MD12G1110000.v1.1

Protein of unknown function (DUF4079)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0017132
Physical Location & Seq
Reverse (-)
17405593 .. 17408025
2433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1110000.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGCTATGGTTACTGCCTCTTCTCACCTCTCTCTGAATCTGTCTCCTCCTCTTGTGCTTCGTGCCTCTCGATTTCCGACCAATGCTCGACCACCTTCCTCCAAATGCTTCCCTGCGCCAATGCCACCAGTAACTTCCGCGTCAGCAAGTAGCAGAAGTAGAAAAACTCTCCTATCTCCGTTTGTGATCAACAACGACAAATTCAGTTTAGACATTGTTGAAGATGATGACGCGGCTGACCAAAAACAACAAGTGGGGAATGCCAATGCGAATGAGACCCTTCTCTACTCTTCAGTCCTTTGCCTCTGCTGCTCCTGGCTGTTCTTCCTGGAGGAATTTTCTGCTACTGTGGCATCTCTGTTCGAGCCTTTCGTTGAGCTTGTCAAATCGTTTGGTCTTCCTGGCTGGCTAGTACATTGGGGTCACCCGGGCAACATGGCTGTTGTGCTTTTCGCTATGGGCGGCTATGGAACATATCTTGGTTATCGGATACGCTTTTCAGATGATGTGGAGGAGAGGGCCCATGCCAAAGACTTGCATCCCAAGCTTCTTGCAGGGATGTTTTTCTTCTTTGCTCTTGGAGCAACTGGAGGAGTAACGTCTCTTCTCACTTCGGACAAATCCATTTTTGAAAGCCCTCATGCTGTTACAGGGATCATTGGTCTTAGCCTCTTGACCATACAAACAATTTTACCTGCTTTGTTTGAGGGTAAACCTGAATTAAGAACAGTTCATGGGGTGTTGGGCACTGGGATCATGACATTGTTTGTTGTCCATGCTGCCCTTGGACTTCAACTTGGCCTAAGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4079

Protein Analysis

271

Amino Acids

29.13

Weight (kDa)

6.22

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4079 PF13301 138 - 270 2.1e-26 Protein of unknown function (DUF4079)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017132)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36885 AT2G36885
malus_domestica MD12G1110000.v1.1
prunus_persica Prupe.7G128600_v2.0.a1
pyrus_communis pycom12g11090
rosa_chinensis RchiOBHm_Chr3g0470401
rosa_laevigata RLG00000024270
rosa_multiflora Rmu_sc0002383.1_g000015
rosa_roxburghii Rroxscaffold_6G00410850
rosa_rugosa Rorug03G0108400
rosa_samantha Rh3AG158800 Rh3DG191500
rosa_wichuraiana Rw3G015060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 703
AccII CGCG 2 cut(s) 140, 233
AciI CCGC 3 cut(s) 138, 233, 462
AclWI GGATC 2 cut(s) 662, 761
AcsI RAATTY 2 cut(s) 200, 335
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 1 cut(s) 414
AgsI TTSAA 3 cut(s) 221, 632, 794
AjnI CCWGG 3 cut(s) 314, 327, 400
AluBI AGCT 3 cut(s) 379, 547, 807
AluI AGCT 3 cut(s) 379, 547, 807
Alw26I GTCTC 3 cut(s) 48, 269, 606
AlwI GGATC 2 cut(s) 662, 761
Ama87I CYCGRG 1 cut(s) 427
AoxI GGCC 2 cut(s) 519, 799
ApaI GGGCCC 1 cut(s) 523
ApeKI GCWGC 2 cut(s) 309, 779
ApoI RAATTY 2 cut(s) 200, 335
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 519, 520
AsuC2I CCSGG 2 cut(s) 428, 429
AsuHPI GGTGA 2 cut(s) 17, 416
AvaI CYCGRG 1 cut(s) 427
BaeGI GKGCMC 2 cut(s) 523, 749
BanII GRGCYC 1 cut(s) 523
BarI GAAGNNNNNNTAC 2 cut(s) 588, 620
BbsI GAAGAC 1 cut(s) 389
BbvI GCAGC 2 cut(s) 296, 766
BciT130I CCWGG 3 cut(s) 316, 329, 402
BciVI GTATCC 1 cut(s) 483
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 2 cut(s) 428, 429
BcoDI GTCTC 3 cut(s) 48, 269, 606
BfaI CTAG 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 703
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 4 cut(s) 234, 310, 463, 780
BlsI GCNGC 4 cut(s) 235, 311, 464, 781
Bme1390I CCNGG 5 cut(s) 316, 329, 402, 428, 429
BmeT110I CYCGRG 1 cut(s) 427
BmgT120I GGNCC 2 cut(s) 519, 520
BmiI GGNNCC 1 cut(s) 521
BmrFI CCNGG 5 cut(s) 316, 329, 402, 428, 429
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 2 cut(s) 362, 547
BmuI ACTGGG 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 389
BpmI CTGGAG 2 cut(s) 350, 609
Bpu10I CCTNAGC 1 cut(s) 803
BpuMI CCSGG 2 cut(s) 428, 429
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 427, 784
Bse1I ACTGG 3 cut(s) 128, 592, 754
BseBI CCWGG 3 cut(s) 316, 329, 402
BseDI CCNNGG 2 cut(s) 427, 784
BseGI GGATG 2 cut(s) 538, 564
BseNI ACTGG 3 cut(s) 128, 592, 754
BseRI GAGGAG 4 cut(s) 36, 39, 527, 606
BseSI GKGCMC 2 cut(s) 523, 749
BseXI GCAGC 2 cut(s) 296, 766
Bsh1236I CGCG 2 cut(s) 140, 233
BshFI GGCC 2 cut(s) 521, 801
BsiHKCI CYCGRG 1 cut(s) 427
BsiSI CCGG 1 cut(s) 428
BsmAI GTCTC 3 cut(s) 48, 269, 606
BsmBI CGTCTC 1 cut(s) 606
BsmI GAATGC 1 cut(s) 265
BsnI GGCC 2 cut(s) 521, 801
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 427
Bsp120I GGGCCC 1 cut(s) 519
Bsp1286I GDGCHC 2 cut(s) 523, 749
Bsp143I GATC 3 cut(s) 186, 654, 753
BspACI CCGC 3 cut(s) 138, 233, 462
BspANI GGCC 2 cut(s) 521, 801
BspFNI CGCG 2 cut(s) 140, 233
BspHI TCATGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 521
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 2 cut(s) 662, 761
BspTNI GGTCTC 1 cut(s) 269
BsrI ACTGG 3 cut(s) 128, 592, 754
BssECI CCNNGG 2 cut(s) 427, 784
BssMI GATC 3 cut(s) 186, 654, 753
BssT1I CCWWGG 1 cut(s) 784
Bst2UI CCWGG 3 cut(s) 316, 329, 402
Bst4CI ACNGT 2 cut(s) 349, 730
Bst6I CTCTTC 3 cut(s) 25, 295, 609
BstC8I GCNNGC 1 cut(s) 407
BstDEI CTNAG 2 cut(s) 665, 803
BstEII GGTNACC 1 cut(s) 422
BstF5I GGATG 2 cut(s) 538, 564
BstFNI CGCG 2 cut(s) 140, 233
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 3 cut(s) 189, 657, 756
BstMAI GTCTC 3 cut(s) 48, 269, 606
BstMBI GATC 3 cut(s) 186, 654, 753
BstMWI GCNNNNNNNGC 4 cut(s) 309, 350, 544, 581
BstNI CCWGG 3 cut(s) 316, 329, 402
BstPI GGTNACC 1 cut(s) 422
BstSCI CCNGG 5 cut(s) 314, 327, 400, 426, 427
BstSLI GKGCMC 2 cut(s) 523, 749
BstUI CGCG 2 cut(s) 140, 233
BstV1I GCAGC 2 cut(s) 296, 766
BstV2I GAAGAC 1 cut(s) 389
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 2 cut(s) 521, 801
BtsCI GGATG 2 cut(s) 538, 564
BtsIMutI CAGTG 1 cut(s) 747
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 1 cut(s) 407
CciI TCATGA 1 cut(s) 756
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 519, 520
Cfr9I CCCGGG 1 cut(s) 427
CseI GACGC 2 cut(s) 129, 239
Csp6I GTAC 1 cut(s) 413
CviAII CATG 6 cut(s) 436, 524, 641, 734, 757, 776
CviQI GTAC 1 cut(s) 413
DdeI CTNAG 2 cut(s) 665, 803
DpnI GATC 3 cut(s) 188, 656, 755
DpnII GATC 3 cut(s) 186, 654, 753
Eam1104I CTCTTC 3 cut(s) 25, 295, 609
EarI CTCTTC 3 cut(s) 25, 295, 609
Eco130I CCWWGG 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 427
Eco91I GGTNACC 1 cut(s) 422
EcoO109I RGGNCCY 1 cut(s) 519
EcoO65I GGTNACC 1 cut(s) 422
EcoRII CCWGG 3 cut(s) 314, 327, 400
EcoT14I CCWWGG 1 cut(s) 784
EcoT38I GRGCYC 1 cut(s) 523
ErhI CCWWGG 1 cut(s) 784
Esp3I CGTCTC 1 cut(s) 606
FaeI CATG 6 cut(s) 439, 527, 644, 737, 760, 779
FatI CATG 6 cut(s) 435, 523, 640, 733, 756, 775
FbaI TGATCA 1 cut(s) 186
Fnu4HI GCNGC 4 cut(s) 234, 310, 463, 780
FokI GGATG 2 cut(s) 525, 571
FriOI GRGCYC 1 cut(s) 523
Fsp4HI GCNGC 4 cut(s) 234, 310, 463, 780
FspBI CTAG 1 cut(s) 410
GlaI GCGC 1 cut(s) 117
GluI GCNGC 4 cut(s) 234, 310, 463, 780
GsuI CTGGAG 2 cut(s) 350, 609
HaeIII GGCC 2 cut(s) 521, 801
HapII CCGG 1 cut(s) 428
HgaI GACGC 2 cut(s) 129, 239
HhaI GCGC 1 cut(s) 118
Hin1II CATG 6 cut(s) 439, 527, 644, 737, 760, 779
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 1 cut(s) 37
HpaII CCGG 1 cut(s) 428
HphI GGTGA 2 cut(s) 17, 416
Hpy166II GTNNAC 1 cut(s) 713
Hpy188I TCNGA 5 cut(s) 36, 78, 489, 502, 616
Hpy188III TCNNGA 3 cut(s) 69, 673, 757
Hpy8I GTNNAC 1 cut(s) 713
HpyAV CCTTC 2 cut(s) 105, 290
HpyCH4III ACNGT 2 cut(s) 349, 730
HpyCH4IV ACGT 1 cut(s) 599
HpyCH4V TGCA 2 cut(s) 538, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 350, 544, 581
HpyF3I CTNAG 2 cut(s) 665, 803
HpySE526I ACGT 1 cut(s) 599
Hsp92II CATG 6 cut(s) 439, 527, 644, 737, 760, 779
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 186, 654, 753
LmnI GCTCC 2 cut(s) 317, 581
Lsp1109I GCAGC 2 cut(s) 296, 766
LweI GCATC 2 cut(s) 362, 547
MaeI CTAG 1 cut(s) 410
MaeII ACGT 1 cut(s) 599
MaeIII GTNAC 5 cut(s) 10, 130, 422, 595, 646
MalI GATC 3 cut(s) 188, 656, 755
MboI GATC 3 cut(s) 186, 654, 753
MboII GAAGA 7 cut(s) 12, 233, 282, 316, 389, 559, 596
MhlI GDGCHC 2 cut(s) 523, 749
MluCI AATT 4 cut(s) 200, 335, 687, 719
MmeI TCCRAC 1 cut(s) 101
MseI TTAA 1 cut(s) 722
MspI CCGG 1 cut(s) 428
MspR9I CCNGG 5 cut(s) 316, 329, 402, 428, 429
Mva1269I GAATGC 1 cut(s) 265
MvaI CCWGG 3 cut(s) 316, 329, 402
MvnI CGCG 2 cut(s) 140, 233
MwoI GCNNNNNNNGC 4 cut(s) 309, 350, 544, 581
NciI CCSGG 2 cut(s) 428, 429
NdeII GATC 3 cut(s) 186, 654, 753
NlaIII CATG 6 cut(s) 439, 527, 644, 737, 760, 779
NlaIV GGNNCC 1 cut(s) 521
NmuCI GTSAC 1 cut(s) 422
PagI TCATGA 1 cut(s) 756
PcsI WCGNNNNNNNCGW 2 cut(s) 67, 369
PctI GAATGC 1 cut(s) 265
PfeI GAWTC 1 cut(s) 37
PfoI TCCNGGA 1 cut(s) 327
PkrI GCNGC 4 cut(s) 235, 311, 464, 781
Psp6I CCWGG 3 cut(s) 314, 327, 400
PspEI GGTNACC 1 cut(s) 422
PspGI CCWGG 3 cut(s) 314, 327, 400
PspN4I GGNNCC 1 cut(s) 521
PspOMI GGGCCC 1 cut(s) 519
PspPI GGNCC 2 cut(s) 519, 520
RsaI GTAC 1 cut(s) 414
RsaNI GTAC 1 cut(s) 413
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 4 cut(s) 234, 310, 463, 780
Sau3AI GATC 3 cut(s) 186, 654, 753
Sau96I GGNCC 2 cut(s) 519, 520
ScrFI CCNGG 5 cut(s) 316, 329, 402, 428, 429
SduI GDGCHC 2 cut(s) 523, 749
SetI ASST 8 cut(s) 30, 97, 381, 549, 602, 697, 718, 809
SfaNI GCATC 2 cut(s) 362, 547
SmaI CCCGGG 1 cut(s) 429
Sse9I AATT 4 cut(s) 200, 335, 687, 719
SsiI CCGC 3 cut(s) 138, 233, 462
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 5 cut(s) 314, 327, 400, 426, 427
StyI CCWWGG 1 cut(s) 784
TaaI ACNGT 2 cut(s) 349, 730
TaiI ACGT 1 cut(s) 602
TaqI TCGA 3 cut(s) 70, 88, 363
TasI AATT 4 cut(s) 200, 335, 687, 719
TatI WGTACW 1 cut(s) 412
TauI GCSGC 2 cut(s) 236, 465
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TscAI CASTG 1 cut(s) 754
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 2 cut(s) 309, 779
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 722
TspGWI ACGGA 1 cut(s) 168
TspMI CCCGGG 1 cut(s) 427
TspRI CASTG 1 cut(s) 754
XapI RAATTY 2 cut(s) 200, 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 782
XmaI CCCGGG 1 cut(s) 427
XspI CTAG 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.