)	70

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.