Rorug02G0258800

Ubiquitin-associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015963
Physical Location & Seq
Reverse (-)
27228892 .. 27239056
10165 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0258800.1

Sequence Viewer

Length: 4623 bp
ATGGCGGACGACGAGCAACCGATCGTGACAGGGAGGCGGCGGAGCCGAGGAGCCGAGGCGTCGGCGCGGTCCAGCGCCCTGGAGCGCCTCCGAGCTCTCCGGCAAGGCGGCCGCCGCTCCGAAAACGCCGACGACCGGTTCCAGATCAAGATGGAGAAGCCGATCTACGACACGGTCGACGAGGACGAGTACGACGCCATAATTGCCAAGCGGCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGACCCGCCGCGGGCTTCCGACGTCGTCGGATGAATCGGACGGCGATATGAGGCCCAGGAGGAGGAAGAAGGTGGAGAAGAAGGAGAAGGAGAAGGAGCCGCGGTCCAAAAAGCCCAATTCGTCGCTTACGGCGGCGGCGGCGATGATGGGGAAGCAGAGGCTTTCGTCGATGTTCACGTCGTCCGTGTTTAATAAAAGTAGGGACGGTGACAAGGCCAAAGGATTGTCTTGTGATAGCATTGTGGATGATGTGATTGCCGAGTTTGCGCCGGACGAGGCTGATAGAGAGAGACGGAGGAGGGCACAGCCGGTGAGGAGTTTCGTTCCGATTACCGGTGTTAAGAGTGAGAGAGTGAGTAATGGGGGTGTTGAGTTATCAAGGAGGCTTGAATTGGATAGAATTGTTGCAAATGGTAGTGGTTCTGTGCATAGTAAGGAAATGGATAAAGATTCTGAGGGCAAGAAGGAGTTGGATTTCAGAAATTGTCAGCCCCAAATTGAGCAATTCCATGATTTTTTGGAGAATAGTGAGGTGCCCGAGGAGAAGAAGACTGAGGATTCTGCTGAGGTGCAGGCGGAGCCTGTGGTGAAGAAGGAAGAGGTTTTCACTTTGAATGCAAAGATTAAGGAAGATGAGGATCCGGCATTGAGTGCTATGGCAGGATGGAAGGCCGCGATGACTGGTGGGGCAGGGGTTACTGGTGGCGGCGGCAATGTGGAGGTCAAAAGTGACTCAATTAGTGAAGGGAGTTCGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAATATGGGCACTGTTTATCTATTTGGGAAGGTTAAAGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTAAAGAACATGCAAAGATGTGTCTATGCAATTCCGGACAGTCCTTTATTTCAAACTGATGAAATGATGAAGCTCGAAAAGGATGCCGAGGACTCAAGGATTTCACCCACAGATTTTCGTAAGAAGCTGCATGATGCGGCTTCAGAATTAAAGAATGAATTAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGGCACCTGTCAAGAGGAACTATGCTTTTGAGCGATCTGACATACCTGCAGGGGAGAATTATGTACTGAAGATCAATTATCCATTCCGGGATCCACCACTATCAGCAGATCTAAAAGGAGAAACTTTTTGTGCTCTTCTAGGAACTCATAGCAGCGCGTTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCCATCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTGAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACAAAGAAAACTGTAGATATTCCTCAAGTGGTTGTTACTGCAATAAGTTTGAAAACTATTATCAATGAGAAGCAGAATGTTAACGAAATTGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGTGGCATATTTCCTATGGGGTTTACTAAAGTGGCTGCAGATAAGAATTCTAAAGCCGGATCAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTAAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTTGTTGGACATAATATATCTGGATTTGATCTGGATGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTCGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTCAGCTATTCCTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCGCGTGATATTCCTAGAATGTTCCAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCATGGTTGTCTATGGGACTCATGTTTCATTTGAGTGCTCTTCCCCTCACACGTCAGCTGACTAATATAAGTGGTAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTAGAATATCTCTTACTGCATGCTTTTCATGCAAAAAAGTATATTGTTCCAGACAAGATTCCTCATCTGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGTGTTGAGGAAAAAGCTGCTGATGAGTTGGATGTAAATGATGTAAATGTTGATAATGATGCACAGAGTGGTCATGGAAAAGGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTATTGGAGCCAAAAAAAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATACAATATATGCTTCACAACTGTTGAAAGAAATTCAGAAGGATTGGTTTCTCATTTACCTTCTAGTCAAAGACCTGGACTTTTACCTGAGTTGTTAAAAAATTTAGTTGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAGCTTGACATTCAACAGCAAGCTCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAACTCAAGATTTTATGCAAAGCCACTGGCAGAGCTTATAACACTACGGGGCAGGGAGATCCTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTAATCTATGGTGATACGGACTCAATAATGATTTATAGTGGGCTGGATGATAGTGCGAAAGCAAAAGCAATTGCTGTGAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGCTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCGGCTGTGAAGGAGCTAACGAAAAACGAGAAAAAATACGAGGTTATCGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGTTTACTTTCAAAGGAACTGGGAGATTTCTGCTTAAGTCAAATTTTGTCAGGAGGGTCATCTGAAGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTGCCCTCGAGAAATATATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAGTGGTTACTCTACTGGTTGCTCTGTTGGTGATACAGTCCCTTATATTATTTGCTGTGAGCAGGGCACAAATTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAGATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCTGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGCCCCCAACGCTTGGCTGATTGTTTAGGGATTGACTCATCCAAGTTCAAATCGAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTGTTGACGATGAAGAGAGATATCGGGATTGCGAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTCCATTGCCAAGTCAATCAATGAAAAGCCAACAAGGCCACAAGGAGAAGAATCTTCCAGCAATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCCAATCAGGTCAAAAGGCAAGCAGAGGGTTTTGTTTCAATGTACTACAGGGGCATAATGACGTGTGATGATGAAACTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATGCTGAGAGAGGAACAGTCTGTCCAAACTATCCTCACTGCAATGGGCGTCTTATAAGAAAGTACACGGAAGCTGATTTGTACAAACAGCTCTCATTTTTCTGCCATGTCTTGGATACAGTACGCTGCGTAGAAAAGATGGAACCTAAGACTAGACTGCCGGTAGAGTTAGAGTTGGCCAAAATTCGCCCAATTGTGACTTTGGCTGCCTCAACAGTTGAAAGGATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTACAAGATCTTTTTGTTGCGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DER1 Rhomboid UBA

Protein Analysis

1540

Amino Acids

172.4

Weight (kDa)

7.93

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DNA_pol_alpha_N PF12254 27 - 96 9.6e-20 DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1 PF03104 511 - 765 2.2e-26 DNA polymerase family B, exonuclease domain
DNA_pol_B PF00136 831 - 1293 9.6e-126 DNA polymerase family B
zf-DNA_Pol PF08996 1331 - 1537 1.3e-43 DNA Polymerase alpha zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56740
fragaria_vesca FvH4_6g25340
malus_domestica MD12G1096200.v1.1 MD14G1089500.v1.1
prunus_persica Prupe.7G034400_v2.0.a1
pyrus_communis pycom12g08520 pycom14g07930
rosa_chinensis RchiOBHm_Chr2g0125741
rosa_laevigata RLG00000018878
rosa_multiflora Rmu_ssc0000055.1_g000017
rosa_roxburghii Rroxscaffold_2G00117780
rosa_rugosa Rorug02G0258800
rosa_samantha Rh2BG325000 Rh2CG306900
rosa_wichuraiana Rw2G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 3104, 4394
AatII GACGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 1438
Acc65I GGTACC 2 cut(s) 258, 3884
AccB1I GGYRCC 4 cut(s) 258, 820, 1387, 3884
AccB7I CCANNNNNTGG 2 cut(s) 3901, 4450
AccBSI CCGCTC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 177
AccII CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
AccIII TCCGGA 1 cut(s) 1216
AclWI GGATC 9 cut(s) 920, 933, 1368, 1469, 1482, 1963, 2216, 3118, 3150
AcoI YGGCCR 2 cut(s) 109, 4515
AcsI RAATTY 9 cut(s) 1600, 1643, 1942, 2863, 2932, 3471, 3736, 3943, 4521
AcuI CTGAAG 5 cut(s) 1308, 1472, 2618, 3044, 3513
AcyI GRCGYC 4 cut(s) 59, 195, 308, 4387
AdeI CACNNNGTG 2 cut(s) 2345, 2702
AflII CTTAAG 1 cut(s) 3463
AflIII ACRYGT 3 cut(s) 2462, 3637, 4280
AgeI ACCGGT 2 cut(s) 135, 620
AjiI CACGTC 3 cut(s) 465, 2465, 4281
AjnI CCWGG 5 cut(s) 78, 341, 1855, 2203, 2905
AleI CACNNNNGTG 1 cut(s) 1829
AloI GAACNNNNNNTCC 2 cut(s) 4345, 4377
Alw21I GWGCWC 4 cut(s) 97, 1519, 2095, 2452
Alw26I GTCTC 7 cut(s) 571, 1687, 1789, 2399, 2990, 4209, 4322
AlwI GGATC 9 cut(s) 920, 933, 1368, 1469, 1482, 1963, 2216, 3118, 3150
Ama87I CYCGRG 2 cut(s) 824, 3566
Aor13HI TCCGGA 1 cut(s) 1216
ApoI RAATTY 9 cut(s) 1600, 1643, 1942, 2863, 2932, 3471, 3736, 3943, 4521
AsiGI ACCGGT 2 cut(s) 135, 620
Asp700I GAANNNNTTC 3 cut(s) 3510, 3967, 4563
Asp718I GGTACC 2 cut(s) 258, 3884
AspA2I CCTAGG 1 cut(s) 253
AspLEI GCGC 8 cut(s) 67, 77, 87, 216, 556, 1541, 3652, 4164
AsuC2I CCSGG 2 cut(s) 1472, 3754
AvaI CYCGRG 2 cut(s) 824, 3566
AvaII GGWCC 8 cut(s) 69, 288, 390, 1109, 1577, 2124, 2726, 4570
AvrII CCTAGG 1 cut(s) 253
BaeGI GKGCMC 5 cut(s) 592, 825, 1124, 2524, 3734
BalI TGGCCA 1 cut(s) 4517
BamHI GGATCC 2 cut(s) 925, 1474
BanI GGYRCC 4 cut(s) 258, 820, 1387, 3884
BanII GRGCYC 2 cut(s) 97, 2095
BarI GAAGNNNNNNTAC 2 cut(s) 149, 181
BbsI GAAGAC 1 cut(s) 842
Bbv12I GWGCWC 4 cut(s) 97, 1519, 2095, 2452
BbvCI CCTCAGC 1 cut(s) 852
BceAI ACGGC 5 cut(s) 265, 286, 343, 432, 3319
BcgI CGANNNNNNTGC 4 cut(s) 1247, 1281, 2393, 2427
BciT130I CCWGG 5 cut(s) 80, 343, 1857, 2205, 2907
BciVI GTATCC 3 cut(s) 2596, 4447, 4584
BcnI CCSGG 2 cut(s) 1472, 3754
BcoDI GTCTC 7 cut(s) 571, 1687, 1789, 2399, 2990, 4209, 4322
BfaI CTAG 7 cut(s) 254, 1523, 2355, 2895, 3771, 4044, 4491
BfmI CTRYAG 4 cut(s) 1431, 1704, 1932, 4264
BfoI RGCGCY 2 cut(s) 78, 88
BfrI CTTAAG 1 cut(s) 3463
BfuAI ACCTGC 1 cut(s) 1438
BfuI GTATCC 3 cut(s) 2596, 4447, 4584
BglI GCCNNNNNGGC 1 cut(s) 4117
BglII AGATCT 2 cut(s) 1492, 4603
BlnI CCTAGG 1 cut(s) 253
BlpI GCTNAGC 1 cut(s) 1865
BmcAI AGTACT 1 cut(s) 3136
Bme1390I CCNGG 7 cut(s) 80, 343, 1472, 1857, 2205, 2907, 3754
Bme18I GGWCC 8 cut(s) 69, 288, 390, 1109, 1577, 2124, 2726, 4570
BmeT110I CYCGRG 2 cut(s) 824, 3566
BmgBI CACGTC 3 cut(s) 465, 2465, 4281
BmrFI CCNGG 7 cut(s) 80, 343, 1472, 1857, 2205, 2907, 3754
BmrI ACTGGG 1 cut(s) 3458
BmuI ACTGGG 1 cut(s) 3458
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 3 cut(s) 101, 2214, 3448
Bpu10I CCTNAGC 3 cut(s) 852, 2094, 2160
Bpu1102I GCTNAGC 1 cut(s) 1865
BpuEI CTTGAG 7 cut(s) 1261, 1701, 1987, 2126, 2972, 3055, 3314
BpuMI CCSGG 2 cut(s) 1472, 3754
BsaBI GATNNNNATC 2 cut(s) 924, 1047
BsaHI GRCGYC 4 cut(s) 59, 195, 308, 4387
BsaI GGTCTC 2 cut(s) 1687, 2990
BsaWI WCCGGW 4 cut(s) 135, 620, 1216, 2325
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bse118I RCCGGY 4 cut(s) 135, 595, 620, 4498
Bse1I ACTGG 7 cut(s) 290, 973, 991, 3096, 3431, 3453, 3685
Bse3DI GCAATG 4 cut(s) 1006, 2220, 4086, 4387
Bse8I GATNNNNATC 2 cut(s) 924, 1047
BseAI TCCGGA 1 cut(s) 1216
BseBI CCWGG 5 cut(s) 80, 343, 1857, 2205, 2907
BseJI GATNNNNATC 2 cut(s) 924, 1047
BseMI GCAATG 4 cut(s) 1006, 2220, 4086, 4387
BseNI ACTGG 7 cut(s) 290, 973, 991, 3096, 3431, 3453, 3685
BseRI GAGGAG 7 cut(s) 63, 233, 361, 598, 616, 842, 2962
BseSI GKGCMC 5 cut(s) 592, 825, 1124, 2524, 3734
BseX3I CGGCCG 1 cut(s) 109
BseYI CCCAGC 2 cut(s) 1615, 2314
BsgI GTGCAG 2 cut(s) 878, 4174
Bsh1236I CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
Bsh1285I CGRYCG 4 cut(s) 24, 112, 136, 177
BshNI GGYRCC 4 cut(s) 258, 820, 1387, 3884
BshTI ACCGGT 2 cut(s) 135, 620
BsiEI CGRYCG 4 cut(s) 24, 112, 136, 177
BsiHKAI GWGCWC 4 cut(s) 97, 1519, 2095, 2452
BsiHKCI CYCGRG 2 cut(s) 824, 3566
BslFI GGGAC 6 cut(s) 242, 503, 2442, 3689, 3820, 4583
BsmAI GTCTC 7 cut(s) 571, 1687, 1789, 2399, 2990, 4209, 4322
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 6 cut(s) 242, 503, 2442, 3689, 3820, 4583
BsmI GAATGC 4 cut(s) 907, 1666, 2980, 3324
Bso31I GGTCTC 2 cut(s) 1687, 2990
BsoBI CYCGRG 2 cut(s) 824, 3566
Bsp1286I GDGCHC 9 cut(s) 97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
Bsp13I TCCGGA 1 cut(s) 1216
Bsp1407I TGTACA 2 cut(s) 3309, 4419
Bsp1720I GCTNAGC 1 cut(s) 1865
BspEI TCCGGA 1 cut(s) 1216
BspFNI CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
BspMAI CTGCAG 2 cut(s) 1435, 1936
BspMI ACCTGC 1 cut(s) 1438
BspPI GGATC 9 cut(s) 920, 933, 1368, 1469, 1482, 1963, 2216, 3118, 3150
BspQI GCTCTTC 2 cut(s) 1524, 2457
BspT107I GGYRCC 4 cut(s) 258, 820, 1387, 3884
BspTI CTTAAG 1 cut(s) 3463
BspTNI GGTCTC 2 cut(s) 1687, 2990
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 4 cut(s) 1006, 2220, 4086, 4387
BsrFI RCCGGY 4 cut(s) 135, 595, 620, 4498
BsrGI TGTACA 2 cut(s) 3309, 4419
BsrI ACTGG 7 cut(s) 290, 973, 991, 3096, 3431, 3453, 3685
BssAI RCCGGY 4 cut(s) 135, 595, 620, 4498
BssNI GRCGYC 4 cut(s) 59, 195, 308, 4387
BssT1I CCWWGG 2 cut(s) 253, 2509
Bst2UI CCWGG 5 cut(s) 80, 343, 1857, 2205, 2907
Bst6I CTCTTC 7 cut(s) 273, 879, 1092, 1524, 1844, 2457, 3996
BstACI GRCGYC 4 cut(s) 59, 195, 308, 4387
BstAFI CTTAAG 1 cut(s) 3463
BstAPI GCANNNNNTGC 1 cut(s) 938
BstAUI TGTACA 2 cut(s) 3309, 4419
BstC8I GCNNGC 6 cut(s) 299, 861, 2166, 2550, 3018, 4239
BstDSI CCRYGG 2 cut(s) 295, 386
BstFNI CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
BstH2I RGCGCY 2 cut(s) 78, 88
BstHHI GCGC 8 cut(s) 67, 77, 87, 216, 556, 1541, 3652, 4164
BstMAI GTCTC 7 cut(s) 571, 1687, 1789, 2399, 2990, 4209, 4322
BstMCI CGRYCG 4 cut(s) 24, 112, 136, 177
BstNI CCWGG 5 cut(s) 80, 343, 1857, 2205, 2907
BstNSI RCATGY 6 cut(s) 1084, 1195, 2122, 2552, 3641, 4041
BstSCI CCNGG 7 cut(s) 78, 341, 1470, 1855, 2203, 2905, 3752
BstSFI CTRYAG 4 cut(s) 1431, 1704, 1932, 4264
BstSLI GKGCMC 5 cut(s) 592, 825, 1124, 2524, 3734
BstUI CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
BstV2I GAAGAC 1 cut(s) 842
BstX2I RGATCY 7 cut(s) 925, 1360, 1474, 1492, 3123, 3142, 4603
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 7 cut(s) 925, 1360, 1474, 1492, 3123, 3142, 4603
BstZI CGGCCG 1 cut(s) 109
BsuI GTATCC 3 cut(s) 2596, 4447, 4584
BtgI CCRYGG 2 cut(s) 295, 386
BtgZI GCGATG 2 cut(s) 443, 977
BtrI CACGTC 3 cut(s) 465, 2465, 4281
BtsI GCAGTG 3 cut(s) 1067, 1987, 4375
BtsIMutI CAGTG 5 cut(s) 1067, 1122, 1987, 3089, 4375
BveI ACCTGC 1 cut(s) 1438
Cac8I GCNNGC 6 cut(s) 299, 861, 2166, 2550, 3018, 4239
CciNI GCGGCCGC 1 cut(s) 109
CfoI GCGC 8 cut(s) 67, 77, 87, 216, 556, 1541, 3652, 4164
Cfr10I RCCGGY 4 cut(s) 135, 595, 620, 4498
Cfr42I CCGCGG 2 cut(s) 298, 389
CseI GACGC 3 cut(s) 48, 203, 4376
CspAI ACCGGT 2 cut(s) 135, 620
DraIII CACNNNGTG 2 cut(s) 2345, 2702
EaeI YGGCCR 2 cut(s) 109, 4515
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 7 cut(s) 273, 879, 1092, 1524, 1844, 2457, 3996
EarI CTCTTC 7 cut(s) 273, 879, 1092, 1524, 1844, 2457, 3996
EciI GGCGGA 3 cut(s) 20, 55, 878
Ecl136II GAGCTC 2 cut(s) 95, 2093
EclXI CGGCCG 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 253, 2509
Eco24I GRGCYC 2 cut(s) 97, 2095
Eco31I GGTCTC 2 cut(s) 1687, 2990
Eco32I GATATC 1 cut(s) 4010
Eco47I GGWCC 8 cut(s) 69, 288, 390, 1109, 1577, 2124, 2726, 4570
Eco52I CGGCCG 1 cut(s) 109
Eco53kI GAGCTC 2 cut(s) 95, 2093
Eco57I CTGAAG 5 cut(s) 1308, 1472, 2618, 3044, 3513
Eco88I CYCGRG 2 cut(s) 824, 3566
EcoICRI GAGCTC 2 cut(s) 95, 2093
EcoO109I RGGNCCY 3 cut(s) 1577, 2726, 3403
EcoRI GAATTC 2 cut(s) 1942, 3943
EcoRII CCWGG 5 cut(s) 78, 341, 1855, 2203, 2905
EcoRV GATATC 1 cut(s) 4010
EcoT14I CCWWGG 2 cut(s) 253, 2509
EcoT22I ATGCAT 2 cut(s) 2416, 2980
EcoT38I GRGCYC 2 cut(s) 97, 2095
ErhI CCWWGG 2 cut(s) 253, 2509
Esp3I CGTCTC 1 cut(s) 571
FaqI GGGAC 6 cut(s) 242, 503, 2442, 3689, 3820, 4583
FauI CCCGC 2 cut(s) 290, 299
FblI GTMKAC 1 cut(s) 177
FriOI GRGCYC 2 cut(s) 97, 2095
FspBI CTAG 7 cut(s) 254, 1523, 2355, 2895, 3771, 4044, 4491
GlaI GCGC 8 cut(s) 66, 76, 86, 215, 555, 1540, 3651, 4163
GsaI CCCAGC 2 cut(s) 1619, 2318
GsuI CTGGAG 3 cut(s) 101, 2214, 3448
HaeII RGCGCY 2 cut(s) 78, 88
HgaI GACGC 3 cut(s) 48, 203, 4376
HhaI GCGC 8 cut(s) 67, 77, 87, 216, 556, 1541, 3652, 4164
Hin1I GRCGYC 4 cut(s) 59, 195, 308, 4387
Hin6I GCGC 8 cut(s) 65, 75, 85, 214, 554, 1539, 3650, 4162
HinP1I GCGC 8 cut(s) 65, 75, 85, 214, 554, 1539, 3650, 4162
HincII GTYRAC 4 cut(s) 178, 1774, 3268, 3994
HindII GTYRAC 4 cut(s) 178, 1774, 3268, 3994
HindIII AAGCTT 2 cut(s) 1889, 2162
HpaI GTTAAC 2 cut(s) 1774, 3268
Hpy166II GTNNAC 8 cut(s) 178, 462, 1774, 1920, 3268, 3434, 3994, 4404
Hpy8I GTNNAC 8 cut(s) 178, 462, 1774, 1920, 3268, 3434, 3994, 4404
HpyCH4IV ACGT 6 cut(s) 308, 464, 2464, 3418, 3766, 4280
HpySE526I ACGT 6 cut(s) 308, 464, 2464, 3418, 3766, 4280
Hsp92I GRCGYC 4 cut(s) 59, 195, 308, 4387
HspAI GCGC 8 cut(s) 65, 75, 85, 214, 554, 1539, 3650, 4162
Kpn2I TCCGGA 1 cut(s) 1216
KpnI GGTACC 2 cut(s) 262, 3888
KspAI GTTAAC 2 cut(s) 1774, 3268
KspI CCGCGG 2 cut(s) 298, 389
LguI GCTCTTC 2 cut(s) 1524, 2457
MaeI CTAG 7 cut(s) 254, 1523, 2355, 2895, 3771, 4044, 4491
MaeII ACGT 6 cut(s) 308, 464, 2464, 3418, 3766, 4280
MbiI CCGCTC 1 cut(s) 117
MfeI CAATTG 2 cut(s) 3240, 4530
MflI RGATCY 7 cut(s) 925, 1360, 1474, 1492, 3123, 3142, 4603
MhlI GDGCHC 9 cut(s) 97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
MlsI TGGCCA 1 cut(s) 4517
MluNI TGGCCA 1 cut(s) 4517
MlyI GAGTC 9 cut(s) 1013, 1268, 1832, 1861, 2302, 2424, 3185, 3917, 4031
MmeI TCCRAC 8 cut(s) 294, 329, 738, 1338, 1524, 2007, 2026, 2643
Mox20I TGGCCA 1 cut(s) 4517
Mph1103I ATGCAT 2 cut(s) 2416, 2980
MroI TCCGGA 1 cut(s) 1216
MroXI GAANNNNTTC 3 cut(s) 3510, 3967, 4563
MscI TGGCCA 1 cut(s) 4517
MslI CAYNNNNRTG 5 cut(s) 1829, 2251, 2445, 3780, 4380
Msp20I TGGCCA 1 cut(s) 4517
MspA1I CMGCKG 4 cut(s) 297, 388, 2314, 2470
MspCI CTTAAG 1 cut(s) 3463
MspR9I CCNGG 7 cut(s) 80, 343, 1472, 1857, 2205, 2907, 3754
MunI CAATTG 2 cut(s) 3240, 4530
Mva1269I GAATGC 4 cut(s) 907, 1666, 2980, 3324
MvaI CCWGG 5 cut(s) 80, 343, 1857, 2205, 2907
MvnI CGCG 8 cut(s) 67, 216, 264, 297, 388, 962, 1541, 2344
NciI CCSGG 2 cut(s) 1472, 3754
NmeAIII GCCGAG 4 cut(s) 71, 79, 571, 1294
NmuCI GTSAC 7 cut(s) 25, 494, 1016, 1065, 2261, 3422, 4534
NotI GCGGCCGC 1 cut(s) 109
NsiI ATGCAT 2 cut(s) 2416, 2980
NspI RCATGY 6 cut(s) 1084, 1195, 2122, 2552, 3641, 4041
OliI CACNNNNGTG 1 cut(s) 1829
PaeI GCATGC 1 cut(s) 2552
PaeR7I CTCGAG 1 cut(s) 3566
PciI ACATGT 1 cut(s) 3637
PciSI GCTCTTC 2 cut(s) 1524, 2457
PcsI WCGNNNNNNNCGW 5 cut(s) 174, 183, 243, 461, 3390
PctI GAATGC 4 cut(s) 907, 1666, 2980, 3324
PdmI GAANNNNTTC 3 cut(s) 3510, 3967, 4563
PflFI GACNNNGTC 2 cut(s) 173, 310
PflMI CCANNNNNTGG 2 cut(s) 3901, 4450
PfoI TCCNGGA 1 cut(s) 1470
PinAI ACCGGT 2 cut(s) 135, 620
Ple19I CGATCG 1 cut(s) 24
PleI GAGTC 9 cut(s) 1013, 1268, 1832, 1860, 2302, 2424, 3185, 3917, 4030
PpsI GAGTC 9 cut(s) 1013, 1268, 1832, 1860, 2302, 2424, 3185, 3917, 4030
PpuMI RGGWCCY 2 cut(s) 1577, 2726
PscI ACATGT 1 cut(s) 3637
PsiI TTATAA 2 cut(s) 3104, 4394
Psp124BI GAGCTC 2 cut(s) 97, 2095
Psp5II RGGWCCY 2 cut(s) 1577, 2726
Psp6I CCWGG 5 cut(s) 78, 341, 1855, 2203, 2905
PspFI CCCAGC 2 cut(s) 1615, 2314
PspGI CCWGG 5 cut(s) 78, 341, 1855, 2203, 2905
PspPPI RGGWCCY 2 cut(s) 1577, 2726
PstI CTGCAG 2 cut(s) 1435, 1936
PsuI RGATCY 7 cut(s) 925, 1360, 1474, 1492, 3123, 3142, 4603
PsyI GACNNNGTC 2 cut(s) 173, 310
PvuI CGATCG 1 cut(s) 24
PvuII CAGCTG 2 cut(s) 2314, 2470
RseI CAYNNNNRTG 5 cut(s) 1829, 2251, 2445, 3780, 4380
SacI GAGCTC 2 cut(s) 97, 2095
SacII CCGCGG 2 cut(s) 298, 389
SalI GTCGAC 1 cut(s) 176
SapI GCTCTTC 2 cut(s) 1524, 2457
SbfI CCTGCAGG 1 cut(s) 1435
ScaI AGTACT 1 cut(s) 3136
SchI GAGTC 9 cut(s) 1013, 1268, 1832, 1861, 2302, 2424, 3185, 3917, 4031
ScrFI CCNGG 7 cut(s) 80, 343, 1472, 1857, 2205, 2907, 3754
SdaI CCTGCAGG 1 cut(s) 1435
SduI GDGCHC 9 cut(s) 97, 592, 825, 1124, 1519, 2095, 2452, 2524, 3734
SfcI CTRYAG 4 cut(s) 1431, 1704, 1932, 4264
Sfr274I CTCGAG 1 cut(s) 3566
Sfr303I CCGCGG 2 cut(s) 298, 389
SgrBI CCGCGG 2 cut(s) 298, 389
SinI GGWCC 8 cut(s) 69, 288, 390, 1109, 1577, 2124, 2726, 4570
SlaI CTCGAG 1 cut(s) 3566
SmiMI CAYNNNNRTG 5 cut(s) 1829, 2251, 2445, 3780, 4380
SmlI CTYRAG 9 cut(s) 1276, 1716, 1966, 2141, 2987, 3070, 3329, 3463, 3566
SmoI CTYRAG 9 cut(s) 1276, 1716, 1966, 2141, 2987, 3070, 3329, 3463, 3566
SphI GCATGC 1 cut(s) 2552
Sse8387I CCTGCAGG 1 cut(s) 1435
SspMI CTAG 7 cut(s) 254, 1523, 2355, 2895, 3771, 4044, 4491
SstI GAGCTC 2 cut(s) 97, 2095
StyD4I CCNGG 7 cut(s) 78, 341, 1470, 1855, 2203, 2905, 3752
StyI CCWWGG 2 cut(s) 253, 2509
TaiI ACGT 6 cut(s) 311, 467, 2467, 3421, 3769, 4283
TaqI TCGA 7 cut(s) 177, 237, 455, 1257, 3393, 3567, 3941
TatI WGTACW 6 cut(s) 1447, 3134, 3309, 4260, 4401, 4419
TscAI CASTG 5 cut(s) 1074, 1129, 1987, 3096, 4382
TseFI GTSAC 7 cut(s) 25, 494, 1016, 1065, 2261, 3422, 4534
Tsp45I GTSAC 7 cut(s) 25, 494, 1016, 1065, 2261, 3422, 4534
TspGWI ACGGA 6 cut(s) 460, 595, 1874, 2635, 3203, 4421
TspRI CASTG 5 cut(s) 1074, 1129, 1987, 3096, 4382
Tth111I GACNNNGTC 2 cut(s) 173, 310
Van91I CCANNNNNTGG 2 cut(s) 3901, 4450
Vha464I CTTAAG 1 cut(s) 3463
VpaK11BI GGWCC 8 cut(s) 69, 288, 390, 1109, 1577, 2124, 2726, 4570
XapI RAATTY 9 cut(s) 1600, 1643, 1942, 2863, 2932, 3471, 3736, 3943, 4521
XceI RCATGY 6 cut(s) 1084, 1195, 2122, 2552, 3641, 4041
XcmI CCANNNNNNNNNTGG 1 cut(s) 2118
XhoI CTCGAG 1 cut(s) 3566
XmaJI CCTAGG 1 cut(s) 253
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 3 cut(s) 3510, 3967, 4563
XspI CTAG 7 cut(s) 254, 1523, 2355, 2895, 3771, 4044, 4491
ZraI GACGTC 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 3136
Zsp2I ATGCAT 2 cut(s) 2416, 2980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.