Rh3DG190300

Dual specificity phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0015511
Physical Location & Seq
Reverse (-)
16958878 .. 16960608
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG190300.1

Sequence Viewer

Length: 525 bp
ATGTGGGAGGTCCGTAGCCCAAAGGGAGCATCCCATTCCCACTCCTGGTTGTGCTTTCACAGAAACGAGAAGAAGAAGAAAACGAAGGAATCATCACCAGATATGGCTCGGAGCATCTCCTACATAACAGGGTCTCAGCTCCTCTCTCTCAGGCGCCGCCCCAACAATATCGCCATCGTCGATGTCAGGGATGATGAAAGGAGCTACGATGGGCACATAGCAGGGTCTTTGCACTACCCCAATGACAGTTTCTATGACAAGATCTCCAATCTCGCTCAAGAAGTCAAAGGCAAAGACACCCTCGTTTTCCACTGCGCTCTCAGCCAGGTCCGAGGCCCGTCCTGTGCGCGGAAGTTTGCCAATTATCTTGAGGAGATGAAGGAAGATACAGGAATCAAAGACATTTTGGTTTTGGAGCGTGGCTTCAATGGCTGGGAATCTTCTGGTAGACCTGTTTGTCGCTGCAACAGCATTCCCTGTAAGGGTGAGATTCAGACTGAGACTGAGACTGAGAAGAAAGCATAA

Protein Analysis

174

Amino Acids

19.78

Weight (kDa)

8.71

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 49 - 147 1e-11 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015511)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G03455 AT5G03455
fragaria_vesca FvH4_6g16580
malus_domestica MD12G1109000.v1.1
prunus_persica Prupe.7G127500_v2.0.a1
pyrus_communis pycom12g10920
rosa_chinensis RchiOBHm_Chr3g0470511
rosa_laevigata RLG00000024261
rosa_multiflora Rmu_sc0003381.1_g000014
rosa_roxburghii Rroxscaffold_6G00410750
rosa_rugosa Rorug03G0109800
rosa_samantha Rh3AG159900 Rh3BG184400 Rh3CG174900 Rh3DG190300
rosa_wichuraiana Rw3G015130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 456
AccB1I GGYRCC 1 cut(s) 153
AccI GTMKAC 1 cut(s) 448
AccII CGCG 1 cut(s) 349
AciI CCGC 2 cut(s) 157, 349
AcyI GRCGYC 1 cut(s) 154
AfiI CCNNNNNNNGG 3 cut(s) 45, 348, 482
AgsI TTSAA 1 cut(s) 427
AjnI CCWGG 2 cut(s) 44, 324
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 2 cut(s) 139, 204
AluI AGCT 2 cut(s) 139, 204
Alw26I GTCTC 3 cut(s) 138, 494, 500
AoxI GGCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 462
AspLEI GCGC 3 cut(s) 156, 317, 349
AspS9I GGNCC 3 cut(s) 10, 328, 335
AsuHPI GGTGA 2 cut(s) 87, 497
AvaII GGWCC 2 cut(s) 10, 328
BaeGI GKGCMC 1 cut(s) 216
BanI GGYRCC 1 cut(s) 153
BbvI GCAGC 1 cut(s) 449
BccI CCATC 2 cut(s) 182, 203
BciT130I CCWGG 2 cut(s) 46, 326
BcoDI GTCTC 3 cut(s) 138, 494, 500
BfoI RGCGCY 1 cut(s) 157
BglII AGATCT 1 cut(s) 261
BisI GCNGC 2 cut(s) 157, 463
BlsI GCNGC 2 cut(s) 158, 464
Bme1390I CCNGG 2 cut(s) 46, 326
Bme18I GGWCC 2 cut(s) 10, 328
BmgT120I GGNCC 3 cut(s) 10, 328, 335
BmiI GGNNCC 1 cut(s) 155
BmrFI CCNGG 2 cut(s) 46, 326
BmsI GCATC 2 cut(s) 38, 123
BpuEI CTTGAG 2 cut(s) 261, 389
BsaHI GRCGYC 1 cut(s) 154
BsaI GGTCTC 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 331
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 348, 482
BseBI CCWGG 2 cut(s) 46, 326
BseDI CCNNGG 1 cut(s) 331
BseGI GGATG 2 cut(s) 29, 196
BseLI CCNNNNNNNGG 3 cut(s) 45, 348, 482
BseMII CTCAG 6 cut(s) 149, 163, 334, 489, 495, 501
BseRI GAGGAG 2 cut(s) 131, 386
BseSI GKGCMC 1 cut(s) 216
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 1 cut(s) 432
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 1 cut(s) 336
BshNI GGYRCC 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 45, 348, 482
BsmAI GTCTC 3 cut(s) 138, 494, 500
BsmI GAATGC 1 cut(s) 471
BsnI GGCC 1 cut(s) 336
Bso31I GGTCTC 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 2 cut(s) 157, 349
BspANI GGCC 1 cut(s) 336
BspCNI CTCAG 6 cut(s) 148, 162, 333, 490, 496, 502
BspFNI CGCG 1 cut(s) 349
BspLI GGNNCC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 153
BspTNI GGTCTC 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 331
BssMI GATC 1 cut(s) 261
BssNI GRCGYC 1 cut(s) 154
Bst2UI CCWGG 2 cut(s) 46, 326
Bst4CI ACNGT 1 cut(s) 248
BstACI GRCGYC 1 cut(s) 154
BstDEI CTNAG 6 cut(s) 135, 149, 320, 498, 504, 510
BstF5I GGATG 2 cut(s) 29, 196
BstFNI CGCG 1 cut(s) 349
BstH2I RGCGCY 1 cut(s) 157
BstHHI GCGC 3 cut(s) 156, 317, 349
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 3 cut(s) 138, 494, 500
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 321, 429, 468
BstNI CCWGG 2 cut(s) 46, 326
BstSCI CCNGG 2 cut(s) 44, 324
BstSLI GKGCMC 1 cut(s) 216
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 449
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 1 cut(s) 336
BtsCI GGATG 2 cut(s) 29, 196
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
CfoI GCGC 3 cut(s) 156, 317, 349
Cfr13I GGNCC 3 cut(s) 10, 328, 335
CviJI RGCY 8 cut(s) 18, 107, 139, 204, 324, 336, 423, 432
CviKI_1 RGCY 8 cut(s) 18, 107, 139, 204, 324, 336, 423, 432
DdeI CTNAG 6 cut(s) 135, 149, 320, 498, 504, 510
DinI GGCGCC 1 cut(s) 155
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DrdI GACNNNNNNGTC 1 cut(s) 456
DseDI GACNNNNNNGTC 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 138
Eco47I GGWCC 2 cut(s) 10, 328
EcoRII CCWGG 2 cut(s) 44, 324
EgeI GGCGCC 1 cut(s) 155
EheI GGCGCC 1 cut(s) 155
FaiI YATR 5 cut(s) 104, 125, 218, 255, 523
FblI GTMKAC 1 cut(s) 448
Fnu4HI GCNGC 2 cut(s) 157, 463
FokI GGATG 2 cut(s) 16, 203
Fsp4HI GCNGC 2 cut(s) 157, 463
GlaI GCGC 3 cut(s) 155, 316, 348
GluI GCNGC 2 cut(s) 157, 463
GsaI CCCAGC 1 cut(s) 436
HaeII RGCGCY 1 cut(s) 157
HaeIII GGCC 1 cut(s) 336
HhaI GCGC 3 cut(s) 156, 317, 349
Hin1I GRCGYC 1 cut(s) 154
Hin6I GCGC 3 cut(s) 154, 315, 347
HinP1I GCGC 3 cut(s) 154, 315, 347
HinfI GANTC 4 cut(s) 89, 393, 437, 490
HphI GGTGA 2 cut(s) 87, 497
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 3 cut(s) 111, 332, 495
Hpy188III TCNNGA 2 cut(s) 278, 368
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 2 cut(s) 79, 373
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4V TGCA 2 cut(s) 232, 465
HpyF10VI GCNNNNNNNGC 3 cut(s) 321, 429, 468
HpyF3I CTNAG 6 cut(s) 135, 149, 320, 498, 504, 510
Hsp92I GRCGYC 1 cut(s) 154
HspAI GCGC 3 cut(s) 154, 315, 347
KasI GGCGCC 1 cut(s) 153
Kzo9I GATC 1 cut(s) 261
LmnI GCTCC 5 cut(s) 26, 111, 144, 201, 415
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 2 cut(s) 38, 123
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 5 cut(s) 82, 85, 88, 395, 432
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 1 cut(s) 361
Mly113I GGCGCC 1 cut(s) 154
MnlI CCTC 4 cut(s) 152, 311, 326, 364
MspR9I CCNGG 2 cut(s) 46, 326
Mva1269I GAATGC 1 cut(s) 471
MvaI CCWGG 2 cut(s) 46, 326
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 3 cut(s) 321, 429, 468
NarI GGCGCC 1 cut(s) 154
NdeII GATC 1 cut(s) 261
NlaIV GGNNCC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 1 cut(s) 471
PfeI GAWTC 4 cut(s) 89, 393, 437, 490
PkrI GCNGC 2 cut(s) 158, 464
PluTI GGCGCC 1 cut(s) 157
Psp6I CCWGG 2 cut(s) 44, 324
PspFI CCCAGC 1 cut(s) 432
PspGI CCWGG 2 cut(s) 44, 324
PspN4I GGNNCC 1 cut(s) 155
PspPI GGNCC 3 cut(s) 10, 328, 335
PsuI RGATCY 1 cut(s) 261
SatI GCNGC 2 cut(s) 157, 463
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 3 cut(s) 10, 328, 335
ScrFI CCNGG 2 cut(s) 46, 326
SduI GDGCHC 1 cut(s) 216
SetI ASST 5 cut(s) 12, 141, 206, 330, 454
SfaNI GCATC 2 cut(s) 38, 123
SfoI GGCGCC 1 cut(s) 155
SinI GGWCC 2 cut(s) 10, 328
SmlI CTYRAG 2 cut(s) 276, 368
SmoI CTYRAG 2 cut(s) 276, 368
Sse9I AATT 1 cut(s) 361
SsiI CCGC 2 cut(s) 157, 349
SspDI GGCGCC 1 cut(s) 153
StyD4I CCNGG 2 cut(s) 44, 324
TaaI ACNGT 1 cut(s) 248
TaqI TCGA 1 cut(s) 180
TasI AATT 1 cut(s) 361
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 4 cut(s) 89, 393, 437, 490
TscAI CASTG 1 cut(s) 317
TseI GCWGC 1 cut(s) 462
TspDTI ATGAA 2 cut(s) 210, 392
TspRI CASTG 1 cut(s) 317
VpaK11BI GGWCC 2 cut(s) 10, 328
XmiI GTMKAC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.