MD12G1096200.v1.1

Ubiquitin-associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015963
Physical Location & Seq
Forward (+)
13435433 .. 13447004
11572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1096200.v1.1.491

Sequence Viewer

Length: 876 bp
ATGAACGGCGGCCCATCTGGTTTCCACAATGCTCCTGTTACCAGAGCCTTCGTCATCGCCAGCGGTCTCTTCACCGTCTTCTTCGGGATCCAAGGCCGTTCTAGCAAGCTTGGATTGTCATATCTGGACATATTCGGGAAGTTTCGCCTTTGGAAGTTAATTGTGTCAATTTTTGCCTTTTCATCTACACCAGAACTGATGTTTGGGCTGTACCTACTATACTACTTCAGGGTCTTTGAGAGACAGATAGGTTCCAATAAGTACTCGGTGTTCATTTTGTACACTGTAACAGTATCATTACTCTTTGAGATCCTTGCTCTGGCAATCCTTAAAGATCCAGCAGTAAACCTAGTGACTTCAGGACCTTATGGTCTTATATTTGCATCTTTTGTACCCTTTTTCTTTGATATTCCAGTTTCAACACGCTTTCGTGTATTTGGTGTTCGCTTCTCGGATAAGTCTTTCATATATCTAGCCGGCCTACAGCTTCTTTTATCATCCTGGAAAAGATCAATCTTACCAGGGATATTTGGAATCATTTGTGGTTCCTTATATCATCTGAATGTCTTCTGTATCCGCAAAGCAAAGTTCCCAGAATTCATTGCTTCTTTTCTTTCACGGCTTTCTTGGCCATCAATGGGGACTCCGCCAGCAGCACCATCTAGAAATGTTGTGGGAAATGCACCACCATTCACAGCTCCGCAAGTAGAGAGAAACATTCCCTCTTCCATGTCTGCAACAGAGCCAACAGAGGACTCCATTGCTACTCTGGTTTCTATGGGCTTTGATAGGAACTCTGCTAGACAGGCACTAGTGCAGGCTAGAAATGACATCACTGTAGCAACAAACATCCTCCTAGAAGCACAGGCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DER1 Rhomboid UBA

Protein Analysis

292

Amino Acids

32.21

Weight (kDa)

9.82

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 49 - 187 4.9e-06 Rhomboid domain
UBA PF00627 250 - 285 6.8e-10 UBA/TS-N domain
UBA_7 PF22562 251 - 289 1e-06 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56740
fragaria_vesca FvH4_6g25340
malus_domestica MD12G1096200.v1.1 MD14G1089500.v1.1
prunus_persica Prupe.7G034400_v2.0.a1
pyrus_communis pycom12g08520 pycom14g07930
rosa_chinensis RchiOBHm_Chr2g0125741
rosa_laevigata RLG00000018878
rosa_multiflora Rmu_ssc0000055.1_g000017
rosa_roxburghii Rroxscaffold_2G00117780
rosa_rugosa Rorug02G0258800
rosa_samantha Rh2BG325000 Rh2CG306900
rosa_wichuraiana Rw2G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 369
AciI CCGC 5 cut(s) 9, 63, 577, 647, 701
AclWI GGATC 4 cut(s) 82, 95, 304, 329
AcoI YGGCCR 1 cut(s) 629
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 2 cut(s) 211, 342
AfaI GTAC 4 cut(s) 212, 263, 281, 393
AfiI CCNNNNNNNGG 2 cut(s) 319, 638
AgsI TTSAA 1 cut(s) 420
AhlI ACTAGT 1 cut(s) 811
AjnI CCWGG 2 cut(s) 500, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AluBI AGCT 3 cut(s) 109, 487, 698
AluI AGCT 3 cut(s) 109, 487, 698
Alw26I GTCTC 2 cut(s) 71, 235
AlwI GGATC 4 cut(s) 82, 95, 304, 329
AoxI GGCC 5 cut(s) 10, 94, 478, 629, 867
ApeKI GCWGC 1 cut(s) 653
ApoI RAATTY 1 cut(s) 596
Asp700I GAANNNNTTC 1 cut(s) 566
AspS9I GGNCC 3 cut(s) 11, 362, 868
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 1 cut(s) 362
BalI TGGCCA 1 cut(s) 631
BamHI GGATCC 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 70, 559
BbvI GCAGC 1 cut(s) 665
BccI CCATC 3 cut(s) 22, 640, 667
BceAI ACGGC 3 cut(s) 22, 81, 635
BciT130I CCWGG 2 cut(s) 502, 522
BciVI GTATCC 1 cut(s) 584
BcoDI GTCTC 2 cut(s) 71, 235
BcuI ACTAGT 1 cut(s) 811
BfaI CTAG 8 cut(s) 102, 350, 473, 663, 801, 812, 822, 857
BfmI CTRYAG 2 cut(s) 482, 837
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 2 cut(s) 10, 654
BlsI GCNGC 2 cut(s) 11, 655
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 2 cut(s) 502, 522
Bme18I GGWCC 1 cut(s) 362
BmgT120I GGNCC 3 cut(s) 11, 362, 868
BmiI GGNNCC 3 cut(s) 89, 253, 547
BmrFI CCNGG 2 cut(s) 502, 522
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 2 cut(s) 70, 559
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 91, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 319, 638
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 2 cut(s) 600, 759
BseBI CCWGG 2 cut(s) 502, 522
BseDI CCNNGG 2 cut(s) 91, 521
BseGI GGATG 2 cut(s) 497, 849
BseLI CCNNNNNNNGG 2 cut(s) 319, 638
BseMI GCAATG 2 cut(s) 600, 759
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 665
BsgI GTGCAG 1 cut(s) 836
BshFI GGCC 5 cut(s) 12, 96, 480, 631, 869
BsiSI CCGG 1 cut(s) 477
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 2 cut(s) 319, 638
BsmAI GTCTC 2 cut(s) 71, 235
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 5 cut(s) 12, 96, 480, 631, 869
Bso31I GGTCTC 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 279
Bsp143I GATC 4 cut(s) 87, 309, 334, 509
BspACI CCGC 5 cut(s) 9, 63, 577, 647, 701
BspANI GGCC 5 cut(s) 12, 96, 480, 631, 869
BspLI GGNNCC 3 cut(s) 89, 253, 547
BspPI GGATC 4 cut(s) 82, 95, 304, 329
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 2 cut(s) 600, 759
BsrFI RCCGGY 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 279
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 91, 521
BssMI GATC 4 cut(s) 87, 309, 334, 509
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 2 cut(s) 502, 522
Bst4CI ACNGT 4 cut(s) 76, 286, 292, 838
Bst6I CTCTTC 2 cut(s) 74, 730
BstAUI TGTACA 1 cut(s) 279
BstC8I GCNNGC 5 cut(s) 61, 107, 478, 651, 819
BstF5I GGATG 2 cut(s) 497, 849
BstKTI GATC 4 cut(s) 90, 312, 337, 512
BstMAI GTCTC 2 cut(s) 71, 235
BstMBI GATC 4 cut(s) 87, 309, 334, 509
BstMWI GCNNNNNNNGC 3 cut(s) 102, 628, 806
BstNI CCWGG 2 cut(s) 502, 522
BstSCI CCNGG 2 cut(s) 500, 520
BstSFI CTRYAG 2 cut(s) 482, 837
BstV1I GCAGC 1 cut(s) 665
BstV2I GAAGAC 2 cut(s) 70, 559
BstX2I RGATCY 3 cut(s) 87, 309, 334
BstYI RGATCY 3 cut(s) 87, 309, 334
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 5 cut(s) 12, 96, 480, 631, 869
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 2 cut(s) 497, 849
BtsIMutI CAGTG 2 cut(s) 282, 834
Cac8I GCNNGC 5 cut(s) 61, 107, 478, 651, 819
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 3 cut(s) 11, 362, 868
Csp6I GTAC 4 cut(s) 211, 262, 280, 392
CviAII CATG 1 cut(s) 730
CviQI GTAC 4 cut(s) 211, 262, 280, 392
DpnI GATC 4 cut(s) 89, 311, 336, 511
DpnII GATC 4 cut(s) 87, 309, 334, 509
DrdI GACNNNNNNGTC 1 cut(s) 369
DseDI GACNNNNNNGTC 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 629
Eam1104I CTCTTC 2 cut(s) 74, 730
EarI CTCTTC 2 cut(s) 74, 730
EciI GGCGGA 1 cut(s) 636
Eco130I CCWWGG 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 362
Eco57I CTGAAG 2 cut(s) 211, 342
EcoO109I RGGNCCY 1 cut(s) 362
EcoRI GAATTC 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 500, 520
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 1 cut(s) 733
FaqI GGGAC 1 cut(s) 655
FatI CATG 1 cut(s) 729
Fnu4HI GCNGC 2 cut(s) 10, 654
FokI GGATG 2 cut(s) 484, 836
Fsp4HI GCNGC 2 cut(s) 10, 654
FspBI CTAG 8 cut(s) 102, 350, 473, 663, 801, 812, 822, 857
GluI GCNGC 2 cut(s) 10, 654
HaeIII GGCC 5 cut(s) 12, 96, 480, 631, 869
HapII CCGG 1 cut(s) 477
Hin1II CATG 1 cut(s) 733
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 3 cut(s) 534, 643, 755
HpaII CCGG 1 cut(s) 477
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 282, 346
Hpy188I TCNGA 2 cut(s) 454, 561
Hpy188III TCNNGA 5 cut(s) 85, 125, 136, 360, 663
Hpy8I GTNNAC 2 cut(s) 282, 346
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 4 cut(s) 76, 286, 292, 838
HpyCH4V TGCA 4 cut(s) 383, 683, 737, 817
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 628, 806
Hsp92II CATG 1 cut(s) 733
KroI GCCGGC 1 cut(s) 476
KroNI GCCGGC 1 cut(s) 478
Kzo9I GATC 4 cut(s) 87, 309, 334, 509
LmnI GCTCC 2 cut(s) 37, 703
Lsp1109I GCAGC 1 cut(s) 665
LweI GCATC 1 cut(s) 392
MaeI CTAG 8 cut(s) 102, 350, 473, 663, 801, 812, 822, 857
MaeIII GTNAC 3 cut(s) 37, 286, 352
MalI GATC 4 cut(s) 89, 311, 336, 511
MboI GATC 4 cut(s) 87, 309, 334, 509
MboII GAAGA 5 cut(s) 61, 70, 73, 559, 717
MflI RGATCY 3 cut(s) 87, 309, 334
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 3 cut(s) 159, 168, 596
MluNI TGGCCA 1 cut(s) 631
MlyI GAGTC 2 cut(s) 637, 749
MnlI CCTC 3 cut(s) 733, 745, 863
Mox20I TGGCCA 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 476
MroXI GAANNNNTTC 1 cut(s) 566
MscI TGGCCA 1 cut(s) 631
MseI TTAA 2 cut(s) 158, 330
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 631
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 2 cut(s) 502, 522
MvaI CCWGG 2 cut(s) 502, 522
MwoI GCNNNNNNNGC 3 cut(s) 102, 628, 806
NaeI GCCGGC 1 cut(s) 478
NdeII GATC 4 cut(s) 87, 309, 334, 509
NgoMIV GCCGGC 1 cut(s) 476
NlaIII CATG 1 cut(s) 733
NlaIV GGNNCC 3 cut(s) 89, 253, 547
NmuCI GTSAC 1 cut(s) 352
PdiI GCCGGC 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 1 cut(s) 534
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 2 cut(s) 11, 655
PleI GAGTC 2 cut(s) 637, 749
PpsI GAGTC 2 cut(s) 637, 749
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 500, 520
PspGI CCWGG 2 cut(s) 500, 520
PspN4I GGNNCC 3 cut(s) 89, 253, 547
PspPI GGNCC 3 cut(s) 11, 362, 868
PspPPI RGGWCCY 1 cut(s) 362
PsrI GAACNNNNNNTAC 4 cut(s) 254, 286, 426, 458
PsuI RGATCY 3 cut(s) 87, 309, 334
RsaI GTAC 4 cut(s) 212, 263, 281, 393
RsaNI GTAC 4 cut(s) 211, 262, 280, 392
RseI CAYNNNNRTG 1 cut(s) 561
SaqAI TTAA 2 cut(s) 158, 330
SatI GCNGC 2 cut(s) 10, 654
Sau3AI GATC 4 cut(s) 87, 309, 334, 509
Sau96I GGNCC 3 cut(s) 11, 362, 868
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 2 cut(s) 637, 749
ScrFI CCNGG 2 cut(s) 502, 522
SetI ASST 7 cut(s) 111, 216, 253, 351, 367, 489, 700
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 2 cut(s) 482, 837
SinI GGWCC 1 cut(s) 362
SmiMI CAYNNNNRTG 1 cut(s) 561
SpeI ACTAGT 1 cut(s) 811
Sse9I AATT 3 cut(s) 159, 168, 596
SsiI CCGC 5 cut(s) 9, 63, 577, 647, 701
SspMI CTAG 8 cut(s) 102, 350, 473, 663, 801, 812, 822, 857
StyD4I CCNGG 2 cut(s) 500, 520
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 4 cut(s) 76, 286, 292, 838
TasI AATT 3 cut(s) 159, 168, 596
TatI WGTACW 2 cut(s) 261, 279
TauI GCSGC 1 cut(s) 12
TfiI GAWTC 1 cut(s) 534
Tru1I TTAA 2 cut(s) 158, 330
Tru9I TTAA 2 cut(s) 158, 330
TscAI CASTG 2 cut(s) 289, 841
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 5 cut(s) 17, 171, 262, 454, 589
TspRI CASTG 2 cut(s) 289, 841
VpaK11BI GGWCC 1 cut(s) 362
XapI RAATTY 1 cut(s) 596
XbaI TCTAGA 1 cut(s) 662
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 8 cut(s) 102, 350, 473, 663, 801, 812, 822, 857
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.