Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009990

Physical Location & Seq

Genomic Coordinates Reverse (-)

17745683 .. 17750332

Gene Structure 4650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1111900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1935 bp

ATGTCAATCCTATCAGTTTCATCATCATCCTCATCATCCGCAGCCGTTGGATTATTTAATTCCCACATACCCGCCCTTCCCAATGCCTCATTTCTCACCCCACTCCCACTCCCACTCGTTGGGGCTCACCCAGCAAATGACCAGAAGAATAAGAAGAAGCAGAAGTGGAGCGCTGCCGCCGCCATTGGAGGAGGAGTCATGGCCACCAAGCTCACAGTCCTCAGGACCCCACACACTCTTTTCTGTGATGAATTGTTTAAACACAGTAGCTTCCAAGCAAAAACTAGAGATCCTCTGTGTTTCCGGGCACAATATATCTTATCCACTACATCACATTCAACATCAACAGCAACAACTACAGTGCTTGATATGGAGAGGCTCAGATTACCGTCTTTAGAAGTTAATTCTGATTCTCTGGCTTCAAGTAGACCGTGGACATACACAGGGGCAATCGGTCCTCCGACTGAGGCGAATTTTGGAGCAACTTTAGCTACAGAAACTCTTCTTATGAGTGAAGAGGCTGTAATAGAAGCTGCTGCCGCTGAAGCTGTCACTCTTGCAAAAGCAGCACTGAAGGTTGCAAAGGATGTGGCTATACTGGTTAGCAGTAGCACTAATCACTCTGCTAAACCAGAAAGTTCATCTCCAGTTTCTCCAGAGACTAAAGCTTTGCATTTCAATTGGGCTCAGCATATGGAAACCAAACAGGTTTCTAGAGTAGGAGCTTCCATGGGAGCTGAAACTGCATTTGTGGAAGATCATTTCATTCAATCTGCAATAAAAGAGTTGGAAGAATTGGAGCGAACAAATGAAGAATTTGAACTTCTGCAGGAGCAACTTTCCAAGGGTATAACTGCAAAATCGAGTCGTCAAACGGAAAGGAAGGCCAGAAGAGCAAAAGCAGCAGAAAAGGCTGCTGCAAGTGTTGTGTCCATGAAGTCTGGTTCAGCCACTAGAAAAAAGCGTTCTTCGGTACAAGAAATAGACCACTCTGATCCATTGCGTTACTTGAGATCAACTACCCACACTTCTCGCCTTCTGACTGCAAACGAAGAAATTGAGCTTTCTGAAGGAATCCAGGAACTACTGAAACTGGAAAAGCTGCATGAGGAGCTTGCACAAAGATGTGGTGGTCAGCCCACCATTGCACAATGGGCTGCAGCAGCAGGAGTTGATCAGAAGACTTTGAGGAAGCGTGTAAACTATGGTATTCTGTGCAAAGACAAAATGATCAAGAGCAACATAAGGCTTGTAATATCAATTGCAAAAAATTATCAGGGAGCTGGGATGAATCTTCAAGATCTTGTGCAGGAAGGATGTCGAGGCCTTGTAAGAGGTGCAGAGAAATTTGACGCTTCAAAGGGTTTTAAGTTCTCAACCTATGCACACTGGTGGATTAAACAGGCTGTTCGAAAATCTCTTTCTGACCAGTCCAGAACAATTCGCTTACCCTTTCACATGGTGGAGGCGACATATAGAGTTAGAGAGGCTAAAAAGCAACTGTACAGTTTAAATGGAAGACATCCTAACGATGAAGAAGTCGCAGAGGCAACAGGGCTGTCGATGAAAAGGCTTTCTGCTGTATTACTGACTCCAAAAGCCCCTAGATCTCTGGAGCAGAAAATTGGAATCAACCAGAATCTAAAACCTTCGGAAGTAATCTCAGATCCCGATGCAGAAACAGCAGAAGATCTGCTGATGAAAAAATTTATGAAGCGGGATTTGGAGAAGGTACTAGATAGTCTCAATCCTAGAGAGAAGCAGGTTGTCAGATGGAGGTTTGGATTGGAGGATGGAAGGATGAAGACACTGCAAGAGATAGGGGAGCTGATGGGAGTTAGTAGAGAGAGAATTAGACAAATAGAGTCATGTGCATTCCGAAAACTGAAGAACAAGAAAAGAACGAAACATTTACAGCAGTACGTGGTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006399 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009639 GO:0009657 GO:0009658 GO:0009889 GO:0009987 GO:0010114 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r1_2 Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

645

Amino Acids

71.29

Weight (kDa)

9.55

Isoelectric Point (pI)

45.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	410 - 480	3.1e-20	Sigma-70 region 2
Sigma70_r3	PF04539	489 - 564	1.6e-16	Sigma-70 region 3
Sigma70_r4	PF04545	578 - 630	1.3e-18	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009990)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08540
fragaria_vesca	FvH4_1g29160 FvH4_1g29160
malus_domestica	MD12G1111900.v1.1 MD14G1100900.v1.1
prunus_persica	Prupe.7G021900_v2.0.a1 Prupe.7G021900_v2.0.a1 Prupe.7G021900_v2.0.a1 Prupe.7G021900_v2.0.a1
pyrus_communis	pycom12g09170 pycom14g08810
rosa_chinensis	RchiOBHm_Chr3g0494351 RchiOBHm_Chr3g0494481
rosa_laevigata	RLG00000022672
rosa_multiflora	Rmu_sc0000152.1_g000008
rosa_roxburghii	Rroxscaffold_6G00391100
rosa_rugosa	Rorug03G0265600
rosa_samantha	Rh3BG350900 Rh3CG347600 Rh3DG350200
rosa_wichuraiana	Rw3G027560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1753
AccB7I	CCANNNNNTGG	1 cut(s)	119
AccI	GTMKAC	1 cut(s)	427
AciI	CCGC	6 cut(s)	39, 72, 177, 180, 540, 1717
AclWI	GGATC	3 cut(s)	284, 989, 1661
AcoI	YGGCCR	1 cut(s)	201
AcsI	RAATTY	4 cut(s)	472, 815, 1346, 1706
AcuI	CTGAAG	4 cut(s)	564, 593, 1089, 1907
AdeI	CACNNNGTG	1 cut(s)	1462
AfaI	GTAC	4 cut(s)	975, 1505, 1734, 1922
AfeI	AGCGCT	1 cut(s)	172
AfiI	CCNNNNNNNGG	1 cut(s)	119
AgsI	TTSAA	7 cut(s)	339, 423, 679, 770, 821, 1298, 1359
AjnI	CCWGG	1 cut(s)	1077
AjuI	GAANNNNNNNTTGG	2 cut(s)	1706, 1738
AleI	CACNNNNGTG	1 cut(s)	1390
Alw26I	GTCTC	2 cut(s)	653, 1748
AlwI	GGATC	3 cut(s)	284, 989, 1661
Aor51HI	AGCGCT	1 cut(s)	172
AoxI	GGCC	3 cut(s)	201, 885, 1324
ApoI	RAATTY	4 cut(s)	472, 815, 1346, 1706
ArsI	GACNNNNNNTTYG	2 cut(s)	652, 684
Asp700I	GAANNNNTTC	1 cut(s)	501
AspLEI	GCGC	1 cut(s)	173
AspS9I	GGNCC	2 cut(s)	225, 455
AsuC2I	CCSGG	1 cut(s)	305
AsuHPI	GGTGA	2 cut(s)	88, 119
AsuII	TTCGAA	1 cut(s)	1411
AvaII	GGWCC	2 cut(s)	225, 455
AxyI	CCTNAGG	1 cut(s)	221
BaeGI	GKGCMC	1 cut(s)	310
BalI	TGGCCA	1 cut(s)	203
BanII	GRGCYC	2 cut(s)	127, 688
BbsI	GAAGAC	3 cut(s)	1187, 1525, 1811
BccI	CCATC	3 cut(s)	1767, 1787, 1825
BceAI	ACGGC	1 cut(s)	29
BciT130I	CCWGG	1 cut(s)	1079
BclI	TGATCA	2 cut(s)	1174, 1230
BcnI	CCSGG	1 cut(s)	305
BcoDI	GTCTC	2 cut(s)	653, 1748
BfaI	CTAG	6 cut(s)	285, 714, 954, 1605, 1736, 1752
BfmI	CTRYAG	4 cut(s)	357, 492, 827, 1158
BfoI	RGCGCY	1 cut(s)	174
BfuAI	ACCTGC	1 cut(s)	1753
BglII	AGATCT	3 cut(s)	1300, 1607, 1690
BlpI	GCTNAGC	1 cut(s)	687
Bme1390I	CCNGG	2 cut(s)	305, 1079
Bme18I	GGWCC	2 cut(s)	225, 455
BmgT120I	GGNCC	2 cut(s)	225, 455
BmiI	GGNNCC	1 cut(s)	227
BmrFI	CCNGG	2 cut(s)	305, 1079
BmsI	GCATC	1 cut(s)	1663
BpiI	GAAGAC	3 cut(s)	1187, 1525, 1811
BpmI	CTGGAG	3 cut(s)	630, 639, 1634
Bpu1102I	GCTNAGC	1 cut(s)	687
Bpu14I	TTCGAA	1 cut(s)	1411
BpuEI	CTTGAG	1 cut(s)	1030
BpuMI	CCSGG	1 cut(s)	305
BsaAI	YACGTR	1 cut(s)	1924
BsaJI	CCNNGG	3 cut(s)	431, 729, 843
BsaXI	ACNNNNNCTCC	4 cut(s)	93, 123, 180, 210
Bsc4I	CCNNNNNNNGG	1 cut(s)	119
Bse1I	ACTGG	5 cut(s)	603, 647, 1098, 1394, 1429
Bse21I	CCTNAGG	1 cut(s)	221
Bse3DI	GCAATG	2 cut(s)	998, 1143
BseBI	CCWGG	1 cut(s)	1079
BseDI	CCNNGG	3 cut(s)	431, 729, 843
BseGI	GGATG	8 cut(s)	26, 35, 592, 1293, 1322, 1522, 1798, 1806
BseLI	CCNNNNNNNGG	1 cut(s)	119
BseMI	GCAATG	2 cut(s)	998, 1143
BseMII	CTCAG	5 cut(s)	235, 394, 456, 701, 1677
BseNI	ACTGG	5 cut(s)	603, 647, 1098, 1394, 1429
BseRI	GAGGAG	3 cut(s)	204, 207, 1124
BseSI	GKGCMC	1 cut(s)	310
BseYI	CCCAGC	2 cut(s)	130, 1283
BsgI	GTGCAG	2 cut(s)	1328, 1359
BshFI	GGCC	3 cut(s)	203, 887, 1326
BsiSI	CCGG	1 cut(s)	304
BslI	CCNNNNNNNGG	1 cut(s)	119
BsmAI	GTCTC	2 cut(s)	653, 1748
BsmI	GAATGC	1 cut(s)	1874
BsnI	GGCC	3 cut(s)	203, 887, 1326
Bsp119I	TTCGAA	1 cut(s)	1411
Bsp1286I	GDGCHC	3 cut(s)	127, 310, 688
Bsp1407I	TGTACA	1 cut(s)	1503
Bsp1720I	GCTNAGC	1 cut(s)	687
Bsp19I	CCATGG	1 cut(s)	729
BspACI	CCGC	6 cut(s)	39, 72, 177, 180, 540, 1717
BspANI	GGCC	3 cut(s)	203, 887, 1326
BspCNI	CTCAG	5 cut(s)	234, 393, 457, 700, 1676
BspLI	GGNNCC	1 cut(s)	227
BspMAI	CTGCAG	2 cut(s)	831, 1162
BspMI	ACCTGC	1 cut(s)	1753
BspPI	GGATC	3 cut(s)	284, 989, 1661
BspQI	GCTCTTC	1 cut(s)	886
BspT104I	TTCGAA	1 cut(s)	1411
BsrDI	GCAATG	2 cut(s)	998, 1143
BsrGI	TGTACA	1 cut(s)	1503
BsrI	ACTGG	5 cut(s)	603, 647, 1098, 1394, 1429
BssECI	CCNNGG	3 cut(s)	431, 729, 843
BssT1I	CCWWGG	2 cut(s)	729, 843
Bst2UI	CCWGG	1 cut(s)	1079
Bst4CI	ACNGT	7 cut(s)	217, 266, 361, 390, 432, 1503, 1508
Bst6I	CTCTTC	3 cut(s)	507, 510, 886
BstAUI	TGTACA	1 cut(s)	1503
BstBAI	YACGTR	1 cut(s)	1924
BstBI	TTCGAA	1 cut(s)	1411
BstC8I	GCNNGC	1 cut(s)	1116
BstDEI	CTNAG	5 cut(s)	221, 380, 465, 687, 1663
BstDSI	CCRYGG	2 cut(s)	431, 729
BstF5I	GGATG	8 cut(s)	26, 35, 592, 1293, 1322, 1522, 1798, 1806
BstH2I	RGCGCY	1 cut(s)	174
BstHHI	GCGC	1 cut(s)	173
BstMAI	GTCTC	2 cut(s)	653, 1748
BstNI	CCWGG	1 cut(s)	1079
BstSCI	CCNGG	2 cut(s)	303, 1077
BstSFI	CTRYAG	4 cut(s)	357, 492, 827, 1158
BstSLI	GKGCMC	1 cut(s)	310
BstV2I	GAAGAC	3 cut(s)	1187, 1525, 1811
BstX2I	RGATCY	5 cut(s)	289, 1300, 1607, 1666, 1690
BstYI	RGATCY	5 cut(s)	289, 1300, 1607, 1666, 1690
Bsu36I	CCTNAGG	1 cut(s)	221
BsuRI	GGCC	3 cut(s)	203, 887, 1326
BtgI	CCRYGG	2 cut(s)	431, 729
BtsCI	GGATG	8 cut(s)	26, 35, 592, 1293, 1322, 1522, 1798, 1806
BtsI	GCAGTG	1 cut(s)	1808
BtsIMutI	CAGTG	4 cut(s)	366, 569, 1387, 1808
BveI	ACCTGC	1 cut(s)	1753
Cac8I	GCNNGC	1 cut(s)	1116
CfoI	GCGC	1 cut(s)	173
Cfr13I	GGNCC	2 cut(s)	225, 455
CseI	GACGC	1 cut(s)	1361
Csp6I	GTAC	4 cut(s)	974, 1504, 1733, 1921
CspCI	CAANNNNNGTGG	2 cut(s)	570, 605
CviAII	CATG	6 cut(s)	199, 730, 934, 1106, 1459, 1869
CviQI	GTAC	4 cut(s)	974, 1504, 1733, 1921
DdeI	CTNAG	5 cut(s)	221, 380, 465, 687, 1663
DraI	TTTAAA	2 cut(s)	259, 1512
DraIII	CACNNNGTG	1 cut(s)	1462
EaeI	YGGCCR	1 cut(s)	201
Eam1104I	CTCTTC	3 cut(s)	507, 510, 886
EarI	CTCTTC	3 cut(s)	507, 510, 886
Eco130I	CCWWGG	2 cut(s)	729, 843
Eco147I	AGGCCT	1 cut(s)	1326
Eco24I	GRGCYC	2 cut(s)	127, 688
Eco47I	GGWCC	2 cut(s)	225, 455
Eco47III	AGCGCT	1 cut(s)	172
Eco57I	CTGAAG	4 cut(s)	564, 593, 1089, 1907
Eco81I	CCTNAGG	1 cut(s)	221
EcoO109I	RGGNCCY	1 cut(s)	225
EcoRII	CCWGG	1 cut(s)	1077
EcoT14I	CCWWGG	2 cut(s)	729, 843
EcoT38I	GRGCYC	2 cut(s)	127, 688
ErhI	CCWWGG	2 cut(s)	729, 843
FaeI	CATG	6 cut(s)	202, 733, 937, 1109, 1462, 1872
FatI	CATG	6 cut(s)	198, 729, 933, 1105, 1458, 1868
FauI	CCCGC	2 cut(s)	79, 1710
FauNDI	CATATG	1 cut(s)	693
FbaI	TGATCA	2 cut(s)	1174, 1230
FblI	GTMKAC	1 cut(s)	427
FokI	GGATG	8 cut(s)	13, 22, 599, 1300, 1329, 1509, 1805, 1813
FriOI	GRGCYC	2 cut(s)	127, 688
FspBI	CTAG	6 cut(s)	285, 714, 954, 1605, 1736, 1752
GlaI	GCGC	1 cut(s)	172
GsaI	CCCAGC	2 cut(s)	134, 1287
GsuI	CTGGAG	3 cut(s)	630, 639, 1634
HaeII	RGCGCY	1 cut(s)	174
HaeIII	GGCC	3 cut(s)	203, 887, 1326
HapII	CCGG	1 cut(s)	304
HgaI	GACGC	1 cut(s)	1361
HhaI	GCGC	1 cut(s)	173
Hin1II	CATG	6 cut(s)	202, 733, 937, 1109, 1462, 1872
Hin6I	GCGC	1 cut(s)	171
HinP1I	GCGC	1 cut(s)	171
HindIII	AAGCTT	1 cut(s)	666
HinfI	GANTC	9 cut(s)	195, 410, 865, 1074, 1291, 1591, 1629, 1639, 1865
HpaII	CCGG	1 cut(s)	304
HphI	GGTGA	2 cut(s)	88, 119
Hpy166II	GTNNAC	3 cut(s)	428, 435, 1201
Hpy188III	TCNNGA	8 cut(s)	223, 656, 714, 1234, 1298, 1434, 1613, 1670
Hpy8I	GTNNAC	3 cut(s)	428, 435, 1201
HpyAV	CCTTC	9 cut(s)	86, 568, 877, 1046, 1064, 1307, 1659, 1723, 1791
HpyCH4III	ACNGT	7 cut(s)	217, 266, 361, 390, 432, 1503, 1508
HpyCH4IV	ACGT	1 cut(s)	1923
HpyF3I	CTNAG	5 cut(s)	221, 380, 465, 687, 1663
HpySE526I	ACGT	1 cut(s)	1923
Hsp92II	CATG	6 cut(s)	202, 733, 937, 1109, 1462, 1872
HspAI	GCGC	1 cut(s)	171
Ksp22I	TGATCA	2 cut(s)	1174, 1230
LguI	GCTCTTC	1 cut(s)	886
LweI	GCATC	1 cut(s)	1663
MaeI	CTAG	6 cut(s)	285, 714, 954, 1605, 1736, 1752
MaeII	ACGT	1 cut(s)	1923
MaeIII	GTNAC	2 cut(s)	550, 1004
MfeI	CAATTG	2 cut(s)	679, 1260
MflI	RGATCY	5 cut(s)	289, 1300, 1607, 1666, 1690
MhlI	GDGCHC	3 cut(s)	127, 310, 688
MlsI	TGGCCA	1 cut(s)	203
MluNI	TGGCCA	1 cut(s)	203
MlyI	GAGTC	4 cut(s)	204, 874, 1585, 1874
MmeI	TCCRAC	3 cut(s)	28, 485, 768
Mox20I	TGGCCA	1 cut(s)	203
MroXI	GAANNNNTTC	1 cut(s)	501
MscI	TGGCCA	1 cut(s)	203
MseI	TTAA	6 cut(s)	57, 258, 402, 1368, 1398, 1511
MslI	CAYNNNNRTG	2 cut(s)	1123, 1390
Msp20I	TGGCCA	1 cut(s)	203
MspA1I	CMGCKG	1 cut(s)	542
MspI	CCGG	1 cut(s)	304
MspR9I	CCNGG	2 cut(s)	305, 1079
MssI	GTTTAAAC	1 cut(s)	259
MunI	CAATTG	2 cut(s)	679, 1260
Mva1269I	GAATGC	1 cut(s)	1874
MvaI	CCWGG	1 cut(s)	1079
NciI	CCSGG	1 cut(s)	305
NcoI	CCATGG	1 cut(s)	729
NdeI	CATATG	1 cut(s)	693
NlaIII	CATG	6 cut(s)	202, 733, 937, 1109, 1462, 1872
NlaIV	GGNNCC	1 cut(s)	227
NmuCI	GTSAC	1 cut(s)	550
NspV	TTCGAA	1 cut(s)	1411
OliI	CACNNNNGTG	1 cut(s)	1390
PceI	AGGCCT	1 cut(s)	1326
PciSI	GCTCTTC	1 cut(s)	886
PctI	GAATGC	1 cut(s)	1874
PdmI	GAANNNNTTC	1 cut(s)	501
PfeI	GAWTC	5 cut(s)	410, 1074, 1291, 1629, 1639
PflMI	CCANNNNNTGG	1 cut(s)	119
PfoI	TCCNGGA	1 cut(s)	1077
PleI	GAGTC	4 cut(s)	203, 873, 1585, 1873
PmeI	GTTTAAAC	1 cut(s)	259
PpsI	GAGTC	4 cut(s)	203, 873, 1585, 1873
Ppu21I	YACGTR	1 cut(s)	1924
PpuMI	RGGWCCY	1 cut(s)	225
Psp5II	RGGWCCY	1 cut(s)	225
Psp6I	CCWGG	1 cut(s)	1077
PspFI	CCCAGC	2 cut(s)	130, 1283
PspGI	CCWGG	1 cut(s)	1077
PspN4I	GGNNCC	1 cut(s)	227
PspPI	GGNCC	2 cut(s)	225, 455
PspPPI	RGGWCCY	1 cut(s)	225
PstI	CTGCAG	2 cut(s)	831, 1162
PsuI	RGATCY	5 cut(s)	289, 1300, 1607, 1666, 1690
RsaI	GTAC	4 cut(s)	975, 1505, 1734, 1922
RsaNI	GTAC	4 cut(s)	974, 1504, 1733, 1921
RseI	CAYNNNNRTG	2 cut(s)	1123, 1390
SapI	GCTCTTC	1 cut(s)	886
SaqAI	TTAA	6 cut(s)	57, 258, 402, 1368, 1398, 1511
Sau96I	GGNCC	2 cut(s)	225, 455
SchI	GAGTC	4 cut(s)	204, 874, 1585, 1874
ScrFI	CCNGG	2 cut(s)	305, 1079
SduI	GDGCHC	3 cut(s)	127, 310, 688
SfaNI	GCATC	1 cut(s)	1663
SfcI	CTRYAG	4 cut(s)	357, 492, 827, 1158
SfuI	TTCGAA	1 cut(s)	1411
SinI	GGWCC	2 cut(s)	225, 455
SmiMI	CAYNNNNRTG	2 cut(s)	1123, 1390
SmlI	CTYRAG	1 cut(s)	1009
SmoI	CTYRAG	1 cut(s)	1009
SseBI	AGGCCT	1 cut(s)	1326
SsiI	CCGC	6 cut(s)	39, 72, 177, 180, 540, 1717
SspMI	CTAG	6 cut(s)	285, 714, 954, 1605, 1736, 1752
StuI	AGGCCT	1 cut(s)	1326
StyD4I	CCNGG	2 cut(s)	303, 1077
StyI	CCWWGG	2 cut(s)	729, 843
TaaI	ACNGT	7 cut(s)	217, 266, 361, 390, 432, 1503, 1508
TaiI	ACGT	1 cut(s)	1926
TaqI	TCGA	4 cut(s)	863, 1321, 1411, 1562
TaqII	GACCGA	1 cut(s)	443
TatI	WGTACW	1 cut(s)	1503
TauI	GCSGC	3 cut(s)	179, 182, 542
TfiI	GAWTC	5 cut(s)	410, 1074, 1291, 1629, 1639
Tru1I	TTAA	6 cut(s)	57, 258, 402, 1368, 1398, 1511
Tru9I	TTAA	6 cut(s)	57, 258, 402, 1368, 1398, 1511
TscAI	CASTG	4 cut(s)	366, 576, 1394, 1815
TseFI	GTSAC	1 cut(s)	550
Tsp45I	GTSAC	1 cut(s)	550
TspGWI	ACGGA	1 cut(s)	890
TspRI	CASTG	4 cut(s)	366, 576, 1394, 1815
Van91I	CCANNNNNTGG	1 cut(s)	119
VpaK11BI	GGWCC	2 cut(s)	225, 455
XapI	RAATTY	4 cut(s)	472, 815, 1346, 1706
XbaI	TCTAGA	1 cut(s)	713
XmiI	GTMKAC	1 cut(s)	427
XmnI	GAANNNNTTC	1 cut(s)	501
XspI	CTAG	6 cut(s)	285, 714, 954, 1605, 1736, 1752

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.