Rorug03G0207200

glycerol-3-phosphate dehydrogenase NAD(

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0008265
Physical Location & Seq
Reverse (-)
18337621 .. 18340050
2430 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0207200.1

Sequence Viewer

Length: 1818 bp
ATGGCTCGCTCCTCCCACCAACCTCCGTCAACCACCGTCGATGAAGATTCCTCCGCTTCGATCTCCGCTGTCCATCCCGACATAATTCAAACTCACATCCTCACCCGCCTCGACGGCCCCACTCTCGCCTCCGCCGCCTGCACCTCCTCCCAGCTCCACGCCCTCGCCTCCGACCACAGCATCTGGGCCAACATCTGCCACTCCACGTGGCCCTCCACCACAACGCCACGTCTCCGCCAAGTCATCTCCACCTTCCCCGACGGCGCCTTCTCTTTCTTCTCCGACTCCTTCCCGCTTCTCGCCAACTTAGACTCCGCCGCCGCCGCCGCAGATCATCACGACAGTCCGACTGAGCTAATCTCCGCCGTCGATATCTACCACGGTGAAAAGCTCATCTTCAGCAAGGTGGTGGAGACGGAGACTGTGAGCGGATGGTTTACGTGCTCGCCGTTCCGGATTGACCTGCTGGACCCCAAGGACGTGGTTCCGACGCCGGTGAAGTACCCGGTGGGGCAGGAGGCCGACACGTGTCGCGATCTGGGGGAGGAACTGAGGCTGAGCTGGATTCTGATCGACCTGGTGGGACGGCGCGCCATGAACCTCTCGAGCCACACGGCGGTTTCGGTGCAGCGGCACTGGCTGAGCGGTGAGGTGCACGCGCGGTTCGCGTCGATTCTTCCCGGCCAGAGAGGGACGGCGTCGGAGTTCGTGCAGTGCGGTATCGTGGTGACGTGCGGCGCTGGGTCGGAGGGAGGGGAGTTGCAGGTGAGGGAGGTGAGCCTGCAGGTGGAGGACATGGACGGAATGCATTTGAACGGGAAGGACGGTTTGGTAATTTTGCAGAGGGGGTTGGAGGGCAGAAAGGGGAGGAAGGGGAGGAGAGGGGAGGAGGGAAGGAGGAGATACGGAGACTACTTGGAGAGGAAGAGGGAAAGGAAGGAGAAGAAGATGAGGACAGAAGGGACATTAGACACTTTGTGTGTCACTTTTGGCGGTTTGTGGAACGGCGGTCCTGCTTGGTTGTCGCGACCTACACCTACTCCATTCATGGAGAAAATCTCAGGTTTCGGCCTCCTCACCTTCGTCTTCGTTGTCTTCGGAGGTAGTTCTTGTGAAAAGAGTTTGGGTGAGGCTGAGGCCGTGTTGGTGGGCCAACGAACTGCTGGTACTGCATCGGTGGTTGGCGGTGCTTACGGGGTGGAGTGGGATCCGGGTAGGAGCCATGGTGATCTCAGTGGGTCGCCTCTTTCCGGCGGTGCTGCTGAAGCGGTGGTGATTCGGTGGGTGCGAAAGGGAGCTTTCTTCTGGTTGGATGGCATGCCGTTGTCGGTGCCGGGTCCGACTAGTGGAGCTATTGGAGTTGACGACTGTTGGGATGGCGATCGGGTTTGCTTTCTGGCGTTTATCGCGGACGGTGGAGACTGGATTGGCTCCCAATCTGGGAGTCTGAGGACGTCCGGTGGTGTGGGGTGGCTGGGTTCGTTCAAGTCTAGTGTGATGGCAGCGGCCGTTACAAGATCGGCGCTCTCTGTGGGTTGGTGGTGGCTTCGAGTCCTAGATCTGGAGAGGACGACCGGCGCCGCTGCTGACGGAGATGGTGCACTCCGACGGTGGCGTAGCTCAGCTTGCCGGAGTCGAGCTGGTGGGTTGGGTGTCCTGAGCACTTGTTCAGGGGATTGGAATTGGGCCTATTGTCTGGTGTCATGGCCTGCATTAGGGCTTTTTGGCTCTAGGTCCTGGGCTTGGAGGGCCTGCTGGGCTTATTTAATTTTAAAAGGGATGGAAGGTGATTTATCATCTTCCATTCCTAGCTTTTAG

Protein Analysis

605

Amino Acids

65.08

Weight (kDa)

6.09

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 26 - 67 1e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 754, 775
AatII GACGTC 1 cut(s) 1457
Acc36I ACCTGC 3 cut(s) 471, 754, 775
AccB1I GGYRCC 3 cut(s) 263, 1330, 1577
AccBSI CCGCTC 2 cut(s) 429, 645
AccII CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
AccIII TCCGGA 1 cut(s) 453
AclWI GGATC 2 cut(s) 1202, 1215
AcoI YGGCCR 2 cut(s) 682, 1506
AcuI CTGAAG 2 cut(s) 382, 1284
AcvI CACGTG 2 cut(s) 207, 528
AcyI GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
AdeI CACNNNGTG 1 cut(s) 978
AfaI GTAC 2 cut(s) 503, 1168
AfiI CCNNNNNNNGG 8 cut(s) 616, 787, 1250, 1346, 1440, 1561, 1715, 1743
AflIII ACRYGT 2 cut(s) 525, 527
AgsI TTSAA 3 cut(s) 89, 814, 1486
AhlI ACTAGT 1 cut(s) 1343
AjiI CACGTC 3 cut(s) 230, 481, 732
AjnI CCWGG 2 cut(s) 576, 1736
AjuI GAANNNNNNNTTGG 2 cut(s) 812, 844
Alw21I GWGCWC 4 cut(s) 446, 657, 1603, 1664
Alw26I GTCTC 5 cut(s) 236, 407, 413, 903, 1413
Alw44I GTGCAC 2 cut(s) 653, 1599
AlwI GGATC 2 cut(s) 1202, 1215
AlwNI CAGNNNCTG 1 cut(s) 183
Ama87I CYCGRG 1 cut(s) 604
Aor13HI TCCGGA 1 cut(s) 453
ApaLI GTGCAC 2 cut(s) 653, 1599
ApeKI GCWGC 4 cut(s) 628, 1259, 1502, 1583
AscI GGCGCGCC 1 cut(s) 589
AspLEI GCGC 7 cut(s) 266, 591, 593, 661, 740, 1525, 1580
AsuC2I CCSGG 4 cut(s) 506, 681, 1212, 1335
AvaI CYCGRG 1 cut(s) 604
AvaII GGWCC 4 cut(s) 469, 1010, 1337, 1734
BaeGI GKGCMC 2 cut(s) 657, 1603
BamHI GGATCC 1 cut(s) 1207
BanI GGYRCC 3 cut(s) 263, 1330, 1577
BbrPI CACGTG 2 cut(s) 207, 528
BbsI GAAGAC 2 cut(s) 1078, 1087
Bbv12I GWGCWC 4 cut(s) 446, 657, 1603, 1664
BbvCI CCTCAGC 1 cut(s) 1134
BbvI GCAGC 4 cut(s) 640, 1246, 1514, 1570
BccI CCATC 7 cut(s) 81, 426, 1307, 1370, 1492, 1589, 1774
BciT130I CCWGG 2 cut(s) 578, 1738
BcnI CCSGG 4 cut(s) 506, 681, 1212, 1335
BcoDI GTCTC 5 cut(s) 236, 407, 413, 903, 1413
BcuI ACTAGT 1 cut(s) 1343
BfaI CTAG 5 cut(s) 1344, 1491, 1556, 1731, 1809
BfmI CTRYAG 1 cut(s) 782
BfoI RGCGCY 4 cut(s) 267, 741, 1526, 1581
BfuAI ACCTGC 3 cut(s) 471, 754, 775
BglI GCCNNNNNGGC 2 cut(s) 114, 1757
BglII AGATCT 1 cut(s) 1558
BlpI GCTNAGC 3 cut(s) 557, 641, 1621
Bme1390I CCNGG 6 cut(s) 506, 578, 681, 1212, 1335, 1738
Bme18I GGWCC 4 cut(s) 469, 1010, 1337, 1734
BmeT110I CYCGRG 1 cut(s) 604
BmgBI CACGTC 3 cut(s) 230, 481, 732
BmrFI CCNGG 6 cut(s) 506, 578, 681, 1212, 1335, 1738
BmsI GCATC 2 cut(s) 189, 1181
BoxI GACNNNNGTC 1 cut(s) 528
BpiI GAAGAC 2 cut(s) 1078, 1087
BplI GAGNNNNNCTC 4 cut(s) 344, 376, 1043, 1075
BpmI CTGGAG 1 cut(s) 1583
Bpu10I CCTNAGC 2 cut(s) 1134, 1658
Bpu1102I GCTNAGC 3 cut(s) 557, 641, 1621
BpuMI CCSGG 4 cut(s) 506, 681, 1212, 1335
BsaAI YACGTR 3 cut(s) 207, 441, 528
BsaBI GATNNNNATC 2 cut(s) 569, 1380
BsaHI GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
BsaJI CCNNGG 4 cut(s) 379, 474, 1222, 1737
BsaWI WCCGGW 2 cut(s) 453, 1457
BsaXI ACNNNNNCTCC 6 cut(s) 296, 326, 1024, 1054, 1210, 1240
Bsc4I CCNNNNNNNGG 8 cut(s) 616, 787, 1250, 1346, 1440, 1561, 1715, 1743
Bse118I RCCGGY 2 cut(s) 493, 1574
Bse1I ACTGG 2 cut(s) 641, 1427
Bse8I GATNNNNATC 2 cut(s) 569, 1380
BseAI TCCGGA 1 cut(s) 453
BseBI CCWGG 2 cut(s) 578, 1738
BseDI CCNNGG 4 cut(s) 379, 474, 1222, 1737
BseGI GGATG 6 cut(s) 73, 96, 437, 1318, 1381, 1785
BseJI GATNNNNATC 2 cut(s) 569, 1380
BseLI CCNNNNNNNGG 8 cut(s) 616, 787, 1250, 1346, 1440, 1561, 1715, 1743
BseNI ACTGG 2 cut(s) 641, 1427
BsePI GCGCGC 1 cut(s) 589
BseRI GAGGAG 5 cut(s) 136, 892, 902, 913, 1064
BseSI GKGCMC 2 cut(s) 657, 1603
BseX3I CGGCCG 1 cut(s) 1506
BseXI GCAGC 4 cut(s) 640, 1246, 1514, 1570
BseYI CCCAGC 4 cut(s) 150, 740, 1474, 1755
BsgI GTGCAG 3 cut(s) 124, 647, 731
Bsh1236I CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
Bsh1285I CGRYCG 3 cut(s) 1384, 1509, 1575
BshNI GGYRCC 3 cut(s) 263, 1330, 1577
BsiEI CGRYCG 3 cut(s) 1384, 1509, 1575
BsiHKAI GWGCWC 4 cut(s) 446, 657, 1603, 1664
BsiHKCI CYCGRG 1 cut(s) 604
BslFI GGGAC 3 cut(s) 597, 706, 976
BslI CCNNNNNNNGG 8 cut(s) 616, 787, 1250, 1346, 1440, 1561, 1715, 1743
BsmAI GTCTC 5 cut(s) 236, 407, 413, 903, 1413
BsmBI CGTCTC 2 cut(s) 236, 407
BsmFI GGGAC 3 cut(s) 597, 706, 976
BsmI GAATGC 1 cut(s) 810
BsoBI CYCGRG 1 cut(s) 604
Bsp1286I GDGCHC 4 cut(s) 446, 657, 1603, 1664
Bsp13I TCCGGA 1 cut(s) 453
Bsp143I GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
Bsp1720I GCTNAGC 3 cut(s) 557, 641, 1621
Bsp19I CCATGG 1 cut(s) 1222
Bsp68I TCGCGA 2 cut(s) 534, 1027
BspEI TCCGGA 1 cut(s) 453
BspFNI CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
BspMAI CTGCAG 1 cut(s) 786
BspMI ACCTGC 3 cut(s) 471, 754, 775
BspPI GGATC 2 cut(s) 1202, 1215
BspT107I GGYRCC 3 cut(s) 263, 1330, 1577
BsrBI CCGCTC 2 cut(s) 429, 645
BsrFI RCCGGY 2 cut(s) 493, 1574
BsrI ACTGG 2 cut(s) 641, 1427
BssAI RCCGGY 2 cut(s) 493, 1574
BssECI CCNNGG 4 cut(s) 379, 474, 1222, 1737
BssHII GCGCGC 1 cut(s) 589
BssMI GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
BssNI GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
BssT1I CCWWGG 2 cut(s) 474, 1222
Bst2UI CCWGG 2 cut(s) 578, 1738
Bst4CI ACNGT 8 cut(s) 37, 344, 383, 424, 827, 1370, 1415, 1611
Bst6I CTCTTC 1 cut(s) 920
BstACI GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
BstBAI YACGTR 3 cut(s) 207, 441, 528
BstDSI CCRYGG 2 cut(s) 379, 1222
BstENI CCTNNNNNAGG 1 cut(s) 1713
BstF5I GGATG 6 cut(s) 73, 96, 437, 1318, 1381, 1785
BstFNI CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
BstH2I RGCGCY 4 cut(s) 267, 741, 1526, 1581
BstHHI GCGC 7 cut(s) 266, 591, 593, 661, 740, 1525, 1580
BstKTI GATC 9 cut(s) 63, 334, 538, 573, 1210, 1231, 1384, 1520, 1561
BstMAI GTCTC 5 cut(s) 236, 407, 413, 903, 1413
BstMBI GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
BstMCI CGRYCG 3 cut(s) 1384, 1509, 1575
BstNI CCWGG 2 cut(s) 578, 1738
BstNSI RCATGY 1 cut(s) 1321
BstPAI GACNNNNGTC 1 cut(s) 528
BstSCI CCNGG 6 cut(s) 504, 576, 679, 1210, 1333, 1736
BstSFI CTRYAG 1 cut(s) 782
BstSLI GKGCMC 2 cut(s) 657, 1603
BstUI CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
BstV1I GCAGC 4 cut(s) 640, 1246, 1514, 1570
BstV2I GAAGAC 2 cut(s) 1078, 1087
BstX2I RGATCY 2 cut(s) 1207, 1558
BstXI CCANNNNNNTGG 1 cut(s) 481
BstYI RGATCY 2 cut(s) 1207, 1558
BstZI CGGCCG 1 cut(s) 1506
BtgI CCRYGG 2 cut(s) 379, 1222
BtrI CACGTC 3 cut(s) 230, 481, 732
BtsCI GGATG 6 cut(s) 73, 96, 437, 1318, 1381, 1785
BtsI GCAGTG 1 cut(s) 719
BtsIMutI CAGTG 3 cut(s) 634, 719, 1240
BtuMI TCGCGA 2 cut(s) 534, 1027
BveI ACCTGC 3 cut(s) 471, 754, 775
CaiI CAGNNNCTG 1 cut(s) 183
CfoI GCGC 7 cut(s) 266, 591, 593, 661, 740, 1525, 1580
Cfr10I RCCGGY 2 cut(s) 493, 1574
CseI GACGC 3 cut(s) 499, 657, 687
CsiI ACCWGGT 1 cut(s) 576
Csp6I GTAC 2 cut(s) 502, 1167
CviAII CATG 6 cut(s) 595, 796, 1048, 1223, 1318, 1704
CviQI GTAC 2 cut(s) 502, 1167
DinI GGCGCC 2 cut(s) 265, 1579
DpnI GATC 9 cut(s) 62, 333, 537, 572, 1209, 1230, 1383, 1519, 1560
DpnII GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
DraI TTTAAA 1 cut(s) 1773
DraIII CACNNNGTG 1 cut(s) 978
EaeI YGGCCR 2 cut(s) 682, 1506
EagI CGGCCG 1 cut(s) 1506
Eam1104I CTCTTC 1 cut(s) 920
EarI CTCTTC 1 cut(s) 920
EciI GGCGGA 4 cut(s) 121, 224, 304, 352
EclXI CGGCCG 1 cut(s) 1506
Eco130I CCWWGG 2 cut(s) 474, 1222
Eco32I GATATC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 469, 1010, 1337, 1734
Eco52I CGGCCG 1 cut(s) 1506
Eco57I CTGAAG 2 cut(s) 382, 1284
Eco72I CACGTG 2 cut(s) 207, 528
Eco88I CYCGRG 1 cut(s) 604
EcoNI CCTNNNNNAGG 1 cut(s) 1713
EcoO109I RGGNCCY 2 cut(s) 1734, 1749
EcoRII CCWGG 2 cut(s) 576, 1736
EcoRV GATATC 1 cut(s) 373
EcoT14I CCWWGG 2 cut(s) 474, 1222
EcoT22I ATGCAT 1 cut(s) 810
EgeI GGCGCC 2 cut(s) 265, 1579
EheI GGCGCC 2 cut(s) 265, 1579
ErhI CCWWGG 2 cut(s) 474, 1222
Esp3I CGTCTC 2 cut(s) 236, 407
FaeI CATG 6 cut(s) 598, 799, 1051, 1226, 1321, 1707
FaiI YATR 7 cut(s) 83, 596, 797, 1049, 1224, 1319, 1705
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 3 cut(s) 597, 706, 976
FatI CATG 6 cut(s) 594, 795, 1047, 1222, 1317, 1703
FauI CCCGC 2 cut(s) 113, 300
FokI GGATG 6 cut(s) 60, 83, 444, 1325, 1388, 1792
FspBI CTAG 5 cut(s) 1344, 1491, 1556, 1731, 1809
GlaI GCGC 7 cut(s) 265, 590, 592, 660, 739, 1524, 1579
GsaI CCCAGC 4 cut(s) 154, 744, 1478, 1759
GsuI CTGGAG 1 cut(s) 1583
HaeII RGCGCY 4 cut(s) 267, 741, 1526, 1581
HgaI GACGC 3 cut(s) 499, 657, 687
HhaI GCGC 7 cut(s) 266, 591, 593, 661, 740, 1525, 1580
Hin1I GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
Hin1II CATG 6 cut(s) 598, 799, 1051, 1226, 1321, 1707
Hin6I GCGC 7 cut(s) 264, 589, 591, 659, 738, 1523, 1578
HinP1I GCGC 7 cut(s) 264, 589, 591, 659, 738, 1523, 1578
HincII GTYRAC 2 cut(s) 30, 1363
HindII GTYRAC 2 cut(s) 30, 1363
HinfI GANTC 9 cut(s) 47, 284, 311, 565, 673, 1276, 1444, 1551, 1633
Hpy166II GTNNAC 5 cut(s) 30, 438, 655, 1363, 1601
Hpy188III TCNNGA 8 cut(s) 77, 338, 454, 533, 604, 1026, 1562, 1657
Hpy8I GTNNAC 5 cut(s) 30, 438, 655, 1363, 1601
Hpy99I CGWCG 8 cut(s) 41, 116, 263, 371, 493, 673, 703, 1611
HpyCH4III ACNGT 8 cut(s) 37, 344, 383, 424, 827, 1370, 1415, 1611
HpyCH4IV ACGT 7 cut(s) 206, 229, 440, 480, 527, 731, 1454
HpySE526I ACGT 7 cut(s) 206, 229, 440, 480, 527, 731, 1454
Hsp92I GRCGYC 5 cut(s) 264, 491, 698, 1454, 1578
Hsp92II CATG 6 cut(s) 598, 799, 1051, 1226, 1321, 1707
HspAI GCGC 7 cut(s) 264, 589, 591, 659, 738, 1523, 1578
KasI GGCGCC 2 cut(s) 263, 1577
Kpn2I TCCGGA 1 cut(s) 453
Kzo9I GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
LmnI GCTCC 6 cut(s) 14, 159, 1218, 1295, 1349, 1436
Lsp1109I GCAGC 4 cut(s) 640, 1246, 1514, 1570
LweI GCATC 2 cut(s) 189, 1181
MabI ACCWGGT 1 cut(s) 576
MaeI CTAG 5 cut(s) 1344, 1491, 1556, 1731, 1809
MaeII ACGT 7 cut(s) 206, 229, 440, 480, 527, 731, 1454
MaeIII GTNAC 3 cut(s) 727, 982, 1510
MalI GATC 9 cut(s) 62, 333, 537, 572, 1209, 1230, 1383, 1519, 1560
MbiI CCGCTC 2 cut(s) 429, 645
MboI GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
MflI RGATCY 2 cut(s) 1207, 1558
MhlI GDGCHC 4 cut(s) 446, 657, 1603, 1664
MluCI AATT 4 cut(s) 84, 834, 1681, 1767
Mly113I GGCGCC 2 cut(s) 264, 1578
MlyI GAGTC 5 cut(s) 278, 305, 1453, 1560, 1642
Mph1103I ATGCAT 1 cut(s) 810
MroI TCCGGA 1 cut(s) 453
MseI TTAA 2 cut(s) 1766, 1772
MspA1I CMGCKG 4 cut(s) 68, 631, 1505, 1583
MspR9I CCNGG 6 cut(s) 506, 578, 681, 1212, 1335, 1738
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 2 cut(s) 578, 1738
MvnI CGCG 7 cut(s) 534, 591, 659, 661, 668, 1027, 1409
NarI GGCGCC 2 cut(s) 264, 1578
NciI CCSGG 4 cut(s) 506, 681, 1212, 1335
NcoI CCATGG 1 cut(s) 1222
NdeII GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
NlaIII CATG 6 cut(s) 598, 799, 1051, 1226, 1321, 1707
NmuCI GTSAC 2 cut(s) 727, 982
NruI TCGCGA 2 cut(s) 534, 1027
NsiI ATGCAT 1 cut(s) 810
NspI RCATGY 1 cut(s) 1321
PaeI GCATGC 1 cut(s) 1321
PaeR7I CTCGAG 1 cut(s) 604
PalAI GGCGCGCC 1 cut(s) 589
PaqCI CACCTGC 2 cut(s) 754, 775
PauI GCGCGC 1 cut(s) 589
PcsI WCGNNNNNNNCGW 2 cut(s) 446, 1285
PctI GAATGC 1 cut(s) 810
PfeI GAWTC 4 cut(s) 47, 565, 673, 1276
PflFI GACNNNGTC 1 cut(s) 697
Ple19I CGATCG 1 cut(s) 1384
PleI GAGTC 5 cut(s) 278, 305, 1452, 1559, 1641
PluTI GGCGCC 2 cut(s) 267, 1581
PmaCI CACGTG 2 cut(s) 207, 528
PmlI CACGTG 2 cut(s) 207, 528
PpsI GAGTC 5 cut(s) 278, 305, 1452, 1559, 1641
Ppu21I YACGTR 3 cut(s) 207, 441, 528
PpuMI RGGWCCY 1 cut(s) 1734
PshAI GACNNNNGTC 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 1734
Psp6I CCWGG 2 cut(s) 576, 1736
PspCI CACGTG 2 cut(s) 207, 528
PspFI CCCAGC 4 cut(s) 150, 740, 1474, 1755
PspGI CCWGG 2 cut(s) 576, 1736
PspPPI RGGWCCY 1 cut(s) 1734
PsrI GAACNNNNNNTAC 2 cut(s) 1150, 1182
PstI CTGCAG 1 cut(s) 786
PstNI CAGNNNCTG 1 cut(s) 183
PsuI RGATCY 2 cut(s) 1207, 1558
PsyI GACNNNGTC 1 cut(s) 697
PteI GCGCGC 1 cut(s) 589
PvuI CGATCG 1 cut(s) 1384
RruI TCGCGA 2 cut(s) 534, 1027
RsaI GTAC 2 cut(s) 503, 1168
RsaNI GTAC 2 cut(s) 502, 1167
SaqAI TTAA 2 cut(s) 1766, 1772
Sau3AI GATC 9 cut(s) 60, 331, 535, 570, 1207, 1228, 1381, 1517, 1558
SbfI CCTGCAGG 1 cut(s) 786
SchI GAGTC 5 cut(s) 278, 305, 1453, 1560, 1642
ScrFI CCNGG 6 cut(s) 506, 578, 681, 1212, 1335, 1738
SdaI CCTGCAGG 1 cut(s) 786
SduI GDGCHC 4 cut(s) 446, 657, 1603, 1664
SexAI ACCWGGT 1 cut(s) 576
SfaNI GCATC 2 cut(s) 189, 1181
SfcI CTRYAG 1 cut(s) 782
SfoI GGCGCC 2 cut(s) 265, 1579
Sfr274I CTCGAG 1 cut(s) 604
SgrAI CRCCGGYG 1 cut(s) 493
SgsI GGCGCGCC 1 cut(s) 589
SinI GGWCC 4 cut(s) 469, 1010, 1337, 1734
SlaI CTCGAG 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 604
SmoI CTYRAG 1 cut(s) 604
SpeI ACTAGT 1 cut(s) 1343
SphI GCATGC 1 cut(s) 1321
Sse8387I CCTGCAGG 1 cut(s) 786
Sse9I AATT 4 cut(s) 84, 834, 1681, 1767
SspDI GGCGCC 2 cut(s) 263, 1577
SspMI CTAG 5 cut(s) 1344, 1491, 1556, 1731, 1809
StyD4I CCNGG 6 cut(s) 504, 576, 679, 1210, 1333, 1736
StyI CCWWGG 2 cut(s) 474, 1222
TaaI ACNGT 8 cut(s) 37, 344, 383, 424, 827, 1370, 1415, 1611
TaiI ACGT 7 cut(s) 209, 232, 443, 483, 530, 734, 1457
TaqI TCGA 9 cut(s) 39, 59, 111, 369, 573, 605, 671, 1549, 1636
TasI AATT 4 cut(s) 84, 834, 1681, 1767
TauI GCSGC 9 cut(s) 137, 320, 323, 326, 329, 634, 738, 1508, 1583
TfiI GAWTC 4 cut(s) 47, 565, 673, 1276
Tru1I TTAA 2 cut(s) 1766, 1772
Tru9I TTAA 2 cut(s) 1766, 1772
TscAI CASTG 3 cut(s) 641, 719, 1240
TseFI GTSAC 2 cut(s) 727, 982
TseI GCWGC 4 cut(s) 628, 1259, 1502, 1583
Tsp45I GTSAC 2 cut(s) 727, 982
TspDTI ATGAA 3 cut(s) 57, 611, 1036
TspGWI ACGGA 5 cut(s) 15, 431, 816, 921, 1605
TspRI CASTG 3 cut(s) 641, 719, 1240
Tth111I GACNNNGTC 1 cut(s) 697
VneI GTGCAC 2 cut(s) 653, 1599
VpaK11BI GGWCC 4 cut(s) 469, 1010, 1337, 1734
XagI CCTNNNNNAGG 1 cut(s) 1713
XceI RCATGY 1 cut(s) 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1160
XhoI CTCGAG 1 cut(s) 604
XspI CTAG 5 cut(s) 1344, 1491, 1556, 1731, 1809
ZraI GACGTC 1 cut(s) 1455
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.