MD14G1089500.v1.1

Ubiquitin-associated domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015963
Physical Location & Seq
Reverse (-)
11213351 .. 11226116
12766 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1089500.v1.1.491

Sequence Viewer

Length: 879 bp
ATGAACGGCGGTCCATCTGGTTTCCACAATGCTCCTATTACCAGAGCCTTCGTCGTTGCCAGCGGTCTCTTCACCGTCTTCTTTGGGATCCAGGGCCGTTCGAGCAAGCTTGGATTGTCATATCTGGACATATTCGGGAAGTTTCGCCTTTGGAAGTTAATTGTGTCGGTGTTTGCCTTTTCATCTACACCAGAACTGATGTTTGGGCTGTATCTGCTATACTACTTCAGGGTCTTTGAGAGACAGATAGGTTCCAATAAGTACTCAGTGTTCATATTGTACTCTGTGACGGTATCATTACTATTTGAGATTCTTGCTCTAGCAATCCTTAAAGATCCAGCAGTCAACCTATTTACTTCAGGACCTTATGGTCTTATATTTGCATCTTTTGTACCCTTTTTCTTTGATATTCCAGTTTCAACACGCTTTCGTGTATTTGGTGTTCGCTTCTCAGATAAGTCTTTCATATATCTAGCCGGTCTACAGCTTCTTTTATCATCCTGGAAACGATCAATCATACCAGGGATTTTTGGAGTCGTTTGTGGTTCCTTATATCATCTAAATGTCTTCTCTATCCGCAAAGCAAAGTTCCCAGAAGTCATTGCCTCTTTCTTTTCAAGGATTTCTTGGCCATCAATGGGGACTCCACCAGCAGCACCATCTAGAAATATTGTGGGAAATGCACCACCATTCACAGCTCGGCAAGTAGAGAGAAATTATCCCTCTTCCATGCTGTCTGCAACAGAGCCAACAGAGGACTCCATTGGTACTCTGGTTTCTATGGGCTTTGATAGGAACTCTGCCAGACAGGCTCTAGTGCAGGCTAGAAATGACGTCAATGTAGCAACAAACATCCTCCTAGAAGCACATGCCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DER1 Rhomboid UBA

Protein Analysis

293

Amino Acids

32.4

Weight (kDa)

9.95

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 49 - 188 5.2e-06 Rhomboid domain
UBA PF00627 251 - 286 4e-11 UBA/TS-N domain
UBA_7 PF22562 254 - 288 3.1e-06 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015963)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56740
fragaria_vesca FvH4_6g25340
malus_domestica MD12G1096200.v1.1 MD14G1089500.v1.1
prunus_persica Prupe.7G034400_v2.0.a1
pyrus_communis pycom12g08520 pycom14g07930
rosa_chinensis RchiOBHm_Chr2g0125741
rosa_laevigata RLG00000018878
rosa_multiflora Rmu_ssc0000055.1_g000017
rosa_roxburghii Rroxscaffold_2G00117780
rosa_rugosa Rorug02G0258800
rosa_samantha Rh2BG325000 Rh2CG306900
rosa_wichuraiana Rw2G025640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 369
AatII GACGTC 1 cut(s) 837
AccI GTMKAC 1 cut(s) 481
AciI CCGC 3 cut(s) 9, 63, 577
AclWI GGATC 3 cut(s) 82, 95, 329
AcoI YGGCCR 1 cut(s) 629
AcuI CTGAAG 2 cut(s) 211, 342
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 4 cut(s) 263, 281, 393, 769
AfiI CCNNNNNNNGG 1 cut(s) 638
AgsI TTSAA 2 cut(s) 420, 618
AjnI CCWGG 3 cut(s) 90, 500, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AluBI AGCT 3 cut(s) 109, 487, 698
AluI AGCT 3 cut(s) 109, 487, 698
Alw26I GTCTC 2 cut(s) 71, 235
AlwI GGATC 3 cut(s) 82, 95, 329
AoxI GGCC 2 cut(s) 94, 629
ApeKI GCWGC 1 cut(s) 653
AspS9I GGNCC 3 cut(s) 11, 94, 362
AsuHPI GGTGA 1 cut(s) 64
AvaII GGWCC 2 cut(s) 11, 362
BaeI ACNNNNGTAYC 2 cut(s) 759, 792
BalI TGGCCA 1 cut(s) 631
BamHI GGATCC 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 70, 559
BbvI GCAGC 1 cut(s) 665
BccI CCATC 3 cut(s) 22, 640, 667
BceAI ACGGC 2 cut(s) 22, 81
BciT130I CCWGG 3 cut(s) 92, 502, 522
BcoDI GTCTC 2 cut(s) 71, 235
BfaI CTAG 6 cut(s) 320, 473, 663, 815, 825, 860
BfmI CTRYAG 1 cut(s) 482
BglI GCCNNNNNGGC 1 cut(s) 809
BisI GCNGC 1 cut(s) 654
BlsI GCNGC 1 cut(s) 655
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 3 cut(s) 92, 502, 522
Bme18I GGWCC 2 cut(s) 11, 362
BmgT120I GGNCC 3 cut(s) 11, 94, 362
BmiI GGNNCC 3 cut(s) 89, 253, 547
BmrFI CCNGG 3 cut(s) 92, 502, 522
BmsI GCATC 1 cut(s) 392
BpiI GAAGAC 2 cut(s) 70, 559
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 91, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 638
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 3 cut(s) 92, 502, 522
BseDI CCNNGG 2 cut(s) 91, 521
BseGI GGATG 2 cut(s) 497, 852
BseLI CCNNNNNNNGG 1 cut(s) 638
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 2 cut(s) 279, 465
BseNI ACTGG 1 cut(s) 413
BseXI GCAGC 1 cut(s) 665
BsgI GTGCAG 1 cut(s) 839
BshFI GGCC 2 cut(s) 96, 631
BsiSI CCGG 1 cut(s) 477
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 1 cut(s) 638
BsmAI GTCTC 2 cut(s) 71, 235
BsmFI GGGAC 1 cut(s) 655
BsnI GGCC 2 cut(s) 96, 631
Bso31I GGTCTC 1 cut(s) 71
Bsp143I GATC 3 cut(s) 87, 334, 509
BspACI CCGC 3 cut(s) 9, 63, 577
BspANI GGCC 2 cut(s) 96, 631
BspCNI CTCAG 2 cut(s) 278, 464
BspLI GGNNCC 3 cut(s) 89, 253, 547
BspPI GGATC 3 cut(s) 82, 95, 329
BspTNI GGTCTC 1 cut(s) 71
BsrDI GCAATG 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 476
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 91, 521
BssMI GATC 3 cut(s) 87, 334, 509
BssNI GRCGYC 1 cut(s) 834
Bst2UI CCWGG 3 cut(s) 92, 502, 522
Bst4CI ACNGT 2 cut(s) 76, 292
Bst6I CTCTTC 2 cut(s) 74, 730
BstACI GRCGYC 1 cut(s) 834
BstC8I GCNNGC 3 cut(s) 61, 107, 822
BstDEI CTNAG 2 cut(s) 265, 451
BstF5I GGATG 2 cut(s) 497, 852
BstKTI GATC 3 cut(s) 90, 337, 512
BstMAI GTCTC 2 cut(s) 71, 235
BstMBI GATC 3 cut(s) 87, 334, 509
BstMWI GCNNNNNNNGC 3 cut(s) 102, 214, 809
BstNI CCWGG 3 cut(s) 92, 502, 522
BstNSI RCATGY 1 cut(s) 872
BstSCI CCNGG 3 cut(s) 90, 500, 520
BstSFI CTRYAG 1 cut(s) 482
BstV1I GCAGC 1 cut(s) 665
BstV2I GAAGAC 2 cut(s) 70, 559
BstX2I RGATCY 2 cut(s) 87, 334
BstYI RGATCY 2 cut(s) 87, 334
BsuRI GGCC 2 cut(s) 96, 631
BtsCI GGATG 2 cut(s) 497, 852
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 61, 107, 822
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 3 cut(s) 11, 94, 362
Csp6I GTAC 4 cut(s) 262, 280, 392, 768
CviAII CATG 2 cut(s) 730, 869
CviQI GTAC 4 cut(s) 262, 280, 392, 768
DdeI CTNAG 2 cut(s) 265, 451
DpnI GATC 3 cut(s) 89, 336, 511
DpnII GATC 3 cut(s) 87, 334, 509
DrdI GACNNNNNNGTC 1 cut(s) 369
DseDI GACNNNNNNGTC 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 629
Eam1104I CTCTTC 2 cut(s) 74, 730
EarI CTCTTC 2 cut(s) 74, 730
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 2 cut(s) 11, 362
Eco57I CTGAAG 2 cut(s) 211, 342
EcoO109I RGGNCCY 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 90, 500, 520
FaeI CATG 2 cut(s) 733, 872
FalI AAGNNNNNCTT 2 cut(s) 610, 642
FaqI GGGAC 1 cut(s) 655
FatI CATG 2 cut(s) 729, 868
FblI GTMKAC 1 cut(s) 481
Fnu4HI GCNGC 1 cut(s) 654
FokI GGATG 2 cut(s) 484, 839
Fsp4HI GCNGC 1 cut(s) 654
FspBI CTAG 6 cut(s) 320, 473, 663, 815, 825, 860
GluI GCNGC 1 cut(s) 654
HaeIII GGCC 2 cut(s) 96, 631
HapII CCGG 1 cut(s) 477
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 2 cut(s) 733, 872
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 4 cut(s) 310, 534, 643, 758
HpaII CCGG 1 cut(s) 477
HphI GGTGA 1 cut(s) 64
Hpy166II GTNNAC 2 cut(s) 346, 482
Hpy188I TCNGA 1 cut(s) 454
Hpy188III TCNNGA 4 cut(s) 125, 136, 360, 663
Hpy8I GTNNAC 2 cut(s) 346, 482
Hpy99I CGWCG 1 cut(s) 56
HpyAV CCTTC 1 cut(s) 58
HpyCH4III ACNGT 2 cut(s) 76, 292
HpyCH4IV ACGT 1 cut(s) 834
HpyCH4V TGCA 4 cut(s) 383, 683, 740, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 214, 809
HpyF3I CTNAG 2 cut(s) 265, 451
HpySE526I ACGT 1 cut(s) 834
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 2 cut(s) 733, 872
Kzo9I GATC 3 cut(s) 87, 334, 509
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 1 cut(s) 665
LweI GCATC 1 cut(s) 392
MaeI CTAG 6 cut(s) 320, 473, 663, 815, 825, 860
MaeII ACGT 1 cut(s) 834
MaeIII GTNAC 1 cut(s) 286
MalI GATC 3 cut(s) 89, 336, 511
MboI GATC 3 cut(s) 87, 334, 509
MboII GAAGA 4 cut(s) 61, 70, 559, 717
MflI RGATCY 2 cut(s) 87, 334
MlsI TGGCCA 1 cut(s) 631
MluCI AATT 2 cut(s) 159, 715
MluNI TGGCCA 1 cut(s) 631
MlyI GAGTC 3 cut(s) 543, 637, 752
MnlI CCTC 4 cut(s) 616, 733, 748, 866
Mox20I TGGCCA 1 cut(s) 631
MscI TGGCCA 1 cut(s) 631
MseI TTAA 2 cut(s) 158, 330
MslI CAYNNNNRTG 1 cut(s) 561
Msp20I TGGCCA 1 cut(s) 631
MspA1I CMGCKG 1 cut(s) 63
MspI CCGG 1 cut(s) 477
MspR9I CCNGG 3 cut(s) 92, 502, 522
MvaI CCWGG 3 cut(s) 92, 502, 522
MwoI GCNNNNNNNGC 3 cut(s) 102, 214, 809
NdeII GATC 3 cut(s) 87, 334, 509
NlaIII CATG 2 cut(s) 733, 872
NlaIV GGNNCC 3 cut(s) 89, 253, 547
NmeAIII GCCGAG 1 cut(s) 679
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 1 cut(s) 872
PfeI GAWTC 1 cut(s) 310
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 1 cut(s) 655
PleI GAGTC 3 cut(s) 542, 637, 752
PpsI GAGTC 3 cut(s) 542, 637, 752
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 90, 500, 520
PspGI CCWGG 3 cut(s) 90, 500, 520
PspN4I GGNNCC 3 cut(s) 89, 253, 547
PspPI GGNCC 3 cut(s) 11, 94, 362
PspPPI RGGWCCY 1 cut(s) 362
PsrI GAACNNNNNNTAC 4 cut(s) 254, 286, 426, 458
PsuI RGATCY 2 cut(s) 87, 334
RsaI GTAC 4 cut(s) 263, 281, 393, 769
RsaNI GTAC 4 cut(s) 262, 280, 392, 768
RseI CAYNNNNRTG 1 cut(s) 561
SaqAI TTAA 2 cut(s) 158, 330
SatI GCNGC 1 cut(s) 654
Sau3AI GATC 3 cut(s) 87, 334, 509
Sau96I GGNCC 3 cut(s) 11, 94, 362
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 3 cut(s) 543, 637, 752
ScrFI CCNGG 3 cut(s) 92, 502, 522
SetI ASST 7 cut(s) 111, 253, 351, 367, 489, 700, 837
SfaNI GCATC 1 cut(s) 392
SfcI CTRYAG 1 cut(s) 482
SinI GGWCC 2 cut(s) 11, 362
SmiMI CAYNNNNRTG 1 cut(s) 561
Sse9I AATT 2 cut(s) 159, 715
SsiI CCGC 3 cut(s) 9, 63, 577
SspI AATATT 1 cut(s) 670
SspMI CTAG 6 cut(s) 320, 473, 663, 815, 825, 860
StyD4I CCNGG 3 cut(s) 90, 500, 520
TaaI ACNGT 2 cut(s) 76, 292
TaiI ACGT 1 cut(s) 837
TaqI TCGA 1 cut(s) 101
TasI AATT 2 cut(s) 159, 715
TatI WGTACW 2 cut(s) 261, 279
TfiI GAWTC 1 cut(s) 310
Tru1I TTAA 2 cut(s) 158, 330
Tru9I TTAA 2 cut(s) 158, 330
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 1 cut(s) 653
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 4 cut(s) 17, 171, 262, 454
TspRI CASTG 1 cut(s) 273
VpaK11BI GGWCC 2 cut(s) 11, 362
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 1 cut(s) 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 481
XspI CTAG 6 cut(s) 320, 473, 663, 815, 825, 860
ZraI GACGTC 1 cut(s) 835
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.