Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0015511

Physical Location & Seq

Genomic Coordinates Reverse (-)

17275410 .. 17277516

Gene Structure 2107 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1109000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 399 bp

ATGGCTAAGAGCATCTCCTACATTACGGGCTCTCAGCTTCTCTCCCTCAAACCCCGCCCCAACATCGCCATCATCGACGTCAGGGACGACGAGCGGAGCTGCGACGGGCACATAGCAGGATCTTTGCACTACCCGAGTGGGAATTTCTACGACAAGATCTCCAATCTTGCTCAAGATGTCAAAGGCAAAGACACCCTCGTTTTCCACTGCGCTCTTAGCCAGGTTCGGGGTCCATCCTGTGCACGAAAGTTTGCCAATTACCTTGCAGAGGTGAAGGAAGAGACTGGAATCAAAGACATTTTGATTTTGGAGCGTGGCTTCAATGGCTGGCAAAGTTCCGGTAGACCTGTTTGCCACTGCAACAGCATTCCCTGTAAAGGTGAGAGCGGGAGTGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004725 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008794 GO:0009507 GO:0009536 GO:0009987 GO:0016310 GO:0016311 GO:0016491 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0030611 GO:0030613 GO:0030614 GO:0035335 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046685 GO:0050896 GO:0055114 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhodanese

Protein Analysis

133

Amino Acids

14.37

Weight (kDa)

8.23

Isoelectric Point (pI)

47.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhodanese	PF00581	12 - 112	2e-12	Rhodanese-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0015511)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G03455 AT5G03455
fragaria_vesca	FvH4_6g16580
malus_domestica	MD12G1109000.v1.1
prunus_persica	Prupe.7G127500_v2.0.a1
pyrus_communis	pycom12g10920
rosa_chinensis	RchiOBHm_Chr3g0470511
rosa_laevigata	RLG00000024261
rosa_multiflora	Rmu_sc0003381.1_g000014
rosa_roxburghii	Rroxscaffold_6G00410750
rosa_rugosa	Rorug03G0109800
rosa_samantha	Rh3AG159900 Rh3BG184400 Rh3CG174900 Rh3DG190300
rosa_wichuraiana	Rw3G015130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	81
AccBSI	CCGCTC	2 cut(s)	94, 387
AccI	GTMKAC	1 cut(s)	343
AciI	CCGC	3 cut(s)	55, 94, 387
AclWI	GGATC	1 cut(s)	127
AcsI	RAATTY	1 cut(s)	142
AcyI	GRCGYC	1 cut(s)	78
AfiI	CCNNNNNNNGG	3 cut(s)	226, 268, 377
AgsI	TTSAA	1 cut(s)	322
AjnI	CCWGG	1 cut(s)	219
AluBI	AGCT	2 cut(s)	37, 99
AluI	AGCT	2 cut(s)	37, 99
Alw21I	GWGCWC	1 cut(s)	244
Alw26I	GTCTC	1 cut(s)	275
Alw44I	GTGCAC	1 cut(s)	240
AlwI	GGATC	1 cut(s)	127
Ama87I	CYCGRG	1 cut(s)	133
ApaLI	GTGCAC	1 cut(s)	240
ApeKI	GCWGC	1 cut(s)	99
ApoI	RAATTY	1 cut(s)	142
AspLEI	GCGC	1 cut(s)	212
AspS9I	GGNCC	1 cut(s)	230
AsuHPI	GGTGA	2 cut(s)	283, 392
AvaI	CYCGRG	1 cut(s)	133
AvaII	GGWCC	1 cut(s)	230
BaeGI	GKGCMC	2 cut(s)	111, 244
BanII	GRGCYC	1 cut(s)	32
Bbv12I	GWGCWC	1 cut(s)	244
BbvI	GCAGC	1 cut(s)	86
BccI	CCATC	2 cut(s)	77, 241
BciT130I	CCWGG	1 cut(s)	221
BcoDI	GTCTC	1 cut(s)	275
BglII	AGATCT	1 cut(s)	156
BisI	GCNGC	1 cut(s)	100
BlsI	GCNGC	1 cut(s)	101
Bme1390I	CCNGG	1 cut(s)	221
Bme18I	GGWCC	1 cut(s)	230
BmeT110I	CYCGRG	1 cut(s)	133
BmgT120I	GGNCC	1 cut(s)	230
BmiI	GGNNCC	1 cut(s)	231
BmrFI	CCNGG	1 cut(s)	221
BmsI	GCATC	1 cut(s)	21
BpuEI	CTTGAG	1 cut(s)	156
BsaHI	GRCGYC	1 cut(s)	78
BsaWI	WCCGGW	1 cut(s)	338
BsaXI	ACNNNNNCTCC	2 cut(s)	143, 173
Bsc4I	CCNNNNNNNGG	3 cut(s)	226, 268, 377
Bse1I	ACTGG	1 cut(s)	289
BseBI	CCWGG	1 cut(s)	221
BseGI	GGATG	1 cut(s)	233
BseLI	CCNNNNNNNGG	3 cut(s)	226, 268, 377
BseMII	CTCAG	1 cut(s)	47
BseNI	ACTGG	1 cut(s)	289
BseSI	GKGCMC	2 cut(s)	111, 244
BseXI	GCAGC	1 cut(s)	86
BsiHKAI	GWGCWC	1 cut(s)	244
BsiHKCI	CYCGRG	1 cut(s)	133
BsiSI	CCGG	1 cut(s)	339
BslFI	GGGAC	1 cut(s)	98
BslI	CCNNNNNNNGG	3 cut(s)	226, 268, 377
BsmAI	GTCTC	1 cut(s)	275
BsmFI	GGGAC	1 cut(s)	98
BsmI	GAATGC	1 cut(s)	366
BsoBI	CYCGRG	1 cut(s)	133
Bsp1286I	GDGCHC	3 cut(s)	32, 111, 244
Bsp143I	GATC	2 cut(s)	119, 156
BspACI	CCGC	3 cut(s)	55, 94, 387
BspCNI	CTCAG	1 cut(s)	46
BspLI	GGNNCC	1 cut(s)	231
BspPI	GGATC	1 cut(s)	127
BsrBI	CCGCTC	2 cut(s)	94, 387
BsrI	ACTGG	1 cut(s)	289
BssMI	GATC	2 cut(s)	119, 156
BssNI	GRCGYC	1 cut(s)	78
Bst2UI	CCWGG	1 cut(s)	221
Bst6I	CTCTTC	1 cut(s)	273
BstACI	GRCGYC	1 cut(s)	78
BstC8I	GCNNGC	1 cut(s)	329
BstDEI	CTNAG	3 cut(s)	6, 33, 215
BstENI	CCTNNNNNAGG	1 cut(s)	266
BstF5I	GGATG	1 cut(s)	233
BstHHI	GCGC	1 cut(s)	212
BstKTI	GATC	2 cut(s)	122, 159
BstMAI	GTCTC	1 cut(s)	275
BstMBI	GATC	2 cut(s)	119, 156
BstMWI	GCNNNNNNNGC	2 cut(s)	216, 324
BstNI	CCWGG	1 cut(s)	221
BstSCI	CCNGG	1 cut(s)	219
BstSLI	GKGCMC	2 cut(s)	111, 244
BstV1I	GCAGC	1 cut(s)	86
BstX2I	RGATCY	2 cut(s)	119, 156
BstYI	RGATCY	2 cut(s)	119, 156
BtgZI	GCGATG	1 cut(s)	49
BtsCI	GGATG	1 cut(s)	233
BtsI	GCAGTG	2 cut(s)	205, 355
BtsIMutI	CAGTG	2 cut(s)	205, 355
Cac8I	GCNNGC	1 cut(s)	329
CfoI	GCGC	1 cut(s)	212
Cfr13I	GGNCC	1 cut(s)	230
CviJI	RGCY	7 cut(s)	5, 30, 37, 99, 219, 318, 327
CviKI_1	RGCY	7 cut(s)	5, 30, 37, 99, 219, 318, 327
DdeI	CTNAG	3 cut(s)	6, 33, 215
DpnI	GATC	2 cut(s)	121, 158
DpnII	GATC	2 cut(s)	119, 156
Eam1104I	CTCTTC	1 cut(s)	273
EarI	CTCTTC	1 cut(s)	273
Eco24I	GRGCYC	1 cut(s)	32
Eco47I	GGWCC	1 cut(s)	230
Eco88I	CYCGRG	1 cut(s)	133
EcoNI	CCTNNNNNAGG	1 cut(s)	266
EcoRII	CCWGG	1 cut(s)	219
EcoT38I	GRGCYC	1 cut(s)	32
FaiI	YATR	1 cut(s)	113
FaqI	GGGAC	1 cut(s)	98
FauI	CCCGC	2 cut(s)	62, 380
FblI	GTMKAC	1 cut(s)	343
Fnu4HI	GCNGC	1 cut(s)	100
FokI	GGATG	1 cut(s)	220
FriOI	GRGCYC	1 cut(s)	32
Fsp4HI	GCNGC	1 cut(s)	100
GlaI	GCGC	1 cut(s)	211
GluI	GCNGC	1 cut(s)	100
HapII	CCGG	1 cut(s)	339
HhaI	GCGC	1 cut(s)	212
Hin1I	GRCGYC	1 cut(s)	78
Hin6I	GCGC	1 cut(s)	210
HinP1I	GCGC	1 cut(s)	210
HinfI	GANTC	1 cut(s)	288
HpaII	CCGG	1 cut(s)	339
HphI	GGTGA	2 cut(s)	283, 392
Hpy166II	GTNNAC	2 cut(s)	242, 344
Hpy188III	TCNNGA	1 cut(s)	173
Hpy8I	GTNNAC	2 cut(s)	242, 344
Hpy99I	CGWCG	3 cut(s)	80, 92, 107
HpyAV	CCTTC	1 cut(s)	268
HpyCH4IV	ACGT	1 cut(s)	78
HpyCH4V	TGCA	4 cut(s)	127, 242, 266, 360
HpyF10VI	GCNNNNNNNGC	2 cut(s)	216, 324
HpyF3I	CTNAG	3 cut(s)	6, 33, 215
HpySE526I	ACGT	1 cut(s)	78
Hsp92I	GRCGYC	1 cut(s)	78
HspAI	GCGC	1 cut(s)	210
Kzo9I	GATC	2 cut(s)	119, 156
LmnI	GCTCC	2 cut(s)	96, 310
Lsp1109I	GCAGC	1 cut(s)	86
LweI	GCATC	1 cut(s)	21
MaeII	ACGT	1 cut(s)	78
MalI	GATC	2 cut(s)	121, 158
MbiI	CCGCTC	2 cut(s)	94, 387
MboI	GATC	2 cut(s)	119, 156
MboII	GAAGA	1 cut(s)	290
MflI	RGATCY	2 cut(s)	119, 156
MhlI	GDGCHC	3 cut(s)	32, 111, 244
MluCI	AATT	2 cut(s)	142, 256
MnlI	CCTC	3 cut(s)	56, 206, 262
MspI	CCGG	1 cut(s)	339
MspR9I	CCNGG	1 cut(s)	221
Mva1269I	GAATGC	1 cut(s)	366
MvaI	CCWGG	1 cut(s)	221
MwoI	GCNNNNNNNGC	2 cut(s)	216, 324
NdeII	GATC	2 cut(s)	119, 156
NlaIV	GGNNCC	1 cut(s)	231
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 84
PctI	GAATGC	1 cut(s)	366
PfeI	GAWTC	1 cut(s)	288
PkrI	GCNGC	1 cut(s)	101
Psp6I	CCWGG	1 cut(s)	219
PspGI	CCWGG	1 cut(s)	219
PspN4I	GGNNCC	1 cut(s)	231
PspPI	GGNCC	1 cut(s)	230
PsuI	RGATCY	2 cut(s)	119, 156
SatI	GCNGC	1 cut(s)	100
Sau3AI	GATC	2 cut(s)	119, 156
Sau96I	GGNCC	1 cut(s)	230
ScrFI	CCNGG	1 cut(s)	221
SduI	GDGCHC	3 cut(s)	32, 111, 244
SetI	ASST	8 cut(s)	39, 81, 101, 225, 264, 273, 349, 382
SfaNI	GCATC	1 cut(s)	21
SinI	GGWCC	1 cut(s)	230
SmlI	CTYRAG	1 cut(s)	171
SmoI	CTYRAG	1 cut(s)	171
Sse9I	AATT	2 cut(s)	142, 256
SsiI	CCGC	3 cut(s)	55, 94, 387
StyD4I	CCNGG	1 cut(s)	219
TaiI	ACGT	1 cut(s)	81
TaqI	TCGA	1 cut(s)	75
TasI	AATT	2 cut(s)	142, 256
TfiI	GAWTC	1 cut(s)	288
TscAI	CASTG	2 cut(s)	212, 362
TseI	GCWGC	1 cut(s)	99
TspRI	CASTG	2 cut(s)	212, 362
VneI	GTGCAC	1 cut(s)	240
VpaK11BI	GGWCC	1 cut(s)	230
XagI	CCTNNNNNAGG	1 cut(s)	266
XapI	RAATTY	1 cut(s)	142
XmiI	GTMKAC	1 cut(s)	343
ZraI	GACGTC	1 cut(s)	79

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.