Rorug03G0207100

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008265
Physical Location & Seq
Reverse (-)
18335901 .. 18336614
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0207100.1

Sequence Viewer

Length: 714 bp
ATGAGCGTGGACATTCTCAGTTGGAATTGCAGAGGGATCTGTAATCCAACCACAACTCGAGCCTTACGGGATCTGGTCAGCCAGAGTCGACCTCAGATCGTGTTTTTGTGCGAAACAAAAATAAGTCAATTGTCAGATTTCCAGGCCCTTCATAGGGATCTAGGGTTTGCTCATTTTAAAGAGGTGTTGAGCGAAGGCCAGGCCGGTGGCCTCGGAGTGTTCTGGACGGAAGATGTTGGTGTTCAGATACGCACGGTTTCGGCTCATCATATTGATATGGAGGTGTGTACAGGTCCAGGAGAACCACGCTGGCGTCTTACTGGTTTCTATGGCTTTGCGCGTACGTGTGAACGTGATCTTTCTTGGCAGCTGCTTAGGGAGTTATGTGACTTAGATTCCTTGCCTTGGCTGATTATTGGGGATTTTAACGAGATTCTGACTAATGGAGAAAAGTTGAGTGGTCCGATTTGTCCTGAGAGGCAAATGAGGGGCTTTAGAGAAGCTTTGGGGTATGCAGAACTTATTGATCTAGGGTTTCAAGGGTCCAAGATGACGTGGTGGAACTCTGAAACGCAGTTGCGTCTTGATAGAGCTGTTGCTACGAACTCATGGAGTGATATTTTTGGTTTCGCTAGGGTACGACATCTAGCACCGAGTGACTCGGACCATGTTCCCATCTTATTGCAAGCTAGTACTGCTCCTCTACCTTTGTGA

Protein Analysis

237

Amino Acids

26.69

Weight (kDa)

5.37

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 220 3e-17 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 88
AccII CGCG 1 cut(s) 340
AclWI GGATC 3 cut(s) 44, 78, 165
AcyI GRCGYC 1 cut(s) 313
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 4 cut(s) 289, 343, 639, 694
AfiI CCNNNNNNNGG 3 cut(s) 153, 154, 405
AflIII ACRYGT 1 cut(s) 344
AgsI TTSAA 1 cut(s) 539
AjiI CACGTC 1 cut(s) 555
AjnI CCWGG 3 cut(s) 141, 198, 295
AluBI AGCT 4 cut(s) 370, 503, 593, 689
AluI AGCT 4 cut(s) 370, 503, 593, 689
AlwI GGATC 3 cut(s) 44, 78, 165
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 4 cut(s) 144, 196, 201, 208
ApeKI GCWGC 2 cut(s) 367, 370
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 5 cut(s) 145, 293, 461, 543, 664
AvaI CYCGRG 1 cut(s) 57
AvaII GGWCC 4 cut(s) 293, 461, 543, 664
BbvI GCAGC 2 cut(s) 357, 379
BccI CCATC 1 cut(s) 683
BciT130I CCWGG 3 cut(s) 143, 200, 297
BfaI CTAG 5 cut(s) 161, 530, 633, 647, 690
BisI GCNGC 2 cut(s) 368, 371
BlsI GCNGC 2 cut(s) 369, 372
BmcAI AGTACT 1 cut(s) 694
Bme1390I CCNGG 3 cut(s) 143, 200, 297
Bme18I GGWCC 4 cut(s) 293, 461, 543, 664
BmeT110I CYCGRG 1 cut(s) 57
BmgBI CACGTC 1 cut(s) 555
BmgT120I GGNCC 5 cut(s) 145, 293, 461, 543, 664
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 3 cut(s) 143, 200, 297
BplI GAGNNNNNCTC 2 cut(s) 76, 108
Bpu10I CCTNAGC 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 313
BsaJI CCNNGG 2 cut(s) 211, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 153, 154, 405
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 325
BseBI CCWGG 3 cut(s) 143, 200, 297
BseDI CCNNGG 2 cut(s) 211, 404
BseLI CCNNNNNNNGG 3 cut(s) 153, 154, 405
BseMII CTCAG 3 cut(s) 31, 107, 465
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 1 cut(s) 690
BseXI GCAGC 2 cut(s) 357, 379
Bsh1236I CGCG 1 cut(s) 340
BshFI GGCC 4 cut(s) 146, 198, 203, 210
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 204
BsiWI CGTACG 1 cut(s) 341
BslI CCNNNNNNNGG 3 cut(s) 153, 154, 405
BsnI GGCC 4 cut(s) 146, 198, 203, 210
BsoBI CYCGRG 1 cut(s) 57
Bsp1407I TGTACA 1 cut(s) 287
Bsp143I GATC 6 cut(s) 36, 70, 96, 157, 355, 526
BspANI GGCC 4 cut(s) 146, 198, 203, 210
BspCNI CTCAG 3 cut(s) 30, 106, 466
BspFNI CGCG 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 544
BspPI GGATC 3 cut(s) 44, 78, 165
BsrFI RCCGGY 1 cut(s) 203
BsrGI TGTACA 1 cut(s) 287
BsrI ACTGG 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 2 cut(s) 211, 404
BssMI GATC 6 cut(s) 36, 70, 96, 157, 355, 526
BssNI GRCGYC 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 3 cut(s) 143, 200, 297
Bst4CI ACNGT 1 cut(s) 256
BstACI GRCGYC 1 cut(s) 313
BstAUI TGTACA 1 cut(s) 287
BstBAI YACGTR 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 311, 687
BstDEI CTNAG 5 cut(s) 17, 93, 374, 391, 474
BstFNI CGCG 1 cut(s) 340
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 6 cut(s) 39, 73, 99, 160, 358, 529
BstMBI GATC 6 cut(s) 36, 70, 96, 157, 355, 526
BstMWI GCNNNNNNNGC 1 cut(s) 695
BstNI CCWGG 3 cut(s) 143, 200, 297
BstSCI CCNGG 3 cut(s) 141, 198, 295
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 2 cut(s) 357, 379
BstX2I RGATCY 3 cut(s) 36, 70, 157
BstXI CCANNNNNNTGG 1 cut(s) 206
BstYI RGATCY 3 cut(s) 36, 70, 157
BsuRI GGCC 4 cut(s) 146, 198, 203, 210
BtrI CACGTC 1 cut(s) 555
Cac8I GCNNGC 2 cut(s) 311, 687
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 5 cut(s) 145, 293, 461, 543, 664
CseI GACGC 2 cut(s) 302, 569
Csp6I GTAC 4 cut(s) 288, 342, 638, 693
CviAII CATG 2 cut(s) 609, 668
CviQI GTAC 4 cut(s) 288, 342, 638, 693
DdeI CTNAG 5 cut(s) 17, 93, 374, 391, 474
DpnI GATC 6 cut(s) 38, 72, 98, 159, 357, 528
DpnII GATC 6 cut(s) 36, 70, 96, 157, 355, 526
DraI TTTAAA 1 cut(s) 178
DraIII CACNNNGTG 1 cut(s) 656
Eco130I CCWWGG 1 cut(s) 404
Eco47I GGWCC 4 cut(s) 293, 461, 543, 664
Eco88I CYCGRG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 145
EcoRII CCWGG 3 cut(s) 141, 198, 295
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 2 cut(s) 612, 671
FaiI YATR 8 cut(s) 153, 270, 278, 330, 385, 513, 610, 669
FatI CATG 2 cut(s) 608, 667
FblI GTMKAC 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 368, 371
Fsp4HI GCNGC 2 cut(s) 368, 371
FspBI CTAG 5 cut(s) 161, 530, 633, 647, 690
GlaI GCGC 1 cut(s) 339
GluI GCNGC 2 cut(s) 368, 371
HaeIII GGCC 4 cut(s) 146, 198, 203, 210
HapII CCGG 1 cut(s) 204
HgaI GACGC 2 cut(s) 302, 569
HhaI GCGC 1 cut(s) 340
Hin1I GRCGYC 1 cut(s) 313
Hin1II CATG 2 cut(s) 612, 671
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 89
HindII GTYRAC 1 cut(s) 89
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 4 cut(s) 85, 395, 433, 659
HpaII CCGG 1 cut(s) 204
Hpy166II GTNNAC 4 cut(s) 10, 89, 288, 350
Hpy188I TCNGA 8 cut(s) 96, 136, 215, 246, 438, 465, 568, 664
Hpy188III TCNNGA 3 cut(s) 223, 473, 584
Hpy8I GTNNAC 4 cut(s) 10, 89, 288, 350
HpyAV CCTTC 2 cut(s) 158, 188
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 3 cut(s) 344, 352, 554
HpyCH4V TGCA 3 cut(s) 30, 515, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 695
HpyF3I CTNAG 5 cut(s) 17, 93, 374, 391, 474
HpySE526I ACGT 3 cut(s) 344, 352, 554
Hsp92I GRCGYC 1 cut(s) 313
Hsp92II CATG 2 cut(s) 612, 671
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 6 cut(s) 36, 70, 96, 157, 355, 526
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 2 cut(s) 357, 379
MaeI CTAG 5 cut(s) 161, 530, 633, 647, 690
MaeII ACGT 3 cut(s) 344, 352, 554
MaeIII GTNAC 2 cut(s) 386, 656
MalI GATC 6 cut(s) 38, 72, 98, 159, 357, 528
MboI GATC 6 cut(s) 36, 70, 96, 157, 355, 526
MboII GAAGA 1 cut(s) 242
MfeI CAATTG 1 cut(s) 128
MflI RGATCY 3 cut(s) 36, 70, 157
MluCI AATT 2 cut(s) 25, 128
MlyI GAGTC 2 cut(s) 94, 653
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 8 cut(s) 26, 102, 175, 221, 274, 471, 480, 711
MseI TTAA 2 cut(s) 177, 426
MspA1I CMGCKG 1 cut(s) 370
MspI CCGG 1 cut(s) 204
MspR9I CCNGG 3 cut(s) 143, 200, 297
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 3 cut(s) 143, 200, 297
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 695
NdeII GATC 6 cut(s) 36, 70, 96, 157, 355, 526
NlaIII CATG 2 cut(s) 612, 671
NlaIV GGNNCC 1 cut(s) 544
NmuCI GTSAC 2 cut(s) 386, 656
PaeR7I CTCGAG 1 cut(s) 57
PfeI GAWTC 2 cut(s) 395, 433
Pfl23II CGTACG 1 cut(s) 341
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 2 cut(s) 369, 372
PleI GAGTC 2 cut(s) 93, 653
PpsI GAGTC 2 cut(s) 93, 653
Ppu21I YACGTR 1 cut(s) 345
Psp6I CCWGG 3 cut(s) 141, 198, 295
PspGI CCWGG 3 cut(s) 141, 198, 295
PspLI CGTACG 1 cut(s) 341
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 5 cut(s) 145, 293, 461, 543, 664
PspXI VCTCGAGB 1 cut(s) 57
PsuI RGATCY 3 cut(s) 36, 70, 157
PvuII CAGCTG 1 cut(s) 370
RsaI GTAC 4 cut(s) 289, 343, 639, 694
RsaNI GTAC 4 cut(s) 288, 342, 638, 693
SalI GTCGAC 1 cut(s) 87
SaqAI TTAA 2 cut(s) 177, 426
SatI GCNGC 2 cut(s) 368, 371
Sau3AI GATC 6 cut(s) 36, 70, 96, 157, 355, 526
Sau96I GGNCC 5 cut(s) 145, 293, 461, 543, 664
ScaI AGTACT 1 cut(s) 694
SchI GAGTC 2 cut(s) 94, 653
ScrFI CCNGG 3 cut(s) 143, 200, 297
Sfr274I CTCGAG 1 cut(s) 57
SinI GGWCC 4 cut(s) 293, 461, 543, 664
SlaI CTCGAG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 57
SmoI CTYRAG 1 cut(s) 57
Sse9I AATT 2 cut(s) 25, 128
SspMI CTAG 5 cut(s) 161, 530, 633, 647, 690
StyD4I CCNGG 3 cut(s) 141, 198, 295
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 3 cut(s) 347, 355, 557
TaqI TCGA 2 cut(s) 58, 88
TasI AATT 2 cut(s) 25, 128
TatI WGTACW 2 cut(s) 287, 692
TfiI GAWTC 2 cut(s) 395, 433
Tru1I TTAA 2 cut(s) 177, 426
Tru9I TTAA 2 cut(s) 177, 426
TseFI GTSAC 2 cut(s) 386, 656
TseI GCWGC 2 cut(s) 367, 370
Tsp45I GTSAC 2 cut(s) 386, 656
TspDTI ATGAA 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 242
VpaK11BI GGWCC 4 cut(s) 293, 461, 543, 664
XhoI CTCGAG 1 cut(s) 57
XmiI GTMKAC 1 cut(s) 88
XspI CTAG 5 cut(s) 161, 530, 633, 647, 690
ZrmI AGTACT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.